biotinylated goat anti mouse secondary antibody  (Vector Laboratories)


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    Name:
    Biotinylated Goat Anti Mouse IgG Antibody
    Description:
    Biotinylated Goat Anti Mouse IgG Antibody is prepared using proprietary immunization schedules that produce high affinity antibodies The antibodies are then purified by affinity chromatography and cross reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining ELISAs and blots Biotinylated Goat Anti Mouse IgG H L is supplied in solution With some exceptions the recommended dilution for most applications is 1 200 H L indicates the antibody recognizes both heavy and light chains
    Catalog Number:
    ba-9200
    Price:
    None
    Category:
    Antibodies
    Reactivity:
    Mouse
    Size:
    1 5 mg
    Host:
    Goat
    Buy from Supplier


    Structured Review

    Vector Laboratories biotinylated goat anti mouse secondary antibody
    Biotinylated Goat Anti Mouse IgG Antibody
    Biotinylated Goat Anti Mouse IgG Antibody is prepared using proprietary immunization schedules that produce high affinity antibodies The antibodies are then purified by affinity chromatography and cross reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining ELISAs and blots Biotinylated Goat Anti Mouse IgG H L is supplied in solution With some exceptions the recommended dilution for most applications is 1 200 H L indicates the antibody recognizes both heavy and light chains
    https://www.bioz.com/result/biotinylated goat anti mouse secondary antibody/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated goat anti mouse secondary antibody - by Bioz Stars, 2021-03
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Apolipoprotein E Inhibits Neointimal Hyperplasia after Arterial Injury in Mice
    Article Snippet: For the identification of smooth muscle cells and endothelial cells, sections were incubated overnight at 4°C with anti-smooth muscle α-actin (Clone 1A4; Sigma Chemical Co.) at 1:3,000 dilution or anti-Von Willebrand Factor (DAKO, Carpinteria, CA) at 1:100 dilution, respectively. .. The slides were washed three times for 15 minutes each with PBS containing Triton X-100 and then incubated for 1 hour at 23°C with 0.5% biotinylated anti-mouse IgG (Vector Laboratories, Inc., Burlingame, CA) for anti-smooth muscle cell α-actin or 0.5% biotinylated anti-rabbit IgG (Vector Laboratories, Inc.) for Von Willebrand Factor in the same solution containing 1.5% normal serum. .. Slides were then washed as described above, and then incubated with the avidin-peroxidase complex reagent (Peroxidase Vectastain Elite ABC kit, Vector Laboratories, Inc.) for 1 hour at 23°C.

    Article Title: Myocardial protection from ischemia/reperfusion injury by endogenous and exogenous HGF
    Article Snippet: Cell lysate was subjected to SDS-PAGE on a 12% polyacrylamide gel, and proteins were electroblotted on PVDF membrane (Bio-Rad Laboratories Inc., Hercules, California, USA). .. After blocking, the membrane was sequentially incubated with anti–phospho-p44/p42 mitogen-activated protein kinase (ERK-1/2) antibody (E10; New England BioLabs Inc., Beverly, Massachusetts, USA), biotinylated anti-mouse IgG (Vector Laboratories), horseradish peroxidase–conjugated streptavidin (Amersham Pharmacia Biotech UK, Little Chalfont, United Kingdom), and an enhanced chemiluminescence reagent (Amersham Pharmacia Biotech UK). .. To detect total ERK protein, the cell lysate was subjected to Western blot, as above except for use of an anti–ERK-1 antibody (K-23; Santa Cruz Biotechnology Inc.).

    Article Title: Pigment Epithelium-Derived Factor (PEDF) mediates cartilage matrix loss in an age-dependent manner under inflammatory conditions
    Article Snippet: For PEDF staining, sections were treated sequentially with both heat-mediated and enzymatic antigen retrieval using 10 mM Sodium Citrate, pH 6.0 and 0.3% hyaluronidase supplemented with 0.15% trypsin-EDTA, respectively. .. Samples were blocked in 10% goat serum in PBS, followed by mouse anti-PEDF (Chemicon, 10F12.2) incubation overnight, and biotinylated anti-mouse IgG (Vector Laboratories) incubation for 2 h. Primary antibody staining was visualized using the Vectastain ABC Kit with DAB Peroxidase Substrate Kit (Vector Laboratories). ..

    Article Title: α-catenin acts as a tumor suppressor in E-cadherin-negative basal-like breast cancer by inhibiting NF-κB signaling
    Article Snippet: To block endogenous peroxidase activity, the sections were incubated with 3% hydrogen peroxide for 10 min. After 1 h of preincubation in 5% normal goat serum to prevent nonspecific staining, the samples were incubated with the antibody to IκBα (1:50, Cell Signaling Technology, 4814) or TNFα (1:50, Novus Biologicals, NBP1-19532) at 4°C overnight. .. The sections were incubated with a biotinylated secondary antibody (1:500, biotinylated anti-rabbit IgG(H+L), Vector Laboratories, BA-1000 for TNFα; 1:500, biotinylated anti-mouse IgG(H+L), Vector Laboratories, BA-9200 for IκBα) and then incubated with an avidin-biotin peroxidase complex solution (Vector Laboratories, PK-6100) for 30 min at room temperature. .. Color was developed using the DAB (diaminobenzidine) Substrate Kit (BD Biosciences, 550880).

    Staining:

    Article Title: Subsets of Transgenic T Cells That Recognize CD1 Induce or Prevent Murine Lupus: Role of Cytokines
    Article Snippet: Counterstaining was performed with rabbit anti–mouse IgG antibody conjugated with FITC (DAKO, San Diego, CA). .. Positive samples were confirmed by staining with biotinylated affinity-purified goat anti–mouse IgG antibodies and counterstaining with streptavidin conjugated with FITC (Vector Laboratories, Burlingame, CA). .. None of 12 serum samples from untreated BALB/c or BALB/c nu /nu mice were positive.

    Article Title: Pigment Epithelium-Derived Factor (PEDF) mediates cartilage matrix loss in an age-dependent manner under inflammatory conditions
    Article Snippet: For PEDF staining, sections were treated sequentially with both heat-mediated and enzymatic antigen retrieval using 10 mM Sodium Citrate, pH 6.0 and 0.3% hyaluronidase supplemented with 0.15% trypsin-EDTA, respectively. .. Samples were blocked in 10% goat serum in PBS, followed by mouse anti-PEDF (Chemicon, 10F12.2) incubation overnight, and biotinylated anti-mouse IgG (Vector Laboratories) incubation for 2 h. Primary antibody staining was visualized using the Vectastain ABC Kit with DAB Peroxidase Substrate Kit (Vector Laboratories). ..

    Article Title: Pigment Epithelium-Derived Factor (PEDF) mediates cartilage matrix loss in an age-dependent manner under inflammatory conditions
    Article Snippet: Nuclei were stained with DAPI (Roche) in both cases. .. For colorimetric staining, a biotinylated anti-mouse IgG secondary antibody (Vector Laboratories) or a goat anti-rabbit IgG secondary antibody (Vector Laboratories) was used, followed by the Vectastain ABC Kit with DAB Peroxidase Substrate Kit (Vector Laboratories), and counterstained with 0.5% Methyl Green in 0.1 M Sodium Acetate. .. For PEDF and MMP-13 IHC, which involved the use of mouse antibodies, sections were blocked with M.O.M.

    Affinity Purification:

    Article Title: Subsets of Transgenic T Cells That Recognize CD1 Induce or Prevent Murine Lupus: Role of Cytokines
    Article Snippet: Counterstaining was performed with rabbit anti–mouse IgG antibody conjugated with FITC (DAKO, San Diego, CA). .. Positive samples were confirmed by staining with biotinylated affinity-purified goat anti–mouse IgG antibodies and counterstaining with streptavidin conjugated with FITC (Vector Laboratories, Burlingame, CA). .. None of 12 serum samples from untreated BALB/c or BALB/c nu /nu mice were positive.

    Blocking Assay:

    Article Title: Myocardial protection from ischemia/reperfusion injury by endogenous and exogenous HGF
    Article Snippet: Cell lysate was subjected to SDS-PAGE on a 12% polyacrylamide gel, and proteins were electroblotted on PVDF membrane (Bio-Rad Laboratories Inc., Hercules, California, USA). .. After blocking, the membrane was sequentially incubated with anti–phospho-p44/p42 mitogen-activated protein kinase (ERK-1/2) antibody (E10; New England BioLabs Inc., Beverly, Massachusetts, USA), biotinylated anti-mouse IgG (Vector Laboratories), horseradish peroxidase–conjugated streptavidin (Amersham Pharmacia Biotech UK, Little Chalfont, United Kingdom), and an enhanced chemiluminescence reagent (Amersham Pharmacia Biotech UK). .. To detect total ERK protein, the cell lysate was subjected to Western blot, as above except for use of an anti–ERK-1 antibody (K-23; Santa Cruz Biotechnology Inc.).

    Article Title: Potential role of orexin A binding the receptor 1 for orexins in normal and cryptorchid dogs
    Article Snippet: .. Antibodies and chemicals Mouse anti-OxA (MAB763) monoclonal antibody and its synthetic peptides were obtained from R & D Systems (Abingdon, UK) and from Tocris Bioscience (Bristol, UK), respectively; rabbit polyclonal anti-OX1R antibody (PAB8017) from Abnova (Taipan, Taiwan) and the synthetic blocking peptide (ab188501) from Abcam (Cambridge, UK); rabbit polyclonal anti-prepro-orexin antibody (AB3096), its blocking peptide (AG774) from Millipore (Temecula, CA, USA); monoclonal anti b-actin antibody (JLA20 CP01) from Calbiochem, San Diego, CA, USA; biotinylated goat anti-mouse (BA-9200) and goat anti-rabbit (BA-1000) secondary antibodies and avidin–biotin complex (PK-6105) from Vector Laboratories (Burlingame, CA, USA); horseradish peroxidase goat anti-rabbit IgG (A-0545) from the Sigma Chemical Co. (St. Louis, MO, USA); and marker proteins from Prosieve, Lonza, Rockland, ME, USA. .. The peptide OxA (003–30) was purchased from Phoenix Pharmaceuticals and the selective non-peptide orexin OX1R antagonist SB-408124 (1963) from Tocris Bioscience (Bristol, UK); the luteinizing hormone (LH) from sheep pituitary (L5269); EIA kit for testosterone determination from Adaltis (Bologna, Italy).

    Article Title: Potential role of orexin A binding the receptor 1 for orexins in normal and cryptorchid dogs
    Article Snippet: .. Mouse anti-OxA (MAB763) monoclonal antibody and its synthetic peptides were obtained from R & D Systems (Abingdon, UK) and from Tocris Bioscience (Bristol, UK), respectively; rabbit polyclonal anti-OX1R antibody (PAB8017) from Abnova (Taipan, Taiwan) and the synthetic blocking peptide (ab188501) from Abcam (Cambridge, UK); rabbit polyclonal anti-prepro-orexin antibody (AB3096), its blocking peptide (AG774) from Millipore (Temecula, CA, USA); monoclonal anti b-actin antibody (JLA20 CP01) from Calbiochem, San Diego, CA, USA; biotinylated goat anti-mouse (BA-9200) and goat anti-rabbit (BA-1000) secondary antibodies and avidin–biotin complex (PK-6105) from Vector Laboratories (Burlingame, CA, USA); horseradish peroxidase goat anti-rabbit IgG (A-0545) from the Sigma Chemical Co. (St. Louis, MO, USA); and marker proteins from Prosieve, Lonza, Rockland, ME, USA. .. The peptide OxA (003–30) was purchased from Phoenix Pharmaceuticals and the selective non-peptide orexin OX1R antagonist SB-408124 (1963) from Tocris Bioscience (Bristol, UK); the luteinizing hormone (LH) from sheep pituitary (L5269); EIA kit for testosterone determination from Adaltis (Bologna, Italy).

    Avidin-Biotin Assay:

    Article Title: Potential role of orexin A binding the receptor 1 for orexins in normal and cryptorchid dogs
    Article Snippet: .. Antibodies and chemicals Mouse anti-OxA (MAB763) monoclonal antibody and its synthetic peptides were obtained from R & D Systems (Abingdon, UK) and from Tocris Bioscience (Bristol, UK), respectively; rabbit polyclonal anti-OX1R antibody (PAB8017) from Abnova (Taipan, Taiwan) and the synthetic blocking peptide (ab188501) from Abcam (Cambridge, UK); rabbit polyclonal anti-prepro-orexin antibody (AB3096), its blocking peptide (AG774) from Millipore (Temecula, CA, USA); monoclonal anti b-actin antibody (JLA20 CP01) from Calbiochem, San Diego, CA, USA; biotinylated goat anti-mouse (BA-9200) and goat anti-rabbit (BA-1000) secondary antibodies and avidin–biotin complex (PK-6105) from Vector Laboratories (Burlingame, CA, USA); horseradish peroxidase goat anti-rabbit IgG (A-0545) from the Sigma Chemical Co. (St. Louis, MO, USA); and marker proteins from Prosieve, Lonza, Rockland, ME, USA. .. The peptide OxA (003–30) was purchased from Phoenix Pharmaceuticals and the selective non-peptide orexin OX1R antagonist SB-408124 (1963) from Tocris Bioscience (Bristol, UK); the luteinizing hormone (LH) from sheep pituitary (L5269); EIA kit for testosterone determination from Adaltis (Bologna, Italy).

    Article Title: Potential role of orexin A binding the receptor 1 for orexins in normal and cryptorchid dogs
    Article Snippet: .. Mouse anti-OxA (MAB763) monoclonal antibody and its synthetic peptides were obtained from R & D Systems (Abingdon, UK) and from Tocris Bioscience (Bristol, UK), respectively; rabbit polyclonal anti-OX1R antibody (PAB8017) from Abnova (Taipan, Taiwan) and the synthetic blocking peptide (ab188501) from Abcam (Cambridge, UK); rabbit polyclonal anti-prepro-orexin antibody (AB3096), its blocking peptide (AG774) from Millipore (Temecula, CA, USA); monoclonal anti b-actin antibody (JLA20 CP01) from Calbiochem, San Diego, CA, USA; biotinylated goat anti-mouse (BA-9200) and goat anti-rabbit (BA-1000) secondary antibodies and avidin–biotin complex (PK-6105) from Vector Laboratories (Burlingame, CA, USA); horseradish peroxidase goat anti-rabbit IgG (A-0545) from the Sigma Chemical Co. (St. Louis, MO, USA); and marker proteins from Prosieve, Lonza, Rockland, ME, USA. .. The peptide OxA (003–30) was purchased from Phoenix Pharmaceuticals and the selective non-peptide orexin OX1R antagonist SB-408124 (1963) from Tocris Bioscience (Bristol, UK); the luteinizing hormone (LH) from sheep pituitary (L5269); EIA kit for testosterone determination from Adaltis (Bologna, Italy).

    Article Title: α-catenin acts as a tumor suppressor in E-cadherin-negative basal-like breast cancer by inhibiting NF-κB signaling
    Article Snippet: To block endogenous peroxidase activity, the sections were incubated with 3% hydrogen peroxide for 10 min. After 1 h of preincubation in 5% normal goat serum to prevent nonspecific staining, the samples were incubated with the antibody to IκBα (1:50, Cell Signaling Technology, 4814) or TNFα (1:50, Novus Biologicals, NBP1-19532) at 4°C overnight. .. The sections were incubated with a biotinylated secondary antibody (1:500, biotinylated anti-rabbit IgG(H+L), Vector Laboratories, BA-1000 for TNFα; 1:500, biotinylated anti-mouse IgG(H+L), Vector Laboratories, BA-9200 for IκBα) and then incubated with an avidin-biotin peroxidase complex solution (Vector Laboratories, PK-6100) for 30 min at room temperature. .. Color was developed using the DAB (diaminobenzidine) Substrate Kit (BD Biosciences, 550880).

    Marker:

    Article Title: Potential role of orexin A binding the receptor 1 for orexins in normal and cryptorchid dogs
    Article Snippet: .. Antibodies and chemicals Mouse anti-OxA (MAB763) monoclonal antibody and its synthetic peptides were obtained from R & D Systems (Abingdon, UK) and from Tocris Bioscience (Bristol, UK), respectively; rabbit polyclonal anti-OX1R antibody (PAB8017) from Abnova (Taipan, Taiwan) and the synthetic blocking peptide (ab188501) from Abcam (Cambridge, UK); rabbit polyclonal anti-prepro-orexin antibody (AB3096), its blocking peptide (AG774) from Millipore (Temecula, CA, USA); monoclonal anti b-actin antibody (JLA20 CP01) from Calbiochem, San Diego, CA, USA; biotinylated goat anti-mouse (BA-9200) and goat anti-rabbit (BA-1000) secondary antibodies and avidin–biotin complex (PK-6105) from Vector Laboratories (Burlingame, CA, USA); horseradish peroxidase goat anti-rabbit IgG (A-0545) from the Sigma Chemical Co. (St. Louis, MO, USA); and marker proteins from Prosieve, Lonza, Rockland, ME, USA. .. The peptide OxA (003–30) was purchased from Phoenix Pharmaceuticals and the selective non-peptide orexin OX1R antagonist SB-408124 (1963) from Tocris Bioscience (Bristol, UK); the luteinizing hormone (LH) from sheep pituitary (L5269); EIA kit for testosterone determination from Adaltis (Bologna, Italy).

    Article Title: Potential role of orexin A binding the receptor 1 for orexins in normal and cryptorchid dogs
    Article Snippet: .. Mouse anti-OxA (MAB763) monoclonal antibody and its synthetic peptides were obtained from R & D Systems (Abingdon, UK) and from Tocris Bioscience (Bristol, UK), respectively; rabbit polyclonal anti-OX1R antibody (PAB8017) from Abnova (Taipan, Taiwan) and the synthetic blocking peptide (ab188501) from Abcam (Cambridge, UK); rabbit polyclonal anti-prepro-orexin antibody (AB3096), its blocking peptide (AG774) from Millipore (Temecula, CA, USA); monoclonal anti b-actin antibody (JLA20 CP01) from Calbiochem, San Diego, CA, USA; biotinylated goat anti-mouse (BA-9200) and goat anti-rabbit (BA-1000) secondary antibodies and avidin–biotin complex (PK-6105) from Vector Laboratories (Burlingame, CA, USA); horseradish peroxidase goat anti-rabbit IgG (A-0545) from the Sigma Chemical Co. (St. Louis, MO, USA); and marker proteins from Prosieve, Lonza, Rockland, ME, USA. .. The peptide OxA (003–30) was purchased from Phoenix Pharmaceuticals and the selective non-peptide orexin OX1R antagonist SB-408124 (1963) from Tocris Bioscience (Bristol, UK); the luteinizing hormone (LH) from sheep pituitary (L5269); EIA kit for testosterone determination from Adaltis (Bologna, Italy).

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    Vector Laboratories biotinylated secondary antibodies
    D 2 and D 3 immunocytochemistry were performed on the submucosal plexus of the ileum of CD-1 mice. The same tissue preparation from D 2 and D 3 KO mice was used as control. D 2 receptor immunoreactivity (IR) was revealed by a D 2 rabbit antibody and a donkey anti-rabbit Alexa 594 secondary antibody. D 3 receptor immunoreactivity was revealed by a D 3 goat antibody, a <t>biotinylated</t> donkey anti-goat secondary antibody, and streptavidin FITC. On the tissue of CD-1 mice, D 2 -immunoreactive products were present in the enteric neurons ( A ), and D 3 -immunoreactive products were also present in the enteric neurons ( B ). D 2 and D 3 immunoreactivities were colocalized in the same cell ( C ). However, D 2 immunoreactivity was not detected on the tissue of D 2 knock-out (KO) mice ( D ); no D 3 immunoreactivity was detected on the tissue of D 3 knock-out mouse ( E ). The arrows indicate the immunoreactive neurons. Scale bar: (in C ) 25 μm.
    Biotinylated Secondary Antibodies, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated secondary antibodies/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary antibodies - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    94
    Vector Laboratories biotinylated goat anti rat secondary antibody for cd107b
    Characterization of CeO 2 -induced macrophage recruitment and polarization in mice. Representative lung tissue sections of C57Bl/6 mice exposed to Saline or CeO 2 NP (5 or 50 μg, observation at 28 days), after immunostaining for <t>CD107b,</t> as a marker of total number of macrophages (Panel a ). Original magnification × 200. Quantification of CD107b positive cells (Panel b ). Representative lung tissue sections of mice exposed to Saline or CeO 2 NP (5 or 50 μg, observation at 28 days), after immunostaining for CD80 (Panel c and d for quantification), CD68 (Panel e and f for quantification), iNOS (Panel g and h for quantification), CD206 (Panel i and j for quantification), CD163 (Panel k and l for quantification) or Arginase 1 (Panel m and n for quantification). Original magnification × 200. Legends as in Fig. 4
    Biotinylated Goat Anti Rat Secondary Antibody For Cd107b, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated goat anti rat secondary antibody for cd107b/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated goat anti rat secondary antibody for cd107b - by Bioz Stars, 2021-03
    94/100 stars
      Buy from Supplier

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    D 2 and D 3 immunocytochemistry were performed on the submucosal plexus of the ileum of CD-1 mice. The same tissue preparation from D 2 and D 3 KO mice was used as control. D 2 receptor immunoreactivity (IR) was revealed by a D 2 rabbit antibody and a donkey anti-rabbit Alexa 594 secondary antibody. D 3 receptor immunoreactivity was revealed by a D 3 goat antibody, a biotinylated donkey anti-goat secondary antibody, and streptavidin FITC. On the tissue of CD-1 mice, D 2 -immunoreactive products were present in the enteric neurons ( A ), and D 3 -immunoreactive products were also present in the enteric neurons ( B ). D 2 and D 3 immunoreactivities were colocalized in the same cell ( C ). However, D 2 immunoreactivity was not detected on the tissue of D 2 knock-out (KO) mice ( D ); no D 3 immunoreactivity was detected on the tissue of D 3 knock-out mouse ( E ). The arrows indicate the immunoreactive neurons. Scale bar: (in C ) 25 μm.

    Journal: The Journal of Neuroscience

    Article Title: Physiological Modulation of Intestinal Motility by Enteric Dopaminergic Neurons and the D2 Receptor: Analysis of Dopamine Receptor Expression, Location, Development, and Function in Wild-Type and Knock-Out Mice

    doi: 10.1523/JNEUROSCI.4720-05.2006

    Figure Lengend Snippet: D 2 and D 3 immunocytochemistry were performed on the submucosal plexus of the ileum of CD-1 mice. The same tissue preparation from D 2 and D 3 KO mice was used as control. D 2 receptor immunoreactivity (IR) was revealed by a D 2 rabbit antibody and a donkey anti-rabbit Alexa 594 secondary antibody. D 3 receptor immunoreactivity was revealed by a D 3 goat antibody, a biotinylated donkey anti-goat secondary antibody, and streptavidin FITC. On the tissue of CD-1 mice, D 2 -immunoreactive products were present in the enteric neurons ( A ), and D 3 -immunoreactive products were also present in the enteric neurons ( B ). D 2 and D 3 immunoreactivities were colocalized in the same cell ( C ). However, D 2 immunoreactivity was not detected on the tissue of D 2 knock-out (KO) mice ( D ); no D 3 immunoreactivity was detected on the tissue of D 3 knock-out mouse ( E ). The arrows indicate the immunoreactive neurons. Scale bar: (in C ) 25 μm.

    Article Snippet: The blocked tissue was then incubated overnight with primary antibodies, washed with PBS, and exposed with intervening washes in PBS to the biotinylated secondary antibodies (2 h), 0.3% H2 O2 in 0.3% blocking sera (5 min), Elite ABC reagent (1 h; Vector Laboratories), and 3′,5′-diaminobenzidine solution (2–10 min) until suitable staining developed.

    Techniques: Immunocytochemistry, Mouse Assay, Knock-Out

    Avastin attenuates astrogliosis and blood vessel formation in the injured cortex. a, c Cortical sections were obtained from mice in which Avastin or PBS was infused into the ventricle 14 d post-ATP injection. GFAP and CoL1A1 were visualized with Alexa-488 and Alexa-594 conjugated secondary antibodies ( a ) or biotinylated secondary antibodies and DAB-based color reaction ( c ). b Relative fluorescence intensity of GFAP and CoL1A1 were measured using ZEN software and plotted. Values are means ± SEMs for animals treated with saline ( n = 4) or Avastin ( n = 3) (*, p

    Journal: Molecular Brain

    Article Title: Region-specific astrogliosis: differential vessel formation contributes to different patterns of astrogliosis in the cortex and striatum

    doi: 10.1186/s13041-020-00642-0

    Figure Lengend Snippet: Avastin attenuates astrogliosis and blood vessel formation in the injured cortex. a, c Cortical sections were obtained from mice in which Avastin or PBS was infused into the ventricle 14 d post-ATP injection. GFAP and CoL1A1 were visualized with Alexa-488 and Alexa-594 conjugated secondary antibodies ( a ) or biotinylated secondary antibodies and DAB-based color reaction ( c ). b Relative fluorescence intensity of GFAP and CoL1A1 were measured using ZEN software and plotted. Values are means ± SEMs for animals treated with saline ( n = 4) or Avastin ( n = 3) (*, p

    Article Snippet: The sections were rinsed three times with PBS and incubated with biotinylated secondary antibodies (Vector Laboratories, Burlingame, CA, USA).

    Techniques: Mouse Assay, Injection, Fluorescence, Software

    Expression of heme oxygenase-1 (HO-1) in WT and Sftpd −/− mice. Lung sections, prepared 72 h after exposure of 8-, 27-, and 80-wk-old WT and Sftpd −/− mice to air or ozone, were stained with antibody to HO-1 or IgG control followed by biotinylated secondary antibody. Binding was visualized by use of a peroxidase substrate DAB kit. One representative section from 3 separate experiments is shown ( n = 3 mice/treatment group). Original magnification, ×600.

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Age-related increases in ozone-induced injury and altered pulmonary mechanics in mice with progressive lung inflammation

    doi: 10.1152/ajplung.00027.2013

    Figure Lengend Snippet: Expression of heme oxygenase-1 (HO-1) in WT and Sftpd −/− mice. Lung sections, prepared 72 h after exposure of 8-, 27-, and 80-wk-old WT and Sftpd −/− mice to air or ozone, were stained with antibody to HO-1 or IgG control followed by biotinylated secondary antibody. Binding was visualized by use of a peroxidase substrate DAB kit. One representative section from 3 separate experiments is shown ( n = 3 mice/treatment group). Original magnification, ×600.

    Article Snippet: Sections were then incubated with biotinylated secondary antibody (1:200, Vector Labs, Burlingame, CA) for 30 min. A DAB Peroxidase Substrate Kit (Vector Labs) was used to visualize binding.

    Techniques: Expressing, Mouse Assay, Staining, Binding Assay

    Expression of mannose receptor in WT and Sftpd −/− mice. Lung sections, prepared 72 h after exposure of 8-, 27-, and 80-wk-old WT and Sftpd −/− mice to air or ozone, were stained with antibody to mannose receptor or IgG control followed by biotinylated secondary antibody. Binding was visualized by use of a peroxidase substrate DAB kit. One representative section from 3 separate experiments is shown ( n = 3 mice/treatment group). Original magnification, ×600.

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Age-related increases in ozone-induced injury and altered pulmonary mechanics in mice with progressive lung inflammation

    doi: 10.1152/ajplung.00027.2013

    Figure Lengend Snippet: Expression of mannose receptor in WT and Sftpd −/− mice. Lung sections, prepared 72 h after exposure of 8-, 27-, and 80-wk-old WT and Sftpd −/− mice to air or ozone, were stained with antibody to mannose receptor or IgG control followed by biotinylated secondary antibody. Binding was visualized by use of a peroxidase substrate DAB kit. One representative section from 3 separate experiments is shown ( n = 3 mice/treatment group). Original magnification, ×600.

    Article Snippet: Sections were then incubated with biotinylated secondary antibody (1:200, Vector Labs, Burlingame, CA) for 30 min. A DAB Peroxidase Substrate Kit (Vector Labs) was used to visualize binding.

    Techniques: Expressing, Mouse Assay, Staining, Binding Assay

    Expression of YM-1 in WT and Sftpd −/− mice. Lung sections, prepared 72 h after exposure of 8-, 27-, and 80-wk-old WT and Sftpd −/− mice to air or ozone, were stained with antibody to YM-1 or IgG control followed by biotinylated secondary antibody. Binding was visualized by use of a peroxidase substrate DAB kit. One representative section from 3 separate experiments is shown ( n = 3 mice/treatment group). Original magnification, ×600.

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Age-related increases in ozone-induced injury and altered pulmonary mechanics in mice with progressive lung inflammation

    doi: 10.1152/ajplung.00027.2013

    Figure Lengend Snippet: Expression of YM-1 in WT and Sftpd −/− mice. Lung sections, prepared 72 h after exposure of 8-, 27-, and 80-wk-old WT and Sftpd −/− mice to air or ozone, were stained with antibody to YM-1 or IgG control followed by biotinylated secondary antibody. Binding was visualized by use of a peroxidase substrate DAB kit. One representative section from 3 separate experiments is shown ( n = 3 mice/treatment group). Original magnification, ×600.

    Article Snippet: Sections were then incubated with biotinylated secondary antibody (1:200, Vector Labs, Burlingame, CA) for 30 min. A DAB Peroxidase Substrate Kit (Vector Labs) was used to visualize binding.

    Techniques: Expressing, Mouse Assay, Staining, Binding Assay

    Expression of galectin-3 in WT and Sftpd −/− mice. Lung sections, prepared 72 h after exposure of 8-, 27-, and 80-wk-old WT and Sftpd −/− mice to air or ozone, were stained with antibody to galectin-3 or IgG control followed by biotinylated secondary antibody. Binding was visualized by use of a peroxidase substrate DAB kit. One representative section from 3 separate experiments is shown ( n = 3 mice/treatment group). Original magnification, ×600.

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Age-related increases in ozone-induced injury and altered pulmonary mechanics in mice with progressive lung inflammation

    doi: 10.1152/ajplung.00027.2013

    Figure Lengend Snippet: Expression of galectin-3 in WT and Sftpd −/− mice. Lung sections, prepared 72 h after exposure of 8-, 27-, and 80-wk-old WT and Sftpd −/− mice to air or ozone, were stained with antibody to galectin-3 or IgG control followed by biotinylated secondary antibody. Binding was visualized by use of a peroxidase substrate DAB kit. One representative section from 3 separate experiments is shown ( n = 3 mice/treatment group). Original magnification, ×600.

    Article Snippet: Sections were then incubated with biotinylated secondary antibody (1:200, Vector Labs, Burlingame, CA) for 30 min. A DAB Peroxidase Substrate Kit (Vector Labs) was used to visualize binding.

    Techniques: Expressing, Mouse Assay, Staining, Binding Assay

    Expression of pro-surfactant protein C (SP-C) in WT and Sftpd −/− mice. Lung sections, prepared 72 h after exposure of 8-, 27-, and 80-wk-old WT and Sftpd −/− mice to air or ozone, were stained with antibody to pro-SP-C or IgG control followed by biotinylated secondary antibody. Binding was visualized by use of a peroxidase substrate DAB kit. One representative section from 3 separate experiments is shown ( n = 3 mice/treatment group). Arrowheads indicate insets. Original magnification, ×600; inset, ×1,000.

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Age-related increases in ozone-induced injury and altered pulmonary mechanics in mice with progressive lung inflammation

    doi: 10.1152/ajplung.00027.2013

    Figure Lengend Snippet: Expression of pro-surfactant protein C (SP-C) in WT and Sftpd −/− mice. Lung sections, prepared 72 h after exposure of 8-, 27-, and 80-wk-old WT and Sftpd −/− mice to air or ozone, were stained with antibody to pro-SP-C or IgG control followed by biotinylated secondary antibody. Binding was visualized by use of a peroxidase substrate DAB kit. One representative section from 3 separate experiments is shown ( n = 3 mice/treatment group). Arrowheads indicate insets. Original magnification, ×600; inset, ×1,000.

    Article Snippet: Sections were then incubated with biotinylated secondary antibody (1:200, Vector Labs, Burlingame, CA) for 30 min. A DAB Peroxidase Substrate Kit (Vector Labs) was used to visualize binding.

    Techniques: Expressing, Mouse Assay, Staining, Binding Assay

    Expression of inducible nitric oxide synthase (iNOS) in WT and Sftpd −/− mice. Lung sections, prepared 72 h after exposure of 8-, 27-, and 80-wk-old WT and Sftpd −/− mice to air or ozone, were stained with antibody to iNOS or IgG control followed by biotinylated secondary antibody. Binding was visualized by use of a peroxidase substrate DAB kit. One representative section from 3 separate experiments is shown ( n = 3 mice/treatment group). Original magnification, ×600.

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Age-related increases in ozone-induced injury and altered pulmonary mechanics in mice with progressive lung inflammation

    doi: 10.1152/ajplung.00027.2013

    Figure Lengend Snippet: Expression of inducible nitric oxide synthase (iNOS) in WT and Sftpd −/− mice. Lung sections, prepared 72 h after exposure of 8-, 27-, and 80-wk-old WT and Sftpd −/− mice to air or ozone, were stained with antibody to iNOS or IgG control followed by biotinylated secondary antibody. Binding was visualized by use of a peroxidase substrate DAB kit. One representative section from 3 separate experiments is shown ( n = 3 mice/treatment group). Original magnification, ×600.

    Article Snippet: Sections were then incubated with biotinylated secondary antibody (1:200, Vector Labs, Burlingame, CA) for 30 min. A DAB Peroxidase Substrate Kit (Vector Labs) was used to visualize binding.

    Techniques: Expressing, Mouse Assay, Staining, Binding Assay

    Characterization of CeO 2 -induced macrophage recruitment and polarization in mice. Representative lung tissue sections of C57Bl/6 mice exposed to Saline or CeO 2 NP (5 or 50 μg, observation at 28 days), after immunostaining for CD107b, as a marker of total number of macrophages (Panel a ). Original magnification × 200. Quantification of CD107b positive cells (Panel b ). Representative lung tissue sections of mice exposed to Saline or CeO 2 NP (5 or 50 μg, observation at 28 days), after immunostaining for CD80 (Panel c and d for quantification), CD68 (Panel e and f for quantification), iNOS (Panel g and h for quantification), CD206 (Panel i and j for quantification), CD163 (Panel k and l for quantification) or Arginase 1 (Panel m and n for quantification). Original magnification × 200. Legends as in Fig. 4

    Journal: Particle and Fibre Toxicology

    Article Title: Macrophage autophagy protects mice from cerium oxide nanoparticle-induced lung fibrosis

    doi: 10.1186/s12989-021-00398-y

    Figure Lengend Snippet: Characterization of CeO 2 -induced macrophage recruitment and polarization in mice. Representative lung tissue sections of C57Bl/6 mice exposed to Saline or CeO 2 NP (5 or 50 μg, observation at 28 days), after immunostaining for CD107b, as a marker of total number of macrophages (Panel a ). Original magnification × 200. Quantification of CD107b positive cells (Panel b ). Representative lung tissue sections of mice exposed to Saline or CeO 2 NP (5 or 50 μg, observation at 28 days), after immunostaining for CD80 (Panel c and d for quantification), CD68 (Panel e and f for quantification), iNOS (Panel g and h for quantification), CD206 (Panel i and j for quantification), CD163 (Panel k and l for quantification) or Arginase 1 (Panel m and n for quantification). Original magnification × 200. Legends as in Fig. 4

    Article Snippet: We used biotinylated goat anti-rabbit secondary antibodies for Collagen I, Collagen III, SMA, TGF-ß, CD68, CD80, iNOS, CD163, CD206, Arginase1 (Vector Labs), biotinylated goat anti-rat secondary antibody for CD107b (Vector Labs), horse anti-rabbit IgG (Vector Labs) for Atg5, Alexa Fluor 488 (Green) for LC3 and Alexa Fluor 546 (Red) secondary antibodies for LAMP1 (Invitrogen).

    Techniques: Mouse Assay, Immunostaining, Marker