biotinylated anti mouse igg  (Vector Laboratories)


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    Name:
    Mouse on Mouse M O M Biotinylated Anti Mouse Ig Reagent
    Description:
    M O M Mouse on Mouse Anti Mouse Reagent is a specially modified secondary antibody that has been optimized specifically for use with the Vector M O M Immunodetection Kit components This is the same reagent contained in the Vector M O M Kits Cat Nos PK 2200 FMK 2201 and BMK 2202 The Vector M O M Immunodetection Kits are specifically designed to reduce endogenous mouse IgG staining when using mouse primary antibodies on mouse tissue The inability of an anti mouse secondary antibody to distinguish between a primary antibody produced in mice and the endogenous mouse immunoglobulins present in mouse tissue results in high background staining which obscures specific staining This problem can be essentially eliminated by using a Vector M O M Kit The result is clear crisp specific staining of the antigens of interest Excellent staining results for a once difficult application have now become routine with the Vector M O M System
    Catalog Number:
    mkb-2225
    Price:
    None
    Category:
    Antibodies
    Reactivity:
    Mouse
    Size:
    0 1 ml
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    Structured Review

    Vector Laboratories biotinylated anti mouse igg
    Mouse on Mouse M O M Biotinylated Anti Mouse Ig Reagent
    M O M Mouse on Mouse Anti Mouse Reagent is a specially modified secondary antibody that has been optimized specifically for use with the Vector M O M Immunodetection Kit components This is the same reagent contained in the Vector M O M Kits Cat Nos PK 2200 FMK 2201 and BMK 2202 The Vector M O M Immunodetection Kits are specifically designed to reduce endogenous mouse IgG staining when using mouse primary antibodies on mouse tissue The inability of an anti mouse secondary antibody to distinguish between a primary antibody produced in mice and the endogenous mouse immunoglobulins present in mouse tissue results in high background staining which obscures specific staining This problem can be essentially eliminated by using a Vector M O M Kit The result is clear crisp specific staining of the antigens of interest Excellent staining results for a once difficult application have now become routine with the Vector M O M System
    https://www.bioz.com/result/biotinylated anti mouse igg/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated anti mouse igg - by Bioz Stars, 2021-03
    99/100 stars

    Images

    1) Product Images from "Antibodies to several conformation-dependent epitopes of gp120/gp41 inhibit CCR-5-dependent cell-to-cell fusion mediated by the native envelope glycoprotein of a primary macrophage-tropic HIV-1 isolate"

    Article Title: Antibodies to several conformation-dependent epitopes of gp120/gp41 inhibit CCR-5-dependent cell-to-cell fusion mediated by the native envelope glycoprotein of a primary macrophage-tropic HIV-1 isolate

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi:

    Formation of syncytia in CD4 + HeLa cells expressing CCR-5 and gp120/gp41 from a macrophage-tropic primary HIV-1 isolate. P4 (CCR-5 − ) ( 1 , 4 , 7 , and 8 ) or P4P (CCR-5 + ) ( 2 , 3 , 5 , and 6 ) HeLa cells (as indicated) were infected with defective recombinant SFV-LAI ( 1 , 2 , and 7 ), SFV-BX08 ( 4 , 5 , and 8 ) or SFV-LacZ ( 3 ). Control cells were left uninfected ( 6 ). After 12 h incubation at 37°C, cells were fixed with 0.5% (vol/vol) glutaraldehyde and stained with Giemsa ( 1 - 6 ) or reacted with mAb 2G12 ( 7 and 8 ) as primary antibody, followed by biotinylated anti-mouse IgG then avidin and biotinylated horseradish peroxidase H (Vectastain ABC kit). The cells were examined by microscopy. (×125.)
    Figure Legend Snippet: Formation of syncytia in CD4 + HeLa cells expressing CCR-5 and gp120/gp41 from a macrophage-tropic primary HIV-1 isolate. P4 (CCR-5 − ) ( 1 , 4 , 7 , and 8 ) or P4P (CCR-5 + ) ( 2 , 3 , 5 , and 6 ) HeLa cells (as indicated) were infected with defective recombinant SFV-LAI ( 1 , 2 , and 7 ), SFV-BX08 ( 4 , 5 , and 8 ) or SFV-LacZ ( 3 ). Control cells were left uninfected ( 6 ). After 12 h incubation at 37°C, cells were fixed with 0.5% (vol/vol) glutaraldehyde and stained with Giemsa ( 1 - 6 ) or reacted with mAb 2G12 ( 7 and 8 ) as primary antibody, followed by biotinylated anti-mouse IgG then avidin and biotinylated horseradish peroxidase H (Vectastain ABC kit). The cells were examined by microscopy. (×125.)

    Techniques Used: Expressing, Infection, Recombinant, Incubation, Staining, Avidin-Biotin Assay, Microscopy

    Related Articles

    Incubation:

    Article Title: Co-treatment with therapeutic neural stem cells expressing carboxyl esterase and CPT-11 inhibit growth of primary and metastatic lung cancers in mice
    Article Snippet: Samples were subsequently incubated with a primary mouse monoclonal antibody against proliferating cell nuclear antigen (PCNA, 1:100, Abcam, plc., Cambridge, UK) for overnight at 4°C. .. After four washes for 10 min in PBS-Tween, the slides were incubated in the biotinylated anti-mouse secondary antibody (1:500 dilution, Vector Laboratories) solution for 30 min at 37°C. .. To form the immunoreactive complex, ABC kit reagent (Vectastain Universal Elite ABC kit, Vector Laboratories) was treated at each slide for 30 min. After DAB substrate (Sigma-Aldrich Co.) and hematoxylin staining solution treatment, all slides were detected under a BX51 light microscope for digital photography.

    Article Title: Inflammatory properties of inhibitor of DNA binding 1 secreted by synovial fibroblasts in rheumatoid arthritis
    Article Snippet: The tissue sections were blocked with 5 % donkey serum and 20 % fetal bovine serum (FBS) in phosphate-buffered saline (PBS) at 37 °C for 1 h. The sections were then incubated with either mouse anti-human Id1 antibody (Abcam, Cambridge, MA, USA, 10 μg/mL), rabbit anti-mouse Id1 antibody (CalBioreagents, San Mateo, CA, USA, 10 μg/mL), or purified nonspecific mouse and rabbit immunoglobulin G (IgG) (Thermo Fisher Scientific, Waltham, MA, USA) for 1 h at 37 °C in blocking buffer. .. After washing, tissues were incubated with a biotinylated anti-mouse or anti-rabbit secondary antibody (Vector Laboratories, Burlingame, CA, USA, 10 μg/mL) for 1 h at 37 °C in blocking buffer. .. Vectastain ABC kit (Vector Laboratories) was used to detect the antibodies on the tissues, following manufacturer’s protocols.

    Article Title: Human Osteogenesis Involves Differentiation-Dependent Increases in the Morphogenically Active 3? Alternative Splicing Variant of Acetylcholinesterase
    Article Snippet: Findings were expressed as mean ± standard error, and analysis of variance (ANOVA) was performed with the superANOVA statistical package (Abacus Concepts, Inc., Berkeley, Calif.). .. For immunohistochemistry, cryocut sections were incubated with human-specific anti-AChE monoclonal antibody 101-1 (primary antibody) ( ) and biotinylated anti-mouse antibody (secondary antibody) (Vectastain; Vector Laboratories, Burlingame, Calif.); detection was carried out with 3,3′-diaminobenzidine and urea-H2 O2 as instructed by the manufacturer (Sigma). .. Cytochemical staining for AChE activity on nonfixed cells grown on glass slides and on cryocut sections, as well as determination of hydrolysis rates of acetylthiocholine iodide in homogenates, was performed essentially as detailed elsewhere ( ) for the noted time periods.

    Article Title: Host-Specific Glycans Are Correlated with Susceptibility to Infection by Lagoviruses, but Not with Their Virulence
    Article Snippet: B/EBHS/6-infected liver homogenate diluted 1/5 in PBS-1% BSA was then added and incubated at 4°C overnight. .. After 3 washes with PBS, monoclonal anti-EBHSV antibody (5F5; a kind gift from L. Capucci, IZSLER, Brescia, Italy) was added at 1/100 dilution for 2 h at 37°C, followed by 3 washes with PBS and incubation with biotinylated anti-mouse antibody (Vector Laboratories) at 1/1,000 dilution for 2 h at 37°C. .. The sequence for the capsid protein gene of isolate B-EBHS-6 is available in GenBank under accession number .

    Article Title: Anatomical Plasticity of the Distal Forelimb Projection of the Ventral Premotor Cortex Four weeks After Primary Motor Cortex Injury
    Article Snippet: The first series was incubated in 5% normal goat serum rinse buffer overnight then incubated in ABC solution for 4 hours then reacted with 3-3” diaminobenzidine (DAB) to visualize BDA ( ; ) ( , ), the second series was incubated with a monoclonal primary antibody raised in mouse directed against the neuronal marker NeuN (Chemicon, Temecula, CA) for 48 hours at a 1:1000 ratio. .. Following the 48 hour primary antibody incubation, tissue sections were rinsed and incubated at room temperature in a secondary biotinylated anti-mouse antibody for 4 hours (Vector Laboratories, Burlingame, CA). .. This series was then incubated with ABC solution and reacted with Vector SG (Vector Laboratories, Burlingame,CA) ( ).

    Infection:

    Article Title: Antibodies to several conformation-dependent epitopes of gp120/gp41 inhibit CCR-5-dependent cell-to-cell fusion mediated by the native envelope glycoprotein of a primary macrophage-tropic HIV-1 isolate
    Article Snippet: .. Virus stocks were titered on BHK cells, infection was detected by immunohistostaining with mAbs 41A and F5.5 (1 μg/ml) as primary antibodies, followed by biotinylated anti-mouse IgG then avidin and biotinylated horseradish peroxidase H (Vectastain ABC kit; Vector Laboratories). .. P4 (CCR-5− ) or P4P (CCR-5+ ) HeLa cells, at a density of 2 × 105 cells per well in a 24-multiwell plate, were washed with DMEM without serum and infected with defective recombinant SFV–LAI, SFV–BX08, or SFV–LacZ at a multiplicity of infection of 3.6 infectious particles per cell (as measured on BHK cells).

    Avidin-Biotin Assay:

    Article Title: Antibodies to several conformation-dependent epitopes of gp120/gp41 inhibit CCR-5-dependent cell-to-cell fusion mediated by the native envelope glycoprotein of a primary macrophage-tropic HIV-1 isolate
    Article Snippet: .. Virus stocks were titered on BHK cells, infection was detected by immunohistostaining with mAbs 41A and F5.5 (1 μg/ml) as primary antibodies, followed by biotinylated anti-mouse IgG then avidin and biotinylated horseradish peroxidase H (Vectastain ABC kit; Vector Laboratories). .. P4 (CCR-5− ) or P4P (CCR-5+ ) HeLa cells, at a density of 2 × 105 cells per well in a 24-multiwell plate, were washed with DMEM without serum and infected with defective recombinant SFV–LAI, SFV–BX08, or SFV–LacZ at a multiplicity of infection of 3.6 infectious particles per cell (as measured on BHK cells).

    Blocking Assay:

    Article Title: Inflammatory properties of inhibitor of DNA binding 1 secreted by synovial fibroblasts in rheumatoid arthritis
    Article Snippet: The tissue sections were blocked with 5 % donkey serum and 20 % fetal bovine serum (FBS) in phosphate-buffered saline (PBS) at 37 °C for 1 h. The sections were then incubated with either mouse anti-human Id1 antibody (Abcam, Cambridge, MA, USA, 10 μg/mL), rabbit anti-mouse Id1 antibody (CalBioreagents, San Mateo, CA, USA, 10 μg/mL), or purified nonspecific mouse and rabbit immunoglobulin G (IgG) (Thermo Fisher Scientific, Waltham, MA, USA) for 1 h at 37 °C in blocking buffer. .. After washing, tissues were incubated with a biotinylated anti-mouse or anti-rabbit secondary antibody (Vector Laboratories, Burlingame, CA, USA, 10 μg/mL) for 1 h at 37 °C in blocking buffer. .. Vectastain ABC kit (Vector Laboratories) was used to detect the antibodies on the tissues, following manufacturer’s protocols.

    Immunohistochemistry:

    Article Title: Human Osteogenesis Involves Differentiation-Dependent Increases in the Morphogenically Active 3? Alternative Splicing Variant of Acetylcholinesterase
    Article Snippet: Findings were expressed as mean ± standard error, and analysis of variance (ANOVA) was performed with the superANOVA statistical package (Abacus Concepts, Inc., Berkeley, Calif.). .. For immunohistochemistry, cryocut sections were incubated with human-specific anti-AChE monoclonal antibody 101-1 (primary antibody) ( ) and biotinylated anti-mouse antibody (secondary antibody) (Vectastain; Vector Laboratories, Burlingame, Calif.); detection was carried out with 3,3′-diaminobenzidine and urea-H2 O2 as instructed by the manufacturer (Sigma). .. Cytochemical staining for AChE activity on nonfixed cells grown on glass slides and on cryocut sections, as well as determination of hydrolysis rates of acetylthiocholine iodide in homogenates, was performed essentially as detailed elsewhere ( ) for the noted time periods.

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    Vector Laboratories goat anti mouse α tran
    Western blot findings of <t>α-tran</t> and α-gust in the small intestine of CTR, T1 and T2 animals. The values are shown with different superscripts (A, B) for p
    Goat Anti Mouse α Tran, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse α tran/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    99
    Vector Laboratories biotinylated antirat igg
    TECs exhibit different cellular distributions of endothelial molecules than normal MHECs. TECs or MHECs were plated at subculture 2 onto glass coverslips precoated with either OnFN or hFN, respectively. At 2 days postconfluence, the cells were washed with DPBS + and fixed in ice-cold methanol for 5 min at -20°C. Cell surface adhesion molecules (CD31, β-catenin, ICAM-2, and VCAM-1) were stained with specific antibodies as described in Materials and Methods section and detected with <t>antirat</t> or antirabbit <t>IgG</t> conjugated to Texas Red or FITC. Fluorescence images were captured on an Axiovert inverted microscope equipped for fluorescence using a cooled CCD camera and IP Laboratories software. All images displayed were captured with a x 40 objective. These images are representative of three separate cultures of both MHECs and TECs generated from different source tissues at different times. Images of MHECs are shown in the left hand panels (a,c,e,g) and of TECs in the right hand panels (b,d,f,g) with staining for CD31 (a,b), β-catenin (c,d), ICAM-2 (e,f), and VCAM-1 (g,h). Junctional staining of CD31 and β-catenin was discontinuous in TECs, indicating the presence of fewer cell-cell junctions. In addition, VCAM-1 did not localize to cell-cell junctions in TECs. In contrast, normal MHECs showed continuous junctional staining for CD31, β-catenin, and VCAM-1. There was little difference in distribution of ICAM-2 between MHECs and TECs.
    Biotinylated Antirat Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated antirat igg/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    94
    Vector Laboratories ntsr1
    <t>NTS/NTSR1</t> complex enhanced experimental tumor growth (A) Tumor growth generated by LNM35, LNM-R and LMN-F cells xenografted into nude mice. One million cells from LNM35, LNM-R, LNM-F, or a mixture of LNM-R and LNM-F (50/50) were subcutaneously injected in 24, 36, 34, or 12 nude mice, respectively. (B) Typical immunohistochemistry for NTSR1 (left) or NTS (right) for tumors generated from LNM-R (top) or LNM-F (bottom) cells. Significant differences at *** P
    Ntsr1, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ntsr1/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ntsr1 - by Bioz Stars, 2021-03
    94/100 stars
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    Image Search Results


    Western blot findings of α-tran and α-gust in the small intestine of CTR, T1 and T2 animals. The values are shown with different superscripts (A, B) for p

    Journal: Animals : an Open Access Journal from MDPI

    Article Title: Dietary Verbascoside Influences Gut Morphology and the Expression of α-Transducin and α-Gustducin in the Small Intestine of Weaned Piglets Exposed to n-6 Polyunsaturated Fatty Acids-Induced Oxidative Stress

    doi: 10.3390/ani9010020

    Figure Lengend Snippet: Western blot findings of α-tran and α-gust in the small intestine of CTR, T1 and T2 animals. The values are shown with different superscripts (A, B) for p

    Article Snippet: De-waxed and rehydrated paraffin sections were incubated with the first-step primary antibody, 1:10 goat anti-mouse α-tran, for 24 h at 18–20 °C, then washed in Tris-buffered saline solution (TBS 0.05 M Tris/HCl, 0.15 M NaCl pH = 7.6), and subsequently treated with the avidin–biotin blocking kit solution (Vector Laboratories Inc., Burlingame, CA, USA).

    Techniques: Western Blot

    Percentage of α-tran/chromoA or ghre-IR cells. The values are shown with different superscripts (a, b) for p

    Journal: Animals : an Open Access Journal from MDPI

    Article Title: Dietary Verbascoside Influences Gut Morphology and the Expression of α-Transducin and α-Gustducin in the Small Intestine of Weaned Piglets Exposed to n-6 Polyunsaturated Fatty Acids-Induced Oxidative Stress

    doi: 10.3390/ani9010020

    Figure Lengend Snippet: Percentage of α-tran/chromoA or ghre-IR cells. The values are shown with different superscripts (a, b) for p

    Article Snippet: De-waxed and rehydrated paraffin sections were incubated with the first-step primary antibody, 1:10 goat anti-mouse α-tran, for 24 h at 18–20 °C, then washed in Tris-buffered saline solution (TBS 0.05 M Tris/HCl, 0.15 M NaCl pH = 7.6), and subsequently treated with the avidin–biotin blocking kit solution (Vector Laboratories Inc., Burlingame, CA, USA).

    Techniques:

    Immunofluorescence findings of the enteroendocrine cells of the proximal duodenum: representative images. Double staining indicates the cytoplasmic localization of chromogranin A, or ghrelin and α-tran. ( a ) CTR, the red color is α-tran; ( b ) CTR, the green color is chromogranin A; ( c ) CTR, the red color is α-tran, the green color is chromogranin A and the yellow color is colocalization. ( d ) T2, the red color is α-tran; ( e ) T2, the green is color ghrelin; ( c ) T2, the red color is α-tran, the green is color ghrelin and yellow color is colocalization.

    Journal: Animals : an Open Access Journal from MDPI

    Article Title: Dietary Verbascoside Influences Gut Morphology and the Expression of α-Transducin and α-Gustducin in the Small Intestine of Weaned Piglets Exposed to n-6 Polyunsaturated Fatty Acids-Induced Oxidative Stress

    doi: 10.3390/ani9010020

    Figure Lengend Snippet: Immunofluorescence findings of the enteroendocrine cells of the proximal duodenum: representative images. Double staining indicates the cytoplasmic localization of chromogranin A, or ghrelin and α-tran. ( a ) CTR, the red color is α-tran; ( b ) CTR, the green color is chromogranin A; ( c ) CTR, the red color is α-tran, the green color is chromogranin A and the yellow color is colocalization. ( d ) T2, the red color is α-tran; ( e ) T2, the green is color ghrelin; ( c ) T2, the red color is α-tran, the green is color ghrelin and yellow color is colocalization.

    Article Snippet: De-waxed and rehydrated paraffin sections were incubated with the first-step primary antibody, 1:10 goat anti-mouse α-tran, for 24 h at 18–20 °C, then washed in Tris-buffered saline solution (TBS 0.05 M Tris/HCl, 0.15 M NaCl pH = 7.6), and subsequently treated with the avidin–biotin blocking kit solution (Vector Laboratories Inc., Burlingame, CA, USA).

    Techniques: Immunofluorescence, Double Staining

    TECs exhibit different cellular distributions of endothelial molecules than normal MHECs. TECs or MHECs were plated at subculture 2 onto glass coverslips precoated with either OnFN or hFN, respectively. At 2 days postconfluence, the cells were washed with DPBS + and fixed in ice-cold methanol for 5 min at -20°C. Cell surface adhesion molecules (CD31, β-catenin, ICAM-2, and VCAM-1) were stained with specific antibodies as described in Materials and Methods section and detected with antirat or antirabbit IgG conjugated to Texas Red or FITC. Fluorescence images were captured on an Axiovert inverted microscope equipped for fluorescence using a cooled CCD camera and IP Laboratories software. All images displayed were captured with a x 40 objective. These images are representative of three separate cultures of both MHECs and TECs generated from different source tissues at different times. Images of MHECs are shown in the left hand panels (a,c,e,g) and of TECs in the right hand panels (b,d,f,g) with staining for CD31 (a,b), β-catenin (c,d), ICAM-2 (e,f), and VCAM-1 (g,h). Junctional staining of CD31 and β-catenin was discontinuous in TECs, indicating the presence of fewer cell-cell junctions. In addition, VCAM-1 did not localize to cell-cell junctions in TECs. In contrast, normal MHECs showed continuous junctional staining for CD31, β-catenin, and VCAM-1. There was little difference in distribution of ICAM-2 between MHECs and TECs.

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Murine Lewis Lung Carcinoma-Derived Endothelium Expresses Markers of Endothelial Activation and Requires Tumor-Specific Extracellular Matrix In Vitro 1

    doi:

    Figure Lengend Snippet: TECs exhibit different cellular distributions of endothelial molecules than normal MHECs. TECs or MHECs were plated at subculture 2 onto glass coverslips precoated with either OnFN or hFN, respectively. At 2 days postconfluence, the cells were washed with DPBS + and fixed in ice-cold methanol for 5 min at -20°C. Cell surface adhesion molecules (CD31, β-catenin, ICAM-2, and VCAM-1) were stained with specific antibodies as described in Materials and Methods section and detected with antirat or antirabbit IgG conjugated to Texas Red or FITC. Fluorescence images were captured on an Axiovert inverted microscope equipped for fluorescence using a cooled CCD camera and IP Laboratories software. All images displayed were captured with a x 40 objective. These images are representative of three separate cultures of both MHECs and TECs generated from different source tissues at different times. Images of MHECs are shown in the left hand panels (a,c,e,g) and of TECs in the right hand panels (b,d,f,g) with staining for CD31 (a,b), β-catenin (c,d), ICAM-2 (e,f), and VCAM-1 (g,h). Junctional staining of CD31 and β-catenin was discontinuous in TECs, indicating the presence of fewer cell-cell junctions. In addition, VCAM-1 did not localize to cell-cell junctions in TECs. In contrast, normal MHECs showed continuous junctional staining for CD31, β-catenin, and VCAM-1. There was little difference in distribution of ICAM-2 between MHECs and TECs.

    Article Snippet: Serial sections were stained with antibodies to CD31, CD102, CD144, CD106, Flk-1, and CD62E at 1 µg/ml, detected with biotinylated antirat IgG, and developed with the NovaRED substrate kit (Vector Laboratories).

    Techniques: Staining, Fluorescence, Inverted Microscopy, Software, Generated

    Comparison of untreated KAT-4 and Capan-2 carcinoma models. a Hematoxylin and Eosin and Sirius red staining in KAT-4 and Capan-2 carcinomas (bars = 100 μm). Trichrome staining (bars = 20 μm) and immunofluorescence staining with biotinylated 3G9 antibody (green) shows that the expression of integrin α V β 6 is located at the cell membrane in both KAT-4 and Capan-2 carcinomas (cell nuclei stained with DAPI, blue; bars = 50 μm). b Collagen content in untreated KAT-4 ( n = 4) and Capan-2 ( n = 5) carcinomas, represented by hydroxyproline mg/g wet weight. c Average growth of untreated KAT-4 ( n = 8) and Capan-2 tumors ( n = 7), represented in mm 3 measured externally (length x width x height)

    Journal: Cell Communication and Signaling : CCS

    Article Title: Inhibition of integrin αVβ6 changes fibril thickness of stromal collagen in experimental carcinomas

    doi: 10.1186/s12964-018-0249-7

    Figure Lengend Snippet: Comparison of untreated KAT-4 and Capan-2 carcinoma models. a Hematoxylin and Eosin and Sirius red staining in KAT-4 and Capan-2 carcinomas (bars = 100 μm). Trichrome staining (bars = 20 μm) and immunofluorescence staining with biotinylated 3G9 antibody (green) shows that the expression of integrin α V β 6 is located at the cell membrane in both KAT-4 and Capan-2 carcinomas (cell nuclei stained with DAPI, blue; bars = 50 μm). b Collagen content in untreated KAT-4 ( n = 4) and Capan-2 ( n = 5) carcinomas, represented by hydroxyproline mg/g wet weight. c Average growth of untreated KAT-4 ( n = 8) and Capan-2 tumors ( n = 7), represented in mm 3 measured externally (length x width x height)

    Article Snippet: Sections were blocked in 5% swine serum (Sigma) and 2% BSA (Sigma), incubated with biotinylated 3G9 antibody and then with Fluorescein Avidin D (Vector Labs).

    Techniques: Staining, Immunofluorescence, Expressing

    NTS/NTSR1 complex enhanced experimental tumor growth (A) Tumor growth generated by LNM35, LNM-R and LMN-F cells xenografted into nude mice. One million cells from LNM35, LNM-R, LNM-F, or a mixture of LNM-R and LNM-F (50/50) were subcutaneously injected in 24, 36, 34, or 12 nude mice, respectively. (B) Typical immunohistochemistry for NTSR1 (left) or NTS (right) for tumors generated from LNM-R (top) or LNM-F (bottom) cells. Significant differences at *** P

    Journal: Oncotarget

    Article Title: Neurotensin (NTS) and its receptor (NTSR1) causes EGFR, HER2 and HER3 over-expression and their autocrine/paracrine activation in lung tumors, confirming responsiveness to erlotinib

    doi:

    Figure Lengend Snippet: NTS/NTSR1 complex enhanced experimental tumor growth (A) Tumor growth generated by LNM35, LNM-R and LMN-F cells xenografted into nude mice. One million cells from LNM35, LNM-R, LNM-F, or a mixture of LNM-R and LNM-F (50/50) were subcutaneously injected in 24, 36, 34, or 12 nude mice, respectively. (B) Typical immunohistochemistry for NTSR1 (left) or NTS (right) for tumors generated from LNM-R (top) or LNM-F (bottom) cells. Significant differences at *** P

    Article Snippet: These slides were then incubated with appropriate biotinylated secondary antibodies, NTS (Trekkie Biotinylates rabbit link, Biocare medical®), NTSR1 (Biotinylated anti-goat IgG, Vector laboratories, Inc), ErbB3 (Trekkie Biotinylates mouse link, Biocare medical®).

    Techniques: Generated, Mouse Assay, Injection, Immunohistochemistry

    Immunohistochemistry of NTSR1, lung cancer tumors (A) NTSR1 Immunolabeling in patients with primary lung adenocarcinomas (right) top X 50, bottom X400) and Squamous Cell carcinomas (left) . top X100, bottom X200. (B) Overall survival of patients operated for NSCLC lung adenocarcinoma according to NTSR1 score. Semiquantitative immunohistochemistry evaluation of NTSR1: NTSR1 + + +: strongly positive expression (number of staining cells > 50% and the labeling intensity is high = score 2), other: the remaining patients (score 0 and 1). left Survival curve for the first cohort, Center Survival curve for lung adenocarcinomas from the first cohort, right Survival curve for SCC and LCC from the first cohort (C) Overall survival of patients operated for lung adenocarcinoma according to NTSR1 score. Semiquantitative immunohistochemistry evaluation of NTSR1: NTSR1 + + +: strongly positive expression (number of staining cells > 50% and the labeling intensity is high = score 2), other: the remaining patients (score 0 and 1).

    Journal: Oncotarget

    Article Title: Neurotensin (NTS) and its receptor (NTSR1) causes EGFR, HER2 and HER3 over-expression and their autocrine/paracrine activation in lung tumors, confirming responsiveness to erlotinib

    doi:

    Figure Lengend Snippet: Immunohistochemistry of NTSR1, lung cancer tumors (A) NTSR1 Immunolabeling in patients with primary lung adenocarcinomas (right) top X 50, bottom X400) and Squamous Cell carcinomas (left) . top X100, bottom X200. (B) Overall survival of patients operated for NSCLC lung adenocarcinoma according to NTSR1 score. Semiquantitative immunohistochemistry evaluation of NTSR1: NTSR1 + + +: strongly positive expression (number of staining cells > 50% and the labeling intensity is high = score 2), other: the remaining patients (score 0 and 1). left Survival curve for the first cohort, Center Survival curve for lung adenocarcinomas from the first cohort, right Survival curve for SCC and LCC from the first cohort (C) Overall survival of patients operated for lung adenocarcinoma according to NTSR1 score. Semiquantitative immunohistochemistry evaluation of NTSR1: NTSR1 + + +: strongly positive expression (number of staining cells > 50% and the labeling intensity is high = score 2), other: the remaining patients (score 0 and 1).

    Article Snippet: These slides were then incubated with appropriate biotinylated secondary antibodies, NTS (Trekkie Biotinylates rabbit link, Biocare medical®), NTSR1 (Biotinylated anti-goat IgG, Vector laboratories, Inc), ErbB3 (Trekkie Biotinylates mouse link, Biocare medical®).

    Techniques: Immunohistochemistry, Immunolabeling, Expressing, Staining, Labeling

    NTS regulation enhanced HER2, and HER3 basal expression in human lung cancer cell lines (A) The mixture of cells R/F 20/80 lung cancer cells cultured for 72h, with the histograms representing intensity-based quantification of Western blot bands of basal total protein, EGFR, HER2, and HER3, Values are expressed as the percentage of the control LNM-F cells (which are the population more representative of the mixture) and are the mean ± SEM of 5 to 8 independent experiments. (B) An example of western blot gel of LNM-F, LNM-R and the mixture LNM-F, LNM-R (20/80) cultured for 72h no treated or treated with DMSO or 5x10 -6 M SR 48692. The blots were revealed with EGFR, HER2 or HER3 antibodies. The actin shown is to the protein control for the HER3 Blot (C) Lung cancer cells R-SI NTS treated or not with 10 -7 M JMV 449, DMSO or 5x10 -6 M SR 48692 for 48h. The histograms represent intensity-based quantification of Western blot bands of basal total protein, EGFR, HER2, and HER3. Values are expressed as the percentage of the non-treated cells (control), and are the mean ± SEM of 3 to 6 independent experiments.. Inset , An example of western blot gel of R-SI NTS cells treated with 10 -7 M JMV449, DMSO or 5x10 -6 M SR 48692 for 48h. Western blot bands of basal total EGFR, HER2, and HER3 protein. (D) EGFR, HER2, and HER3 immunolabeling in R-SI NTS cells treated of not with 10 -7 M JMV449 for 48 h. (E) Example of two restrictive areas from a patient with lung adenocarcinoma with a positives labeling for NTS, NTSR1, HER2, HER3.

    Journal: Oncotarget

    Article Title: Neurotensin (NTS) and its receptor (NTSR1) causes EGFR, HER2 and HER3 over-expression and their autocrine/paracrine activation in lung tumors, confirming responsiveness to erlotinib

    doi:

    Figure Lengend Snippet: NTS regulation enhanced HER2, and HER3 basal expression in human lung cancer cell lines (A) The mixture of cells R/F 20/80 lung cancer cells cultured for 72h, with the histograms representing intensity-based quantification of Western blot bands of basal total protein, EGFR, HER2, and HER3, Values are expressed as the percentage of the control LNM-F cells (which are the population more representative of the mixture) and are the mean ± SEM of 5 to 8 independent experiments. (B) An example of western blot gel of LNM-F, LNM-R and the mixture LNM-F, LNM-R (20/80) cultured for 72h no treated or treated with DMSO or 5x10 -6 M SR 48692. The blots were revealed with EGFR, HER2 or HER3 antibodies. The actin shown is to the protein control for the HER3 Blot (C) Lung cancer cells R-SI NTS treated or not with 10 -7 M JMV 449, DMSO or 5x10 -6 M SR 48692 for 48h. The histograms represent intensity-based quantification of Western blot bands of basal total protein, EGFR, HER2, and HER3. Values are expressed as the percentage of the non-treated cells (control), and are the mean ± SEM of 3 to 6 independent experiments.. Inset , An example of western blot gel of R-SI NTS cells treated with 10 -7 M JMV449, DMSO or 5x10 -6 M SR 48692 for 48h. Western blot bands of basal total EGFR, HER2, and HER3 protein. (D) EGFR, HER2, and HER3 immunolabeling in R-SI NTS cells treated of not with 10 -7 M JMV449 for 48 h. (E) Example of two restrictive areas from a patient with lung adenocarcinoma with a positives labeling for NTS, NTSR1, HER2, HER3.

    Article Snippet: These slides were then incubated with appropriate biotinylated secondary antibodies, NTS (Trekkie Biotinylates rabbit link, Biocare medical®), NTSR1 (Biotinylated anti-goat IgG, Vector laboratories, Inc), ErbB3 (Trekkie Biotinylates mouse link, Biocare medical®).

    Techniques: Expressing, Cell Culture, Western Blot, Immunolabeling, Labeling

    NTS/NTSR1 expressing tumors are the target for EGFR inhibitors treatment (A) LNM-R or R-SI NTSR1 cells (LNM-R expressing sh-RNA for NTSR1) were injected into the left and the right flank of the mice, respectively. Here is shown an example of a mouse from each group after 17 days of treatment. ( B and C ) Tumor growth generated by LNM-R cells (left flank) and R-SI NTSR1 cells (right flank) xenografted into nude mice and treated for 17 days with water, or 25 mg/kg erlotinib, or 200 mg/kg metformin, or both. At day one, 9 mice per group were randomized on LNM-R tumors size reaching approximately 20 mm 3 . (D) Tumor growth generated by R-SI NTSR1 cells xenografted into nude mice and treated for 24 days with water, or 25 mg/kg erlotinib, or 200 mg/kg metformin, or both. At day one, 10 mice per group were randomized on tumors size reaching approximately at 150 mm 3 .

    Journal: Oncotarget

    Article Title: Neurotensin (NTS) and its receptor (NTSR1) causes EGFR, HER2 and HER3 over-expression and their autocrine/paracrine activation in lung tumors, confirming responsiveness to erlotinib

    doi:

    Figure Lengend Snippet: NTS/NTSR1 expressing tumors are the target for EGFR inhibitors treatment (A) LNM-R or R-SI NTSR1 cells (LNM-R expressing sh-RNA for NTSR1) were injected into the left and the right flank of the mice, respectively. Here is shown an example of a mouse from each group after 17 days of treatment. ( B and C ) Tumor growth generated by LNM-R cells (left flank) and R-SI NTSR1 cells (right flank) xenografted into nude mice and treated for 17 days with water, or 25 mg/kg erlotinib, or 200 mg/kg metformin, or both. At day one, 9 mice per group were randomized on LNM-R tumors size reaching approximately 20 mm 3 . (D) Tumor growth generated by R-SI NTSR1 cells xenografted into nude mice and treated for 24 days with water, or 25 mg/kg erlotinib, or 200 mg/kg metformin, or both. At day one, 10 mice per group were randomized on tumors size reaching approximately at 150 mm 3 .

    Article Snippet: These slides were then incubated with appropriate biotinylated secondary antibodies, NTS (Trekkie Biotinylates rabbit link, Biocare medical®), NTSR1 (Biotinylated anti-goat IgG, Vector laboratories, Inc), ErbB3 (Trekkie Biotinylates mouse link, Biocare medical®).

    Techniques: Expressing, Injection, Mouse Assay, Generated

    NTS autocrine and paracrine regulation enhanced cellular growth in human lung cancer cell lines (A) Influence of NTS exogenous treatment on lung cancer cell growth. LNM-F, R-SI NTS and R-SI NTSR1 were grown in media containing 0 % FCS at low concentration and treated every day with 10 -8 M NTS or JMV 449 for 6 days. The ratio of the number of cells at Day 6/Day 0 was calculated. The result is expressed as the % of fold induction. Inset , NTS and NTSR1 transcripts analysis from a total of 200 ng of LNM-35, LNM-R, LNM-F, R-SI NTSR1 and R-SI NTS total RNA. (B) LNM-R and LNM-F were seeded alone or at the ratio of 20/80 LNM-R/LNM-F and grown in 0.1% FCS for 72h. The results are expressed as the ratio of the number of cells at 72h to T0 was calculated, and are the mean ± SEM of 7 independent experiments. C to F ) LNM-R and LNM-F were seeded alone or at the ratio of 20/80 LNM-R/LNM-F and grown in 0.1% FCS for 72h, The ratio of the number of cells at 72h to T0 was calculated. The results are expressed as the percentage of the growth induction compared to LNM-F. Results are the mean ± SEM of 2 to 5 independent experiments. Cells were exposed to (C) DMSO, 10 -7 M BIM 46174, 10 -6 M SR 48692, 1/200 rabbit IgG or anti NTS antibody. (D) DMSO, 10 -7 M M475271, 10 -5 M AG1478, PBS, or 50 μg/ml Herceptin. (E) 5×10 -6 M Gö6976, 10 -6 M U0126, 10 -6 M PD98059, or 10 -7 M LY294002. F) DMSO, 10 -5 M D-NAME, 10 -5 M L-NAME, 10 -5 M H7 or 5 10 -6 M Gö6976.

    Journal: Oncotarget

    Article Title: Neurotensin (NTS) and its receptor (NTSR1) causes EGFR, HER2 and HER3 over-expression and their autocrine/paracrine activation in lung tumors, confirming responsiveness to erlotinib

    doi:

    Figure Lengend Snippet: NTS autocrine and paracrine regulation enhanced cellular growth in human lung cancer cell lines (A) Influence of NTS exogenous treatment on lung cancer cell growth. LNM-F, R-SI NTS and R-SI NTSR1 were grown in media containing 0 % FCS at low concentration and treated every day with 10 -8 M NTS or JMV 449 for 6 days. The ratio of the number of cells at Day 6/Day 0 was calculated. The result is expressed as the % of fold induction. Inset , NTS and NTSR1 transcripts analysis from a total of 200 ng of LNM-35, LNM-R, LNM-F, R-SI NTSR1 and R-SI NTS total RNA. (B) LNM-R and LNM-F were seeded alone or at the ratio of 20/80 LNM-R/LNM-F and grown in 0.1% FCS for 72h. The results are expressed as the ratio of the number of cells at 72h to T0 was calculated, and are the mean ± SEM of 7 independent experiments. C to F ) LNM-R and LNM-F were seeded alone or at the ratio of 20/80 LNM-R/LNM-F and grown in 0.1% FCS for 72h, The ratio of the number of cells at 72h to T0 was calculated. The results are expressed as the percentage of the growth induction compared to LNM-F. Results are the mean ± SEM of 2 to 5 independent experiments. Cells were exposed to (C) DMSO, 10 -7 M BIM 46174, 10 -6 M SR 48692, 1/200 rabbit IgG or anti NTS antibody. (D) DMSO, 10 -7 M M475271, 10 -5 M AG1478, PBS, or 50 μg/ml Herceptin. (E) 5×10 -6 M Gö6976, 10 -6 M U0126, 10 -6 M PD98059, or 10 -7 M LY294002. F) DMSO, 10 -5 M D-NAME, 10 -5 M L-NAME, 10 -5 M H7 or 5 10 -6 M Gö6976.

    Article Snippet: These slides were then incubated with appropriate biotinylated secondary antibodies, NTS (Trekkie Biotinylates rabbit link, Biocare medical®), NTSR1 (Biotinylated anti-goat IgG, Vector laboratories, Inc), ErbB3 (Trekkie Biotinylates mouse link, Biocare medical®).

    Techniques: Concentration Assay