biotinylated anti avidin d  (Vector Laboratories)


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  • 97
    Name:
    Biotinylated Anti Avidin Antibody
    Description:
    Biotinylated Anti Avidin has been widely used as an amplifying reagent in immunohistochemistry in situ hybridization microarray assays ELISAs blots and many other applications The capability of binding avidin via either biotin binding sites or through antigen binding sites makes this biotinylated antibody unique Our antibodies to avidin are produced in goats using our highly purified avidin and isolated by affinity chromatography Anti Avidin does not bind streptavidin and Anti Streptavidin does not recognize avidin These antibodies provide opportunities to significantly amplify signals in many applications
    Catalog Number:
    ba-0300
    Price:
    None
    Host:
    Goat
    Size:
    0 5 mg
    Category:
    Antibodies
    Buy from Supplier


    Structured Review

    Vector Laboratories biotinylated anti avidin d
    Biotinylated Anti Avidin Antibody
    Biotinylated Anti Avidin has been widely used as an amplifying reagent in immunohistochemistry in situ hybridization microarray assays ELISAs blots and many other applications The capability of binding avidin via either biotin binding sites or through antigen binding sites makes this biotinylated antibody unique Our antibodies to avidin are produced in goats using our highly purified avidin and isolated by affinity chromatography Anti Avidin does not bind streptavidin and Anti Streptavidin does not recognize avidin These antibodies provide opportunities to significantly amplify signals in many applications
    https://www.bioz.com/result/biotinylated anti avidin d/product/Vector Laboratories
    Average 97 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    biotinylated anti avidin d - by Bioz Stars, 2020-07
    97/100 stars

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    Related Articles

    Incubation:

    Article Title: Novel Role for Matricellular Proteins in the Regulation of Islet β Cell Survival
    Article Snippet: .. For co-staining experiments, sections were incubated with SPARC goat antibody (1/50, R & D Systems) overnight at 4 °C, followed by incubation with biotinylated anti-goat antibody (Vector labs) for 1 h at room temperature. .. SPARC-positive areas were detected using the ABC and DAB peroxidase substrate kits (Vector labs).

    Article Title: Transforming single DNA molecules into fluorescent magnetic particles for detection and enumeration of genetic variations
    Article Snippet: .. They then were incubated for 10 min in a total volume of 100 μl of B-PCR buffer containing 6 μg of Alexa 488-conjugated chicken anti-rabbit antibody (no. A-21441, Molecular Probes) and 3 μg of biotinylated goat anti-avidin antibody (no. BA-0300, Vector Laboratories). ..

    Article Title: Modulation of inhibitory autapses and synapses on rat CA1 interneurones by GABAa receptor ligands
    Article Snippet: .. Sections were incubated overnight in ABC-peroxidase (Vector Laboratories, Peterborough, UK) and either processed to reveal peroxidase labelling with nickel-intensified DAB (diaminobenzidine)subsequently, or incubated for another 1 and 16 h in biotinylated goat anti-Avidin D (1:10000, Vector Laboratories), followed by 1 h in ABC peroxidase, to amplify the peroxidase labelling prior to DAB development. ..

    Amplification:

    Article Title: Effects of hepatitis B virus infection on human sperm chromosomes
    Article Snippet: .. Hybridization signals were detected with FITC-conjugated avidin and amplified with goat biotinylated anti-avidin antibody followed by another layer of FITC-avdin (avidin and anti-avidin, Vector Laboratories). .. To increase the intensity of the hybridization signal, a second round of amplification was applied by sandwich method as above.

    Article Title: Alternative lengthening of telomeres in normal mammalian somatic cells
    Article Snippet: .. The signal was amplified with biotinylated goat anti-avidin antibody (Vector Laboratories) diluted in 4× SSC, 1% BSA, and 0.1% Tween 20. .. Slides were counterstained with DAPI (Sigma) in 2× SSC for 5 min at room temperature and mounted with 2.3% DABCO (Sigma) in 90% glycerol.

    Avidin-Biotin Assay:

    Article Title: Effects of hepatitis B virus infection on human sperm chromosomes
    Article Snippet: .. Hybridization signals were detected with FITC-conjugated avidin and amplified with goat biotinylated anti-avidin antibody followed by another layer of FITC-avdin (avidin and anti-avidin, Vector Laboratories). .. To increase the intensity of the hybridization signal, a second round of amplification was applied by sandwich method as above.

    Article Title: Assessment of Gene Flow Between Gossypium hirsutum and G. herbaceum: Evidence of Unreduced Gametes in the Diploid Progenitor
    Article Snippet: .. Biotinylated probes were immunodetected by Texas-red-conjugated with avidin antibodies (Vector Laboratories, Burlingame, CA). .. The chromosomes were mounted and counterstained in Vectashield (Vector Laboratories) containing 2.5 µg/ml 4,6-diamidino-2-phenylindole (DAPI).

    Hybridization:

    Article Title: Effects of hepatitis B virus infection on human sperm chromosomes
    Article Snippet: .. Hybridization signals were detected with FITC-conjugated avidin and amplified with goat biotinylated anti-avidin antibody followed by another layer of FITC-avdin (avidin and anti-avidin, Vector Laboratories). .. To increase the intensity of the hybridization signal, a second round of amplification was applied by sandwich method as above.

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  • 99
    Vector Laboratories fluorescein anti avidin d
    Overexpression of GnT-V causes aberrant N-glycosylation in the transfected cells. (A) The level of GnT-V protein was detected in different cell lines by immunoblot. (B) The GnT-V-7721 or GnT-V-HT29 stable cells were identified by detecting the level of GnT-V protein using immunoblot. (C) GnT-V-7721 and Mock-7721 cells were seeded on coverslips, followed by fixation and staining with biotinylated L-PHA and FITC-conjugated avidin, and then visualized under fluorescence microscopy. (D) Flow cytometry was performed with biotinylated L-PHA in GnT-V-7721 and GnT-V-HT29 stable cells, as well as Mock cells. FITC-conjugated <t>avidin</t> D staining was used as negative control.
    Fluorescein Anti Avidin D, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein anti avidin d/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescein anti avidin d - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    85
    Vector Laboratories fluorescein isothiocyanate fitc conjugated horse anti mouse
    Overexpression of GnT-V causes aberrant N-glycosylation in the transfected cells. (A) The level of GnT-V protein was detected in different cell lines by immunoblot. (B) The GnT-V-7721 or GnT-V-HT29 stable cells were identified by detecting the level of GnT-V protein using immunoblot. (C) GnT-V-7721 and Mock-7721 cells were seeded on coverslips, followed by fixation and staining with biotinylated L-PHA and FITC-conjugated avidin, and then visualized under fluorescence microscopy. (D) Flow cytometry was performed with biotinylated L-PHA in GnT-V-7721 and GnT-V-HT29 stable cells, as well as Mock cells. FITC-conjugated <t>avidin</t> D staining was used as negative control.
    Fluorescein Isothiocyanate Fitc Conjugated Horse Anti Mouse, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein isothiocyanate fitc conjugated horse anti mouse/product/Vector Laboratories
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    fluorescein isothiocyanate fitc conjugated horse anti mouse - by Bioz Stars, 2020-07
    85/100 stars
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    99
    Vector Laboratories biotinylated goat anti mouse opg
    Overexpression of GnT-V causes aberrant N-glycosylation in the transfected cells. (A) The level of GnT-V protein was detected in different cell lines by immunoblot. (B) The GnT-V-7721 or GnT-V-HT29 stable cells were identified by detecting the level of GnT-V protein using immunoblot. (C) GnT-V-7721 and Mock-7721 cells were seeded on coverslips, followed by fixation and staining with biotinylated L-PHA and FITC-conjugated avidin, and then visualized under fluorescence microscopy. (D) Flow cytometry was performed with biotinylated L-PHA in GnT-V-7721 and GnT-V-HT29 stable cells, as well as Mock cells. FITC-conjugated <t>avidin</t> D staining was used as negative control.
    Biotinylated Goat Anti Mouse Opg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated goat anti mouse opg/product/Vector Laboratories
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated goat anti mouse opg - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    Overexpression of GnT-V causes aberrant N-glycosylation in the transfected cells. (A) The level of GnT-V protein was detected in different cell lines by immunoblot. (B) The GnT-V-7721 or GnT-V-HT29 stable cells were identified by detecting the level of GnT-V protein using immunoblot. (C) GnT-V-7721 and Mock-7721 cells were seeded on coverslips, followed by fixation and staining with biotinylated L-PHA and FITC-conjugated avidin, and then visualized under fluorescence microscopy. (D) Flow cytometry was performed with biotinylated L-PHA in GnT-V-7721 and GnT-V-HT29 stable cells, as well as Mock cells. FITC-conjugated avidin D staining was used as negative control.

    Journal: PLoS ONE

    Article Title: ?1,6 GlcNAc Branches-Modified PTPRT Attenuates Its Activity and Promotes Cell Migration by STAT3 Pathway

    doi: 10.1371/journal.pone.0098052

    Figure Lengend Snippet: Overexpression of GnT-V causes aberrant N-glycosylation in the transfected cells. (A) The level of GnT-V protein was detected in different cell lines by immunoblot. (B) The GnT-V-7721 or GnT-V-HT29 stable cells were identified by detecting the level of GnT-V protein using immunoblot. (C) GnT-V-7721 and Mock-7721 cells were seeded on coverslips, followed by fixation and staining with biotinylated L-PHA and FITC-conjugated avidin, and then visualized under fluorescence microscopy. (D) Flow cytometry was performed with biotinylated L-PHA in GnT-V-7721 and GnT-V-HT29 stable cells, as well as Mock cells. FITC-conjugated avidin D staining was used as negative control.

    Article Snippet: L-PHA Precipitation Biotinylated phaseolus vulgaris leucoagglutinin (L-PHA), biotinylated datura stramonium lectin (DSL), agarose bound L-PHA, agarose bound streptavidin and fluorescein anti-avidin D were purchased from Vector Laboratories (Burlingame, CA).

    Techniques: Over Expression, Transfection, Staining, Avidin-Biotin Assay, Fluorescence, Microscopy, Flow Cytometry, Cytometry, Negative Control