Journal: Malaria Journal

Article Title: Pre-existing Schistosoma japonicum infection alters the immune response to Plasmodium berghei infection in C57BL/6 mice

doi: 10.1186/1475-2875-12-322

Figure Lengend Snippet: Mice in Plasmodium berghei ANKA-mono-infection group and Schistosoma japonicum-P. berghei ANKA-co-infection group were compared at different time points according to data obtained by flow cytometry. Proportion of splenic DCs subpopulation, CD11c + CD11b + and CD11c + CD45R⁄ B220 + , were analysed in A and B , with absolute number of these DCs present in C and D . Proportion of splenic CD11c + DCs expressing TLR4 , TLR9, MHC class II, and CD86 of the two groups were compared in E , F , G , H , respectively. MFI of TLR4, TLR9, MHC class II, and CD86, which were expressed on CD11c + CD11b + DCs and CD11c + CD45R⁄ B220 + DCs were present in I and J . At each time point, at least three mice per group were sacrificed. Bars represent the mean values ± SD. Student t test by comparisons between the two groups was performed with * indicating P <0.05 and # indicating P <0.01.

Article Snippet: After fixation and permeabilization using staining buffer reagents as instructed by the manufacturer, cells were incubated with biotinylated anti-mouse TLR9 (clone 5G5, Hycult Biotechnology (HBT), Uden, The Netherlands) followed by PE-conjugated streptavidin (Biolegend, San Diego, CA, USA).

Techniques: Infection, Flow Cytometry, Expressing