biotinylated maackia amurensis lectin mal ii  (Vector Laboratories)


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    Vector Laboratories biotinylated maackia amurensis lectin mal ii
    Treatment of human eosinophils with sialidase from V. cholerae selectively cleaves α2,3-linked sialic acids from the cell surface. (A) Human eosinophils isolated from peripheral blood were incubated with <t>biotinylated</t> 1-MDa polyacrylamide decorated with the Siglec-8 glycan ligand 6′-sulfo-3′-sialyl-LacNAc for 20 min at 4°C prior to detection of surface-bound ligand with fluorophore-conjugated streptavidin. Sialidase (Sia) from V. cholerae was used at 50 mU/mL for 1 hr at 37°C to cleave sialic acid from the ligand or from the eosinophils prior to ligand binding. Data are representative (top) or show the quantified normalized Siglec-8 binding to eosinophils relative to control untreated cells as well as overall mean and standard deviations of seven independent experiments (bottom). **, p<0.01; ***, p<0.001; ****, p<0.0001 by one-way ANOVA and Tukey multiple comparisons test. (B, C) Eosinophils were incubated with or without 30 ng/mL rhIL-5 for 18–24 hr. Unprimed cells were then treated with sialidase at the indicated activities for 1 hr prior to detection of cell-surface sialic acids by <t>lectin</t> binding. Surface-bound biotinylated <t>MAL-II</t> (B) or SNA (C) lectin was detected using fluorophore-conjugated streptavidin by flow cytometry. Similarly treated eosinophils incubated without lectin (No Lectin) were analyzed for background streptavidin binding. Data are representative (top) or show quantified normalized lectin binding from three independent experiments (bottom). **, p<0.01; ****, p<0.0001 vs. untreated control sample by two-way ANOVA and Dunnett multiple comparisons test.
    Biotinylated Maackia Amurensis Lectin Mal Ii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Interactions between Siglec-8 and endogenous sialylated cis ligands restrain cell death induction in human eosinophils and mast cells"

    Article Title: Interactions between Siglec-8 and endogenous sialylated cis ligands restrain cell death induction in human eosinophils and mast cells

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2023.1283370

    Treatment of human eosinophils with sialidase from V. cholerae selectively cleaves α2,3-linked sialic acids from the cell surface. (A) Human eosinophils isolated from peripheral blood were incubated with biotinylated 1-MDa polyacrylamide decorated with the Siglec-8 glycan ligand 6′-sulfo-3′-sialyl-LacNAc for 20 min at 4°C prior to detection of surface-bound ligand with fluorophore-conjugated streptavidin. Sialidase (Sia) from V. cholerae was used at 50 mU/mL for 1 hr at 37°C to cleave sialic acid from the ligand or from the eosinophils prior to ligand binding. Data are representative (top) or show the quantified normalized Siglec-8 binding to eosinophils relative to control untreated cells as well as overall mean and standard deviations of seven independent experiments (bottom). **, p<0.01; ***, p<0.001; ****, p<0.0001 by one-way ANOVA and Tukey multiple comparisons test. (B, C) Eosinophils were incubated with or without 30 ng/mL rhIL-5 for 18–24 hr. Unprimed cells were then treated with sialidase at the indicated activities for 1 hr prior to detection of cell-surface sialic acids by lectin binding. Surface-bound biotinylated MAL-II (B) or SNA (C) lectin was detected using fluorophore-conjugated streptavidin by flow cytometry. Similarly treated eosinophils incubated without lectin (No Lectin) were analyzed for background streptavidin binding. Data are representative (top) or show quantified normalized lectin binding from three independent experiments (bottom). **, p<0.01; ****, p<0.0001 vs. untreated control sample by two-way ANOVA and Dunnett multiple comparisons test.
    Figure Legend Snippet: Treatment of human eosinophils with sialidase from V. cholerae selectively cleaves α2,3-linked sialic acids from the cell surface. (A) Human eosinophils isolated from peripheral blood were incubated with biotinylated 1-MDa polyacrylamide decorated with the Siglec-8 glycan ligand 6′-sulfo-3′-sialyl-LacNAc for 20 min at 4°C prior to detection of surface-bound ligand with fluorophore-conjugated streptavidin. Sialidase (Sia) from V. cholerae was used at 50 mU/mL for 1 hr at 37°C to cleave sialic acid from the ligand or from the eosinophils prior to ligand binding. Data are representative (top) or show the quantified normalized Siglec-8 binding to eosinophils relative to control untreated cells as well as overall mean and standard deviations of seven independent experiments (bottom). **, p<0.01; ***, p<0.001; ****, p<0.0001 by one-way ANOVA and Tukey multiple comparisons test. (B, C) Eosinophils were incubated with or without 30 ng/mL rhIL-5 for 18–24 hr. Unprimed cells were then treated with sialidase at the indicated activities for 1 hr prior to detection of cell-surface sialic acids by lectin binding. Surface-bound biotinylated MAL-II (B) or SNA (C) lectin was detected using fluorophore-conjugated streptavidin by flow cytometry. Similarly treated eosinophils incubated without lectin (No Lectin) were analyzed for background streptavidin binding. Data are representative (top) or show quantified normalized lectin binding from three independent experiments (bottom). **, p<0.01; ****, p<0.0001 vs. untreated control sample by two-way ANOVA and Dunnett multiple comparisons test.

    Techniques Used: Isolation, Incubation, Ligand Binding Assay, Binding Assay, Flow Cytometry

    biotinylated maackia amurensis lectin mal ii  (Vector Laboratories)


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    Vector Laboratories biotinylated maackia amurensis lectin mal ii
    Biotinylated Maackia Amurensis Lectin Mal Ii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated maackia amurensis mal ii lectin  (Vector Laboratories)


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    Vector Laboratories biotinylated maackia amurensis mal ii lectin
    Biotinylated Maackia Amurensis Mal Ii Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pbs  (Vector Laboratories)


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    b 1265 1  (Vector Laboratories)


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    Vector Laboratories b 1265 1
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    biotinylated maackia amurensis mal ii lectin  (Vector Laboratories)


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    Vector Laboratories biotinylated maackia amurensis mal ii lectin
    Biotinylated Maackia Amurensis Mal Ii Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated maackia amurensis agglutinin ii  (Vector Laboratories)


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    Vector Laboratories biotinylated maackia amurensis agglutinin ii
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    biotinylated maa ii  (Vector Laboratories)


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    Vector Laboratories biotinylated maa ii
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    biotinylated maackia amurensis lectin  (Vector Laboratories)


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    Vector Laboratories biotinylated maackia amurensis lectin
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    biotinylated maackia amurensis lectin ii maaii  (Vector Laboratories)


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    Vector Laboratories biotinylated maackia amurensis lectin ii maaii
    Sialic acids were detected by <t>lectin</t> staining: MAA ( <t>Maackia</t> <t>amurensis</t> agglutinin ) for α2,3-linked sialic acids and SNA ( Sambucus nigra agglutinin ) for α2,6-linked sialic acids. Ciliated cells were stained using an anti-β-tubulin antibody and mucus-producing cells were stained using an anti muc5ac antibody. In panels A, lectin-staining is compared with staining of ciliated (red) or mucus-producing cells (red in Ab, green in Ad); SNA staining is shown in green (Aa and Ab), MAA staining is shown in green (Ac) or red (Ad). In Ba, co-staining of ciliated (red) and mucus-producing cells (green) is shown; Bb shows co-staining with MAA (red) and SNA (green). In C samples were co-stained SNA (green) and MAA (red); cryosections were derived from PCLS before (Ca) or after neuraminidase treatment (Cb).
    Biotinylated Maackia Amurensis Lectin Ii Maaii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Infection of Differentiated Porcine Airway Epithelial Cells by Influenza Virus: Differential Susceptibility to Infection by Porcine and Avian Viruses"

    Article Title: Infection of Differentiated Porcine Airway Epithelial Cells by Influenza Virus: Differential Susceptibility to Infection by Porcine and Avian Viruses

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0028429

    Sialic acids were detected by lectin staining: MAA ( Maackia amurensis agglutinin ) for α2,3-linked sialic acids and SNA ( Sambucus nigra agglutinin ) for α2,6-linked sialic acids. Ciliated cells were stained using an anti-β-tubulin antibody and mucus-producing cells were stained using an anti muc5ac antibody. In panels A, lectin-staining is compared with staining of ciliated (red) or mucus-producing cells (red in Ab, green in Ad); SNA staining is shown in green (Aa and Ab), MAA staining is shown in green (Ac) or red (Ad). In Ba, co-staining of ciliated (red) and mucus-producing cells (green) is shown; Bb shows co-staining with MAA (red) and SNA (green). In C samples were co-stained SNA (green) and MAA (red); cryosections were derived from PCLS before (Ca) or after neuraminidase treatment (Cb).
    Figure Legend Snippet: Sialic acids were detected by lectin staining: MAA ( Maackia amurensis agglutinin ) for α2,3-linked sialic acids and SNA ( Sambucus nigra agglutinin ) for α2,6-linked sialic acids. Ciliated cells were stained using an anti-β-tubulin antibody and mucus-producing cells were stained using an anti muc5ac antibody. In panels A, lectin-staining is compared with staining of ciliated (red) or mucus-producing cells (red in Ab, green in Ad); SNA staining is shown in green (Aa and Ab), MAA staining is shown in green (Ac) or red (Ad). In Ba, co-staining of ciliated (red) and mucus-producing cells (green) is shown; Bb shows co-staining with MAA (red) and SNA (green). In C samples were co-stained SNA (green) and MAA (red); cryosections were derived from PCLS before (Ca) or after neuraminidase treatment (Cb).

    Techniques Used: Staining, Derivative Assay

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    Vector Laboratories biotinylated maackia amurensis lectin mal ii
    Treatment of human eosinophils with sialidase from V. cholerae selectively cleaves α2,3-linked sialic acids from the cell surface. (A) Human eosinophils isolated from peripheral blood were incubated with <t>biotinylated</t> 1-MDa polyacrylamide decorated with the Siglec-8 glycan ligand 6′-sulfo-3′-sialyl-LacNAc for 20 min at 4°C prior to detection of surface-bound ligand with fluorophore-conjugated streptavidin. Sialidase (Sia) from V. cholerae was used at 50 mU/mL for 1 hr at 37°C to cleave sialic acid from the ligand or from the eosinophils prior to ligand binding. Data are representative (top) or show the quantified normalized Siglec-8 binding to eosinophils relative to control untreated cells as well as overall mean and standard deviations of seven independent experiments (bottom). **, p<0.01; ***, p<0.001; ****, p<0.0001 by one-way ANOVA and Tukey multiple comparisons test. (B, C) Eosinophils were incubated with or without 30 ng/mL rhIL-5 for 18–24 hr. Unprimed cells were then treated with sialidase at the indicated activities for 1 hr prior to detection of cell-surface sialic acids by <t>lectin</t> binding. Surface-bound biotinylated <t>MAL-II</t> (B) or SNA (C) lectin was detected using fluorophore-conjugated streptavidin by flow cytometry. Similarly treated eosinophils incubated without lectin (No Lectin) were analyzed for background streptavidin binding. Data are representative (top) or show quantified normalized lectin binding from three independent experiments (bottom). **, p<0.01; ****, p<0.0001 vs. untreated control sample by two-way ANOVA and Dunnett multiple comparisons test.
    Biotinylated Maackia Amurensis Lectin Mal Ii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories biotinylated maackia amurensis mal ii lectin
    Treatment of human eosinophils with sialidase from V. cholerae selectively cleaves α2,3-linked sialic acids from the cell surface. (A) Human eosinophils isolated from peripheral blood were incubated with <t>biotinylated</t> 1-MDa polyacrylamide decorated with the Siglec-8 glycan ligand 6′-sulfo-3′-sialyl-LacNAc for 20 min at 4°C prior to detection of surface-bound ligand with fluorophore-conjugated streptavidin. Sialidase (Sia) from V. cholerae was used at 50 mU/mL for 1 hr at 37°C to cleave sialic acid from the ligand or from the eosinophils prior to ligand binding. Data are representative (top) or show the quantified normalized Siglec-8 binding to eosinophils relative to control untreated cells as well as overall mean and standard deviations of seven independent experiments (bottom). **, p<0.01; ***, p<0.001; ****, p<0.0001 by one-way ANOVA and Tukey multiple comparisons test. (B, C) Eosinophils were incubated with or without 30 ng/mL rhIL-5 for 18–24 hr. Unprimed cells were then treated with sialidase at the indicated activities for 1 hr prior to detection of cell-surface sialic acids by <t>lectin</t> binding. Surface-bound biotinylated <t>MAL-II</t> (B) or SNA (C) lectin was detected using fluorophore-conjugated streptavidin by flow cytometry. Similarly treated eosinophils incubated without lectin (No Lectin) were analyzed for background streptavidin binding. Data are representative (top) or show quantified normalized lectin binding from three independent experiments (bottom). **, p<0.01; ****, p<0.0001 vs. untreated control sample by two-way ANOVA and Dunnett multiple comparisons test.
    Biotinylated Maackia Amurensis Mal Ii Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories pbs
    Treatment of human eosinophils with sialidase from V. cholerae selectively cleaves α2,3-linked sialic acids from the cell surface. (A) Human eosinophils isolated from peripheral blood were incubated with <t>biotinylated</t> 1-MDa polyacrylamide decorated with the Siglec-8 glycan ligand 6′-sulfo-3′-sialyl-LacNAc for 20 min at 4°C prior to detection of surface-bound ligand with fluorophore-conjugated streptavidin. Sialidase (Sia) from V. cholerae was used at 50 mU/mL for 1 hr at 37°C to cleave sialic acid from the ligand or from the eosinophils prior to ligand binding. Data are representative (top) or show the quantified normalized Siglec-8 binding to eosinophils relative to control untreated cells as well as overall mean and standard deviations of seven independent experiments (bottom). **, p<0.01; ***, p<0.001; ****, p<0.0001 by one-way ANOVA and Tukey multiple comparisons test. (B, C) Eosinophils were incubated with or without 30 ng/mL rhIL-5 for 18–24 hr. Unprimed cells were then treated with sialidase at the indicated activities for 1 hr prior to detection of cell-surface sialic acids by <t>lectin</t> binding. Surface-bound biotinylated <t>MAL-II</t> (B) or SNA (C) lectin was detected using fluorophore-conjugated streptavidin by flow cytometry. Similarly treated eosinophils incubated without lectin (No Lectin) were analyzed for background streptavidin binding. Data are representative (top) or show quantified normalized lectin binding from three independent experiments (bottom). **, p<0.01; ****, p<0.0001 vs. untreated control sample by two-way ANOVA and Dunnett multiple comparisons test.
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    Vector Laboratories b 1265 1
    Treatment of human eosinophils with sialidase from V. cholerae selectively cleaves α2,3-linked sialic acids from the cell surface. (A) Human eosinophils isolated from peripheral blood were incubated with <t>biotinylated</t> 1-MDa polyacrylamide decorated with the Siglec-8 glycan ligand 6′-sulfo-3′-sialyl-LacNAc for 20 min at 4°C prior to detection of surface-bound ligand with fluorophore-conjugated streptavidin. Sialidase (Sia) from V. cholerae was used at 50 mU/mL for 1 hr at 37°C to cleave sialic acid from the ligand or from the eosinophils prior to ligand binding. Data are representative (top) or show the quantified normalized Siglec-8 binding to eosinophils relative to control untreated cells as well as overall mean and standard deviations of seven independent experiments (bottom). **, p<0.01; ***, p<0.001; ****, p<0.0001 by one-way ANOVA and Tukey multiple comparisons test. (B, C) Eosinophils were incubated with or without 30 ng/mL rhIL-5 for 18–24 hr. Unprimed cells were then treated with sialidase at the indicated activities for 1 hr prior to detection of cell-surface sialic acids by <t>lectin</t> binding. Surface-bound biotinylated <t>MAL-II</t> (B) or SNA (C) lectin was detected using fluorophore-conjugated streptavidin by flow cytometry. Similarly treated eosinophils incubated without lectin (No Lectin) were analyzed for background streptavidin binding. Data are representative (top) or show quantified normalized lectin binding from three independent experiments (bottom). **, p<0.01; ****, p<0.0001 vs. untreated control sample by two-way ANOVA and Dunnett multiple comparisons test.
    B 1265 1, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories biotinylated maackia amurensis agglutinin ii
    Treatment of human eosinophils with sialidase from V. cholerae selectively cleaves α2,3-linked sialic acids from the cell surface. (A) Human eosinophils isolated from peripheral blood were incubated with <t>biotinylated</t> 1-MDa polyacrylamide decorated with the Siglec-8 glycan ligand 6′-sulfo-3′-sialyl-LacNAc for 20 min at 4°C prior to detection of surface-bound ligand with fluorophore-conjugated streptavidin. Sialidase (Sia) from V. cholerae was used at 50 mU/mL for 1 hr at 37°C to cleave sialic acid from the ligand or from the eosinophils prior to ligand binding. Data are representative (top) or show the quantified normalized Siglec-8 binding to eosinophils relative to control untreated cells as well as overall mean and standard deviations of seven independent experiments (bottom). **, p<0.01; ***, p<0.001; ****, p<0.0001 by one-way ANOVA and Tukey multiple comparisons test. (B, C) Eosinophils were incubated with or without 30 ng/mL rhIL-5 for 18–24 hr. Unprimed cells were then treated with sialidase at the indicated activities for 1 hr prior to detection of cell-surface sialic acids by <t>lectin</t> binding. Surface-bound biotinylated <t>MAL-II</t> (B) or SNA (C) lectin was detected using fluorophore-conjugated streptavidin by flow cytometry. Similarly treated eosinophils incubated without lectin (No Lectin) were analyzed for background streptavidin binding. Data are representative (top) or show quantified normalized lectin binding from three independent experiments (bottom). **, p<0.01; ****, p<0.0001 vs. untreated control sample by two-way ANOVA and Dunnett multiple comparisons test.
    Biotinylated Maackia Amurensis Agglutinin Ii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Vector Laboratories biotinylated maa ii
    Treatment of human eosinophils with sialidase from V. cholerae selectively cleaves α2,3-linked sialic acids from the cell surface. (A) Human eosinophils isolated from peripheral blood were incubated with <t>biotinylated</t> 1-MDa polyacrylamide decorated with the Siglec-8 glycan ligand 6′-sulfo-3′-sialyl-LacNAc for 20 min at 4°C prior to detection of surface-bound ligand with fluorophore-conjugated streptavidin. Sialidase (Sia) from V. cholerae was used at 50 mU/mL for 1 hr at 37°C to cleave sialic acid from the ligand or from the eosinophils prior to ligand binding. Data are representative (top) or show the quantified normalized Siglec-8 binding to eosinophils relative to control untreated cells as well as overall mean and standard deviations of seven independent experiments (bottom). **, p<0.01; ***, p<0.001; ****, p<0.0001 by one-way ANOVA and Tukey multiple comparisons test. (B, C) Eosinophils were incubated with or without 30 ng/mL rhIL-5 for 18–24 hr. Unprimed cells were then treated with sialidase at the indicated activities for 1 hr prior to detection of cell-surface sialic acids by <t>lectin</t> binding. Surface-bound biotinylated <t>MAL-II</t> (B) or SNA (C) lectin was detected using fluorophore-conjugated streptavidin by flow cytometry. Similarly treated eosinophils incubated without lectin (No Lectin) were analyzed for background streptavidin binding. Data are representative (top) or show quantified normalized lectin binding from three independent experiments (bottom). **, p<0.01; ****, p<0.0001 vs. untreated control sample by two-way ANOVA and Dunnett multiple comparisons test.
    Biotinylated Maa Ii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Vector Laboratories biotinylated maackia amurensis lectin
    Treatment of human eosinophils with sialidase from V. cholerae selectively cleaves α2,3-linked sialic acids from the cell surface. (A) Human eosinophils isolated from peripheral blood were incubated with <t>biotinylated</t> 1-MDa polyacrylamide decorated with the Siglec-8 glycan ligand 6′-sulfo-3′-sialyl-LacNAc for 20 min at 4°C prior to detection of surface-bound ligand with fluorophore-conjugated streptavidin. Sialidase (Sia) from V. cholerae was used at 50 mU/mL for 1 hr at 37°C to cleave sialic acid from the ligand or from the eosinophils prior to ligand binding. Data are representative (top) or show the quantified normalized Siglec-8 binding to eosinophils relative to control untreated cells as well as overall mean and standard deviations of seven independent experiments (bottom). **, p<0.01; ***, p<0.001; ****, p<0.0001 by one-way ANOVA and Tukey multiple comparisons test. (B, C) Eosinophils were incubated with or without 30 ng/mL rhIL-5 for 18–24 hr. Unprimed cells were then treated with sialidase at the indicated activities for 1 hr prior to detection of cell-surface sialic acids by <t>lectin</t> binding. Surface-bound biotinylated <t>MAL-II</t> (B) or SNA (C) lectin was detected using fluorophore-conjugated streptavidin by flow cytometry. Similarly treated eosinophils incubated without lectin (No Lectin) were analyzed for background streptavidin binding. Data are representative (top) or show quantified normalized lectin binding from three independent experiments (bottom). **, p<0.01; ****, p<0.0001 vs. untreated control sample by two-way ANOVA and Dunnett multiple comparisons test.
    Biotinylated Maackia Amurensis Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated maackia amurensis lectin/product/Vector Laboratories
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated maackia amurensis lectin - by Bioz Stars, 2024-06
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    95
    Vector Laboratories biotinylated maackia amurensis lectin ii maaii
    Sialic acids were detected by <t>lectin</t> staining: MAA ( <t>Maackia</t> <t>amurensis</t> agglutinin ) for α2,3-linked sialic acids and SNA ( Sambucus nigra agglutinin ) for α2,6-linked sialic acids. Ciliated cells were stained using an anti-β-tubulin antibody and mucus-producing cells were stained using an anti muc5ac antibody. In panels A, lectin-staining is compared with staining of ciliated (red) or mucus-producing cells (red in Ab, green in Ad); SNA staining is shown in green (Aa and Ab), MAA staining is shown in green (Ac) or red (Ad). In Ba, co-staining of ciliated (red) and mucus-producing cells (green) is shown; Bb shows co-staining with MAA (red) and SNA (green). In C samples were co-stained SNA (green) and MAA (red); cryosections were derived from PCLS before (Ca) or after neuraminidase treatment (Cb).
    Biotinylated Maackia Amurensis Lectin Ii Maaii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated maackia amurensis lectin ii maaii/product/Vector Laboratories
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated maackia amurensis lectin ii maaii - by Bioz Stars, 2024-06
    95/100 stars
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    Treatment of human eosinophils with sialidase from V. cholerae selectively cleaves α2,3-linked sialic acids from the cell surface. (A) Human eosinophils isolated from peripheral blood were incubated with biotinylated 1-MDa polyacrylamide decorated with the Siglec-8 glycan ligand 6′-sulfo-3′-sialyl-LacNAc for 20 min at 4°C prior to detection of surface-bound ligand with fluorophore-conjugated streptavidin. Sialidase (Sia) from V. cholerae was used at 50 mU/mL for 1 hr at 37°C to cleave sialic acid from the ligand or from the eosinophils prior to ligand binding. Data are representative (top) or show the quantified normalized Siglec-8 binding to eosinophils relative to control untreated cells as well as overall mean and standard deviations of seven independent experiments (bottom). **, p<0.01; ***, p<0.001; ****, p<0.0001 by one-way ANOVA and Tukey multiple comparisons test. (B, C) Eosinophils were incubated with or without 30 ng/mL rhIL-5 for 18–24 hr. Unprimed cells were then treated with sialidase at the indicated activities for 1 hr prior to detection of cell-surface sialic acids by lectin binding. Surface-bound biotinylated MAL-II (B) or SNA (C) lectin was detected using fluorophore-conjugated streptavidin by flow cytometry. Similarly treated eosinophils incubated without lectin (No Lectin) were analyzed for background streptavidin binding. Data are representative (top) or show quantified normalized lectin binding from three independent experiments (bottom). **, p<0.01; ****, p<0.0001 vs. untreated control sample by two-way ANOVA and Dunnett multiple comparisons test.

    Journal: Frontiers in Immunology

    Article Title: Interactions between Siglec-8 and endogenous sialylated cis ligands restrain cell death induction in human eosinophils and mast cells

    doi: 10.3389/fimmu.2023.1283370

    Figure Lengend Snippet: Treatment of human eosinophils with sialidase from V. cholerae selectively cleaves α2,3-linked sialic acids from the cell surface. (A) Human eosinophils isolated from peripheral blood were incubated with biotinylated 1-MDa polyacrylamide decorated with the Siglec-8 glycan ligand 6′-sulfo-3′-sialyl-LacNAc for 20 min at 4°C prior to detection of surface-bound ligand with fluorophore-conjugated streptavidin. Sialidase (Sia) from V. cholerae was used at 50 mU/mL for 1 hr at 37°C to cleave sialic acid from the ligand or from the eosinophils prior to ligand binding. Data are representative (top) or show the quantified normalized Siglec-8 binding to eosinophils relative to control untreated cells as well as overall mean and standard deviations of seven independent experiments (bottom). **, p<0.01; ***, p<0.001; ****, p<0.0001 by one-way ANOVA and Tukey multiple comparisons test. (B, C) Eosinophils were incubated with or without 30 ng/mL rhIL-5 for 18–24 hr. Unprimed cells were then treated with sialidase at the indicated activities for 1 hr prior to detection of cell-surface sialic acids by lectin binding. Surface-bound biotinylated MAL-II (B) or SNA (C) lectin was detected using fluorophore-conjugated streptavidin by flow cytometry. Similarly treated eosinophils incubated without lectin (No Lectin) were analyzed for background streptavidin binding. Data are representative (top) or show quantified normalized lectin binding from three independent experiments (bottom). **, p<0.01; ****, p<0.0001 vs. untreated control sample by two-way ANOVA and Dunnett multiple comparisons test.

    Article Snippet: To detect cell-surface sialic acid, eosinophils (2×10 5 cells per condition) were incubated with biotinylated Maackia amurensis lectin (MAL)-II or Sambucus nigra agglutinin (SNA) (both from Vector Laboratories, Newark, CA) at 10 μg/mL for 30 min. After washing, cell surface-bound lectin was stained with DyLight488-conjugated streptavidin (Vector Laboratories) at 10 μg/mL for 30 min and detected by flow cytometry.

    Techniques: Isolation, Incubation, Ligand Binding Assay, Binding Assay, Flow Cytometry

    Sialic acids were detected by lectin staining: MAA ( Maackia amurensis agglutinin ) for α2,3-linked sialic acids and SNA ( Sambucus nigra agglutinin ) for α2,6-linked sialic acids. Ciliated cells were stained using an anti-β-tubulin antibody and mucus-producing cells were stained using an anti muc5ac antibody. In panels A, lectin-staining is compared with staining of ciliated (red) or mucus-producing cells (red in Ab, green in Ad); SNA staining is shown in green (Aa and Ab), MAA staining is shown in green (Ac) or red (Ad). In Ba, co-staining of ciliated (red) and mucus-producing cells (green) is shown; Bb shows co-staining with MAA (red) and SNA (green). In C samples were co-stained SNA (green) and MAA (red); cryosections were derived from PCLS before (Ca) or after neuraminidase treatment (Cb).

    Journal: PLoS ONE

    Article Title: Infection of Differentiated Porcine Airway Epithelial Cells by Influenza Virus: Differential Susceptibility to Infection by Porcine and Avian Viruses

    doi: 10.1371/journal.pone.0028429

    Figure Lengend Snippet: Sialic acids were detected by lectin staining: MAA ( Maackia amurensis agglutinin ) for α2,3-linked sialic acids and SNA ( Sambucus nigra agglutinin ) for α2,6-linked sialic acids. Ciliated cells were stained using an anti-β-tubulin antibody and mucus-producing cells were stained using an anti muc5ac antibody. In panels A, lectin-staining is compared with staining of ciliated (red) or mucus-producing cells (red in Ab, green in Ad); SNA staining is shown in green (Aa and Ab), MAA staining is shown in green (Ac) or red (Ad). In Ba, co-staining of ciliated (red) and mucus-producing cells (green) is shown; Bb shows co-staining with MAA (red) and SNA (green). In C samples were co-stained SNA (green) and MAA (red); cryosections were derived from PCLS before (Ca) or after neuraminidase treatment (Cb).

    Article Snippet: To detect α2,6-linked sialic acids, FITC labeled Sambucus nigra agglutinin (SNA) (Vector laboratories, Burlungame, USA) was used and biotinylated Maackia amurensis lectin II (MAAII) was used to determine α2,3-linked sialic acids after preincubation of sections with the Avidin/Biotin Blocking kit (both from Vector Laboratories, USA).

    Techniques: Staining, Derivative Assay