biotinylated horse anti mouse secondary antibody  (Vector Laboratories)


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    Name:
    Biotinylated Horse Anti Mouse IgG Antibody
    Description:
    Biotinylated Horse Anti Mouse IgG Antibodyd is prepared using proprietary immunization schedules that produce high affinity antibodies The antibodies are then purified by affinity chromatography and cross reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining ELISAs and blots Biotinylated Horse Anti Mouse IgG H L is supplied in solution With some exceptions the recommended dilution for most applications is 1 200 H L indicates the antibody recognizes both heavy and light chains This antibody is included in the VECTASTAIN ABC kits
    Catalog Number:
    ba-2000
    Price:
    None
    Category:
    Antibodies
    Reactivity:
    Mouse
    Size:
    1 5 mg
    Host:
    Horse
    		Buy from Supplier

    Structured Review

    Vector Laboratories biotinylated horse anti mouse secondary antibody
    Biotinylated Horse Anti Mouse IgG Antibody
    Biotinylated Horse Anti Mouse IgG Antibodyd is prepared using proprietary immunization schedules that produce high affinity antibodies The antibodies are then purified by affinity chromatography and cross reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining ELISAs and blots Biotinylated Horse Anti Mouse IgG H L is supplied in solution With some exceptions the recommended dilution for most applications is 1 200 H L indicates the antibody recognizes both heavy and light chains This antibody is included in the VECTASTAIN ABC kits
    https://www.bioz.com/result/biotinylated horse anti mouse secondary antibody/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated horse anti mouse secondary antibody - by Bioz Stars, 2021-03
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: ADAM12 Is Selectively Overexpressed in Human Glioblastomas and Is Associated with Glioblastoma Cell Proliferation and Shedding of Heparin-Binding Epidermal Growth Factor
    Article Snippet: After blocking nonspecific binding with 10% horse serum for ADAM12m staining or 10% goat serum for Ki-67 staining, they were incubated with mouse monoclonal antibodies against ADAM12m (283-6H3, 5 μg/ml) or Ki-67 (MIB1, 1/50 dilution; DakoCytomation Norden A/S). .. Subsequently, the specimens were incubated with biotinylated horse antibodies against mouse IgG (1/200 dilution; Vector Laboratories, Inc., Burlingame, CA) followed by the ABC method for ADAM12m or with goat antibodies against mouse IgG conjugated to horseradish peroxidase-labeled dextran polymer (no dilution, EnVision+ Peroxidase Mouse; DakoCytomation, California Inc., Carpinteria, CA) for Ki-67. ..

    Article Title: Establishment of Monoclonal Anti-Retroviral gp70 Autoantibodies from MRL/lpr Lupus Mice and Induction of Glomerular gp70 Deposition and Pathology by Transfer into Non-Autoimmune Mice
    Article Snippet: Proteins separated through 7.5% polyacrylamide gels were transferred onto polyvinylidene difluoride membranes (Immobilon; Millipore Corporation, Bedford, Mass.) as described previously ( , ), and the blotted membrane was blocked with 10% skim milk. .. Incubation with MAb and detection of bound Ab by using biotinylated horse anti-mouse Ig secondary Ab and avidin-biotinylated peroxidase complex (Vector Laboratories, Burlingame, CA) has been described elsewhere ( , ). .. For the detection of serum gp70, sera from NZW, (BALB/c × MRL/+)F1 , and B6 mice were mixed at 1:20 with the SDS sample buffer containing no reducing agent, and serum proteins were separated through 7.5% polyacrylamide gels and blotted as described above.

    Article Title: Novel Chlamydia muridarum T cell Antigens Induce Protective Immunity against Lung and Genital Tract Infection in Murine Models
    Article Snippet: All anti- Chlamydia recombinant protein polyclonal antibodies had titers ≥1: 500,000 dilution as determined by ELISA. .. Biotinylated horse anti-mouse IgG (1: 2000) (Vector Laboratories) was added and then the cells were incubated again for 1 h. Finally, the cells were incubated for 45 min with ABC Reagent (Vector Laboratories) and incubated with peroxidase substrate solution (DAB substrate kit SK-4100; Vector Laboratories) until the desired stain intensity developed. ..

    Article Title: A novel multiplex assay for simultaneous quantification of total and S129 phosphorylated human alpha-synuclein
    Article Snippet: .. The next day sections were rinsed with TBS-T and incubated with horse anti mouse biotinylated secondary antibody (1:200, Vector Laboratories Inc.) in 1 % BSA in TBS-T for 1 h. Sections were again washed with TBS-T and incubated with an avidin-biotin-peroxidase complex solution (Vectastain ABC kit, Vector Laboratories Inc., USA) for 1 h. The specific staining was visualized using 3,3′-diaminobenzidine (DAB Safe, Saveen Werner, Sweden) and 0.01 % H2 O2 . .. For preservation and visualization, sections were mounted on chromatin-gelatin coated glass slides, dried, dehydrated in increasing alcohol solutions, cleared in xylene and coverslipped using DPX (Sigma-Aldrich, Sweden).

    Article Title: Fibroblast Heterogeneity
    Article Snippet: An isotype control antibody mouse IgG1 (Caltag, Burlingame, CA) was included at 10 μg/ml. .. Sections were then incubated with biotinylated horse anti-mouse IgG (Vector Laboratories) and the avidin-biotin-peroxidase detection system was used (Elite ABC 6101; Vector Laboratories). .. Immunofluorescence was used to determine Thy 1 expression in the myometrial and endometrial fibroblast strains.

    Avidin-Biotin Assay:

    Article Title: Establishment of Monoclonal Anti-Retroviral gp70 Autoantibodies from MRL/lpr Lupus Mice and Induction of Glomerular gp70 Deposition and Pathology by Transfer into Non-Autoimmune Mice
    Article Snippet: Proteins separated through 7.5% polyacrylamide gels were transferred onto polyvinylidene difluoride membranes (Immobilon; Millipore Corporation, Bedford, Mass.) as described previously ( , ), and the blotted membrane was blocked with 10% skim milk. .. Incubation with MAb and detection of bound Ab by using biotinylated horse anti-mouse Ig secondary Ab and avidin-biotinylated peroxidase complex (Vector Laboratories, Burlingame, CA) has been described elsewhere ( , ). .. For the detection of serum gp70, sera from NZW, (BALB/c × MRL/+)F1 , and B6 mice were mixed at 1:20 with the SDS sample buffer containing no reducing agent, and serum proteins were separated through 7.5% polyacrylamide gels and blotted as described above.

    Article Title: A novel multiplex assay for simultaneous quantification of total and S129 phosphorylated human alpha-synuclein
    Article Snippet: .. The next day sections were rinsed with TBS-T and incubated with horse anti mouse biotinylated secondary antibody (1:200, Vector Laboratories Inc.) in 1 % BSA in TBS-T for 1 h. Sections were again washed with TBS-T and incubated with an avidin-biotin-peroxidase complex solution (Vectastain ABC kit, Vector Laboratories Inc., USA) for 1 h. The specific staining was visualized using 3,3′-diaminobenzidine (DAB Safe, Saveen Werner, Sweden) and 0.01 % H2 O2 . .. For preservation and visualization, sections were mounted on chromatin-gelatin coated glass slides, dried, dehydrated in increasing alcohol solutions, cleared in xylene and coverslipped using DPX (Sigma-Aldrich, Sweden).

    Article Title: Fibroblast Heterogeneity
    Article Snippet: An isotype control antibody mouse IgG1 (Caltag, Burlingame, CA) was included at 10 μg/ml. .. Sections were then incubated with biotinylated horse anti-mouse IgG (Vector Laboratories) and the avidin-biotin-peroxidase detection system was used (Elite ABC 6101; Vector Laboratories). .. Immunofluorescence was used to determine Thy 1 expression in the myometrial and endometrial fibroblast strains.

    Staining:

    Article Title: Novel Chlamydia muridarum T cell Antigens Induce Protective Immunity against Lung and Genital Tract Infection in Murine Models
    Article Snippet: All anti- Chlamydia recombinant protein polyclonal antibodies had titers ≥1: 500,000 dilution as determined by ELISA. .. Biotinylated horse anti-mouse IgG (1: 2000) (Vector Laboratories) was added and then the cells were incubated again for 1 h. Finally, the cells were incubated for 45 min with ABC Reagent (Vector Laboratories) and incubated with peroxidase substrate solution (DAB substrate kit SK-4100; Vector Laboratories) until the desired stain intensity developed. ..

    Article Title: Dysregulated expression of cell surface glycoprotein CDCP1 in prostate cancer
    Article Snippet: The next day slides were blocked with 3% H2 O2 and avidin/biotin blocking kit (Vector Lab). .. Secondary staining was performed with biotinylated horse anti-mouse antibodies (50 μg/ml, Vector Lab) and visualized with Vectastain ABC kit (Vector Lab) and 3, 3′-diaminobenzidine (DAB)- H2 O2 Substrate (BD Biosciences). ..

    Article Title: A novel multiplex assay for simultaneous quantification of total and S129 phosphorylated human alpha-synuclein
    Article Snippet: .. The next day sections were rinsed with TBS-T and incubated with horse anti mouse biotinylated secondary antibody (1:200, Vector Laboratories Inc.) in 1 % BSA in TBS-T for 1 h. Sections were again washed with TBS-T and incubated with an avidin-biotin-peroxidase complex solution (Vectastain ABC kit, Vector Laboratories Inc., USA) for 1 h. The specific staining was visualized using 3,3′-diaminobenzidine (DAB Safe, Saveen Werner, Sweden) and 0.01 % H2 O2 . .. For preservation and visualization, sections were mounted on chromatin-gelatin coated glass slides, dried, dehydrated in increasing alcohol solutions, cleared in xylene and coverslipped using DPX (Sigma-Aldrich, Sweden).

    Binding Assay:

    Article Title: Inflammation and Extracellular Matrix Degradation Mediated by Activated Transcription Factors Nuclear Factor-?B and Activator Protein-1 in Inflammatory Acne Lesions in Vivo
    Article Snippet: For NF-κB p65, sections were preincubated with normal goat serum then stained with a mouse monoclonal p65 antibody (F-6, 1:200; Santa Cruz Biotechnology). .. Binding of the antibodies was visualized by biotinylated goat anti-rabbit (Vector Laboratories, Burlingame, CA) for p50, and by biotinylated horse anti-mouse (Vector Laboratories) in combination with Texas Red (Calbiochem, San Diego, CA) for p65. .. Isotype control immunoglobulin that was substituted for each primary antibody yielded no detectable staining.

    Immunofluorescence:

    Article Title: Shift of C3 deposition from localization in the glomerulus into the tubulo-interstitial compartment in the absence of secreted IgM in immune complex glomerulonephritis
    Article Snippet: .. To assess renal IgG, IgM and C3 IC deposits by immunofluorescence and confocal laser scanning microscopy, FITC-conjugated rat anti-mouse IgM (5 μg/ml; Pharmingen), purified goat anti-mouse C3 (4 μg/ml; Cappel, Aurora, CA, USA) and biotin-conjugated horse anti-mouse IgG (H + L) antibody (1 μg/ml; Vector, Burlingame, CA, USA) were diluted in TBS/1% BSA and applied overnight at 4°C. .. Detection was performed with tetra-methylrhodamine isothiocyanate (TRITC)-conjugated rabbit anti-goat IgG F(ab′)2 (1·4 μg/ml; Jackson Immunoresearch, West Grove, PA, USA) and streptavidin Cy5-conjugate (2 μg/ml; Jackson Immunoresearch).

    Confocal Laser Scanning Microscopy:

    Article Title: Shift of C3 deposition from localization in the glomerulus into the tubulo-interstitial compartment in the absence of secreted IgM in immune complex glomerulonephritis
    Article Snippet: .. To assess renal IgG, IgM and C3 IC deposits by immunofluorescence and confocal laser scanning microscopy, FITC-conjugated rat anti-mouse IgM (5 μg/ml; Pharmingen), purified goat anti-mouse C3 (4 μg/ml; Cappel, Aurora, CA, USA) and biotin-conjugated horse anti-mouse IgG (H + L) antibody (1 μg/ml; Vector, Burlingame, CA, USA) were diluted in TBS/1% BSA and applied overnight at 4°C. .. Detection was performed with tetra-methylrhodamine isothiocyanate (TRITC)-conjugated rabbit anti-goat IgG F(ab′)2 (1·4 μg/ml; Jackson Immunoresearch, West Grove, PA, USA) and streptavidin Cy5-conjugate (2 μg/ml; Jackson Immunoresearch).

    Purification:

    Article Title: Shift of C3 deposition from localization in the glomerulus into the tubulo-interstitial compartment in the absence of secreted IgM in immune complex glomerulonephritis
    Article Snippet: .. To assess renal IgG, IgM and C3 IC deposits by immunofluorescence and confocal laser scanning microscopy, FITC-conjugated rat anti-mouse IgM (5 μg/ml; Pharmingen), purified goat anti-mouse C3 (4 μg/ml; Cappel, Aurora, CA, USA) and biotin-conjugated horse anti-mouse IgG (H + L) antibody (1 μg/ml; Vector, Burlingame, CA, USA) were diluted in TBS/1% BSA and applied overnight at 4°C. .. Detection was performed with tetra-methylrhodamine isothiocyanate (TRITC)-conjugated rabbit anti-goat IgG F(ab′)2 (1·4 μg/ml; Jackson Immunoresearch, West Grove, PA, USA) and streptavidin Cy5-conjugate (2 μg/ml; Jackson Immunoresearch).

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    • biotinylated horse anti mouse ig secondary ab  (Vector Laboratories)
    99
    Vector Laboratories biotinylated horse anti mouse ig secondary ab
    Differences in serum gp70 expression and glomerular pathology induced after injection of purified 12H5.1 IgG3 in NZW, (BALB/c × MRL/+)F 1 , and B6 mice. (a) Results of Western blotting assays showing the expression of serum gp70 in three different strains of mice. Sera were diluted 1:20 into SDS sample buffer without a reducing reagent and boiled for 5 min; 10 μl of each boiled mixture was loaded into a well of 7.5% polyacrylamide gel. Plasma from a 4 month-old female MRL/ lpr ), NZW mice expressed a high level of serum gp85 (gp70 plus p15E), gp70, and a degradation product gp45 (arrowheads), while their expression in B6 mice was low. (BALB/c × MRL/+)F 1 mice (F 1 ) expressed an intermediate level of serum gp70. Mr, <t>biotinylated</t> markers, with positions indicated in kilodaltons at the left. (b to d) Representative photomicrographs taken from kidney sections of NZW (b), (BALB/c × MRL/+)F 1 (c), and B6 (d) mice injected with purified anti-gp70 IgG3, 12H5.1; hematoxylin and eosin staining, ×300. Note apparent thickening of the capillary walls (arrowheads) and inflammatory cell infiltration (arrow) in panel b and marked increase in glomerular cellularity and evident neutrophilic infiltration in panel c.
    Biotinylated Horse Anti Mouse Ig Secondary Ab, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated horse anti mouse ig secondary ab/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated horse anti mouse ig secondary ab - by Bioz Stars, 2021-03
    99/100 stars
    		  Buy from Supplier
    biotinylated universal secondary antibody  (Vector Laboratories)
    97
    Vector Laboratories biotinylated universal secondary antibody
    Representative histological images of the insulin- and glucagon-immunoreactive cells in the pancreas, taken from NFD or HFD supplied mice. ( a ) Vehicle (distilled water) 10 mL/kg orally administered mice with NFD supply (Intact control); ( b ) Vehicle 10 mL/kg orally administered mice with HFD supply (HFD control); ( c ) 250 mg/kg of metformin oral administered mice with HFD supply (Metformin); ( d ) WL 200 mg/kg orally administered mice with HFD supply (WL200); ( e ) WL 100 mg/kg orally administered mice with HFD supply (WL100); ( f ) WL 50 mg/kg orally administered mice with HFD supply (WL50). NFD = Normal pellet diet; HFD = 45% Kcal high-fat diet; WL = Wasabi Leaf/Folium, Wasabia japonica (Miq.) Matsum extracts; IS = Pancreatic islet. All immunostained by <t>avidin-biotin-peroxidase</t> complex. Scale bars = 80 µm.
    Biotinylated Universal Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated universal secondary antibody/product/Vector Laboratories
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated universal secondary antibody - by Bioz Stars, 2021-03
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    Differences in serum gp70 expression and glomerular pathology induced after injection of purified 12H5.1 IgG3 in NZW, (BALB/c × MRL/+)F 1 , and B6 mice. (a) Results of Western blotting assays showing the expression of serum gp70 in three different strains of mice. Sera were diluted 1:20 into SDS sample buffer without a reducing reagent and boiled for 5 min; 10 μl of each boiled mixture was loaded into a well of 7.5% polyacrylamide gel. Plasma from a 4 month-old female MRL/ lpr ), NZW mice expressed a high level of serum gp85 (gp70 plus p15E), gp70, and a degradation product gp45 (arrowheads), while their expression in B6 mice was low. (BALB/c × MRL/+)F 1 mice (F 1 ) expressed an intermediate level of serum gp70. Mr, biotinylated markers, with positions indicated in kilodaltons at the left. (b to d) Representative photomicrographs taken from kidney sections of NZW (b), (BALB/c × MRL/+)F 1 (c), and B6 (d) mice injected with purified anti-gp70 IgG3, 12H5.1; hematoxylin and eosin staining, ×300. Note apparent thickening of the capillary walls (arrowheads) and inflammatory cell infiltration (arrow) in panel b and marked increase in glomerular cellularity and evident neutrophilic infiltration in panel c.

    Journal: Journal of Virology

    Article Title: Establishment of Monoclonal Anti-Retroviral gp70 Autoantibodies from MRL/lpr Lupus Mice and Induction of Glomerular gp70 Deposition and Pathology by Transfer into Non-Autoimmune Mice

    doi:

    Figure Lengend Snippet: Differences in serum gp70 expression and glomerular pathology induced after injection of purified 12H5.1 IgG3 in NZW, (BALB/c × MRL/+)F 1 , and B6 mice. (a) Results of Western blotting assays showing the expression of serum gp70 in three different strains of mice. Sera were diluted 1:20 into SDS sample buffer without a reducing reagent and boiled for 5 min; 10 μl of each boiled mixture was loaded into a well of 7.5% polyacrylamide gel. Plasma from a 4 month-old female MRL/ lpr ), NZW mice expressed a high level of serum gp85 (gp70 plus p15E), gp70, and a degradation product gp45 (arrowheads), while their expression in B6 mice was low. (BALB/c × MRL/+)F 1 mice (F 1 ) expressed an intermediate level of serum gp70. Mr, biotinylated markers, with positions indicated in kilodaltons at the left. (b to d) Representative photomicrographs taken from kidney sections of NZW (b), (BALB/c × MRL/+)F 1 (c), and B6 (d) mice injected with purified anti-gp70 IgG3, 12H5.1; hematoxylin and eosin staining, ×300. Note apparent thickening of the capillary walls (arrowheads) and inflammatory cell infiltration (arrow) in panel b and marked increase in glomerular cellularity and evident neutrophilic infiltration in panel c.

    Article Snippet: Incubation with MAb and detection of bound Ab by using biotinylated horse anti-mouse Ig secondary Ab and avidin-biotinylated peroxidase complex (Vector Laboratories, Burlingame, CA) has been described elsewhere ( , ).

    Techniques: Expressing, Injection, Purification, Mouse Assay, Western Blot, Staining

    Representative kidney pathology of hybridoma-transplanted mice. (a) (BALB/c × MRL/+)F 1 mouse transplanted with 8.653 fusion partner cells. PAS staining, ×70. (b) (BALB/c × MRL/+)F 1 mouse transplanted with hybridoma cells 12H5.1. PAS staining, ×70. Note the extreme expansion of the glomeruli compared to those in panel a, which shows normal glomeruli at the same magnification. Sizes of tubules and of the nuclei of tubular epithelial cells are not different in panels a and b, but glomeruli are markedly enlarged in panel b. Granular deposition of fibrin in the affected glomeruli was also shown when phosphotungstenic acid-hematoxylin staining was applied (not shown). (c) Immunofluorescence staining with FITC-conjugated anti-mouse IgG of a fresh-frozen section taken from a representative (BALB/c × MRL/+)F 1 mouse transplanted with hybridoma cells 12H5.1. Use of FITC-conjugated anti-mouse C3 resulted in a similar pattern of staining. (d) Electron micrograph showing an affected glomerulus of a representative (BALB/c × MRL/+)F 1 ) was biotinylated to detect the presence of xenotropic viral env gene products. (g) (BALB/c × MRL/+)F 1 mouse transplanted with hybridoma cells 37C4.1 showing typical wire loop lesions. PAS staining, ×175. (h) Electron micrograph of the kidney from a (BALB/c × MRL/+)F 1 mouse transplanted with hybridoma cells 37C4.1. Bar = 2 μm. Note the dense subendothelial deposits consistent with light microscopic wire loops along the basement membrane. (i) SCID mouse transplanted with hybridoma cells 37C4.1. PAS staining, ×140. (j) Dense linear deposition of mouse C3 in a representative glomerulus from a SCID mouse transplanted with hybridoma 37C4.1. Similar deposition of mouse IgG and xenotropic viral gp70 in the affected glomeruli was also demonstrated in fresh-frozen sections of the transplanted SCID mice. (k and l) (BALB/c × MRL/+)F 1 mice transplanted with one clone of hybridomas 51D1.1 and 59C4.1, respectively. PAS staining, ×140. Note PAS-positive deposition and expansion of the mesangial areas in panel k and cell proliferation (arrowheads) and occlusive changes (arrow) of capillaries in panel l. Lesions similar to those in panel l were observed in the mice transplanted with hybridoma 60A5.1 (not shown).

    Journal: Journal of Virology

    Article Title: Establishment of Monoclonal Anti-Retroviral gp70 Autoantibodies from MRL/lpr Lupus Mice and Induction of Glomerular gp70 Deposition and Pathology by Transfer into Non-Autoimmune Mice

    doi:

    Figure Lengend Snippet: Representative kidney pathology of hybridoma-transplanted mice. (a) (BALB/c × MRL/+)F 1 mouse transplanted with 8.653 fusion partner cells. PAS staining, ×70. (b) (BALB/c × MRL/+)F 1 mouse transplanted with hybridoma cells 12H5.1. PAS staining, ×70. Note the extreme expansion of the glomeruli compared to those in panel a, which shows normal glomeruli at the same magnification. Sizes of tubules and of the nuclei of tubular epithelial cells are not different in panels a and b, but glomeruli are markedly enlarged in panel b. Granular deposition of fibrin in the affected glomeruli was also shown when phosphotungstenic acid-hematoxylin staining was applied (not shown). (c) Immunofluorescence staining with FITC-conjugated anti-mouse IgG of a fresh-frozen section taken from a representative (BALB/c × MRL/+)F 1 mouse transplanted with hybridoma cells 12H5.1. Use of FITC-conjugated anti-mouse C3 resulted in a similar pattern of staining. (d) Electron micrograph showing an affected glomerulus of a representative (BALB/c × MRL/+)F 1 ) was biotinylated to detect the presence of xenotropic viral env gene products. (g) (BALB/c × MRL/+)F 1 mouse transplanted with hybridoma cells 37C4.1 showing typical wire loop lesions. PAS staining, ×175. (h) Electron micrograph of the kidney from a (BALB/c × MRL/+)F 1 mouse transplanted with hybridoma cells 37C4.1. Bar = 2 μm. Note the dense subendothelial deposits consistent with light microscopic wire loops along the basement membrane. (i) SCID mouse transplanted with hybridoma cells 37C4.1. PAS staining, ×140. (j) Dense linear deposition of mouse C3 in a representative glomerulus from a SCID mouse transplanted with hybridoma 37C4.1. Similar deposition of mouse IgG and xenotropic viral gp70 in the affected glomeruli was also demonstrated in fresh-frozen sections of the transplanted SCID mice. (k and l) (BALB/c × MRL/+)F 1 mice transplanted with one clone of hybridomas 51D1.1 and 59C4.1, respectively. PAS staining, ×140. Note PAS-positive deposition and expansion of the mesangial areas in panel k and cell proliferation (arrowheads) and occlusive changes (arrow) of capillaries in panel l. Lesions similar to those in panel l were observed in the mice transplanted with hybridoma 60A5.1 (not shown).

    Article Snippet: Incubation with MAb and detection of bound Ab by using biotinylated horse anti-mouse Ig secondary Ab and avidin-biotinylated peroxidase complex (Vector Laboratories, Burlingame, CA) has been described elsewhere ( , ).

    Techniques: Mouse Assay, Staining, Immunofluorescence

    Representative histological images of the insulin- and glucagon-immunoreactive cells in the pancreas, taken from NFD or HFD supplied mice. ( a ) Vehicle (distilled water) 10 mL/kg orally administered mice with NFD supply (Intact control); ( b ) Vehicle 10 mL/kg orally administered mice with HFD supply (HFD control); ( c ) 250 mg/kg of metformin oral administered mice with HFD supply (Metformin); ( d ) WL 200 mg/kg orally administered mice with HFD supply (WL200); ( e ) WL 100 mg/kg orally administered mice with HFD supply (WL100); ( f ) WL 50 mg/kg orally administered mice with HFD supply (WL50). NFD = Normal pellet diet; HFD = 45% Kcal high-fat diet; WL = Wasabi Leaf/Folium, Wasabia japonica (Miq.) Matsum extracts; IS = Pancreatic islet. All immunostained by avidin-biotin-peroxidase complex. Scale bars = 80 µm.

    Journal: Nutrients

    Article Title: Anti-Diabetic Obesity Effects of Wasabia Japonica Matsum Leaf Extract on 45% Kcal High-Fat Diet-Fed Mice

    doi: 10.3390/nu12092837

    Figure Lengend Snippet: Representative histological images of the insulin- and glucagon-immunoreactive cells in the pancreas, taken from NFD or HFD supplied mice. ( a ) Vehicle (distilled water) 10 mL/kg orally administered mice with NFD supply (Intact control); ( b ) Vehicle 10 mL/kg orally administered mice with HFD supply (HFD control); ( c ) 250 mg/kg of metformin oral administered mice with HFD supply (Metformin); ( d ) WL 200 mg/kg orally administered mice with HFD supply (WL200); ( e ) WL 100 mg/kg orally administered mice with HFD supply (WL100); ( f ) WL 50 mg/kg orally administered mice with HFD supply (WL50). NFD = Normal pellet diet; HFD = 45% Kcal high-fat diet; WL = Wasabi Leaf/Folium, Wasabia japonica (Miq.) Matsum extracts; IS = Pancreatic islet. All immunostained by avidin-biotin-peroxidase complex. Scale bars = 80 µm.

    Article Snippet: This was followed by incubation with ABC reagents (Vectastain Elite ABC Kit, Vector Lab., Burlingame, CA, USA) and biotinylated universal secondary antibody (Vector Lab., Burlingame, CA, USA) in a humidity chamber for 1 h at room temperature.

    Techniques: Mouse Assay, Avidin-Biotin Assay

    Representative histological images of the insulin-immunoreactive cells in the pancreas, taken from intact normoglycemic or db/db mice. ( A ) Intact control mouse; ( B ) db control mouse; ( C ) metformin 250 mg/kg treated db mouse; ( D ) GT 400 mg/kg treated db mouse; ( E ) fGT 400 mg/kg treated db mouse; ( F ) fGT 200 mg/kg treated db mouse; ( G ) fGT 100 mg/kg treated db mouse. GT, Green tea aqueous lyophilized extracts; fGT, Aquilariae Lignum-fermented green tea aqueous lyophilized extracts; IS, pancreatic islet; PD, pancreatic secretory duct. All immunostained by avidin-biotin-peroxidase complex. Scale bars = 80 µm.

    Journal: Nutrients

    Article Title: Fermentation with Aquilariae Lignum Enhances the Anti-Diabetic Activity of Green Tea in Type II Diabetic db/db Mouse

    doi: 10.3390/nu6093536

    Figure Lengend Snippet: Representative histological images of the insulin-immunoreactive cells in the pancreas, taken from intact normoglycemic or db/db mice. ( A ) Intact control mouse; ( B ) db control mouse; ( C ) metformin 250 mg/kg treated db mouse; ( D ) GT 400 mg/kg treated db mouse; ( E ) fGT 400 mg/kg treated db mouse; ( F ) fGT 200 mg/kg treated db mouse; ( G ) fGT 100 mg/kg treated db mouse. GT, Green tea aqueous lyophilized extracts; fGT, Aquilariae Lignum-fermented green tea aqueous lyophilized extracts; IS, pancreatic islet; PD, pancreatic secretory duct. All immunostained by avidin-biotin-peroxidase complex. Scale bars = 80 µm.

    Article Snippet: Next day, the sections were incubated with biotinylated universal secondary antibody (Vector Lab., dilution 1:50) for 1 h at room temperature, and then avidin-biotin-peroxidase reagents (Vectastain Elite ABC Kit, Vector Lab., dilution 1:50) according to ABC methods [ ].

    Techniques: Mouse Assay, Avidin-Biotin Assay

    Representative histological images of the glucagon-immunoreactive cells in the pancreas, taken from intact normoglycemic or db/db mice. ( A ) Intact control mouse; ( B ) db control mouse; ( C ) metformin 250 mg/kg treated db mouse; ( D ) GT 400 mg/kg treated db mouse; ( E ) fGT 400 mg/kg treated db mouse; ( F ) fGT 200 mg/kg treated db mouse; ( G ) fGT 100 mg/kg treated db mouse. GT, Green tea aqueous lyophilized extracts; fGT, Aquilariae Lignum-fermented green tea aqueous lyophilized extracts; IS, pancreatic islet; PD, pancreatic secretory duct. All immunostained by avidin-biotin-peroxidase complex. Scale bars = 80 µm.

    Journal: Nutrients

    Article Title: Fermentation with Aquilariae Lignum Enhances the Anti-Diabetic Activity of Green Tea in Type II Diabetic db/db Mouse

    doi: 10.3390/nu6093536

    Figure Lengend Snippet: Representative histological images of the glucagon-immunoreactive cells in the pancreas, taken from intact normoglycemic or db/db mice. ( A ) Intact control mouse; ( B ) db control mouse; ( C ) metformin 250 mg/kg treated db mouse; ( D ) GT 400 mg/kg treated db mouse; ( E ) fGT 400 mg/kg treated db mouse; ( F ) fGT 200 mg/kg treated db mouse; ( G ) fGT 100 mg/kg treated db mouse. GT, Green tea aqueous lyophilized extracts; fGT, Aquilariae Lignum-fermented green tea aqueous lyophilized extracts; IS, pancreatic islet; PD, pancreatic secretory duct. All immunostained by avidin-biotin-peroxidase complex. Scale bars = 80 µm.

    Article Snippet: Next day, the sections were incubated with biotinylated universal secondary antibody (Vector Lab., dilution 1:50) for 1 h at room temperature, and then avidin-biotin-peroxidase reagents (Vectastain Elite ABC Kit, Vector Lab., dilution 1:50) according to ABC methods [ ].

    Techniques: Mouse Assay, Avidin-Biotin Assay

    Examples of anterograde and retrograde tracer injection sites. Shown are a Pha-L site in POR, 40P at −8.1 mm relative to β (A), a biotinylated dextrane site in the LEA, 61B at −6.5 mm relative to β (B), a diamidino yellow

    Journal: Behavioural brain research

    Article Title: HIPPOCAMPAL AND SUBICULAR EFFERENTS AND AFFERENTS OF THE PERIRHINAL, POSTRHINAL, AND ENTORHINAL CORTICES OF THE RAT

    doi: 10.1016/j.bbr.2013.07.005

    Figure Lengend Snippet: Examples of anterograde and retrograde tracer injection sites. Shown are a Pha-L site in POR, 40P at −8.1 mm relative to β (A), a biotinylated dextrane site in the LEA, 61B at −6.5 mm relative to β (B), a diamidino yellow

    Article Snippet: Following two 10 minute washes in 2% NGS in KPBS, sections were incubated in the biotinylated secondary antibody solution of goat anti-rabbit IgG (1:277 dilution; Vector Laboratories, Burlingame, CA), 0.3% TX, and 2% NGS in KPBS for 1 hr.

    Techniques: Injection