biotinylated horse anti mouse igg  (Vector Laboratories)


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    Name:
    Biotinylated Horse Anti Mouse IgG Antibody
    Description:
    Biotinylated Horse Anti Mouse IgG Antibodyd is prepared using proprietary immunization schedules that produce high affinity antibodies The antibodies are then purified by affinity chromatography and cross reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining ELISAs and blots Biotinylated Horse Anti Mouse IgG H L is supplied in solution With some exceptions the recommended dilution for most applications is 1 200 H L indicates the antibody recognizes both heavy and light chains This antibody is included in the VECTASTAIN ABC kits
    Catalog Number:
    ba-2000
    Price:
    None
    Category:
    Antibodies
    Reactivity:
    Mouse
    Size:
    1 5 mg
    Host:
    Horse
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    Structured Review

    Vector Laboratories biotinylated horse anti mouse igg
    Biotinylated Horse Anti Mouse IgG Antibody
    Biotinylated Horse Anti Mouse IgG Antibodyd is prepared using proprietary immunization schedules that produce high affinity antibodies The antibodies are then purified by affinity chromatography and cross reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining ELISAs and blots Biotinylated Horse Anti Mouse IgG H L is supplied in solution With some exceptions the recommended dilution for most applications is 1 200 H L indicates the antibody recognizes both heavy and light chains This antibody is included in the VECTASTAIN ABC kits
    https://www.bioz.com/result/biotinylated horse anti mouse igg/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated horse anti mouse igg - by Bioz Stars, 2021-03
    86/100 stars

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    Related Articles

    Incubation:

    Article Title: ADAM12 Is Selectively Overexpressed in Human Glioblastomas and Is Associated with Glioblastoma Cell Proliferation and Shedding of Heparin-Binding Epidermal Growth Factor
    Article Snippet: After blocking nonspecific binding with 10% horse serum for ADAM12m staining or 10% goat serum for Ki-67 staining, they were incubated with mouse monoclonal antibodies against ADAM12m (283-6H3, 5 μg/ml) or Ki-67 (MIB1, 1/50 dilution; DakoCytomation Norden A/S). .. Subsequently, the specimens were incubated with biotinylated horse antibodies against mouse IgG (1/200 dilution; Vector Laboratories, Inc., Burlingame, CA) followed by the ABC method for ADAM12m or with goat antibodies against mouse IgG conjugated to horseradish peroxidase-labeled dextran polymer (no dilution, EnVision+ Peroxidase Mouse; DakoCytomation, California Inc., Carpinteria, CA) for Ki-67. ..

    Article Title: Establishment of Monoclonal Anti-Retroviral gp70 Autoantibodies from MRL/lpr Lupus Mice and Induction of Glomerular gp70 Deposition and Pathology by Transfer into Non-Autoimmune Mice
    Article Snippet: Proteins separated through 7.5% polyacrylamide gels were transferred onto polyvinylidene difluoride membranes (Immobilon; Millipore Corporation, Bedford, Mass.) as described previously ( , ), and the blotted membrane was blocked with 10% skim milk. .. Incubation with MAb and detection of bound Ab by using biotinylated horse anti-mouse Ig secondary Ab and avidin-biotinylated peroxidase complex (Vector Laboratories, Burlingame, CA) has been described elsewhere ( , ). .. For the detection of serum gp70, sera from NZW, (BALB/c × MRL/+)F1 , and B6 mice were mixed at 1:20 with the SDS sample buffer containing no reducing agent, and serum proteins were separated through 7.5% polyacrylamide gels and blotted as described above.

    Article Title: Novel Chlamydia muridarum T cell Antigens Induce Protective Immunity against Lung and Genital Tract Infection in Murine Models
    Article Snippet: All anti- Chlamydia recombinant protein polyclonal antibodies had titers ≥1: 500,000 dilution as determined by ELISA. .. Biotinylated horse anti-mouse IgG (1: 2000) (Vector Laboratories) was added and then the cells were incubated again for 1 h. Finally, the cells were incubated for 45 min with ABC Reagent (Vector Laboratories) and incubated with peroxidase substrate solution (DAB substrate kit SK-4100; Vector Laboratories) until the desired stain intensity developed. ..

    Article Title: A novel multiplex assay for simultaneous quantification of total and S129 phosphorylated human alpha-synuclein
    Article Snippet: .. The next day sections were rinsed with TBS-T and incubated with horse anti mouse biotinylated secondary antibody (1:200, Vector Laboratories Inc.) in 1 % BSA in TBS-T for 1 h. Sections were again washed with TBS-T and incubated with an avidin-biotin-peroxidase complex solution (Vectastain ABC kit, Vector Laboratories Inc., USA) for 1 h. The specific staining was visualized using 3,3′-diaminobenzidine (DAB Safe, Saveen Werner, Sweden) and 0.01 % H2 O2 . .. For preservation and visualization, sections were mounted on chromatin-gelatin coated glass slides, dried, dehydrated in increasing alcohol solutions, cleared in xylene and coverslipped using DPX (Sigma-Aldrich, Sweden).

    Article Title: Fibroblast Heterogeneity
    Article Snippet: An isotype control antibody mouse IgG1 (Caltag, Burlingame, CA) was included at 10 μg/ml. .. Sections were then incubated with biotinylated horse anti-mouse IgG (Vector Laboratories) and the avidin-biotin-peroxidase detection system was used (Elite ABC 6101; Vector Laboratories). .. Immunofluorescence was used to determine Thy 1 expression in the myometrial and endometrial fibroblast strains.

    Avidin-Biotin Assay:

    Article Title: Establishment of Monoclonal Anti-Retroviral gp70 Autoantibodies from MRL/lpr Lupus Mice and Induction of Glomerular gp70 Deposition and Pathology by Transfer into Non-Autoimmune Mice
    Article Snippet: Proteins separated through 7.5% polyacrylamide gels were transferred onto polyvinylidene difluoride membranes (Immobilon; Millipore Corporation, Bedford, Mass.) as described previously ( , ), and the blotted membrane was blocked with 10% skim milk. .. Incubation with MAb and detection of bound Ab by using biotinylated horse anti-mouse Ig secondary Ab and avidin-biotinylated peroxidase complex (Vector Laboratories, Burlingame, CA) has been described elsewhere ( , ). .. For the detection of serum gp70, sera from NZW, (BALB/c × MRL/+)F1 , and B6 mice were mixed at 1:20 with the SDS sample buffer containing no reducing agent, and serum proteins were separated through 7.5% polyacrylamide gels and blotted as described above.

    Article Title: A novel multiplex assay for simultaneous quantification of total and S129 phosphorylated human alpha-synuclein
    Article Snippet: .. The next day sections were rinsed with TBS-T and incubated with horse anti mouse biotinylated secondary antibody (1:200, Vector Laboratories Inc.) in 1 % BSA in TBS-T for 1 h. Sections were again washed with TBS-T and incubated with an avidin-biotin-peroxidase complex solution (Vectastain ABC kit, Vector Laboratories Inc., USA) for 1 h. The specific staining was visualized using 3,3′-diaminobenzidine (DAB Safe, Saveen Werner, Sweden) and 0.01 % H2 O2 . .. For preservation and visualization, sections were mounted on chromatin-gelatin coated glass slides, dried, dehydrated in increasing alcohol solutions, cleared in xylene and coverslipped using DPX (Sigma-Aldrich, Sweden).

    Article Title: Fibroblast Heterogeneity
    Article Snippet: An isotype control antibody mouse IgG1 (Caltag, Burlingame, CA) was included at 10 μg/ml. .. Sections were then incubated with biotinylated horse anti-mouse IgG (Vector Laboratories) and the avidin-biotin-peroxidase detection system was used (Elite ABC 6101; Vector Laboratories). .. Immunofluorescence was used to determine Thy 1 expression in the myometrial and endometrial fibroblast strains.

    Staining:

    Article Title: Novel Chlamydia muridarum T cell Antigens Induce Protective Immunity against Lung and Genital Tract Infection in Murine Models
    Article Snippet: All anti- Chlamydia recombinant protein polyclonal antibodies had titers ≥1: 500,000 dilution as determined by ELISA. .. Biotinylated horse anti-mouse IgG (1: 2000) (Vector Laboratories) was added and then the cells were incubated again for 1 h. Finally, the cells were incubated for 45 min with ABC Reagent (Vector Laboratories) and incubated with peroxidase substrate solution (DAB substrate kit SK-4100; Vector Laboratories) until the desired stain intensity developed. ..

    Article Title: Dysregulated expression of cell surface glycoprotein CDCP1 in prostate cancer
    Article Snippet: The next day slides were blocked with 3% H2 O2 and avidin/biotin blocking kit (Vector Lab). .. Secondary staining was performed with biotinylated horse anti-mouse antibodies (50 μg/ml, Vector Lab) and visualized with Vectastain ABC kit (Vector Lab) and 3, 3′-diaminobenzidine (DAB)- H2 O2 Substrate (BD Biosciences). ..

    Article Title: A novel multiplex assay for simultaneous quantification of total and S129 phosphorylated human alpha-synuclein
    Article Snippet: .. The next day sections were rinsed with TBS-T and incubated with horse anti mouse biotinylated secondary antibody (1:200, Vector Laboratories Inc.) in 1 % BSA in TBS-T for 1 h. Sections were again washed with TBS-T and incubated with an avidin-biotin-peroxidase complex solution (Vectastain ABC kit, Vector Laboratories Inc., USA) for 1 h. The specific staining was visualized using 3,3′-diaminobenzidine (DAB Safe, Saveen Werner, Sweden) and 0.01 % H2 O2 . .. For preservation and visualization, sections were mounted on chromatin-gelatin coated glass slides, dried, dehydrated in increasing alcohol solutions, cleared in xylene and coverslipped using DPX (Sigma-Aldrich, Sweden).

    Binding Assay:

    Article Title: Inflammation and Extracellular Matrix Degradation Mediated by Activated Transcription Factors Nuclear Factor-?B and Activator Protein-1 in Inflammatory Acne Lesions in Vivo
    Article Snippet: For NF-κB p65, sections were preincubated with normal goat serum then stained with a mouse monoclonal p65 antibody (F-6, 1:200; Santa Cruz Biotechnology). .. Binding of the antibodies was visualized by biotinylated goat anti-rabbit (Vector Laboratories, Burlingame, CA) for p50, and by biotinylated horse anti-mouse (Vector Laboratories) in combination with Texas Red (Calbiochem, San Diego, CA) for p65. .. Isotype control immunoglobulin that was substituted for each primary antibody yielded no detectable staining.

    Immunofluorescence:

    Article Title: Shift of C3 deposition from localization in the glomerulus into the tubulo-interstitial compartment in the absence of secreted IgM in immune complex glomerulonephritis
    Article Snippet: .. To assess renal IgG, IgM and C3 IC deposits by immunofluorescence and confocal laser scanning microscopy, FITC-conjugated rat anti-mouse IgM (5 μg/ml; Pharmingen), purified goat anti-mouse C3 (4 μg/ml; Cappel, Aurora, CA, USA) and biotin-conjugated horse anti-mouse IgG (H + L) antibody (1 μg/ml; Vector, Burlingame, CA, USA) were diluted in TBS/1% BSA and applied overnight at 4°C. .. Detection was performed with tetra-methylrhodamine isothiocyanate (TRITC)-conjugated rabbit anti-goat IgG F(ab′)2 (1·4 μg/ml; Jackson Immunoresearch, West Grove, PA, USA) and streptavidin Cy5-conjugate (2 μg/ml; Jackson Immunoresearch).

    Confocal Laser Scanning Microscopy:

    Article Title: Shift of C3 deposition from localization in the glomerulus into the tubulo-interstitial compartment in the absence of secreted IgM in immune complex glomerulonephritis
    Article Snippet: .. To assess renal IgG, IgM and C3 IC deposits by immunofluorescence and confocal laser scanning microscopy, FITC-conjugated rat anti-mouse IgM (5 μg/ml; Pharmingen), purified goat anti-mouse C3 (4 μg/ml; Cappel, Aurora, CA, USA) and biotin-conjugated horse anti-mouse IgG (H + L) antibody (1 μg/ml; Vector, Burlingame, CA, USA) were diluted in TBS/1% BSA and applied overnight at 4°C. .. Detection was performed with tetra-methylrhodamine isothiocyanate (TRITC)-conjugated rabbit anti-goat IgG F(ab′)2 (1·4 μg/ml; Jackson Immunoresearch, West Grove, PA, USA) and streptavidin Cy5-conjugate (2 μg/ml; Jackson Immunoresearch).

    Purification:

    Article Title: Shift of C3 deposition from localization in the glomerulus into the tubulo-interstitial compartment in the absence of secreted IgM in immune complex glomerulonephritis
    Article Snippet: .. To assess renal IgG, IgM and C3 IC deposits by immunofluorescence and confocal laser scanning microscopy, FITC-conjugated rat anti-mouse IgM (5 μg/ml; Pharmingen), purified goat anti-mouse C3 (4 μg/ml; Cappel, Aurora, CA, USA) and biotin-conjugated horse anti-mouse IgG (H + L) antibody (1 μg/ml; Vector, Burlingame, CA, USA) were diluted in TBS/1% BSA and applied overnight at 4°C. .. Detection was performed with tetra-methylrhodamine isothiocyanate (TRITC)-conjugated rabbit anti-goat IgG F(ab′)2 (1·4 μg/ml; Jackson Immunoresearch, West Grove, PA, USA) and streptavidin Cy5-conjugate (2 μg/ml; Jackson Immunoresearch).

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  • 99
    Vector Laboratories biotinylated antirat igg
    TECs exhibit different cellular distributions of endothelial molecules than normal MHECs. TECs or MHECs were plated at subculture 2 onto glass coverslips precoated with either OnFN or hFN, respectively. At 2 days postconfluence, the cells were washed with DPBS + and fixed in ice-cold methanol for 5 min at -20°C. Cell surface adhesion molecules (CD31, β-catenin, ICAM-2, and VCAM-1) were stained with specific antibodies as described in Materials and Methods section and detected with <t>antirat</t> or antirabbit <t>IgG</t> conjugated to Texas Red or FITC. Fluorescence images were captured on an Axiovert inverted microscope equipped for fluorescence using a cooled CCD camera and IP Laboratories software. All images displayed were captured with a x 40 objective. These images are representative of three separate cultures of both MHECs and TECs generated from different source tissues at different times. Images of MHECs are shown in the left hand panels (a,c,e,g) and of TECs in the right hand panels (b,d,f,g) with staining for CD31 (a,b), β-catenin (c,d), ICAM-2 (e,f), and VCAM-1 (g,h). Junctional staining of CD31 and β-catenin was discontinuous in TECs, indicating the presence of fewer cell-cell junctions. In addition, VCAM-1 did not localize to cell-cell junctions in TECs. In contrast, normal MHECs showed continuous junctional staining for CD31, β-catenin, and VCAM-1. There was little difference in distribution of ICAM-2 between MHECs and TECs.
    Biotinylated Antirat Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated antirat igg/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    99
    Vector Laboratories glun2b
    <t>GluN2B</t> expression is globally increased in CBA/J mice 4 months postnatally. ( A ) Bar charts represent mean GluN2B receptor optical densities in different brain regions 4 months postnatally in CBA/J mice compared with (CBA/CaOlaHsd) controls. Receptor density is significantly increased in the piriform cortex (PiC), somatosensory cortex (SC), posterior parietal cortex (PPC), visual cortex (VC), auditory cortex (AuC), dentate gyrus (DG), CA1, CA3, and CA4. Data are means ± SEM. * P
    Glun2b, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glun2b/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glun2b - by Bioz Stars, 2021-03
    99/100 stars
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    Image Search Results


    TECs exhibit different cellular distributions of endothelial molecules than normal MHECs. TECs or MHECs were plated at subculture 2 onto glass coverslips precoated with either OnFN or hFN, respectively. At 2 days postconfluence, the cells were washed with DPBS + and fixed in ice-cold methanol for 5 min at -20°C. Cell surface adhesion molecules (CD31, β-catenin, ICAM-2, and VCAM-1) were stained with specific antibodies as described in Materials and Methods section and detected with antirat or antirabbit IgG conjugated to Texas Red or FITC. Fluorescence images were captured on an Axiovert inverted microscope equipped for fluorescence using a cooled CCD camera and IP Laboratories software. All images displayed were captured with a x 40 objective. These images are representative of three separate cultures of both MHECs and TECs generated from different source tissues at different times. Images of MHECs are shown in the left hand panels (a,c,e,g) and of TECs in the right hand panels (b,d,f,g) with staining for CD31 (a,b), β-catenin (c,d), ICAM-2 (e,f), and VCAM-1 (g,h). Junctional staining of CD31 and β-catenin was discontinuous in TECs, indicating the presence of fewer cell-cell junctions. In addition, VCAM-1 did not localize to cell-cell junctions in TECs. In contrast, normal MHECs showed continuous junctional staining for CD31, β-catenin, and VCAM-1. There was little difference in distribution of ICAM-2 between MHECs and TECs.

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Murine Lewis Lung Carcinoma-Derived Endothelium Expresses Markers of Endothelial Activation and Requires Tumor-Specific Extracellular Matrix In Vitro 1

    doi:

    Figure Lengend Snippet: TECs exhibit different cellular distributions of endothelial molecules than normal MHECs. TECs or MHECs were plated at subculture 2 onto glass coverslips precoated with either OnFN or hFN, respectively. At 2 days postconfluence, the cells were washed with DPBS + and fixed in ice-cold methanol for 5 min at -20°C. Cell surface adhesion molecules (CD31, β-catenin, ICAM-2, and VCAM-1) were stained with specific antibodies as described in Materials and Methods section and detected with antirat or antirabbit IgG conjugated to Texas Red or FITC. Fluorescence images were captured on an Axiovert inverted microscope equipped for fluorescence using a cooled CCD camera and IP Laboratories software. All images displayed were captured with a x 40 objective. These images are representative of three separate cultures of both MHECs and TECs generated from different source tissues at different times. Images of MHECs are shown in the left hand panels (a,c,e,g) and of TECs in the right hand panels (b,d,f,g) with staining for CD31 (a,b), β-catenin (c,d), ICAM-2 (e,f), and VCAM-1 (g,h). Junctional staining of CD31 and β-catenin was discontinuous in TECs, indicating the presence of fewer cell-cell junctions. In addition, VCAM-1 did not localize to cell-cell junctions in TECs. In contrast, normal MHECs showed continuous junctional staining for CD31, β-catenin, and VCAM-1. There was little difference in distribution of ICAM-2 between MHECs and TECs.

    Article Snippet: Serial sections were stained with antibodies to CD31, CD102, CD144, CD106, Flk-1, and CD62E at 1 µg/ml, detected with biotinylated antirat IgG, and developed with the NovaRED substrate kit (Vector Laboratories).

    Techniques: Staining, Fluorescence, Inverted Microscopy, Software, Generated

    Comparison of untreated KAT-4 and Capan-2 carcinoma models. a Hematoxylin and Eosin and Sirius red staining in KAT-4 and Capan-2 carcinomas (bars = 100 μm). Trichrome staining (bars = 20 μm) and immunofluorescence staining with biotinylated 3G9 antibody (green) shows that the expression of integrin α V β 6 is located at the cell membrane in both KAT-4 and Capan-2 carcinomas (cell nuclei stained with DAPI, blue; bars = 50 μm). b Collagen content in untreated KAT-4 ( n = 4) and Capan-2 ( n = 5) carcinomas, represented by hydroxyproline mg/g wet weight. c Average growth of untreated KAT-4 ( n = 8) and Capan-2 tumors ( n = 7), represented in mm 3 measured externally (length x width x height)

    Journal: Cell Communication and Signaling : CCS

    Article Title: Inhibition of integrin αVβ6 changes fibril thickness of stromal collagen in experimental carcinomas

    doi: 10.1186/s12964-018-0249-7

    Figure Lengend Snippet: Comparison of untreated KAT-4 and Capan-2 carcinoma models. a Hematoxylin and Eosin and Sirius red staining in KAT-4 and Capan-2 carcinomas (bars = 100 μm). Trichrome staining (bars = 20 μm) and immunofluorescence staining with biotinylated 3G9 antibody (green) shows that the expression of integrin α V β 6 is located at the cell membrane in both KAT-4 and Capan-2 carcinomas (cell nuclei stained with DAPI, blue; bars = 50 μm). b Collagen content in untreated KAT-4 ( n = 4) and Capan-2 ( n = 5) carcinomas, represented by hydroxyproline mg/g wet weight. c Average growth of untreated KAT-4 ( n = 8) and Capan-2 tumors ( n = 7), represented in mm 3 measured externally (length x width x height)

    Article Snippet: Sections were blocked in 5% swine serum (Sigma) and 2% BSA (Sigma), incubated with biotinylated 3G9 antibody and then with Fluorescein Avidin D (Vector Labs).

    Techniques: Staining, Immunofluorescence, Expressing

    E06-scFv decreases early aortic valve stenosis, hepatic steatosis, and systemic inflammation a, b, Ldlr −/− (n=11) and Ldlr −/− / E06-scFv (n=10) mice were fed HCD for 15 months and prospectively examined at 3 time points for aortic valve hemodynamics. a , Mean pressure gradients across the aortic valve, determined by Doppler echocardiography. At 12 months there was a 49% lower mean gradient in the Ldlr −/− / E06-scFv mice (2.4±1.9mmHg vs. 4.8±2.4mmHg, mean±SD, p = 0.026, Ldlr −/− / E06-scFv (n=10) and Ldlr −/− (n=9). c, d , Calcification in aortic valve leaflets was determined by von Kossa staining of serial aortic valve sections and AUC compared. AV calcium was reduced in Ldlr −/− / E06-scFv mice by 41.5% (p=0.045, one-tailed-t-test, Ldlr −/− / E06-scFv (n=9) and Ldlr −/− (n=8)). e , Survival of mice used in AV hemodynamic study over 15 months. f , Hepatic cholesterol and triglyceride (TG) levels were reduced by 42% and 47% respectively in Ldlr −/− /E06-scFv mice, Ldlr −/− (n=10) and Ldlr −/− / E06-scFv (n=12) mice g , Livers of mice fed HCD for 16-wks were immunostained with biotinylated E06 IgM (brown) and compared to chow-fed C57BL/6 mice. Shown are representative photomicrographs, representative of 7 Ldlr −/− , 7 Ldlr −/− / E06-scFv and 3 WT (C57BL/6) mice. h , Plasma serum amyloid A (SAA) was decreased 32% in HC fed Ldlr −/− / E06-scFv mice ( Ldlr −/− (n=10) and Ldlr −/− / E06-scFv (n=12) mice).

    Journal: Nature

    Article Title: Oxidized Phospholipids are Proinflammatory and Proatherogenic in Hypercholesterolemic Mice

    doi: 10.1038/s41586-018-0198-8

    Figure Lengend Snippet: E06-scFv decreases early aortic valve stenosis, hepatic steatosis, and systemic inflammation a, b, Ldlr −/− (n=11) and Ldlr −/− / E06-scFv (n=10) mice were fed HCD for 15 months and prospectively examined at 3 time points for aortic valve hemodynamics. a , Mean pressure gradients across the aortic valve, determined by Doppler echocardiography. At 12 months there was a 49% lower mean gradient in the Ldlr −/− / E06-scFv mice (2.4±1.9mmHg vs. 4.8±2.4mmHg, mean±SD, p = 0.026, Ldlr −/− / E06-scFv (n=10) and Ldlr −/− (n=9). c, d , Calcification in aortic valve leaflets was determined by von Kossa staining of serial aortic valve sections and AUC compared. AV calcium was reduced in Ldlr −/− / E06-scFv mice by 41.5% (p=0.045, one-tailed-t-test, Ldlr −/− / E06-scFv (n=9) and Ldlr −/− (n=8)). e , Survival of mice used in AV hemodynamic study over 15 months. f , Hepatic cholesterol and triglyceride (TG) levels were reduced by 42% and 47% respectively in Ldlr −/− /E06-scFv mice, Ldlr −/− (n=10) and Ldlr −/− / E06-scFv (n=12) mice g , Livers of mice fed HCD for 16-wks were immunostained with biotinylated E06 IgM (brown) and compared to chow-fed C57BL/6 mice. Shown are representative photomicrographs, representative of 7 Ldlr −/− , 7 Ldlr −/− / E06-scFv and 3 WT (C57BL/6) mice. h , Plasma serum amyloid A (SAA) was decreased 32% in HC fed Ldlr −/− / E06-scFv mice ( Ldlr −/− (n=10) and Ldlr −/− / E06-scFv (n=12) mice).

    Article Snippet: Biotinylated antibodies (E06, anti-myc and anti-polyHis) were revealed with ABC-HRP VectaStain kit (Vector Laboratories, Burlingame, California) and/or NovaRed substrate (Vector Labs).

    Techniques: Mouse Assay, Staining, One-tailed Test

    Plasma E06-scFv binds to atherosclerotic lesions and apoptotic thymocytes and is present in aorta of Ldlr −/− /E06-scFv mice a , Staining of atherosclerotic lesions of WHHL rabbit aorta with E06-scFv plasma (left panel), and Ldlr −/− mice (right panel) (both at dilution of 1:20), visualized using biotinylated anti-Myc mAb and ABC-AP VectaStain kit. b , Deconvolution microscopy of E06-scFv plasma (1:20 dilution) binding to apoptotic but not normal cells. Blue, nuclei stained with Hoechst dye; Green, FITC-labeled anti-His tag mAb; Red, Annexin V-PE. c , Binding of E06-scFv plasma (1:20 dilution) to apoptotic thymocytes (7AAD+/Annexin V+) by FACS analysis. d , Expression of E06-scFv in aortic lesion of Ldlr −/− / E06-scFv but not Ldlr −/− mouse. Cross-sections at the AV were stained with biotinylated anti-Myc mAb to identify presence of E06-scFv in atherosclerotic lesion. Nuclei counterstained using Hematoxylin QS (Original ×200). Panels a-c are representative of similar studies with 5 other plasma samples. Panel d is representative of studies in 3 other aortic sections.

    Journal: Nature

    Article Title: Oxidized Phospholipids are Proinflammatory and Proatherogenic in Hypercholesterolemic Mice

    doi: 10.1038/s41586-018-0198-8

    Figure Lengend Snippet: Plasma E06-scFv binds to atherosclerotic lesions and apoptotic thymocytes and is present in aorta of Ldlr −/− /E06-scFv mice a , Staining of atherosclerotic lesions of WHHL rabbit aorta with E06-scFv plasma (left panel), and Ldlr −/− mice (right panel) (both at dilution of 1:20), visualized using biotinylated anti-Myc mAb and ABC-AP VectaStain kit. b , Deconvolution microscopy of E06-scFv plasma (1:20 dilution) binding to apoptotic but not normal cells. Blue, nuclei stained with Hoechst dye; Green, FITC-labeled anti-His tag mAb; Red, Annexin V-PE. c , Binding of E06-scFv plasma (1:20 dilution) to apoptotic thymocytes (7AAD+/Annexin V+) by FACS analysis. d , Expression of E06-scFv in aortic lesion of Ldlr −/− / E06-scFv but not Ldlr −/− mouse. Cross-sections at the AV were stained with biotinylated anti-Myc mAb to identify presence of E06-scFv in atherosclerotic lesion. Nuclei counterstained using Hematoxylin QS (Original ×200). Panels a-c are representative of similar studies with 5 other plasma samples. Panel d is representative of studies in 3 other aortic sections.

    Article Snippet: Biotinylated antibodies (E06, anti-myc and anti-polyHis) were revealed with ABC-HRP VectaStain kit (Vector Laboratories, Burlingame, California) and/or NovaRed substrate (Vector Labs).

    Techniques: Mouse Assay, Staining, Microscopy, Binding Assay, Labeling, FACS, Expressing

    GluN2B expression is globally increased in CBA/J mice 4 months postnatally. ( A ) Bar charts represent mean GluN2B receptor optical densities in different brain regions 4 months postnatally in CBA/J mice compared with (CBA/CaOlaHsd) controls. Receptor density is significantly increased in the piriform cortex (PiC), somatosensory cortex (SC), posterior parietal cortex (PPC), visual cortex (VC), auditory cortex (AuC), dentate gyrus (DG), CA1, CA3, and CA4. Data are means ± SEM. * P

    Journal: Cerebral Cortex (New York, NY)

    Article Title: Early Loss of Vision Results in Extensive Reorganization of Plasticity-Related Receptors and Alterations in Hippocampal Function That Extend Through Adulthood

    doi: 10.1093/cercor/bhy297

    Figure Lengend Snippet: GluN2B expression is globally increased in CBA/J mice 4 months postnatally. ( A ) Bar charts represent mean GluN2B receptor optical densities in different brain regions 4 months postnatally in CBA/J mice compared with (CBA/CaOlaHsd) controls. Receptor density is significantly increased in the piriform cortex (PiC), somatosensory cortex (SC), posterior parietal cortex (PPC), visual cortex (VC), auditory cortex (AuC), dentate gyrus (DG), CA1, CA3, and CA4. Data are means ± SEM. * P

    Article Snippet: The secondary antibody was applied for 90 min. We used a biotinylated horse–antimouse antibody for GABA-A and GABA-B (1:500, BA-2001, Vector Laboratories) and a biotinylated horse–antigoat antibody for GluN2B (1:500, BA-9500, Vector Laboratories).

    Techniques: Expressing, Crocin Bleaching Assay, Mouse Assay