biotinylated maa ii  (Vector Laboratories)


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    Vector Laboratories biotinylated maa ii
    Biotinylated Maa Ii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated maa ii lectin  (Vector Laboratories)


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    Vector Laboratories biotinylated maa ii lectin
    Biotinylated Maa Ii Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated maackia amurensis maa  (Vector Laboratories)


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    Vector Laboratories biotinylated maackia amurensis maa
    Biotinylated Maackia Amurensis Maa, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated maa lectin i  (Vector Laboratories)


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    Vector Laboratories biotinylated maa lectin i
    (A) Sialic acids on the cell surface of immortalized human sebocytes (SZ95) were detected by their reaction with <t>biotinylated</t> <t>MAA</t> <t>lectin</t> <t>I</t> (10 µg/ml) and streptavidin-FITC conjugate. FITC-fluorescence intensity was measured by flow cytomtery (FACSCalibur, BD Biosciences, San Jose, CA), reflecting the quantity of sialic acid. The sebocytes were pre-treated with 10 µg/ml of purified recombinant sialidase (green, a), GFP (green, b) or an equal volume of PBS (vehicle) (purple) at pH 6 for 2 h. The decrease in FITC-fluorescence intensity in sialidase-treated sebocytes validated the enzymatic activity of the purified sialidase. (B) After pre-treatment with sialidase, sebocytes were co-cultured with P. acnes (5×10 7 CFU/5×10 6 cells) for 18 h. P. acnes -induced cell death in PBS (vehicle)-, sialidase- or GFP-pretreated sebocytes was assessed by trypan blue. Cell death is presented as the % of dead cells compared to all cultured cells. (C) After washing out unbound P. acnes , the number of P. acnes adhered to sebocytes was calculated by spreading Triton-X (0.01%) lysed sebocytes on agar plates to quantify CFU/cell. (D) The CFU/cell in vehicle-pretreated sebocytes was defined as 100%. Adherence of P. acnes (arrows) to vehicle (a)-, sialidase (b)- or GFP (c)-treated sebocytes was visualized by staining with the Accustain Gram stain kit. Pre-treatment with sialidase significantly increased the adhesion of P. acnes to sebocytes. Shown are representative results of three independent experiments. Error bars are mean±SE ( P <0.01*, P <0.001**, P <0.0005*** by Student's t-test). Bar: 10 µm.
    Biotinylated Maa Lectin I, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Vaccination Targeting a Surface Sialidase of P. acnes: Implication for New Treatment of Acne Vulgaris"

    Article Title: Vaccination Targeting a Surface Sialidase of P. acnes: Implication for New Treatment of Acne Vulgaris

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0001551

    (A) Sialic acids on the cell surface of immortalized human sebocytes (SZ95) were detected by their reaction with biotinylated MAA lectin I (10 µg/ml) and streptavidin-FITC conjugate. FITC-fluorescence intensity was measured by flow cytomtery (FACSCalibur, BD Biosciences, San Jose, CA), reflecting the quantity of sialic acid. The sebocytes were pre-treated with 10 µg/ml of purified recombinant sialidase (green, a), GFP (green, b) or an equal volume of PBS (vehicle) (purple) at pH 6 for 2 h. The decrease in FITC-fluorescence intensity in sialidase-treated sebocytes validated the enzymatic activity of the purified sialidase. (B) After pre-treatment with sialidase, sebocytes were co-cultured with P. acnes (5×10 7 CFU/5×10 6 cells) for 18 h. P. acnes -induced cell death in PBS (vehicle)-, sialidase- or GFP-pretreated sebocytes was assessed by trypan blue. Cell death is presented as the % of dead cells compared to all cultured cells. (C) After washing out unbound P. acnes , the number of P. acnes adhered to sebocytes was calculated by spreading Triton-X (0.01%) lysed sebocytes on agar plates to quantify CFU/cell. (D) The CFU/cell in vehicle-pretreated sebocytes was defined as 100%. Adherence of P. acnes (arrows) to vehicle (a)-, sialidase (b)- or GFP (c)-treated sebocytes was visualized by staining with the Accustain Gram stain kit. Pre-treatment with sialidase significantly increased the adhesion of P. acnes to sebocytes. Shown are representative results of three independent experiments. Error bars are mean±SE ( P <0.01*, P <0.001**, P <0.0005*** by Student's t-test). Bar: 10 µm.
    Figure Legend Snippet: (A) Sialic acids on the cell surface of immortalized human sebocytes (SZ95) were detected by their reaction with biotinylated MAA lectin I (10 µg/ml) and streptavidin-FITC conjugate. FITC-fluorescence intensity was measured by flow cytomtery (FACSCalibur, BD Biosciences, San Jose, CA), reflecting the quantity of sialic acid. The sebocytes were pre-treated with 10 µg/ml of purified recombinant sialidase (green, a), GFP (green, b) or an equal volume of PBS (vehicle) (purple) at pH 6 for 2 h. The decrease in FITC-fluorescence intensity in sialidase-treated sebocytes validated the enzymatic activity of the purified sialidase. (B) After pre-treatment with sialidase, sebocytes were co-cultured with P. acnes (5×10 7 CFU/5×10 6 cells) for 18 h. P. acnes -induced cell death in PBS (vehicle)-, sialidase- or GFP-pretreated sebocytes was assessed by trypan blue. Cell death is presented as the % of dead cells compared to all cultured cells. (C) After washing out unbound P. acnes , the number of P. acnes adhered to sebocytes was calculated by spreading Triton-X (0.01%) lysed sebocytes on agar plates to quantify CFU/cell. (D) The CFU/cell in vehicle-pretreated sebocytes was defined as 100%. Adherence of P. acnes (arrows) to vehicle (a)-, sialidase (b)- or GFP (c)-treated sebocytes was visualized by staining with the Accustain Gram stain kit. Pre-treatment with sialidase significantly increased the adhesion of P. acnes to sebocytes. Shown are representative results of three independent experiments. Error bars are mean±SE ( P <0.01*, P <0.001**, P <0.0005*** by Student's t-test). Bar: 10 µm.

    Techniques Used: Fluorescence, Purification, Recombinant, Activity Assay, Cell Culture, Staining

    biotinylated maackia amurensis lectin ii maa ii  (Vector Laboratories)


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    Vector Laboratories biotinylated maackia amurensis lectin ii maa ii
    Biotinylated Maackia Amurensis Lectin Ii Maa Ii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated maa ii  (Vector Laboratories)


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    Vector Laboratories biotinylated maa ii
    Biotinylated Maa Ii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated maa ii  (Vector Laboratories)


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    Vector Laboratories biotinylated maa ii
    Biotinylated Maa Ii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated maa ii  (Vector Laboratories)


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    Vector Laboratories biotinylated maa ii
    Lectins and antibodies for Flow cytometry (FC), spectral FC, WB, and IHC.
    Biotinylated Maa Ii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Sialyltransferase Inhibitor Ac 5 3F ax Neu5Ac Reverts the Malignant Phenotype of Pancreatic Cancer Cells, and Reduces Tumor Volume and Favors T-Cell Infiltrates in Mice"

    Article Title: Sialyltransferase Inhibitor Ac 5 3F ax Neu5Ac Reverts the Malignant Phenotype of Pancreatic Cancer Cells, and Reduces Tumor Volume and Favors T-Cell Infiltrates in Mice

    Journal: Cancers

    doi: 10.3390/cancers14246133

    Lectins and antibodies for Flow cytometry (FC), spectral FC, WB, and IHC.
    Figure Legend Snippet: Lectins and antibodies for Flow cytometry (FC), spectral FC, WB, and IHC.

    Techniques Used: Flow Cytometry, Plasmid Preparation, Avidin-Biotin Assay

    biotinylated maa ii lectin  (Vector Laboratories)


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    Vector Laboratories biotinylated maa ii lectin
    Differential expression of SAα2,6-Gal (SNA <t>lectin)</t> and SAα2,3-Gal <t>(MAA</t> II lectin) receptors in the porcine respiratory tract . Composite confocal images show distribution of SAα2,6-Gal receptors (green) and SAα2,3-Gal receptors (red) with nuclear staining (blue). Representative results that show SAα2,6-Gal receptor as the dominant receptor type on the epithelium of trachea (A), bronchus (B) and bronchiole (C), where epithelial cells and goblet cells are the main contributing cell types. SAα2,3-Gal receptor, on the other hand, is the major receptor in the corresponding sub-epithelial (mucosal) region with sparse concentration of SAα2,6-Gal receptor at blood vessels and mucous/serous glands. Both receptor types are similarly expressed on alveolar lining (D). The specificity of lectin binding is demonstrated on serial tracheal sections stained with haematoxylin and eosin (E), and with both SNA and MAA II lectins on section previously treated with sialidase A, where only faint background binding is detected (F). 1. goblet cell, 2. epithelial lining, 3. gland with occasional blood vessel, 4. submucosal gland, 5. mucosa, 6. smooth muscle, 7. blood vessel. Scale bar = 75 μm.
    Biotinylated Maa Ii Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Comparative distribution of human and avian type sialic acid influenza receptors in the pig"

    Article Title: Comparative distribution of human and avian type sialic acid influenza receptors in the pig

    Journal: BMC Veterinary Research

    doi: 10.1186/1746-6148-6-4

    Differential expression of SAα2,6-Gal (SNA lectin) and SAα2,3-Gal (MAA II lectin) receptors in the porcine respiratory tract . Composite confocal images show distribution of SAα2,6-Gal receptors (green) and SAα2,3-Gal receptors (red) with nuclear staining (blue). Representative results that show SAα2,6-Gal receptor as the dominant receptor type on the epithelium of trachea (A), bronchus (B) and bronchiole (C), where epithelial cells and goblet cells are the main contributing cell types. SAα2,3-Gal receptor, on the other hand, is the major receptor in the corresponding sub-epithelial (mucosal) region with sparse concentration of SAα2,6-Gal receptor at blood vessels and mucous/serous glands. Both receptor types are similarly expressed on alveolar lining (D). The specificity of lectin binding is demonstrated on serial tracheal sections stained with haematoxylin and eosin (E), and with both SNA and MAA II lectins on section previously treated with sialidase A, where only faint background binding is detected (F). 1. goblet cell, 2. epithelial lining, 3. gland with occasional blood vessel, 4. submucosal gland, 5. mucosa, 6. smooth muscle, 7. blood vessel. Scale bar = 75 μm.
    Figure Legend Snippet: Differential expression of SAα2,6-Gal (SNA lectin) and SAα2,3-Gal (MAA II lectin) receptors in the porcine respiratory tract . Composite confocal images show distribution of SAα2,6-Gal receptors (green) and SAα2,3-Gal receptors (red) with nuclear staining (blue). Representative results that show SAα2,6-Gal receptor as the dominant receptor type on the epithelium of trachea (A), bronchus (B) and bronchiole (C), where epithelial cells and goblet cells are the main contributing cell types. SAα2,3-Gal receptor, on the other hand, is the major receptor in the corresponding sub-epithelial (mucosal) region with sparse concentration of SAα2,6-Gal receptor at blood vessels and mucous/serous glands. Both receptor types are similarly expressed on alveolar lining (D). The specificity of lectin binding is demonstrated on serial tracheal sections stained with haematoxylin and eosin (E), and with both SNA and MAA II lectins on section previously treated with sialidase A, where only faint background binding is detected (F). 1. goblet cell, 2. epithelial lining, 3. gland with occasional blood vessel, 4. submucosal gland, 5. mucosa, 6. smooth muscle, 7. blood vessel. Scale bar = 75 μm.

    Techniques Used: Expressing, Staining, Concentration Assay, Binding Assay

    Discriminating two types of SA α2,3-Gal receptors in bronchiole (A) and colon (B) by FITC labelled MAA I (green), and biotinylated MAA II (red) receptors . In bronchiole, MAA I and MAA II typically show similar binding intensity, with prominent presence of MAA I on the epithelial lining (Aii). In colon, MAA II binding, mainly localised to goblet cells, dominates MAA I; MAA I binding is seen as a fine line bordering the epithelium (Bii). 1. epithelial lining, 2. mucosa, 3. smooth muscle, 4. goblet cell. Scale bar = 75 μm.
    Figure Legend Snippet: Discriminating two types of SA α2,3-Gal receptors in bronchiole (A) and colon (B) by FITC labelled MAA I (green), and biotinylated MAA II (red) receptors . In bronchiole, MAA I and MAA II typically show similar binding intensity, with prominent presence of MAA I on the epithelial lining (Aii). In colon, MAA II binding, mainly localised to goblet cells, dominates MAA I; MAA I binding is seen as a fine line bordering the epithelium (Bii). 1. epithelial lining, 2. mucosa, 3. smooth muscle, 4. goblet cell. Scale bar = 75 μm.

    Techniques Used: Binding Assay

    Differential expression of SAα2,6-Gal (SNA lectin) and SAα2,3-Gal (MAA II lectin) receptors in the porcine intestinal tract . Composite confocal images show distribution of SAα2,6-Gal receptors (green) and SAα2,3-Gal receptors (red) with nuclear staining (blue). Representative results that show the spatial distribution of both receptor types in the duodenum (A) and colon (B, C). In duodenum, SAα2,6-Gal receptor is more abundant than SAα2,3-Gal receptor concentrated in goblet cells and along the epithelial lining (A). In colon, strong co-expression of SAα2,6-Gal and SAα2,3-Gal receptors is detected in goblet cells and on epithelial lining. Colon goblet cells at the crypts show a higher concentration of SAα2,6-Gal receptor (Cii) than those located towards the luminal surface (Bii). 1. epithelial lining, 2. goblet cell. Scale bar = 75 μm.
    Figure Legend Snippet: Differential expression of SAα2,6-Gal (SNA lectin) and SAα2,3-Gal (MAA II lectin) receptors in the porcine intestinal tract . Composite confocal images show distribution of SAα2,6-Gal receptors (green) and SAα2,3-Gal receptors (red) with nuclear staining (blue). Representative results that show the spatial distribution of both receptor types in the duodenum (A) and colon (B, C). In duodenum, SAα2,6-Gal receptor is more abundant than SAα2,3-Gal receptor concentrated in goblet cells and along the epithelial lining (A). In colon, strong co-expression of SAα2,6-Gal and SAα2,3-Gal receptors is detected in goblet cells and on epithelial lining. Colon goblet cells at the crypts show a higher concentration of SAα2,6-Gal receptor (Cii) than those located towards the luminal surface (Bii). 1. epithelial lining, 2. goblet cell. Scale bar = 75 μm.

    Techniques Used: Expressing, Staining, Concentration Assay

    Extensive presence of SAα2,6-Gal (SNA lectin) and SAα2,3-Gal (MAA II lectin) receptors in the major porcine organs examined . Composite confocal images, along with corresponding haematoxylin and eosin tissue sections (with the exception of skeletal muscle) for orientation, show distribution of SAα2,6-Gal receptors (green) and SAα2,3-Gal receptors (red) with nuclear staining (blue). Cross section and longitudinal section of skeletal muscle are shown. 1. hepatic sinusoid, 2. portal triad, 3. meninx, 4. neuron, 5. white pulp, 6. red pulp, 7. capsule, 8. glomerulus, 9. renal tubule, 10. capillary, 11. basement membrane.
    Figure Legend Snippet: Extensive presence of SAα2,6-Gal (SNA lectin) and SAα2,3-Gal (MAA II lectin) receptors in the major porcine organs examined . Composite confocal images, along with corresponding haematoxylin and eosin tissue sections (with the exception of skeletal muscle) for orientation, show distribution of SAα2,6-Gal receptors (green) and SAα2,3-Gal receptors (red) with nuclear staining (blue). Cross section and longitudinal section of skeletal muscle are shown. 1. hepatic sinusoid, 2. portal triad, 3. meninx, 4. neuron, 5. white pulp, 6. red pulp, 7. capsule, 8. glomerulus, 9. renal tubule, 10. capillary, 11. basement membrane.

    Techniques Used: Staining

    Schematic representation of the distribution trend of SAα2,6-Gal (SNA), SAα2,3-Galβ(1-4)GlcNAc (MAA I) and SAα2,3-Galβ(1-3)GalNAc (MAA II) receptors along the porcine respiratory tract . Diagram depicts a qualitative, not quantitative, assessment of receptor presence. Along the epithelial tract, SAα2,6-Gal receptor is dominant, with increasing MAA II lectin binding towards the alveolar region. In the sub-epithelial region, MAA II lectin binding is dominant. MAA I lectin binding is localised to the lower tract.
    Figure Legend Snippet: Schematic representation of the distribution trend of SAα2,6-Gal (SNA), SAα2,3-Galβ(1-4)GlcNAc (MAA I) and SAα2,3-Galβ(1-3)GalNAc (MAA II) receptors along the porcine respiratory tract . Diagram depicts a qualitative, not quantitative, assessment of receptor presence. Along the epithelial tract, SAα2,6-Gal receptor is dominant, with increasing MAA II lectin binding towards the alveolar region. In the sub-epithelial region, MAA II lectin binding is dominant. MAA I lectin binding is localised to the lower tract.

    Techniques Used: Binding Assay

    biotinylated maa lectin  (Vector Laboratories)


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    Vector Laboratories biotinylated maa lectin
    Biotinylated Maa Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories biotinylated maa ii
    Biotinylated Maa Ii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories biotinylated maa ii lectin
    Biotinylated Maa Ii Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories biotinylated maackia amurensis maa
    Biotinylated Maackia Amurensis Maa, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories biotinylated maa lectin i
    (A) Sialic acids on the cell surface of immortalized human sebocytes (SZ95) were detected by their reaction with <t>biotinylated</t> <t>MAA</t> <t>lectin</t> <t>I</t> (10 µg/ml) and streptavidin-FITC conjugate. FITC-fluorescence intensity was measured by flow cytomtery (FACSCalibur, BD Biosciences, San Jose, CA), reflecting the quantity of sialic acid. The sebocytes were pre-treated with 10 µg/ml of purified recombinant sialidase (green, a), GFP (green, b) or an equal volume of PBS (vehicle) (purple) at pH 6 for 2 h. The decrease in FITC-fluorescence intensity in sialidase-treated sebocytes validated the enzymatic activity of the purified sialidase. (B) After pre-treatment with sialidase, sebocytes were co-cultured with P. acnes (5×10 7 CFU/5×10 6 cells) for 18 h. P. acnes -induced cell death in PBS (vehicle)-, sialidase- or GFP-pretreated sebocytes was assessed by trypan blue. Cell death is presented as the % of dead cells compared to all cultured cells. (C) After washing out unbound P. acnes , the number of P. acnes adhered to sebocytes was calculated by spreading Triton-X (0.01%) lysed sebocytes on agar plates to quantify CFU/cell. (D) The CFU/cell in vehicle-pretreated sebocytes was defined as 100%. Adherence of P. acnes (arrows) to vehicle (a)-, sialidase (b)- or GFP (c)-treated sebocytes was visualized by staining with the Accustain Gram stain kit. Pre-treatment with sialidase significantly increased the adhesion of P. acnes to sebocytes. Shown are representative results of three independent experiments. Error bars are mean±SE ( P <0.01*, P <0.001**, P <0.0005*** by Student's t-test). Bar: 10 µm.
    Biotinylated Maa Lectin I, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Vector Laboratories biotinylated maackia amurensis lectin ii maa ii
    (A) Sialic acids on the cell surface of immortalized human sebocytes (SZ95) were detected by their reaction with <t>biotinylated</t> <t>MAA</t> <t>lectin</t> <t>I</t> (10 µg/ml) and streptavidin-FITC conjugate. FITC-fluorescence intensity was measured by flow cytomtery (FACSCalibur, BD Biosciences, San Jose, CA), reflecting the quantity of sialic acid. The sebocytes were pre-treated with 10 µg/ml of purified recombinant sialidase (green, a), GFP (green, b) or an equal volume of PBS (vehicle) (purple) at pH 6 for 2 h. The decrease in FITC-fluorescence intensity in sialidase-treated sebocytes validated the enzymatic activity of the purified sialidase. (B) After pre-treatment with sialidase, sebocytes were co-cultured with P. acnes (5×10 7 CFU/5×10 6 cells) for 18 h. P. acnes -induced cell death in PBS (vehicle)-, sialidase- or GFP-pretreated sebocytes was assessed by trypan blue. Cell death is presented as the % of dead cells compared to all cultured cells. (C) After washing out unbound P. acnes , the number of P. acnes adhered to sebocytes was calculated by spreading Triton-X (0.01%) lysed sebocytes on agar plates to quantify CFU/cell. (D) The CFU/cell in vehicle-pretreated sebocytes was defined as 100%. Adherence of P. acnes (arrows) to vehicle (a)-, sialidase (b)- or GFP (c)-treated sebocytes was visualized by staining with the Accustain Gram stain kit. Pre-treatment with sialidase significantly increased the adhesion of P. acnes to sebocytes. Shown are representative results of three independent experiments. Error bars are mean±SE ( P <0.01*, P <0.001**, P <0.0005*** by Student's t-test). Bar: 10 µm.
    Biotinylated Maackia Amurensis Lectin Ii Maa Ii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Vector Laboratories biotinylated maa lectin
    (A) Sialic acids on the cell surface of immortalized human sebocytes (SZ95) were detected by their reaction with <t>biotinylated</t> <t>MAA</t> <t>lectin</t> <t>I</t> (10 µg/ml) and streptavidin-FITC conjugate. FITC-fluorescence intensity was measured by flow cytomtery (FACSCalibur, BD Biosciences, San Jose, CA), reflecting the quantity of sialic acid. The sebocytes were pre-treated with 10 µg/ml of purified recombinant sialidase (green, a), GFP (green, b) or an equal volume of PBS (vehicle) (purple) at pH 6 for 2 h. The decrease in FITC-fluorescence intensity in sialidase-treated sebocytes validated the enzymatic activity of the purified sialidase. (B) After pre-treatment with sialidase, sebocytes were co-cultured with P. acnes (5×10 7 CFU/5×10 6 cells) for 18 h. P. acnes -induced cell death in PBS (vehicle)-, sialidase- or GFP-pretreated sebocytes was assessed by trypan blue. Cell death is presented as the % of dead cells compared to all cultured cells. (C) After washing out unbound P. acnes , the number of P. acnes adhered to sebocytes was calculated by spreading Triton-X (0.01%) lysed sebocytes on agar plates to quantify CFU/cell. (D) The CFU/cell in vehicle-pretreated sebocytes was defined as 100%. Adherence of P. acnes (arrows) to vehicle (a)-, sialidase (b)- or GFP (c)-treated sebocytes was visualized by staining with the Accustain Gram stain kit. Pre-treatment with sialidase significantly increased the adhesion of P. acnes to sebocytes. Shown are representative results of three independent experiments. Error bars are mean±SE ( P <0.01*, P <0.001**, P <0.0005*** by Student's t-test). Bar: 10 µm.
    Biotinylated Maa Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Price from $9.99 to $1999.99
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    (A) Sialic acids on the cell surface of immortalized human sebocytes (SZ95) were detected by their reaction with biotinylated MAA lectin I (10 µg/ml) and streptavidin-FITC conjugate. FITC-fluorescence intensity was measured by flow cytomtery (FACSCalibur, BD Biosciences, San Jose, CA), reflecting the quantity of sialic acid. The sebocytes were pre-treated with 10 µg/ml of purified recombinant sialidase (green, a), GFP (green, b) or an equal volume of PBS (vehicle) (purple) at pH 6 for 2 h. The decrease in FITC-fluorescence intensity in sialidase-treated sebocytes validated the enzymatic activity of the purified sialidase. (B) After pre-treatment with sialidase, sebocytes were co-cultured with P. acnes (5×10 7 CFU/5×10 6 cells) for 18 h. P. acnes -induced cell death in PBS (vehicle)-, sialidase- or GFP-pretreated sebocytes was assessed by trypan blue. Cell death is presented as the % of dead cells compared to all cultured cells. (C) After washing out unbound P. acnes , the number of P. acnes adhered to sebocytes was calculated by spreading Triton-X (0.01%) lysed sebocytes on agar plates to quantify CFU/cell. (D) The CFU/cell in vehicle-pretreated sebocytes was defined as 100%. Adherence of P. acnes (arrows) to vehicle (a)-, sialidase (b)- or GFP (c)-treated sebocytes was visualized by staining with the Accustain Gram stain kit. Pre-treatment with sialidase significantly increased the adhesion of P. acnes to sebocytes. Shown are representative results of three independent experiments. Error bars are mean±SE ( P <0.01*, P <0.001**, P <0.0005*** by Student's t-test). Bar: 10 µm.

    Journal: PLoS ONE

    Article Title: Vaccination Targeting a Surface Sialidase of P. acnes: Implication for New Treatment of Acne Vulgaris

    doi: 10.1371/journal.pone.0001551

    Figure Lengend Snippet: (A) Sialic acids on the cell surface of immortalized human sebocytes (SZ95) were detected by their reaction with biotinylated MAA lectin I (10 µg/ml) and streptavidin-FITC conjugate. FITC-fluorescence intensity was measured by flow cytomtery (FACSCalibur, BD Biosciences, San Jose, CA), reflecting the quantity of sialic acid. The sebocytes were pre-treated with 10 µg/ml of purified recombinant sialidase (green, a), GFP (green, b) or an equal volume of PBS (vehicle) (purple) at pH 6 for 2 h. The decrease in FITC-fluorescence intensity in sialidase-treated sebocytes validated the enzymatic activity of the purified sialidase. (B) After pre-treatment with sialidase, sebocytes were co-cultured with P. acnes (5×10 7 CFU/5×10 6 cells) for 18 h. P. acnes -induced cell death in PBS (vehicle)-, sialidase- or GFP-pretreated sebocytes was assessed by trypan blue. Cell death is presented as the % of dead cells compared to all cultured cells. (C) After washing out unbound P. acnes , the number of P. acnes adhered to sebocytes was calculated by spreading Triton-X (0.01%) lysed sebocytes on agar plates to quantify CFU/cell. (D) The CFU/cell in vehicle-pretreated sebocytes was defined as 100%. Adherence of P. acnes (arrows) to vehicle (a)-, sialidase (b)- or GFP (c)-treated sebocytes was visualized by staining with the Accustain Gram stain kit. Pre-treatment with sialidase significantly increased the adhesion of P. acnes to sebocytes. Shown are representative results of three independent experiments. Error bars are mean±SE ( P <0.01*, P <0.001**, P <0.0005*** by Student's t-test). Bar: 10 µm.

    Article Snippet: After washing, the cells were incubated at 37°C for 15 min with 10 µg/ml of biotinylated MAA lectin I (Vector Laboratories, Burlingame, CA), which was prepared with 1% (w/v) bovine serum albumin (BSA) in PBS.

    Techniques: Fluorescence, Purification, Recombinant, Activity Assay, Cell Culture, Staining