biotinylated anti rabbit secondary antibody (Vector Laboratories)
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Vector Laboratories manufactures this product 
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Name:
Biotinylated Rabbit Anti Rat IgG Antibody
Description:
Biotinylated Rabbit Anti Rat IgG Antibody is prepared using proprietary immunization schedules that produce high affinity antibodies The antibodies are then purified by affinity chromatography and cross reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining ELISAs and blots Biotinylated Rabbit Anti Rat IgG H L is supplied in lyophilized form and can be reconstituted with 1 ml water With some exceptions the recommended dilution for most applications is 1 200 H L indicates the antibody recognizes both heavy and light chains This antibody is included in the VECTASTAIN ABC kits
Catalog Number:
ba-4000
Price:
None
Category:
Antibodies
Reactivity:
Rat
Size:
1 5 mg
Host:
Rabbit
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Structured Review
Vector Laboratories
biotinylated anti rabbit secondary antibody
Biotinylated Rabbit Anti Rat IgG Antibody is prepared using proprietary immunization schedules that produce high affinity antibodies The antibodies are then purified by affinity chromatography and cross reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining ELISAs and blots Biotinylated Rabbit Anti Rat IgG H L is supplied in lyophilized form and can be reconstituted with 1 ml water With some exceptions the recommended dilution for most applications is 1 200 H L indicates the antibody recognizes both heavy and light chains This antibody is included in the VECTASTAIN ABC kits
https://www.bioz.com/result/biotinylated anti rabbit secondary antibody/product/Vector Laboratories
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Biotinylated Rabbit Anti Rat IgG Antibody is prepared using proprietary immunization schedules that produce high affinity antibodies The antibodies are then purified by affinity chromatography and cross reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining ELISAs and blots Biotinylated Rabbit Anti Rat IgG H L is supplied in lyophilized form and can be reconstituted with 1 ml water With some exceptions the recommended dilution for most applications is 1 200 H L indicates the antibody recognizes both heavy and light chains This antibody is included in the VECTASTAIN ABC kits
https://www.bioz.com/result/biotinylated anti rabbit secondary antibody/product/Vector Laboratories
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
biotinylated anti rabbit secondary antibody - by Bioz Stars,
2021-03
86/100 stars
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Incubation:Article Title: Immunohistochemical analysis of macroautophagy Article Snippet: Subsequently, the primary antibody was applied overnight in a damp box, followed by 2 × 5 min immersion in staining buffer. .. Next, the slides were incubated for 30 min with Article Title: Human epidermal growth factor receptor 2 overexpression in breast cancer of patients with anti-Yo-associated paraneoplastic cerebellar degeneration Article Snippet: To determine the frequency of cdr2 expression in HER2-positive breast tumors, paraffin sections of 8 HER2-positive breast tumors from patients without PCD were deparaffinized in xylene, rehydrated in alcohol, washed in tap water, and heated for 10 min in a pressure-cooking oven in 0.1 M sodium citrate buffer (pH, 6.0). .. After inhibition of endogenous peroxidase with 0.3% hydrogen peroxide in phosphate-buffered saline (PBS) for 15 min, sections were sequentially incubated with undiluted normal human serum or a human serum with high titers of Yo antibodies for 2 hours at room temperature, followed by Article Title: Abnormalities of Thymic Stroma may Contribute to Immune Dysregulation in Murine Models of Leaky Severe Combined Immunodeficiency Article Snippet: After washes, single immunostains were revealed using the ChemMATE Envision Rabbit/Mouse (DAKO Cytomation, Glostrup, Denmark) or NovoLinkTM Polymer Detection System (NovocastraTM Laboratories Ltd, Newcastle Upon Tyne, United Kingdom) followed by diaminobenzidine (DAB) as chromogen and Hematoxylin as counterstain. .. The same procedure was applied to double immunofluorescence stainings prior to the incubation with a secondary swine anti-rabbit FITC-conjugated antibody (1:30; DAKO Cytomation, Glostrup, Denmark) for CK5 and a Staining:Article Title: Immunohistochemical analysis of macroautophagy Article Snippet: Subsequently, the primary antibody was applied overnight in a damp box, followed by 2 × 5 min immersion in staining buffer. .. Next, the slides were incubated for 30 min with Article Title: The specific JNK inhibitor SP600125 targets tumour necrosis factor-? production and epithelial cell apoptosis in acute murine colitis Article Snippet: The following antibodies were used at the indicated dilutions: PpJNK (1 : 100, NEB, Pickering, CAN), anti-rat F4/80 (1 : 100). .. Sections were stained using a Vectastain ABC elite kit and Avidin-Biotin Assay:Article Title: Assembly with the NR1 Subunit Is Required for Surface Expression of NR3A-Containing NMDA Receptors Article Snippet: For immunostaining with rabbit anti-calreticulin (1:200) and mouse anti-Grp78 (1:200; Stressgen Biotechnologies, Victoria, British Columbia, Canada) antibodies, cells were permeabilized with 0.1% Triton X-100 in PBS for 10 min before addition of the primary antibody. .. Article Title: Anti-α4β1 Integrin Antibodies Attenuated Brain Inflammatory Changes in a Mouse Model of Alzheimer’s Disease Article Snippet: All mouse cytokine ELISA kits were obtained from R & D Systems (Minneapolis, MN). .. Elite Vectastain ABC avidin and biotin kits, Article Title: Trafficking to the Plasma Membrane of the Seven-Transmembrane Protein Encoded by Human Herpesvirus 6 U51 Gene Involves a Cell-Specific Function Present in T Lymphocytes Article Snippet: Infection of CBMCs with HHV-6 was monitored routinely by immunofluorescence with monoclonal antibody (MAb) 2D10 to glycoprotein B (gB), as described elsewhere ( ). .. Commercially available antibodies were antihemagglutinin (anti-HA) antibody (AntiXpress) (Invitrogen), anti-CC-chemokine receptor 5 (anti-CCR5) MAb LS100/2D7 (R & D Systems, Abingdon, United Kingdom), fluorescein isothiocyanate (FITC)-conjugated anti-mouse antibody (Jackson), FITC-conjugated anti-rabbit antibody (Dako), Article Title: The specific JNK inhibitor SP600125 targets tumour necrosis factor-? production and epithelial cell apoptosis in acute murine colitis Article Snippet: The following antibodies were used at the indicated dilutions: PpJNK (1 : 100, NEB, Pickering, CAN), anti-rat F4/80 (1 : 100). .. Sections were stained using a Vectastain ABC elite kit and Inhibition:Article Title: Human epidermal growth factor receptor 2 overexpression in breast cancer of patients with anti-Yo-associated paraneoplastic cerebellar degeneration Article Snippet: To determine the frequency of cdr2 expression in HER2-positive breast tumors, paraffin sections of 8 HER2-positive breast tumors from patients without PCD were deparaffinized in xylene, rehydrated in alcohol, washed in tap water, and heated for 10 min in a pressure-cooking oven in 0.1 M sodium citrate buffer (pH, 6.0). .. After inhibition of endogenous peroxidase with 0.3% hydrogen peroxide in phosphate-buffered saline (PBS) for 15 min, sections were sequentially incubated with undiluted normal human serum or a human serum with high titers of Yo antibodies for 2 hours at room temperature, followed by Plasmid Preparation:Article Title: Anti-α4β1 Integrin Antibodies Attenuated Brain Inflammatory Changes in a Mouse Model of Alzheimer’s Disease Article Snippet: All mouse cytokine ELISA kits were obtained from R & D Systems (Minneapolis, MN). .. Elite Vectastain ABC avidin and biotin kits, Immunofluorescence:Article Title: Abnormalities of Thymic Stroma may Contribute to Immune Dysregulation in Murine Models of Leaky Severe Combined Immunodeficiency Article Snippet: After washes, single immunostains were revealed using the ChemMATE Envision Rabbit/Mouse (DAKO Cytomation, Glostrup, Denmark) or NovoLinkTM Polymer Detection System (NovocastraTM Laboratories Ltd, Newcastle Upon Tyne, United Kingdom) followed by diaminobenzidine (DAB) as chromogen and Hematoxylin as counterstain. .. The same procedure was applied to double immunofluorescence stainings prior to the incubation with a secondary swine anti-rabbit FITC-conjugated antibody (1:30; DAKO Cytomation, Glostrup, Denmark) for CK5 and a Article Title: The specific JNK inhibitor SP600125 targets tumour necrosis factor-? production and epithelial cell apoptosis in acute murine colitis Article Snippet: The following antibodies were used at the indicated dilutions: PpJNK (1 : 100, NEB, Pickering, CAN), anti-rat F4/80 (1 : 100). .. Sections were stained using a Vectastain ABC elite kit and Labeling:Article Title: Endocytosis of a Glycosylphosphatidylinositol-anchored Protein via Clathrin-coated Vesicles, Sorting by Default in Endosomes, and Exocytosis via RAB11-positive Carriers V⃞ Article Snippet: For double labeling (Figures , inset; ), the first antibody was generally detected by PAG-10 or PAG-6; after a block with a rabbit normal IgG (1:400), the second antibody was detected by PAG-10 or PAG-15. .. In one case ( , inset), clathrin was first labeled with rabbit anti-TbCLH antibodies and PAG-6, whereas VSG was subsequently detected using |
