biotinylated ha  (Sino Biological)


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    Name:
    Influenza A H1N1 A California 07 2009 Hemagglutinin HA Protein His Tag Biotinylated
    Description:
    A DNA sequence encoding the extracellular domain of Influenza A virus H1N1 A California 07 2009 ACP44189 1 hemagglutinin Met1 Gln529 HA1 HA2 two amino acids deletion uncleaved was fused with a polyhistidine tag at the C terminus The purified protein was biotinylated in vitro
    Catalog Number:
    11085-V08H-B
    Price:
    None
    Category:
    recombinant protein
    Host:
    HEK293 Cells
    Buy from Supplier


    Structured Review

    Sino Biological biotinylated ha
    FcγRIIIa dimer-binding assay. (A) Assay setup. Influenza HA protein (50 ng/well) was coated onto a 96-well plate before adding diluted plasma (containing IgGs). Subsequently, <t>biotinylated</t> FcγRIIIa dimers were added, and binding between
    A DNA sequence encoding the extracellular domain of Influenza A virus H1N1 A California 07 2009 ACP44189 1 hemagglutinin Met1 Gln529 HA1 HA2 two amino acids deletion uncleaved was fused with a polyhistidine tag at the C terminus The purified protein was biotinylated in vitro
    https://www.bioz.com/result/biotinylated ha/product/Sino Biological
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated ha - by Bioz Stars, 2021-06
    86/100 stars

    Images

    1) Product Images from "Antibody Responses with Fc-Mediated Functions after Vaccination of HIV-Infected Subjects with Trivalent Influenza Vaccine"

    Article Title: Antibody Responses with Fc-Mediated Functions after Vaccination of HIV-Infected Subjects with Trivalent Influenza Vaccine

    Journal: Journal of Virology

    doi: 10.1128/JVI.00285-16

    FcγRIIIa dimer-binding assay. (A) Assay setup. Influenza HA protein (50 ng/well) was coated onto a 96-well plate before adding diluted plasma (containing IgGs). Subsequently, biotinylated FcγRIIIa dimers were added, and binding between
    Figure Legend Snippet: FcγRIIIa dimer-binding assay. (A) Assay setup. Influenza HA protein (50 ng/well) was coated onto a 96-well plate before adding diluted plasma (containing IgGs). Subsequently, biotinylated FcγRIIIa dimers were added, and binding between

    Techniques Used: Binding Assay

    ADP assay with THP-1 monocytic cell line. (A) Assay setup. Fluorescently labeled beads were coated with both biotinylated HA from A/Switzerland/9715293/2013 (H3N2) and a fluorescent internalization probe and then opsonized with Abs and incubated with
    Figure Legend Snippet: ADP assay with THP-1 monocytic cell line. (A) Assay setup. Fluorescently labeled beads were coated with both biotinylated HA from A/Switzerland/9715293/2013 (H3N2) and a fluorescent internalization probe and then opsonized with Abs and incubated with

    Techniques Used: Labeling, Incubation

    2) Product Images from "Antibody Responses with Fc-Mediated Functions after Vaccination of HIV-Infected Subjects with Trivalent Influenza Vaccine"

    Article Title: Antibody Responses with Fc-Mediated Functions after Vaccination of HIV-Infected Subjects with Trivalent Influenza Vaccine

    Journal: Journal of Virology

    doi: 10.1128/JVI.00285-16

    FcγRIIIa dimer-binding assay. (A) Assay setup. Influenza HA protein (50 ng/well) was coated onto a 96-well plate before adding diluted plasma (containing IgGs). Subsequently, biotinylated FcγRIIIa dimers were added, and binding between
    Figure Legend Snippet: FcγRIIIa dimer-binding assay. (A) Assay setup. Influenza HA protein (50 ng/well) was coated onto a 96-well plate before adding diluted plasma (containing IgGs). Subsequently, biotinylated FcγRIIIa dimers were added, and binding between

    Techniques Used: Binding Assay

    ADP assay with THP-1 monocytic cell line. (A) Assay setup. Fluorescently labeled beads were coated with both biotinylated HA from A/Switzerland/9715293/2013 (H3N2) and a fluorescent internalization probe and then opsonized with Abs and incubated with
    Figure Legend Snippet: ADP assay with THP-1 monocytic cell line. (A) Assay setup. Fluorescently labeled beads were coated with both biotinylated HA from A/Switzerland/9715293/2013 (H3N2) and a fluorescent internalization probe and then opsonized with Abs and incubated with

    Techniques Used: Labeling, Incubation

    Related Articles

    other:

    Article Title: Study on the Antiviral Activities and Hemagglutinin-Based Molecular Mechanism of Novel Chlorogenin 3-O-β-Chacotrioside Derivatives against H5N1 Subtype Viruses
    Article Snippet: The balanced SSA-coated biosensor tips with biotinylated HA were immersed into the two-fold serial diluted compounds in black 96-well plates and carried out association for 45 s. Finally, the compounds were dissociated from the biotinylated proteins for 45 s. This experiment was repeated at least three times.

    Article Title: Study on the Antiviral Activities and Hemagglutinin-Based Molecular Mechanism of Novel Chlorogenin 3-O-β-Chacotrioside Derivatives against H5N1 Subtype Viruses
    Article Snippet: Dilute the biotinylated HA with PBST buffer to two-fold serial dilutions.

    Incubation:

    Article Title: Study on the Antiviral Activities and Hemagglutinin-Based Molecular Mechanism of Novel Chlorogenin 3-O-β-Chacotrioside Derivatives against H5N1 Subtype Viruses
    Article Snippet: Dilute the biotinylated HA with PBST buffer to two-fold serial dilutions. .. Afterwards, the diluted biotinylated HA was loaded onto the SSA biosensor tips and incubated for 5 min, followed by another equilibration. .. The balanced SSA-coated biosensor tips with biotinylated HA were immersed into the two-fold serial diluted compounds in black 96-well plates and carried out association for 45 s. Finally, the compounds were dissociated from the biotinylated proteins for 45 s. This experiment was repeated at least three times.

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  • 86
    Sino Biological biotinylated ha
    FcγRIIIa dimer-binding assay. (A) Assay setup. Influenza HA protein (50 ng/well) was coated onto a 96-well plate before adding diluted plasma (containing IgGs). Subsequently, <t>biotinylated</t> FcγRIIIa dimers were added, and binding between
    Biotinylated Ha, supplied by Sino Biological, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated ha/product/Sino Biological
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated ha - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    90
    Sino Biological influenza a h1n1
    FcγRIIIa dimer-binding assay. (A) Assay setup. Influenza HA protein (50 ng/well) was coated onto a 96-well plate before adding diluted plasma (containing IgGs). Subsequently, <t>biotinylated</t> FcγRIIIa dimers were added, and binding between
    Influenza A H1n1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/influenza a h1n1/product/Sino Biological
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    influenza a h1n1 - by Bioz Stars, 2021-06
    90/100 stars
      Buy from Supplier

    Image Search Results


    FcγRIIIa dimer-binding assay. (A) Assay setup. Influenza HA protein (50 ng/well) was coated onto a 96-well plate before adding diluted plasma (containing IgGs). Subsequently, biotinylated FcγRIIIa dimers were added, and binding between

    Journal: Journal of Virology

    Article Title: Antibody Responses with Fc-Mediated Functions after Vaccination of HIV-Infected Subjects with Trivalent Influenza Vaccine

    doi: 10.1128/JVI.00285-16

    Figure Lengend Snippet: FcγRIIIa dimer-binding assay. (A) Assay setup. Influenza HA protein (50 ng/well) was coated onto a 96-well plate before adding diluted plasma (containing IgGs). Subsequently, biotinylated FcγRIIIa dimers were added, and binding between

    Article Snippet: FIP-coated beads were incubated overnight with either 0.25 μg/μl biotinylated HA from A/Switzerland/9715293/2013 (H3N2) or biotinylated SIV gp120 (Sinobiological).

    Techniques: Binding Assay

    ADP assay with THP-1 monocytic cell line. (A) Assay setup. Fluorescently labeled beads were coated with both biotinylated HA from A/Switzerland/9715293/2013 (H3N2) and a fluorescent internalization probe and then opsonized with Abs and incubated with

    Journal: Journal of Virology

    Article Title: Antibody Responses with Fc-Mediated Functions after Vaccination of HIV-Infected Subjects with Trivalent Influenza Vaccine

    doi: 10.1128/JVI.00285-16

    Figure Lengend Snippet: ADP assay with THP-1 monocytic cell line. (A) Assay setup. Fluorescently labeled beads were coated with both biotinylated HA from A/Switzerland/9715293/2013 (H3N2) and a fluorescent internalization probe and then opsonized with Abs and incubated with

    Article Snippet: FIP-coated beads were incubated overnight with either 0.25 μg/μl biotinylated HA from A/Switzerland/9715293/2013 (H3N2) or biotinylated SIV gp120 (Sinobiological).

    Techniques: Labeling, Incubation