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biotin  (Agilent technologies)


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    Agilent technologies biotin
    Biotin, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    biotin - by Bioz Stars, 2025-06
    86/100 stars

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    Indicaxanthin prevents HFD-induced neuronal apoptosis. (A) TUNEL assay in the superficial cerebral cortex (microscope magnification 20×), showing blue (DAPI-stained nuclei) and red (TUNEL-positive cells) staining. Scale bars: 200 μm. (B) Number of apoptotic nuclei in the superficial cerebral cortex. (C, D) Results of semiquantitative PCR (C) and mRNA levels of the proapoptotic genes Fas-L , Bim , and P27 (D) in the mouse brain. (E, F) Representative image of the semiquantitative PCR results (E) and mRNA levels of the survival genes Bcl-2 and BDNF (F) in the mouse brain. Data are presented as the mean ± SEM ( n = 8/group). *** P < 0.001, vs . STD mice; # P < 0.05, ## P < 0.01; ### P < 0.001, vs . HFD-fed mice. BDNF: Brain-derived neurotrophic factor; HFD: high-fat diet; Ind: indicaxanthin; PCR: polymerase chain reaction; STD: standard diet; TUNEL: terminal <t>deoxynucleotidyl</t> <t>transferase</t> biotin-dUTP nick end labeling.
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    biotin  (Agilent technologies)
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    Indicaxanthin prevents HFD-induced neuronal apoptosis. (A) TUNEL assay in the superficial cerebral cortex (microscope magnification 20×), showing blue (DAPI-stained nuclei) and red (TUNEL-positive cells) staining. Scale bars: 200 μm. (B) Number of apoptotic nuclei in the superficial cerebral cortex. (C, D) Results of semiquantitative PCR (C) and mRNA levels of the proapoptotic genes Fas-L , Bim , and P27 (D) in the mouse brain. (E, F) Representative image of the semiquantitative PCR results (E) and mRNA levels of the survival genes Bcl-2 and BDNF (F) in the mouse brain. Data are presented as the mean ± SEM ( n = 8/group). *** P < 0.001, vs . STD mice; # P < 0.05, ## P < 0.01; ### P < 0.001, vs . HFD-fed mice. BDNF: Brain-derived neurotrophic factor; HFD: high-fat diet; Ind: indicaxanthin; PCR: polymerase chain reaction; STD: standard diet; TUNEL: terminal <t>deoxynucleotidyl</t> <t>transferase</t> biotin-dUTP nick end labeling.
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    Indicaxanthin prevents HFD-induced neuronal apoptosis. (A) TUNEL assay in the superficial cerebral cortex (microscope magnification 20×), showing blue (DAPI-stained nuclei) and red (TUNEL-positive cells) staining. Scale bars: 200 μm. (B) Number of apoptotic nuclei in the superficial cerebral cortex. (C, D) Results of semiquantitative PCR (C) and mRNA levels of the proapoptotic genes Fas-L , Bim , and P27 (D) in the mouse brain. (E, F) Representative image of the semiquantitative PCR results (E) and mRNA levels of the survival genes Bcl-2 and BDNF (F) in the mouse brain. Data are presented as the mean ± SEM ( n = 8/group). *** P < 0.001, vs . STD mice; # P < 0.05, ## P < 0.01; ### P < 0.001, vs . HFD-fed mice. BDNF: Brain-derived neurotrophic factor; HFD: high-fat diet; Ind: indicaxanthin; PCR: polymerase chain reaction; STD: standard diet; TUNEL: terminal <t>deoxynucleotidyl</t> <t>transferase</t> biotin-dUTP nick end labeling.
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    Indicaxanthin prevents HFD-induced neuronal apoptosis. (A) TUNEL assay in the superficial cerebral cortex (microscope magnification 20×), showing blue (DAPI-stained nuclei) and red (TUNEL-positive cells) staining. Scale bars: 200 μm. (B) Number of apoptotic nuclei in the superficial cerebral cortex. (C, D) Results of semiquantitative PCR (C) and mRNA levels of the proapoptotic genes Fas-L , Bim , and P27 (D) in the mouse brain. (E, F) Representative image of the semiquantitative PCR results (E) and mRNA levels of the survival genes Bcl-2 and BDNF (F) in the mouse brain. Data are presented as the mean ± SEM ( n = 8/group). *** P < 0.001, vs . STD mice; # P < 0.05, ## P < 0.01; ### P < 0.001, vs . HFD-fed mice. BDNF: Brain-derived neurotrophic factor; HFD: high-fat diet; Ind: indicaxanthin; PCR: polymerase chain reaction; STD: standard diet; TUNEL: terminal <t>deoxynucleotidyl</t> <t>transferase</t> biotin-dUTP nick end labeling.
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    Indicaxanthin prevents HFD-induced neuronal apoptosis. (A) TUNEL assay in the superficial cerebral cortex (microscope magnification 20×), showing blue (DAPI-stained nuclei) and red (TUNEL-positive cells) staining. Scale bars: 200 μm. (B) Number of apoptotic nuclei in the superficial cerebral cortex. (C, D) Results of semiquantitative PCR (C) and mRNA levels of the proapoptotic genes Fas-L , Bim , and P27 (D) in the mouse brain. (E, F) Representative image of the semiquantitative PCR results (E) and mRNA levels of the survival genes Bcl-2 and BDNF (F) in the mouse brain. Data are presented as the mean ± SEM ( n = 8/group). *** P < 0.001, vs . STD mice; # P < 0.05, ## P < 0.01; ### P < 0.001, vs . HFD-fed mice. BDNF: Brain-derived neurotrophic factor; HFD: high-fat diet; Ind: indicaxanthin; PCR: polymerase chain reaction; STD: standard diet; TUNEL: terminal <t>deoxynucleotidyl</t> <t>transferase</t> biotin-dUTP nick end labeling.
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    Indicaxanthin prevents HFD-induced neuronal apoptosis. (A) TUNEL assay in the superficial cerebral cortex (microscope magnification 20×), showing blue (DAPI-stained nuclei) and red (TUNEL-positive cells) staining. Scale bars: 200 μm. (B) Number of apoptotic nuclei in the superficial cerebral cortex. (C, D) Results of semiquantitative PCR (C) and mRNA levels of the proapoptotic genes Fas-L , Bim , and P27 (D) in the mouse brain. (E, F) Representative image of the semiquantitative PCR results (E) and mRNA levels of the survival genes Bcl-2 and BDNF (F) in the mouse brain. Data are presented as the mean ± SEM ( n = 8/group). *** P < 0.001, vs . STD mice; # P < 0.05, ## P < 0.01; ### P < 0.001, vs . HFD-fed mice. BDNF: Brain-derived neurotrophic factor; HFD: high-fat diet; Ind: indicaxanthin; PCR: polymerase chain reaction; STD: standard diet; TUNEL: terminal <t>deoxynucleotidyl</t> <t>transferase</t> biotin-dUTP nick end labeling.
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    Indicaxanthin prevents HFD-induced neuronal apoptosis. (A) TUNEL assay in the superficial cerebral cortex (microscope magnification 20×), showing blue (DAPI-stained nuclei) and red (TUNEL-positive cells) staining. Scale bars: 200 μm. (B) Number of apoptotic nuclei in the superficial cerebral cortex. (C, D) Results of semiquantitative PCR (C) and mRNA levels of the proapoptotic genes Fas-L , Bim , and P27 (D) in the mouse brain. (E, F) Representative image of the semiquantitative PCR results (E) and mRNA levels of the survival genes Bcl-2 and BDNF (F) in the mouse brain. Data are presented as the mean ± SEM ( n = 8/group). *** P < 0.001, vs . STD mice; # P < 0.05, ## P < 0.01; ### P < 0.001, vs . HFD-fed mice. BDNF: Brain-derived neurotrophic factor; HFD: high-fat diet; Ind: indicaxanthin; PCR: polymerase chain reaction; STD: standard diet; TUNEL: terminal <t>deoxynucleotidyl</t> <t>transferase</t> biotin-dUTP nick end labeling.
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    Indicaxanthin prevents HFD-induced neuronal apoptosis. (A) TUNEL assay in the superficial cerebral cortex (microscope magnification 20×), showing blue (DAPI-stained nuclei) and red (TUNEL-positive cells) staining. Scale bars: 200 μm. (B) Number of apoptotic nuclei in the superficial cerebral cortex. (C, D) Results of semiquantitative PCR (C) and mRNA levels of the proapoptotic genes Fas-L , Bim , and P27 (D) in the mouse brain. (E, F) Representative image of the semiquantitative PCR results (E) and mRNA levels of the survival genes Bcl-2 and BDNF (F) in the mouse brain. Data are presented as the mean ± SEM ( n = 8/group). *** P < 0.001, vs . STD mice; # P < 0.05, ## P < 0.01; ### P < 0.001, vs . HFD-fed mice. BDNF: Brain-derived neurotrophic factor; HFD: high-fat diet; Ind: indicaxanthin; PCR: polymerase chain reaction; STD: standard diet; TUNEL: terminal <t>deoxynucleotidyl</t> <t>transferase</t> biotin-dUTP nick end labeling.
    Avidin Biotin Blocking Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Indicaxanthin prevents HFD-induced neuronal apoptosis. (A) TUNEL assay in the superficial cerebral cortex (microscope magnification 20×), showing blue (DAPI-stained nuclei) and red (TUNEL-positive cells) staining. Scale bars: 200 μm. (B) Number of apoptotic nuclei in the superficial cerebral cortex. (C, D) Results of semiquantitative PCR (C) and mRNA levels of the proapoptotic genes Fas-L , Bim , and P27 (D) in the mouse brain. (E, F) Representative image of the semiquantitative PCR results (E) and mRNA levels of the survival genes Bcl-2 and BDNF (F) in the mouse brain. Data are presented as the mean ± SEM ( n = 8/group). *** P < 0.001, vs . STD mice; # P < 0.05, ## P < 0.01; ### P < 0.001, vs . HFD-fed mice. BDNF: Brain-derived neurotrophic factor; HFD: high-fat diet; Ind: indicaxanthin; PCR: polymerase chain reaction; STD: standard diet; TUNEL: terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling.

    Journal: Neural Regeneration Research

    Article Title: Positive impact of indicaxanthin from Opuntia ficus-indica fruit on high-fat diet–induced neuronal damage and gut microbiota dysbiosis

    doi: 10.4103/NRR.NRR-D-23-02039

    Figure Lengend Snippet: Indicaxanthin prevents HFD-induced neuronal apoptosis. (A) TUNEL assay in the superficial cerebral cortex (microscope magnification 20×), showing blue (DAPI-stained nuclei) and red (TUNEL-positive cells) staining. Scale bars: 200 μm. (B) Number of apoptotic nuclei in the superficial cerebral cortex. (C, D) Results of semiquantitative PCR (C) and mRNA levels of the proapoptotic genes Fas-L , Bim , and P27 (D) in the mouse brain. (E, F) Representative image of the semiquantitative PCR results (E) and mRNA levels of the survival genes Bcl-2 and BDNF (F) in the mouse brain. Data are presented as the mean ± SEM ( n = 8/group). *** P < 0.001, vs . STD mice; # P < 0.05, ## P < 0.01; ### P < 0.001, vs . HFD-fed mice. BDNF: Brain-derived neurotrophic factor; HFD: high-fat diet; Ind: indicaxanthin; PCR: polymerase chain reaction; STD: standard diet; TUNEL: terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling.

    Article Snippet: Thus, the halves of each brain were fixed in 4% formalin for 24 hours, followed by incubation with graded ethanol (50%, 70%, 85%, and 96%) for 5 minutes each, embedded in paraffin overnight and subsequently sectioned (5 μm thick) via an automatic microtome (Leica Biosystems, Buffalo Grove, IL, USA) (terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling [TUNEL] assay and immunofluorescence).

    Techniques: TUNEL Assay, Microscopy, Staining, Derivative Assay, Polymerase Chain Reaction, End Labeling

    Indicaxanthin mitigates brain oxidative damage induced by HFD. (A–D) Levels of cerebral MDA (A), RONS (B), and NO (C), and SOD-2 protein expression (D). (E) Densitometric analysis of SOD-2 protein levels normalized to β-actin levels. (F) Brain expression of cytosolic and nuclear Nrf-2. (G) Densitometric analysis of cytosolic Nrf-2 protein levels normalized to β-actin levels and nuclear Nrf2 normalized to laminin B levels. Data are presented as the mean ± SEM ( n = 8/group). *** P < 0.001, vs . STD mice; ### P < 0.001, vs . HFD-fed mice. HFD: High-fat diet; Ind: indicaxanthin; MDA: malondialdehyde; NO: nitric oxide; RONS: reactive oxygen and nitrogen species; SOD-2: enzyme superoxide dismutase-2; STD: standard diet; TUNEL: terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling.

    Journal: Neural Regeneration Research

    Article Title: Positive impact of indicaxanthin from Opuntia ficus-indica fruit on high-fat diet–induced neuronal damage and gut microbiota dysbiosis

    doi: 10.4103/NRR.NRR-D-23-02039

    Figure Lengend Snippet: Indicaxanthin mitigates brain oxidative damage induced by HFD. (A–D) Levels of cerebral MDA (A), RONS (B), and NO (C), and SOD-2 protein expression (D). (E) Densitometric analysis of SOD-2 protein levels normalized to β-actin levels. (F) Brain expression of cytosolic and nuclear Nrf-2. (G) Densitometric analysis of cytosolic Nrf-2 protein levels normalized to β-actin levels and nuclear Nrf2 normalized to laminin B levels. Data are presented as the mean ± SEM ( n = 8/group). *** P < 0.001, vs . STD mice; ### P < 0.001, vs . HFD-fed mice. HFD: High-fat diet; Ind: indicaxanthin; MDA: malondialdehyde; NO: nitric oxide; RONS: reactive oxygen and nitrogen species; SOD-2: enzyme superoxide dismutase-2; STD: standard diet; TUNEL: terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling.

    Article Snippet: Thus, the halves of each brain were fixed in 4% formalin for 24 hours, followed by incubation with graded ethanol (50%, 70%, 85%, and 96%) for 5 minutes each, embedded in paraffin overnight and subsequently sectioned (5 μm thick) via an automatic microtome (Leica Biosystems, Buffalo Grove, IL, USA) (terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling [TUNEL] assay and immunofluorescence).

    Techniques: Expressing, TUNEL Assay, End Labeling