Biotinylated Leucoagglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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1) Product Images from "Sorafenib induced alteration of protein glycosylation in hepatocellular carcinoma cells"
Article Title: Sorafenib induced alteration of protein glycosylation in hepatocellular carcinoma cells
Journal: Oncology Letters
Figure Legend Snippet: Results of lectin blotting and western blotting. (A) Lectin blotting validated Sorafenib induced alteration by the biotinylated MAL-I and biotinylated PHA-L. Anti-β-actin antibody was used to show the protein loading. (B) Downregulation of p-ERK in sorafenib-treated MHCC97L and MHCC97H cells. (C) Downregulation of p-ERK in MHCC97L and MHCC97H cells with U0126 treatment. (D) Diagram of the possible mechanism by which sorafenib treatment reduced the expression of Ets-1. sorafenib treatment decreased the expression of Ets-1 by blocking the Ras/Raf/MAPK signaling pathway in HCC cells. MAL-1, Maackia amurensis lecin I; PHA-L, Phaseolus vulgaris leucoagglutinin; p-, phosphorylated; MEK, mitogen activated protein kinase; ERK, extracellular signal-related kinases; Ets-1, erythroblastosis-26; U0126, 1,4-Diamino-2,3-dicyano-1,4-bis (2-aminophenylthio) butadiene.
Techniques Used: Western Blot, Expressing, Blocking Assay