biotinylated mouse secondary antibody  (Vector Laboratories)


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    Name:
    Mouse on Mouse M O M Biotinylated Anti Mouse Ig Reagent
    Description:
    M O M Mouse on Mouse Anti Mouse Reagent is a specially modified secondary antibody that has been optimized specifically for use with the Vector M O M Immunodetection Kit components This is the same reagent contained in the Vector M O M Kits Cat Nos PK 2200 FMK 2201 and BMK 2202 The Vector M O M Immunodetection Kits are specifically designed to reduce endogenous mouse IgG staining when using mouse primary antibodies on mouse tissue The inability of an anti mouse secondary antibody to distinguish between a primary antibody produced in mice and the endogenous mouse immunoglobulins present in mouse tissue results in high background staining which obscures specific staining This problem can be essentially eliminated by using a Vector M O M Kit The result is clear crisp specific staining of the antigens of interest Excellent staining results for a once difficult application have now become routine with the Vector M O M System
    Catalog Number:
    mkb-2225
    Price:
    None
    Category:
    Antibodies
    Reactivity:
    Mouse
    Size:
    0 1 ml
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    Structured Review

    Vector Laboratories biotinylated mouse secondary antibody
    Mouse on Mouse M O M Biotinylated Anti Mouse Ig Reagent
    M O M Mouse on Mouse Anti Mouse Reagent is a specially modified secondary antibody that has been optimized specifically for use with the Vector M O M Immunodetection Kit components This is the same reagent contained in the Vector M O M Kits Cat Nos PK 2200 FMK 2201 and BMK 2202 The Vector M O M Immunodetection Kits are specifically designed to reduce endogenous mouse IgG staining when using mouse primary antibodies on mouse tissue The inability of an anti mouse secondary antibody to distinguish between a primary antibody produced in mice and the endogenous mouse immunoglobulins present in mouse tissue results in high background staining which obscures specific staining This problem can be essentially eliminated by using a Vector M O M Kit The result is clear crisp specific staining of the antigens of interest Excellent staining results for a once difficult application have now become routine with the Vector M O M System
    https://www.bioz.com/result/biotinylated mouse secondary antibody/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated mouse secondary antibody - by Bioz Stars, 2021-03
    99/100 stars

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    Incubation:

    Article Title: Co-treatment with therapeutic neural stem cells expressing carboxyl esterase and CPT-11 inhibit growth of primary and metastatic lung cancers in mice
    Article Snippet: Samples were subsequently incubated with a primary mouse monoclonal antibody against proliferating cell nuclear antigen (PCNA, 1:100, Abcam, plc., Cambridge, UK) for overnight at 4°C. .. After four washes for 10 min in PBS-Tween, the slides were incubated in the biotinylated anti-mouse secondary antibody (1:500 dilution, Vector Laboratories) solution for 30 min at 37°C. .. To form the immunoreactive complex, ABC kit reagent (Vectastain Universal Elite ABC kit, Vector Laboratories) was treated at each slide for 30 min. After DAB substrate (Sigma-Aldrich Co.) and hematoxylin staining solution treatment, all slides were detected under a BX51 light microscope for digital photography.

    Article Title: Inflammatory properties of inhibitor of DNA binding 1 secreted by synovial fibroblasts in rheumatoid arthritis
    Article Snippet: The tissue sections were blocked with 5 % donkey serum and 20 % fetal bovine serum (FBS) in phosphate-buffered saline (PBS) at 37 °C for 1 h. The sections were then incubated with either mouse anti-human Id1 antibody (Abcam, Cambridge, MA, USA, 10 μg/mL), rabbit anti-mouse Id1 antibody (CalBioreagents, San Mateo, CA, USA, 10 μg/mL), or purified nonspecific mouse and rabbit immunoglobulin G (IgG) (Thermo Fisher Scientific, Waltham, MA, USA) for 1 h at 37 °C in blocking buffer. .. After washing, tissues were incubated with a biotinylated anti-mouse or anti-rabbit secondary antibody (Vector Laboratories, Burlingame, CA, USA, 10 μg/mL) for 1 h at 37 °C in blocking buffer. .. Vectastain ABC kit (Vector Laboratories) was used to detect the antibodies on the tissues, following manufacturer’s protocols.

    Article Title: Human Osteogenesis Involves Differentiation-Dependent Increases in the Morphogenically Active 3? Alternative Splicing Variant of Acetylcholinesterase
    Article Snippet: Findings were expressed as mean ± standard error, and analysis of variance (ANOVA) was performed with the superANOVA statistical package (Abacus Concepts, Inc., Berkeley, Calif.). .. For immunohistochemistry, cryocut sections were incubated with human-specific anti-AChE monoclonal antibody 101-1 (primary antibody) ( ) and biotinylated anti-mouse antibody (secondary antibody) (Vectastain; Vector Laboratories, Burlingame, Calif.); detection was carried out with 3,3′-diaminobenzidine and urea-H2 O2 as instructed by the manufacturer (Sigma). .. Cytochemical staining for AChE activity on nonfixed cells grown on glass slides and on cryocut sections, as well as determination of hydrolysis rates of acetylthiocholine iodide in homogenates, was performed essentially as detailed elsewhere ( ) for the noted time periods.

    Article Title: Host-Specific Glycans Are Correlated with Susceptibility to Infection by Lagoviruses, but Not with Their Virulence
    Article Snippet: B/EBHS/6-infected liver homogenate diluted 1/5 in PBS-1% BSA was then added and incubated at 4°C overnight. .. After 3 washes with PBS, monoclonal anti-EBHSV antibody (5F5; a kind gift from L. Capucci, IZSLER, Brescia, Italy) was added at 1/100 dilution for 2 h at 37°C, followed by 3 washes with PBS and incubation with biotinylated anti-mouse antibody (Vector Laboratories) at 1/1,000 dilution for 2 h at 37°C. .. The sequence for the capsid protein gene of isolate B-EBHS-6 is available in GenBank under accession number .

    Article Title: Anatomical Plasticity of the Distal Forelimb Projection of the Ventral Premotor Cortex Four weeks After Primary Motor Cortex Injury
    Article Snippet: The first series was incubated in 5% normal goat serum rinse buffer overnight then incubated in ABC solution for 4 hours then reacted with 3-3” diaminobenzidine (DAB) to visualize BDA ( ; ) ( , ), the second series was incubated with a monoclonal primary antibody raised in mouse directed against the neuronal marker NeuN (Chemicon, Temecula, CA) for 48 hours at a 1:1000 ratio. .. Following the 48 hour primary antibody incubation, tissue sections were rinsed and incubated at room temperature in a secondary biotinylated anti-mouse antibody for 4 hours (Vector Laboratories, Burlingame, CA). .. This series was then incubated with ABC solution and reacted with Vector SG (Vector Laboratories, Burlingame,CA) ( ).

    Infection:

    Article Title: Antibodies to several conformation-dependent epitopes of gp120/gp41 inhibit CCR-5-dependent cell-to-cell fusion mediated by the native envelope glycoprotein of a primary macrophage-tropic HIV-1 isolate
    Article Snippet: .. Virus stocks were titered on BHK cells, infection was detected by immunohistostaining with mAbs 41A and F5.5 (1 μg/ml) as primary antibodies, followed by biotinylated anti-mouse IgG then avidin and biotinylated horseradish peroxidase H (Vectastain ABC kit; Vector Laboratories). .. P4 (CCR-5− ) or P4P (CCR-5+ ) HeLa cells, at a density of 2 × 105 cells per well in a 24-multiwell plate, were washed with DMEM without serum and infected with defective recombinant SFV–LAI, SFV–BX08, or SFV–LacZ at a multiplicity of infection of 3.6 infectious particles per cell (as measured on BHK cells).

    Avidin-Biotin Assay:

    Article Title: Antibodies to several conformation-dependent epitopes of gp120/gp41 inhibit CCR-5-dependent cell-to-cell fusion mediated by the native envelope glycoprotein of a primary macrophage-tropic HIV-1 isolate
    Article Snippet: .. Virus stocks were titered on BHK cells, infection was detected by immunohistostaining with mAbs 41A and F5.5 (1 μg/ml) as primary antibodies, followed by biotinylated anti-mouse IgG then avidin and biotinylated horseradish peroxidase H (Vectastain ABC kit; Vector Laboratories). .. P4 (CCR-5− ) or P4P (CCR-5+ ) HeLa cells, at a density of 2 × 105 cells per well in a 24-multiwell plate, were washed with DMEM without serum and infected with defective recombinant SFV–LAI, SFV–BX08, or SFV–LacZ at a multiplicity of infection of 3.6 infectious particles per cell (as measured on BHK cells).

    Blocking Assay:

    Article Title: Inflammatory properties of inhibitor of DNA binding 1 secreted by synovial fibroblasts in rheumatoid arthritis
    Article Snippet: The tissue sections were blocked with 5 % donkey serum and 20 % fetal bovine serum (FBS) in phosphate-buffered saline (PBS) at 37 °C for 1 h. The sections were then incubated with either mouse anti-human Id1 antibody (Abcam, Cambridge, MA, USA, 10 μg/mL), rabbit anti-mouse Id1 antibody (CalBioreagents, San Mateo, CA, USA, 10 μg/mL), or purified nonspecific mouse and rabbit immunoglobulin G (IgG) (Thermo Fisher Scientific, Waltham, MA, USA) for 1 h at 37 °C in blocking buffer. .. After washing, tissues were incubated with a biotinylated anti-mouse or anti-rabbit secondary antibody (Vector Laboratories, Burlingame, CA, USA, 10 μg/mL) for 1 h at 37 °C in blocking buffer. .. Vectastain ABC kit (Vector Laboratories) was used to detect the antibodies on the tissues, following manufacturer’s protocols.

    Immunohistochemistry:

    Article Title: Human Osteogenesis Involves Differentiation-Dependent Increases in the Morphogenically Active 3? Alternative Splicing Variant of Acetylcholinesterase
    Article Snippet: Findings were expressed as mean ± standard error, and analysis of variance (ANOVA) was performed with the superANOVA statistical package (Abacus Concepts, Inc., Berkeley, Calif.). .. For immunohistochemistry, cryocut sections were incubated with human-specific anti-AChE monoclonal antibody 101-1 (primary antibody) ( ) and biotinylated anti-mouse antibody (secondary antibody) (Vectastain; Vector Laboratories, Burlingame, Calif.); detection was carried out with 3,3′-diaminobenzidine and urea-H2 O2 as instructed by the manufacturer (Sigma). .. Cytochemical staining for AChE activity on nonfixed cells grown on glass slides and on cryocut sections, as well as determination of hydrolysis rates of acetylthiocholine iodide in homogenates, was performed essentially as detailed elsewhere ( ) for the noted time periods.

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    Vector Laboratories biotinylated secondary antibodies
    D 2 and D 3 immunocytochemistry were performed on the submucosal plexus of the ileum of CD-1 mice. The same tissue preparation from D 2 and D 3 KO mice was used as control. D 2 receptor immunoreactivity (IR) was revealed by a D 2 rabbit antibody and a donkey anti-rabbit Alexa 594 secondary antibody. D 3 receptor immunoreactivity was revealed by a D 3 goat antibody, a <t>biotinylated</t> donkey anti-goat secondary antibody, and streptavidin FITC. On the tissue of CD-1 mice, D 2 -immunoreactive products were present in the enteric neurons ( A ), and D 3 -immunoreactive products were also present in the enteric neurons ( B ). D 2 and D 3 immunoreactivities were colocalized in the same cell ( C ). However, D 2 immunoreactivity was not detected on the tissue of D 2 knock-out (KO) mice ( D ); no D 3 immunoreactivity was detected on the tissue of D 3 knock-out mouse ( E ). The arrows indicate the immunoreactive neurons. Scale bar: (in C ) 25 μm.
    Biotinylated Secondary Antibodies, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated secondary antibodies/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary antibodies - by Bioz Stars, 2021-03
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    99
    Vector Laboratories biotinylated horse anti mouse ig secondary ab
    Differences in serum gp70 expression and glomerular pathology induced after injection of purified 12H5.1 IgG3 in NZW, (BALB/c × MRL/+)F 1 , and B6 mice. (a) Results of Western blotting assays showing the expression of serum gp70 in three different strains of mice. Sera were diluted 1:20 into SDS sample buffer without a reducing reagent and boiled for 5 min; 10 μl of each boiled mixture was loaded into a well of 7.5% polyacrylamide gel. Plasma from a 4 month-old female MRL/ lpr ), NZW mice expressed a high level of serum gp85 (gp70 plus p15E), gp70, and a degradation product gp45 (arrowheads), while their expression in B6 mice was low. (BALB/c × MRL/+)F 1 mice (F 1 ) expressed an intermediate level of serum gp70. Mr, <t>biotinylated</t> markers, with positions indicated in kilodaltons at the left. (b to d) Representative photomicrographs taken from kidney sections of NZW (b), (BALB/c × MRL/+)F 1 (c), and B6 (d) mice injected with purified anti-gp70 IgG3, 12H5.1; hematoxylin and eosin staining, ×300. Note apparent thickening of the capillary walls (arrowheads) and inflammatory cell infiltration (arrow) in panel b and marked increase in glomerular cellularity and evident neutrophilic infiltration in panel c.
    Biotinylated Horse Anti Mouse Ig Secondary Ab, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated horse anti mouse ig secondary ab/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated horse anti mouse ig secondary ab - by Bioz Stars, 2021-03
    99/100 stars
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    D 2 and D 3 immunocytochemistry were performed on the submucosal plexus of the ileum of CD-1 mice. The same tissue preparation from D 2 and D 3 KO mice was used as control. D 2 receptor immunoreactivity (IR) was revealed by a D 2 rabbit antibody and a donkey anti-rabbit Alexa 594 secondary antibody. D 3 receptor immunoreactivity was revealed by a D 3 goat antibody, a biotinylated donkey anti-goat secondary antibody, and streptavidin FITC. On the tissue of CD-1 mice, D 2 -immunoreactive products were present in the enteric neurons ( A ), and D 3 -immunoreactive products were also present in the enteric neurons ( B ). D 2 and D 3 immunoreactivities were colocalized in the same cell ( C ). However, D 2 immunoreactivity was not detected on the tissue of D 2 knock-out (KO) mice ( D ); no D 3 immunoreactivity was detected on the tissue of D 3 knock-out mouse ( E ). The arrows indicate the immunoreactive neurons. Scale bar: (in C ) 25 μm.

    Journal: The Journal of Neuroscience

    Article Title: Physiological Modulation of Intestinal Motility by Enteric Dopaminergic Neurons and the D2 Receptor: Analysis of Dopamine Receptor Expression, Location, Development, and Function in Wild-Type and Knock-Out Mice

    doi: 10.1523/JNEUROSCI.4720-05.2006

    Figure Lengend Snippet: D 2 and D 3 immunocytochemistry were performed on the submucosal plexus of the ileum of CD-1 mice. The same tissue preparation from D 2 and D 3 KO mice was used as control. D 2 receptor immunoreactivity (IR) was revealed by a D 2 rabbit antibody and a donkey anti-rabbit Alexa 594 secondary antibody. D 3 receptor immunoreactivity was revealed by a D 3 goat antibody, a biotinylated donkey anti-goat secondary antibody, and streptavidin FITC. On the tissue of CD-1 mice, D 2 -immunoreactive products were present in the enteric neurons ( A ), and D 3 -immunoreactive products were also present in the enteric neurons ( B ). D 2 and D 3 immunoreactivities were colocalized in the same cell ( C ). However, D 2 immunoreactivity was not detected on the tissue of D 2 knock-out (KO) mice ( D ); no D 3 immunoreactivity was detected on the tissue of D 3 knock-out mouse ( E ). The arrows indicate the immunoreactive neurons. Scale bar: (in C ) 25 μm.

    Article Snippet: The blocked tissue was then incubated overnight with primary antibodies, washed with PBS, and exposed with intervening washes in PBS to the biotinylated secondary antibodies (2 h), 0.3% H2 O2 in 0.3% blocking sera (5 min), Elite ABC reagent (1 h; Vector Laboratories), and 3′,5′-diaminobenzidine solution (2–10 min) until suitable staining developed.

    Techniques: Immunocytochemistry, Mouse Assay, Knock-Out

    Avastin attenuates astrogliosis and blood vessel formation in the injured cortex. a, c Cortical sections were obtained from mice in which Avastin or PBS was infused into the ventricle 14 d post-ATP injection. GFAP and CoL1A1 were visualized with Alexa-488 and Alexa-594 conjugated secondary antibodies ( a ) or biotinylated secondary antibodies and DAB-based color reaction ( c ). b Relative fluorescence intensity of GFAP and CoL1A1 were measured using ZEN software and plotted. Values are means ± SEMs for animals treated with saline ( n = 4) or Avastin ( n = 3) (*, p

    Journal: Molecular Brain

    Article Title: Region-specific astrogliosis: differential vessel formation contributes to different patterns of astrogliosis in the cortex and striatum

    doi: 10.1186/s13041-020-00642-0

    Figure Lengend Snippet: Avastin attenuates astrogliosis and blood vessel formation in the injured cortex. a, c Cortical sections were obtained from mice in which Avastin or PBS was infused into the ventricle 14 d post-ATP injection. GFAP and CoL1A1 were visualized with Alexa-488 and Alexa-594 conjugated secondary antibodies ( a ) or biotinylated secondary antibodies and DAB-based color reaction ( c ). b Relative fluorescence intensity of GFAP and CoL1A1 were measured using ZEN software and plotted. Values are means ± SEMs for animals treated with saline ( n = 4) or Avastin ( n = 3) (*, p

    Article Snippet: The sections were rinsed three times with PBS and incubated with biotinylated secondary antibodies (Vector Laboratories, Burlingame, CA, USA).

    Techniques: Mouse Assay, Injection, Fluorescence, Software

    Expression of heme oxygenase-1 (HO-1) in WT and Sftpd −/− mice. Lung sections, prepared 72 h after exposure of 8-, 27-, and 80-wk-old WT and Sftpd −/− mice to air or ozone, were stained with antibody to HO-1 or IgG control followed by biotinylated secondary antibody. Binding was visualized by use of a peroxidase substrate DAB kit. One representative section from 3 separate experiments is shown ( n = 3 mice/treatment group). Original magnification, ×600.

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Age-related increases in ozone-induced injury and altered pulmonary mechanics in mice with progressive lung inflammation

    doi: 10.1152/ajplung.00027.2013

    Figure Lengend Snippet: Expression of heme oxygenase-1 (HO-1) in WT and Sftpd −/− mice. Lung sections, prepared 72 h after exposure of 8-, 27-, and 80-wk-old WT and Sftpd −/− mice to air or ozone, were stained with antibody to HO-1 or IgG control followed by biotinylated secondary antibody. Binding was visualized by use of a peroxidase substrate DAB kit. One representative section from 3 separate experiments is shown ( n = 3 mice/treatment group). Original magnification, ×600.

    Article Snippet: Sections were then incubated with biotinylated secondary antibody (1:200, Vector Labs, Burlingame, CA) for 30 min. A DAB Peroxidase Substrate Kit (Vector Labs) was used to visualize binding.

    Techniques: Expressing, Mouse Assay, Staining, Binding Assay

    Expression of mannose receptor in WT and Sftpd −/− mice. Lung sections, prepared 72 h after exposure of 8-, 27-, and 80-wk-old WT and Sftpd −/− mice to air or ozone, were stained with antibody to mannose receptor or IgG control followed by biotinylated secondary antibody. Binding was visualized by use of a peroxidase substrate DAB kit. One representative section from 3 separate experiments is shown ( n = 3 mice/treatment group). Original magnification, ×600.

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Age-related increases in ozone-induced injury and altered pulmonary mechanics in mice with progressive lung inflammation

    doi: 10.1152/ajplung.00027.2013

    Figure Lengend Snippet: Expression of mannose receptor in WT and Sftpd −/− mice. Lung sections, prepared 72 h after exposure of 8-, 27-, and 80-wk-old WT and Sftpd −/− mice to air or ozone, were stained with antibody to mannose receptor or IgG control followed by biotinylated secondary antibody. Binding was visualized by use of a peroxidase substrate DAB kit. One representative section from 3 separate experiments is shown ( n = 3 mice/treatment group). Original magnification, ×600.

    Article Snippet: Sections were then incubated with biotinylated secondary antibody (1:200, Vector Labs, Burlingame, CA) for 30 min. A DAB Peroxidase Substrate Kit (Vector Labs) was used to visualize binding.

    Techniques: Expressing, Mouse Assay, Staining, Binding Assay

    Expression of YM-1 in WT and Sftpd −/− mice. Lung sections, prepared 72 h after exposure of 8-, 27-, and 80-wk-old WT and Sftpd −/− mice to air or ozone, were stained with antibody to YM-1 or IgG control followed by biotinylated secondary antibody. Binding was visualized by use of a peroxidase substrate DAB kit. One representative section from 3 separate experiments is shown ( n = 3 mice/treatment group). Original magnification, ×600.

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Age-related increases in ozone-induced injury and altered pulmonary mechanics in mice with progressive lung inflammation

    doi: 10.1152/ajplung.00027.2013

    Figure Lengend Snippet: Expression of YM-1 in WT and Sftpd −/− mice. Lung sections, prepared 72 h after exposure of 8-, 27-, and 80-wk-old WT and Sftpd −/− mice to air or ozone, were stained with antibody to YM-1 or IgG control followed by biotinylated secondary antibody. Binding was visualized by use of a peroxidase substrate DAB kit. One representative section from 3 separate experiments is shown ( n = 3 mice/treatment group). Original magnification, ×600.

    Article Snippet: Sections were then incubated with biotinylated secondary antibody (1:200, Vector Labs, Burlingame, CA) for 30 min. A DAB Peroxidase Substrate Kit (Vector Labs) was used to visualize binding.

    Techniques: Expressing, Mouse Assay, Staining, Binding Assay

    Expression of galectin-3 in WT and Sftpd −/− mice. Lung sections, prepared 72 h after exposure of 8-, 27-, and 80-wk-old WT and Sftpd −/− mice to air or ozone, were stained with antibody to galectin-3 or IgG control followed by biotinylated secondary antibody. Binding was visualized by use of a peroxidase substrate DAB kit. One representative section from 3 separate experiments is shown ( n = 3 mice/treatment group). Original magnification, ×600.

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Age-related increases in ozone-induced injury and altered pulmonary mechanics in mice with progressive lung inflammation

    doi: 10.1152/ajplung.00027.2013

    Figure Lengend Snippet: Expression of galectin-3 in WT and Sftpd −/− mice. Lung sections, prepared 72 h after exposure of 8-, 27-, and 80-wk-old WT and Sftpd −/− mice to air or ozone, were stained with antibody to galectin-3 or IgG control followed by biotinylated secondary antibody. Binding was visualized by use of a peroxidase substrate DAB kit. One representative section from 3 separate experiments is shown ( n = 3 mice/treatment group). Original magnification, ×600.

    Article Snippet: Sections were then incubated with biotinylated secondary antibody (1:200, Vector Labs, Burlingame, CA) for 30 min. A DAB Peroxidase Substrate Kit (Vector Labs) was used to visualize binding.

    Techniques: Expressing, Mouse Assay, Staining, Binding Assay

    Expression of pro-surfactant protein C (SP-C) in WT and Sftpd −/− mice. Lung sections, prepared 72 h after exposure of 8-, 27-, and 80-wk-old WT and Sftpd −/− mice to air or ozone, were stained with antibody to pro-SP-C or IgG control followed by biotinylated secondary antibody. Binding was visualized by use of a peroxidase substrate DAB kit. One representative section from 3 separate experiments is shown ( n = 3 mice/treatment group). Arrowheads indicate insets. Original magnification, ×600; inset, ×1,000.

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Age-related increases in ozone-induced injury and altered pulmonary mechanics in mice with progressive lung inflammation

    doi: 10.1152/ajplung.00027.2013

    Figure Lengend Snippet: Expression of pro-surfactant protein C (SP-C) in WT and Sftpd −/− mice. Lung sections, prepared 72 h after exposure of 8-, 27-, and 80-wk-old WT and Sftpd −/− mice to air or ozone, were stained with antibody to pro-SP-C or IgG control followed by biotinylated secondary antibody. Binding was visualized by use of a peroxidase substrate DAB kit. One representative section from 3 separate experiments is shown ( n = 3 mice/treatment group). Arrowheads indicate insets. Original magnification, ×600; inset, ×1,000.

    Article Snippet: Sections were then incubated with biotinylated secondary antibody (1:200, Vector Labs, Burlingame, CA) for 30 min. A DAB Peroxidase Substrate Kit (Vector Labs) was used to visualize binding.

    Techniques: Expressing, Mouse Assay, Staining, Binding Assay

    Expression of inducible nitric oxide synthase (iNOS) in WT and Sftpd −/− mice. Lung sections, prepared 72 h after exposure of 8-, 27-, and 80-wk-old WT and Sftpd −/− mice to air or ozone, were stained with antibody to iNOS or IgG control followed by biotinylated secondary antibody. Binding was visualized by use of a peroxidase substrate DAB kit. One representative section from 3 separate experiments is shown ( n = 3 mice/treatment group). Original magnification, ×600.

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Age-related increases in ozone-induced injury and altered pulmonary mechanics in mice with progressive lung inflammation

    doi: 10.1152/ajplung.00027.2013

    Figure Lengend Snippet: Expression of inducible nitric oxide synthase (iNOS) in WT and Sftpd −/− mice. Lung sections, prepared 72 h after exposure of 8-, 27-, and 80-wk-old WT and Sftpd −/− mice to air or ozone, were stained with antibody to iNOS or IgG control followed by biotinylated secondary antibody. Binding was visualized by use of a peroxidase substrate DAB kit. One representative section from 3 separate experiments is shown ( n = 3 mice/treatment group). Original magnification, ×600.

    Article Snippet: Sections were then incubated with biotinylated secondary antibody (1:200, Vector Labs, Burlingame, CA) for 30 min. A DAB Peroxidase Substrate Kit (Vector Labs) was used to visualize binding.

    Techniques: Expressing, Mouse Assay, Staining, Binding Assay

    Differences in serum gp70 expression and glomerular pathology induced after injection of purified 12H5.1 IgG3 in NZW, (BALB/c × MRL/+)F 1 , and B6 mice. (a) Results of Western blotting assays showing the expression of serum gp70 in three different strains of mice. Sera were diluted 1:20 into SDS sample buffer without a reducing reagent and boiled for 5 min; 10 μl of each boiled mixture was loaded into a well of 7.5% polyacrylamide gel. Plasma from a 4 month-old female MRL/ lpr ), NZW mice expressed a high level of serum gp85 (gp70 plus p15E), gp70, and a degradation product gp45 (arrowheads), while their expression in B6 mice was low. (BALB/c × MRL/+)F 1 mice (F 1 ) expressed an intermediate level of serum gp70. Mr, biotinylated markers, with positions indicated in kilodaltons at the left. (b to d) Representative photomicrographs taken from kidney sections of NZW (b), (BALB/c × MRL/+)F 1 (c), and B6 (d) mice injected with purified anti-gp70 IgG3, 12H5.1; hematoxylin and eosin staining, ×300. Note apparent thickening of the capillary walls (arrowheads) and inflammatory cell infiltration (arrow) in panel b and marked increase in glomerular cellularity and evident neutrophilic infiltration in panel c.

    Journal: Journal of Virology

    Article Title: Establishment of Monoclonal Anti-Retroviral gp70 Autoantibodies from MRL/lpr Lupus Mice and Induction of Glomerular gp70 Deposition and Pathology by Transfer into Non-Autoimmune Mice

    doi:

    Figure Lengend Snippet: Differences in serum gp70 expression and glomerular pathology induced after injection of purified 12H5.1 IgG3 in NZW, (BALB/c × MRL/+)F 1 , and B6 mice. (a) Results of Western blotting assays showing the expression of serum gp70 in three different strains of mice. Sera were diluted 1:20 into SDS sample buffer without a reducing reagent and boiled for 5 min; 10 μl of each boiled mixture was loaded into a well of 7.5% polyacrylamide gel. Plasma from a 4 month-old female MRL/ lpr ), NZW mice expressed a high level of serum gp85 (gp70 plus p15E), gp70, and a degradation product gp45 (arrowheads), while their expression in B6 mice was low. (BALB/c × MRL/+)F 1 mice (F 1 ) expressed an intermediate level of serum gp70. Mr, biotinylated markers, with positions indicated in kilodaltons at the left. (b to d) Representative photomicrographs taken from kidney sections of NZW (b), (BALB/c × MRL/+)F 1 (c), and B6 (d) mice injected with purified anti-gp70 IgG3, 12H5.1; hematoxylin and eosin staining, ×300. Note apparent thickening of the capillary walls (arrowheads) and inflammatory cell infiltration (arrow) in panel b and marked increase in glomerular cellularity and evident neutrophilic infiltration in panel c.

    Article Snippet: Incubation with MAb and detection of bound Ab by using biotinylated horse anti-mouse Ig secondary Ab and avidin-biotinylated peroxidase complex (Vector Laboratories, Burlingame, CA) has been described elsewhere ( , ).

    Techniques: Expressing, Injection, Purification, Mouse Assay, Western Blot, Staining

    Representative kidney pathology of hybridoma-transplanted mice. (a) (BALB/c × MRL/+)F 1 mouse transplanted with 8.653 fusion partner cells. PAS staining, ×70. (b) (BALB/c × MRL/+)F 1 mouse transplanted with hybridoma cells 12H5.1. PAS staining, ×70. Note the extreme expansion of the glomeruli compared to those in panel a, which shows normal glomeruli at the same magnification. Sizes of tubules and of the nuclei of tubular epithelial cells are not different in panels a and b, but glomeruli are markedly enlarged in panel b. Granular deposition of fibrin in the affected glomeruli was also shown when phosphotungstenic acid-hematoxylin staining was applied (not shown). (c) Immunofluorescence staining with FITC-conjugated anti-mouse IgG of a fresh-frozen section taken from a representative (BALB/c × MRL/+)F 1 mouse transplanted with hybridoma cells 12H5.1. Use of FITC-conjugated anti-mouse C3 resulted in a similar pattern of staining. (d) Electron micrograph showing an affected glomerulus of a representative (BALB/c × MRL/+)F 1 ) was biotinylated to detect the presence of xenotropic viral env gene products. (g) (BALB/c × MRL/+)F 1 mouse transplanted with hybridoma cells 37C4.1 showing typical wire loop lesions. PAS staining, ×175. (h) Electron micrograph of the kidney from a (BALB/c × MRL/+)F 1 mouse transplanted with hybridoma cells 37C4.1. Bar = 2 μm. Note the dense subendothelial deposits consistent with light microscopic wire loops along the basement membrane. (i) SCID mouse transplanted with hybridoma cells 37C4.1. PAS staining, ×140. (j) Dense linear deposition of mouse C3 in a representative glomerulus from a SCID mouse transplanted with hybridoma 37C4.1. Similar deposition of mouse IgG and xenotropic viral gp70 in the affected glomeruli was also demonstrated in fresh-frozen sections of the transplanted SCID mice. (k and l) (BALB/c × MRL/+)F 1 mice transplanted with one clone of hybridomas 51D1.1 and 59C4.1, respectively. PAS staining, ×140. Note PAS-positive deposition and expansion of the mesangial areas in panel k and cell proliferation (arrowheads) and occlusive changes (arrow) of capillaries in panel l. Lesions similar to those in panel l were observed in the mice transplanted with hybridoma 60A5.1 (not shown).

    Journal: Journal of Virology

    Article Title: Establishment of Monoclonal Anti-Retroviral gp70 Autoantibodies from MRL/lpr Lupus Mice and Induction of Glomerular gp70 Deposition and Pathology by Transfer into Non-Autoimmune Mice

    doi:

    Figure Lengend Snippet: Representative kidney pathology of hybridoma-transplanted mice. (a) (BALB/c × MRL/+)F 1 mouse transplanted with 8.653 fusion partner cells. PAS staining, ×70. (b) (BALB/c × MRL/+)F 1 mouse transplanted with hybridoma cells 12H5.1. PAS staining, ×70. Note the extreme expansion of the glomeruli compared to those in panel a, which shows normal glomeruli at the same magnification. Sizes of tubules and of the nuclei of tubular epithelial cells are not different in panels a and b, but glomeruli are markedly enlarged in panel b. Granular deposition of fibrin in the affected glomeruli was also shown when phosphotungstenic acid-hematoxylin staining was applied (not shown). (c) Immunofluorescence staining with FITC-conjugated anti-mouse IgG of a fresh-frozen section taken from a representative (BALB/c × MRL/+)F 1 mouse transplanted with hybridoma cells 12H5.1. Use of FITC-conjugated anti-mouse C3 resulted in a similar pattern of staining. (d) Electron micrograph showing an affected glomerulus of a representative (BALB/c × MRL/+)F 1 ) was biotinylated to detect the presence of xenotropic viral env gene products. (g) (BALB/c × MRL/+)F 1 mouse transplanted with hybridoma cells 37C4.1 showing typical wire loop lesions. PAS staining, ×175. (h) Electron micrograph of the kidney from a (BALB/c × MRL/+)F 1 mouse transplanted with hybridoma cells 37C4.1. Bar = 2 μm. Note the dense subendothelial deposits consistent with light microscopic wire loops along the basement membrane. (i) SCID mouse transplanted with hybridoma cells 37C4.1. PAS staining, ×140. (j) Dense linear deposition of mouse C3 in a representative glomerulus from a SCID mouse transplanted with hybridoma 37C4.1. Similar deposition of mouse IgG and xenotropic viral gp70 in the affected glomeruli was also demonstrated in fresh-frozen sections of the transplanted SCID mice. (k and l) (BALB/c × MRL/+)F 1 mice transplanted with one clone of hybridomas 51D1.1 and 59C4.1, respectively. PAS staining, ×140. Note PAS-positive deposition and expansion of the mesangial areas in panel k and cell proliferation (arrowheads) and occlusive changes (arrow) of capillaries in panel l. Lesions similar to those in panel l were observed in the mice transplanted with hybridoma 60A5.1 (not shown).

    Article Snippet: Incubation with MAb and detection of bound Ab by using biotinylated horse anti-mouse Ig secondary Ab and avidin-biotinylated peroxidase complex (Vector Laboratories, Burlingame, CA) has been described elsewhere ( , ).

    Techniques: Mouse Assay, Staining, Immunofluorescence