l pha  (Vector Laboratories)


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    Vector Laboratories l pha
    Results of lectin blotting and western blotting. (A) Lectin blotting validated Sorafenib induced alteration by the <t>biotinylated</t> MAL-I and biotinylated PHA-L. Anti-β-actin antibody was used to show the protein loading. (B) Downregulation of p-ERK in sorafenib-treated MHCC97L and MHCC97H cells. (C) Downregulation of p-ERK in MHCC97L and MHCC97H cells with U0126 treatment. (D) Diagram of the possible mechanism by which sorafenib treatment reduced the expression of Ets-1. sorafenib treatment decreased the expression of Ets-1 by blocking the Ras/Raf/MAPK signaling pathway in HCC cells. MAL-1, Maackia amurensis lecin I; PHA-L, Phaseolus vulgaris <t>leucoagglutinin;</t> p-, phosphorylated; MEK, mitogen activated protein kinase; ERK, extracellular signal-related kinases; Ets-1, erythroblastosis-26; U0126, 1,4-Diamino-2,3-dicyano-1,4-bis (2-aminophenylthio) butadiene.
    L Pha, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l pha/product/Vector Laboratories
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    l pha - by Bioz Stars, 2022-11
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    1) Product Images from "Sorafenib induced alteration of protein glycosylation in hepatocellular carcinoma cells"

    Article Title: Sorafenib induced alteration of protein glycosylation in hepatocellular carcinoma cells

    Journal: Oncology Letters

    doi: 10.3892/ol.2017.6177

    Results of lectin blotting and western blotting. (A) Lectin blotting validated Sorafenib induced alteration by the biotinylated MAL-I and biotinylated PHA-L. Anti-β-actin antibody was used to show the protein loading. (B) Downregulation of p-ERK in sorafenib-treated MHCC97L and MHCC97H cells. (C) Downregulation of p-ERK in MHCC97L and MHCC97H cells with U0126 treatment. (D) Diagram of the possible mechanism by which sorafenib treatment reduced the expression of Ets-1. sorafenib treatment decreased the expression of Ets-1 by blocking the Ras/Raf/MAPK signaling pathway in HCC cells. MAL-1, Maackia amurensis lecin I; PHA-L, Phaseolus vulgaris leucoagglutinin; p-, phosphorylated; MEK, mitogen activated protein kinase; ERK, extracellular signal-related kinases; Ets-1, erythroblastosis-26; U0126, 1,4-Diamino-2,3-dicyano-1,4-bis (2-aminophenylthio) butadiene.
    Figure Legend Snippet: Results of lectin blotting and western blotting. (A) Lectin blotting validated Sorafenib induced alteration by the biotinylated MAL-I and biotinylated PHA-L. Anti-β-actin antibody was used to show the protein loading. (B) Downregulation of p-ERK in sorafenib-treated MHCC97L and MHCC97H cells. (C) Downregulation of p-ERK in MHCC97L and MHCC97H cells with U0126 treatment. (D) Diagram of the possible mechanism by which sorafenib treatment reduced the expression of Ets-1. sorafenib treatment decreased the expression of Ets-1 by blocking the Ras/Raf/MAPK signaling pathway in HCC cells. MAL-1, Maackia amurensis lecin I; PHA-L, Phaseolus vulgaris leucoagglutinin; p-, phosphorylated; MEK, mitogen activated protein kinase; ERK, extracellular signal-related kinases; Ets-1, erythroblastosis-26; U0126, 1,4-Diamino-2,3-dicyano-1,4-bis (2-aminophenylthio) butadiene.

    Techniques Used: Western Blot, Expressing, Blocking Assay

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    • biotinylated lens culinaris agglutinin lca  (Vector Laboratories)
    94
    Vector Laboratories biotinylated lens culinaris agglutinin lca
    Mouse T cells display a sex-dimorphic increase in N-glycan branching with age. a) Fructose 6-phosphate may be metabolized by glycolysis or enter the hexosamine pathway to supply UDP-GlcNAc to the Golgi branching enzymes Mgat1, 2, 4 and 5, which generate mono-, bi-, tri-, and tetra-antennary GlcNAc branched glycans, respectively. The branching enzymes utilize UDP-GlcNAc with declining efficiency such that both Mgat4 and Mgat5 are limited for branching by the metabolic production of UDP-GlcNAc. Small molecule inhibitor kifunensine (KIF) can be used to eliminate N-glycan branching. Plant lectin L-PHA <t>(Phaseolus</t> <t>vulgaris,</t> leukoagglutinin) binding sites are also shown. Abbreviations: OT, oligosaccharyltransferases; GI, glucosidase I; GII, glucosidase II; MI, mannosidase I; MII, mannosidase II; Mgat, N-acetylglucosaminyltransferase; GalT3, galactosyltransferase 3; iGnT, i-branching enzyme β1,3-N-acetylglucosaminyltransferase; KIF, kifunensine; GlcNAc, N-acetylglucosamine; UDP, uridine diphosphate; Km, Michaelis constant of the enzyme. b) The gating strategy is demonstrated for CD4 + T N cells. Lymphocytes were first gated on singlets, followed by gating on CD3 + CD4 + CD8 − CD25 − CD62L + CD44 − cells by sequential steps. c, d) Splenocytes from five young and old mice were analyzed for L-PHA ( c ) or ConA ( d ) binding by flow cytometry, gating on the indicated CD4 + T cell subsets. Absolute geometric mean fluorescence intensity (MFI) is shown to allow direct comparison between naïve and memory subsets. e-i) CD4 + T N cells ( e-g ) CD19 + B cells ( h ) or thymocytes ( i ) were obtained from the lymph node ( e ), spleen ( f-h ) or thymus ( i ) of female ( e, g-i ) or male ( f-h ) mice of the indicated ages, and analyzed for L-PHA binding by flow cytometry. Absolute or normalized geometric mean fluorescence intensity (MFI) are shown. Each symbol represents a single mouse. P-values by two-tailed Mann-Whitney ( c, d ) or two-tailed Wilcoxon ( e, h, i ). Error bars indicate mean ± s.e.m.
    Biotinylated Lens Culinaris Agglutinin Lca, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated lens culinaris agglutinin lca/product/Vector Laboratories
    Average 94 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    biotinylated lens culinaris agglutinin lca - by Bioz Stars, 2022-11
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    Mouse T cells display a sex-dimorphic increase in N-glycan branching with age. a) Fructose 6-phosphate may be metabolized by glycolysis or enter the hexosamine pathway to supply UDP-GlcNAc to the Golgi branching enzymes Mgat1, 2, 4 and 5, which generate mono-, bi-, tri-, and tetra-antennary GlcNAc branched glycans, respectively. The branching enzymes utilize UDP-GlcNAc with declining efficiency such that both Mgat4 and Mgat5 are limited for branching by the metabolic production of UDP-GlcNAc. Small molecule inhibitor kifunensine (KIF) can be used to eliminate N-glycan branching. Plant lectin L-PHA (Phaseolus vulgaris, leukoagglutinin) binding sites are also shown. Abbreviations: OT, oligosaccharyltransferases; GI, glucosidase I; GII, glucosidase II; MI, mannosidase I; MII, mannosidase II; Mgat, N-acetylglucosaminyltransferase; GalT3, galactosyltransferase 3; iGnT, i-branching enzyme β1,3-N-acetylglucosaminyltransferase; KIF, kifunensine; GlcNAc, N-acetylglucosamine; UDP, uridine diphosphate; Km, Michaelis constant of the enzyme. b) The gating strategy is demonstrated for CD4 + T N cells. Lymphocytes were first gated on singlets, followed by gating on CD3 + CD4 + CD8 − CD25 − CD62L + CD44 − cells by sequential steps. c, d) Splenocytes from five young and old mice were analyzed for L-PHA ( c ) or ConA ( d ) binding by flow cytometry, gating on the indicated CD4 + T cell subsets. Absolute geometric mean fluorescence intensity (MFI) is shown to allow direct comparison between naïve and memory subsets. e-i) CD4 + T N cells ( e-g ) CD19 + B cells ( h ) or thymocytes ( i ) were obtained from the lymph node ( e ), spleen ( f-h ) or thymus ( i ) of female ( e, g-i ) or male ( f-h ) mice of the indicated ages, and analyzed for L-PHA binding by flow cytometry. Absolute or normalized geometric mean fluorescence intensity (MFI) are shown. Each symbol represents a single mouse. P-values by two-tailed Mann-Whitney ( c, d ) or two-tailed Wilcoxon ( e, h, i ). Error bars indicate mean ± s.e.m.

    Journal: Nature aging

    Article Title: Age-associated impairment of T cell immunity is linked to sex-dimorphic elevation of N-glycan branching

    doi: 10.1038/s43587-022-00187-y

    Figure Lengend Snippet: Mouse T cells display a sex-dimorphic increase in N-glycan branching with age. a) Fructose 6-phosphate may be metabolized by glycolysis or enter the hexosamine pathway to supply UDP-GlcNAc to the Golgi branching enzymes Mgat1, 2, 4 and 5, which generate mono-, bi-, tri-, and tetra-antennary GlcNAc branched glycans, respectively. The branching enzymes utilize UDP-GlcNAc with declining efficiency such that both Mgat4 and Mgat5 are limited for branching by the metabolic production of UDP-GlcNAc. Small molecule inhibitor kifunensine (KIF) can be used to eliminate N-glycan branching. Plant lectin L-PHA (Phaseolus vulgaris, leukoagglutinin) binding sites are also shown. Abbreviations: OT, oligosaccharyltransferases; GI, glucosidase I; GII, glucosidase II; MI, mannosidase I; MII, mannosidase II; Mgat, N-acetylglucosaminyltransferase; GalT3, galactosyltransferase 3; iGnT, i-branching enzyme β1,3-N-acetylglucosaminyltransferase; KIF, kifunensine; GlcNAc, N-acetylglucosamine; UDP, uridine diphosphate; Km, Michaelis constant of the enzyme. b) The gating strategy is demonstrated for CD4 + T N cells. Lymphocytes were first gated on singlets, followed by gating on CD3 + CD4 + CD8 − CD25 − CD62L + CD44 − cells by sequential steps. c, d) Splenocytes from five young and old mice were analyzed for L-PHA ( c ) or ConA ( d ) binding by flow cytometry, gating on the indicated CD4 + T cell subsets. Absolute geometric mean fluorescence intensity (MFI) is shown to allow direct comparison between naïve and memory subsets. e-i) CD4 + T N cells ( e-g ) CD19 + B cells ( h ) or thymocytes ( i ) were obtained from the lymph node ( e ), spleen ( f-h ) or thymus ( i ) of female ( e, g-i ) or male ( f-h ) mice of the indicated ages, and analyzed for L-PHA binding by flow cytometry. Absolute or normalized geometric mean fluorescence intensity (MFI) are shown. Each symbol represents a single mouse. P-values by two-tailed Mann-Whitney ( c, d ) or two-tailed Wilcoxon ( e, h, i ). Error bars indicate mean ± s.e.m.

    Article Snippet: For ex vivo and in vitro mouse experiments, erythrocytes were depleted from splenic or lymph node cell suspensions by incubation with red blood cell (RBC) lysis buffer followed by negative selection using EasySep™ Naïve CD4+ T Cell Isolation Kit or EasySep™ CD4+ T Cell Isolation Kit (STEMCELL Technologies) according to manufacturer’s instructions and supplemented with or without 20μg/mL biotinylated Phaseolus Vulgaris Leucoagglutinin (L-PHA, Vector Labs) to deplete non- Mgat2 deleted cells.

    Techniques: Binding Assay, Mouse Assay, Flow Cytometry, Fluorescence, Two Tailed Test, MANN-WHITNEY