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Dianova biotinylated secondary antibodies
Biotinylated Secondary Antibodies, supplied by Dianova, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotinylated secondary antibodies/product/Dianova
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
biotinylated secondary antibodies - by Bioz Stars, 2021-03
86/100 stars

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Incubation:

Article Title: Selective increases in inter-individual variability in response to environmental enrichment in female mice
Article Snippet: Primary antibodies were applied overnight at 4°C as follows: monoclonal rat anti-BrdU (1:500, Serotec), rabbit anti-Ki67 (Novocastra, 1:500), and rabbit anti-Synaptoporin (Synaptic Systems, 1:500). .. Sections were incubated with biotinylated secondary antibodies for 2 hr at room temperature (1:500, Dianova). ..

Article Title: Simultaneous effects on parvalbumin-positive interneuron and dopaminergic system development in a transgenic rat model for sporadic schizophrenia
Article Snippet: Anti-tyrosine-hydroxylase-antibody (1:100, mouse-monoclonal, Millipore, Billerica, MA, USA, MAB318), anti-GAD67-antibody (1:500, mouse-monoclonal, Millipore, Billerica, MA, USA, MAB5406), anti-parvalbumin-antibody (1:10.000, mouse-monoclonal, Sigma-Aldrich, St. Louis, MO, USA, P-3171) and anti-calbindin-antibody (1:10.000, mouse-monoclonal, Sigma-Aldrich, C-9848) were used as primary antibodies. .. Rinsing in PBS (pH 7.4) was carried out three times for 30 min. Biotinylated secondary anti-mouse antibodies (1:500, Dianova, Hamburg, Germany, 115-065-166) diluted in PBS (pH 7.4) were used as secondary antibodies and sections incubated for 24 h at 4 °C, then rinsed in PBS (pH 7.4) three times for 15 min. .. Sections were incubated with ABC reagent for 30 min at room temperature (ABC elite kit, 1:300, Vector laboratories, Burlingame, CA, USA, PK-6100) and washed out for 15 min using PBS (pH 7.4), followed by incubation with trisamine buffer twice for 15 min (pH 8.1, 0.05 M, Trizma® base, Sigma-Aldrich, T1503).

Article Title: Bid Expression Network Controls Neuronal Cell Fate During Avian Ciliary Ganglion Development
Article Snippet: .. Sections were de-paraffinized and heated in citrate buffer for improved antigen retrieval and further incubated with anti-islet-1/2 (40.2D6) 1:50; anti-gag (AMV3C2, 1:200; both from Developmental Studies Hybridoma Bank, University of Iowa), anti-BrdU (Sigma; 1:1000); anti-active-caspase-3 (R & D; 1:500) and visualized using biotinylated secondary antibodies (donkey-anti-mouse; -anti-rabbit; -anti-rat; 1:100; Dianova, Hamburg, Germany) and diaminobenzidine (Vectastain Peroxidase ABC-kit 6100, Vector Laboratories, CA, United States). .. Sections were de-paraffinized and in situ hybridization and preparation of digoxigenin-labeled probes for chicken BID and BCL-XL were performed as described previously ( ).

Article Title: Trefoil Factor 3 (TFF3) Is Involved in Cell Migration for Skeletal Repair
Article Snippet: For immunohistochemistry with an antiserum against TFF3 [ ], deparaffinized sections were pretreated with 0.05% trypsin for 60 min. .. The sections were then left to react with anti-human TFF3, serum (anti-rTFF3-1) [ ] diluted 1:300, followed by incubation with biotinylated secondary antibodies (Dianova, Hamburg, Germany). .. Negative control sections were incubated with isotype normal mouse IgG (Santa Cruz Biotechnology).

Avidin-Biotin Assay:

Article Title: COX1 and COX2 Expression in Non-neuronal Cellular Compartments of the Rhesus Macaque Brain during Lentiviral Infection
Article Snippet: Immunohistochemistry (IHC) was carried out on deparaffinized brain tissue sections (7 µm) and incubated for 15 min at 92–95°C in citrate buffer (pH 6.0) in a pressure cooker for antigen retrieval, as previously described ( ). .. Visualization of bound primary antibodies was performed using biotinylated secondary antibodies from donkey (Dianova, Hamburg, Germany; 1/200), standard avidin-biotin-peroxidase techniques (Vectastain Elite ABC kit, Boehringer, Germany; 1/500), and finally with 3,3’-diaminobenzidine (Sigma, Deisenhofen, Germany) and ammonium nickel sulfate (Fluka, Buchs, Switzerland), resulting in a dark blue/black staining, or by indirect immunofluorescence with fluorochrome-conjugated secondary antibodies (Dianova; 1/200). .. For visualization of two different antigens in the same tissue section double immunofluorescence was done.

Staining:

Article Title: COX1 and COX2 Expression in Non-neuronal Cellular Compartments of the Rhesus Macaque Brain during Lentiviral Infection
Article Snippet: Immunohistochemistry (IHC) was carried out on deparaffinized brain tissue sections (7 µm) and incubated for 15 min at 92–95°C in citrate buffer (pH 6.0) in a pressure cooker for antigen retrieval, as previously described ( ). .. Visualization of bound primary antibodies was performed using biotinylated secondary antibodies from donkey (Dianova, Hamburg, Germany; 1/200), standard avidin-biotin-peroxidase techniques (Vectastain Elite ABC kit, Boehringer, Germany; 1/500), and finally with 3,3’-diaminobenzidine (Sigma, Deisenhofen, Germany) and ammonium nickel sulfate (Fluka, Buchs, Switzerland), resulting in a dark blue/black staining, or by indirect immunofluorescence with fluorochrome-conjugated secondary antibodies (Dianova; 1/200). .. For visualization of two different antigens in the same tissue section double immunofluorescence was done.

Immunofluorescence:

Article Title: COX1 and COX2 Expression in Non-neuronal Cellular Compartments of the Rhesus Macaque Brain during Lentiviral Infection
Article Snippet: Immunohistochemistry (IHC) was carried out on deparaffinized brain tissue sections (7 µm) and incubated for 15 min at 92–95°C in citrate buffer (pH 6.0) in a pressure cooker for antigen retrieval, as previously described ( ). .. Visualization of bound primary antibodies was performed using biotinylated secondary antibodies from donkey (Dianova, Hamburg, Germany; 1/200), standard avidin-biotin-peroxidase techniques (Vectastain Elite ABC kit, Boehringer, Germany; 1/500), and finally with 3,3’-diaminobenzidine (Sigma, Deisenhofen, Germany) and ammonium nickel sulfate (Fluka, Buchs, Switzerland), resulting in a dark blue/black staining, or by indirect immunofluorescence with fluorochrome-conjugated secondary antibodies (Dianova; 1/200). .. For visualization of two different antigens in the same tissue section double immunofluorescence was done.

Immunohistochemistry:

Article Title: Lack of Intestinal Epithelial Atg7 Affects Paneth Cell Granule Formation but Does Not Compromise Immune Homeostasis in the Gut
Article Snippet: Sections of paraffin embedded tissues were stained with H & E or combined staining methods with alcian blue, PAS, Elastica, and van Gieson to visualize tissue structures. .. Immunohistochemical analysis of cryosections was performed using Anti-CD11c antibodies (BD Pharmingen), Anti-lysozyme antibodies (Dianova), and Anti-myeloperoxidase antibodies (Abcam) as primary antibodies, biotinylated secondary antibodies (Dianova) and the TSA Cy3 system (PerkinElmer) as recommended by the manufacturer. .. Apoptotic cells were detected using In situ Cell Death Detection Kit Fluorescein (Roche) for TdT-mediated dUTP nick end labelling (TUNEL) according to manufacture recommendations.

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  • 86
    Dianova biotinylated secondary rabbit antibody
    Ang II promotes nephrin endocytosis. ( a ) Ang II induces the endocytosis of nephrin in HEK293T cells significantly in a time dependent fashion. The <t>biotinylated</t> nephrin fraction decreases the longer the Ang II exposure lasts (Kruskal-Wallis test: *n = 5, p = 0.04; **n = 5, p = 0.009). The AT1-receptor plasmid was cotransfected in all conditions to the AT1-receptor deficient HEK293T cells. ( b ) Nephrin endocytosis in murine podocytes is significantly enhanced by Ang II (*n = 5, p = 0.03). Biotinylated nephrin is significantly decreased in mouse podocytes when they are stimulated with Ang II. ( c ) Nephrin endocytosis in mice without and with Ang II stimulation and additional candesartan treatment. Biotinylated nephrin is significantly decreased in Ang II treated animals compared to control mice and restored to nearly control levels by additional candesartan treatment. (*Kruskal-Wallis test: n = 5, p = 0.02).
    Biotinylated Secondary Rabbit Antibody, supplied by Dianova, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated secondary rabbit antibody/product/Dianova
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary rabbit antibody - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Dianova biotinylated secondary mouse anti rat antibody
    Localization of cell types in the spleen. Mice were injected with 1 × 10 9 PKH-26-labeled (red) pRBC. Cryosections were incubated with the primary antibodies indicated and were developed using <t>biotinylated</t> secondary antibodies and streptavidin-Alexa 488 (green). Separate green and red images were taken by confocal laser scanning microscopy and merged.
    Biotinylated Secondary Mouse Anti Rat Antibody, supplied by Dianova, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated secondary mouse anti rat antibody/product/Dianova
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary mouse anti rat antibody - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Dianova biotinylated anti rabbit secondary antibody
    Localization of cell types in the spleen. Mice were injected with 1 × 10 9 PKH-26-labeled (red) pRBC. Cryosections were incubated with the primary antibodies indicated and were developed using <t>biotinylated</t> secondary antibodies and streptavidin-Alexa 488 (green). Separate green and red images were taken by confocal laser scanning microscopy and merged.
    Biotinylated Anti Rabbit Secondary Antibody, supplied by Dianova, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated anti rabbit secondary antibody/product/Dianova
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated anti rabbit secondary antibody - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Dianova biotinylated secondary antibodies
    Localization of cell types in the spleen. Mice were injected with 1 × 10 9 PKH-26-labeled (red) pRBC. Cryosections were incubated with the primary antibodies indicated and were developed using <t>biotinylated</t> secondary antibodies and streptavidin-Alexa 488 (green). Separate green and red images were taken by confocal laser scanning microscopy and merged.
    Biotinylated Secondary Antibodies, supplied by Dianova, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated secondary antibodies/product/Dianova
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary antibodies - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    Ang II promotes nephrin endocytosis. ( a ) Ang II induces the endocytosis of nephrin in HEK293T cells significantly in a time dependent fashion. The biotinylated nephrin fraction decreases the longer the Ang II exposure lasts (Kruskal-Wallis test: *n = 5, p = 0.04; **n = 5, p = 0.009). The AT1-receptor plasmid was cotransfected in all conditions to the AT1-receptor deficient HEK293T cells. ( b ) Nephrin endocytosis in murine podocytes is significantly enhanced by Ang II (*n = 5, p = 0.03). Biotinylated nephrin is significantly decreased in mouse podocytes when they are stimulated with Ang II. ( c ) Nephrin endocytosis in mice without and with Ang II stimulation and additional candesartan treatment. Biotinylated nephrin is significantly decreased in Ang II treated animals compared to control mice and restored to nearly control levels by additional candesartan treatment. (*Kruskal-Wallis test: n = 5, p = 0.02).

    Journal: Scientific Reports

    Article Title: Angiotensin II increases glomerular permeability by β-arrestin mediated nephrin endocytosis

    doi: 10.1038/srep39513

    Figure Lengend Snippet: Ang II promotes nephrin endocytosis. ( a ) Ang II induces the endocytosis of nephrin in HEK293T cells significantly in a time dependent fashion. The biotinylated nephrin fraction decreases the longer the Ang II exposure lasts (Kruskal-Wallis test: *n = 5, p = 0.04; **n = 5, p = 0.009). The AT1-receptor plasmid was cotransfected in all conditions to the AT1-receptor deficient HEK293T cells. ( b ) Nephrin endocytosis in murine podocytes is significantly enhanced by Ang II (*n = 5, p = 0.03). Biotinylated nephrin is significantly decreased in mouse podocytes when they are stimulated with Ang II. ( c ) Nephrin endocytosis in mice without and with Ang II stimulation and additional candesartan treatment. Biotinylated nephrin is significantly decreased in Ang II treated animals compared to control mice and restored to nearly control levels by additional candesartan treatment. (*Kruskal-Wallis test: n = 5, p = 0.02).

    Article Snippet: Anti-EEA-1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA 1:100) was stained with a biotinylated secondary rabbit antibody (Dianova, Hamburg, Germany 1:1000) and visualized with Streptavidin Cy3 (Dianova, Hamburg, Germany 1:1000).

    Techniques: Plasmid Preparation, Mouse Assay

    Localization of cell types in the spleen. Mice were injected with 1 × 10 9 PKH-26-labeled (red) pRBC. Cryosections were incubated with the primary antibodies indicated and were developed using biotinylated secondary antibodies and streptavidin-Alexa 488 (green). Separate green and red images were taken by confocal laser scanning microscopy and merged.

    Journal: Infection and Immunity

    Article Title: Massive Destruction of Malaria-Parasitized Red Blood Cells despite Spleen Closure

    doi: 10.1128/IAI.73.10.6390-6398.2005

    Figure Lengend Snippet: Localization of cell types in the spleen. Mice were injected with 1 × 10 9 PKH-26-labeled (red) pRBC. Cryosections were incubated with the primary antibodies indicated and were developed using biotinylated secondary antibodies and streptavidin-Alexa 488 (green). Separate green and red images were taken by confocal laser scanning microscopy and merged.

    Article Snippet: Signal amplification was performed using a biotinylated secondary mouse anti-rat antibody (Dianova, Hamburg, Germany) and Alexa fluor 488-coupled streptavidin (Molecular Probes).

    Techniques: Mouse Assay, Injection, Labeling, Incubation, Confocal Laser Scanning Microscopy