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Agilent technologies biotinylated secondary antibody
Biotinylated Secondary Antibody, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotinylated secondary antibody/product/Agilent technologies
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
biotinylated secondary antibody - by Bioz Stars, 2021-03
86/100 stars

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Binding Assay:

Article Title: Preliminary Assessment of the Mucosal Toxicity of Tea Tree (Melaleuca alternifolia) and Rosemary (Rosmarinus officinalis) Essential Oils on Novel Porcine Uterus Models
Article Snippet: For antigen retrieval, slides were incubated for 20 min at 37 °C with protease XIV enzyme (protease from Streptomyces griseus, P5147, Sigma-Aldrich, St Louis, Missouri, USA). .. Sites of primary antibody binding were identified by incubation with appropriate biotinylated secondary antibody (Code No. E 0432, Dako—Glostrup, Danimarca) at a dilution of 1:200 in blocking solution. .. Sections were incubated with a commercial streptavidin-biotin-peroxidase kit (Vectastain Elite ABC Kit, Vector Laboratories, Burlingame, CA, USA) for 30 min; 3,3’-diaminobenzidine (DAB, Diagnostic BioSystems, Pleasanton, CA, USA) was used as chromogen.

Incubation:

Article Title: Preliminary Assessment of the Mucosal Toxicity of Tea Tree (Melaleuca alternifolia) and Rosemary (Rosmarinus officinalis) Essential Oils on Novel Porcine Uterus Models
Article Snippet: For antigen retrieval, slides were incubated for 20 min at 37 °C with protease XIV enzyme (protease from Streptomyces griseus, P5147, Sigma-Aldrich, St Louis, Missouri, USA). .. Sites of primary antibody binding were identified by incubation with appropriate biotinylated secondary antibody (Code No. E 0432, Dako—Glostrup, Danimarca) at a dilution of 1:200 in blocking solution. .. Sections were incubated with a commercial streptavidin-biotin-peroxidase kit (Vectastain Elite ABC Kit, Vector Laboratories, Burlingame, CA, USA) for 30 min; 3,3’-diaminobenzidine (DAB, Diagnostic BioSystems, Pleasanton, CA, USA) was used as chromogen.

Article Title: Substance P modulates bone remodeling properties of murine osteoblasts and osteoclasts
Article Snippet: Sections were incubated with 1% BSA and 5% swine serum for 1 h at RT to block unspecific binding sites and subsequently incubated with primary antibodies against Runx2 (1:1000, ab192256, Abcam) over night at 4 °C in blocking solution and afterwards washed with PBS. .. Secondary biotinylated antibody (#E0431, DAKO, 1:500, 37 °C, 1 hour) was used for detection followed by incubation with Strepavidin-HRP (#P0397, DAKO, 1:300, 37 °C, 1 h). .. Afterwards sections were washed twice with PBS and incubated with DAB Enhanced Liquid Substrate System (# D3939, Sigma-Aldrich, Steinheim, Germany) at RT for 10 min.

Article Title: The Expression of the SLIT–ROBO Family in Adult Patients with Acute Myeloid Leukemia
Article Snippet: Antibody diluent (S080983-2, DAKO, Denmark) was used for all solutions. .. After washing in Tris-buffer, the sections were incubated for 30 min with a biotinylated secondary antibody (K069011-2, DAKO, Denmark) and rinsed in Tris-buffer again. .. The slides were subsequently incubated with horseradish-conjugated streptavidin for 30 min (K069011-2, DAKO, Denmark) and washed in Tris-buffer for 5 min.

Blocking Assay:

Article Title: Preliminary Assessment of the Mucosal Toxicity of Tea Tree (Melaleuca alternifolia) and Rosemary (Rosmarinus officinalis) Essential Oils on Novel Porcine Uterus Models
Article Snippet: For antigen retrieval, slides were incubated for 20 min at 37 °C with protease XIV enzyme (protease from Streptomyces griseus, P5147, Sigma-Aldrich, St Louis, Missouri, USA). .. Sites of primary antibody binding were identified by incubation with appropriate biotinylated secondary antibody (Code No. E 0432, Dako—Glostrup, Danimarca) at a dilution of 1:200 in blocking solution. .. Sections were incubated with a commercial streptavidin-biotin-peroxidase kit (Vectastain Elite ABC Kit, Vector Laboratories, Burlingame, CA, USA) for 30 min; 3,3’-diaminobenzidine (DAB, Diagnostic BioSystems, Pleasanton, CA, USA) was used as chromogen.

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  • 86
    Agilent technologies biotinylated goat anti mouse secondary antibody
    Scheme of enzyme-enhanced LFIC for VCP detection <t>Biotinylated</t> gold nanoparticles tethered with streptavidin-bearing HRP at the capture site in the lateral flow strip to generate a signal upon interaction with TMB.
    Biotinylated Goat Anti Mouse Secondary Antibody, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated goat anti mouse secondary antibody/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated goat anti mouse secondary antibody - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Agilent technologies biotinylated anti mouse
    The vaccine-induced autologous neutralizing mAbs target a V2 epitope (A) Neutralization potency (IC 50 , μg/ml) of the NHP mAbs with selected N-glycan modifications of 16055 Env compared to wt. (B) Plasma neutralization titers (ID 50 ) from each animal against wt 16055 compared to N301 N-glycan deleted virus. (C) Binding of <t>biotinylated</t> NHP mAbs to 16055 NFL TD CC trimers (His-captured) in the presence of non-biotinylated mAb competitors (left column) as assessed by ELISA, where 0% competition was the absorbance measured with no competitor present. ( D ) Structure of the V1 (cyan) V2 (green) V3 (orange) region from the clade G X1193.c1 trimer crystal structure (PDB 5FYJ, top left) with strands A, B, C/C′, D and loops V2a and V2b indicated; orientation within one protomer of the trimer shown below. Bar diagram schematic indicating locations of the variable region deletions in 16055 gp120 to map binding (top right) with relative binding to each deletion mutant compared to wt gp120 shown below; +, binding; –, no binding. ( E .
    Biotinylated Anti Mouse, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated anti mouse/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated anti mouse - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Agilent technologies biotinylated secondary antibodies
    Labeling for apoptotic nuclei. End labeling with <t>biotinylated</t> nucleotides was visualized with a diaminobenzidine chromagen with a methylene green counterstain. End labeling occurred after ( a ) TDP-43 injections, but not after ( b ) control injections ( c ) higher magnification of end labeling as in a . Interval of 2 weeks and equal vector doses of 3 × 10 10 vector genomes. a,b , bar = 34 µm; c , bar = 21 µm.
    Biotinylated Secondary Antibodies, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated secondary antibodies/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary antibodies - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Agilent technologies biotinylated secondary antibody
    Mac-1 and CD147 interaction. Mac-1/CD147 interaction was assessed using an ELISA with recombinant Mac-1 or BSA (c ontrol) coating. Recombinant CD147 was added in ascending concentrations for 1 h. Evaluation was facilitated using a <t>biotinylated</t> secondary anti-CD147 antibody with a streptavidin/HRP complex. Changes in absorption of 3,3′,5,5′-tetramethylbenzidine were measured at 450 nm. Measurements from 6 independent experiments were analyzed, * indicates p
    Biotinylated Secondary Antibody, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated secondary antibody/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary antibody - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    Scheme of enzyme-enhanced LFIC for VCP detection Biotinylated gold nanoparticles tethered with streptavidin-bearing HRP at the capture site in the lateral flow strip to generate a signal upon interaction with TMB.

    Journal: Oncotarget

    Article Title: Point-of-care test for cervical cancer in LMICs

    doi: 10.18632/oncotarget.7709

    Figure Lengend Snippet: Scheme of enzyme-enhanced LFIC for VCP detection Biotinylated gold nanoparticles tethered with streptavidin-bearing HRP at the capture site in the lateral flow strip to generate a signal upon interaction with TMB.

    Article Snippet: Biotinylated goat anti-mouse secondary antibody (GAM IgG) conjugated to HRP was purchased from Dakocytomation (Carpinteria, CA).

    Techniques: Flow Cytometry, Stripping Membranes

    The vaccine-induced autologous neutralizing mAbs target a V2 epitope (A) Neutralization potency (IC 50 , μg/ml) of the NHP mAbs with selected N-glycan modifications of 16055 Env compared to wt. (B) Plasma neutralization titers (ID 50 ) from each animal against wt 16055 compared to N301 N-glycan deleted virus. (C) Binding of biotinylated NHP mAbs to 16055 NFL TD CC trimers (His-captured) in the presence of non-biotinylated mAb competitors (left column) as assessed by ELISA, where 0% competition was the absorbance measured with no competitor present. ( D ) Structure of the V1 (cyan) V2 (green) V3 (orange) region from the clade G X1193.c1 trimer crystal structure (PDB 5FYJ, top left) with strands A, B, C/C′, D and loops V2a and V2b indicated; orientation within one protomer of the trimer shown below. Bar diagram schematic indicating locations of the variable region deletions in 16055 gp120 to map binding (top right) with relative binding to each deletion mutant compared to wt gp120 shown below; +, binding; –, no binding. ( E .

    Journal: Immunity

    Article Title: Particulate array of well-ordered HIV clade C Env trimers elicits neutralizing antibodies that display a unique V2 cap approach

    doi: 10.1016/j.immuni.2017.04.021

    Figure Lengend Snippet: The vaccine-induced autologous neutralizing mAbs target a V2 epitope (A) Neutralization potency (IC 50 , μg/ml) of the NHP mAbs with selected N-glycan modifications of 16055 Env compared to wt. (B) Plasma neutralization titers (ID 50 ) from each animal against wt 16055 compared to N301 N-glycan deleted virus. (C) Binding of biotinylated NHP mAbs to 16055 NFL TD CC trimers (His-captured) in the presence of non-biotinylated mAb competitors (left column) as assessed by ELISA, where 0% competition was the absorbance measured with no competitor present. ( D ) Structure of the V1 (cyan) V2 (green) V3 (orange) region from the clade G X1193.c1 trimer crystal structure (PDB 5FYJ, top left) with strands A, B, C/C′, D and loops V2a and V2b indicated; orientation within one protomer of the trimer shown below. Bar diagram schematic indicating locations of the variable region deletions in 16055 gp120 to map binding (top right) with relative binding to each deletion mutant compared to wt gp120 shown below; +, binding; –, no binding. ( E .

    Article Snippet: Secondary antibodies were: biotinylated anti-mouse (Dako), biotinylated anti-goat or biotinylated anti-rabbit (Jackson ImmunoResearch).

    Techniques: Neutralization, Binding Assay, Enzyme-linked Immunosorbent Assay, Mutagenesis

    Labeling for apoptotic nuclei. End labeling with biotinylated nucleotides was visualized with a diaminobenzidine chromagen with a methylene green counterstain. End labeling occurred after ( a ) TDP-43 injections, but not after ( b ) control injections ( c ) higher magnification of end labeling as in a . Interval of 2 weeks and equal vector doses of 3 × 10 10 vector genomes. a,b , bar = 34 µm; c , bar = 21 µm.

    Journal: Molecular Therapy: the Journal of the American Society of Gene Therapy

    Article Title: Mimicking Aspects of Frontotemporal Lobar Degeneration and Lou Gehrig's Disease in Rats via TDP-43 Overexpression

    doi: 10.1038/mt.2009.3

    Figure Lengend Snippet: Labeling for apoptotic nuclei. End labeling with biotinylated nucleotides was visualized with a diaminobenzidine chromagen with a methylene green counterstain. End labeling occurred after ( a ) TDP-43 injections, but not after ( b ) control injections ( c ) higher magnification of end labeling as in a . Interval of 2 weeks and equal vector doses of 3 × 10 10 vector genomes. a,b , bar = 34 µm; c , bar = 21 µm.

    Article Snippet: Biotinylated secondary antibodies for peroxidase staining were from DAKO Cytomation (1:2,000; Carpinteria, CA), incubated for 1 hour at room temperature.

    Techniques: Labeling, End Labeling, Plasmid Preparation

    Mac-1 and CD147 interaction. Mac-1/CD147 interaction was assessed using an ELISA with recombinant Mac-1 or BSA (c ontrol) coating. Recombinant CD147 was added in ascending concentrations for 1 h. Evaluation was facilitated using a biotinylated secondary anti-CD147 antibody with a streptavidin/HRP complex. Changes in absorption of 3,3′,5,5′-tetramethylbenzidine were measured at 450 nm. Measurements from 6 independent experiments were analyzed, * indicates p

    Journal: Biomolecules

    Article Title: CD147 is a Novel Interaction Partner of Integrin αMβ2 Mediating Leukocyte and Platelet Adhesion

    doi: 10.3390/biom10040541

    Figure Lengend Snippet: Mac-1 and CD147 interaction. Mac-1/CD147 interaction was assessed using an ELISA with recombinant Mac-1 or BSA (c ontrol) coating. Recombinant CD147 was added in ascending concentrations for 1 h. Evaluation was facilitated using a biotinylated secondary anti-CD147 antibody with a streptavidin/HRP complex. Changes in absorption of 3,3′,5,5′-tetramethylbenzidine were measured at 450 nm. Measurements from 6 independent experiments were analyzed, * indicates p

    Article Snippet: Recombinant CD147 was added in increasing concentrations (0–20 µg/mL) for 1 h. After removing CD147 and gentle washing, the wells were incubated with an anti-CD147 antibody (mouse-anti CD147, Abcam, Cambridge, UK) followed by a biotinylated secondary antibody (polyclonal rabbit anti mouse, Dako, Glostrup, Denmark) and a streptavidin/HRP complex (Life technologies, Carlsbad, CA, USA).

    Techniques: Enzyme-linked Immunosorbent Assay, Recombinant