biotinylated secondary antibody  (Abcam)

 
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    Name:
    Rabbit Anti Goat IgG H L HRP
    Description:

    Catalog Number:
    ab6741
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    None
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    Structured Review

    Abcam biotinylated secondary antibody

    https://www.bioz.com/result/biotinylated secondary antibody/product/Abcam
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary antibody - by Bioz Stars, 2021-03
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: TFF3 and TFF1 expression levels are elevated in colorectal cancer and promote the malignant behavior of colon cancer by activating the EMT process
    Article Snippet: Subsequently, the membranes were probed with primary antibodies against TFF1 (1:500, ab92377) TFF3 (1:300, ab108599) and Twist1 (1:500, ab50581), E cadherin (1:500, ab40772), Vimentin (1:500, ab92547), Snail (1:500, ab53519), β-actin (1:2,500, ab8226) (all from Abcam) in blocking solution overnight at 4°C. .. Following a TBS-Tween wash, the membranes were incubated with goat anti-rabbit secondary antibody (HRP; 1:10,000, ab6721) or rabbit anti-goat secondary antibody (HRP; 1:5,000, ab6741) (both from Abcam) in blocking solution for 1 h at room temperature. .. Immunoreactive proteins were detected using enhanced chemiluminescence kit (Thermo Scientific Pierce ECL Substrate).

    Article Title: Optimization of Formulations Consisting of Layered Double Hydroxide Nanoparticles and Small Interfering RNA for Efficient Knockdown of the Target Gene
    Article Snippet: PD-L1 RabMab antibodies (ab205921) (Abcam, USA; at dilution of 1:800–1000) were applied overnight at 4 °C. .. The membrane was washed with TBST and then incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (goat antirabbit IgG H & L (HRP), ab97051) (Abcam, USA; at dilution 1:5000) for 2 h. After washing, protein bands were visualized using Clarity western ECL substrate (Bio-Rad) and analyzed using ImageJ v1.40 software. .. Data were analyzed by one-way analysis of variance, followed by multiple comparisons using Tukey’s test within GraphPad Prism 6 software.

    Article Title: Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF-α
    Article Snippet: .. Following washing, membranes were incubated with the following corresponding secondary antibodies at 37°C for 60 min: Horseradish peroxidase (HRP)-tagged goat anti-mouse IgG H & L (cat. no. ab6789; 1:7,000; Abcam), HRP-tagged rabbit anti-mouse IgG H & L (cat. no. ab6728; 1:8,000; Abcam) and HRP-tagged rabbit anti-goat IgG H & L (cat. no. ab97100; 1:8,000; Abcam). .. Finally, the blots were visualized by enhanced chemiluminescent reagent (EMD Millipore, Billerica, MA, USA).

    Blocking Assay:

    Article Title: TFF3 and TFF1 expression levels are elevated in colorectal cancer and promote the malignant behavior of colon cancer by activating the EMT process
    Article Snippet: Subsequently, the membranes were probed with primary antibodies against TFF1 (1:500, ab92377) TFF3 (1:300, ab108599) and Twist1 (1:500, ab50581), E cadherin (1:500, ab40772), Vimentin (1:500, ab92547), Snail (1:500, ab53519), β-actin (1:2,500, ab8226) (all from Abcam) in blocking solution overnight at 4°C. .. Following a TBS-Tween wash, the membranes were incubated with goat anti-rabbit secondary antibody (HRP; 1:10,000, ab6721) or rabbit anti-goat secondary antibody (HRP; 1:5,000, ab6741) (both from Abcam) in blocking solution for 1 h at room temperature. .. Immunoreactive proteins were detected using enhanced chemiluminescence kit (Thermo Scientific Pierce ECL Substrate).

    other:

    Article Title: An Antibody-Immobilized Silica Inverse Opal Nanostructure for Label-Free Optical Biosensors
    Article Snippet: Horseradish Peroxidase (HRP) conjugated anti-rabbit secondary antibody (Goat Anti-Rabbit IgG H & L (HRP)) was purchased from Abcam Co. (Cambridge, UK).

    Western Blot:

    Article Title: Gtr/Ego-independent TORC1 activation is achieved through a glutamine-sensitive interaction with Pib2 on the vacuolar membrane
    Article Snippet: Each sample was mixed with 50 μl of 4×loading buffer (800 mM Tris-Cl (pH 6.8) (Sigma), 6% SDS (nacalai tesque), 400 mM dithiothreitol (Wako), 8 M urea (Wako), 0.04% bromophenol blue(Sigma)) and subjected to SDS-PAGE and western blot analysis. .. Antibodies Anti-protein A (P-3775; Sigma-Aldrich), anti-GFP (11814460001; Roche), anti-Tor1 (sc-11900; Santa Cruz Biotechnology), anti-PGK (459250; Thermo Fisher Scientific), rabbit anti-Goat IgG HRP (anti-TAP) (ab6741; abcam), goat anti-mouse IgG, human ads-HRP (1030–05; Southern Bio Tech), anti-rabbit IgG HRP-linked antibody (7074S; Cell signaling), anti-Atg13 (a gift from Dr. Yoshinori Ohsumi, Tokyo Institute of Technology), and anti-HA (901501; BioLegend) antibodies were used for western blotting. .. Immunoprecipitation experiments Cells were resuspended in TAP-A buffer (50 mM Tris-HCl (pH 8.0) (Wako), 150 mM NaCl (Wako), 10% glycerol (Wako), 1 mM DTT (Wako), 1 mM EDTA (Sigma) supplemented with Complete EDTA-free protease inhibitor cocktail (Roche), 1 mM PMSF (Wako), 1 μM microcystin-LR (Wako) and PhosSTOP (Roche), and lysed using a FastPrep instrument (MP-Biomedicals) and zirconia beads.

    Article Title: Optimization of Formulations Consisting of Layered Double Hydroxide Nanoparticles and Small Interfering RNA for Efficient Knockdown of the Target Gene
    Article Snippet: PD-L1 RabMab antibodies (ab205921) (Abcam, USA; at dilution of 1:800–1000) were applied overnight at 4 °C. .. The membrane was washed with TBST and then incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (goat antirabbit IgG H & L (HRP), ab97051) (Abcam, USA; at dilution 1:5000) for 2 h. After washing, protein bands were visualized using Clarity western ECL substrate (Bio-Rad) and analyzed using ImageJ v1.40 software. .. Data were analyzed by one-way analysis of variance, followed by multiple comparisons using Tukey’s test within GraphPad Prism 6 software.

    Article Title: Gtr/Ego-independent TORC1 activation is achieved through a glutamine-sensitive interaction with Pib2 on the vacuolar membrane
    Article Snippet: Each sample was mixed with 50 μl of 4×loading buffer (800 mM Tris-Cl (pH 6.8) (Sigma), 6% SDS (nacalai tesque), 400 mM dithiothreitol (Wako), 8 M urea (Wako), 0.04% bromophenol blue(Sigma)) and subjected to SDS-PAGE and western blot analysis. .. Anti-protein A (P-3775; Sigma-Aldrich), anti-GFP (11814460001; Roche), anti-Tor1 (sc-11900; Santa Cruz Biotechnology), anti-PGK (459250; Thermo Fisher Scientific), rabbit anti-Goat IgG HRP (anti-TAP) (ab6741; abcam), goat anti-mouse IgG, human ads-HRP (1030–05; Southern Bio Tech), anti-rabbit IgG HRP-linked antibody (7074S; Cell signaling), anti-Atg13 (a gift from Dr. Yoshinori Ohsumi, Tokyo Institute of Technology), and anti-HA (901501; BioLegend) antibodies were used for western blotting. .. Cells were resuspended in TAP-A buffer (50 mM Tris-HCl (pH 8.0) (Wako), 150 mM NaCl (Wako), 10% glycerol (Wako), 1 mM DTT (Wako), 1 mM EDTA (Sigma) supplemented with Complete EDTA-free protease inhibitor cocktail (Roche), 1 mM PMSF (Wako), 1 μM microcystin-LR (Wako) and PhosSTOP (Roche), and lysed using a FastPrep instrument (MP-Biomedicals) and zirconia beads.

    Software:

    Article Title: Optimization of Formulations Consisting of Layered Double Hydroxide Nanoparticles and Small Interfering RNA for Efficient Knockdown of the Target Gene
    Article Snippet: PD-L1 RabMab antibodies (ab205921) (Abcam, USA; at dilution of 1:800–1000) were applied overnight at 4 °C. .. The membrane was washed with TBST and then incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (goat antirabbit IgG H & L (HRP), ab97051) (Abcam, USA; at dilution 1:5000) for 2 h. After washing, protein bands were visualized using Clarity western ECL substrate (Bio-Rad) and analyzed using ImageJ v1.40 software. .. Data were analyzed by one-way analysis of variance, followed by multiple comparisons using Tukey’s test within GraphPad Prism 6 software.

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  • 99
    Abcam monoclonal mouse antibody anti parkin
    RTP801 is elevated in <t>parkin</t> knockout <t>mouse</t> brains. Whole-brain lysates from five 8-month-old PaKO mice and five wild-type littermates (WT) were analyzed by western immunoblotting. Membranes were incubated with <t>anti-RTP801</t> and anti-parkin antibodies, and subsequently reprobed with anti- α -actin <t>antibody</t> as a loading control. Representative immunoblots are shown along with the densitometries representing values as mean ± S.E.M. of at least three independent membranes including all the samples. Student's t -test with Welch's correction, * P
    Monoclonal Mouse Antibody Anti Parkin, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal mouse antibody anti parkin/product/Abcam
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal mouse antibody anti parkin - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    96
    Abcam biotinylated secondary antibody
    Toxoplasma positive immunohistochemistry staining of cat with labeled-(Strept) <t>avidin-biotin</t> method: (a) Necrosis is present in kidney and (b) ileum (arrow) (brown color is indicating the positive result of immunohistochemistry) (scale bar=30 µm).
    Biotinylated Secondary Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated secondary antibody/product/Abcam
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary antibody - by Bioz Stars, 2021-03
    96/100 stars
      Buy from Supplier

    96
    Abcam rabbit anti cd4
    Double IHC staining in HLRCC. <t>CD4</t> + lymphocytes in red color, CD8 + lymphocytes in brown color. The peritumoral distribution of immune cells is seen, with not immune cells within the tumor areas. Magnification: A: 20x, B:40x.
    Rabbit Anti Cd4, supplied by Abcam, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti cd4/product/Abcam
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti cd4 - by Bioz Stars, 2021-03
    96/100 stars
      Buy from Supplier

    Image Search Results


    RTP801 is elevated in parkin knockout mouse brains. Whole-brain lysates from five 8-month-old PaKO mice and five wild-type littermates (WT) were analyzed by western immunoblotting. Membranes were incubated with anti-RTP801 and anti-parkin antibodies, and subsequently reprobed with anti- α -actin antibody as a loading control. Representative immunoblots are shown along with the densitometries representing values as mean ± S.E.M. of at least three independent membranes including all the samples. Student's t -test with Welch's correction, * P

    Journal: Cell Death & Disease

    Article Title: Parkin loss of function contributes to RTP801 elevation and neurodegeneration in Parkinson's disease

    doi: 10.1038/cddis.2014.333

    Figure Lengend Snippet: RTP801 is elevated in parkin knockout mouse brains. Whole-brain lysates from five 8-month-old PaKO mice and five wild-type littermates (WT) were analyzed by western immunoblotting. Membranes were incubated with anti-RTP801 and anti-parkin antibodies, and subsequently reprobed with anti- α -actin antibody as a loading control. Representative immunoblots are shown along with the densitometries representing values as mean ± S.E.M. of at least three independent membranes including all the samples. Student's t -test with Welch's correction, * P

    Article Snippet: Monoclonal mouse antibody anti-parkin used for WB and immunohistochemistry was purchased from Abcam (Cambridge, UK).

    Techniques: Knock-Out, Mouse Assay, Western Blot, Incubation

    RTP801 is poly-ubiquitinated by parkin E3 ligase and degraded by the proteasome. ( a ) RTP801 is degraded by the proteasome. HEK293 cells were exposed for 4 h to 1 μ M epoxomycin or 50 μ M chloroquine, and cell lysates were analyzed by western immunoblotting for RTP801 and α -actin (loading control). ut = untreated. ( b ) RTP801 is poly-ubiquitinated by parkin prior to proteasomal degradation. HEK293 cells were co-transfected with pCMS-eGFP RTP801, HA-ubiquitin and one of the pRK5-myc constructs, either empty or containing parkin WT or parkin ΔR2. Twenty-four hours later, cultures were exposed to epoxomycin for 3 h prior to harvesting. RTP801 was immunoprecipitated (IP) and immunocomplexes along with whole-cell lysates were analyzed by western immunoblotting (IB). Membranes were probed for HA, myc and α -actin as a loading control. Membranes were reprobed with anti-RTP801 antibody to confirm that RTP801 was immunoprecipitated. Non-specific mouse immunoglobulins were used as negative controls (m IgG). (HMW Ub-RTP801, high molecular weight ubiquitinated RTP801) ( c ) Parkin ubiquitinates RTP801 in a cell-free in vitro system. Recombinant parkin E3 ligase (active full-length), truncated parkin (inactive), GST-RTP801, and UbcH7 E2 enzyme, were mixed and incubated as indicated along with biotinylated ubiquitin, E1 enzyme, and ATP. RTP801 was immunoprecipitated, and immunocomplexes resolved in a western blot. The membrane was incubated with Avidin/Biotin and then incubated with chemiluminiscent peroxidase substrate solution (upper panel). The same membrane was reprobed with an anti-RTP801 antibody (lower panel). (HMW Ub-RTP801, high molecular weight ubiquitinated RTP801) ( d ) Ectopic parkin physically interacts with ectopic RTP801. HEK293 cells were co-transfected either with the pCMS-eGFP and pEGFP-C2 empty vectors or pCMS-eGFP RTP801 along with pEGFP-C2-parkin. Twenty-four hours later, cultures were treated with the cross-linking agent DSP for 2 h at 4°C, prior to harvesting. After RTP801 immunoprecipitation, the samples were analyzed by western immunoblotting for parkin to detect the interaction, and for RTP801 as an IP control. ( e and f ) Reciprocal co-immunoprecipitation indicates interaction of endogenous parkin and RTP801 in cells. Neuronal PC12 cells were treated with or without MG132 for 5 h as indicated, lysed and subjected to immunoprecipitation (IP) with control antibody (GFP), and either ( e ) anti-parkin or ( f ) anti-RTP801 antibodies. Immunoprecipitates were analyzed by western immunoblotting with anti-parkin and anti-RTP801 as indicated. In ( e ) all samples were analyzed on the same blot, but irrelevant intervening lanes were removed. In the right panel ( f ), the membrane was probed with a light chain-specific secondary antibody to diminish overlap of signal with co-immunoprecipitated RTP801. In each panel ( a , b , c , d , e and f ), a representative western blot is shown from a pool of at least two independent experiments. Specific bands are pointed out by arrows

    Journal: Cell Death & Disease

    Article Title: Parkin loss of function contributes to RTP801 elevation and neurodegeneration in Parkinson's disease

    doi: 10.1038/cddis.2014.333

    Figure Lengend Snippet: RTP801 is poly-ubiquitinated by parkin E3 ligase and degraded by the proteasome. ( a ) RTP801 is degraded by the proteasome. HEK293 cells were exposed for 4 h to 1 μ M epoxomycin or 50 μ M chloroquine, and cell lysates were analyzed by western immunoblotting for RTP801 and α -actin (loading control). ut = untreated. ( b ) RTP801 is poly-ubiquitinated by parkin prior to proteasomal degradation. HEK293 cells were co-transfected with pCMS-eGFP RTP801, HA-ubiquitin and one of the pRK5-myc constructs, either empty or containing parkin WT or parkin ΔR2. Twenty-four hours later, cultures were exposed to epoxomycin for 3 h prior to harvesting. RTP801 was immunoprecipitated (IP) and immunocomplexes along with whole-cell lysates were analyzed by western immunoblotting (IB). Membranes were probed for HA, myc and α -actin as a loading control. Membranes were reprobed with anti-RTP801 antibody to confirm that RTP801 was immunoprecipitated. Non-specific mouse immunoglobulins were used as negative controls (m IgG). (HMW Ub-RTP801, high molecular weight ubiquitinated RTP801) ( c ) Parkin ubiquitinates RTP801 in a cell-free in vitro system. Recombinant parkin E3 ligase (active full-length), truncated parkin (inactive), GST-RTP801, and UbcH7 E2 enzyme, were mixed and incubated as indicated along with biotinylated ubiquitin, E1 enzyme, and ATP. RTP801 was immunoprecipitated, and immunocomplexes resolved in a western blot. The membrane was incubated with Avidin/Biotin and then incubated with chemiluminiscent peroxidase substrate solution (upper panel). The same membrane was reprobed with an anti-RTP801 antibody (lower panel). (HMW Ub-RTP801, high molecular weight ubiquitinated RTP801) ( d ) Ectopic parkin physically interacts with ectopic RTP801. HEK293 cells were co-transfected either with the pCMS-eGFP and pEGFP-C2 empty vectors or pCMS-eGFP RTP801 along with pEGFP-C2-parkin. Twenty-four hours later, cultures were treated with the cross-linking agent DSP for 2 h at 4°C, prior to harvesting. After RTP801 immunoprecipitation, the samples were analyzed by western immunoblotting for parkin to detect the interaction, and for RTP801 as an IP control. ( e and f ) Reciprocal co-immunoprecipitation indicates interaction of endogenous parkin and RTP801 in cells. Neuronal PC12 cells were treated with or without MG132 for 5 h as indicated, lysed and subjected to immunoprecipitation (IP) with control antibody (GFP), and either ( e ) anti-parkin or ( f ) anti-RTP801 antibodies. Immunoprecipitates were analyzed by western immunoblotting with anti-parkin and anti-RTP801 as indicated. In ( e ) all samples were analyzed on the same blot, but irrelevant intervening lanes were removed. In the right panel ( f ), the membrane was probed with a light chain-specific secondary antibody to diminish overlap of signal with co-immunoprecipitated RTP801. In each panel ( a , b , c , d , e and f ), a representative western blot is shown from a pool of at least two independent experiments. Specific bands are pointed out by arrows

    Article Snippet: Monoclonal mouse antibody anti-parkin used for WB and immunohistochemistry was purchased from Abcam (Cambridge, UK).

    Techniques: Western Blot, Transfection, Construct, Immunoprecipitation, Molecular Weight, In Vitro, Recombinant, Incubation, Avidin-Biotin Assay

    Toxoplasma positive immunohistochemistry staining of cat with labeled-(Strept) avidin-biotin method: (a) Necrosis is present in kidney and (b) ileum (arrow) (brown color is indicating the positive result of immunohistochemistry) (scale bar=30 µm).

    Journal: Veterinary World

    Article Title: Detection of Toxoplasma gondii in cat’s internal organs by immunohistochemistry methods labeled with-[strept] avidin-biotin

    doi: 10.14202/vetworld.2017.1035-1039

    Figure Lengend Snippet: Toxoplasma positive immunohistochemistry staining of cat with labeled-(Strept) avidin-biotin method: (a) Necrosis is present in kidney and (b) ileum (arrow) (brown color is indicating the positive result of immunohistochemistry) (scale bar=30 µm).

    Article Snippet: Slides were incubated with primary polyclonal anti-T. gondii antibody (rabbit) primary antibody (Abcam, USA) diluted 1/100 at 37°C for 32 min and biotinylated secondary antibody (Abcam, USA).

    Techniques: Immunohistochemistry, Staining, Labeling, Avidin-Biotin Assay

    Binding interactions between BMP2 and heparin are reversed by HS antagonism. (A) Association–dissociation curves of rhBMP2 injected on a streptavidin chip coated with biotinylated heparin. Binding constants were calculated and are shown in

    Journal: Developmental biology

    Article Title: Perichondrium phenotype and border function are regulated by Ext1 and heparan sulfate in developing long bones: A mechanism likely deranged in Hereditary Multiple Exostoses

    doi: 10.1016/j.ydbio.2013.02.008

    Figure Lengend Snippet: Binding interactions between BMP2 and heparin are reversed by HS antagonism. (A) Association–dissociation curves of rhBMP2 injected on a streptavidin chip coated with biotinylated heparin. Binding constants were calculated and are shown in

    Article Snippet: Following rinsing, sections were then incubated with biotinylated anti-rabbit secondary antibody and the signal was visualized using a HRP/DAB detection IHC kit according to the manufacturer’s instructions (Abcam).

    Techniques: Binding Assay, Injection, Chromatin Immunoprecipitation

    Double IHC staining in HLRCC. CD4 + lymphocytes in red color, CD8 + lymphocytes in brown color. The peritumoral distribution of immune cells is seen, with not immune cells within the tumor areas. Magnification: A: 20x, B:40x.

    Journal: Journal of Cancer

    Article Title: Determination of the Expression of PD-L1 in the Morphologic Spectrum of Renal Cell Carcinoma

    doi: 10.7150/jca.35738

    Figure Lengend Snippet: Double IHC staining in HLRCC. CD4 + lymphocytes in red color, CD8 + lymphocytes in brown color. The peritumoral distribution of immune cells is seen, with not immune cells within the tumor areas. Magnification: A: 20x, B:40x.

    Article Snippet: For the secondary antibody, sections were incubated with Rabbit Anti-CD4 (EPR6855; Abcam, at 1:500dil) followed by avidin-biotin complex for 15 minutes at room temperature and developed alkaline phosphatase using a kit from Vector Laboratories.

    Techniques: Immunohistochemistry, Staining