biotinylated dextran amine  (Thermo Fisher)


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    Name:
    Dextran Tetramethylrhodamine and biotin
    Description:
    Labeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division track the movement of live cells and to report the hydrodynamic properties of the cytoplasmic matrix The labeled dextran is commonly introduced into the cells via microinjection Need a different emission spectrum or longer tracking View our other mammalian cell tracking products Dextran Specifications • Label Ex Em Tetramethylrhodamine Biotin 555 580 • Size 10 000 MW• Charge Anionic• Fixable Fixable via LysineHigh Manufacturing Standards of Molecular Probes DextransWe offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges Dextrans are hydrophilic polysaccharides characterized by their moderate to high molecular weight good water solubility and low toxicity They also generally exhibit low immunogeniticy Dextrans are biologically inert due to their uncommon poly α D 1 6 glucose linkages which render them resistant to cleavage by most endogenous cellular glycosidases In most cases Molecular Probes fluorescent dextrans are much brighter and have higher negative charge than dextrans available from other sources Furthermore we use rigorous methods for removing as much unconjugated dye as practical and then assay our dextran conjugates by thin layer chromatography to help ensure the absence of low molecular weight contaminants A Wide Selection of Substituents and Molecular WeightsMolecular Probes dextrans are conjugated to biotin or a wide variety of fluorophores including seven of our Alexa Fluor dyes Molecular Probes dextran conjugates Table 14 4 and are available in these nominal molecular weights MW 3 000 10 000 40 000 70 000 500 000 and 2 000 000 daltons Dextran Net Charge and FixabilityWe employ succinimidyl coupling of our dyes to the dextran molecule which in most cases results in a neutral or anionic dextran The reaction used to produce the Rhodamine Green and Alexa Fluor 488 dextrans results in the final product being neutral anionic or cationic The Alexa Fluor Cascade Blue lucifer yellow fluorescein and Oregon Green dextrans are intrinsically anionic whereas most of the dextrans labeled with the zwitterionic rhodamine B tetramethylrhodamine and Texas Red dyes are essentially neutral To produce more highly anionic dextrans we have developed a proprietary procedure for adding negatively charged groups to the dextran carriers these products are designated polyanionic dextrans Some applications require that the dextran tracer be treated with formaldehyde or glutaraldehyde for subsequent analysis For these applications we offer lysine fixable versions of most of our dextran conjugates of fluorophores or biotin These dextrans have covalently bound lysine residues that permit dextran tracers to be conjugated to surrounding biomolecules by aldehyde mediated fixation for subsequent detection by immunohistochemical and ultrastructural techniques We have also shown that all of our 10 000 MW Alexa Fluor dextran conjugates can be fixed with aldehyde based fixatives Key Applications Using Labeled DextransThere are a multitude of citations describing the use of labeled dextrans Some of the most common uses include • Neuronal tracing anterograde and retrograde in live cells• Cell lineage tracing in live cells• Neuroanatomical tracing• Examining intercellular communications e g in gap junctions during wound healing and during embryonic development • Investigating vascular permeability and blood brain barrier integrity• Tracking endocytosis• Monitoring acidification some dextran dye conjugates are pH sensitive • Studying the hydrodynamic properties of the cytoplasmic matrixFor Research Use Only Not intended for any animal or human therapeutic or diagnostic use
    Catalog Number:
    d3312
    Price:
    None
    Category:
    Labeling Detection Products
    Applications:
    Cell Analysis|General Cell Tracing|Neuronal Tracing|Cell Tracing & Tracking
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    Structured Review

    Thermo Fisher biotinylated dextran amine
    Labeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division track the movement of live cells and to report the hydrodynamic properties of the cytoplasmic matrix The labeled dextran is commonly introduced into the cells via microinjection Need a different emission spectrum or longer tracking View our other mammalian cell tracking products Dextran Specifications • Label Ex Em Tetramethylrhodamine Biotin 555 580 • Size 10 000 MW• Charge Anionic• Fixable Fixable via LysineHigh Manufacturing Standards of Molecular Probes DextransWe offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges Dextrans are hydrophilic polysaccharides characterized by their moderate to high molecular weight good water solubility and low toxicity They also generally exhibit low immunogeniticy Dextrans are biologically inert due to their uncommon poly α D 1 6 glucose linkages which render them resistant to cleavage by most endogenous cellular glycosidases In most cases Molecular Probes fluorescent dextrans are much brighter and have higher negative charge than dextrans available from other sources Furthermore we use rigorous methods for removing as much unconjugated dye as practical and then assay our dextran conjugates by thin layer chromatography to help ensure the absence of low molecular weight contaminants A Wide Selection of Substituents and Molecular WeightsMolecular Probes dextrans are conjugated to biotin or a wide variety of fluorophores including seven of our Alexa Fluor dyes Molecular Probes dextran conjugates Table 14 4 and are available in these nominal molecular weights MW 3 000 10 000 40 000 70 000 500 000 and 2 000 000 daltons Dextran Net Charge and FixabilityWe employ succinimidyl coupling of our dyes to the dextran molecule which in most cases results in a neutral or anionic dextran The reaction used to produce the Rhodamine Green and Alexa Fluor 488 dextrans results in the final product being neutral anionic or cationic The Alexa Fluor Cascade Blue lucifer yellow fluorescein and Oregon Green dextrans are intrinsically anionic whereas most of the dextrans labeled with the zwitterionic rhodamine B tetramethylrhodamine and Texas Red dyes are essentially neutral To produce more highly anionic dextrans we have developed a proprietary procedure for adding negatively charged groups to the dextran carriers these products are designated polyanionic dextrans Some applications require that the dextran tracer be treated with formaldehyde or glutaraldehyde for subsequent analysis For these applications we offer lysine fixable versions of most of our dextran conjugates of fluorophores or biotin These dextrans have covalently bound lysine residues that permit dextran tracers to be conjugated to surrounding biomolecules by aldehyde mediated fixation for subsequent detection by immunohistochemical and ultrastructural techniques We have also shown that all of our 10 000 MW Alexa Fluor dextran conjugates can be fixed with aldehyde based fixatives Key Applications Using Labeled DextransThere are a multitude of citations describing the use of labeled dextrans Some of the most common uses include • Neuronal tracing anterograde and retrograde in live cells• Cell lineage tracing in live cells• Neuroanatomical tracing• Examining intercellular communications e g in gap junctions during wound healing and during embryonic development • Investigating vascular permeability and blood brain barrier integrity• Tracking endocytosis• Monitoring acidification some dextran dye conjugates are pH sensitive • Studying the hydrodynamic properties of the cytoplasmic matrixFor Research Use Only Not intended for any animal or human therapeutic or diagnostic use
    https://www.bioz.com/result/biotinylated dextran amine/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated dextran amine - by Bioz Stars, 2021-03
    94/100 stars

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    Related Articles

    Labeling:

    Article Title: Functional and morphological effects of laser-induced ocular hypertension in retinas of adult albino Swiss mice
    Article Snippet: Previous studies in control mice indicate that OHSt application to both SCi, which are the main retinorecipient target regions in the brain, results in the labeling of 48,733 RGCs one week later; these represent 98.5% of the RGC population in albino Swiss mice [ ]. .. Retrograde labeling of retinal ganglion cells with dextran tetramethylrhodamine To identify RGCs surviving retinal lasering with a competent axon at the level of the optic nerve (ON) head, the fluorescence tracer dextran tetramethylrhodamine (DTMR; 3,000 MW; Molecular Probes, Inc. Eugene, OR) was applied to the ocular stump of the intraorbitally transected left optic nerve 5 days after OHSt application and 2 days before sacrifice in groups II, III, and IV, following already described methods that are standard in our Laboratory [ , , , - ]. ..

    Fluorescence:

    Article Title: Functional and morphological effects of laser-induced ocular hypertension in retinas of adult albino Swiss mice
    Article Snippet: Previous studies in control mice indicate that OHSt application to both SCi, which are the main retinorecipient target regions in the brain, results in the labeling of 48,733 RGCs one week later; these represent 98.5% of the RGC population in albino Swiss mice [ ]. .. Retrograde labeling of retinal ganglion cells with dextran tetramethylrhodamine To identify RGCs surviving retinal lasering with a competent axon at the level of the optic nerve (ON) head, the fluorescence tracer dextran tetramethylrhodamine (DTMR; 3,000 MW; Molecular Probes, Inc. Eugene, OR) was applied to the ocular stump of the intraorbitally transected left optic nerve 5 days after OHSt application and 2 days before sacrifice in groups II, III, and IV, following already described methods that are standard in our Laboratory [ , , , - ]. ..

    Transplantation Assay:

    Article Title: Angiomotin-like2 Gene (amotl2) Is Required for Migration and Proliferation of Endothelial Cells during Angiogenesis *
    Article Snippet: The injection doses were given in corresponding places. .. For cell transplantation, Tg(fli1:EGFP)y1 embryos were injected at the one-cell stage with 20 ng of dextran tetramethylrhodamine (Molecular Probes) alone or in combination with amotl2-MO1 and used as donors at the sphere stage (about 4 hpf). .. When needed, host embryos were injected with amotl2-MO1 at the one-cell stage.

    Injection:

    Article Title: Angiomotin-like2 Gene (amotl2) Is Required for Migration and Proliferation of Endothelial Cells during Angiogenesis *
    Article Snippet: The injection doses were given in corresponding places. .. For cell transplantation, Tg(fli1:EGFP)y1 embryos were injected at the one-cell stage with 20 ng of dextran tetramethylrhodamine (Molecular Probes) alone or in combination with amotl2-MO1 and used as donors at the sphere stage (about 4 hpf). .. When needed, host embryos were injected with amotl2-MO1 at the one-cell stage.

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    Thermo Fisher miniruby
    Injection of an anterograde tracers, into the nucleus incertus (NI) and its transport to the amygdala. (A) <t>Miniruby</t> (mR) injection sites in the NI of cases sf2 and sf3. (B) Biotinylated dextran amine (BDA) injection sites in the NI in cases fh11 and fh15). (C) Site of mR injection in the NI of case sf2. (D) A darkfield image of the anterograde labeling in the ventromedial subnucleus of the lateral amygdala (LaVM) following a BDA injection into the NI (case fh11). (E) A collage of darkfield images of anterogradely-labeled fibers in amygdala at a mid-caudal level. Dense NI projections were present in the intra-amygdala part of the stria terminalis (STIA) and LaVM (case fh11). Calibration bars, 500 μm (A,B) , 100 μm (C) , 200 μm (D,E) .
    Miniruby, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/miniruby/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    miniruby - by Bioz Stars, 2021-03
    94/100 stars
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    95
    Thermo Fisher biotinylated dextran amine
    Corticocortical projections from SI barrel cortex terminate at MI sites located lateral to the sites most effective for evoking whisker movements. (A) Tangential section processed for cytochrome oxidase (CO) shows the spatial distribution of the layer IV barrels in SI cortex. (A′) An adjacent section processed for <t>biotinylated</t> dextran amine (BDA) shows the location of BDA deposits in SI barrel cortex. Contour lines indicate the primary (SI) and secondary (SII) somatosensory cortical areas as well as the posterior parietal cortex (PPC). Rectangle indicates the region depicted in panel (B) . (B) Location of two electrolytic lesions (red arrows) marking where intracranial microstimulation (ICMS) was most effective in evoking whisker twitches. Labeled projections (arrowheads) from SI terminate in a strip of MI cortex located caudal and lateral to sites that evoked the best whisker responses. Scale bars: 2 mm in (A) ; 500 μm in (B) .
    Biotinylated Dextran Amine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated dextran amine/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated dextran amine - by Bioz Stars, 2021-03
    95/100 stars
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    Image Search Results


    Injection of an anterograde tracers, into the nucleus incertus (NI) and its transport to the amygdala. (A) Miniruby (mR) injection sites in the NI of cases sf2 and sf3. (B) Biotinylated dextran amine (BDA) injection sites in the NI in cases fh11 and fh15). (C) Site of mR injection in the NI of case sf2. (D) A darkfield image of the anterograde labeling in the ventromedial subnucleus of the lateral amygdala (LaVM) following a BDA injection into the NI (case fh11). (E) A collage of darkfield images of anterogradely-labeled fibers in amygdala at a mid-caudal level. Dense NI projections were present in the intra-amygdala part of the stria terminalis (STIA) and LaVM (case fh11). Calibration bars, 500 μm (A,B) , 100 μm (C) , 200 μm (D,E) .

    Journal: Frontiers in Neuroanatomy

    Article Title: Comparative Distribution of Relaxin-3 Inputs and Calcium-Binding Protein-Positive Neurons in Rat Amygdala

    doi: 10.3389/fnana.2016.00036

    Figure Lengend Snippet: Injection of an anterograde tracers, into the nucleus incertus (NI) and its transport to the amygdala. (A) Miniruby (mR) injection sites in the NI of cases sf2 and sf3. (B) Biotinylated dextran amine (BDA) injection sites in the NI in cases fh11 and fh15). (C) Site of mR injection in the NI of case sf2. (D) A darkfield image of the anterograde labeling in the ventromedial subnucleus of the lateral amygdala (LaVM) following a BDA injection into the NI (case fh11). (E) A collage of darkfield images of anterogradely-labeled fibers in amygdala at a mid-caudal level. Dense NI projections were present in the intra-amygdala part of the stria terminalis (STIA) and LaVM (case fh11). Calibration bars, 500 μm (A,B) , 100 μm (C) , 200 μm (D,E) .

    Article Snippet: For anterograde tracing, 15% miniruby (mR, 10 kD biotinylated dextran amine (BDA) rhodamine-labeled; Molecular Probes, Paisley, UK; n = 2, Figure ) or biotinylated dextran amine (BDA, 10 kD; Molecular Probes, Eugene, OR, USA; n = 2, Figure ) dissolved in 0.1 M phosphate buffer (PB), pH 7.6 was iontophoretically delivered into the NI by passing a positive current of 1 μA, 2 s on 2 s off through the micropipette, over 10 min.

    Techniques: Injection, Labeling

    Corticocortical projections from SI barrel cortex terminate at MI sites located lateral to the sites most effective for evoking whisker movements. (A) Tangential section processed for cytochrome oxidase (CO) shows the spatial distribution of the layer IV barrels in SI cortex. (A′) An adjacent section processed for biotinylated dextran amine (BDA) shows the location of BDA deposits in SI barrel cortex. Contour lines indicate the primary (SI) and secondary (SII) somatosensory cortical areas as well as the posterior parietal cortex (PPC). Rectangle indicates the region depicted in panel (B) . (B) Location of two electrolytic lesions (red arrows) marking where intracranial microstimulation (ICMS) was most effective in evoking whisker twitches. Labeled projections (arrowheads) from SI terminate in a strip of MI cortex located caudal and lateral to sites that evoked the best whisker responses. Scale bars: 2 mm in (A) ; 500 μm in (B) .

    Journal: Frontiers in Neural Circuits

    Article Title: Rat whisker motor cortex is subdivided into sensory-input and motor-output areas

    doi: 10.3389/fncir.2013.00004

    Figure Lengend Snippet: Corticocortical projections from SI barrel cortex terminate at MI sites located lateral to the sites most effective for evoking whisker movements. (A) Tangential section processed for cytochrome oxidase (CO) shows the spatial distribution of the layer IV barrels in SI cortex. (A′) An adjacent section processed for biotinylated dextran amine (BDA) shows the location of BDA deposits in SI barrel cortex. Contour lines indicate the primary (SI) and secondary (SII) somatosensory cortical areas as well as the posterior parietal cortex (PPC). Rectangle indicates the region depicted in panel (B) . (B) Location of two electrolytic lesions (red arrows) marking where intracranial microstimulation (ICMS) was most effective in evoking whisker twitches. Labeled projections (arrowheads) from SI terminate in a strip of MI cortex located caudal and lateral to sites that evoked the best whisker responses. Scale bars: 2 mm in (A) ; 500 μm in (B) .

    Article Snippet: The tracers were either a 15% solution of biotinylated dextran amine (BDA, Invitrogen) or a 15% solution of Fluoro-Ruby (FR, Invitrogen).

    Techniques: Whisker Assay, Labeling, Stripping Membranes

    Injections of biotinylated dextrans into the ELa label small cell axons into the ELp. A , Overview of the ELa and injection site. At this dorsal level, the ELp is not visible. The injection site is ∼150 μm in diameter. B , Section ∼450 μm ventral to A . The border between the ELa and the ELp is somewhat indistinct toward the center ( narrow dotted line ), where the small cell axons enter the ELp. The boxed areas are magnified in C and D. C , Medial small cell fibers in the ELp. The fibers travel mostly radially toward the medial edge of the ELp. D , Posterior small cell fibers in the ELp. The fibers travel mostly radially toward the back of the ELp. E , Summary of two experiments in which biotinylated dextrans were injected into the ELa. i – viii , Horizontal sections of the ELa and the ELp over a dorsal–ventral series. The approximate positions of the sections are indicated in the inset . Figure legend continues. Circles in the ELa represent the injection sites, and fibers in the ELp are the resulting small cell projection. In the first experiment, the lateral injection ( dotted circle ) gave rise to lateral fibers ( dotted ). In the second experiment, the medial injection ( solid circle ) gave rise to the medial fibers ( solid ). L , Lateral toral nucleus; MD , medial dorsal nucleus; OT , optic tectum; Tel , telencephalon; VA , valvula. Scale bars: A , 800 μm; B , 200 μm; C, D , 50 μm; E , 500 μm for reconstructions, 1 mm for the inset .

    Journal: The Journal of Neuroscience

    Article Title: Neural Substrates for Species Recognition in the Time-Coding Electrosensory Pathway of Mormyrid Electric Fish

    doi: 10.1523/JNEUROSCI.18-03-01171.1998

    Figure Lengend Snippet: Injections of biotinylated dextrans into the ELa label small cell axons into the ELp. A , Overview of the ELa and injection site. At this dorsal level, the ELp is not visible. The injection site is ∼150 μm in diameter. B , Section ∼450 μm ventral to A . The border between the ELa and the ELp is somewhat indistinct toward the center ( narrow dotted line ), where the small cell axons enter the ELp. The boxed areas are magnified in C and D. C , Medial small cell fibers in the ELp. The fibers travel mostly radially toward the medial edge of the ELp. D , Posterior small cell fibers in the ELp. The fibers travel mostly radially toward the back of the ELp. E , Summary of two experiments in which biotinylated dextrans were injected into the ELa. i – viii , Horizontal sections of the ELa and the ELp over a dorsal–ventral series. The approximate positions of the sections are indicated in the inset . Figure legend continues. Circles in the ELa represent the injection sites, and fibers in the ELp are the resulting small cell projection. In the first experiment, the lateral injection ( dotted circle ) gave rise to lateral fibers ( dotted ). In the second experiment, the medial injection ( solid circle ) gave rise to the medial fibers ( solid ). L , Lateral toral nucleus; MD , medial dorsal nucleus; OT , optic tectum; Tel , telencephalon; VA , valvula. Scale bars: A , 800 μm; B , 200 μm; C, D , 50 μm; E , 500 μm for reconstructions, 1 mm for the inset .

    Article Snippet: We used broken-tipped microelectrodes (8–12 μm), filled with M r 3000 or 10,000 biotinylated dextran amines [Molecular Probes, Eugene, OR; 7% in 0.7 m KCl and 0.03% Triton X-100 (Aldrich, St. Louis, MO)].

    Techniques: Injection

    Injections of biotinylated dextrans into the ELp label small cell bodies in the ELa. A , Injection site in the ELp. The injection site ( ij ) is fairly anterior. Small cell bodies are labeled in the ELa, visible as dark spots . B , Close-up of the boxed area in A showing six small cell somata. C , Section of the ELp 100 μm dorsal to the injection site in A . Fibers projecting radially to the edge of the ELp are more easily visible here. D , Summary of two experiments injecting biotinylated dextrans into the ELp. i – viii , Horizontal sections of the ELa and the ELp over a dorsal–ventral series. The approximate positions of the sections are indicated in the inset . Circles in the ELp represent the injection sites, and symbols in the ELa are the positions of retrogradely labeled small cell somata. In the first experiment, the lateral injection ( solid circle ) retrogradely labels lateral small cell somata (○). In the second experiment, the medial injection ( dashed circle with + in the center) retrogradely labeled medial small cell somata (+). Scale bars: A , 200 μm; B , 20 μm; C , 100 μm; D , 500 μm for reconstructions, 1 mm for the inset .

    Journal: The Journal of Neuroscience

    Article Title: Neural Substrates for Species Recognition in the Time-Coding Electrosensory Pathway of Mormyrid Electric Fish

    doi: 10.1523/JNEUROSCI.18-03-01171.1998

    Figure Lengend Snippet: Injections of biotinylated dextrans into the ELp label small cell bodies in the ELa. A , Injection site in the ELp. The injection site ( ij ) is fairly anterior. Small cell bodies are labeled in the ELa, visible as dark spots . B , Close-up of the boxed area in A showing six small cell somata. C , Section of the ELp 100 μm dorsal to the injection site in A . Fibers projecting radially to the edge of the ELp are more easily visible here. D , Summary of two experiments injecting biotinylated dextrans into the ELp. i – viii , Horizontal sections of the ELa and the ELp over a dorsal–ventral series. The approximate positions of the sections are indicated in the inset . Circles in the ELp represent the injection sites, and symbols in the ELa are the positions of retrogradely labeled small cell somata. In the first experiment, the lateral injection ( solid circle ) retrogradely labels lateral small cell somata (○). In the second experiment, the medial injection ( dashed circle with + in the center) retrogradely labeled medial small cell somata (+). Scale bars: A , 200 μm; B , 20 μm; C , 100 μm; D , 500 μm for reconstructions, 1 mm for the inset .

    Article Snippet: We used broken-tipped microelectrodes (8–12 μm), filled with M r 3000 or 10,000 biotinylated dextran amines [Molecular Probes, Eugene, OR; 7% in 0.7 m KCl and 0.03% Triton X-100 (Aldrich, St. Louis, MO)].

    Techniques: Injection, Labeling

    LAR structure, sequence alignment and pulldown analysis A: BLAST alignment of the known sequences of mouse, rat and human LAR, PTPσ, PTPδ and PTPµ. The wedge domain of each protein is aligned within the box. B: The tandem intracellular phosphatase domains of human LAR with the previously characterized wedge domain (red) 14 . C-D: Pulldown of recombinant PTPσ with biotinylated ISP or ILP. E-F : Eluted lysate following pulldown was probed with antibodies against either LAR or pan-Nogo receptors. Input is 10% lysate control.

    Journal: Nature

    Article Title: Modulation of the proteoglycan receptor PTPσ promotes recovery after spinal cord injury

    doi: 10.1038/nature13974

    Figure Lengend Snippet: LAR structure, sequence alignment and pulldown analysis A: BLAST alignment of the known sequences of mouse, rat and human LAR, PTPσ, PTPδ and PTPµ. The wedge domain of each protein is aligned within the box. B: The tandem intracellular phosphatase domains of human LAR with the previously characterized wedge domain (red) 14 . C-D: Pulldown of recombinant PTPσ with biotinylated ISP or ILP. E-F : Eluted lysate following pulldown was probed with antibodies against either LAR or pan-Nogo receptors. Input is 10% lysate control.

    Article Snippet: 10% Biotinylated Dextran Amine (BDA, Molecular Probes) was injected into 16 locations in the rat motor cortex at 12w post injury.

    Techniques: Sequencing, Recombinant

    Identification and characterization of ISP A : PTPσ structure and wedge domain (red). B : Peptide Sequences. C-F : Pulldown of human, rat and mouse PTPσ with biotinylated ISP. *Nonspecific recognition of PTPδ. G-I : CSPG gradient crossing assay. Dashed Lines=CSPG gradient, scale Bar 50µm, n > 16 gradients/group. J : ISP treatment on PTPσ null neurons (n=12/group). K : Time-lapse imaging of an adult sensory neuron growth cone following 2.5µM ISP treatment ( Supplementary Video 4 ).Time-stamp=minutes. Scale bar 20µM. L : The number of neurons released from a CSPG-rich substrate following agitation (n=28 vehicle/ILP, 16 ISP wells/group). Scale bar 50µm. Error bars=SEM, One way ANOVA, Tukey’s post hoc test, *p

    Journal: Nature

    Article Title: Modulation of the proteoglycan receptor PTPσ promotes recovery after spinal cord injury

    doi: 10.1038/nature13974

    Figure Lengend Snippet: Identification and characterization of ISP A : PTPσ structure and wedge domain (red). B : Peptide Sequences. C-F : Pulldown of human, rat and mouse PTPσ with biotinylated ISP. *Nonspecific recognition of PTPδ. G-I : CSPG gradient crossing assay. Dashed Lines=CSPG gradient, scale Bar 50µm, n > 16 gradients/group. J : ISP treatment on PTPσ null neurons (n=12/group). K : Time-lapse imaging of an adult sensory neuron growth cone following 2.5µM ISP treatment ( Supplementary Video 4 ).Time-stamp=minutes. Scale bar 20µM. L : The number of neurons released from a CSPG-rich substrate following agitation (n=28 vehicle/ILP, 16 ISP wells/group). Scale bar 50µm. Error bars=SEM, One way ANOVA, Tukey’s post hoc test, *p

    Article Snippet: 10% Biotinylated Dextran Amine (BDA, Molecular Probes) was injected into 16 locations in the rat motor cortex at 12w post injury.

    Techniques: Imaging