biotinylated antibody  (Vector Laboratories)


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    Name:
    Rabbit IgG Control Antibody
    Description:
    Rabbit IgG Control Antibody This IgG preparation is intended for use as a control for primary antibodies made in rabbit Supplied as a lyophilized powder this antibody has been purified from pooled serum of healthy adult animals and contains a spectrum of the IgG subclasses present in serum The control antibody should be applied to the tissue section at the same concentration as the primary antibody to indicate whether staining is specific for the antigen or is nonspecific adsorption of primary antibody to tissue sites
    Catalog Number:
    i-1000
    Price:
    None
    Category:
    Antibodies
    Size:
    5 mg
    Host:
    Rabbit
    Buy from Supplier


    Structured Review

    Vector Laboratories biotinylated antibody
    Rabbit IgG Control Antibody
    Rabbit IgG Control Antibody This IgG preparation is intended for use as a control for primary antibodies made in rabbit Supplied as a lyophilized powder this antibody has been purified from pooled serum of healthy adult animals and contains a spectrum of the IgG subclasses present in serum The control antibody should be applied to the tissue section at the same concentration as the primary antibody to indicate whether staining is specific for the antigen or is nonspecific adsorption of primary antibody to tissue sites
    https://www.bioz.com/result/biotinylated antibody/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated antibody - by Bioz Stars, 2021-03
    86/100 stars

    Images

    1) Product Images from "Preserved Calretinin Interneurons in an App Model of Alzheimer’s Disease Disrupt Hippocampal Inhibition via Upregulated P2Y1 Purinoreceptors"

    Article Title: Preserved Calretinin Interneurons in an App Model of Alzheimer’s Disease Disrupt Hippocampal Inhibition via Upregulated P2Y1 Purinoreceptors

    Journal: Cerebral Cortex (New York, NY)

    doi: 10.1093/cercor/bhz165

    Age-dependent phenotypical changes in the App NL-F/NL-F model of AD. ( A , C , E ) Z -stack images from confocal microscopy illustrating the expression of GFAP (for reactive astrocytes), CD68 (for microglia), and Aβ (all in red, secondary antibody Texas Red) together with DAPI staining for nuclei (in blue) in 12-month age-matched wild-type and App NL-F/NL-F mice, respectively. Similarly, bright-field images of tissue immunostained with biotinylated antibodies show conglomerates of GFAP, CD68, and Aβ in the same animals. Both immunofluorescence and immunoperoxidase-stained images taken at ×20 magnification (larger images, scale bar = 50 μm) and ×63 magnification (inserts, scale bar = 20 μm). ( B , D , F ) Analysis of GFAP, CD68, and Aβ from immunoperoxidase-stained tissue. Significant differences in the three markers of AD were seen between wild-type and App NL-F/NL-F mice only at 9–18 months and when comparing quantification at 9–18 months with the other two age cohorts. ( G , I ) Age-dependent accumulation of Aβ in selective subtypes of interneurons in hippocampal CA1. Aβ colocalization was found at significantly higher levels in SST and CCK cells (indicated by arrows), but not in calretinin (CR) cells in the same animals at 12 months (scale = 20 μm). ( J ) Quantification of colocalization of Aβ with either CCK, SST, or calretinin cells. A two-way ANOVA was performed with pairwise comparisons corrected for multiple comparisons (α = 0.05), with either post hoc Sidak’s test or Tukey’s test for multiple comparisons. * P
    Figure Legend Snippet: Age-dependent phenotypical changes in the App NL-F/NL-F model of AD. ( A , C , E ) Z -stack images from confocal microscopy illustrating the expression of GFAP (for reactive astrocytes), CD68 (for microglia), and Aβ (all in red, secondary antibody Texas Red) together with DAPI staining for nuclei (in blue) in 12-month age-matched wild-type and App NL-F/NL-F mice, respectively. Similarly, bright-field images of tissue immunostained with biotinylated antibodies show conglomerates of GFAP, CD68, and Aβ in the same animals. Both immunofluorescence and immunoperoxidase-stained images taken at ×20 magnification (larger images, scale bar = 50 μm) and ×63 magnification (inserts, scale bar = 20 μm). ( B , D , F ) Analysis of GFAP, CD68, and Aβ from immunoperoxidase-stained tissue. Significant differences in the three markers of AD were seen between wild-type and App NL-F/NL-F mice only at 9–18 months and when comparing quantification at 9–18 months with the other two age cohorts. ( G , I ) Age-dependent accumulation of Aβ in selective subtypes of interneurons in hippocampal CA1. Aβ colocalization was found at significantly higher levels in SST and CCK cells (indicated by arrows), but not in calretinin (CR) cells in the same animals at 12 months (scale = 20 μm). ( J ) Quantification of colocalization of Aβ with either CCK, SST, or calretinin cells. A two-way ANOVA was performed with pairwise comparisons corrected for multiple comparisons (α = 0.05), with either post hoc Sidak’s test or Tukey’s test for multiple comparisons. * P

    Techniques Used: Confocal Microscopy, Expressing, Staining, Mouse Assay, Immunofluorescence

    2) Product Images from "Selective Uptake of Small RNA Molecules in the Virion of Murine Gammaherpesvirus 68 ▿"

    Article Title: Selective Uptake of Small RNA Molecules in the Virion of Murine Gammaherpesvirus 68 ▿

    Journal: Journal of Virology

    doi: 10.1128/JVI.02303-08

    In situ hybridization for vtRNA1 to -4. C127 cells were infected at an MOI of 5 PFU/cell for 24 h and probed with a digoxigenin-labeled RNA probe specific for vtRNA1 to -4. Detection of the digoxigenin-labeled probe was carried out using biotinylated
    Figure Legend Snippet: In situ hybridization for vtRNA1 to -4. C127 cells were infected at an MOI of 5 PFU/cell for 24 h and probed with a digoxigenin-labeled RNA probe specific for vtRNA1 to -4. Detection of the digoxigenin-labeled probe was carried out using biotinylated

    Techniques Used: In Situ Hybridization, Infection, Labeling

    Related Articles

    Incubation:

    Article Title: Newly developed TGF-?2 knock down transgenic mouse lines express TGF-?2 differently and its distribution in multiple tissues varies
    Article Snippet: A 20 μl aliquot of the samples was loaded on to each lane and electrophoresed on 12% SDS-polyacrylamide gel (SDS-PAGE) for 2.5 h at a constant voltage of 120 V. Proteins were transferred from the gel to a nitrocellulose membrane for 6.5 h at 24 V. The membrane was blocked with phosphate-buffered saline containing 0.05% Tween-20 (PBST) with 10% nonfat dry milk overnight at 4°C for 12 h, then the membrane was washed three times for 10 min each time. .. They were then rinsed with PBST and incubated with the primary antibody for TGF-β2 (1:1000, Chemican) at 4°C for 24 h. After washing 3 times for 10 min each, the membrane was incubated with a HRP-conjugated goat anti-rabbit IgG (1:5,000; Vector Laboratories, CA) for 2 h at room temperature, and washing as described above. .. The membrane was developed in ECM kit, and then pictured by Bio-Gel Imagining system equipped with Genius synaptic gene tool software.

    Article Title: Hypothermia and Pharmacological Regimens that Prevent Overexpression and Overactivity of the Extracellular Calcium-Sensing Receptor Protect Neurons against Traumatic Brain Injury
    Article Snippet: Brains were dissected, post-fixed in 4% PFA, incubated in 20% sucrose at 4°C for 48 h, frozen, and cryosectioned (10 μm in thickness). .. Brain sections were incubated sequentially with 0.1% hydrogen peroxidase (3 min), a blocking buffer (0.5% Triton X-100, 0.1% BSA, 1.5% normal horse serum in PBS) for 30 min, and custom-made rabbit anti-CaSR (1:100) or guinea pig anti-GABA-B-R1 (1:1000) antibodies overnight at 4°C., Immunoreactivity was amplified and detected with biotinylated anti-rabbit immunoglobulin G (IgG) (1:200; Vector Laboratories, CA) or anti-guinea pig IgG (1: 500; Sigma, MO), peroxidase-conjugated avidin (ABC Elite, Vector Laboratories, CA) and diaminobenzidine substrate. .. Sections were counterstained with hematoxylin (Sigma, MO).

    Article Title: Activation of Interferon Signaling Pathways in Spinal Cord Astrocytes from an ALS Mouse Model
    Article Snippet: Transverse cryostat sections (12 μm) were processed for immunohistochemistry as described previously ( ). .. In brief, sections were incubated with primary anti-Isg15 (1:1000), anti-phospho-STAT1 (Tyr701) (1:1000), or anti-phopho-STAT2 (Tyr689) (1:1000) antibodies, followed by incubation with a biotinylated secondary antibody (Vector Laboratories). .. The detection of immunosignals was performed using a Vectastain Elite ABC kit (Vector Laboratories) and visualized with a DAB substrate solution (Roche Applied Science).

    Article Title: The Anticancer Effect of (1S,2S,3E,7E,11E)-3,7,11,15-Cembratetraen-17,2-olide(LS-1) through the Activation of TGF-β Signaling in SNU-C5/5-FU, Fluorouracil-Resistant Human Colon Cancer Cells
    Article Snippet: .. The cell lysates were separated by 6%~15% SDS-PAGE gels and then transferred to polyvinylidene fluoride membrane (BIO-RAD, Hercules, CA, USA) by glycine transfer buffer (192 mM glycine, 25 mM Tris-HCl (pH 8.8) and 20% MeOH (v/v)) at 200 mA for 2 h. After blocking with 5% skim milk solution, the membrane was incubated with primary antibody against PARP (1:2000), cleaved caspase-3 (1:1000), cleaved caspase-9 (1:1000), Bcl-2 (1:1000), Bax (1:1000), CEA (1:1000), Smad-2/3 (1:1000), p-Smad-3 (1:1000), TGFβRI (1:1000), c-Myc (1:1000), cytochrome c (1:2000) and β-actin (1:5000) antibodies at 4 °C, overnight, and incubated with a secondary HRP antibody (1:5000; Vector Laboratories, Burlingame, VT, USA) at room temperature for 1 h. Protein bands were detected using a WEST-ZOL® plus Western Blot Detection System (iNtRON, Gyeonggi, Korea) with subsequent exposure to X-ray films (AGFA, Krotich, Belgium). .. Co-Immunoprecipitation Assay SNU-C5/5-FU cells were seeded 2 × 105 cells/mL for 24 h and treated with LS-1 (7.1 μM) for 12, 24 and 48 h. After treatment, SNU-C5/5-FU cells were lysed with lysis buffer for 30 min at 4 °C.

    Article Title: Transient Disruption of Intercellular Junctions Enables Baculovirus Entry into Nondividing Hepatocytes
    Article Snippet: Endogenous biotin was blocked using the Dako Biotin Blocking System according to the manufacturer's instructions. .. Cultures were incubated in primary anti-Ac M NPV antibody or control rabbit immunoglobulin G (IgG) antibody (Vector Laboratories, Inc., Burlingame, Calif.) for 30 min, followed by three washes in PBS. ..

    Article Title: The Myc Target Gene JPO1/CDCA7 is Frequently Over-expressed in Human Tumors and has Limited Transforming Activity In Vivo
    Article Snippet: The slides were boiled in antigen unmasking solution (Vector Laboratories, Burlingame, CA) for 10 minutes, and left in the solution at room temperature for an additional 20 minutes. .. Following two more PBS washes, slides were quenched in 0.6% hydrogen peroxide/methanol, washed again in PBS and then blocked overnight in a humidifying chamber at 4° C using normal blocking serum. (Vector Laboratories, VectaStain Elite ABC kit) After washing in PBS three times, slides were incubated with either primary antibodies to JPO1 ( ) or rabbit IgG (Vector Labs) for at least one hour. .. Following another three PBS washes, slides were incubated with 2° antibody for 30 min – 1 hr and then detected with the VectaStain Elite ABC reagents and counterstained with haemotoxylin (Vector Laboratories).

    Article Title: Nuclear Interleukin-33 Is Generally Expressed in Resting Endothelium but Rapidly Lost upon Angiogenic or Proinflammatory Activation
    Article Snippet: The protein was blotted to a nitrocellulose membrane (Hybond-ECL, RPN303D; Amersham Biosciences). .. After blocking in PBS, Tween 0.05%, and 5% milk, IL-33 protein was detected by sequential incubation of blots with mouse anti-human IL-33 (Nessy-1, 1:1000), biotinylated horse anti-mouse IgG (BA-2000, 3 μg/ml; Vector Laboratories) and horseradish peroxidase-conjugated streptavidin (21124, 0.04 μg/ml; Pierce, Cramlington, UK). .. After IL-33 detection, the blots were reincubated with mouse anti-human actin (sc-8432, 1:500; Santa Cruz, Heidelberg, Germany) followed by the above-described secondary and detection methods.

    Blocking Assay:

    Article Title: Hypothermia and Pharmacological Regimens that Prevent Overexpression and Overactivity of the Extracellular Calcium-Sensing Receptor Protect Neurons against Traumatic Brain Injury
    Article Snippet: Brains were dissected, post-fixed in 4% PFA, incubated in 20% sucrose at 4°C for 48 h, frozen, and cryosectioned (10 μm in thickness). .. Brain sections were incubated sequentially with 0.1% hydrogen peroxidase (3 min), a blocking buffer (0.5% Triton X-100, 0.1% BSA, 1.5% normal horse serum in PBS) for 30 min, and custom-made rabbit anti-CaSR (1:100) or guinea pig anti-GABA-B-R1 (1:1000) antibodies overnight at 4°C., Immunoreactivity was amplified and detected with biotinylated anti-rabbit immunoglobulin G (IgG) (1:200; Vector Laboratories, CA) or anti-guinea pig IgG (1: 500; Sigma, MO), peroxidase-conjugated avidin (ABC Elite, Vector Laboratories, CA) and diaminobenzidine substrate. .. Sections were counterstained with hematoxylin (Sigma, MO).

    Article Title: The Anticancer Effect of (1S,2S,3E,7E,11E)-3,7,11,15-Cembratetraen-17,2-olide(LS-1) through the Activation of TGF-β Signaling in SNU-C5/5-FU, Fluorouracil-Resistant Human Colon Cancer Cells
    Article Snippet: .. The cell lysates were separated by 6%~15% SDS-PAGE gels and then transferred to polyvinylidene fluoride membrane (BIO-RAD, Hercules, CA, USA) by glycine transfer buffer (192 mM glycine, 25 mM Tris-HCl (pH 8.8) and 20% MeOH (v/v)) at 200 mA for 2 h. After blocking with 5% skim milk solution, the membrane was incubated with primary antibody against PARP (1:2000), cleaved caspase-3 (1:1000), cleaved caspase-9 (1:1000), Bcl-2 (1:1000), Bax (1:1000), CEA (1:1000), Smad-2/3 (1:1000), p-Smad-3 (1:1000), TGFβRI (1:1000), c-Myc (1:1000), cytochrome c (1:2000) and β-actin (1:5000) antibodies at 4 °C, overnight, and incubated with a secondary HRP antibody (1:5000; Vector Laboratories, Burlingame, VT, USA) at room temperature for 1 h. Protein bands were detected using a WEST-ZOL® plus Western Blot Detection System (iNtRON, Gyeonggi, Korea) with subsequent exposure to X-ray films (AGFA, Krotich, Belgium). .. Co-Immunoprecipitation Assay SNU-C5/5-FU cells were seeded 2 × 105 cells/mL for 24 h and treated with LS-1 (7.1 μM) for 12, 24 and 48 h. After treatment, SNU-C5/5-FU cells were lysed with lysis buffer for 30 min at 4 °C.

    Article Title: The Myc Target Gene JPO1/CDCA7 is Frequently Over-expressed in Human Tumors and has Limited Transforming Activity In Vivo
    Article Snippet: The slides were boiled in antigen unmasking solution (Vector Laboratories, Burlingame, CA) for 10 minutes, and left in the solution at room temperature for an additional 20 minutes. .. Following two more PBS washes, slides were quenched in 0.6% hydrogen peroxide/methanol, washed again in PBS and then blocked overnight in a humidifying chamber at 4° C using normal blocking serum. (Vector Laboratories, VectaStain Elite ABC kit) After washing in PBS three times, slides were incubated with either primary antibodies to JPO1 ( ) or rabbit IgG (Vector Labs) for at least one hour. .. Following another three PBS washes, slides were incubated with 2° antibody for 30 min – 1 hr and then detected with the VectaStain Elite ABC reagents and counterstained with haemotoxylin (Vector Laboratories).

    Article Title: Nuclear Interleukin-33 Is Generally Expressed in Resting Endothelium but Rapidly Lost upon Angiogenic or Proinflammatory Activation
    Article Snippet: The protein was blotted to a nitrocellulose membrane (Hybond-ECL, RPN303D; Amersham Biosciences). .. After blocking in PBS, Tween 0.05%, and 5% milk, IL-33 protein was detected by sequential incubation of blots with mouse anti-human IL-33 (Nessy-1, 1:1000), biotinylated horse anti-mouse IgG (BA-2000, 3 μg/ml; Vector Laboratories) and horseradish peroxidase-conjugated streptavidin (21124, 0.04 μg/ml; Pierce, Cramlington, UK). .. After IL-33 detection, the blots were reincubated with mouse anti-human actin (sc-8432, 1:500; Santa Cruz, Heidelberg, Germany) followed by the above-described secondary and detection methods.

    Amplification:

    Article Title: Hypothermia and Pharmacological Regimens that Prevent Overexpression and Overactivity of the Extracellular Calcium-Sensing Receptor Protect Neurons against Traumatic Brain Injury
    Article Snippet: Brains were dissected, post-fixed in 4% PFA, incubated in 20% sucrose at 4°C for 48 h, frozen, and cryosectioned (10 μm in thickness). .. Brain sections were incubated sequentially with 0.1% hydrogen peroxidase (3 min), a blocking buffer (0.5% Triton X-100, 0.1% BSA, 1.5% normal horse serum in PBS) for 30 min, and custom-made rabbit anti-CaSR (1:100) or guinea pig anti-GABA-B-R1 (1:1000) antibodies overnight at 4°C., Immunoreactivity was amplified and detected with biotinylated anti-rabbit immunoglobulin G (IgG) (1:200; Vector Laboratories, CA) or anti-guinea pig IgG (1: 500; Sigma, MO), peroxidase-conjugated avidin (ABC Elite, Vector Laboratories, CA) and diaminobenzidine substrate. .. Sections were counterstained with hematoxylin (Sigma, MO).

    Avidin-Biotin Assay:

    Article Title: Hypothermia and Pharmacological Regimens that Prevent Overexpression and Overactivity of the Extracellular Calcium-Sensing Receptor Protect Neurons against Traumatic Brain Injury
    Article Snippet: Brains were dissected, post-fixed in 4% PFA, incubated in 20% sucrose at 4°C for 48 h, frozen, and cryosectioned (10 μm in thickness). .. Brain sections were incubated sequentially with 0.1% hydrogen peroxidase (3 min), a blocking buffer (0.5% Triton X-100, 0.1% BSA, 1.5% normal horse serum in PBS) for 30 min, and custom-made rabbit anti-CaSR (1:100) or guinea pig anti-GABA-B-R1 (1:1000) antibodies overnight at 4°C., Immunoreactivity was amplified and detected with biotinylated anti-rabbit immunoglobulin G (IgG) (1:200; Vector Laboratories, CA) or anti-guinea pig IgG (1: 500; Sigma, MO), peroxidase-conjugated avidin (ABC Elite, Vector Laboratories, CA) and diaminobenzidine substrate. .. Sections were counterstained with hematoxylin (Sigma, MO).

    SDS Page:

    Article Title: The Anticancer Effect of (1S,2S,3E,7E,11E)-3,7,11,15-Cembratetraen-17,2-olide(LS-1) through the Activation of TGF-β Signaling in SNU-C5/5-FU, Fluorouracil-Resistant Human Colon Cancer Cells
    Article Snippet: .. The cell lysates were separated by 6%~15% SDS-PAGE gels and then transferred to polyvinylidene fluoride membrane (BIO-RAD, Hercules, CA, USA) by glycine transfer buffer (192 mM glycine, 25 mM Tris-HCl (pH 8.8) and 20% MeOH (v/v)) at 200 mA for 2 h. After blocking with 5% skim milk solution, the membrane was incubated with primary antibody against PARP (1:2000), cleaved caspase-3 (1:1000), cleaved caspase-9 (1:1000), Bcl-2 (1:1000), Bax (1:1000), CEA (1:1000), Smad-2/3 (1:1000), p-Smad-3 (1:1000), TGFβRI (1:1000), c-Myc (1:1000), cytochrome c (1:2000) and β-actin (1:5000) antibodies at 4 °C, overnight, and incubated with a secondary HRP antibody (1:5000; Vector Laboratories, Burlingame, VT, USA) at room temperature for 1 h. Protein bands were detected using a WEST-ZOL® plus Western Blot Detection System (iNtRON, Gyeonggi, Korea) with subsequent exposure to X-ray films (AGFA, Krotich, Belgium). .. Co-Immunoprecipitation Assay SNU-C5/5-FU cells were seeded 2 × 105 cells/mL for 24 h and treated with LS-1 (7.1 μM) for 12, 24 and 48 h. After treatment, SNU-C5/5-FU cells were lysed with lysis buffer for 30 min at 4 °C.

    Western Blot:

    Article Title: The Anticancer Effect of (1S,2S,3E,7E,11E)-3,7,11,15-Cembratetraen-17,2-olide(LS-1) through the Activation of TGF-β Signaling in SNU-C5/5-FU, Fluorouracil-Resistant Human Colon Cancer Cells
    Article Snippet: .. The cell lysates were separated by 6%~15% SDS-PAGE gels and then transferred to polyvinylidene fluoride membrane (BIO-RAD, Hercules, CA, USA) by glycine transfer buffer (192 mM glycine, 25 mM Tris-HCl (pH 8.8) and 20% MeOH (v/v)) at 200 mA for 2 h. After blocking with 5% skim milk solution, the membrane was incubated with primary antibody against PARP (1:2000), cleaved caspase-3 (1:1000), cleaved caspase-9 (1:1000), Bcl-2 (1:1000), Bax (1:1000), CEA (1:1000), Smad-2/3 (1:1000), p-Smad-3 (1:1000), TGFβRI (1:1000), c-Myc (1:1000), cytochrome c (1:2000) and β-actin (1:5000) antibodies at 4 °C, overnight, and incubated with a secondary HRP antibody (1:5000; Vector Laboratories, Burlingame, VT, USA) at room temperature for 1 h. Protein bands were detected using a WEST-ZOL® plus Western Blot Detection System (iNtRON, Gyeonggi, Korea) with subsequent exposure to X-ray films (AGFA, Krotich, Belgium). .. Co-Immunoprecipitation Assay SNU-C5/5-FU cells were seeded 2 × 105 cells/mL for 24 h and treated with LS-1 (7.1 μM) for 12, 24 and 48 h. After treatment, SNU-C5/5-FU cells were lysed with lysis buffer for 30 min at 4 °C.

    Next-Generation Sequencing:

    Article Title: Prohibitin (PHB) interacts with AKT in mitochondria to coordinately modulate sperm motility
    Article Snippet: MitoTracker mitochondria Red CMXRos probe (M7512) was purchased from Molecular Probes Inc. (Eugene, OR, USA). .. Normal goat serum (NGS), mouse immunoglobulin G (IgG), rabbit IgG, and VECTASHIELD Mounting Medium with 4',6-diamidino-2-phenylindole (DAPI; H1200) were from Vector Laboratories, Inc. (Burlingame, CA, USA). .. The PI3K inhibitor (wortmannin; #9951) was from Cell Signaling Technology, Inc. (Danvers, MA, USA).

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    Vector Laboratories goat anti mouse α tran
    Western blot findings of <t>α-tran</t> and α-gust in the small intestine of CTR, T1 and T2 animals. The values are shown with different superscripts (A, B) for p
    Goat Anti Mouse α Tran, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse α tran/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    99
    Vector Laboratories biotinylated antirat igg
    TECs exhibit different cellular distributions of endothelial molecules than normal MHECs. TECs or MHECs were plated at subculture 2 onto glass coverslips precoated with either OnFN or hFN, respectively. At 2 days postconfluence, the cells were washed with DPBS + and fixed in ice-cold methanol for 5 min at -20°C. Cell surface adhesion molecules (CD31, β-catenin, ICAM-2, and VCAM-1) were stained with specific antibodies as described in Materials and Methods section and detected with <t>antirat</t> or antirabbit <t>IgG</t> conjugated to Texas Red or FITC. Fluorescence images were captured on an Axiovert inverted microscope equipped for fluorescence using a cooled CCD camera and IP Laboratories software. All images displayed were captured with a x 40 objective. These images are representative of three separate cultures of both MHECs and TECs generated from different source tissues at different times. Images of MHECs are shown in the left hand panels (a,c,e,g) and of TECs in the right hand panels (b,d,f,g) with staining for CD31 (a,b), β-catenin (c,d), ICAM-2 (e,f), and VCAM-1 (g,h). Junctional staining of CD31 and β-catenin was discontinuous in TECs, indicating the presence of fewer cell-cell junctions. In addition, VCAM-1 did not localize to cell-cell junctions in TECs. In contrast, normal MHECs showed continuous junctional staining for CD31, β-catenin, and VCAM-1. There was little difference in distribution of ICAM-2 between MHECs and TECs.
    Biotinylated Antirat Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated antirat igg/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated antirat igg - by Bioz Stars, 2021-03
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    94
    Vector Laboratories ntsr1
    <t>NTS/NTSR1</t> complex enhanced experimental tumor growth (A) Tumor growth generated by LNM35, LNM-R and LMN-F cells xenografted into nude mice. One million cells from LNM35, LNM-R, LNM-F, or a mixture of LNM-R and LNM-F (50/50) were subcutaneously injected in 24, 36, 34, or 12 nude mice, respectively. (B) Typical immunohistochemistry for NTSR1 (left) or NTS (right) for tumors generated from LNM-R (top) or LNM-F (bottom) cells. Significant differences at *** P
    Ntsr1, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Western blot findings of α-tran and α-gust in the small intestine of CTR, T1 and T2 animals. The values are shown with different superscripts (A, B) for p

    Journal: Animals : an Open Access Journal from MDPI

    Article Title: Dietary Verbascoside Influences Gut Morphology and the Expression of α-Transducin and α-Gustducin in the Small Intestine of Weaned Piglets Exposed to n-6 Polyunsaturated Fatty Acids-Induced Oxidative Stress

    doi: 10.3390/ani9010020

    Figure Lengend Snippet: Western blot findings of α-tran and α-gust in the small intestine of CTR, T1 and T2 animals. The values are shown with different superscripts (A, B) for p

    Article Snippet: De-waxed and rehydrated paraffin sections were incubated with the first-step primary antibody, 1:10 goat anti-mouse α-tran, for 24 h at 18–20 °C, then washed in Tris-buffered saline solution (TBS 0.05 M Tris/HCl, 0.15 M NaCl pH = 7.6), and subsequently treated with the avidin–biotin blocking kit solution (Vector Laboratories Inc., Burlingame, CA, USA).

    Techniques: Western Blot

    Percentage of α-tran/chromoA or ghre-IR cells. The values are shown with different superscripts (a, b) for p

    Journal: Animals : an Open Access Journal from MDPI

    Article Title: Dietary Verbascoside Influences Gut Morphology and the Expression of α-Transducin and α-Gustducin in the Small Intestine of Weaned Piglets Exposed to n-6 Polyunsaturated Fatty Acids-Induced Oxidative Stress

    doi: 10.3390/ani9010020

    Figure Lengend Snippet: Percentage of α-tran/chromoA or ghre-IR cells. The values are shown with different superscripts (a, b) for p

    Article Snippet: De-waxed and rehydrated paraffin sections were incubated with the first-step primary antibody, 1:10 goat anti-mouse α-tran, for 24 h at 18–20 °C, then washed in Tris-buffered saline solution (TBS 0.05 M Tris/HCl, 0.15 M NaCl pH = 7.6), and subsequently treated with the avidin–biotin blocking kit solution (Vector Laboratories Inc., Burlingame, CA, USA).

    Techniques:

    Immunofluorescence findings of the enteroendocrine cells of the proximal duodenum: representative images. Double staining indicates the cytoplasmic localization of chromogranin A, or ghrelin and α-tran. ( a ) CTR, the red color is α-tran; ( b ) CTR, the green color is chromogranin A; ( c ) CTR, the red color is α-tran, the green color is chromogranin A and the yellow color is colocalization. ( d ) T2, the red color is α-tran; ( e ) T2, the green is color ghrelin; ( c ) T2, the red color is α-tran, the green is color ghrelin and yellow color is colocalization.

    Journal: Animals : an Open Access Journal from MDPI

    Article Title: Dietary Verbascoside Influences Gut Morphology and the Expression of α-Transducin and α-Gustducin in the Small Intestine of Weaned Piglets Exposed to n-6 Polyunsaturated Fatty Acids-Induced Oxidative Stress

    doi: 10.3390/ani9010020

    Figure Lengend Snippet: Immunofluorescence findings of the enteroendocrine cells of the proximal duodenum: representative images. Double staining indicates the cytoplasmic localization of chromogranin A, or ghrelin and α-tran. ( a ) CTR, the red color is α-tran; ( b ) CTR, the green color is chromogranin A; ( c ) CTR, the red color is α-tran, the green color is chromogranin A and the yellow color is colocalization. ( d ) T2, the red color is α-tran; ( e ) T2, the green is color ghrelin; ( c ) T2, the red color is α-tran, the green is color ghrelin and yellow color is colocalization.

    Article Snippet: De-waxed and rehydrated paraffin sections were incubated with the first-step primary antibody, 1:10 goat anti-mouse α-tran, for 24 h at 18–20 °C, then washed in Tris-buffered saline solution (TBS 0.05 M Tris/HCl, 0.15 M NaCl pH = 7.6), and subsequently treated with the avidin–biotin blocking kit solution (Vector Laboratories Inc., Burlingame, CA, USA).

    Techniques: Immunofluorescence, Double Staining

    TECs exhibit different cellular distributions of endothelial molecules than normal MHECs. TECs or MHECs were plated at subculture 2 onto glass coverslips precoated with either OnFN or hFN, respectively. At 2 days postconfluence, the cells were washed with DPBS + and fixed in ice-cold methanol for 5 min at -20°C. Cell surface adhesion molecules (CD31, β-catenin, ICAM-2, and VCAM-1) were stained with specific antibodies as described in Materials and Methods section and detected with antirat or antirabbit IgG conjugated to Texas Red or FITC. Fluorescence images were captured on an Axiovert inverted microscope equipped for fluorescence using a cooled CCD camera and IP Laboratories software. All images displayed were captured with a x 40 objective. These images are representative of three separate cultures of both MHECs and TECs generated from different source tissues at different times. Images of MHECs are shown in the left hand panels (a,c,e,g) and of TECs in the right hand panels (b,d,f,g) with staining for CD31 (a,b), β-catenin (c,d), ICAM-2 (e,f), and VCAM-1 (g,h). Junctional staining of CD31 and β-catenin was discontinuous in TECs, indicating the presence of fewer cell-cell junctions. In addition, VCAM-1 did not localize to cell-cell junctions in TECs. In contrast, normal MHECs showed continuous junctional staining for CD31, β-catenin, and VCAM-1. There was little difference in distribution of ICAM-2 between MHECs and TECs.

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Murine Lewis Lung Carcinoma-Derived Endothelium Expresses Markers of Endothelial Activation and Requires Tumor-Specific Extracellular Matrix In Vitro 1

    doi:

    Figure Lengend Snippet: TECs exhibit different cellular distributions of endothelial molecules than normal MHECs. TECs or MHECs were plated at subculture 2 onto glass coverslips precoated with either OnFN or hFN, respectively. At 2 days postconfluence, the cells were washed with DPBS + and fixed in ice-cold methanol for 5 min at -20°C. Cell surface adhesion molecules (CD31, β-catenin, ICAM-2, and VCAM-1) were stained with specific antibodies as described in Materials and Methods section and detected with antirat or antirabbit IgG conjugated to Texas Red or FITC. Fluorescence images were captured on an Axiovert inverted microscope equipped for fluorescence using a cooled CCD camera and IP Laboratories software. All images displayed were captured with a x 40 objective. These images are representative of three separate cultures of both MHECs and TECs generated from different source tissues at different times. Images of MHECs are shown in the left hand panels (a,c,e,g) and of TECs in the right hand panels (b,d,f,g) with staining for CD31 (a,b), β-catenin (c,d), ICAM-2 (e,f), and VCAM-1 (g,h). Junctional staining of CD31 and β-catenin was discontinuous in TECs, indicating the presence of fewer cell-cell junctions. In addition, VCAM-1 did not localize to cell-cell junctions in TECs. In contrast, normal MHECs showed continuous junctional staining for CD31, β-catenin, and VCAM-1. There was little difference in distribution of ICAM-2 between MHECs and TECs.

    Article Snippet: Serial sections were stained with antibodies to CD31, CD102, CD144, CD106, Flk-1, and CD62E at 1 µg/ml, detected with biotinylated antirat IgG, and developed with the NovaRED substrate kit (Vector Laboratories).

    Techniques: Staining, Fluorescence, Inverted Microscopy, Software, Generated

    Comparison of untreated KAT-4 and Capan-2 carcinoma models. a Hematoxylin and Eosin and Sirius red staining in KAT-4 and Capan-2 carcinomas (bars = 100 μm). Trichrome staining (bars = 20 μm) and immunofluorescence staining with biotinylated 3G9 antibody (green) shows that the expression of integrin α V β 6 is located at the cell membrane in both KAT-4 and Capan-2 carcinomas (cell nuclei stained with DAPI, blue; bars = 50 μm). b Collagen content in untreated KAT-4 ( n = 4) and Capan-2 ( n = 5) carcinomas, represented by hydroxyproline mg/g wet weight. c Average growth of untreated KAT-4 ( n = 8) and Capan-2 tumors ( n = 7), represented in mm 3 measured externally (length x width x height)

    Journal: Cell Communication and Signaling : CCS

    Article Title: Inhibition of integrin αVβ6 changes fibril thickness of stromal collagen in experimental carcinomas

    doi: 10.1186/s12964-018-0249-7

    Figure Lengend Snippet: Comparison of untreated KAT-4 and Capan-2 carcinoma models. a Hematoxylin and Eosin and Sirius red staining in KAT-4 and Capan-2 carcinomas (bars = 100 μm). Trichrome staining (bars = 20 μm) and immunofluorescence staining with biotinylated 3G9 antibody (green) shows that the expression of integrin α V β 6 is located at the cell membrane in both KAT-4 and Capan-2 carcinomas (cell nuclei stained with DAPI, blue; bars = 50 μm). b Collagen content in untreated KAT-4 ( n = 4) and Capan-2 ( n = 5) carcinomas, represented by hydroxyproline mg/g wet weight. c Average growth of untreated KAT-4 ( n = 8) and Capan-2 tumors ( n = 7), represented in mm 3 measured externally (length x width x height)

    Article Snippet: Sections were blocked in 5% swine serum (Sigma) and 2% BSA (Sigma), incubated with biotinylated 3G9 antibody and then with Fluorescein Avidin D (Vector Labs).

    Techniques: Staining, Immunofluorescence, Expressing

    NTS/NTSR1 complex enhanced experimental tumor growth (A) Tumor growth generated by LNM35, LNM-R and LMN-F cells xenografted into nude mice. One million cells from LNM35, LNM-R, LNM-F, or a mixture of LNM-R and LNM-F (50/50) were subcutaneously injected in 24, 36, 34, or 12 nude mice, respectively. (B) Typical immunohistochemistry for NTSR1 (left) or NTS (right) for tumors generated from LNM-R (top) or LNM-F (bottom) cells. Significant differences at *** P

    Journal: Oncotarget

    Article Title: Neurotensin (NTS) and its receptor (NTSR1) causes EGFR, HER2 and HER3 over-expression and their autocrine/paracrine activation in lung tumors, confirming responsiveness to erlotinib

    doi:

    Figure Lengend Snippet: NTS/NTSR1 complex enhanced experimental tumor growth (A) Tumor growth generated by LNM35, LNM-R and LMN-F cells xenografted into nude mice. One million cells from LNM35, LNM-R, LNM-F, or a mixture of LNM-R and LNM-F (50/50) were subcutaneously injected in 24, 36, 34, or 12 nude mice, respectively. (B) Typical immunohistochemistry for NTSR1 (left) or NTS (right) for tumors generated from LNM-R (top) or LNM-F (bottom) cells. Significant differences at *** P

    Article Snippet: These slides were then incubated with appropriate biotinylated secondary antibodies, NTS (Trekkie Biotinylates rabbit link, Biocare medical®), NTSR1 (Biotinylated anti-goat IgG, Vector laboratories, Inc), ErbB3 (Trekkie Biotinylates mouse link, Biocare medical®).

    Techniques: Generated, Mouse Assay, Injection, Immunohistochemistry

    Immunohistochemistry of NTSR1, lung cancer tumors (A) NTSR1 Immunolabeling in patients with primary lung adenocarcinomas (right) top X 50, bottom X400) and Squamous Cell carcinomas (left) . top X100, bottom X200. (B) Overall survival of patients operated for NSCLC lung adenocarcinoma according to NTSR1 score. Semiquantitative immunohistochemistry evaluation of NTSR1: NTSR1 + + +: strongly positive expression (number of staining cells > 50% and the labeling intensity is high = score 2), other: the remaining patients (score 0 and 1). left Survival curve for the first cohort, Center Survival curve for lung adenocarcinomas from the first cohort, right Survival curve for SCC and LCC from the first cohort (C) Overall survival of patients operated for lung adenocarcinoma according to NTSR1 score. Semiquantitative immunohistochemistry evaluation of NTSR1: NTSR1 + + +: strongly positive expression (number of staining cells > 50% and the labeling intensity is high = score 2), other: the remaining patients (score 0 and 1).

    Journal: Oncotarget

    Article Title: Neurotensin (NTS) and its receptor (NTSR1) causes EGFR, HER2 and HER3 over-expression and their autocrine/paracrine activation in lung tumors, confirming responsiveness to erlotinib

    doi:

    Figure Lengend Snippet: Immunohistochemistry of NTSR1, lung cancer tumors (A) NTSR1 Immunolabeling in patients with primary lung adenocarcinomas (right) top X 50, bottom X400) and Squamous Cell carcinomas (left) . top X100, bottom X200. (B) Overall survival of patients operated for NSCLC lung adenocarcinoma according to NTSR1 score. Semiquantitative immunohistochemistry evaluation of NTSR1: NTSR1 + + +: strongly positive expression (number of staining cells > 50% and the labeling intensity is high = score 2), other: the remaining patients (score 0 and 1). left Survival curve for the first cohort, Center Survival curve for lung adenocarcinomas from the first cohort, right Survival curve for SCC and LCC from the first cohort (C) Overall survival of patients operated for lung adenocarcinoma according to NTSR1 score. Semiquantitative immunohistochemistry evaluation of NTSR1: NTSR1 + + +: strongly positive expression (number of staining cells > 50% and the labeling intensity is high = score 2), other: the remaining patients (score 0 and 1).

    Article Snippet: These slides were then incubated with appropriate biotinylated secondary antibodies, NTS (Trekkie Biotinylates rabbit link, Biocare medical®), NTSR1 (Biotinylated anti-goat IgG, Vector laboratories, Inc), ErbB3 (Trekkie Biotinylates mouse link, Biocare medical®).

    Techniques: Immunohistochemistry, Immunolabeling, Expressing, Staining, Labeling

    NTS regulation enhanced HER2, and HER3 basal expression in human lung cancer cell lines (A) The mixture of cells R/F 20/80 lung cancer cells cultured for 72h, with the histograms representing intensity-based quantification of Western blot bands of basal total protein, EGFR, HER2, and HER3, Values are expressed as the percentage of the control LNM-F cells (which are the population more representative of the mixture) and are the mean ± SEM of 5 to 8 independent experiments. (B) An example of western blot gel of LNM-F, LNM-R and the mixture LNM-F, LNM-R (20/80) cultured for 72h no treated or treated with DMSO or 5x10 -6 M SR 48692. The blots were revealed with EGFR, HER2 or HER3 antibodies. The actin shown is to the protein control for the HER3 Blot (C) Lung cancer cells R-SI NTS treated or not with 10 -7 M JMV 449, DMSO or 5x10 -6 M SR 48692 for 48h. The histograms represent intensity-based quantification of Western blot bands of basal total protein, EGFR, HER2, and HER3. Values are expressed as the percentage of the non-treated cells (control), and are the mean ± SEM of 3 to 6 independent experiments.. Inset , An example of western blot gel of R-SI NTS cells treated with 10 -7 M JMV449, DMSO or 5x10 -6 M SR 48692 for 48h. Western blot bands of basal total EGFR, HER2, and HER3 protein. (D) EGFR, HER2, and HER3 immunolabeling in R-SI NTS cells treated of not with 10 -7 M JMV449 for 48 h. (E) Example of two restrictive areas from a patient with lung adenocarcinoma with a positives labeling for NTS, NTSR1, HER2, HER3.

    Journal: Oncotarget

    Article Title: Neurotensin (NTS) and its receptor (NTSR1) causes EGFR, HER2 and HER3 over-expression and their autocrine/paracrine activation in lung tumors, confirming responsiveness to erlotinib

    doi:

    Figure Lengend Snippet: NTS regulation enhanced HER2, and HER3 basal expression in human lung cancer cell lines (A) The mixture of cells R/F 20/80 lung cancer cells cultured for 72h, with the histograms representing intensity-based quantification of Western blot bands of basal total protein, EGFR, HER2, and HER3, Values are expressed as the percentage of the control LNM-F cells (which are the population more representative of the mixture) and are the mean ± SEM of 5 to 8 independent experiments. (B) An example of western blot gel of LNM-F, LNM-R and the mixture LNM-F, LNM-R (20/80) cultured for 72h no treated or treated with DMSO or 5x10 -6 M SR 48692. The blots were revealed with EGFR, HER2 or HER3 antibodies. The actin shown is to the protein control for the HER3 Blot (C) Lung cancer cells R-SI NTS treated or not with 10 -7 M JMV 449, DMSO or 5x10 -6 M SR 48692 for 48h. The histograms represent intensity-based quantification of Western blot bands of basal total protein, EGFR, HER2, and HER3. Values are expressed as the percentage of the non-treated cells (control), and are the mean ± SEM of 3 to 6 independent experiments.. Inset , An example of western blot gel of R-SI NTS cells treated with 10 -7 M JMV449, DMSO or 5x10 -6 M SR 48692 for 48h. Western blot bands of basal total EGFR, HER2, and HER3 protein. (D) EGFR, HER2, and HER3 immunolabeling in R-SI NTS cells treated of not with 10 -7 M JMV449 for 48 h. (E) Example of two restrictive areas from a patient with lung adenocarcinoma with a positives labeling for NTS, NTSR1, HER2, HER3.

    Article Snippet: These slides were then incubated with appropriate biotinylated secondary antibodies, NTS (Trekkie Biotinylates rabbit link, Biocare medical®), NTSR1 (Biotinylated anti-goat IgG, Vector laboratories, Inc), ErbB3 (Trekkie Biotinylates mouse link, Biocare medical®).

    Techniques: Expressing, Cell Culture, Western Blot, Immunolabeling, Labeling

    NTS/NTSR1 expressing tumors are the target for EGFR inhibitors treatment (A) LNM-R or R-SI NTSR1 cells (LNM-R expressing sh-RNA for NTSR1) were injected into the left and the right flank of the mice, respectively. Here is shown an example of a mouse from each group after 17 days of treatment. ( B and C ) Tumor growth generated by LNM-R cells (left flank) and R-SI NTSR1 cells (right flank) xenografted into nude mice and treated for 17 days with water, or 25 mg/kg erlotinib, or 200 mg/kg metformin, or both. At day one, 9 mice per group were randomized on LNM-R tumors size reaching approximately 20 mm 3 . (D) Tumor growth generated by R-SI NTSR1 cells xenografted into nude mice and treated for 24 days with water, or 25 mg/kg erlotinib, or 200 mg/kg metformin, or both. At day one, 10 mice per group were randomized on tumors size reaching approximately at 150 mm 3 .

    Journal: Oncotarget

    Article Title: Neurotensin (NTS) and its receptor (NTSR1) causes EGFR, HER2 and HER3 over-expression and their autocrine/paracrine activation in lung tumors, confirming responsiveness to erlotinib

    doi:

    Figure Lengend Snippet: NTS/NTSR1 expressing tumors are the target for EGFR inhibitors treatment (A) LNM-R or R-SI NTSR1 cells (LNM-R expressing sh-RNA for NTSR1) were injected into the left and the right flank of the mice, respectively. Here is shown an example of a mouse from each group after 17 days of treatment. ( B and C ) Tumor growth generated by LNM-R cells (left flank) and R-SI NTSR1 cells (right flank) xenografted into nude mice and treated for 17 days with water, or 25 mg/kg erlotinib, or 200 mg/kg metformin, or both. At day one, 9 mice per group were randomized on LNM-R tumors size reaching approximately 20 mm 3 . (D) Tumor growth generated by R-SI NTSR1 cells xenografted into nude mice and treated for 24 days with water, or 25 mg/kg erlotinib, or 200 mg/kg metformin, or both. At day one, 10 mice per group were randomized on tumors size reaching approximately at 150 mm 3 .

    Article Snippet: These slides were then incubated with appropriate biotinylated secondary antibodies, NTS (Trekkie Biotinylates rabbit link, Biocare medical®), NTSR1 (Biotinylated anti-goat IgG, Vector laboratories, Inc), ErbB3 (Trekkie Biotinylates mouse link, Biocare medical®).

    Techniques: Expressing, Injection, Mouse Assay, Generated

    NTS autocrine and paracrine regulation enhanced cellular growth in human lung cancer cell lines (A) Influence of NTS exogenous treatment on lung cancer cell growth. LNM-F, R-SI NTS and R-SI NTSR1 were grown in media containing 0 % FCS at low concentration and treated every day with 10 -8 M NTS or JMV 449 for 6 days. The ratio of the number of cells at Day 6/Day 0 was calculated. The result is expressed as the % of fold induction. Inset , NTS and NTSR1 transcripts analysis from a total of 200 ng of LNM-35, LNM-R, LNM-F, R-SI NTSR1 and R-SI NTS total RNA. (B) LNM-R and LNM-F were seeded alone or at the ratio of 20/80 LNM-R/LNM-F and grown in 0.1% FCS for 72h. The results are expressed as the ratio of the number of cells at 72h to T0 was calculated, and are the mean ± SEM of 7 independent experiments. C to F ) LNM-R and LNM-F were seeded alone or at the ratio of 20/80 LNM-R/LNM-F and grown in 0.1% FCS for 72h, The ratio of the number of cells at 72h to T0 was calculated. The results are expressed as the percentage of the growth induction compared to LNM-F. Results are the mean ± SEM of 2 to 5 independent experiments. Cells were exposed to (C) DMSO, 10 -7 M BIM 46174, 10 -6 M SR 48692, 1/200 rabbit IgG or anti NTS antibody. (D) DMSO, 10 -7 M M475271, 10 -5 M AG1478, PBS, or 50 μg/ml Herceptin. (E) 5×10 -6 M Gö6976, 10 -6 M U0126, 10 -6 M PD98059, or 10 -7 M LY294002. F) DMSO, 10 -5 M D-NAME, 10 -5 M L-NAME, 10 -5 M H7 or 5 10 -6 M Gö6976.

    Journal: Oncotarget

    Article Title: Neurotensin (NTS) and its receptor (NTSR1) causes EGFR, HER2 and HER3 over-expression and their autocrine/paracrine activation in lung tumors, confirming responsiveness to erlotinib

    doi:

    Figure Lengend Snippet: NTS autocrine and paracrine regulation enhanced cellular growth in human lung cancer cell lines (A) Influence of NTS exogenous treatment on lung cancer cell growth. LNM-F, R-SI NTS and R-SI NTSR1 were grown in media containing 0 % FCS at low concentration and treated every day with 10 -8 M NTS or JMV 449 for 6 days. The ratio of the number of cells at Day 6/Day 0 was calculated. The result is expressed as the % of fold induction. Inset , NTS and NTSR1 transcripts analysis from a total of 200 ng of LNM-35, LNM-R, LNM-F, R-SI NTSR1 and R-SI NTS total RNA. (B) LNM-R and LNM-F were seeded alone or at the ratio of 20/80 LNM-R/LNM-F and grown in 0.1% FCS for 72h. The results are expressed as the ratio of the number of cells at 72h to T0 was calculated, and are the mean ± SEM of 7 independent experiments. C to F ) LNM-R and LNM-F were seeded alone or at the ratio of 20/80 LNM-R/LNM-F and grown in 0.1% FCS for 72h, The ratio of the number of cells at 72h to T0 was calculated. The results are expressed as the percentage of the growth induction compared to LNM-F. Results are the mean ± SEM of 2 to 5 independent experiments. Cells were exposed to (C) DMSO, 10 -7 M BIM 46174, 10 -6 M SR 48692, 1/200 rabbit IgG or anti NTS antibody. (D) DMSO, 10 -7 M M475271, 10 -5 M AG1478, PBS, or 50 μg/ml Herceptin. (E) 5×10 -6 M Gö6976, 10 -6 M U0126, 10 -6 M PD98059, or 10 -7 M LY294002. F) DMSO, 10 -5 M D-NAME, 10 -5 M L-NAME, 10 -5 M H7 or 5 10 -6 M Gö6976.

    Article Snippet: These slides were then incubated with appropriate biotinylated secondary antibodies, NTS (Trekkie Biotinylates rabbit link, Biocare medical®), NTSR1 (Biotinylated anti-goat IgG, Vector laboratories, Inc), ErbB3 (Trekkie Biotinylates mouse link, Biocare medical®).

    Techniques: Concentration Assay