Structured Review

Agilent technologies biotinylated secondary antibodies
Labeling for apoptotic nuclei. End labeling with <t>biotinylated</t> nucleotides was visualized with a diaminobenzidine chromagen with a methylene green counterstain. End labeling occurred after ( a ) TDP-43 injections, but not after ( b ) control injections ( c ) higher magnification of end labeling as in a . Interval of 2 weeks and equal vector doses of 3 × 10 10 vector genomes. a,b , bar = 34 µm; c , bar = 21 µm.
Biotinylated Secondary Antibodies, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 337 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotinylated secondary antibodies/product/Agilent technologies
Average 92 stars, based on 337 article reviews
Price from $9.99 to $1999.99
biotinylated secondary antibodies - by Bioz Stars, 2021-01
92/100 stars

Images

1) Product Images from "Mimicking Aspects of Frontotemporal Lobar Degeneration and Lou Gehrig's Disease in Rats via TDP-43 Overexpression"

Article Title: Mimicking Aspects of Frontotemporal Lobar Degeneration and Lou Gehrig's Disease in Rats via TDP-43 Overexpression

Journal: Molecular Therapy: the Journal of the American Society of Gene Therapy

doi: 10.1038/mt.2009.3

Labeling for apoptotic nuclei. End labeling with biotinylated nucleotides was visualized with a diaminobenzidine chromagen with a methylene green counterstain. End labeling occurred after ( a ) TDP-43 injections, but not after ( b ) control injections ( c ) higher magnification of end labeling as in a . Interval of 2 weeks and equal vector doses of 3 × 10 10 vector genomes. a,b , bar = 34 µm; c , bar = 21 µm.
Figure Legend Snippet: Labeling for apoptotic nuclei. End labeling with biotinylated nucleotides was visualized with a diaminobenzidine chromagen with a methylene green counterstain. End labeling occurred after ( a ) TDP-43 injections, but not after ( b ) control injections ( c ) higher magnification of end labeling as in a . Interval of 2 weeks and equal vector doses of 3 × 10 10 vector genomes. a,b , bar = 34 µm; c , bar = 21 µm.

Techniques Used: Labeling, End Labeling, Plasmid Preparation

2) Product Images from "Inflammation, oxidative stress and apoptosis cascade implications in bisphenol A‐induced liver fibrosis in male rats"

Article Title: Inflammation, oxidative stress and apoptosis cascade implications in bisphenol A‐induced liver fibrosis in male rats

Journal: International Journal of Experimental Pathology

doi: 10.1111/iep.12207

Representative photomicrographs of liver sections illustrating expression of BCL 2 antigen by immunohistochemical staining (avidin–biotin–peroxidase stain with H counter stain, magnification ×400). (A) A liver section of an adult
Figure Legend Snippet: Representative photomicrographs of liver sections illustrating expression of BCL 2 antigen by immunohistochemical staining (avidin–biotin–peroxidase stain with H counter stain, magnification ×400). (A) A liver section of an adult

Techniques Used: Expressing, Immunohistochemistry, Staining, Avidin-Biotin Assay

3) Product Images from "Expression of nitric oxide synthase isoforms in pregnant human myometrium"

Article Title: Expression of nitric oxide synthase isoforms in pregnant human myometrium

Journal: The Journal of Physiology

doi: 10.1111/j.1469-7793.1999.00705.x

Immunohistochemical staining of sections of human pregnant myometrial tissue with polyclonal anti-NOS antibodies Representative sections are shown from preterm and term non-labouring myometrium and from tissue of women in active labour at term. Cryostat sections 5 μm thick were incubated with polyclonal antibodies raised against eNOS, iNOS and nNOS. Negative controls were incubated with non-immune serum. Staining was by avidin-biotin-peroxidase.
Figure Legend Snippet: Immunohistochemical staining of sections of human pregnant myometrial tissue with polyclonal anti-NOS antibodies Representative sections are shown from preterm and term non-labouring myometrium and from tissue of women in active labour at term. Cryostat sections 5 μm thick were incubated with polyclonal antibodies raised against eNOS, iNOS and nNOS. Negative controls were incubated with non-immune serum. Staining was by avidin-biotin-peroxidase.

Techniques Used: Immunohistochemistry, Staining, Incubation, Avidin-Biotin Assay

Immunohistochemical staining of sections of human pregnant myometrial tissue with monoclonal anti-NOS antibodies Representative sections are shown of term non-labouring tissue. Cryostat sections 5 μm thick were incubated with monoclonal antibodies raised against eNOS and iNOS. Negative controls were incubated with non-immune mouse IgG. Staining was by avidin-biotin-peroxidase.
Figure Legend Snippet: Immunohistochemical staining of sections of human pregnant myometrial tissue with monoclonal anti-NOS antibodies Representative sections are shown of term non-labouring tissue. Cryostat sections 5 μm thick were incubated with monoclonal antibodies raised against eNOS and iNOS. Negative controls were incubated with non-immune mouse IgG. Staining was by avidin-biotin-peroxidase.

Techniques Used: Immunohistochemistry, Staining, Incubation, Avidin-Biotin Assay

4) Product Images from "Formation of New Vasa Vasorum in Vasculitis "

Article Title: Formation of New Vasa Vasorum in Vasculitis

Journal: The American Journal of Pathology

doi:

Production of vascular endothelial growth factor (VEGF) in temporal arteries with GCA. Tissue sections from temporal arteries were stained with anti-VEGF and anti-CD68 Abs. Anti-CD68 was visualized by using an FITC-labeled secondary antibody, anti-VEGF was developed by using a secondary biotinylated antibody, streptavidine-alkaline phosphatase and vector red. Stained sections were scanned by confocal microscopy and composite pictures were generated. Stainings for VEGF ( A ) and CD68 ( B ) are shown at an original magnification of ×100. VEGF production in the arterial wall was unevenly distributed with VEGF + cells (red) accumulating at the media-intima junction. A small population of VEGF-producing cells was detected along the external elastic lamina. Cell nuclei are stained blue. More than 90% of the VEGF-producing cells expressed the CD68 marker. C : Strong expression of VEGF was typical for multinucleated giant cells. Blue, cell nuclei; red, VEGF; green, CD68; yellow, co-expression of VEGF and CD68. Original magnification, ×630. D : Control stainings without primary antibodies only showed minimal autofluorescence.
Figure Legend Snippet: Production of vascular endothelial growth factor (VEGF) in temporal arteries with GCA. Tissue sections from temporal arteries were stained with anti-VEGF and anti-CD68 Abs. Anti-CD68 was visualized by using an FITC-labeled secondary antibody, anti-VEGF was developed by using a secondary biotinylated antibody, streptavidine-alkaline phosphatase and vector red. Stained sections were scanned by confocal microscopy and composite pictures were generated. Stainings for VEGF ( A ) and CD68 ( B ) are shown at an original magnification of ×100. VEGF production in the arterial wall was unevenly distributed with VEGF + cells (red) accumulating at the media-intima junction. A small population of VEGF-producing cells was detected along the external elastic lamina. Cell nuclei are stained blue. More than 90% of the VEGF-producing cells expressed the CD68 marker. C : Strong expression of VEGF was typical for multinucleated giant cells. Blue, cell nuclei; red, VEGF; green, CD68; yellow, co-expression of VEGF and CD68. Original magnification, ×630. D : Control stainings without primary antibodies only showed minimal autofluorescence.

Techniques Used: Staining, Labeling, Plasmid Preparation, Confocal Microscopy, Generated, Marker, Expressing

5) Product Images from "?-synuclein induced synapse damage is enhanced by amyloid-?1-42"

Article Title: ?-synuclein induced synapse damage is enhanced by amyloid-?1-42

Journal: Molecular Neurodegeneration

doi: 10.1186/1750-1326-5-55

αSN did not affect the accumulation of Aβ 1-42 in synapses . (A) The amount of biotinylated-Aβ 1-42 found in synaptosomes derived from cortical neurons incubated for 1 hour with 100 nM Aβ 1-42 (□) or 100 nM Aβ 1-42 that had been pre-mixed with 500 nM αSN (■). Values shown are the mean amount of Aβ 1-42 expressed as a % of the amount added ± SD, n = 12. (B) Immunoblot showing the amount of αSN in synaptosomes collected from cortical neurons incubated with 200 nM αSN alone or with 200 nM αSN pre-mixed with Aβ 1-42 (50:1).
Figure Legend Snippet: αSN did not affect the accumulation of Aβ 1-42 in synapses . (A) The amount of biotinylated-Aβ 1-42 found in synaptosomes derived from cortical neurons incubated for 1 hour with 100 nM Aβ 1-42 (□) or 100 nM Aβ 1-42 that had been pre-mixed with 500 nM αSN (■). Values shown are the mean amount of Aβ 1-42 expressed as a % of the amount added ± SD, n = 12. (B) Immunoblot showing the amount of αSN in synaptosomes collected from cortical neurons incubated with 200 nM αSN alone or with 200 nM αSN pre-mixed with Aβ 1-42 (50:1).

Techniques Used: Derivative Assay, Incubation

Related Articles

Avidin-Biotin Assay:

Article Title: Expression of nitric oxide synthase isoforms in pregnant human myometrium
Article Snippet: .. Normal sera, biotinylated secondary antibodies and avidin- biotin-peroxidase complex (ABC) were from Dako Ltd (High Wycombe, UK); ADP-sepharose, RNAse inhibitor, enhanced chemiluminescence reagents (ECL+plus) from Amersham Pharmacia Biotech; proteinase K, M-MLV reverse transcriptase, MCDB 131 medium, and Dulbecco's modified Eagle's medium (DMEM) from Gibco Life Technologies; Biotaq polymerase from Biotaq Bioline (London, UK); Nylon transfer membrane from Micron Separations Inc. (Westborough, MA, USA); lipopolysaccharide (LPS) from Difco (Surrey, UK); recombinant human interleukin-1 beta (IL-1β), interferon gamma (IFN-γ), and tumour necrosis factor alpha (TNF-α) from R & D systems (Abingdon, UK); recombinant human nNOS was kindly provided by Dr Valentina Riveros Moreno (King's College London). ..

Article Title: circCELSR1 (hsa_circ_0063809) Contributes to Paclitaxel Resistance of Ovarian Cancer Cells by Regulating FOXR2 Expression via miR-1252
Article Snippet: .. After that, the sections were incubated with a Ki-67 antibody (dilution 1:200; Cell Signaling Technology) overnight at 4°C and a biotinylated secondary antibody for another 30 min. An avidin-biotin-peroxidase complex (Dako LSAB2 System; Dako, Produktionsvej, Denmark) was added, and the color was developed using diaminobenzidine (DAB). .. The sections were viewed under a BX51 system microscope (Olympus, Tokyo, Japan).

Incubation:

Article Title: Biotin-avidin mediates the binding of adipose-derived stem cells to a porous β-tricalcium phosphate scaffold: Mandibular regeneration
Article Snippet: .. After rinsing in PBS three times, the tissue sections were incubated with biotinylated secondary antibody (1:500; Z0420; DAKO, Glostrup, Denmark) at room temperature for 1 h. Peroxidase activity was visualized using 0.05% diaminobenzidine and 0.03% H2O2 in PBS. .. Subsequently, the tissue sections were counter-stained with hematoxylin for 2 min. At least 10 fields of vision under ×200 magnification were captured under brightfield view and images were subsequently analyzed and quantified using ImageJ software, version 1.47 (National Institutes of Health, Bethesda, MA, USA) ( ).

Article Title: circCELSR1 (hsa_circ_0063809) Contributes to Paclitaxel Resistance of Ovarian Cancer Cells by Regulating FOXR2 Expression via miR-1252
Article Snippet: .. After that, the sections were incubated with a Ki-67 antibody (dilution 1:200; Cell Signaling Technology) overnight at 4°C and a biotinylated secondary antibody for another 30 min. An avidin-biotin-peroxidase complex (Dako LSAB2 System; Dako, Produktionsvej, Denmark) was added, and the color was developed using diaminobenzidine (DAB). .. The sections were viewed under a BX51 system microscope (Olympus, Tokyo, Japan).

Article Title: Mimicking Aspects of Frontotemporal Lobar Degeneration and Lou Gehrig's Disease in Rats via TDP-43 Overexpression
Article Snippet: .. Biotinylated secondary antibodies for peroxidase staining were from DAKO Cytomation (1:2,000; Carpinteria, CA), incubated for 1 hour at room temperature. .. The sections were washed with PBS and labeled with horseradish peroxidase-conjugated Extravidin (1:2,000; Sigma, St Louis, MO) for 30 minutes at room temperature.

Article Title: CD147 is a Novel Interaction Partner of Integrin αMβ2 Mediating Leukocyte and Platelet Adhesion
Article Snippet: .. Recombinant CD147 was added in increasing concentrations (0–20 µg/mL) for 1 h. After removing CD147 and gentle washing, the wells were incubated with an anti-CD147 antibody (mouse-anti CD147, Abcam, Cambridge, UK) followed by a biotinylated secondary antibody (polyclonal rabbit anti mouse, Dako, Glostrup, Denmark) and a streptavidin/HRP complex (Life technologies, Carlsbad, CA, USA). .. The binding was detected using 3,3′,5,5′-tetramethylbenzidine (Serva, Heidelberg, Germany).

Article Title: Inflammation, oxidative stress and apoptosis cascade implications in bisphenol A‐induced liver fibrosis in male rats
Article Snippet: .. Biotinylated secondary antibodies (DakoCytomation, Carpinteria, CA, USA) directed against immunoglobulin were then added and incubated for 15 min. .. Protein expression level was evaluated using a streptavidin–biotin–peroxidase kit.

Activity Assay:

Article Title: Biotin-avidin mediates the binding of adipose-derived stem cells to a porous β-tricalcium phosphate scaffold: Mandibular regeneration
Article Snippet: .. After rinsing in PBS three times, the tissue sections were incubated with biotinylated secondary antibody (1:500; Z0420; DAKO, Glostrup, Denmark) at room temperature for 1 h. Peroxidase activity was visualized using 0.05% diaminobenzidine and 0.03% H2O2 in PBS. .. Subsequently, the tissue sections were counter-stained with hematoxylin for 2 min. At least 10 fields of vision under ×200 magnification were captured under brightfield view and images were subsequently analyzed and quantified using ImageJ software, version 1.47 (National Institutes of Health, Bethesda, MA, USA) ( ).

Modification:

Article Title: Expression of nitric oxide synthase isoforms in pregnant human myometrium
Article Snippet: .. Normal sera, biotinylated secondary antibodies and avidin- biotin-peroxidase complex (ABC) were from Dako Ltd (High Wycombe, UK); ADP-sepharose, RNAse inhibitor, enhanced chemiluminescence reagents (ECL+plus) from Amersham Pharmacia Biotech; proteinase K, M-MLV reverse transcriptase, MCDB 131 medium, and Dulbecco's modified Eagle's medium (DMEM) from Gibco Life Technologies; Biotaq polymerase from Biotaq Bioline (London, UK); Nylon transfer membrane from Micron Separations Inc. (Westborough, MA, USA); lipopolysaccharide (LPS) from Difco (Surrey, UK); recombinant human interleukin-1 beta (IL-1β), interferon gamma (IFN-γ), and tumour necrosis factor alpha (TNF-α) from R & D systems (Abingdon, UK); recombinant human nNOS was kindly provided by Dr Valentina Riveros Moreno (King's College London). ..

Staining:

Article Title: Mimicking Aspects of Frontotemporal Lobar Degeneration and Lou Gehrig's Disease in Rats via TDP-43 Overexpression
Article Snippet: .. Biotinylated secondary antibodies for peroxidase staining were from DAKO Cytomation (1:2,000; Carpinteria, CA), incubated for 1 hour at room temperature. .. The sections were washed with PBS and labeled with horseradish peroxidase-conjugated Extravidin (1:2,000; Sigma, St Louis, MO) for 30 minutes at room temperature.

Recombinant:

Article Title: Expression of nitric oxide synthase isoforms in pregnant human myometrium
Article Snippet: .. Normal sera, biotinylated secondary antibodies and avidin- biotin-peroxidase complex (ABC) were from Dako Ltd (High Wycombe, UK); ADP-sepharose, RNAse inhibitor, enhanced chemiluminescence reagents (ECL+plus) from Amersham Pharmacia Biotech; proteinase K, M-MLV reverse transcriptase, MCDB 131 medium, and Dulbecco's modified Eagle's medium (DMEM) from Gibco Life Technologies; Biotaq polymerase from Biotaq Bioline (London, UK); Nylon transfer membrane from Micron Separations Inc. (Westborough, MA, USA); lipopolysaccharide (LPS) from Difco (Surrey, UK); recombinant human interleukin-1 beta (IL-1β), interferon gamma (IFN-γ), and tumour necrosis factor alpha (TNF-α) from R & D systems (Abingdon, UK); recombinant human nNOS was kindly provided by Dr Valentina Riveros Moreno (King's College London). ..

Article Title: CD147 is a Novel Interaction Partner of Integrin αMβ2 Mediating Leukocyte and Platelet Adhesion
Article Snippet: .. Recombinant CD147 was added in increasing concentrations (0–20 µg/mL) for 1 h. After removing CD147 and gentle washing, the wells were incubated with an anti-CD147 antibody (mouse-anti CD147, Abcam, Cambridge, UK) followed by a biotinylated secondary antibody (polyclonal rabbit anti mouse, Dako, Glostrup, Denmark) and a streptavidin/HRP complex (Life technologies, Carlsbad, CA, USA). .. The binding was detected using 3,3′,5,5′-tetramethylbenzidine (Serva, Heidelberg, Germany).

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    ge healthcare biotinylated secondary anti mouse
    Monoclonal antibody 101-1-1 recognizes Aβ −3–40 with high selectivity on capillary isoelectric focusing immunoassay. A series of synthetic Aβ peptides with different N-termini was separated by isoelectric focusing in microcapillaries, immobilized by a photochemical reaction, and probed with ( a ) mAb 6E10 (2 µg/mL) or ( b ) mAb 101-1-1 (2.1 µg/mL). Chemiluminescent detection was achieved with <t>biotinylated</t> goat-anti mouse IgG antibody in combination with streptavidin-HRP (streptavidin horseradish peroxase conjugate) and chemiluminescent substrate. The specific Aβ variants loaded in the different capillaries as single peptides or in mixtures are indicated. Aβ 1–40 , Aβ 2–40 , Aβ 3–40 , Aβ 4–40 , Aβ 5–40 , and Aβ −3–40 were loaded at a concentration of 100 ng/mL. The tested concentration of Aβ N3pE–40 and VKMDAEFRC-bovine serum albumin (BSA) was 200 ng/mL.
    Biotinylated Secondary Anti Mouse, supplied by ge healthcare, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated secondary anti mouse/product/ge healthcare
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary anti mouse - by Bioz Stars, 2021-01
    88/100 stars
      Buy from Supplier

    92
    Agilent technologies biotinylated secondary antibodies
    Monoclonal antibody 101-1-1 recognizes Aβ −3–40 with high selectivity on capillary isoelectric focusing immunoassay. A series of synthetic Aβ peptides with different N-termini was separated by isoelectric focusing in microcapillaries, immobilized by a photochemical reaction, and probed with ( a ) mAb 6E10 (2 µg/mL) or ( b ) mAb 101-1-1 (2.1 µg/mL). Chemiluminescent detection was achieved with <t>biotinylated</t> goat-anti mouse IgG antibody in combination with streptavidin-HRP (streptavidin horseradish peroxase conjugate) and chemiluminescent substrate. The specific Aβ variants loaded in the different capillaries as single peptides or in mixtures are indicated. Aβ 1–40 , Aβ 2–40 , Aβ 3–40 , Aβ 4–40 , Aβ 5–40 , and Aβ −3–40 were loaded at a concentration of 100 ng/mL. The tested concentration of Aβ N3pE–40 and VKMDAEFRC-bovine serum albumin (BSA) was 200 ng/mL.
    Biotinylated Secondary Antibodies, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 461 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated secondary antibodies/product/Agilent technologies
    Average 92 stars, based on 461 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary antibodies - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier

    90
    Agilent technologies biotinylated secondary antibody swine anti rabbit
    Monoclonal antibody 101-1-1 recognizes Aβ −3–40 with high selectivity on capillary isoelectric focusing immunoassay. A series of synthetic Aβ peptides with different N-termini was separated by isoelectric focusing in microcapillaries, immobilized by a photochemical reaction, and probed with ( a ) mAb 6E10 (2 µg/mL) or ( b ) mAb 101-1-1 (2.1 µg/mL). Chemiluminescent detection was achieved with <t>biotinylated</t> goat-anti mouse IgG antibody in combination with streptavidin-HRP (streptavidin horseradish peroxase conjugate) and chemiluminescent substrate. The specific Aβ variants loaded in the different capillaries as single peptides or in mixtures are indicated. Aβ 1–40 , Aβ 2–40 , Aβ 3–40 , Aβ 4–40 , Aβ 5–40 , and Aβ −3–40 were loaded at a concentration of 100 ng/mL. The tested concentration of Aβ N3pE–40 and VKMDAEFRC-bovine serum albumin (BSA) was 200 ng/mL.
    Biotinylated Secondary Antibody Swine Anti Rabbit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated secondary antibody swine anti rabbit/product/Agilent technologies
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary antibody swine anti rabbit - by Bioz Stars, 2021-01
    90/100 stars
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    Monoclonal antibody 101-1-1 recognizes Aβ −3–40 with high selectivity on capillary isoelectric focusing immunoassay. A series of synthetic Aβ peptides with different N-termini was separated by isoelectric focusing in microcapillaries, immobilized by a photochemical reaction, and probed with ( a ) mAb 6E10 (2 µg/mL) or ( b ) mAb 101-1-1 (2.1 µg/mL). Chemiluminescent detection was achieved with biotinylated goat-anti mouse IgG antibody in combination with streptavidin-HRP (streptavidin horseradish peroxase conjugate) and chemiluminescent substrate. The specific Aβ variants loaded in the different capillaries as single peptides or in mixtures are indicated. Aβ 1–40 , Aβ 2–40 , Aβ 3–40 , Aβ 4–40 , Aβ 5–40 , and Aβ −3–40 were loaded at a concentration of 100 ng/mL. The tested concentration of Aβ N3pE–40 and VKMDAEFRC-bovine serum albumin (BSA) was 200 ng/mL.

    Journal: International Journal of Molecular Sciences

    Article Title: Development and Technical Validation of an Immunoassay for the Detection of APP669–711 (Aβ−3–40) in Biological Samples

    doi: 10.3390/ijms21186564

    Figure Lengend Snippet: Monoclonal antibody 101-1-1 recognizes Aβ −3–40 with high selectivity on capillary isoelectric focusing immunoassay. A series of synthetic Aβ peptides with different N-termini was separated by isoelectric focusing in microcapillaries, immobilized by a photochemical reaction, and probed with ( a ) mAb 6E10 (2 µg/mL) or ( b ) mAb 101-1-1 (2.1 µg/mL). Chemiluminescent detection was achieved with biotinylated goat-anti mouse IgG antibody in combination with streptavidin-HRP (streptavidin horseradish peroxase conjugate) and chemiluminescent substrate. The specific Aβ variants loaded in the different capillaries as single peptides or in mixtures are indicated. Aβ 1–40 , Aβ 2–40 , Aβ 3–40 , Aβ 4–40 , Aβ 5–40 , and Aβ −3–40 were loaded at a concentration of 100 ng/mL. The tested concentration of Aβ N3pE–40 and VKMDAEFRC-bovine serum albumin (BSA) was 200 ng/mL.

    Article Snippet: Biotinylated secondary anti-mouse and anti-rabbit antibodies, used in DAB-immunohistochemical staining, were obtained from DAKO/Agilent (Waldbronn, Germany).

    Techniques: Concentration Assay