biotinylated dextran amine (Thermo Fisher)
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Name:
Dextran Tetramethylrhodamine and biotin
Description:
Labeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division track the movement of live cells and to report the hydrodynamic properties of the cytoplasmic matrix The labeled dextran is commonly introduced into the cells via microinjection Need a different emission spectrum or longer tracking View our other mammalian cell tracking products Dextran Specifications • Label Ex Em Tetramethylrhodamine Biotin 555 580 • Size 10 000 MW• Charge Anionic• Fixable Fixable via LysineHigh Manufacturing Standards of Molecular Probes DextransWe offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges Dextrans are hydrophilic polysaccharides characterized by their moderate to high molecular weight good water solubility and low toxicity They also generally exhibit low immunogeniticy Dextrans are biologically inert due to their uncommon poly α D 1 6 glucose linkages which render them resistant to cleavage by most endogenous cellular glycosidases In most cases Molecular Probes fluorescent dextrans are much brighter and have higher negative charge than dextrans available from other sources Furthermore we use rigorous methods for removing as much unconjugated dye as practical and then assay our dextran conjugates by thin layer chromatography to help ensure the absence of low molecular weight contaminants A Wide Selection of Substituents and Molecular WeightsMolecular Probes dextrans are conjugated to biotin or a wide variety of fluorophores including seven of our Alexa Fluor dyes Molecular Probes dextran conjugates Table 14 4 and are available in these nominal molecular weights MW 3 000 10 000 40 000 70 000 500 000 and 2 000 000 daltons Dextran Net Charge and FixabilityWe employ succinimidyl coupling of our dyes to the dextran molecule which in most cases results in a neutral or anionic dextran The reaction used to produce the Rhodamine Green and Alexa Fluor 488 dextrans results in the final product being neutral anionic or cationic The Alexa Fluor Cascade Blue lucifer yellow fluorescein and Oregon Green dextrans are intrinsically anionic whereas most of the dextrans labeled with the zwitterionic rhodamine B tetramethylrhodamine and Texas Red dyes are essentially neutral To produce more highly anionic dextrans we have developed a proprietary procedure for adding negatively charged groups to the dextran carriers these products are designated polyanionic dextrans Some applications require that the dextran tracer be treated with formaldehyde or glutaraldehyde for subsequent analysis For these applications we offer lysine fixable versions of most of our dextran conjugates of fluorophores or biotin These dextrans have covalently bound lysine residues that permit dextran tracers to be conjugated to surrounding biomolecules by aldehyde mediated fixation for subsequent detection by immunohistochemical and ultrastructural techniques We have also shown that all of our 10 000 MW Alexa Fluor dextran conjugates can be fixed with aldehyde based fixatives Key Applications Using Labeled DextransThere are a multitude of citations describing the use of labeled dextrans Some of the most common uses include • Neuronal tracing anterograde and retrograde in live cells• Cell lineage tracing in live cells• Neuroanatomical tracing• Examining intercellular communications e g in gap junctions during wound healing and during embryonic development • Investigating vascular permeability and blood brain barrier integrity• Tracking endocytosis• Monitoring acidification some dextran dye conjugates are pH sensitive • Studying the hydrodynamic properties of the cytoplasmic matrixFor Research Use Only Not intended for any animal or human therapeutic or diagnostic use
Catalog Number:
d3312
Price:
None
Category:
Labeling Detection Products
Applications:
Cell Analysis|General Cell Tracing|Neuronal Tracing|Cell Tracing & Tracking
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Structured Review
Thermo Fisher
biotinylated dextran amine
Labeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division track the movement of live cells and to report the hydrodynamic properties of the cytoplasmic matrix The labeled dextran is commonly introduced into the cells via microinjection Need a different emission spectrum or longer tracking View our other mammalian cell tracking products Dextran Specifications • Label Ex Em Tetramethylrhodamine Biotin 555 580 • Size 10 000 MW• Charge Anionic• Fixable Fixable via LysineHigh Manufacturing Standards of Molecular Probes DextransWe offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges Dextrans are hydrophilic polysaccharides characterized by their moderate to high molecular weight good water solubility and low toxicity They also generally exhibit low immunogeniticy Dextrans are biologically inert due to their uncommon poly α D 1 6 glucose linkages which render them resistant to cleavage by most endogenous cellular glycosidases In most cases Molecular Probes fluorescent dextrans are much brighter and have higher negative charge than dextrans available from other sources Furthermore we use rigorous methods for removing as much unconjugated dye as practical and then assay our dextran conjugates by thin layer chromatography to help ensure the absence of low molecular weight contaminants A Wide Selection of Substituents and Molecular WeightsMolecular Probes dextrans are conjugated to biotin or a wide variety of fluorophores including seven of our Alexa Fluor dyes Molecular Probes dextran conjugates Table 14 4 and are available in these nominal molecular weights MW 3 000 10 000 40 000 70 000 500 000 and 2 000 000 daltons Dextran Net Charge and FixabilityWe employ succinimidyl coupling of our dyes to the dextran molecule which in most cases results in a neutral or anionic dextran The reaction used to produce the Rhodamine Green and Alexa Fluor 488 dextrans results in the final product being neutral anionic or cationic The Alexa Fluor Cascade Blue lucifer yellow fluorescein and Oregon Green dextrans are intrinsically anionic whereas most of the dextrans labeled with the zwitterionic rhodamine B tetramethylrhodamine and Texas Red dyes are essentially neutral To produce more highly anionic dextrans we have developed a proprietary procedure for adding negatively charged groups to the dextran carriers these products are designated polyanionic dextrans Some applications require that the dextran tracer be treated with formaldehyde or glutaraldehyde for subsequent analysis For these applications we offer lysine fixable versions of most of our dextran conjugates of fluorophores or biotin These dextrans have covalently bound lysine residues that permit dextran tracers to be conjugated to surrounding biomolecules by aldehyde mediated fixation for subsequent detection by immunohistochemical and ultrastructural techniques We have also shown that all of our 10 000 MW Alexa Fluor dextran conjugates can be fixed with aldehyde based fixatives Key Applications Using Labeled DextransThere are a multitude of citations describing the use of labeled dextrans Some of the most common uses include • Neuronal tracing anterograde and retrograde in live cells• Cell lineage tracing in live cells• Neuroanatomical tracing• Examining intercellular communications e g in gap junctions during wound healing and during embryonic development • Investigating vascular permeability and blood brain barrier integrity• Tracking endocytosis• Monitoring acidification some dextran dye conjugates are pH sensitive • Studying the hydrodynamic properties of the cytoplasmic matrixFor Research Use Only Not intended for any animal or human therapeutic or diagnostic use
https://www.bioz.com/result/biotinylated dextran amine/product/Thermo Fisher
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Labeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division track the movement of live cells and to report the hydrodynamic properties of the cytoplasmic matrix The labeled dextran is commonly introduced into the cells via microinjection Need a different emission spectrum or longer tracking View our other mammalian cell tracking products Dextran Specifications • Label Ex Em Tetramethylrhodamine Biotin 555 580 • Size 10 000 MW• Charge Anionic• Fixable Fixable via LysineHigh Manufacturing Standards of Molecular Probes DextransWe offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges Dextrans are hydrophilic polysaccharides characterized by their moderate to high molecular weight good water solubility and low toxicity They also generally exhibit low immunogeniticy Dextrans are biologically inert due to their uncommon poly α D 1 6 glucose linkages which render them resistant to cleavage by most endogenous cellular glycosidases In most cases Molecular Probes fluorescent dextrans are much brighter and have higher negative charge than dextrans available from other sources Furthermore we use rigorous methods for removing as much unconjugated dye as practical and then assay our dextran conjugates by thin layer chromatography to help ensure the absence of low molecular weight contaminants A Wide Selection of Substituents and Molecular WeightsMolecular Probes dextrans are conjugated to biotin or a wide variety of fluorophores including seven of our Alexa Fluor dyes Molecular Probes dextran conjugates Table 14 4 and are available in these nominal molecular weights MW 3 000 10 000 40 000 70 000 500 000 and 2 000 000 daltons Dextran Net Charge and FixabilityWe employ succinimidyl coupling of our dyes to the dextran molecule which in most cases results in a neutral or anionic dextran The reaction used to produce the Rhodamine Green and Alexa Fluor 488 dextrans results in the final product being neutral anionic or cationic The Alexa Fluor Cascade Blue lucifer yellow fluorescein and Oregon Green dextrans are intrinsically anionic whereas most of the dextrans labeled with the zwitterionic rhodamine B tetramethylrhodamine and Texas Red dyes are essentially neutral To produce more highly anionic dextrans we have developed a proprietary procedure for adding negatively charged groups to the dextran carriers these products are designated polyanionic dextrans Some applications require that the dextran tracer be treated with formaldehyde or glutaraldehyde for subsequent analysis For these applications we offer lysine fixable versions of most of our dextran conjugates of fluorophores or biotin These dextrans have covalently bound lysine residues that permit dextran tracers to be conjugated to surrounding biomolecules by aldehyde mediated fixation for subsequent detection by immunohistochemical and ultrastructural techniques We have also shown that all of our 10 000 MW Alexa Fluor dextran conjugates can be fixed with aldehyde based fixatives Key Applications Using Labeled DextransThere are a multitude of citations describing the use of labeled dextrans Some of the most common uses include • Neuronal tracing anterograde and retrograde in live cells• Cell lineage tracing in live cells• Neuroanatomical tracing• Examining intercellular communications e g in gap junctions during wound healing and during embryonic development • Investigating vascular permeability and blood brain barrier integrity• Tracking endocytosis• Monitoring acidification some dextran dye conjugates are pH sensitive • Studying the hydrodynamic properties of the cytoplasmic matrixFor Research Use Only Not intended for any animal or human therapeutic or diagnostic use
https://www.bioz.com/result/biotinylated dextran amine/product/Thermo Fisher
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
biotinylated dextran amine - by Bioz Stars,
2021-03
94/100 stars
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Labeling:Article Title: Functional and morphological effects of laser-induced ocular hypertension in retinas of adult albino Swiss mice Article Snippet: Previous studies in control mice indicate that OHSt application to both SCi, which are the main retinorecipient target regions in the brain, results in the labeling of 48,733 RGCs one week later; these represent 98.5% of the RGC population in albino Swiss mice [ ]. .. Retrograde labeling of retinal ganglion cells with Fluorescence:Article Title: Functional and morphological effects of laser-induced ocular hypertension in retinas of adult albino Swiss mice Article Snippet: Previous studies in control mice indicate that OHSt application to both SCi, which are the main retinorecipient target regions in the brain, results in the labeling of 48,733 RGCs one week later; these represent 98.5% of the RGC population in albino Swiss mice [ ]. .. Retrograde labeling of retinal ganglion cells with Transplantation Assay:Article Title: Angiomotin-like2 Gene (amotl2) Is Required for Migration and Proliferation of Endothelial Cells during Angiogenesis * Article Snippet: The injection doses were given in corresponding places. .. For cell transplantation, Tg(fli1:EGFP)y1 embryos were injected at the one-cell stage with 20 ng of Injection:Article Title: Angiomotin-like2 Gene (amotl2) Is Required for Migration and Proliferation of Endothelial Cells during Angiogenesis * Article Snippet: The injection doses were given in corresponding places. .. For cell transplantation, Tg(fli1:EGFP)y1 embryos were injected at the one-cell stage with 20 ng of |
