biotin sp conjugated affinipure goat anti mouse  (Jackson Immuno)

 
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    Structured Review

    Jackson Immuno biotin sp conjugated affinipure goat anti mouse
    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated <t>AffiniPure</t> goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p
    Biotin Sp Conjugated Affinipure Goat Anti Mouse, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 84/100, based on 297 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis"

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0109352

    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p
    Figure Legend Snippet: Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p

    Techniques Used: Flow Cytometry, Cytometry, Cell Culture, Staining, Irradiation

    Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.
    Figure Legend Snippet: Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.

    Techniques Used: Immunofluorescence, Staining, Cell Culture

    2) Product Images from "Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis"

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0109352

    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p
    Figure Legend Snippet: Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p

    Techniques Used: Flow Cytometry, Cytometry, Cell Culture, Staining, Irradiation

    Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.
    Figure Legend Snippet: Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.

    Techniques Used: Immunofluorescence, Staining, Cell Culture

    3) Product Images from "Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis"

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0109352

    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p
    Figure Legend Snippet: Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p

    Techniques Used: Flow Cytometry, Cytometry, Cell Culture, Staining, Irradiation

    Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.
    Figure Legend Snippet: Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.

    Techniques Used: Immunofluorescence, Staining, Cell Culture

    4) Product Images from "Multiplex giant magnetoresistive biosensor microarrays identify interferon-associated autoantibodies in systemic lupus erythematosus"

    Article Title: Multiplex giant magnetoresistive biosensor microarrays identify interferon-associated autoantibodies in systemic lupus erythematosus

    Journal: Scientific Reports

    doi: 10.1038/srep27623

    GMR biosensor autoantigen microarrays. ( a ) Optical images of a GMR biosensor chip and a cartridge with a reaction well (left). The sensor chip measures 10 × 12 mm and consists of an array of 8 × 10 sensors (total 80 sensors). Each sensor size is 100 × 100 μm (right). ( b ) A schematic of assaying antibody reactivity to autoantigens (not to scale). (1) Autoantigens were printed on the surface of the chip’s sensors. (2) The sample was added to the reaction well, allowing antibodies to bind to their corresponding antigens. (3) After washing, species-specific, biotinylated anti-IgG antibodies were used as a secondary reagent. (4) Streptavidin-coated MNPs bind to the biotinylated detection antibodies, and the respective sensor detects stray field from the bound MNPs.
    Figure Legend Snippet: GMR biosensor autoantigen microarrays. ( a ) Optical images of a GMR biosensor chip and a cartridge with a reaction well (left). The sensor chip measures 10 × 12 mm and consists of an array of 8 × 10 sensors (total 80 sensors). Each sensor size is 100 × 100 μm (right). ( b ) A schematic of assaying antibody reactivity to autoantigens (not to scale). (1) Autoantigens were printed on the surface of the chip’s sensors. (2) The sample was added to the reaction well, allowing antibodies to bind to their corresponding antigens. (3) After washing, species-specific, biotinylated anti-IgG antibodies were used as a secondary reagent. (4) Streptavidin-coated MNPs bind to the biotinylated detection antibodies, and the respective sensor detects stray field from the bound MNPs.

    Techniques Used: Chromatin Immunoprecipitation

    5) Product Images from "Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis"

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0109352

    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p
    Figure Legend Snippet: Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p

    Techniques Used: Flow Cytometry, Cytometry, Cell Culture, Staining, Irradiation

    Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.
    Figure Legend Snippet: Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.

    Techniques Used: Immunofluorescence, Staining, Cell Culture

    6) Product Images from "T cell antigen discovery using soluble vaccinia proteome reveals recognition of antigens with both virion and non-virion association *"

    Article Title: T cell antigen discovery using soluble vaccinia proteome reveals recognition of antigens with both virion and non-virion association *

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1400663

    Immunogenicity of purified VACV-WR antigen, measured by antibody production and protection against VACV-WR challenge in mice (A) IgG subtype analysis. Antibodies were engendered in C57Bl/6 mice against nickel column-purified VACV IMV membrane protein, WR101/H3LΔTM that has been adjuvanted in CpG/ISCOMs or alum, or in PBS alone as a control. Sera were obtained after 14 days and probed against VACV proteome microarrays on to which 8 two-fold serial dilutions of purified WR101/H3L were printed. Specific reactivity to purified WR101/H3L was visualized using fluorescently-tagged secondary antibodies to IgG, IgG1 and IgG2c and signal intensities quantified in a confocal laser scanner; data for a single concentration of printed antigen is shown. (B) Relative proportions of IgG1 and IgG2c derived from data shown in (A). The IgG2a proportion of the total signal is shown above the zero line, and the IgG1 proportion shown below. The IgG response is polarized according to adjuvant. (C) Protection of B6 mice against intranasal (i.n.) challenge of VACV-WR using adjuvanted WR101/H3LΔTM and WR101/H3L. CpG/ISCOMs reduce weight loss and promote recovery compared to alum or PBS. (D) and (E) correlations between nadir body weight (expressed as percentage of original body weight) and titer of IgG2c and IgG1, respectively. Titer was defined from the WR101/H3L titration series on the array at the lowest concentration to give a signal intensity > 2000. Liner regression was used to generate the trend lines.
    Figure Legend Snippet: Immunogenicity of purified VACV-WR antigen, measured by antibody production and protection against VACV-WR challenge in mice (A) IgG subtype analysis. Antibodies were engendered in C57Bl/6 mice against nickel column-purified VACV IMV membrane protein, WR101/H3LΔTM that has been adjuvanted in CpG/ISCOMs or alum, or in PBS alone as a control. Sera were obtained after 14 days and probed against VACV proteome microarrays on to which 8 two-fold serial dilutions of purified WR101/H3L were printed. Specific reactivity to purified WR101/H3L was visualized using fluorescently-tagged secondary antibodies to IgG, IgG1 and IgG2c and signal intensities quantified in a confocal laser scanner; data for a single concentration of printed antigen is shown. (B) Relative proportions of IgG1 and IgG2c derived from data shown in (A). The IgG2a proportion of the total signal is shown above the zero line, and the IgG1 proportion shown below. The IgG response is polarized according to adjuvant. (C) Protection of B6 mice against intranasal (i.n.) challenge of VACV-WR using adjuvanted WR101/H3LΔTM and WR101/H3L. CpG/ISCOMs reduce weight loss and promote recovery compared to alum or PBS. (D) and (E) correlations between nadir body weight (expressed as percentage of original body weight) and titer of IgG2c and IgG1, respectively. Titer was defined from the WR101/H3L titration series on the array at the lowest concentration to give a signal intensity > 2000. Liner regression was used to generate the trend lines.

    Techniques Used: Purification, Mouse Assay, Nickel Column, Concentration Assay, Derivative Assay, Titration

    7) Product Images from "Obesity induces pro-inflammatory B cells and impairs B cell function in old mice"

    Article Title: Obesity induces pro-inflammatory B cells and impairs B cell function in old mice

    Journal: Mechanisms of ageing and development

    doi: 10.1016/j.mad.2017.01.004

    IgG2c is the major IgG subclass in the VAT. A. IgG subclasses in protein lysates of VAT lymphocytes were measured by Ig ELISA, after coating the plates with goat anti-mouse Southern Biotech purified anti-subclass antibodies at the concentration of 2 μg/ml. Detection antibody was a biotinylated goat anti-mouse IgG antibody, followed by streptavidin-HRP. Results are ratios of OD in subclasses/OD in total IgG. OD were measured with a spectrophotometer at 450 nm. B-C. ic staining of IgG2c in VAT ABC (B) and in splenic ABC (C) was performed after 48 h in culture in the absence (shadow, dotted line) or presence (solid line) of CpG. Mean Fluorescence Intensity from 3 independent experiments ± SE are shown in each quadrant. D-E. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and ABC percentages in VAT and spleen. Pearson's r = 0.85, p = 0.03 and r = 0.97, p = 0.002, respectively. F-G. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and FO percentages in VAT and spleen. Pearson's r = 0.09, p = 0.86 and r = −0.7, p = 0.13, respectively.
    Figure Legend Snippet: IgG2c is the major IgG subclass in the VAT. A. IgG subclasses in protein lysates of VAT lymphocytes were measured by Ig ELISA, after coating the plates with goat anti-mouse Southern Biotech purified anti-subclass antibodies at the concentration of 2 μg/ml. Detection antibody was a biotinylated goat anti-mouse IgG antibody, followed by streptavidin-HRP. Results are ratios of OD in subclasses/OD in total IgG. OD were measured with a spectrophotometer at 450 nm. B-C. ic staining of IgG2c in VAT ABC (B) and in splenic ABC (C) was performed after 48 h in culture in the absence (shadow, dotted line) or presence (solid line) of CpG. Mean Fluorescence Intensity from 3 independent experiments ± SE are shown in each quadrant. D-E. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and ABC percentages in VAT and spleen. Pearson's r = 0.85, p = 0.03 and r = 0.97, p = 0.002, respectively. F-G. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and FO percentages in VAT and spleen. Pearson's r = 0.09, p = 0.86 and r = −0.7, p = 0.13, respectively.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Purification, Concentration Assay, Spectrophotometry, Staining, Fluorescence

    8) Product Images from "Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis"

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0109352

    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p
    Figure Legend Snippet: Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p

    Techniques Used: Flow Cytometry, Cytometry, Cell Culture, Staining, Irradiation

    Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.
    Figure Legend Snippet: Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.

    Techniques Used: Immunofluorescence, Staining, Cell Culture

    9) Product Images from "The Bacterial Enzyme IdeS Cleaves the IgG-Type of B Cell Receptor (BCR), Abolishes BCR-Mediated Cell Signaling, and Inhibits Memory B Cell Activation"

    Article Title: The Bacterial Enzyme IdeS Cleaves the IgG-Type of B Cell Receptor (BCR), Abolishes BCR-Mediated Cell Signaling, and Inhibits Memory B Cell Activation

    Journal: The Journal of Immunology Author Choice

    doi: 10.4049/jimmunol.1501929

    IdeS cleaves IgG-type, but not IgM-type, of BCR on B cells. ( A ) Flow cytometry analysis of cells stained with biotinylated anti-Fab Ab, followed by streptavidin-allophycocyanin, after treatment of Nu-DUL-1 cells (IgG-type) and Daudi cells (IgM-type) with
    Figure Legend Snippet: IdeS cleaves IgG-type, but not IgM-type, of BCR on B cells. ( A ) Flow cytometry analysis of cells stained with biotinylated anti-Fab Ab, followed by streptavidin-allophycocyanin, after treatment of Nu-DUL-1 cells (IgG-type) and Daudi cells (IgM-type) with

    Techniques Used: Flow Cytometry, Cytometry, Staining

    10) Product Images from "The Bacterial Enzyme IdeS Cleaves the IgG-Type of B Cell Receptor (BCR), Abolishes BCR-Mediated Cell Signaling, and Inhibits Memory B Cell Activation"

    Article Title: The Bacterial Enzyme IdeS Cleaves the IgG-Type of B Cell Receptor (BCR), Abolishes BCR-Mediated Cell Signaling, and Inhibits Memory B Cell Activation

    Journal: The Journal of Immunology Author Choice

    doi: 10.4049/jimmunol.1501929

    IdeS cleaves IgG-type, but not IgM-type, of BCR on B cells. ( A ) Flow cytometry analysis of cells stained with biotinylated anti-Fab Ab, followed by streptavidin-allophycocyanin, after treatment of Nu-DUL-1 cells (IgG-type) and Daudi cells (IgM-type) with
    Figure Legend Snippet: IdeS cleaves IgG-type, but not IgM-type, of BCR on B cells. ( A ) Flow cytometry analysis of cells stained with biotinylated anti-Fab Ab, followed by streptavidin-allophycocyanin, after treatment of Nu-DUL-1 cells (IgG-type) and Daudi cells (IgM-type) with

    Techniques Used: Flow Cytometry, Cytometry, Staining

    11) Product Images from "The Bacterial Enzyme IdeS Cleaves the IgG-Type of B Cell Receptor (BCR), Abolishes BCR-Mediated Cell Signaling, and Inhibits Memory B Cell Activation"

    Article Title: The Bacterial Enzyme IdeS Cleaves the IgG-Type of B Cell Receptor (BCR), Abolishes BCR-Mediated Cell Signaling, and Inhibits Memory B Cell Activation

    Journal: The Journal of Immunology Author Choice

    doi: 10.4049/jimmunol.1501929

    IdeS cleaves IgG-type, but not IgM-type, of BCR on B cells. ( A ) Flow cytometry analysis of cells stained with biotinylated anti-Fab Ab, followed by streptavidin-allophycocyanin, after treatment of Nu-DUL-1 cells (IgG-type) and Daudi cells (IgM-type) with
    Figure Legend Snippet: IdeS cleaves IgG-type, but not IgM-type, of BCR on B cells. ( A ) Flow cytometry analysis of cells stained with biotinylated anti-Fab Ab, followed by streptavidin-allophycocyanin, after treatment of Nu-DUL-1 cells (IgG-type) and Daudi cells (IgM-type) with

    Techniques Used: Flow Cytometry, Cytometry, Staining

    Related Articles

    Transduction:

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis
    Article Snippet: Paragraph title: Plasmids, virus production, and gene transduction ... Biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072) and PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) were used for labeling the cells.

    Article Title: Overcoming Resistance of Human Non-Hodgkin’s Lymphoma to CD19-CAR CTL Therapy by Celecoxib and Histone Deacetylase Inhibitors
    Article Snippet: Paragraph title: 4.6. Measurement of Transduction Efficiency of CD19CAR Transduced CTLs ... Staining of the CD19 CAR construct was performed using a 1:5 dilution of Affinpure goat-anti mouse IgG (Jackson ImmunoResearch, West Grove, PA, USA, Cat# 115-065-072) diluted in FACS buffer.

    Flow Cytometry:

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis
    Article Snippet: After washing, the cells were stained with biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072), followed by PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) and CD56-FITC. .. The percentage of the NK cells that acquired anti-CD19-BB-ζ CARs through trogocytosis was determined by flow cytometry.

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis
    Article Snippet: The expression of anti-CD19-BB-ζ on the K562 cell surface was analyzed by flow cytometry on a FACSCalibur instrument using CellQuest software (BD Biosciences, San Jose, CA). .. Biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072) and PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) were used for labeling the cells.

    Article Title: Enhancing CAR T cell persistence through ICOS and 4-1BB costimulation
    Article Snippet: Paragraph title: Flow cytometry. ... Expression of CAR proteins was evaluated using biotinylated goat anti–mouse IgG (115-065-072, Jackson ImmunoResearch) with streptavidin (APC or PE) (BD Biosciences).

    Cytometry:

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis
    Article Snippet: After washing, the cells were stained with biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072), followed by PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) and CD56-FITC. .. The percentage of the NK cells that acquired anti-CD19-BB-ζ CARs through trogocytosis was determined by flow cytometry.

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis
    Article Snippet: The expression of anti-CD19-BB-ζ on the K562 cell surface was analyzed by flow cytometry on a FACSCalibur instrument using CellQuest software (BD Biosciences, San Jose, CA). .. Biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072) and PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) were used for labeling the cells.

    Article Title: Enhancing CAR T cell persistence through ICOS and 4-1BB costimulation
    Article Snippet: Paragraph title: Flow cytometry. ... Expression of CAR proteins was evaluated using biotinylated goat anti–mouse IgG (115-065-072, Jackson ImmunoResearch) with streptavidin (APC or PE) (BD Biosciences).

    Cell Culture:

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis
    Article Snippet: The NK cells were separated from the donor or control cells by density gradient centrifugation on Lymphoprep (Nycomed, Oslo, Norway) at 400×g for 20 min and were cultured in NK cell medium. .. After washing, the cells were stained with biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072), followed by PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) and CD56-FITC.

    Labeling:

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis
    Article Snippet: .. Biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072) and PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) were used for labeling the cells. .. Single K562-anti-CD19-BB-ζ cells with the highest expression of anti-CD19-BB-ζ were sorted with a FACSAria cell sorter (BD Biosciences, San Jose, CA).

    Incubation:

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis
    Article Snippet: After centrifugation at 1,400×g for 10 min, the tubes were incubated at 37°C for 4 h. After additional centrifugation and removal of the supernatant, K562 cells (5×104 ) were added to the tubes, and the tubes were incubated at 37°C for 24 h. This procedure was repeated for 7 more days. .. Biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072) and PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) were used for labeling the cells.

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis
    Article Snippet: .. The cells were then incubated with biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072) at 4°C overnight. .. After washing, the cells were incubated with Alexa Fluor 568-conjugated streptavidin (Invitrogen, Carlsbad, CA) and CD56-FITC antibodies (BD Biosciences, San Jose, CA).

    Article Title: Overcoming Resistance of Human Non-Hodgkin’s Lymphoma to CD19-CAR CTL Therapy by Celecoxib and Histone Deacetylase Inhibitors
    Article Snippet: Staining of the CD19 CAR construct was performed using a 1:5 dilution of Affinpure goat-anti mouse IgG (Jackson ImmunoResearch, West Grove, PA, USA, Cat# 115-065-072) diluted in FACS buffer. .. Cells were incubated for 30 min at 4 °C in this mixture, washed 2× with FACS buffer, and blocked for 20 min at 4 °C using a 1:10 dilution of mouse gamma globulin (Jackson ImmunoResearch, Cat# 015-000-002).

    Exclusion Assay:

    Article Title: Overcoming Resistance of Human Non-Hodgkin’s Lymphoma to CD19-CAR CTL Therapy by Celecoxib and Histone Deacetylase Inhibitors
    Article Snippet: An aliquot was used to assay for cell viability with the trypan blue dye exclusion assay. .. Staining of the CD19 CAR construct was performed using a 1:5 dilution of Affinpure goat-anti mouse IgG (Jackson ImmunoResearch, West Grove, PA, USA, Cat# 115-065-072) diluted in FACS buffer.

    Construct:

    Article Title: Overcoming Resistance of Human Non-Hodgkin’s Lymphoma to CD19-CAR CTL Therapy by Celecoxib and Histone Deacetylase Inhibitors
    Article Snippet: .. Staining of the CD19 CAR construct was performed using a 1:5 dilution of Affinpure goat-anti mouse IgG (Jackson ImmunoResearch, West Grove, PA, USA, Cat# 115-065-072) diluted in FACS buffer. .. Cells were incubated for 30 min at 4 °C in this mixture, washed 2× with FACS buffer, and blocked for 20 min at 4 °C using a 1:10 dilution of mouse gamma globulin (Jackson ImmunoResearch, Cat# 015-000-002).

    Expressing:

    Article Title: Pancreatic cancer therapy with combined mesothelin-redirected chimeric antigen receptor T cells and cytokine-armed oncolytic adenoviruses
    Article Snippet: .. Expression of meso-CAR on human T cells was detected with biotinylated goat anti–mouse IgG (specific for scFv of murine origin) (115-065-072; Jackson ImmunoResearch). .. Expression of mmeso-CAR on mouse T cells was detected with biotinylated goat anti–human IgG specific for scFv of human origin) (109-116-170; Jackson ImmunoResearch).

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis
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    Staining:

    Article Title: Pancreatic cancer therapy with combined mesothelin-redirected chimeric antigen receptor T cells and cytokine-armed oncolytic adenoviruses
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    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis
    Article Snippet: The cells were then incubated with biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072) at 4°C overnight. .. DAPI was used for staining the nucleus.

    Article Title: Enhancing CAR T cell persistence through ICOS and 4-1BB costimulation
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    FACS:

    Article Title: Enhancing CAR T cell persistence through ICOS and 4-1BB costimulation
    Article Snippet: For all experiments, T cell suspensions were stained with a fixable live/dead violet stain ( , Invitrogen) in PBS followed by surface antibody staining in FACS buffer. .. Expression of CAR proteins was evaluated using biotinylated goat anti–mouse IgG (115-065-072, Jackson ImmunoResearch) with streptavidin (APC or PE) (BD Biosciences).

    Article Title: Overcoming Resistance of Human Non-Hodgkin’s Lymphoma to CD19-CAR CTL Therapy by Celecoxib and Histone Deacetylase Inhibitors
    Article Snippet: .. Staining of the CD19 CAR construct was performed using a 1:5 dilution of Affinpure goat-anti mouse IgG (Jackson ImmunoResearch, West Grove, PA, USA, Cat# 115-065-072) diluted in FACS buffer. .. Cells were incubated for 30 min at 4 °C in this mixture, washed 2× with FACS buffer, and blocked for 20 min at 4 °C using a 1:10 dilution of mouse gamma globulin (Jackson ImmunoResearch, Cat# 015-000-002).

    Centrifugation:

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis
    Article Snippet: After centrifugation at 1,400×g for 10 min, the tubes were incubated at 37°C for 4 h. After additional centrifugation and removal of the supernatant, K562 cells (5×104 ) were added to the tubes, and the tubes were incubated at 37°C for 24 h. This procedure was repeated for 7 more days. .. Biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072) and PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) were used for labeling the cells.

    Gradient Centrifugation:

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis
    Article Snippet: The NK cells were separated from the donor or control cells by density gradient centrifugation on Lymphoprep (Nycomed, Oslo, Norway) at 400×g for 20 min and were cultured in NK cell medium. .. After washing, the cells were stained with biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072), followed by PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) and CD56-FITC.

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    Article Snippet: Paragraph title: Immunofluorescence analysis ... The cells were then incubated with biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072) at 4°C overnight.

    Blocking Assay:

    Article Title: Overcoming Resistance of Human Non-Hodgkin’s Lymphoma to CD19-CAR CTL Therapy by Celecoxib and Histone Deacetylase Inhibitors
    Article Snippet: Staining of the CD19 CAR construct was performed using a 1:5 dilution of Affinpure goat-anti mouse IgG (Jackson ImmunoResearch, West Grove, PA, USA, Cat# 115-065-072) diluted in FACS buffer. .. Excess blocking was washed with FACS buffer, and cells were stained with PE streptavidin (BD Biosciences, San Jose, CA, USA, Cat# 349023) (20 min, 4 °C).

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    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis
    Article Snippet: The expression of anti-CD19-BB-ζ on the K562 cell surface was analyzed by flow cytometry on a FACSCalibur instrument using CellQuest software (BD Biosciences, San Jose, CA). .. Biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072) and PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) were used for labeling the cells.

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    Jackson Immuno biotin sp conjugated affinipure goat anti mouse
    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated <t>AffiniPure</t> goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p
    Biotin Sp Conjugated Affinipure Goat Anti Mouse, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 84/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp conjugated affinipure goat anti mouse/product/Jackson Immuno
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    Jackson Immuno biotin sp affinipure goat anti mouse igg f ab 2 fragment specific
    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated <t>AffiniPure</t> goat anti-mouse <t>IgG,F(ab′)2</t> fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p
    Biotin Sp Affinipure Goat Anti Mouse Igg F Ab 2 Fragment Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno igm
    Plasma cells, B cells and macrophages in cardiac allografts with CAV (A) Left panel: Representative image of a tissue section from a CAV patient stained for CD20 + B cells (pink) and CD138 + plasma cells (brown). Middle and right panels: Immunofluorescence staining of consecutive formalin-fixed paraffin-embedded (FFPE) tissue sections from a representative CAV sample showing plasma cell–rich infiltrates adjacent to the coronary artery of a heart explant with CAV. CD138 + plasma cells (red) co-stained for secreted <t>IgM</t> or <t>IgG</t> (blue). Nuclei are shown in gray. (B) Representative staining of cardiac explant sections with CAV showing CD68 + macrophages (brown) and CD20 + B cells (pink, left panel) or CD138 + plasma cells (pink, middle panel). Right panel: Venn diagram representation of the type of infiltrates observed in the 56 CAV explants: B cell; plasma cell; and macrophage. The number of specimens with evidence of infiltrate is indicated for each cell type. One unique sample had no infiltration. (C) Left and middle left panel: Representative immunofluorescence staining of consecutive FFPE tissue sections of a cardiac explant with CAV for M2 macrophage marker CD163 (left) or M1 marker iNOS (middle left) in combination with CD68. Middle right panel: Immunofluorescence staining of an FFPE tissue section of a cardiac explant with CAV for M2 macrophage markers CD206 and CD163. Right panel: Representative immunofluorescence staining of an FFPE tissue section of a cardiac explant with CAV for IL-10 and M2 macrophage marker CD206.
    Igm, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p

    Journal: PLoS ONE

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis

    doi: 10.1371/journal.pone.0109352

    Figure Lengend Snippet: Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p

    Article Snippet: After washing, the cells were stained with biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072), followed by PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) and CD56-FITC.

    Techniques: Flow Cytometry, Cytometry, Cell Culture, Staining, Irradiation

    Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.

    Journal: PLoS ONE

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis

    doi: 10.1371/journal.pone.0109352

    Figure Lengend Snippet: Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.

    Article Snippet: After washing, the cells were stained with biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072), followed by PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) and CD56-FITC.

    Techniques: Immunofluorescence, Staining, Cell Culture

    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p

    Journal: PLoS ONE

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis

    doi: 10.1371/journal.pone.0109352

    Figure Lengend Snippet: Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p

    Article Snippet: Biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072) and PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) were used for labeling the cells.

    Techniques: Flow Cytometry, Cytometry, Cell Culture, Staining, Irradiation

    Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.

    Journal: PLoS ONE

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis

    doi: 10.1371/journal.pone.0109352

    Figure Lengend Snippet: Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.

    Article Snippet: Biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072) and PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) were used for labeling the cells.

    Techniques: Immunofluorescence, Staining, Cell Culture

    Plasma cells, B cells and macrophages in cardiac allografts with CAV (A) Left panel: Representative image of a tissue section from a CAV patient stained for CD20 + B cells (pink) and CD138 + plasma cells (brown). Middle and right panels: Immunofluorescence staining of consecutive formalin-fixed paraffin-embedded (FFPE) tissue sections from a representative CAV sample showing plasma cell–rich infiltrates adjacent to the coronary artery of a heart explant with CAV. CD138 + plasma cells (red) co-stained for secreted IgM or IgG (blue). Nuclei are shown in gray. (B) Representative staining of cardiac explant sections with CAV showing CD68 + macrophages (brown) and CD20 + B cells (pink, left panel) or CD138 + plasma cells (pink, middle panel). Right panel: Venn diagram representation of the type of infiltrates observed in the 56 CAV explants: B cell; plasma cell; and macrophage. The number of specimens with evidence of infiltrate is indicated for each cell type. One unique sample had no infiltration. (C) Left and middle left panel: Representative immunofluorescence staining of consecutive FFPE tissue sections of a cardiac explant with CAV for M2 macrophage marker CD163 (left) or M1 marker iNOS (middle left) in combination with CD68. Middle right panel: Immunofluorescence staining of an FFPE tissue section of a cardiac explant with CAV for M2 macrophage markers CD206 and CD163. Right panel: Representative immunofluorescence staining of an FFPE tissue section of a cardiac explant with CAV for IL-10 and M2 macrophage marker CD206.

    Journal: The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation

    Article Title: Prevalence of polyreactive innate clones among graft-infiltrating B cells in human cardiac allograft vasculopathy

    doi: 10.1016/j.healun.2017.09.011

    Figure Lengend Snippet: Plasma cells, B cells and macrophages in cardiac allografts with CAV (A) Left panel: Representative image of a tissue section from a CAV patient stained for CD20 + B cells (pink) and CD138 + plasma cells (brown). Middle and right panels: Immunofluorescence staining of consecutive formalin-fixed paraffin-embedded (FFPE) tissue sections from a representative CAV sample showing plasma cell–rich infiltrates adjacent to the coronary artery of a heart explant with CAV. CD138 + plasma cells (red) co-stained for secreted IgM or IgG (blue). Nuclei are shown in gray. (B) Representative staining of cardiac explant sections with CAV showing CD68 + macrophages (brown) and CD20 + B cells (pink, left panel) or CD138 + plasma cells (pink, middle panel). Right panel: Venn diagram representation of the type of infiltrates observed in the 56 CAV explants: B cell; plasma cell; and macrophage. The number of specimens with evidence of infiltrate is indicated for each cell type. One unique sample had no infiltration. (C) Left and middle left panel: Representative immunofluorescence staining of consecutive FFPE tissue sections of a cardiac explant with CAV for M2 macrophage marker CD163 (left) or M1 marker iNOS (middle left) in combination with CD68. Middle right panel: Immunofluorescence staining of an FFPE tissue section of a cardiac explant with CAV for M2 macrophage markers CD206 and CD163. Right panel: Representative immunofluorescence staining of an FFPE tissue section of a cardiac explant with CAV for IL-10 and M2 macrophage marker CD206.

    Article Snippet: Antibody binding was revealed with HRP-conjugated goat anti-human IgG or IgM (Jackson ImmunoResearch Laboratories, West Grove, PA), and developed using 3,3′,5,5′-tetramethylbenzidine (TMB; Life Technologies).

    Techniques: Staining, Immunofluorescence, Formalin-fixed Paraffin-Embedded, Marker