biotinylated anti rabbit secondary antibody  (Vector Laboratories)


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    Name:
    Biotinylated Rabbit Anti Rat IgG Antibody
    Description:
    Biotinylated Rabbit Anti Rat IgG Antibody is prepared using proprietary immunization schedules that produce high affinity antibodies The antibodies are then purified by affinity chromatography and cross reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining ELISAs and blots Biotinylated Rabbit Anti Rat IgG H L is supplied in lyophilized form and can be reconstituted with 1 ml water With some exceptions the recommended dilution for most applications is 1 200 H L indicates the antibody recognizes both heavy and light chains This antibody is included in the VECTASTAIN ABC kits
    Catalog Number:
    ba-4000
    Price:
    None
    Category:
    Antibodies
    Reactivity:
    Rat
    Size:
    1 5 mg
    Host:
    Rabbit
    Buy from Supplier


    Structured Review

    Vector Laboratories biotinylated anti rabbit secondary antibody
    Biotinylated Rabbit Anti Rat IgG Antibody
    Biotinylated Rabbit Anti Rat IgG Antibody is prepared using proprietary immunization schedules that produce high affinity antibodies The antibodies are then purified by affinity chromatography and cross reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining ELISAs and blots Biotinylated Rabbit Anti Rat IgG H L is supplied in lyophilized form and can be reconstituted with 1 ml water With some exceptions the recommended dilution for most applications is 1 200 H L indicates the antibody recognizes both heavy and light chains This antibody is included in the VECTASTAIN ABC kits
    https://www.bioz.com/result/biotinylated anti rabbit secondary antibody/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated anti rabbit secondary antibody - by Bioz Stars, 2021-03
    86/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Immunohistochemical analysis of macroautophagy
    Article Snippet: Subsequently, the primary antibody was applied overnight in a damp box, followed by 2 × 5 min immersion in staining buffer. .. Next, the slides were incubated for 30 min with biotinylated anti-rabbit or anti-mouse immunoglobulins (Vectastain ABC Kit, Vector Laboratories), 1:200 diluted in staining buffer. .. In case the primary antibody was already biotinylated, blocking with normal serum and incubation with biotinylated secondary antibodies were omitted.

    Article Title: Human epidermal growth factor receptor 2 overexpression in breast cancer of patients with anti-Yo-associated paraneoplastic cerebellar degeneration
    Article Snippet: To determine the frequency of cdr2 expression in HER2-positive breast tumors, paraffin sections of 8 HER2-positive breast tumors from patients without PCD were deparaffinized in xylene, rehydrated in alcohol, washed in tap water, and heated for 10 min in a pressure-cooking oven in 0.1 M sodium citrate buffer (pH, 6.0). .. After inhibition of endogenous peroxidase with 0.3% hydrogen peroxide in phosphate-buffered saline (PBS) for 15 min, sections were sequentially incubated with undiluted normal human serum or a human serum with high titers of Yo antibodies for 2 hours at room temperature, followed by biotinylated anti-Yo IgG overnight at 4°C (obtained as previously described ), and the Vectastain Elite ABC complex (Vector Labs) for 30 min. ..

    Article Title: Abnormalities of Thymic Stroma may Contribute to Immune Dysregulation in Murine Models of Leaky Severe Combined Immunodeficiency
    Article Snippet: After washes, single immunostains were revealed using the ChemMATE Envision Rabbit/Mouse (DAKO Cytomation, Glostrup, Denmark) or NovoLinkTM Polymer Detection System (NovocastraTM Laboratories Ltd, Newcastle Upon Tyne, United Kingdom) followed by diaminobenzidine (DAB) as chromogen and Hematoxylin as counterstain. .. The same procedure was applied to double immunofluorescence stainings prior to the incubation with a secondary swine anti-rabbit FITC-conjugated antibody (1:30; DAKO Cytomation, Glostrup, Denmark) for CK5 and a rabbit anti-rat biotinylated antibody (1:200; Vector Laboratories, Burlingame, CA, USA) followed by Streptavidin–Texas Red (1:100; Southern Biotechnology Associates, Birmingham, AL, USA) for CK8. ..

    Staining:

    Article Title: Immunohistochemical analysis of macroautophagy
    Article Snippet: Subsequently, the primary antibody was applied overnight in a damp box, followed by 2 × 5 min immersion in staining buffer. .. Next, the slides were incubated for 30 min with biotinylated anti-rabbit or anti-mouse immunoglobulins (Vectastain ABC Kit, Vector Laboratories), 1:200 diluted in staining buffer. .. In case the primary antibody was already biotinylated, blocking with normal serum and incubation with biotinylated secondary antibodies were omitted.

    Article Title: The specific JNK inhibitor SP600125 targets tumour necrosis factor-? production and epithelial cell apoptosis in acute murine colitis
    Article Snippet: The following antibodies were used at the indicated dilutions: PpJNK (1 : 100, NEB, Pickering, CAN), anti-rat F4/80 (1 : 100). .. Sections were stained using a Vectastain ABC elite kit and biotinylated ant-rabbit (or anti-rat) antibody, and avidin D–fluorescein isothiocyanate used for immunofluorescence (Vector Laboratories, Burlingame, CA). ..

    Avidin-Biotin Assay:

    Article Title: Assembly with the NR1 Subunit Is Required for Surface Expression of NR3A-Containing NMDA Receptors
    Article Snippet: For immunostaining with rabbit anti-calreticulin (1:200) and mouse anti-Grp78 (1:200; Stressgen Biotechnologies, Victoria, British Columbia, Canada) antibodies, cells were permeabilized with 0.1% Triton X-100 in PBS for 10 min before addition of the primary antibody. .. Biotinylated anti-rabbit secondary antibody (1:1000; Vector Laboratories) followed by avidin-Texas Red (1:1000; Vector Laboratories) or Cy3-conjugated anti-mouse antibody (1:600; Jackson ImmunoResearch) was used. .. Cells were then washed and mounted, and images were acquired using a Zeiss confocal microscope.

    Article Title: Anti-α4β1 Integrin Antibodies Attenuated Brain Inflammatory Changes in a Mouse Model of Alzheimer’s Disease
    Article Snippet: All mouse cytokine ELISA kits were obtained from R & D Systems (Minneapolis, MN). .. Elite Vectastain ABC avidin and biotin kits, biotinylated anti-rabbit, anti-mouse, and anti-rat antibodies and the Vector VIP kits were from Vector Laboratories Inc. (Burlingame, CA). .. Anti-CD68 was obtained from Serotec (Raleigh, NC).

    Article Title: Trafficking to the Plasma Membrane of the Seven-Transmembrane Protein Encoded by Human Herpesvirus 6 U51 Gene Involves a Cell-Specific Function Present in T Lymphocytes
    Article Snippet: Infection of CBMCs with HHV-6 was monitored routinely by immunofluorescence with monoclonal antibody (MAb) 2D10 to glycoprotein B (gB), as described elsewhere ( ). .. Commercially available antibodies were antihemagglutinin (anti-HA) antibody (AntiXpress) (Invitrogen), anti-CC-chemokine receptor 5 (anti-CCR5) MAb LS100/2D7 (R & D Systems, Abingdon, United Kingdom), fluorescein isothiocyanate (FITC)-conjugated anti-mouse antibody (Jackson), FITC-conjugated anti-rabbit antibody (Dako), biotinylated anti-rabbit antibody and avidin-biotin-peroxidase (Vector Laboratories), and FITC-conjugated goat F(ab′)2 anti-mouse antibody (Becton Dickinson). ..

    Article Title: The specific JNK inhibitor SP600125 targets tumour necrosis factor-? production and epithelial cell apoptosis in acute murine colitis
    Article Snippet: The following antibodies were used at the indicated dilutions: PpJNK (1 : 100, NEB, Pickering, CAN), anti-rat F4/80 (1 : 100). .. Sections were stained using a Vectastain ABC elite kit and biotinylated ant-rabbit (or anti-rat) antibody, and avidin D–fluorescein isothiocyanate used for immunofluorescence (Vector Laboratories, Burlingame, CA). ..

    Inhibition:

    Article Title: Human epidermal growth factor receptor 2 overexpression in breast cancer of patients with anti-Yo-associated paraneoplastic cerebellar degeneration
    Article Snippet: To determine the frequency of cdr2 expression in HER2-positive breast tumors, paraffin sections of 8 HER2-positive breast tumors from patients without PCD were deparaffinized in xylene, rehydrated in alcohol, washed in tap water, and heated for 10 min in a pressure-cooking oven in 0.1 M sodium citrate buffer (pH, 6.0). .. After inhibition of endogenous peroxidase with 0.3% hydrogen peroxide in phosphate-buffered saline (PBS) for 15 min, sections were sequentially incubated with undiluted normal human serum or a human serum with high titers of Yo antibodies for 2 hours at room temperature, followed by biotinylated anti-Yo IgG overnight at 4°C (obtained as previously described ), and the Vectastain Elite ABC complex (Vector Labs) for 30 min. ..

    Plasmid Preparation:

    Article Title: Anti-α4β1 Integrin Antibodies Attenuated Brain Inflammatory Changes in a Mouse Model of Alzheimer’s Disease
    Article Snippet: All mouse cytokine ELISA kits were obtained from R & D Systems (Minneapolis, MN). .. Elite Vectastain ABC avidin and biotin kits, biotinylated anti-rabbit, anti-mouse, and anti-rat antibodies and the Vector VIP kits were from Vector Laboratories Inc. (Burlingame, CA). .. Anti-CD68 was obtained from Serotec (Raleigh, NC).

    Immunofluorescence:

    Article Title: Abnormalities of Thymic Stroma may Contribute to Immune Dysregulation in Murine Models of Leaky Severe Combined Immunodeficiency
    Article Snippet: After washes, single immunostains were revealed using the ChemMATE Envision Rabbit/Mouse (DAKO Cytomation, Glostrup, Denmark) or NovoLinkTM Polymer Detection System (NovocastraTM Laboratories Ltd, Newcastle Upon Tyne, United Kingdom) followed by diaminobenzidine (DAB) as chromogen and Hematoxylin as counterstain. .. The same procedure was applied to double immunofluorescence stainings prior to the incubation with a secondary swine anti-rabbit FITC-conjugated antibody (1:30; DAKO Cytomation, Glostrup, Denmark) for CK5 and a rabbit anti-rat biotinylated antibody (1:200; Vector Laboratories, Burlingame, CA, USA) followed by Streptavidin–Texas Red (1:100; Southern Biotechnology Associates, Birmingham, AL, USA) for CK8. ..

    Article Title: The specific JNK inhibitor SP600125 targets tumour necrosis factor-? production and epithelial cell apoptosis in acute murine colitis
    Article Snippet: The following antibodies were used at the indicated dilutions: PpJNK (1 : 100, NEB, Pickering, CAN), anti-rat F4/80 (1 : 100). .. Sections were stained using a Vectastain ABC elite kit and biotinylated ant-rabbit (or anti-rat) antibody, and avidin D–fluorescein isothiocyanate used for immunofluorescence (Vector Laboratories, Burlingame, CA). ..

    Labeling:

    Article Title: Endocytosis of a Glycosylphosphatidylinositol-anchored Protein via Clathrin-coated Vesicles, Sorting by Default in Endosomes, and Exocytosis via RAB11-positive Carriers V⃞
    Article Snippet: For double labeling (Figures , inset; ), the first antibody was generally detected by PAG-10 or PAG-6; after a block with a rabbit normal IgG (1:400), the second antibody was detected by PAG-10 or PAG-15. .. In one case ( , inset), clathrin was first labeled with rabbit anti-TbCLH antibodies and PAG-6, whereas VSG was subsequently detected using biotinylated rabbit anti-VSG IgG followed by a monoclonal mouse antibiotin antibody (1:100; Vector Laboratories, Burlingame, CA) and goat anti-mouse IgG-18-nm gold conjugates (1:20; Jackson Immunoresearch Laboratories, West Grove, PA). .. After labeling, the cryosections were embedded in 1.4% methylcellulose, 0.3% uranyl acetate.

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  • 97
    Vector Laboratories biotinylated rabbit anti goat secondary antibodies
    OXA- (A and B) and OX1R- (C and D) immunoreactivity in the human normal prostate. (A) Positive cells scattered along the monolayered epithelium of a prostatic follicle. (B) The majority of positive cells visible in this micrograph contain differently stained granular material in the apical portion of their cytoplasm. (C and D) Positive cells lined along the stratified epithelium of three follicles. Some of them are elongated in shape and completely filled with clusters of immunoreactive granules. <t>Avidin–biotin</t> immunohistochemical method. Scale bars: 20 μm.
    Biotinylated Rabbit Anti Goat Secondary Antibodies, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated rabbit anti goat secondary antibodies/product/Vector Laboratories
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated rabbit anti goat secondary antibodies - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

    97
    Vector Laboratories biotinylated goat anti rabbit igg
    Distribution of avian influenza virus NP in selected organs of inoculated birds. Distribution of influenza NP in brain ( A , E , I , M ), lung ( B , F , J , N ), spleen ( C , G , K , O ) and heart ( D , H , L , P ) of inoculated chickens of selected viruses at 4 dpi (except for H5N1_H4_T 327 K) as detected by immunohistochemistry using primary polyclonal rabbit anti-NP A/FPV/Rostock/34 antibody (1:750) and a secondary <t>biotinylated</t> goat anti-rabbit <t>IgG</t> (Vector Laboratories, Burlingame, CA, USA) antibody (1:200). 3-amino-9-ethyl-carbazol (red-brown); hematoxylin counterstain (blue); Nomarski contrast; bars A,B,D,E,F,H,I,J,L,M,N,P = 20 µm. Bars C,G,K,O = 50 µm.
    Biotinylated Goat Anti Rabbit Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated goat anti rabbit igg/product/Vector Laboratories
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated goat anti rabbit igg - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

    97
    Vector Laboratories biotinylated universal secondary antibody
    Representative histological images of the insulin- and glucagon-immunoreactive cells in the pancreas, taken from NFD or HFD supplied mice. ( a ) Vehicle (distilled water) 10 mL/kg orally administered mice with NFD supply (Intact control); ( b ) Vehicle 10 mL/kg orally administered mice with HFD supply (HFD control); ( c ) 250 mg/kg of metformin oral administered mice with HFD supply (Metformin); ( d ) WL 200 mg/kg orally administered mice with HFD supply (WL200); ( e ) WL 100 mg/kg orally administered mice with HFD supply (WL100); ( f ) WL 50 mg/kg orally administered mice with HFD supply (WL50). NFD = Normal pellet diet; HFD = 45% Kcal high-fat diet; WL = Wasabi Leaf/Folium, Wasabia japonica (Miq.) Matsum extracts; IS = Pancreatic islet. All immunostained by <t>avidin-biotin-peroxidase</t> complex. Scale bars = 80 µm.
    Biotinylated Universal Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated universal secondary antibody/product/Vector Laboratories
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated universal secondary antibody - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

    Image Search Results


    OXA- (A and B) and OX1R- (C and D) immunoreactivity in the human normal prostate. (A) Positive cells scattered along the monolayered epithelium of a prostatic follicle. (B) The majority of positive cells visible in this micrograph contain differently stained granular material in the apical portion of their cytoplasm. (C and D) Positive cells lined along the stratified epithelium of three follicles. Some of them are elongated in shape and completely filled with clusters of immunoreactive granules. Avidin–biotin immunohistochemical method. Scale bars: 20 μm.

    Journal: Journal of Anatomy

    Article Title: Expression of orexin A and its receptor 1 in the human prostate

    doi: 10.1111/joa.12030

    Figure Lengend Snippet: OXA- (A and B) and OX1R- (C and D) immunoreactivity in the human normal prostate. (A) Positive cells scattered along the monolayered epithelium of a prostatic follicle. (B) The majority of positive cells visible in this micrograph contain differently stained granular material in the apical portion of their cytoplasm. (C and D) Positive cells lined along the stratified epithelium of three follicles. Some of them are elongated in shape and completely filled with clusters of immunoreactive granules. Avidin–biotin immunohistochemical method. Scale bars: 20 μm.

    Article Snippet: Goat polyclonal anti-OXA (sc-8070) and anti-OX1R antibodies (sc-8073), their respective blocking peptides (sc-8070 P, sc-8073 P), and mouse monoclonal anti-chromogranin A (Chr A) antibody (sc-47714) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); rabbit polyclonal anti-prepro-orexin antibody (AB3096), its blocking peptide (AG774) and monoclonal anti-tubulin antibody (MAB1637) from Chemicon International (Temecula, CA, USA); biotinylated rabbit anti-goat secondary antibodies and avidin–biotin complex (PK-6105) from Vector Laboratories (Burlingame, CA, USA); horseradish peroxidase-conjugated anti-goat IgG (A-5420) and anti-rabbit IgG (A-0545) from Sigma Chemical (St Louis, MO, USA).

    Techniques: Staining, Avidin-Biotin Assay, Immunohistochemistry

    OXA- (A and B) and OX1R- (C and D) immunoreactivity in the human hyperplastic prostate. (A) The basal membrane of an intrafollicular, papillar-like structure is lined by a continuous row of intensely stained cells. (B) A peculiarity often shown by positive basal cells is the presence of a slender cytoplasmic extension (arrows) directed towards the follicular lumen and intermingled between the negative apical cells. (C) Almost all basal cells of the follicular epithelium contain OX1R-immunoreactive material, which completely fills their cytoplasm. (D) Invagination of a prostatic follicle completely lined by positive basal cells. The arrow points to a small cluster of low intensely stained apical cells close in contact with the follicular fluid. Avidin–biotin immunohistochemical method. Scale bars: 20 μm.

    Journal: Journal of Anatomy

    Article Title: Expression of orexin A and its receptor 1 in the human prostate

    doi: 10.1111/joa.12030

    Figure Lengend Snippet: OXA- (A and B) and OX1R- (C and D) immunoreactivity in the human hyperplastic prostate. (A) The basal membrane of an intrafollicular, papillar-like structure is lined by a continuous row of intensely stained cells. (B) A peculiarity often shown by positive basal cells is the presence of a slender cytoplasmic extension (arrows) directed towards the follicular lumen and intermingled between the negative apical cells. (C) Almost all basal cells of the follicular epithelium contain OX1R-immunoreactive material, which completely fills their cytoplasm. (D) Invagination of a prostatic follicle completely lined by positive basal cells. The arrow points to a small cluster of low intensely stained apical cells close in contact with the follicular fluid. Avidin–biotin immunohistochemical method. Scale bars: 20 μm.

    Article Snippet: Goat polyclonal anti-OXA (sc-8070) and anti-OX1R antibodies (sc-8073), their respective blocking peptides (sc-8070 P, sc-8073 P), and mouse monoclonal anti-chromogranin A (Chr A) antibody (sc-47714) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); rabbit polyclonal anti-prepro-orexin antibody (AB3096), its blocking peptide (AG774) and monoclonal anti-tubulin antibody (MAB1637) from Chemicon International (Temecula, CA, USA); biotinylated rabbit anti-goat secondary antibodies and avidin–biotin complex (PK-6105) from Vector Laboratories (Burlingame, CA, USA); horseradish peroxidase-conjugated anti-goat IgG (A-5420) and anti-rabbit IgG (A-0545) from Sigma Chemical (St Louis, MO, USA).

    Techniques: Staining, Avidin-Biotin Assay, Immunohistochemistry

    Distribution of avian influenza virus NP in selected organs of inoculated birds. Distribution of influenza NP in brain ( A , E , I , M ), lung ( B , F , J , N ), spleen ( C , G , K , O ) and heart ( D , H , L , P ) of inoculated chickens of selected viruses at 4 dpi (except for H5N1_H4_T 327 K) as detected by immunohistochemistry using primary polyclonal rabbit anti-NP A/FPV/Rostock/34 antibody (1:750) and a secondary biotinylated goat anti-rabbit IgG (Vector Laboratories, Burlingame, CA, USA) antibody (1:200). 3-amino-9-ethyl-carbazol (red-brown); hematoxylin counterstain (blue); Nomarski contrast; bars A,B,D,E,F,H,I,J,L,M,N,P = 20 µm. Bars C,G,K,O = 50 µm.

    Journal: International Journal of Molecular Sciences

    Article Title: Insertion of Basic Amino Acids in the Hemagglutinin Cleavage Site of H4N2 Avian Influenza Virus (AIV)—Reduced Virus Fitness in Chickens is Restored by Reassortment with Highly Pathogenic H5N1 AIV

    doi: 10.3390/ijms21072353

    Figure Lengend Snippet: Distribution of avian influenza virus NP in selected organs of inoculated birds. Distribution of influenza NP in brain ( A , E , I , M ), lung ( B , F , J , N ), spleen ( C , G , K , O ) and heart ( D , H , L , P ) of inoculated chickens of selected viruses at 4 dpi (except for H5N1_H4_T 327 K) as detected by immunohistochemistry using primary polyclonal rabbit anti-NP A/FPV/Rostock/34 antibody (1:750) and a secondary biotinylated goat anti-rabbit IgG (Vector Laboratories, Burlingame, CA, USA) antibody (1:200). 3-amino-9-ethyl-carbazol (red-brown); hematoxylin counterstain (blue); Nomarski contrast; bars A,B,D,E,F,H,I,J,L,M,N,P = 20 µm. Bars C,G,K,O = 50 µm.

    Article Snippet: Following sections were used for immunohistochemistry using the avidin–biotin–peroxidase complex method (Vector Laboratories Burlingame, CA, USA) with a primary polyclonal rabbit anti-NP antibody (1:750), and a secondary biotinylated goat anti-rabbit IgG (Vector Laboratories, Burlingame, CA, USA) antibody (1:200) as described [ , ].

    Techniques: Immunohistochemistry, Plasmid Preparation

    Sodium/iodide symporter (NIS) expression is primarily restricted to striated ducts in human salivary glands. (A) NIS is expressed at a high level along basolateral membranes of striated duct cells (S), with fewer intercalated (rectangle) and excretory (E) duct cells demonstrating lower NIS levels. Acinar (A) cells do not express NIS. Anti-human NIS antibody, avidin-biotin complex, DAB chromogen, hematoxylin counterstain, bar=25 μm. (B) Distribution and intensity of NIS immunoreactivity among intercalated (ID), striated (SD), and excretory (ED) ductal cells in normal human submandibular, parotid, and minor salivary glands. NIS expression is greater and more frequent in striated ducts, regardless of gland type.

    Journal: Thyroid

    Article Title: Modulation of Sodium/Iodide Symporter Expression in the Salivary Gland

    doi: 10.1089/thy.2012.0571

    Figure Lengend Snippet: Sodium/iodide symporter (NIS) expression is primarily restricted to striated ducts in human salivary glands. (A) NIS is expressed at a high level along basolateral membranes of striated duct cells (S), with fewer intercalated (rectangle) and excretory (E) duct cells demonstrating lower NIS levels. Acinar (A) cells do not express NIS. Anti-human NIS antibody, avidin-biotin complex, DAB chromogen, hematoxylin counterstain, bar=25 μm. (B) Distribution and intensity of NIS immunoreactivity among intercalated (ID), striated (SD), and excretory (ED) ductal cells in normal human submandibular, parotid, and minor salivary glands. NIS expression is greater and more frequent in striated ducts, regardless of gland type.

    Article Snippet: The secondary antibody used was a biotinylated goat anti-rabbit antibody (1:200, BA-1000, Vector Laboratories, Inc., Burlingame, CA) that was incubated for 20 minutes at room temperature.

    Techniques: Expressing, Avidin-Biotin Assay

    NIS expression is reduced in inflamed and neoplastic human salivary glands. (A) NIS expression in striated ducts is decreased in inflammatory salivary glands, as well as in benign and malignant neoplasms of ductal origin. (B) NIS immunoreactivity in inflamed (i, ii) and neoplastic (iii, iv) salivary tissue. NIS expression is variably decreased in striated ducts (S) of chronically inflamed salivary glands (i, ii) , particularly in ducts demonstrating goblet metaplasia (arrows). Neoplastic cells of Warthin's tumor (iii) and mucoepidermoid carcinoma (iv) demonstrate lower NIS level, with immunostaining intensity of 2+ and 1+, respectively. Anti-human NIS antibody, avidin-biotin complex, DAB chromogen, hematoxylin counterstain, bars=25 μm. F, periductal fibrosis; L, lymphocytic inflammation.

    Journal: Thyroid

    Article Title: Modulation of Sodium/Iodide Symporter Expression in the Salivary Gland

    doi: 10.1089/thy.2012.0571

    Figure Lengend Snippet: NIS expression is reduced in inflamed and neoplastic human salivary glands. (A) NIS expression in striated ducts is decreased in inflammatory salivary glands, as well as in benign and malignant neoplasms of ductal origin. (B) NIS immunoreactivity in inflamed (i, ii) and neoplastic (iii, iv) salivary tissue. NIS expression is variably decreased in striated ducts (S) of chronically inflamed salivary glands (i, ii) , particularly in ducts demonstrating goblet metaplasia (arrows). Neoplastic cells of Warthin's tumor (iii) and mucoepidermoid carcinoma (iv) demonstrate lower NIS level, with immunostaining intensity of 2+ and 1+, respectively. Anti-human NIS antibody, avidin-biotin complex, DAB chromogen, hematoxylin counterstain, bars=25 μm. F, periductal fibrosis; L, lymphocytic inflammation.

    Article Snippet: The secondary antibody used was a biotinylated goat anti-rabbit antibody (1:200, BA-1000, Vector Laboratories, Inc., Burlingame, CA) that was incubated for 20 minutes at room temperature.

    Techniques: Expressing, Immunostaining, Avidin-Biotin Assay

    Representative histological images of the insulin- and glucagon-immunoreactive cells in the pancreas, taken from NFD or HFD supplied mice. ( a ) Vehicle (distilled water) 10 mL/kg orally administered mice with NFD supply (Intact control); ( b ) Vehicle 10 mL/kg orally administered mice with HFD supply (HFD control); ( c ) 250 mg/kg of metformin oral administered mice with HFD supply (Metformin); ( d ) WL 200 mg/kg orally administered mice with HFD supply (WL200); ( e ) WL 100 mg/kg orally administered mice with HFD supply (WL100); ( f ) WL 50 mg/kg orally administered mice with HFD supply (WL50). NFD = Normal pellet diet; HFD = 45% Kcal high-fat diet; WL = Wasabi Leaf/Folium, Wasabia japonica (Miq.) Matsum extracts; IS = Pancreatic islet. All immunostained by avidin-biotin-peroxidase complex. Scale bars = 80 µm.

    Journal: Nutrients

    Article Title: Anti-Diabetic Obesity Effects of Wasabia Japonica Matsum Leaf Extract on 45% Kcal High-Fat Diet-Fed Mice

    doi: 10.3390/nu12092837

    Figure Lengend Snippet: Representative histological images of the insulin- and glucagon-immunoreactive cells in the pancreas, taken from NFD or HFD supplied mice. ( a ) Vehicle (distilled water) 10 mL/kg orally administered mice with NFD supply (Intact control); ( b ) Vehicle 10 mL/kg orally administered mice with HFD supply (HFD control); ( c ) 250 mg/kg of metformin oral administered mice with HFD supply (Metformin); ( d ) WL 200 mg/kg orally administered mice with HFD supply (WL200); ( e ) WL 100 mg/kg orally administered mice with HFD supply (WL100); ( f ) WL 50 mg/kg orally administered mice with HFD supply (WL50). NFD = Normal pellet diet; HFD = 45% Kcal high-fat diet; WL = Wasabi Leaf/Folium, Wasabia japonica (Miq.) Matsum extracts; IS = Pancreatic islet. All immunostained by avidin-biotin-peroxidase complex. Scale bars = 80 µm.

    Article Snippet: This was followed by incubation with ABC reagents (Vectastain Elite ABC Kit, Vector Lab., Burlingame, CA, USA) and biotinylated universal secondary antibody (Vector Lab., Burlingame, CA, USA) in a humidity chamber for 1 h at room temperature.

    Techniques: Mouse Assay, Avidin-Biotin Assay