biotinylated rabbit anti goat igg  (Vector Laboratories)


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    Name:
    Biotinylated Rabbit Anti Goat IgG Antibody
    Description:
    Biotinylated Rabbit Anti Goat IgG Antibody is prepared using proprietary immunization schedules that produce high affinity antibodies The antibodies are then purified by affinity chromatography and cross reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining ELISAs and blots Biotinylated Rabbit Anti Goat IgG H L is supplied in liquid format With some exceptions the recommended dilution for most applications is 1 200 H L indicates the antibody recognizes both heavy and light chains The use of bovine products such as casein serum albumin or non fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross reactivity of this antibody with bovine immunoglobulins that may be present If this antibody is to be used in these applications blocking with Animal Free Blocker solution Cat No SP 5030 is recommended This antibody is included in the VECTASTAIN ABC kits This antibody is suitable for use with goat sheep and bovine IgG primary antibodies
    Catalog Number:
    ba-5000
    Price:
    None
    Host:
    Rabbit
    Size:
    1 5 mg
    Category:
    Antibodies
    Reactivity:
    Goat
    Buy from Supplier


    Structured Review

    Vector Laboratories biotinylated rabbit anti goat igg
    Biotinylated Rabbit Anti Goat IgG Antibody
    Biotinylated Rabbit Anti Goat IgG Antibody is prepared using proprietary immunization schedules that produce high affinity antibodies The antibodies are then purified by affinity chromatography and cross reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining ELISAs and blots Biotinylated Rabbit Anti Goat IgG H L is supplied in liquid format With some exceptions the recommended dilution for most applications is 1 200 H L indicates the antibody recognizes both heavy and light chains The use of bovine products such as casein serum albumin or non fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross reactivity of this antibody with bovine immunoglobulins that may be present If this antibody is to be used in these applications blocking with Animal Free Blocker solution Cat No SP 5030 is recommended This antibody is included in the VECTASTAIN ABC kits This antibody is suitable for use with goat sheep and bovine IgG primary antibodies
    https://www.bioz.com/result/biotinylated rabbit anti goat igg/product/Vector Laboratories
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    biotinylated rabbit anti goat igg - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Avidin-Biotin Assay:

    Article Title: Fibril specific, conformation dependent antibodies recognize a generic epitope common to amyloid fibrils and fibrillar oligomers that is absent in prefibrillar oligomers
    Article Snippet: .. Following two washes with TBS-A and a wash in TBS-B, sections were incubated in either goat anti-mouse or goat anti-rabbit biotinylated anti-IgG and then in avidin biotin complex (ABC) (Vector Laboratories, Burlingame, CA, USA). .. Antibodies were visualized using 3,3'-diaminobenzidine (DAB, Vector Laboratories).

    Article Title: Ginkgo biloba extract (EGb 761) prevents the ischemic brain injury-induced decrease in parvalbumin expression
    Article Snippet: .. Sections were blocked with 1% normal goat serum in PBS for 1 h and incubated with a rabbit polyclonal anti-rat parvalbumin IgG (diluted 1:200, Cell Signaling Technology, Beverly, MA, USA) at 4℃ for 15 h. After washing with PBS, sections were incubated with biotin-conjugated goat anti-rabbit IgG (1:200 in PBS) for 1 h, followed by incubation with an avidin-biotin-peroxidase complex for 1 h from a Vector ABC Elite kit (Vector Laboratories Inc., Burlingame, CA, USA), incubated with DAB solution with 0.03% hydrogen peroxidase. .. For the positive cell counting, three fields for cortical region were selected from right cerebral cortex.

    Article Title: Growth hormone receptor-deficient pigs resemble the pathophysiology of human Laron syndrome and reveal altered activation of signaling cascades in the liver
    Article Snippet: .. After 3 washing steps in TBS (10 min each), the slides were incubated in biotinylated rabbit anti-goat IgG solution (dilution 1:100, no. BA-5000, Vector Laboratories) for 1 h at room temperature, washed 3 times for 10 min in TBS, and finally incubated with horseradish peroxidase-labelled avidin biotin complex for 30 min (no. PK-6100, VECTASTAIN Elite ABC-Peroxidase kit, Vector Laboratories). ..

    Article Title: Expression of orexin A and its receptor 1 in the human prostate
    Article Snippet: .. Goat polyclonal anti-OXA (sc-8070) and anti-OX1R antibodies (sc-8073), their respective blocking peptides (sc-8070 P, sc-8073 P), and mouse monoclonal anti-chromogranin A (Chr A) antibody (sc-47714) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); rabbit polyclonal anti-prepro-orexin antibody (AB3096), its blocking peptide (AG774) and monoclonal anti-tubulin antibody (MAB1637) from Chemicon International (Temecula, CA, USA); biotinylated rabbit anti-goat secondary antibodies and avidin–biotin complex (PK-6105) from Vector Laboratories (Burlingame, CA, USA); horseradish peroxidase-conjugated anti-goat IgG (A-5420) and anti-rabbit IgG (A-0545) from Sigma Chemical (St Louis, MO, USA). .. Triazol (15596-026) was purchased from Invitrogen (Carlsbad, CA, USA); the enhanced chemiluminescence (ECL) kit (RPN2209), the GFX PCR DNA and Gel Purification Kit (27-9602-01) from Amersham (Little Chalfont, UK); the DC protein assay kit (500-0111) from Bio-Rad Laboratories (Hercules, CA, USA); the primers for human prepro-orexin, OX1R and β-actin from Primm (Milan, Italy); the kit for PCR and RT-PCR (A1280) from Promega (Madison, WI, USA); and ethylenediamine tetra-acetic-acid (EDTA; 60-00-4) from Sigma Aldrich.

    Blocking Assay:

    Article Title: Expression of orexin A and its receptor 1 in the human prostate
    Article Snippet: .. Goat polyclonal anti-OXA (sc-8070) and anti-OX1R antibodies (sc-8073), their respective blocking peptides (sc-8070 P, sc-8073 P), and mouse monoclonal anti-chromogranin A (Chr A) antibody (sc-47714) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); rabbit polyclonal anti-prepro-orexin antibody (AB3096), its blocking peptide (AG774) and monoclonal anti-tubulin antibody (MAB1637) from Chemicon International (Temecula, CA, USA); biotinylated rabbit anti-goat secondary antibodies and avidin–biotin complex (PK-6105) from Vector Laboratories (Burlingame, CA, USA); horseradish peroxidase-conjugated anti-goat IgG (A-5420) and anti-rabbit IgG (A-0545) from Sigma Chemical (St Louis, MO, USA). .. Triazol (15596-026) was purchased from Invitrogen (Carlsbad, CA, USA); the enhanced chemiluminescence (ECL) kit (RPN2209), the GFX PCR DNA and Gel Purification Kit (27-9602-01) from Amersham (Little Chalfont, UK); the DC protein assay kit (500-0111) from Bio-Rad Laboratories (Hercules, CA, USA); the primers for human prepro-orexin, OX1R and β-actin from Primm (Milan, Italy); the kit for PCR and RT-PCR (A1280) from Promega (Madison, WI, USA); and ethylenediamine tetra-acetic-acid (EDTA; 60-00-4) from Sigma Aldrich.

    Incubation:

    Article Title: Fibril specific, conformation dependent antibodies recognize a generic epitope common to amyloid fibrils and fibrillar oligomers that is absent in prefibrillar oligomers
    Article Snippet: .. Following two washes with TBS-A and a wash in TBS-B, sections were incubated in either goat anti-mouse or goat anti-rabbit biotinylated anti-IgG and then in avidin biotin complex (ABC) (Vector Laboratories, Burlingame, CA, USA). .. Antibodies were visualized using 3,3'-diaminobenzidine (DAB, Vector Laboratories).

    Article Title: Ginkgo biloba extract (EGb 761) prevents the ischemic brain injury-induced decrease in parvalbumin expression
    Article Snippet: .. Sections were blocked with 1% normal goat serum in PBS for 1 h and incubated with a rabbit polyclonal anti-rat parvalbumin IgG (diluted 1:200, Cell Signaling Technology, Beverly, MA, USA) at 4℃ for 15 h. After washing with PBS, sections were incubated with biotin-conjugated goat anti-rabbit IgG (1:200 in PBS) for 1 h, followed by incubation with an avidin-biotin-peroxidase complex for 1 h from a Vector ABC Elite kit (Vector Laboratories Inc., Burlingame, CA, USA), incubated with DAB solution with 0.03% hydrogen peroxidase. .. For the positive cell counting, three fields for cortical region were selected from right cerebral cortex.

    Article Title: Growth hormone receptor-deficient pigs resemble the pathophysiology of human Laron syndrome and reveal altered activation of signaling cascades in the liver
    Article Snippet: .. After 3 washing steps in TBS (10 min each), the slides were incubated in biotinylated rabbit anti-goat IgG solution (dilution 1:100, no. BA-5000, Vector Laboratories) for 1 h at room temperature, washed 3 times for 10 min in TBS, and finally incubated with horseradish peroxidase-labelled avidin biotin complex for 30 min (no. PK-6100, VECTASTAIN Elite ABC-Peroxidase kit, Vector Laboratories). ..

    Article Title: Expression and Functional Characterization of GABA Transporters in Crayfish Neurosecretory Cells
    Article Snippet: .. The cells were rinsed three times with PBS and incubated with a goat anti-rabbit biotinylated antibody (Vectastain, Vector Laboratories) at 5 μg/ml in PBS for 40–60 min. .. The cells were incubated with Vectastain ABC reagent (Vector Laboratories) for 30 min.

    Article Title: A novel multiplex assay for simultaneous quantification of total and S129 phosphorylated human alpha-synuclein
    Article Snippet: .. The next day sections were rinsed with KPBS and incubated in 0.25 % Triton + 5 % normal serum in KPBS for 1 h containing secondary antibody biotinylated goat anti rabbit (1:200, Vector Laboratories Inc., USA). .. Sections were again washed with KPBS and incubated with 0.25 % triton + 5 % normal serum in KPBS containing AlexaFluor647 conjugated streptavidin (1:200, Invitrogen, USA) and secondary antibody donkey anti mouse coupled to AlexaFluor488 fluorophore (Jackson, USA) for 2 h. After washes with KPBS, sections were mounted and coverslipped using PVA-DABCO mounting medium (Sigma-Aldrich, Sweden).

    Staining:

    Article Title: Axotomy Upregulates the Anterograde Transport and Expression of Brain-Derived Neurotrophic Factor by Sensory Neurons
    Article Snippet: .. Staining was visualized with biotinylated rabbit anti-goat IgG (diluted 1:500) or biotinylated goat anti-rabbit IgG (diluted 1:200) and the ABC Elite kit for NGF and Standard kit for BONF, CGRP, and SP (Vector, Burlingame, CA), using 3,3′-diaminobenzidine (Sigma, St. Louis, MO) as the chromogen. ..

    Binding Assay:

    Article Title: Imaging of translocator protein upregulation is selective for pro‐inflammatory polarized astrocytes and microglia, et al. Imaging of translocator protein upregulation is selective for pro‐inflammatory polarized astrocytes and microglia
    Article Snippet: .. Primary antibody binding was detected using a goat anti‐rabbit biotinylated antibody (1:100, 30 min, Vector Laboratories) followed by streptavidin‐488 (1:100, 30 min, Invitrogen) and goat anti‐rat texas red (1:100, Vector). .. Cover slips were mounted on Superfrost® Plus slides (Thermoscientific, UK) using DAPI mounting medium for nuclei detection (Vector Laboratories, UK).

    Plasmid Preparation:

    Article Title: Ginkgo biloba extract (EGb 761) prevents the ischemic brain injury-induced decrease in parvalbumin expression
    Article Snippet: .. Sections were blocked with 1% normal goat serum in PBS for 1 h and incubated with a rabbit polyclonal anti-rat parvalbumin IgG (diluted 1:200, Cell Signaling Technology, Beverly, MA, USA) at 4℃ for 15 h. After washing with PBS, sections were incubated with biotin-conjugated goat anti-rabbit IgG (1:200 in PBS) for 1 h, followed by incubation with an avidin-biotin-peroxidase complex for 1 h from a Vector ABC Elite kit (Vector Laboratories Inc., Burlingame, CA, USA), incubated with DAB solution with 0.03% hydrogen peroxidase. .. For the positive cell counting, three fields for cortical region were selected from right cerebral cortex.

    Article Title: Imaging of translocator protein upregulation is selective for pro‐inflammatory polarized astrocytes and microglia, et al. Imaging of translocator protein upregulation is selective for pro‐inflammatory polarized astrocytes and microglia
    Article Snippet: .. Primary antibody binding was detected using a goat anti‐rabbit biotinylated antibody (1:100, 30 min, Vector Laboratories) followed by streptavidin‐488 (1:100, 30 min, Invitrogen) and goat anti‐rat texas red (1:100, Vector). .. Cover slips were mounted on Superfrost® Plus slides (Thermoscientific, UK) using DAPI mounting medium for nuclei detection (Vector Laboratories, UK).

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    Vector Laboratories biotinylated goat anti rabbit immunoglobulin g igg
    Bar graph shows a comparison of the mean area of <t>IgG</t> immunostaining among groups. It must be noted that neither hypothermic intervention nor FK506 administration prevented the spatial extravasation of endogenous IgG. In addition, even the combination
    Biotinylated Goat Anti Rabbit Immunoglobulin G Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 865 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated goat anti rabbit immunoglobulin g igg/product/Vector Laboratories
    Average 99 stars, based on 865 article reviews
    Price from $9.99 to $1999.99
    biotinylated goat anti rabbit immunoglobulin g igg - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    Bar graph shows a comparison of the mean area of IgG immunostaining among groups. It must be noted that neither hypothermic intervention nor FK506 administration prevented the spatial extravasation of endogenous IgG. In addition, even the combination

    Journal: Journal of Cerebral Blood Flow & Metabolism

    Article Title: Combinational therapy using hypothermia and the immunophilin ligand FK506 to target altered pial arteriolar reactivity, axonal damage, and blood-brain barrier dysfunction after traumatic brain injury in rat

    doi: 10.1038/jcbfm.2010.208

    Figure Lengend Snippet: Bar graph shows a comparison of the mean area of IgG immunostaining among groups. It must be noted that neither hypothermic intervention nor FK506 administration prevented the spatial extravasation of endogenous IgG. In addition, even the combination

    Article Snippet: Next, the sections were incubated for 1 hour with biotinylated goat anti-rabbit immunoglobulin G (IgG) (Vector Laboratories Inc., Burlingame, CA, USA) diluted 1:1,000 in 1% normal goat serum in PBS.

    Techniques: Immunostaining

    CD47 Quantification. (A) The modification chemistry summarized in was utilized to facilitate quantification. Following PEI-PDT addition, primary amines were acetylated using sulfo-NHS-Acetate. recCD47 and pepCD47 were biotinylated using Sulfo-NHS-LC-LC-Biotin.

    Journal: Biomaterials

    Article Title: Enhanced biocompatibility of CD47-functionalized vascular stents

    doi: 10.1016/j.biomaterials.2016.02.008

    Figure Lengend Snippet: CD47 Quantification. (A) The modification chemistry summarized in was utilized to facilitate quantification. Following PEI-PDT addition, primary amines were acetylated using sulfo-NHS-Acetate. recCD47 and pepCD47 were biotinylated using Sulfo-NHS-LC-LC-Biotin.

    Article Snippet: The absolute values of CD47 immobilization density were obtained by plotting the OD data generated with the sets of foil samples to a standard OD curve obtained with escalating amounts of biotinylated goat anti-rabbit antibody (Vector Lab), disclosed to contain an average of 10 biotin groups per a 150 kDa molecule.

    Techniques: Modification

    Sodium/iodide symporter (NIS) expression is primarily restricted to striated ducts in human salivary glands. (A) NIS is expressed at a high level along basolateral membranes of striated duct cells (S), with fewer intercalated (rectangle) and excretory (E) duct cells demonstrating lower NIS levels. Acinar (A) cells do not express NIS. Anti-human NIS antibody, avidin-biotin complex, DAB chromogen, hematoxylin counterstain, bar=25 μm. (B) Distribution and intensity of NIS immunoreactivity among intercalated (ID), striated (SD), and excretory (ED) ductal cells in normal human submandibular, parotid, and minor salivary glands. NIS expression is greater and more frequent in striated ducts, regardless of gland type.

    Journal: Thyroid

    Article Title: Modulation of Sodium/Iodide Symporter Expression in the Salivary Gland

    doi: 10.1089/thy.2012.0571

    Figure Lengend Snippet: Sodium/iodide symporter (NIS) expression is primarily restricted to striated ducts in human salivary glands. (A) NIS is expressed at a high level along basolateral membranes of striated duct cells (S), with fewer intercalated (rectangle) and excretory (E) duct cells demonstrating lower NIS levels. Acinar (A) cells do not express NIS. Anti-human NIS antibody, avidin-biotin complex, DAB chromogen, hematoxylin counterstain, bar=25 μm. (B) Distribution and intensity of NIS immunoreactivity among intercalated (ID), striated (SD), and excretory (ED) ductal cells in normal human submandibular, parotid, and minor salivary glands. NIS expression is greater and more frequent in striated ducts, regardless of gland type.

    Article Snippet: The secondary antibody used was a biotinylated goat anti-rabbit antibody (1:200, BA-1000, Vector Laboratories, Inc., Burlingame, CA) that was incubated for 20 minutes at room temperature.

    Techniques: Expressing, Avidin-Biotin Assay

    NIS expression is reduced in inflamed and neoplastic human salivary glands. (A) NIS expression in striated ducts is decreased in inflammatory salivary glands, as well as in benign and malignant neoplasms of ductal origin. (B) NIS immunoreactivity in inflamed (i, ii) and neoplastic (iii, iv) salivary tissue. NIS expression is variably decreased in striated ducts (S) of chronically inflamed salivary glands (i, ii) , particularly in ducts demonstrating goblet metaplasia (arrows). Neoplastic cells of Warthin's tumor (iii) and mucoepidermoid carcinoma (iv) demonstrate lower NIS level, with immunostaining intensity of 2+ and 1+, respectively. Anti-human NIS antibody, avidin-biotin complex, DAB chromogen, hematoxylin counterstain, bars=25 μm. F, periductal fibrosis; L, lymphocytic inflammation.

    Journal: Thyroid

    Article Title: Modulation of Sodium/Iodide Symporter Expression in the Salivary Gland

    doi: 10.1089/thy.2012.0571

    Figure Lengend Snippet: NIS expression is reduced in inflamed and neoplastic human salivary glands. (A) NIS expression in striated ducts is decreased in inflammatory salivary glands, as well as in benign and malignant neoplasms of ductal origin. (B) NIS immunoreactivity in inflamed (i, ii) and neoplastic (iii, iv) salivary tissue. NIS expression is variably decreased in striated ducts (S) of chronically inflamed salivary glands (i, ii) , particularly in ducts demonstrating goblet metaplasia (arrows). Neoplastic cells of Warthin's tumor (iii) and mucoepidermoid carcinoma (iv) demonstrate lower NIS level, with immunostaining intensity of 2+ and 1+, respectively. Anti-human NIS antibody, avidin-biotin complex, DAB chromogen, hematoxylin counterstain, bars=25 μm. F, periductal fibrosis; L, lymphocytic inflammation.

    Article Snippet: The secondary antibody used was a biotinylated goat anti-rabbit antibody (1:200, BA-1000, Vector Laboratories, Inc., Burlingame, CA) that was incubated for 20 minutes at room temperature.

    Techniques: Expressing, Immunostaining, Avidin-Biotin Assay

    Immunoperoxidase staining of B-lymphoma cells after overnight incubation with anti-CD20 (1F5). Cells were deposited on cytocentrifuge slides, fixed with formaldehyde, permeabilised with saponin, and stained with a biotinylated horse anti-mouse IgG, followed by a complex of streptavidin and peroxidase. Observation was with an × 40 objective, except as noted. ( A ) Raji cells, showing prominent staining of JN spots. ( B ) RL cells, showing dark staining of apparently extracellular objects, referred to as CFs. ( C ) A lower-power photograph of RL cells (× 10), to show the general staining pattern. ( D ) RL cells stained in the absence of saponin, to show antigen that is accessible without permeabilisation. Control Abs of the same subclass produced no brown staining.

    Journal: British Journal of Cancer

    Article Title: Antibodies to CD20 and MHC class II antigen bound to B-lymphoma cells accumulate in shed cytoplasmic fragments

    doi: 10.1038/sj.bjc.6602131

    Figure Lengend Snippet: Immunoperoxidase staining of B-lymphoma cells after overnight incubation with anti-CD20 (1F5). Cells were deposited on cytocentrifuge slides, fixed with formaldehyde, permeabilised with saponin, and stained with a biotinylated horse anti-mouse IgG, followed by a complex of streptavidin and peroxidase. Observation was with an × 40 objective, except as noted. ( A ) Raji cells, showing prominent staining of JN spots. ( B ) RL cells, showing dark staining of apparently extracellular objects, referred to as CFs. ( C ) A lower-power photograph of RL cells (× 10), to show the general staining pattern. ( D ) RL cells stained in the absence of saponin, to show antigen that is accessible without permeabilisation. Control Abs of the same subclass produced no brown staining.

    Article Snippet: For use with the rabbit anti-human IgM antisera, we used a biotinylated anti-rabbit IgG from Vector Labs.

    Techniques: Immunoperoxidase Staining, Incubation, Staining, Produced