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Boehringer Mannheim biotinylated concanavalin a
Subcellular localization of Gas3/PMP22-KK or RR-Gas3/PMP22 in NIH3T3 fibroblasts. Fifteen hours after microinjection, cells were fixed and double stained to visualize VSV and ER, using <t>biotinylated</t> <t>concanavalin</t> A (ConA). Bar = 15 μm.
Biotinylated Concanavalin A, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotinylated concanavalin a/product/Boehringer Mannheim
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
biotinylated concanavalin a - by Bioz Stars, 2021-04
86/100 stars

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1) Product Images from "Exposure at the Cell Surface Is Required for Gas3/PMP22 To Regulate Both Cell Death and Cell Spreading: Implication for the Charcot-Marie-Tooth Type 1A and Dejerine-Sottas Diseases"

Article Title: Exposure at the Cell Surface Is Required for Gas3/PMP22 To Regulate Both Cell Death and Cell Spreading: Implication for the Charcot-Marie-Tooth Type 1A and Dejerine-Sottas Diseases

Journal: Molecular Biology of the Cell

doi:

Subcellular localization of Gas3/PMP22-KK or RR-Gas3/PMP22 in NIH3T3 fibroblasts. Fifteen hours after microinjection, cells were fixed and double stained to visualize VSV and ER, using biotinylated concanavalin A (ConA). Bar = 15 μm.
Figure Legend Snippet: Subcellular localization of Gas3/PMP22-KK or RR-Gas3/PMP22 in NIH3T3 fibroblasts. Fifteen hours after microinjection, cells were fixed and double stained to visualize VSV and ER, using biotinylated concanavalin A (ConA). Bar = 15 μm.

Techniques Used: Staining

Subcellular localization of Gas3/PMP22-L16P-AAA. (a) Carboxyl-terminal sequence of Gas3/PMP22-WT showing the motif RKR mutated to AAA. (b) Subcellular localization of Gas3/PMP22-L16P-AAA in NIH3T3 fibroblasts . Gas3/PMP22-L16P, Gas3/PMP22-L16P-AAA , or Gas3/PMP22-WT-AAA were overexpressed in NIH3T3 cells by nuclear microinjection. Eighteen hours after microinjection, cells were fixed and double stained to visualize VSV and ER, using biotinylated concanavalin A. Bar = 25 μm. (c) Immunodetections were performed using antibodies against VSV on lysates from COS-7 cells transfected with the indicated Gas3/PMP22-VSV constructs. Cellular extracts were treated with or without PNGase-F and Endo-H as indicated.
Figure Legend Snippet: Subcellular localization of Gas3/PMP22-L16P-AAA. (a) Carboxyl-terminal sequence of Gas3/PMP22-WT showing the motif RKR mutated to AAA. (b) Subcellular localization of Gas3/PMP22-L16P-AAA in NIH3T3 fibroblasts . Gas3/PMP22-L16P, Gas3/PMP22-L16P-AAA , or Gas3/PMP22-WT-AAA were overexpressed in NIH3T3 cells by nuclear microinjection. Eighteen hours after microinjection, cells were fixed and double stained to visualize VSV and ER, using biotinylated concanavalin A. Bar = 25 μm. (c) Immunodetections were performed using antibodies against VSV on lysates from COS-7 cells transfected with the indicated Gas3/PMP22-VSV constructs. Cellular extracts were treated with or without PNGase-F and Endo-H as indicated.

Techniques Used: Sequencing, Staining, Transfection, Construct

2) Product Images from "Exposure at the Cell Surface Is Required for Gas3/PMP22 To Regulate Both Cell Death and Cell Spreading: Implication for the Charcot-Marie-Tooth Type 1A and Dejerine-Sottas Diseases"

Article Title: Exposure at the Cell Surface Is Required for Gas3/PMP22 To Regulate Both Cell Death and Cell Spreading: Implication for the Charcot-Marie-Tooth Type 1A and Dejerine-Sottas Diseases

Journal: Molecular Biology of the Cell

doi:

Subcellular localization of Gas3/PMP22-KK or RR-Gas3/PMP22 in NIH3T3 fibroblasts. Fifteen hours after microinjection, cells were fixed and double stained to visualize VSV and ER, using biotinylated concanavalin A (ConA). Bar = 15 μm.
Figure Legend Snippet: Subcellular localization of Gas3/PMP22-KK or RR-Gas3/PMP22 in NIH3T3 fibroblasts. Fifteen hours after microinjection, cells were fixed and double stained to visualize VSV and ER, using biotinylated concanavalin A (ConA). Bar = 15 μm.

Techniques Used: Staining

Subcellular localization of Gas3/PMP22-L16P-AAA. (a) Carboxyl-terminal sequence of Gas3/PMP22-WT showing the motif RKR mutated to AAA. (b) Subcellular localization of Gas3/PMP22-L16P-AAA in NIH3T3 fibroblasts . Gas3/PMP22-L16P, Gas3/PMP22-L16P-AAA , or Gas3/PMP22-WT-AAA were overexpressed in NIH3T3 cells by nuclear microinjection. Eighteen hours after microinjection, cells were fixed and double stained to visualize VSV and ER, using biotinylated concanavalin A. Bar = 25 μm. (c) Immunodetections were performed using antibodies against VSV on lysates from COS-7 cells transfected with the indicated Gas3/PMP22-VSV constructs. Cellular extracts were treated with or without PNGase-F and Endo-H as indicated.
Figure Legend Snippet: Subcellular localization of Gas3/PMP22-L16P-AAA. (a) Carboxyl-terminal sequence of Gas3/PMP22-WT showing the motif RKR mutated to AAA. (b) Subcellular localization of Gas3/PMP22-L16P-AAA in NIH3T3 fibroblasts . Gas3/PMP22-L16P, Gas3/PMP22-L16P-AAA , or Gas3/PMP22-WT-AAA were overexpressed in NIH3T3 cells by nuclear microinjection. Eighteen hours after microinjection, cells were fixed and double stained to visualize VSV and ER, using biotinylated concanavalin A. Bar = 25 μm. (c) Immunodetections were performed using antibodies against VSV on lysates from COS-7 cells transfected with the indicated Gas3/PMP22-VSV constructs. Cellular extracts were treated with or without PNGase-F and Endo-H as indicated.

Techniques Used: Sequencing, Staining, Transfection, Construct

Related Articles

other:

Article Title: Exposure at the Cell Surface Is Required for Gas3/PMP22 To Regulate Both Cell Death and Cell Spreading: Implication for the Charcot-Marie-Tooth Type 1A and Dejerine-Sottas Diseases
Article Snippet: Double-immunofluorescence analysis using biotinylated concanavalin A, a lectin that is specific for mannose residues, which are enriched in the ER compartment, and the anti-VSV antibody was performed as described in the Materials and Methods.

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    Boehringer Mannheim biotinylated concanavalin a
    Subcellular localization of Gas3/PMP22-KK or RR-Gas3/PMP22 in NIH3T3 fibroblasts. Fifteen hours after microinjection, cells were fixed and double stained to visualize VSV and ER, using <t>biotinylated</t> <t>concanavalin</t> A (ConA). Bar = 15 μm.
    Biotinylated Concanavalin A, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated concanavalin a/product/Boehringer Mannheim
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated concanavalin a - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

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    Subcellular localization of Gas3/PMP22-KK or RR-Gas3/PMP22 in NIH3T3 fibroblasts. Fifteen hours after microinjection, cells were fixed and double stained to visualize VSV and ER, using biotinylated concanavalin A (ConA). Bar = 15 μm.

    Journal: Molecular Biology of the Cell

    Article Title: Exposure at the Cell Surface Is Required for Gas3/PMP22 To Regulate Both Cell Death and Cell Spreading: Implication for the Charcot-Marie-Tooth Type 1A and Dejerine-Sottas Diseases

    doi:

    Figure Lengend Snippet: Subcellular localization of Gas3/PMP22-KK or RR-Gas3/PMP22 in NIH3T3 fibroblasts. Fifteen hours after microinjection, cells were fixed and double stained to visualize VSV and ER, using biotinylated concanavalin A (ConA). Bar = 15 μm.

    Article Snippet: The coverslips were treated with different first antibodies, such as anti-FLAG (Sigma, St. Louis, MO), anti-vesicular stomatitis virus (VSV) (Sigma), and anti-human placental alkaline phosphatase (hPLAP), or with biotinylated concanavalin A (Boehringer, Mannheim, Germany) diluted in PBS, and with 3% BSA for 1 h in a moist chamber at 37°C.

    Techniques: Staining

    Subcellular localization of Gas3/PMP22-L16P-AAA. (a) Carboxyl-terminal sequence of Gas3/PMP22-WT showing the motif RKR mutated to AAA. (b) Subcellular localization of Gas3/PMP22-L16P-AAA in NIH3T3 fibroblasts . Gas3/PMP22-L16P, Gas3/PMP22-L16P-AAA , or Gas3/PMP22-WT-AAA were overexpressed in NIH3T3 cells by nuclear microinjection. Eighteen hours after microinjection, cells were fixed and double stained to visualize VSV and ER, using biotinylated concanavalin A. Bar = 25 μm. (c) Immunodetections were performed using antibodies against VSV on lysates from COS-7 cells transfected with the indicated Gas3/PMP22-VSV constructs. Cellular extracts were treated with or without PNGase-F and Endo-H as indicated.

    Journal: Molecular Biology of the Cell

    Article Title: Exposure at the Cell Surface Is Required for Gas3/PMP22 To Regulate Both Cell Death and Cell Spreading: Implication for the Charcot-Marie-Tooth Type 1A and Dejerine-Sottas Diseases

    doi:

    Figure Lengend Snippet: Subcellular localization of Gas3/PMP22-L16P-AAA. (a) Carboxyl-terminal sequence of Gas3/PMP22-WT showing the motif RKR mutated to AAA. (b) Subcellular localization of Gas3/PMP22-L16P-AAA in NIH3T3 fibroblasts . Gas3/PMP22-L16P, Gas3/PMP22-L16P-AAA , or Gas3/PMP22-WT-AAA were overexpressed in NIH3T3 cells by nuclear microinjection. Eighteen hours after microinjection, cells were fixed and double stained to visualize VSV and ER, using biotinylated concanavalin A. Bar = 25 μm. (c) Immunodetections were performed using antibodies against VSV on lysates from COS-7 cells transfected with the indicated Gas3/PMP22-VSV constructs. Cellular extracts were treated with or without PNGase-F and Endo-H as indicated.

    Article Snippet: The coverslips were treated with different first antibodies, such as anti-FLAG (Sigma, St. Louis, MO), anti-vesicular stomatitis virus (VSV) (Sigma), and anti-human placental alkaline phosphatase (hPLAP), or with biotinylated concanavalin A (Boehringer, Mannheim, Germany) diluted in PBS, and with 3% BSA for 1 h in a moist chamber at 37°C.

    Techniques: Sequencing, Staining, Transfection, Construct