biotinylated goat anti rabbit igg secondary antibody  (Vector Laboratories)


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    Name:
    VECTASTAIN Elite ABC HRP Kit Peroxidase Rabbit IgG
    Description:
    VECTASTAIN Elite ABC Peroxidase Staining Kit has more than 50 000 citations to its credit and remains widely popular Based on the versatile avidin biotin complex interaction the system is modular and along with our selection of secondary antibodies can accommodate a wide array of primary antibody and tissue species Our ABC kits are economical and continue to be a staple product in any immunohistochemistry IHC and immunocytochemistry ICC laboratory Peroxidase substrates produce sharp dense precipitates with crisp localization These characteristics in conjunction with the high sensitivity and low background of the VECTASTAIN ABC systems make the peroxidase enzyme a preferred choice in many applications eg In neural tissue the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes VECTASTAIN Elite ABC SystemAdvanced avidin biotin technology The Elite ABC complex is smaller very uniform and highly active allowing more accessibility for binding to a biotinylated targetHighest sensitivity low background The VECTASTAIN Elite ABC system is the most sensitive avidin biotin complex based peroxidase system The Elite ABC series is approximately 5 times more sensitive than the original VECTASTAIN ABC Kit with the same low background Cost effective Higher sensitivity leads to lower cost per slide Available without Standard kit or with biotinylated species specific or universal secondary antibodies Available in Ready To Use R T U formats Prediluted stabilized working solutions of Elite ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN Elite ABC Staining Kit reagents VECTASTAIN Elite ABC Kit Components 3 ml normal goat serum1 ml of concentrated biotinylated goat anti rabbit IgG secondary antibody2 ml each of concentrated A and B reagents Sufficient reagents to stain approximately 500 1000 sections The Avidin Biotin Complex MethodThe VECTASTAIN ABC systems are extremely sensitive due to the form and number of active enzyme molecules associated with the preformed Avidin Biotinylated enzyme Complex This ABC complex takes advantage of two important properties of avidin 1 an extraordinarily high affinity for biotin over one million times higher than antibody for most antigens and 2 four biotin binding sites These properties allow macromolecular complexes ABCs to be formed by mixing Avidin DH Reagent A with its paired biotinylated enzyme Reagent B prior to use The ABC reagent once formed remains stable for many hours after formation and can be used for several days after preparation The VECTASTAIN ABC Reagent can be used to detect any molecule that is biotinylated This property gives the avidin biotin complex ABC method great versatility in the types of targets that can be detected as well as the types of applications in which it can be employed Biotinylated primary antibodies secondaries lectins neuronal tracers nucleic acids and ligands can be effectively visualized in applications such as Tissue stainingMultiple labeling Multiplex IHC Western blottingSouthern and northern blottingIn situ hybridization detection ISH Enzyme immunoassays ELISA Neuronal tracingAll applications benefit from the high sensitivity low background reproducibility and economy of the VECTASTAIN ABC system
    Catalog Number:
    pk-6101
    Price:
    None
    Category:
    Protein chromogenic detection reagents or kits or substrates
    Reactivity:
    Rabbit
    Size:
    1 Kit
    Buy from Supplier


    Structured Review

    Vector Laboratories biotinylated goat anti rabbit igg secondary antibody
    VECTASTAIN Elite ABC HRP Kit Peroxidase Rabbit IgG
    VECTASTAIN Elite ABC Peroxidase Staining Kit has more than 50 000 citations to its credit and remains widely popular Based on the versatile avidin biotin complex interaction the system is modular and along with our selection of secondary antibodies can accommodate a wide array of primary antibody and tissue species Our ABC kits are economical and continue to be a staple product in any immunohistochemistry IHC and immunocytochemistry ICC laboratory Peroxidase substrates produce sharp dense precipitates with crisp localization These characteristics in conjunction with the high sensitivity and low background of the VECTASTAIN ABC systems make the peroxidase enzyme a preferred choice in many applications eg In neural tissue the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes VECTASTAIN Elite ABC SystemAdvanced avidin biotin technology The Elite ABC complex is smaller very uniform and highly active allowing more accessibility for binding to a biotinylated targetHighest sensitivity low background The VECTASTAIN Elite ABC system is the most sensitive avidin biotin complex based peroxidase system The Elite ABC series is approximately 5 times more sensitive than the original VECTASTAIN ABC Kit with the same low background Cost effective Higher sensitivity leads to lower cost per slide Available without Standard kit or with biotinylated species specific or universal secondary antibodies Available in Ready To Use R T U formats Prediluted stabilized working solutions of Elite ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN Elite ABC Staining Kit reagents VECTASTAIN Elite ABC Kit Components 3 ml normal goat serum1 ml of concentrated biotinylated goat anti rabbit IgG secondary antibody2 ml each of concentrated A and B reagents Sufficient reagents to stain approximately 500 1000 sections The Avidin Biotin Complex MethodThe VECTASTAIN ABC systems are extremely sensitive due to the form and number of active enzyme molecules associated with the preformed Avidin Biotinylated enzyme Complex This ABC complex takes advantage of two important properties of avidin 1 an extraordinarily high affinity for biotin over one million times higher than antibody for most antigens and 2 four biotin binding sites These properties allow macromolecular complexes ABCs to be formed by mixing Avidin DH Reagent A with its paired biotinylated enzyme Reagent B prior to use The ABC reagent once formed remains stable for many hours after formation and can be used for several days after preparation The VECTASTAIN ABC Reagent can be used to detect any molecule that is biotinylated This property gives the avidin biotin complex ABC method great versatility in the types of targets that can be detected as well as the types of applications in which it can be employed Biotinylated primary antibodies secondaries lectins neuronal tracers nucleic acids and ligands can be effectively visualized in applications such as Tissue stainingMultiple labeling Multiplex IHC Western blottingSouthern and northern blottingIn situ hybridization detection ISH Enzyme immunoassays ELISA Neuronal tracingAll applications benefit from the high sensitivity low background reproducibility and economy of the VECTASTAIN ABC system
    https://www.bioz.com/result/biotinylated goat anti rabbit igg secondary antibody/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated goat anti rabbit igg secondary antibody - by Bioz Stars, 2021-03
    99/100 stars

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    Related Articles

    Staining:

    Article Title: Hypoxia-inducible Factor 1 Activation from aP2(Fabp4)-Cre Mediated Knockout of Von Hippel-Lindau Gene Leads to Embryonic Lethality
    Article Snippet: The slides were individually incubated with HIF-1α (Sigma, H6536) or VEGF monoclonal antibody (Santa Cruz Biotechnology, sc-7269) at 1/200 dilution in a humid chamber at 4°C overnight. .. Secondary antibody staining was carried out with a biotin-labeled horse anti-mouse antibody 1/200 dilution (Vector Laboratories, catalog number: BA-2000) for 2 hrs at room temperature, followed by incubation with a streptavidin-biotin horseradish peroxidase complex (Vector Laboratories, catalog number: PK-6101). .. Detection was done using DAB substrate (Vector: peroxide substrate kit, SK-4100) for 10 minutes.

    Article Title: Diverged Effects of Piperine on Testicular Development: Stimulating Leydig Cell Development but Inhibiting Spermatogenesis in Rats
    Article Snippet: .. Immunohistochemical staining kit (Vectastain Elite, ABC kit, PK-6101) was purchased from Vector Laboratories (Burlingame, CA, United States). .. The antibodies for DIOL in radioimmunoassay (RIA) were provided by Dr. D. T. Armstrong (Department of Obstetrics and Gynecology, University of Western Ontario, London, ON, Canada).

    Incubation:

    Article Title: Hypoxia-inducible Factor 1 Activation from aP2(Fabp4)-Cre Mediated Knockout of Von Hippel-Lindau Gene Leads to Embryonic Lethality
    Article Snippet: The slides were individually incubated with HIF-1α (Sigma, H6536) or VEGF monoclonal antibody (Santa Cruz Biotechnology, sc-7269) at 1/200 dilution in a humid chamber at 4°C overnight. .. Secondary antibody staining was carried out with a biotin-labeled horse anti-mouse antibody 1/200 dilution (Vector Laboratories, catalog number: BA-2000) for 2 hrs at room temperature, followed by incubation with a streptavidin-biotin horseradish peroxidase complex (Vector Laboratories, catalog number: PK-6101). .. Detection was done using DAB substrate (Vector: peroxide substrate kit, SK-4100) for 10 minutes.

    Article Title: β-Catenin, a Transcription Factor Activated by Canonical Wnt Signaling, Is Expressed in Sensory Neurons of Calves Latently Infected with Bovine Herpesvirus 1
    Article Snippet: Slides were incubated with β-catenin at 1:200 (ab6302; Abcam), MAML1 at 1:200 (12166; Cell Signaling), bICP0 antibody at 1:250, or ORF2 rabbit polyclonal antibody at a 1:250 dilution overnight in a humidified chamber at 4°C. .. The next day, slides were washed in 1× TBS and incubated in biotinylated goat anti-rabbit IgG (PK-6101; Vector Laboratories) for 30 min at room temperature in a humidified chamber. .. Avidin-biotinylated enzyme complex was added to the slides for 30 min of incubation at room temperature.

    Article Title: An Ultradian Feeding Schedule in Rats Affects Metabolic Gene Expression in Liver, Brown Adipose Tissue and Skeletal Muscle with Only Mild Effects on Circadian Clocks
    Article Snippet: We used rabbit polyclonal anti arginine-vasopressin (AVP) (1:8000, Truus, a gift from Dr. Ruud Buijs, Netherlands Institute for Brain Research, Amsterdam, The Netherlands). .. The sections were washed in TBS, then incubated (2 h at 4 °C) with biotinylated goat anti-rabbit IgG (1:500, PK6101; Vectastain Standard Elite ABC Kit Vector Laboratories, Inc., Burlingame, CA, USA) for AVP immunostaining. .. After this, sections were rinsed in TBS and incubated (2 h) in a solution containing avidin–biotin peroxidase complex (Vectastain Elite ABC kit; Vector Laboratories Inc.).

    Article Title: The Wnt Signaling Pathway Is Differentially Expressed during the Bovine Herpesvirus 1 Latency-Reactivation Cycle: Evidence That Two Protein Kinases Associated with Neuronal Survival, Akt3 and BMPR2, Are Expressed at Higher Levels during Latency
    Article Snippet: Slides were subsequently incubated with Akt3 antibody (1:500 dilution; 152157; Abcam), GNAQ antibody (1:450 dilution; ab75825), BMPR2 (1:400 dilution; MA5-15827; ThermoFisher), or DKK1 antibody (1:500 dilution; ab188597; Abcam) overnight in a humidified chamber at 4°C. .. The next day, slides were washed in 1× Tris-buffered saline (TBS) and incubated in biotinylated goat anti-rabbit IgG (PK-6101; Vector Laboratories) for 30 min at room temperature in a humidified chamber. .. Avidin-biotinylated enzyme complex was added to the slides for 30 min of incubation at room temperature.

    Article Title: Genetic Association and Expression Analyses of the Phosphatidylinositol-4-Phosphate 5-Kinase (PIP5K1C) Gene in Alcohol Use Disorder – Relevance for Pain Signaling and Alcohol Use
    Article Snippet: .. Sections were incubated in the primary antibody rabbit anti-PIP5K1C (VECTASTAIN Elite ABC HRP Kit (Peroxidase, Rabbit IgG), PK-6101, Vector Laboratories, Burlingame, CA) for two days at 4°C on a shaker. ..

    Article Title: Graphene quantum dots prevent α-synucleinopathy in Parkinson’s disease
    Article Snippet: Sections were incubated with rabbit polyclonal anti-TH (cat. no. NB300–19, 1:1,000, Novus Biologicals) or rabbit polyclonal anti-pS129-α-syn (cat. no. ab59264, 1:1,000, abcam) with 5% NDS, 0.1% TX-100 containing PBS blocking solution. .. The sections were then incubated with streptavidin-conjugated HRP and biotinylated secondary antibodies (cat. no. PK-6101, Vector Laboratories). .. Immune-positive signals were visualized using a DAB kit (cat. no. SK-4100, Vector Laboratories).

    Immunohistochemistry:

    Article Title: Mutant Kras- and p16-regulated NOX4 activation overcomes metabolic checkpoints in development of pancreatic ductal adenocarcinoma
    Article Snippet: Sections were stained with hematoxylin and eosin (HE) according to standard procedures as previously described . .. For IHC analysis of Ki67 and NOX4, standard procedures were carried out according to the manual (#PK-6101 and #PK-6102, Vector Laboratories). ..

    Article Title: Diverged Effects of Piperine on Testicular Development: Stimulating Leydig Cell Development but Inhibiting Spermatogenesis in Rats
    Article Snippet: .. Immunohistochemical staining kit (Vectastain Elite, ABC kit, PK-6101) was purchased from Vector Laboratories (Burlingame, CA, United States). .. The antibodies for DIOL in radioimmunoassay (RIA) were provided by Dr. D. T. Armstrong (Department of Obstetrics and Gynecology, University of Western Ontario, London, ON, Canada).

    Immunostaining:

    Article Title: An Ultradian Feeding Schedule in Rats Affects Metabolic Gene Expression in Liver, Brown Adipose Tissue and Skeletal Muscle with Only Mild Effects on Circadian Clocks
    Article Snippet: We used rabbit polyclonal anti arginine-vasopressin (AVP) (1:8000, Truus, a gift from Dr. Ruud Buijs, Netherlands Institute for Brain Research, Amsterdam, The Netherlands). .. The sections were washed in TBS, then incubated (2 h at 4 °C) with biotinylated goat anti-rabbit IgG (1:500, PK6101; Vectastain Standard Elite ABC Kit Vector Laboratories, Inc., Burlingame, CA, USA) for AVP immunostaining. .. After this, sections were rinsed in TBS and incubated (2 h) in a solution containing avidin–biotin peroxidase complex (Vectastain Elite ABC kit; Vector Laboratories Inc.).

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  • 97
    Vector Laboratories biotinylated rabbit anti goat secondary antibodies
    OXA- (A and B) and OX1R- (C and D) immunoreactivity in the human normal prostate. (A) Positive cells scattered along the monolayered epithelium of a prostatic follicle. (B) The majority of positive cells visible in this micrograph contain differently stained granular material in the apical portion of their cytoplasm. (C and D) Positive cells lined along the stratified epithelium of three follicles. Some of them are elongated in shape and completely filled with clusters of immunoreactive granules. <t>Avidin–biotin</t> immunohistochemical method. Scale bars: 20 μm.
    Biotinylated Rabbit Anti Goat Secondary Antibodies, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated rabbit anti goat secondary antibodies/product/Vector Laboratories
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated rabbit anti goat secondary antibodies - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

    97
    Vector Laboratories biotinylated goat anti rabbit igg
    Distribution of avian influenza virus NP in selected organs of inoculated birds. Distribution of influenza NP in brain ( A , E , I , M ), lung ( B , F , J , N ), spleen ( C , G , K , O ) and heart ( D , H , L , P ) of inoculated chickens of selected viruses at 4 dpi (except for H5N1_H4_T 327 K) as detected by immunohistochemistry using primary polyclonal rabbit anti-NP A/FPV/Rostock/34 antibody (1:750) and a secondary <t>biotinylated</t> goat anti-rabbit <t>IgG</t> (Vector Laboratories, Burlingame, CA, USA) antibody (1:200). 3-amino-9-ethyl-carbazol (red-brown); hematoxylin counterstain (blue); Nomarski contrast; bars A,B,D,E,F,H,I,J,L,M,N,P = 20 µm. Bars C,G,K,O = 50 µm.
    Biotinylated Goat Anti Rabbit Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated goat anti rabbit igg/product/Vector Laboratories
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated goat anti rabbit igg - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

    Image Search Results


    OXA- (A and B) and OX1R- (C and D) immunoreactivity in the human normal prostate. (A) Positive cells scattered along the monolayered epithelium of a prostatic follicle. (B) The majority of positive cells visible in this micrograph contain differently stained granular material in the apical portion of their cytoplasm. (C and D) Positive cells lined along the stratified epithelium of three follicles. Some of them are elongated in shape and completely filled with clusters of immunoreactive granules. Avidin–biotin immunohistochemical method. Scale bars: 20 μm.

    Journal: Journal of Anatomy

    Article Title: Expression of orexin A and its receptor 1 in the human prostate

    doi: 10.1111/joa.12030

    Figure Lengend Snippet: OXA- (A and B) and OX1R- (C and D) immunoreactivity in the human normal prostate. (A) Positive cells scattered along the monolayered epithelium of a prostatic follicle. (B) The majority of positive cells visible in this micrograph contain differently stained granular material in the apical portion of their cytoplasm. (C and D) Positive cells lined along the stratified epithelium of three follicles. Some of them are elongated in shape and completely filled with clusters of immunoreactive granules. Avidin–biotin immunohistochemical method. Scale bars: 20 μm.

    Article Snippet: Goat polyclonal anti-OXA (sc-8070) and anti-OX1R antibodies (sc-8073), their respective blocking peptides (sc-8070 P, sc-8073 P), and mouse monoclonal anti-chromogranin A (Chr A) antibody (sc-47714) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); rabbit polyclonal anti-prepro-orexin antibody (AB3096), its blocking peptide (AG774) and monoclonal anti-tubulin antibody (MAB1637) from Chemicon International (Temecula, CA, USA); biotinylated rabbit anti-goat secondary antibodies and avidin–biotin complex (PK-6105) from Vector Laboratories (Burlingame, CA, USA); horseradish peroxidase-conjugated anti-goat IgG (A-5420) and anti-rabbit IgG (A-0545) from Sigma Chemical (St Louis, MO, USA).

    Techniques: Staining, Avidin-Biotin Assay, Immunohistochemistry

    OXA- (A and B) and OX1R- (C and D) immunoreactivity in the human hyperplastic prostate. (A) The basal membrane of an intrafollicular, papillar-like structure is lined by a continuous row of intensely stained cells. (B) A peculiarity often shown by positive basal cells is the presence of a slender cytoplasmic extension (arrows) directed towards the follicular lumen and intermingled between the negative apical cells. (C) Almost all basal cells of the follicular epithelium contain OX1R-immunoreactive material, which completely fills their cytoplasm. (D) Invagination of a prostatic follicle completely lined by positive basal cells. The arrow points to a small cluster of low intensely stained apical cells close in contact with the follicular fluid. Avidin–biotin immunohistochemical method. Scale bars: 20 μm.

    Journal: Journal of Anatomy

    Article Title: Expression of orexin A and its receptor 1 in the human prostate

    doi: 10.1111/joa.12030

    Figure Lengend Snippet: OXA- (A and B) and OX1R- (C and D) immunoreactivity in the human hyperplastic prostate. (A) The basal membrane of an intrafollicular, papillar-like structure is lined by a continuous row of intensely stained cells. (B) A peculiarity often shown by positive basal cells is the presence of a slender cytoplasmic extension (arrows) directed towards the follicular lumen and intermingled between the negative apical cells. (C) Almost all basal cells of the follicular epithelium contain OX1R-immunoreactive material, which completely fills their cytoplasm. (D) Invagination of a prostatic follicle completely lined by positive basal cells. The arrow points to a small cluster of low intensely stained apical cells close in contact with the follicular fluid. Avidin–biotin immunohistochemical method. Scale bars: 20 μm.

    Article Snippet: Goat polyclonal anti-OXA (sc-8070) and anti-OX1R antibodies (sc-8073), their respective blocking peptides (sc-8070 P, sc-8073 P), and mouse monoclonal anti-chromogranin A (Chr A) antibody (sc-47714) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); rabbit polyclonal anti-prepro-orexin antibody (AB3096), its blocking peptide (AG774) and monoclonal anti-tubulin antibody (MAB1637) from Chemicon International (Temecula, CA, USA); biotinylated rabbit anti-goat secondary antibodies and avidin–biotin complex (PK-6105) from Vector Laboratories (Burlingame, CA, USA); horseradish peroxidase-conjugated anti-goat IgG (A-5420) and anti-rabbit IgG (A-0545) from Sigma Chemical (St Louis, MO, USA).

    Techniques: Staining, Avidin-Biotin Assay, Immunohistochemistry

    Distribution of avian influenza virus NP in selected organs of inoculated birds. Distribution of influenza NP in brain ( A , E , I , M ), lung ( B , F , J , N ), spleen ( C , G , K , O ) and heart ( D , H , L , P ) of inoculated chickens of selected viruses at 4 dpi (except for H5N1_H4_T 327 K) as detected by immunohistochemistry using primary polyclonal rabbit anti-NP A/FPV/Rostock/34 antibody (1:750) and a secondary biotinylated goat anti-rabbit IgG (Vector Laboratories, Burlingame, CA, USA) antibody (1:200). 3-amino-9-ethyl-carbazol (red-brown); hematoxylin counterstain (blue); Nomarski contrast; bars A,B,D,E,F,H,I,J,L,M,N,P = 20 µm. Bars C,G,K,O = 50 µm.

    Journal: International Journal of Molecular Sciences

    Article Title: Insertion of Basic Amino Acids in the Hemagglutinin Cleavage Site of H4N2 Avian Influenza Virus (AIV)—Reduced Virus Fitness in Chickens is Restored by Reassortment with Highly Pathogenic H5N1 AIV

    doi: 10.3390/ijms21072353

    Figure Lengend Snippet: Distribution of avian influenza virus NP in selected organs of inoculated birds. Distribution of influenza NP in brain ( A , E , I , M ), lung ( B , F , J , N ), spleen ( C , G , K , O ) and heart ( D , H , L , P ) of inoculated chickens of selected viruses at 4 dpi (except for H5N1_H4_T 327 K) as detected by immunohistochemistry using primary polyclonal rabbit anti-NP A/FPV/Rostock/34 antibody (1:750) and a secondary biotinylated goat anti-rabbit IgG (Vector Laboratories, Burlingame, CA, USA) antibody (1:200). 3-amino-9-ethyl-carbazol (red-brown); hematoxylin counterstain (blue); Nomarski contrast; bars A,B,D,E,F,H,I,J,L,M,N,P = 20 µm. Bars C,G,K,O = 50 µm.

    Article Snippet: Following sections were used for immunohistochemistry using the avidin–biotin–peroxidase complex method (Vector Laboratories Burlingame, CA, USA) with a primary polyclonal rabbit anti-NP antibody (1:750), and a secondary biotinylated goat anti-rabbit IgG (Vector Laboratories, Burlingame, CA, USA) antibody (1:200) as described [ , ].

    Techniques: Immunohistochemistry, Plasmid Preparation

    Sodium/iodide symporter (NIS) expression is primarily restricted to striated ducts in human salivary glands. (A) NIS is expressed at a high level along basolateral membranes of striated duct cells (S), with fewer intercalated (rectangle) and excretory (E) duct cells demonstrating lower NIS levels. Acinar (A) cells do not express NIS. Anti-human NIS antibody, avidin-biotin complex, DAB chromogen, hematoxylin counterstain, bar=25 μm. (B) Distribution and intensity of NIS immunoreactivity among intercalated (ID), striated (SD), and excretory (ED) ductal cells in normal human submandibular, parotid, and minor salivary glands. NIS expression is greater and more frequent in striated ducts, regardless of gland type.

    Journal: Thyroid

    Article Title: Modulation of Sodium/Iodide Symporter Expression in the Salivary Gland

    doi: 10.1089/thy.2012.0571

    Figure Lengend Snippet: Sodium/iodide symporter (NIS) expression is primarily restricted to striated ducts in human salivary glands. (A) NIS is expressed at a high level along basolateral membranes of striated duct cells (S), with fewer intercalated (rectangle) and excretory (E) duct cells demonstrating lower NIS levels. Acinar (A) cells do not express NIS. Anti-human NIS antibody, avidin-biotin complex, DAB chromogen, hematoxylin counterstain, bar=25 μm. (B) Distribution and intensity of NIS immunoreactivity among intercalated (ID), striated (SD), and excretory (ED) ductal cells in normal human submandibular, parotid, and minor salivary glands. NIS expression is greater and more frequent in striated ducts, regardless of gland type.

    Article Snippet: The secondary antibody used was a biotinylated goat anti-rabbit antibody (1:200, BA-1000, Vector Laboratories, Inc., Burlingame, CA) that was incubated for 20 minutes at room temperature.

    Techniques: Expressing, Avidin-Biotin Assay

    NIS expression is reduced in inflamed and neoplastic human salivary glands. (A) NIS expression in striated ducts is decreased in inflammatory salivary glands, as well as in benign and malignant neoplasms of ductal origin. (B) NIS immunoreactivity in inflamed (i, ii) and neoplastic (iii, iv) salivary tissue. NIS expression is variably decreased in striated ducts (S) of chronically inflamed salivary glands (i, ii) , particularly in ducts demonstrating goblet metaplasia (arrows). Neoplastic cells of Warthin's tumor (iii) and mucoepidermoid carcinoma (iv) demonstrate lower NIS level, with immunostaining intensity of 2+ and 1+, respectively. Anti-human NIS antibody, avidin-biotin complex, DAB chromogen, hematoxylin counterstain, bars=25 μm. F, periductal fibrosis; L, lymphocytic inflammation.

    Journal: Thyroid

    Article Title: Modulation of Sodium/Iodide Symporter Expression in the Salivary Gland

    doi: 10.1089/thy.2012.0571

    Figure Lengend Snippet: NIS expression is reduced in inflamed and neoplastic human salivary glands. (A) NIS expression in striated ducts is decreased in inflammatory salivary glands, as well as in benign and malignant neoplasms of ductal origin. (B) NIS immunoreactivity in inflamed (i, ii) and neoplastic (iii, iv) salivary tissue. NIS expression is variably decreased in striated ducts (S) of chronically inflamed salivary glands (i, ii) , particularly in ducts demonstrating goblet metaplasia (arrows). Neoplastic cells of Warthin's tumor (iii) and mucoepidermoid carcinoma (iv) demonstrate lower NIS level, with immunostaining intensity of 2+ and 1+, respectively. Anti-human NIS antibody, avidin-biotin complex, DAB chromogen, hematoxylin counterstain, bars=25 μm. F, periductal fibrosis; L, lymphocytic inflammation.

    Article Snippet: The secondary antibody used was a biotinylated goat anti-rabbit antibody (1:200, BA-1000, Vector Laboratories, Inc., Burlingame, CA) that was incubated for 20 minutes at room temperature.

    Techniques: Expressing, Immunostaining, Avidin-Biotin Assay

    Characterization of neural cells in mouse striatal cultures (A-G). (A) Phase contrast photomicrograph of striatal cells at 5-7 days in vitro . A majority of the cells are neurons; scale bar = 20 μm. (B) Astrocytes were characterized by large nuclei with multiple nucleoli and dispersed heterochromatin (Hoechst 33342 blue immunofluorescence) and glial fibrillary acidic (GFAP) immunofluorescence (Cy3 red fluorochrome); scale bar = 25 μm. (C-E) Subpopulations of striatal neurons possessed μ (C), κ (E), and to a lesser extent δ (not shown), opioid receptor immunoreactivity. D E show phase contrast and κ opioid receptor immunofluorescent images of the same cells; scale bar = 20 μm. (F-G) Triple-label-identification of neurons, astrocytes and microglia in striatal cultures. Neurons were identified using anti-PGP 9.5 indirect immunofluorescence (Alexa 488, green product), while astrocytes were labeled using anti-GFAP indirect immunofluorescence (Alexa 546, red product) and microglia were detected using IB 4 biotinylated isolectin-conjugated (via avidin) to Alexa 350 (blue fluorescent product) (arrow) (F). A majority of the cells were neurons; microglia comprised 0.7% of the total cells. (G) A phase-contrast photomicrograph of the same cells as in Fig. 1F, some of which were non-viable/degenerating (hatched arrows); PGP 9.5 immunoreactivity is retained by many degenerating neurons; scale bar = 20 μm.

    Journal: Neuroscience

    Article Title: Synergistic Neurotoxicity of Opioids and Human Immunodeficiency Virus-1 Tat Protein in Striatal Neurons In Vitro

    doi:

    Figure Lengend Snippet: Characterization of neural cells in mouse striatal cultures (A-G). (A) Phase contrast photomicrograph of striatal cells at 5-7 days in vitro . A majority of the cells are neurons; scale bar = 20 μm. (B) Astrocytes were characterized by large nuclei with multiple nucleoli and dispersed heterochromatin (Hoechst 33342 blue immunofluorescence) and glial fibrillary acidic (GFAP) immunofluorescence (Cy3 red fluorochrome); scale bar = 25 μm. (C-E) Subpopulations of striatal neurons possessed μ (C), κ (E), and to a lesser extent δ (not shown), opioid receptor immunoreactivity. D E show phase contrast and κ opioid receptor immunofluorescent images of the same cells; scale bar = 20 μm. (F-G) Triple-label-identification of neurons, astrocytes and microglia in striatal cultures. Neurons were identified using anti-PGP 9.5 indirect immunofluorescence (Alexa 488, green product), while astrocytes were labeled using anti-GFAP indirect immunofluorescence (Alexa 546, red product) and microglia were detected using IB 4 biotinylated isolectin-conjugated (via avidin) to Alexa 350 (blue fluorescent product) (arrow) (F). A majority of the cells were neurons; microglia comprised 0.7% of the total cells. (G) A phase-contrast photomicrograph of the same cells as in Fig. 1F, some of which were non-viable/degenerating (hatched arrows); PGP 9.5 immunoreactivity is retained by many degenerating neurons; scale bar = 20 μm.

    Article Snippet: - Secondary biotinylated goat anti-rabbit antibodies (1:250) were conjugated to avidin-peroxidase (Vectastain ABC kit, Vector Laboratories, Burlingame, CA) or Cy2-avidin (1:250; Amersham Life Science, Pittsburgh, PA).

    Techniques: In Vitro, Immunofluorescence, Labeling, Avidin-Biotin Assay