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Biogenex biotinylated secondary antibody
Biotinylated Secondary Antibody, supplied by Biogenex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotinylated secondary antibody/product/Biogenex
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
biotinylated secondary antibody - by Bioz Stars, 2021-03
86/100 stars

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Incubation:

Article Title: Pten Deletion in Adult Neural Stem/Progenitor Cells Enhances Constitutive Neurogenesis
Article Snippet: After washing in deionized water, the slides were transferred to either PBS (pH 7.4) or TBST for 5 min. For DAB staining, slides were first blocked with 5% normal goat serum then incubated with primary antibody overnight at 4°C. .. Following three 5 min washes in either PBS or TBST, slides were incubated with biotinylated secondary antibody (1:200, Biogenex) for 30 min at room temperature. .. Amplification was performed with a horseradish peroxidase system (Vectastain ABC kit, Vector, PK-6100) using a liquid DAB peroxidase substrate (Biogenex, HK130-5K).

Article Title: Analysis of the immunoexpression of Ki-67 and Bcl-2 in the pericoronal tissues of impacted teeth, dentigerous cysts and gingiva using software image analysis
Article Snippet: The slides were incubated with the Super Enhancer in a moist chamber for 25 minutes. .. Following a PBS wash for three minutes, thrice, the sections were incubated with biotinylated secondary antibody at 1:200 dilutions at room temperature, for 30 minutes (Biogenex; USA). .. Following a PBS wash for five minutes, thrice, the sections were incubated with streptavidin-peroxidase conjugate (Biogenex; USA); at a concentration of 1:200 for 30 minutes, and detected using 0.05% 3;3-diaminobenzidine tetra-hydrochloride (DAB) (Dakocytomation; Glostrup; Denmark).

Article Title: Immunohistochemical profiling of Ameloblastomas using cytokeratin, vimentin, smooth muscle actin, CD34 and S100
Article Snippet: Further incubations with pre-diluted ready to use primary mouse monoclonal anti-cytokeratin antibody (AE1/AE3) (Biogenex, San Ramon), monoclonal mouse anti-vimentin antibody (V9) (Biogenex, San Ramon), monoclonal mouse anti-smooth muscle actin antibody (IA4) (Biogenex, San Ramon), monoclonal mouse anti CD34 antibody (Q Bend/10) (Biogenex, San Ramon), monoclonal mouse anti S100 antibody (N 1573) (DAKO Carpenteria, CA, USA) was performed at 37° C for 30 min. .. Sections were washed again and incubated with biotinylated secondary antibody (Biogenex, San Ramon) for 30 min followed by the streptavidin-biotin-peroxidase (Biogenex, San Ramon) for 30 min at room temperature. ..

Article Title: Newer Insights Into Premeiotic Development of Germ Cells in Adult Human Testis Using Oct-4 as a Stem Cell Marker
Article Snippet: .. After washing with PBS, the cells were incubated with the biotinylated secondary antibody (anti-rabbit IgG for polyclonal antibody and anti-mouse IgG for MAb) for 30 min followed by avidin–biotin complex formation step for 30 min; the cells were then extensively washed with PBS and then detected using DAB (BioGenex; San Ramon, CA) and counterstained with hematoxylin. .. For immunohistochemical localization, the paraffin sections were deparaffinized and rehydrated through a graded ethanol series.

Article Title: Molecular dissection of ALS-associated toxicity of SOD1 in transgenic mice using an exon-fusion approach
Article Snippet: .. The specific primary antibody of interest (such as f-SOD1) was applied to the slides and incubated at room temperature for 1 h. After rinsing the slides, a biotinylated secondary antibody in PBS was applied, and the slides were incubated at room temperature for 30 min. After removing the excess secondary antibody, a peroxidase-conjugated streptavidin (BioGenex, San Ramon, CA, USA) was applied, and the slides were incubated for 30 min at room temperature. ..

Article Title: PTEN dosage is essential for neurofibroma development and malignant transformation
Article Snippet: After washing in deionized water, the slides were then transferred to either PBS (pH 7.4) or TBST for 5 min. For DAB staining, slides were first blocked with 5% normal goat serum, then incubated with primary antibody overnight at 4 °C. .. Following three 5-min washes in either PBS or TBST, slides were incubated with biotinylated secondary antibody (1:200; Biogenex) for 30 min at room temperature. .. Amplification was performed with a horseradish peroxidase system (Vectastain ABC kit, PK-6100; Vector Laboratories) using a liquid DAB peroxidase substrate (HK130–5K; Biogenex).

Avidin-Biotin Assay:

Article Title: Newer Insights Into Premeiotic Development of Germ Cells in Adult Human Testis Using Oct-4 as a Stem Cell Marker
Article Snippet: .. After washing with PBS, the cells were incubated with the biotinylated secondary antibody (anti-rabbit IgG for polyclonal antibody and anti-mouse IgG for MAb) for 30 min followed by avidin–biotin complex formation step for 30 min; the cells were then extensively washed with PBS and then detected using DAB (BioGenex; San Ramon, CA) and counterstained with hematoxylin. .. For immunohistochemical localization, the paraffin sections were deparaffinized and rehydrated through a graded ethanol series.

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  • 86
    Biogenex biotinylated goat anti rabbit secondary antibodies
    Intratibial tumors of MDA-MB-435-LUC cells in nude mice were treated with Ad5-Δ24-sOPG-Fc-RGD, Ad5-Δ24RGD or PBS. A , bioluminescent imaging on days 4 and 9. B , histology of consecutive sections, shown at 12.5X and 200X. H E: hematoxylin and eosin; TRAP: detection of TRAP osteoclast marker protein with 1.1 mg/ml fast red TR salt in 0.2 M acetate buffer; IHC; immunohistochemistry for Ad5 capsid proteins using rabbit Ad5 antiserum, <t>biotinylated</t> goat anti-rabbit secondary antibodies, and streptavidin peroxidase. Tissue samples were harvested 10 days after treatment.
    Biotinylated Goat Anti Rabbit Secondary Antibodies, supplied by Biogenex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated goat anti rabbit secondary antibodies/product/Biogenex
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated goat anti rabbit secondary antibodies - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Biogenex biotinylated anti mouse secondary antibody
    Intratibial tumors of MDA-MB-435-LUC cells in nude mice were treated with Ad5-Δ24-sOPG-Fc-RGD, Ad5-Δ24RGD or PBS. A , bioluminescent imaging on days 4 and 9. B , histology of consecutive sections, shown at 12.5X and 200X. H E: hematoxylin and eosin; TRAP: detection of TRAP osteoclast marker protein with 1.1 mg/ml fast red TR salt in 0.2 M acetate buffer; IHC; immunohistochemistry for Ad5 capsid proteins using rabbit Ad5 antiserum, <t>biotinylated</t> goat anti-rabbit secondary antibodies, and streptavidin peroxidase. Tissue samples were harvested 10 days after treatment.
    Biotinylated Anti Mouse Secondary Antibody, supplied by Biogenex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated anti mouse secondary antibody/product/Biogenex
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated anti mouse secondary antibody - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Biogenex biotinylated secondary antibody
    Intratibial tumors of MDA-MB-435-LUC cells in nude mice were treated with Ad5-Δ24-sOPG-Fc-RGD, Ad5-Δ24RGD or PBS. A , bioluminescent imaging on days 4 and 9. B , histology of consecutive sections, shown at 12.5X and 200X. H E: hematoxylin and eosin; TRAP: detection of TRAP osteoclast marker protein with 1.1 mg/ml fast red TR salt in 0.2 M acetate buffer; IHC; immunohistochemistry for Ad5 capsid proteins using rabbit Ad5 antiserum, <t>biotinylated</t> goat anti-rabbit secondary antibodies, and streptavidin peroxidase. Tissue samples were harvested 10 days after treatment.
    Biotinylated Secondary Antibody, supplied by Biogenex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated secondary antibody/product/Biogenex
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary antibody - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

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    Intratibial tumors of MDA-MB-435-LUC cells in nude mice were treated with Ad5-Δ24-sOPG-Fc-RGD, Ad5-Δ24RGD or PBS. A , bioluminescent imaging on days 4 and 9. B , histology of consecutive sections, shown at 12.5X and 200X. H E: hematoxylin and eosin; TRAP: detection of TRAP osteoclast marker protein with 1.1 mg/ml fast red TR salt in 0.2 M acetate buffer; IHC; immunohistochemistry for Ad5 capsid proteins using rabbit Ad5 antiserum, biotinylated goat anti-rabbit secondary antibodies, and streptavidin peroxidase. Tissue samples were harvested 10 days after treatment.

    Journal: Cancer gene therapy

    Article Title: Arming a Replicating Adenovirus with Osteoprotegerin Reduces the Tumor Burden in a Murine Model of Osteolytic Bone Metastases of Breast Cancer

    doi: 10.1038/cgt.2010.47

    Figure Lengend Snippet: Intratibial tumors of MDA-MB-435-LUC cells in nude mice were treated with Ad5-Δ24-sOPG-Fc-RGD, Ad5-Δ24RGD or PBS. A , bioluminescent imaging on days 4 and 9. B , histology of consecutive sections, shown at 12.5X and 200X. H E: hematoxylin and eosin; TRAP: detection of TRAP osteoclast marker protein with 1.1 mg/ml fast red TR salt in 0.2 M acetate buffer; IHC; immunohistochemistry for Ad5 capsid proteins using rabbit Ad5 antiserum, biotinylated goat anti-rabbit secondary antibodies, and streptavidin peroxidase. Tissue samples were harvested 10 days after treatment.

    Article Snippet: Another consecutive section was immunostained for Ad5 capsid proteins using rabbit Ad5 antiserum (Cocalico Biologicals, Inc., Reamstown, PA), biotinylated goat anti-rabbit secondary antibodies, and streptavidin peroxidase (Biogenex Laboratories, Inc., San Ramon, CA) with diaminobenzidine (Lab Vision Corporation, Fremont, CA) as substrate.

    Techniques: Multiple Displacement Amplification, Mouse Assay, Imaging, Marker, Immunohistochemistry