biotinylated goat anti rabbit igg antibody  (Vector Laboratories)


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    Name:
    Biotinylated Goat Anti Rabbit IgG Antibody
    Description:
    Biotinylated Goat Anit Rabbit IgG Antibody is prepared using proprietary immunization schedules that produce high affinity antibodies The antibodies are then purified by affinity chromatography and cross reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining ELISAs and blots Biotinylated Goat Anti Rabbit IgG H L is supplied in liquid format With some exceptions the recommended dilution for most applications is 1 200 H L indicates the antibody recognizes both heavy and light chains This antibody is included in the VECTASTAIN ABC kits
    Catalog Number:
    ba-1000
    Price:
    None
    Category:
    Antibodies
    Reactivity:
    Rabbit
    Size:
    1 5 mg
    Host:
    Goat
    Buy from Supplier


    Structured Review

    Vector Laboratories biotinylated goat anti rabbit igg antibody
    Biotinylated Goat Anti Rabbit IgG Antibody
    Biotinylated Goat Anit Rabbit IgG Antibody is prepared using proprietary immunization schedules that produce high affinity antibodies The antibodies are then purified by affinity chromatography and cross reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining ELISAs and blots Biotinylated Goat Anti Rabbit IgG H L is supplied in liquid format With some exceptions the recommended dilution for most applications is 1 200 H L indicates the antibody recognizes both heavy and light chains This antibody is included in the VECTASTAIN ABC kits
    https://www.bioz.com/result/biotinylated goat anti rabbit igg antibody/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated goat anti rabbit igg antibody - by Bioz Stars, 2021-03
    86/100 stars

    Images

    1) Product Images from "ILDR2 Is a Novel B7-like Protein That Negatively Regulates T Cell Responses"

    Article Title: ILDR2 Is a Novel B7-like Protein That Negatively Regulates T Cell Responses

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1700325

    ILDR2-Fc binds to activated T cells. Binding of ILDR2-hFc was analyzed on resting and activated human CD4 + ( A ) and CD8 + ( B ) T cells. Isolated human CD4 + or CD8 + . Cells were incubated with ILDR2-hFc or isotype control (human IgG1; Synagis) at 100 μg/ml, followed by staining with PE-conjugated anti–human IgG Fc, and evaluated by flow cytometry. The results, shown as line graphs of ILDR2-hFc and isotype control (human IgG1) binding, are representative of two independent experiments. ( C ) Binding kinetics of ILDR2-mFc to resting and activated mouse CD4 + T cells. CD4 + CD25 − . Binding of biotinylated ILDR2-mFc and isotype control (mIgG2a) was tested at different time points of T cell activation and evaluated by flow cytometry. Results are shown as line graphs of ILDR2-mFc and isotype control (mIgG2a) binding.
    Figure Legend Snippet: ILDR2-Fc binds to activated T cells. Binding of ILDR2-hFc was analyzed on resting and activated human CD4 + ( A ) and CD8 + ( B ) T cells. Isolated human CD4 + or CD8 + . Cells were incubated with ILDR2-hFc or isotype control (human IgG1; Synagis) at 100 μg/ml, followed by staining with PE-conjugated anti–human IgG Fc, and evaluated by flow cytometry. The results, shown as line graphs of ILDR2-hFc and isotype control (human IgG1) binding, are representative of two independent experiments. ( C ) Binding kinetics of ILDR2-mFc to resting and activated mouse CD4 + T cells. CD4 + CD25 − . Binding of biotinylated ILDR2-mFc and isotype control (mIgG2a) was tested at different time points of T cell activation and evaluated by flow cytometry. Results are shown as line graphs of ILDR2-mFc and isotype control (mIgG2a) binding.

    Techniques Used: Binding Assay, Isolation, Incubation, Staining, Flow Cytometry, Cytometry, Activation Assay

    Related Articles

    Avidin-Biotin Assay:

    Article Title: Synergistic Neurotoxicity of Opioids and Human Immunodeficiency Virus-1 Tat Protein in Striatal Neurons In Vitro
    Article Snippet: Rabbit anti-μ (MOR1; 1:2000), δ (DOR1; 1:2500), and κ (KOR1; 1:2000) affinity purified, polyclonal antisera (gift from Dr. Robert P. Elde) were used to detect μ, δ, and κ opioid receptors, respectively. .. - Secondary biotinylated goat anti-rabbit antibodies (1:250) were conjugated to avidin-peroxidase (Vectastain ABC kit, Vector Laboratories, Burlingame, CA) or Cy2-avidin (1:250; Amersham Life Science, Pittsburgh, PA). ..

    Article Title: Insertion of Basic Amino Acids in the Hemagglutinin Cleavage Site of H4N2 Avian Influenza Virus (AIV)—Reduced Virus Fitness in Chickens is Restored by Reassortment with Highly Pathogenic H5N1 AIV
    Article Snippet: .. Following sections were used for immunohistochemistry using the avidin–biotin–peroxidase complex method (Vector Laboratories Burlingame, CA, USA) with a primary polyclonal rabbit anti-NP antibody (1:750), and a secondary biotinylated goat anti-rabbit IgG (Vector Laboratories, Burlingame, CA, USA) antibody (1:200) as described [ , ]. ..

    Incubation:

    Article Title: Combinational therapy using hypothermia and the immunophilin ligand FK506 to target altered pial arteriolar reactivity, axonal damage, and blood-brain barrier dysfunction after traumatic brain injury in rat
    Article Snippet: The sections were preincubated for 1 hour in 10% normal goat serum with 0.2% Triton X in PBS and then incubated for 18 hours with the rabbit anti-C-APP (Invitrogen, Carlsbad, CA, USA) diluted 1:1,000 in 1% normal goat serum in PBS. .. Next, the sections were incubated for 1 hour with biotinylated goat anti-rabbit immunoglobulin G (IgG) (Vector Laboratories Inc., Burlingame, CA, USA) diluted 1:1,000 in 1% normal goat serum in PBS. .. The reaction product was visualized by incubation for 1 hour in avidin-biotinylated enzyme complex (Vectastain ABC kit, Vector Laboratories Inc.), followed by 0.05% diaminobenzidene, 0.01% H2 O2 , and 0.3% imidazole in 0.1% mL sodium phosphate buffer for 15 minutes.

    Article Title: Modulation of Sodium/Iodide Symporter Expression in the Salivary Gland
    Article Snippet: Slides were incubated with affinity purified rabbit anti-human NIS polyclonal antibody, p442NIS, 1:40 at room temperature for 60 minutes in a Dako Autostainer Immunostaining System, followed by avidin and biotin block using the Dako Biotin Blocking System (X0590) for 20 minutes. .. The secondary antibody used was a biotinylated goat anti-rabbit antibody (1:200, BA-1000, Vector Laboratories, Inc., Burlingame, CA) that was incubated for 20 minutes at room temperature. .. The detection system used was Vectastain Elite (PK-6100, Vector Laboratories, Inc.) used for 30 minutes.

    other:

    Article Title: Vasohibin as an endothelium-derived negative feedback regulator of angiogenesis
    Article Snippet: The materials used and their sources were as follows: VEGF165 and HGF (Sigma-Aldrich); FGF-2 and growth factor-reduced Matrigel (Collaborative Research Inc.); PlGF and TNF-α (R & D Systems Inc.); α-MEM, DMEM, and medium 199 (M199; Nissui Pharmaceutical Co.); endothelial basal medium (EBM) containing EC growth supplements (Clonetics Corp.); FBS (JRH Biosciences Inc.); Opti-MEM, Lipofectin, and Oligofectamine (Invitrogen Corp.); ISOGEN (Nippon Gene Co.); Hybond N+ membrane, nitrocellulose membranes, and protein A Sepharose (Amersham Biosciences); mouse anti–human CD31 mAb (DakoCytomation); rat anti–mouse CD31 Ab (Research Diagnostics Inc.); and biotin-conjugated anti–rabbit IgG (Vector Laboratories Inc.).

    Immunohistochemistry:

    Article Title: Insertion of Basic Amino Acids in the Hemagglutinin Cleavage Site of H4N2 Avian Influenza Virus (AIV)—Reduced Virus Fitness in Chickens is Restored by Reassortment with Highly Pathogenic H5N1 AIV
    Article Snippet: .. Following sections were used for immunohistochemistry using the avidin–biotin–peroxidase complex method (Vector Laboratories Burlingame, CA, USA) with a primary polyclonal rabbit anti-NP antibody (1:750), and a secondary biotinylated goat anti-rabbit IgG (Vector Laboratories, Burlingame, CA, USA) antibody (1:200) as described [ , ]. ..

    Next-Generation Sequencing:

    Article Title: Fluorescent Arc/Arg3.1 indicator mice: a versatile tool to study brain activity changes in vitro and in vivo
    Article Snippet: Coronal brain slices (50 μm) were cut with a vibrating microslicer (VT-1000, Leica) and subjected for immunohistochemical analysis of the endogenous Arc/Arg3.1 protein and the d4EGFP expressions by the protocol mentioned above. .. Briefly, slices were blocked with 10% NGS containing 0.1% Triton X-100 antibody in PBS for 1 hr and reacted with either anti-Arc (Arg3.1 antiserum, provided by Dietmar Kuhl, dilution 1:1,500) or anti-GFP (Molecular Probes, Cat# , dilution 1:2,000) rabbit antibody in 1% NGS containing PBS overnight at 4 C. After reaction with biotinylated anti-rabbit antibody (Vector laboratory, dilution 1:200), immunoreactions were visualized with the ABC elite kit (Vector laboratory) and DAB development procedure. .. Two month-old male Tg Arc/Arg3.1-d4EGFP mice (line 11; 21–24 g) received p-MPPI by i.p. injection at a dose 8.5 mg per kg between 8 and 9 a.m. (3 mice were used per experimental group); control mice were injected at the same time with saline.

    Generated:

    Article Title: Enhanced biocompatibility of CD47-functionalized vascular stents
    Article Snippet: Finally, Ultra TMB substrate (Thermo Scientific) was added per manufacturer recommendation to determine the relative concentrations of surface-immobilized biotin. .. The absolute values of CD47 immobilization density were obtained by plotting the OD data generated with the sets of foil samples to a standard OD curve obtained with escalating amounts of biotinylated goat anti-rabbit antibody (Vector Lab), disclosed to contain an average of 10 biotin groups per a 150 kDa molecule. .. In a separate experiment the contribution of non-covalent binding of CD47 to the PEI-PDT modified steel surfaces was assessed by omitting both the reduction of PDT groups with DTT and subsequent surface modification with sulfo-LC-SPDP.

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  • 97
    Vector Laboratories biotinylated anti rabbit antibody
    Increased d4EGFP expression in a mouse model of Alzheimer’s disease Representative images of DG hippocampus (coronal sections) from one month-old Tg Arc/Arg3.1-d4EGFP and Tg 5XFAD /Tg Arc/Arg3.1-d4EGFP littermate mice are shown after visualization of d4EGFP by DAB (A, B) or immunofluorescence (C,D). Note the increased number of and labeling intensity of d4EGFP-positive neurons in CA1 (B) and DG granule cells (B, D) in the Tg 5XFAD /Tg Arc/Arg3.1-d4EGFP mice. Brain sections stained with anti-GFP antibody and visualized by <t>biotinylated</t> (DAB method) or FITC-conjugated secondary antibodies. The fluorescent images are single confocal scans. Abbreviations: db – dorsal blade of DG, vb – ventral blade of DG; scale bars = 250 μm (A,B) and 100 μm (C,D). (E) Count of d4EGFP-positive neurons in Tg 5XFAD /Tg Arc/Arg3.1-d4EGFP mice, as percent change from control Tg Arc/Arg3.1-d4EGFP littermates, in the DG: (% mean ± SD): 216.3 ± 7.7 vs. 100 ± 6.4 (**p
    Biotinylated Anti Rabbit Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated anti rabbit antibody/product/Vector Laboratories
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated anti rabbit antibody - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

    97
    Vector Laboratories biotinylated rabbit anti goat secondary antibodies
    OXA- (A and B) and OX1R- (C and D) immunoreactivity in the human normal prostate. (A) Positive cells scattered along the monolayered epithelium of a prostatic follicle. (B) The majority of positive cells visible in this micrograph contain differently stained granular material in the apical portion of their cytoplasm. (C and D) Positive cells lined along the stratified epithelium of three follicles. Some of them are elongated in shape and completely filled with clusters of immunoreactive granules. <t>Avidin–biotin</t> immunohistochemical method. Scale bars: 20 μm.
    Biotinylated Rabbit Anti Goat Secondary Antibodies, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated rabbit anti goat secondary antibodies/product/Vector Laboratories
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated rabbit anti goat secondary antibodies - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

    Image Search Results


    Increased d4EGFP expression in a mouse model of Alzheimer’s disease Representative images of DG hippocampus (coronal sections) from one month-old Tg Arc/Arg3.1-d4EGFP and Tg 5XFAD /Tg Arc/Arg3.1-d4EGFP littermate mice are shown after visualization of d4EGFP by DAB (A, B) or immunofluorescence (C,D). Note the increased number of and labeling intensity of d4EGFP-positive neurons in CA1 (B) and DG granule cells (B, D) in the Tg 5XFAD /Tg Arc/Arg3.1-d4EGFP mice. Brain sections stained with anti-GFP antibody and visualized by biotinylated (DAB method) or FITC-conjugated secondary antibodies. The fluorescent images are single confocal scans. Abbreviations: db – dorsal blade of DG, vb – ventral blade of DG; scale bars = 250 μm (A,B) and 100 μm (C,D). (E) Count of d4EGFP-positive neurons in Tg 5XFAD /Tg Arc/Arg3.1-d4EGFP mice, as percent change from control Tg Arc/Arg3.1-d4EGFP littermates, in the DG: (% mean ± SD): 216.3 ± 7.7 vs. 100 ± 6.4 (**p

    Journal: Journal of neuroscience methods

    Article Title: Fluorescent Arc/Arg3.1 indicator mice: a versatile tool to study brain activity changes in vitro and in vivo

    doi: 10.1016/j.jneumeth.2009.07.015

    Figure Lengend Snippet: Increased d4EGFP expression in a mouse model of Alzheimer’s disease Representative images of DG hippocampus (coronal sections) from one month-old Tg Arc/Arg3.1-d4EGFP and Tg 5XFAD /Tg Arc/Arg3.1-d4EGFP littermate mice are shown after visualization of d4EGFP by DAB (A, B) or immunofluorescence (C,D). Note the increased number of and labeling intensity of d4EGFP-positive neurons in CA1 (B) and DG granule cells (B, D) in the Tg 5XFAD /Tg Arc/Arg3.1-d4EGFP mice. Brain sections stained with anti-GFP antibody and visualized by biotinylated (DAB method) or FITC-conjugated secondary antibodies. The fluorescent images are single confocal scans. Abbreviations: db – dorsal blade of DG, vb – ventral blade of DG; scale bars = 250 μm (A,B) and 100 μm (C,D). (E) Count of d4EGFP-positive neurons in Tg 5XFAD /Tg Arc/Arg3.1-d4EGFP mice, as percent change from control Tg Arc/Arg3.1-d4EGFP littermates, in the DG: (% mean ± SD): 216.3 ± 7.7 vs. 100 ± 6.4 (**p

    Article Snippet: Briefly, slices were blocked with 10% NGS containing 0.1% Triton X-100 antibody in PBS for 1 hr and reacted with either anti-Arc (Arg3.1 antiserum, provided by Dietmar Kuhl, dilution 1:1,500) or anti-GFP (Molecular Probes, Cat# , dilution 1:2,000) rabbit antibody in 1% NGS containing PBS overnight at 4 C. After reaction with biotinylated anti-rabbit antibody (Vector laboratory, dilution 1:200), immunoreactions were visualized with the ABC elite kit (Vector laboratory) and DAB development procedure.

    Techniques: Expressing, Mouse Assay, Immunofluorescence, Labeling, Staining

    KIAA1036 is preferentially expressed in ECs. ( A ) Expression of KIAA1036 in cultured cells. Cells were preincubated in 0.1% FCS/α-MEM for 12 hours and then stimulated with growth factors as follows: HUVECs with VEGF (1 nM), HASMCs with PDGF (1 nM), human fibroblasts with FGF-2 (2 nM), and keratinocytes with EGF (1 nM). Thereafter, total RNA was obtained and Northern blotting for vasohibin was performed. ( B ) Expression of KIAA1036 in vivo was examined by multiple-tissue Northern blot. ( C ) Localization of KIAA1036 protein in the placenta. Sections of human placenta were subjected to immunostaining. Anti_human CD31 mAb, anti_KIAA1036 mAb, or mouse IgG was used as the primary Ab. Scale bars: 100 μm. ( D ) Expression of KIAA1036 in human embryo. Northern blotting for vasohibin was performed using a human developmental total RNA Northern blot.

    Journal: Journal of Clinical Investigation

    Article Title: Vasohibin as an endothelium-derived negative feedback regulator of angiogenesis

    doi: 10.1172/JCI200421152

    Figure Lengend Snippet: KIAA1036 is preferentially expressed in ECs. ( A ) Expression of KIAA1036 in cultured cells. Cells were preincubated in 0.1% FCS/α-MEM for 12 hours and then stimulated with growth factors as follows: HUVECs with VEGF (1 nM), HASMCs with PDGF (1 nM), human fibroblasts with FGF-2 (2 nM), and keratinocytes with EGF (1 nM). Thereafter, total RNA was obtained and Northern blotting for vasohibin was performed. ( B ) Expression of KIAA1036 in vivo was examined by multiple-tissue Northern blot. ( C ) Localization of KIAA1036 protein in the placenta. Sections of human placenta were subjected to immunostaining. Anti_human CD31 mAb, anti_KIAA1036 mAb, or mouse IgG was used as the primary Ab. Scale bars: 100 μm. ( D ) Expression of KIAA1036 in human embryo. Northern blotting for vasohibin was performed using a human developmental total RNA Northern blot.

    Article Snippet: The materials used and their sources were as follows: VEGF165 and HGF (Sigma-Aldrich); FGF-2 and growth factor-reduced Matrigel (Collaborative Research Inc.); PlGF and TNF-α (R & D Systems Inc.); α-MEM, DMEM, and medium 199 (M199; Nissui Pharmaceutical Co.); endothelial basal medium (EBM) containing EC growth supplements (Clonetics Corp.); FBS (JRH Biosciences Inc.); Opti-MEM, Lipofectin, and Oligofectamine (Invitrogen Corp.); ISOGEN (Nippon Gene Co.); Hybond N+ membrane, nitrocellulose membranes, and protein A Sepharose (Amersham Biosciences); mouse anti–human CD31 mAb (DakoCytomation); rat anti–mouse CD31 Ab (Research Diagnostics Inc.); and biotin-conjugated anti–rabbit IgG (Vector Laboratories Inc.).

    Techniques: Expressing, Cell Culture, Northern Blot, In Vivo, Immunostaining

    CD47 Quantification. (A) The modification chemistry summarized in was utilized to facilitate quantification. Following PEI-PDT addition, primary amines were acetylated using sulfo-NHS-Acetate. recCD47 and pepCD47 were biotinylated using Sulfo-NHS-LC-LC-Biotin.

    Journal: Biomaterials

    Article Title: Enhanced biocompatibility of CD47-functionalized vascular stents

    doi: 10.1016/j.biomaterials.2016.02.008

    Figure Lengend Snippet: CD47 Quantification. (A) The modification chemistry summarized in was utilized to facilitate quantification. Following PEI-PDT addition, primary amines were acetylated using sulfo-NHS-Acetate. recCD47 and pepCD47 were biotinylated using Sulfo-NHS-LC-LC-Biotin.

    Article Snippet: The absolute values of CD47 immobilization density were obtained by plotting the OD data generated with the sets of foil samples to a standard OD curve obtained with escalating amounts of biotinylated goat anti-rabbit antibody (Vector Lab), disclosed to contain an average of 10 biotin groups per a 150 kDa molecule.

    Techniques: Modification

    OXA- (A and B) and OX1R- (C and D) immunoreactivity in the human normal prostate. (A) Positive cells scattered along the monolayered epithelium of a prostatic follicle. (B) The majority of positive cells visible in this micrograph contain differently stained granular material in the apical portion of their cytoplasm. (C and D) Positive cells lined along the stratified epithelium of three follicles. Some of them are elongated in shape and completely filled with clusters of immunoreactive granules. Avidin–biotin immunohistochemical method. Scale bars: 20 μm.

    Journal: Journal of Anatomy

    Article Title: Expression of orexin A and its receptor 1 in the human prostate

    doi: 10.1111/joa.12030

    Figure Lengend Snippet: OXA- (A and B) and OX1R- (C and D) immunoreactivity in the human normal prostate. (A) Positive cells scattered along the monolayered epithelium of a prostatic follicle. (B) The majority of positive cells visible in this micrograph contain differently stained granular material in the apical portion of their cytoplasm. (C and D) Positive cells lined along the stratified epithelium of three follicles. Some of them are elongated in shape and completely filled with clusters of immunoreactive granules. Avidin–biotin immunohistochemical method. Scale bars: 20 μm.

    Article Snippet: Goat polyclonal anti-OXA (sc-8070) and anti-OX1R antibodies (sc-8073), their respective blocking peptides (sc-8070 P, sc-8073 P), and mouse monoclonal anti-chromogranin A (Chr A) antibody (sc-47714) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); rabbit polyclonal anti-prepro-orexin antibody (AB3096), its blocking peptide (AG774) and monoclonal anti-tubulin antibody (MAB1637) from Chemicon International (Temecula, CA, USA); biotinylated rabbit anti-goat secondary antibodies and avidin–biotin complex (PK-6105) from Vector Laboratories (Burlingame, CA, USA); horseradish peroxidase-conjugated anti-goat IgG (A-5420) and anti-rabbit IgG (A-0545) from Sigma Chemical (St Louis, MO, USA).

    Techniques: Staining, Avidin-Biotin Assay, Immunohistochemistry

    OXA- (A and B) and OX1R- (C and D) immunoreactivity in the human hyperplastic prostate. (A) The basal membrane of an intrafollicular, papillar-like structure is lined by a continuous row of intensely stained cells. (B) A peculiarity often shown by positive basal cells is the presence of a slender cytoplasmic extension (arrows) directed towards the follicular lumen and intermingled between the negative apical cells. (C) Almost all basal cells of the follicular epithelium contain OX1R-immunoreactive material, which completely fills their cytoplasm. (D) Invagination of a prostatic follicle completely lined by positive basal cells. The arrow points to a small cluster of low intensely stained apical cells close in contact with the follicular fluid. Avidin–biotin immunohistochemical method. Scale bars: 20 μm.

    Journal: Journal of Anatomy

    Article Title: Expression of orexin A and its receptor 1 in the human prostate

    doi: 10.1111/joa.12030

    Figure Lengend Snippet: OXA- (A and B) and OX1R- (C and D) immunoreactivity in the human hyperplastic prostate. (A) The basal membrane of an intrafollicular, papillar-like structure is lined by a continuous row of intensely stained cells. (B) A peculiarity often shown by positive basal cells is the presence of a slender cytoplasmic extension (arrows) directed towards the follicular lumen and intermingled between the negative apical cells. (C) Almost all basal cells of the follicular epithelium contain OX1R-immunoreactive material, which completely fills their cytoplasm. (D) Invagination of a prostatic follicle completely lined by positive basal cells. The arrow points to a small cluster of low intensely stained apical cells close in contact with the follicular fluid. Avidin–biotin immunohistochemical method. Scale bars: 20 μm.

    Article Snippet: Goat polyclonal anti-OXA (sc-8070) and anti-OX1R antibodies (sc-8073), their respective blocking peptides (sc-8070 P, sc-8073 P), and mouse monoclonal anti-chromogranin A (Chr A) antibody (sc-47714) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); rabbit polyclonal anti-prepro-orexin antibody (AB3096), its blocking peptide (AG774) and monoclonal anti-tubulin antibody (MAB1637) from Chemicon International (Temecula, CA, USA); biotinylated rabbit anti-goat secondary antibodies and avidin–biotin complex (PK-6105) from Vector Laboratories (Burlingame, CA, USA); horseradish peroxidase-conjugated anti-goat IgG (A-5420) and anti-rabbit IgG (A-0545) from Sigma Chemical (St Louis, MO, USA).

    Techniques: Staining, Avidin-Biotin Assay, Immunohistochemistry