biotinylated secondary antibody  (Agilent technologies)

Journal: Oncotarget

Article Title: Point-of-care test for cervical cancer in LMICs

doi: 10.18632/oncotarget.7709

Figure Lengend Snippet: Scheme of enzyme-enhanced LFIC for VCP detection Biotinylated gold nanoparticles tethered with streptavidin-bearing HRP at the capture site in the lateral flow strip to generate a signal upon interaction with TMB.

Article Snippet: Biotinylated goat anti-mouse secondary antibody (GAM IgG) conjugated to HRP was purchased from Dakocytomation (Carpinteria, CA).

Techniques: Flow Cytometry, Stripping Membranes

Journal: Immunity

Article Title: Particulate array of well-ordered HIV clade C Env trimers elicits neutralizing antibodies that display a unique V2 cap approach

doi: 10.1016/j.immuni.2017.04.021

Figure Lengend Snippet: The vaccine-induced autologous neutralizing mAbs target a V2 epitope (A) Neutralization potency (IC 50 , μg/ml) of the NHP mAbs with selected N-glycan modifications of 16055 Env compared to wt. (B) Plasma neutralization titers (ID 50 ) from each animal against wt 16055 compared to N301 N-glycan deleted virus. (C) Binding of biotinylated NHP mAbs to 16055 NFL TD CC trimers (His-captured) in the presence of non-biotinylated mAb competitors (left column) as assessed by ELISA, where 0% competition was the absorbance measured with no competitor present. ( D ) Structure of the V1 (cyan) V2 (green) V3 (orange) region from the clade G X1193.c1 trimer crystal structure (PDB 5FYJ, top left) with strands A, B, C/C′, D and loops V2a and V2b indicated; orientation within one protomer of the trimer shown below. Bar diagram schematic indicating locations of the variable region deletions in 16055 gp120 to map binding (top right) with relative binding to each deletion mutant compared to wt gp120 shown below; +, binding; –, no binding. ( E .

Article Snippet: Secondary antibodies were: biotinylated anti-mouse (Dako), biotinylated anti-goat or biotinylated anti-rabbit (Jackson ImmunoResearch).

Techniques: Neutralization, Binding Assay, Enzyme-linked Immunosorbent Assay, Mutagenesis

Journal: Molecular Therapy: the Journal of the American Society of Gene Therapy

Article Title: Mimicking Aspects of Frontotemporal Lobar Degeneration and Lou Gehrig's Disease in Rats via TDP-43 Overexpression

doi: 10.1038/mt.2009.3

Figure Lengend Snippet: Labeling for apoptotic nuclei. End labeling with biotinylated nucleotides was visualized with a diaminobenzidine chromagen with a methylene green counterstain. End labeling occurred after ( a ) TDP-43 injections, but not after ( b ) control injections ( c ) higher magnification of end labeling as in a . Interval of 2 weeks and equal vector doses of 3 × 10 10 vector genomes. a,b , bar = 34 µm; c , bar = 21 µm.

Article Snippet: Biotinylated secondary antibodies for peroxidase staining were from DAKO Cytomation (1:2,000; Carpinteria, CA), incubated for 1 hour at room temperature.

Techniques: Labeling, End Labeling, Plasmid Preparation

Journal: Biomolecules

Article Title: CD147 is a Novel Interaction Partner of Integrin αMβ2 Mediating Leukocyte and Platelet Adhesion

doi: 10.3390/biom10040541

Figure Lengend Snippet: Mac-1 and CD147 interaction. Mac-1/CD147 interaction was assessed using an ELISA with recombinant Mac-1 or BSA (c ontrol) coating. Recombinant CD147 was added in ascending concentrations for 1 h. Evaluation was facilitated using a biotinylated secondary anti-CD147 antibody with a streptavidin/HRP complex. Changes in absorption of 3,3′,5,5′-tetramethylbenzidine were measured at 450 nm. Measurements from 6 independent experiments were analyzed, * indicates p

Article Snippet: Recombinant CD147 was added in increasing concentrations (0–20 µg/mL) for 1 h. After removing CD147 and gentle washing, the wells were incubated with an anti-CD147 antibody (mouse-anti CD147, Abcam, Cambridge, UK) followed by a biotinylated secondary antibody (polyclonal rabbit anti mouse, Dako, Glostrup, Denmark) and a streptavidin/HRP complex (Life technologies, Carlsbad, CA, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Recombinant