biotinylated anti goat  (Jackson Immuno)

 
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    Name:
    Biotin SP AffiniPure Donkey Anti Rabbit IgG
    Description:
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule rabbit IgG It also reacts with the light chains of other rabbit immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with bovine chicken goat guinea pig syrian hamster horse human mouse rat and sheep serum proteins but it may cross react with immunoglobulins from other species
    Catalog Number:
    711-065-152
    Price:
    115
    Purity:
    The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
    Conjugate:
    Biotin SP long spacer
    Size:
    ml
    Category:
    Secondary Antibody
    Source:
    Donkey
    Quantity:
    0 5
    Buy from Supplier


    Structured Review

    Jackson Immuno biotinylated anti goat
    Biotin SP AffiniPure Donkey Anti Rabbit IgG
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule rabbit IgG It also reacts with the light chains of other rabbit immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with bovine chicken goat guinea pig syrian hamster horse human mouse rat and sheep serum proteins but it may cross react with immunoglobulins from other species
    https://www.bioz.com/result/biotinylated anti goat/product/Jackson Immuno
    Average 90 stars, based on 291 article reviews
    Price from $9.99 to $1999.99
    biotinylated anti goat - by Bioz Stars, 2020-02
    90/100 stars

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    Related Articles

    Construct:

    Article Title: OREXIN 2 RECEPTOR REGULATION OF THE HYPOTHALAMIC–PITUITARY–ADRENAL (HPA) RESPONSE TO ACUTE AND REPEATED STRESS
    Article Snippet: Two additional groups of rats were injected with the DREADDs’ construct and exposed to either 1 or 5 days of restraint with injections of vehicle or CNO administered as previously described. .. Sections were incubated with c-Fos antibody (1:1250, sc-52; Santa Cruz Biotechnology, Santa Cruz, CA, USA) followed by Biotin-SP-conjugated AffiniPure Donkey-Antirabbit IgG (1:200, Jackson Immunoresearch, West Grove, PA, USA), which was visualized with a 3′3′-diaminobenzidine reaction (Sigma– Aldrich, St Louis, MO, USA).

    Incubation:

    Article Title: Palatable food avoidance and acceptance learning with different stressors in female rats
    Article Snippet: .. Briefly for the immunostaining, sections were incubated for 20 h with c-Fos primary antibody (1:10,000 dilution; PC38, Calbiochem) and 2 h with secondary donkey anti-rabbit (1:500 dilution; 711-065-152, Jackson Immuno Research). .. Then, sections were processed according to standard immunoperoxidase methods (ABC reagent, PK-6100, Vector Laboratories) and colorized with a commercially available peroxidase kit (SK-4100, Vector Laboratories).

    Article Title: Gene delivery to the spinal cord using MRI-guided focused ultrasound
    Article Snippet: .. Sections were washed 2 times with TBS for 15 min before a 2 h incubation with donkey anti-rabbit IgG biotin (1:500, Jackson ImmunoResearch, 711-065-152), diluted in blocking solution. .. After two 15 min washes in TBS, the sections were incubated with Cy3-conjugated Streptavidin (1:500, Jackson ImmunoResearch, 016-160-084) and Topro3 iodide (1:500, Invitrogen, T3605, Eugene, OR, USA) for 2 h at room temperature.

    Article Title: Phospho-regulation of ATOH1 Is Required for Plasticity of Secretory Progenitors and Tissue Regeneration
    Article Snippet: .. Slides were incubated with biotinylated donkey anti-rabbit secondary antibody (1:500, Jackson ImmunoResearch 711-065-152). .. Streptavidin-HRP conjugate (Vector Laboratories) was added onto the slides and incubated for 30 min. DAB Chromogen substrate (Dako) was added for dye development.

    Article Title: Lipopolysacharide Rapidly and Completely Suppresses AgRP Neuron-Mediated Food Intake in Male Mice
    Article Snippet: .. After several PBS washes, sections were incubated for 1 hour at room temperature in a biotinylated antirabbit (1:1000, 711-065-152; Jackson ImmunoResearch), a goat antichicken Alexa Fluor 488 (1:1000, ; Life Technologies), antirabbit Alexa Fluor 488 (1:1000, ; Life Technologies), or antigoat Alexa Fluor 594 (1:1000, A-11058; Life Technologies) secondary antibodies. .. After the biotinylated secondary antibodies, tissue was incubated with Streptavidin Alexa Fluor 488 (1:1000, ; Invitrogen) or in a solution of ABC (Vectastain Elite ABC kit; 1:1000).

    Article Title: OREXIN 2 RECEPTOR REGULATION OF THE HYPOTHALAMIC–PITUITARY–ADRENAL (HPA) RESPONSE TO ACUTE AND REPEATED STRESS
    Article Snippet: .. Sections were incubated with c-Fos antibody (1:1250, sc-52; Santa Cruz Biotechnology, Santa Cruz, CA, USA) followed by Biotin-SP-conjugated AffiniPure Donkey-Antirabbit IgG (1:200, Jackson Immunoresearch, West Grove, PA, USA), which was visualized with a 3′3′-diaminobenzidine reaction (Sigma– Aldrich, St Louis, MO, USA). .. Sections were then stained for orexin A (1:50, sc-8070; Santa Cruz Biotechnology, Santa Cruz, CA, USA) followed by biotinylated Horse Anti-Goat antibody (1:500, BA-9500; Vector Laboratories, Burlingame, CA, USA), which was visualized with a Nova Red reaction (SK-4800, Vector Laboratories, Burlingame, CA, USA).

    Article Title: Gene delivery to the spinal cord using MRI-guided focused ultrasound
    Article Snippet: .. This was followed by incubation at room temperature for 1 h in the following secondary antibodies in PBS++: Biotin-conjugated donkey anti-rabbit IgG (1:80 Jackson ImmunoResearch, 711-065-152, West Grove, PA, USA), Cy3-conjugated donkey anti-goat IgG (1:200, Jackson ImmunoResearch, 705-165-147), and Cy5-conjugated donkey anti-mouse IgG (1:200, Jackson ImmunoResearch, 715-175-150). .. After washing the sections twice for 10 min in PBS, they were incubated at room temperature in PBS++ with Alexa488-conjugated Streptavidin (1:200, Jackson ImmunoResearch, 016-540-084) for 2 h. After washing 3 times in PBS, sections were rinsed in 0.1M PO4 and mounted on a microscope slide with polyvinyl alcohol and 1,4-diazabicyclo[2.2.2]octane (PVA-DABCO) and a coverslip.

    Article Title: Neurokinin B Is Critical for Normal Timing of Sexual Maturation but Dispensable for Adult Reproductive Function in Female Mice
    Article Snippet: .. Tissue was then incubated in donkey antirabbit biotinylated secondary antibody (711–065-152; Jackson ImmunoResearch), followed by incubation in A/B solution (PK-6100; Vector Laboratories), and finally nickel-diaminobenzidine (Sk-4100; Vector Laboratories). .. Primary antibodies were as follows: kisspeptin (1:30,000, number 564, a generous gift of Dr Alain Caraty) , NKB (1:1000, NB300–201; Novus Biological), and GnRH (1:10,000, PA1–120; Thermo Scientific).

    Formalin-fixed Paraffin-Embedded:

    Article Title: Placental Underperfusion in a Rat Model of Intrauterine Growth Restriction Induced by a Reduced Plasma Volume Expansion
    Article Snippet: Immunohistochemistry (IHC) IHC analyses on formalin-fixed paraffin-embedded tissue sections (4μm) were carried out using the automated DiscoveryXT staining platform from Ventana Medical Systems (Tucson, AZ, USA). .. Biotin conjugated anti-rabbit secondary antibody from Jackson ImmunoResearch Labs (711-065-152; West Grove, PA, USA) was applied for 32min at RT and detected using the ChromoMap DAB detection kit (Ventana Medical Systems).

    Expressing:

    Article Title: A Novel Locus Harbouring a Functional CD164 Nonsense Mutation Identified in a Large Danish Family with Nonsyndromic Hearing Impairment
    Article Snippet: Paragraph title: CD164 expression in mouse inner ear ... The secondary antibody used was Jackson ImmunoResearch biotin-conjugated donkey anti-rabbit (711-065-152, 1:100).

    Article Title: Specific expression of Kcna10, Pxn and Odf2 in the organ of Corti
    Article Snippet: For each gene, slides covering the entire inner ear for three different mouse samples at each age were stained, and the observed expression patterns were only considered reliable if present in all three samples. .. Secondary antibodies used were Jackson ImmunoResearch (West Grove, PA, USA) biotin-conjugated donkey anti-rabbit (711-065-152) and Jackson ImmunoResearch biotin-conjugated donkey anti-goat (705-065-147).

    Article Title: Neurokinin B Is Critical for Normal Timing of Sexual Maturation but Dispensable for Adult Reproductive Function in Female Mice
    Article Snippet: Because ARH kisspeptin is thought to mediate sex steroid negative feedback in rodents , adult WT and Tac2 −/− female mice aged between 4 and 5 months of age underwent bilateral ovariectomy under isoflurane sedation to allow for maximum ARH kisspeptin expression. .. Tissue was then incubated in donkey antirabbit biotinylated secondary antibody (711–065-152; Jackson ImmunoResearch), followed by incubation in A/B solution (PK-6100; Vector Laboratories), and finally nickel-diaminobenzidine (Sk-4100; Vector Laboratories).

    Western Blot:

    Article Title: Axonal Injury Induces ATF3 in Specific Populations of Sacral Preganglionic Neurons in Male Rats
    Article Snippet: Western blot analysis of rat brain lysate detects a band at predicted weight, 46 kDa. .. Anti-rabbit biotin-SP (Jackson Immunoresearch; 711-065-152; batch 88458) 3.

    Electron Microscopy:

    Article Title: OREXIN 2 RECEPTOR REGULATION OF THE HYPOTHALAMIC–PITUITARY–ADRENAL (HPA) RESPONSE TO ACUTE AND REPEATED STRESS
    Article Snippet: Sections were incubated with c-Fos antibody (1:1250, sc-52; Santa Cruz Biotechnology, Santa Cruz, CA, USA) followed by Biotin-SP-conjugated AffiniPure Donkey-Antirabbit IgG (1:200, Jackson Immunoresearch, West Grove, PA, USA), which was visualized with a 3′3′-diaminobenzidine reaction (Sigma– Aldrich, St Louis, MO, USA). .. Sections were mounted with permount (Electron Microscopy Sciences, Fort Washington, PA, USA).

    Immunohistochemistry:

    Article Title: A Novel Locus Harbouring a Functional CD164 Nonsense Mutation Identified in a Large Danish Family with Nonsyndromic Hearing Impairment
    Article Snippet: Immunohistochemistry was then carried out according to the manufacturer’s instructions on slides using the Ventana Discovery machine with the manufacturer’s reagents CC1 (cat.no 950–124), EZPrep (cat.no 950–100), LCS (cat.no 650–010), RiboWash (cat.no 760–105), Reaction Buffer (cat.no 95–300), and RiboCC (cat.no 760–107). .. The secondary antibody used was Jackson ImmunoResearch biotin-conjugated donkey anti-rabbit (711-065-152, 1:100).

    Article Title: Specific expression of Kcna10, Pxn and Odf2 in the organ of Corti
    Article Snippet: Paragraph title: Immunohistochemistry ... Secondary antibodies used were Jackson ImmunoResearch (West Grove, PA, USA) biotin-conjugated donkey anti-rabbit (711-065-152) and Jackson ImmunoResearch biotin-conjugated donkey anti-goat (705-065-147).

    Article Title: Palatable food avoidance and acceptance learning with different stressors in female rats
    Article Snippet: Paragraph title: c-Fos immunohistochemistry ... Briefly for the immunostaining, sections were incubated for 20 h with c-Fos primary antibody (1:10,000 dilution; PC38, Calbiochem) and 2 h with secondary donkey anti-rabbit (1:500 dilution; 711-065-152, Jackson Immuno Research).

    Article Title: Gene delivery to the spinal cord using MRI-guided focused ultrasound
    Article Snippet: Paragraph title: Immunohistochemistry ... Sections were washed 2 times with TBS for 15 min before a 2 h incubation with donkey anti-rabbit IgG biotin (1:500, Jackson ImmunoResearch, 711-065-152), diluted in blocking solution.

    Article Title: Phospho-regulation of ATOH1 Is Required for Plasticity of Secretory Progenitors and Tissue Regeneration
    Article Snippet: Synaptophysin and Chromogranin A detection was carried out by manual IHC. .. Slides were incubated with biotinylated donkey anti-rabbit secondary antibody (1:500, Jackson ImmunoResearch 711-065-152).

    Article Title: Sepsis otopathy: experimental sepsis leads to significant hearing impairment due to apoptosis and glutamate excitotoxicity in murine cochlea
    Article Snippet: Paragraph title: Immunohistochemistry ... After incubating the sections with primary antibodies [cleaved caspase-3 1:400 (Asp175), Cell Signaling Technology, Danvers, MA; Bcl-2 (N-19), sc-492 1:200 Santa Cruz Biotechnology, Santa Cruz, CA; and Bax (P-19) rabbit polyclonal IgG492 1:50 Santa Cruz Biotechnology] for 1 hour at 37°C, a biotinylated donkey anti-rabbit IgG secondary antibody (1:400, Jackson ImmunoResearch 711-065-152) was applied for 30 minutes at room temperature.

    Article Title: Lipopolysacharide Rapidly and Completely Suppresses AgRP Neuron-Mediated Food Intake in Male Mice
    Article Snippet: Paragraph title: Immunohistochemistry (IHC) ... After several PBS washes, sections were incubated for 1 hour at room temperature in a biotinylated antirabbit (1:1000, 711-065-152; Jackson ImmunoResearch), a goat antichicken Alexa Fluor 488 (1:1000, ; Life Technologies), antirabbit Alexa Fluor 488 (1:1000, ; Life Technologies), or antigoat Alexa Fluor 594 (1:1000, A-11058; Life Technologies) secondary antibodies.

    Article Title: Nasal TRPA1 mediates irritant‐induced bradypnea in mice. Nasal TRPA1 mediates irritant‐induced bradypnea in mice
    Article Snippet: Paragraph title: Immunohistochemical analysis for activation of trigeminal nerve ... After cryoprotection with 30% sucrose in PBS, 16‐μ m‐thick serial longitudinal frozen sections were cut and every fourth section (10 slices/animal) was immunohistochemically stained for pERK (1/400, raised in rabbit, #4370S, Cell Signaling Technology) and a neuronal marker, NeuN (1/400, raised in guinea pig, #266004, Synaptic Systems). pERK was visualized with a biotinylated donkey anti‐rabbit IgG antibody (1/250, #711‐065‐152, Jackson Immunoresearch) and streptavidin‐conjugated Alexa Fluor 488 (1/200, S11223, Invitrogen).

    Article Title: Neurokinin B Is Critical for Normal Timing of Sexual Maturation but Dispensable for Adult Reproductive Function in Female Mice
    Article Snippet: Paragraph title: Immunohistochemistry (IHC) ... Tissue was then incubated in donkey antirabbit biotinylated secondary antibody (711–065-152; Jackson ImmunoResearch), followed by incubation in A/B solution (PK-6100; Vector Laboratories), and finally nickel-diaminobenzidine (Sk-4100; Vector Laboratories).

    Article Title: Placental Underperfusion in a Rat Model of Intrauterine Growth Restriction Induced by a Reduced Plasma Volume Expansion
    Article Snippet: Paragraph title: Immunohistochemistry (IHC) ... Biotin conjugated anti-rabbit secondary antibody from Jackson ImmunoResearch Labs (711-065-152; West Grove, PA, USA) was applied for 32min at RT and detected using the ChromoMap DAB detection kit (Ventana Medical Systems).

    Activation Assay:

    Article Title: Palatable food avoidance and acceptance learning with different stressors in female rats
    Article Snippet: To assess the neural activation produced by the four different US's, brains were frozen, and sectioned at 40 μm on a cryostat from the brainstem (for 5 series) through the forebrain (for 4 series). .. Briefly for the immunostaining, sections were incubated for 20 h with c-Fos primary antibody (1:10,000 dilution; PC38, Calbiochem) and 2 h with secondary donkey anti-rabbit (1:500 dilution; 711-065-152, Jackson Immuno Research).

    Article Title: OREXIN 2 RECEPTOR REGULATION OF THE HYPOTHALAMIC–PITUITARY–ADRENAL (HPA) RESPONSE TO ACUTE AND REPEATED STRESS
    Article Snippet: Paragraph title: Assaying orexin neuronal activation ... Sections were incubated with c-Fos antibody (1:1250, sc-52; Santa Cruz Biotechnology, Santa Cruz, CA, USA) followed by Biotin-SP-conjugated AffiniPure Donkey-Antirabbit IgG (1:200, Jackson Immunoresearch, West Grove, PA, USA), which was visualized with a 3′3′-diaminobenzidine reaction (Sigma– Aldrich, St Louis, MO, USA).

    Article Title: Nasal TRPA1 mediates irritant‐induced bradypnea in mice. Nasal TRPA1 mediates irritant‐induced bradypnea in mice
    Article Snippet: Paragraph title: Immunohistochemical analysis for activation of trigeminal nerve ... After cryoprotection with 30% sucrose in PBS, 16‐μ m‐thick serial longitudinal frozen sections were cut and every fourth section (10 slices/animal) was immunohistochemically stained for pERK (1/400, raised in rabbit, #4370S, Cell Signaling Technology) and a neuronal marker, NeuN (1/400, raised in guinea pig, #266004, Synaptic Systems). pERK was visualized with a biotinylated donkey anti‐rabbit IgG antibody (1/250, #711‐065‐152, Jackson Immunoresearch) and streptavidin‐conjugated Alexa Fluor 488 (1/200, S11223, Invitrogen).

    Northern Blot:

    Article Title: Fibroblast Growth Factor 9 Imparts Hierarchy and Vasoreactivity to the Microcirculation of Renal Tumors and Suppresses Metastases *
    Article Snippet: Unconjugated primary antibodies were reacted with biotinylated donkey anti-rabbit IgG (711-065-152, Jackson ImmunoResearch Laboratories) or horseradish peroxidase-conjugated donkey anti-goat IgG (sc-2020, Santa Cruz Biotechnology). .. Vessel density and coverage by mural cells were quantified using Northern Eclipse software.

    Labeling:

    Article Title: Nasal TRPA1 mediates irritant‐induced bradypnea in mice. Nasal TRPA1 mediates irritant‐induced bradypnea in mice
    Article Snippet: After cryoprotection with 30% sucrose in PBS, 16‐μ m‐thick serial longitudinal frozen sections were cut and every fourth section (10 slices/animal) was immunohistochemically stained for pERK (1/400, raised in rabbit, #4370S, Cell Signaling Technology) and a neuronal marker, NeuN (1/400, raised in guinea pig, #266004, Synaptic Systems). pERK was visualized with a biotinylated donkey anti‐rabbit IgG antibody (1/250, #711‐065‐152, Jackson Immunoresearch) and streptavidin‐conjugated Alexa Fluor 488 (1/200, S11223, Invitrogen). .. After cryoprotection with 30% sucrose in PBS, 16‐μ m‐thick serial longitudinal frozen sections were cut and every fourth section (10 slices/animal) was immunohistochemically stained for pERK (1/400, raised in rabbit, #4370S, Cell Signaling Technology) and a neuronal marker, NeuN (1/400, raised in guinea pig, #266004, Synaptic Systems). pERK was visualized with a biotinylated donkey anti‐rabbit IgG antibody (1/250, #711‐065‐152, Jackson Immunoresearch) and streptavidin‐conjugated Alexa Fluor 488 (1/200, S11223, Invitrogen).

    Generated:

    Article Title: Axonal Injury Induces ATF3 in Specific Populations of Sacral Preganglionic Neurons in Male Rats
    Article Snippet: SOM (Immunostar, Hudson, WI, United States; 20067; batch 8834021): polyclonal antibody generated in rabbit against synthetic somatostatin coupled to keyhole limpet hemocyanin with carbodiimide linker. .. Anti-rabbit biotin-SP (Jackson Immunoresearch; 711-065-152; batch 88458) 3.

    Imaging:

    Article Title: Palatable food avoidance and acceptance learning with different stressors in female rats
    Article Snippet: Briefly for the immunostaining, sections were incubated for 20 h with c-Fos primary antibody (1:10,000 dilution; PC38, Calbiochem) and 2 h with secondary donkey anti-rabbit (1:500 dilution; 711-065-152, Jackson Immuno Research). .. Quantitative analysis of c-Fos immunoreactivity was done using the IP Laboratory Imaging System (Scanalytics, Vienna, VA) image analysis software.

    Immunostaining:

    Article Title: Palatable food avoidance and acceptance learning with different stressors in female rats
    Article Snippet: .. Briefly for the immunostaining, sections were incubated for 20 h with c-Fos primary antibody (1:10,000 dilution; PC38, Calbiochem) and 2 h with secondary donkey anti-rabbit (1:500 dilution; 711-065-152, Jackson Immuno Research). .. Then, sections were processed according to standard immunoperoxidase methods (ABC reagent, PK-6100, Vector Laboratories) and colorized with a commercially available peroxidase kit (SK-4100, Vector Laboratories).

    Article Title: Fibroblast Growth Factor 9 Imparts Hierarchy and Vasoreactivity to the Microcirculation of Renal Tumors and Suppresses Metastases *
    Article Snippet: Paragraph title: Histology and Immunostaining ... Unconjugated primary antibodies were reacted with biotinylated donkey anti-rabbit IgG (711-065-152, Jackson ImmunoResearch Laboratories) or horseradish peroxidase-conjugated donkey anti-goat IgG (sc-2020, Santa Cruz Biotechnology).

    Immunofluorescence:

    Article Title: Phospho-regulation of ATOH1 Is Required for Plasticity of Secretory Progenitors and Tissue Regeneration
    Article Snippet: Slides were incubated with biotinylated donkey anti-rabbit secondary antibody (1:500, Jackson ImmunoResearch 711-065-152). .. For immunofluorescence tissue was excised and fixed for 48 h in 4% PFA in PBS at 4°C, after which it was transferred to 20% sucrose solution.

    Fluorescence:

    Article Title: Nasal TRPA1 mediates irritant‐induced bradypnea in mice. Nasal TRPA1 mediates irritant‐induced bradypnea in mice
    Article Snippet: After cryoprotection with 30% sucrose in PBS, 16‐μ m‐thick serial longitudinal frozen sections were cut and every fourth section (10 slices/animal) was immunohistochemically stained for pERK (1/400, raised in rabbit, #4370S, Cell Signaling Technology) and a neuronal marker, NeuN (1/400, raised in guinea pig, #266004, Synaptic Systems). pERK was visualized with a biotinylated donkey anti‐rabbit IgG antibody (1/250, #711‐065‐152, Jackson Immunoresearch) and streptavidin‐conjugated Alexa Fluor 488 (1/200, S11223, Invitrogen). .. After cryoprotection with 30% sucrose in PBS, 16‐μ m‐thick serial longitudinal frozen sections were cut and every fourth section (10 slices/animal) was immunohistochemically stained for pERK (1/400, raised in rabbit, #4370S, Cell Signaling Technology) and a neuronal marker, NeuN (1/400, raised in guinea pig, #266004, Synaptic Systems). pERK was visualized with a biotinylated donkey anti‐rabbit IgG antibody (1/250, #711‐065‐152, Jackson Immunoresearch) and streptavidin‐conjugated Alexa Fluor 488 (1/200, S11223, Invitrogen).

    Size-exclusion Chromatography:

    Article Title: Nasal TRPA1 mediates irritant‐induced bradypnea in mice. Nasal TRPA1 mediates irritant‐induced bradypnea in mice
    Article Snippet: For this purpose, urethane‐anesthetized WT, OB, and KO animals (which were different from those used in the respiratory measurement and not intubated) were exposed to the vapor of AITC or water for 60 sec in the nose (see Fig. B). .. After cryoprotection with 30% sucrose in PBS, 16‐μ m‐thick serial longitudinal frozen sections were cut and every fourth section (10 slices/animal) was immunohistochemically stained for pERK (1/400, raised in rabbit, #4370S, Cell Signaling Technology) and a neuronal marker, NeuN (1/400, raised in guinea pig, #266004, Synaptic Systems). pERK was visualized with a biotinylated donkey anti‐rabbit IgG antibody (1/250, #711‐065‐152, Jackson Immunoresearch) and streptavidin‐conjugated Alexa Fluor 488 (1/200, S11223, Invitrogen).

    Microscopy:

    Article Title: A Novel Locus Harbouring a Functional CD164 Nonsense Mutation Identified in a Large Danish Family with Nonsyndromic Hearing Impairment
    Article Snippet: The secondary antibody used was Jackson ImmunoResearch biotin-conjugated donkey anti-rabbit (711-065-152, 1:100). .. The stained slides were examined and images obtained using an AxioCam HRc camera mounted on a Zeiss microscope.

    Article Title: Gene delivery to the spinal cord using MRI-guided focused ultrasound
    Article Snippet: After washing the sections twice for 10 min in PBS, they were incubated at room temperature in PBS++ with Alexa488-conjugated Streptavidin (1:200, Jackson ImmunoResearch, 016-540-084) for 2 h. After washing 3 times in PBS, sections were rinsed in 0.1M PO4 and mounted on a microscope slide with polyvinyl alcohol and 1,4-diazabicyclo[2.2.2]octane (PVA-DABCO) and a coverslip. .. Sections were washed 2 times with TBS for 15 min before a 2 h incubation with donkey anti-rabbit IgG biotin (1:500, Jackson ImmunoResearch, 711-065-152), diluted in blocking solution.

    Article Title: OREXIN 2 RECEPTOR REGULATION OF THE HYPOTHALAMIC–PITUITARY–ADRENAL (HPA) RESPONSE TO ACUTE AND REPEATED STRESS
    Article Snippet: Sections were incubated with c-Fos antibody (1:1250, sc-52; Santa Cruz Biotechnology, Santa Cruz, CA, USA) followed by Biotin-SP-conjugated AffiniPure Donkey-Antirabbit IgG (1:200, Jackson Immunoresearch, West Grove, PA, USA), which was visualized with a 3′3′-diaminobenzidine reaction (Sigma– Aldrich, St Louis, MO, USA). .. Images were acquired with a Leitz DMR microscope with a digital camera (Leica Microsystems; Buffalo Grove, IL, USA).

    Article Title: Nasal TRPA1 mediates irritant‐induced bradypnea in mice. Nasal TRPA1 mediates irritant‐induced bradypnea in mice
    Article Snippet: After cryoprotection with 30% sucrose in PBS, 16‐μ m‐thick serial longitudinal frozen sections were cut and every fourth section (10 slices/animal) was immunohistochemically stained for pERK (1/400, raised in rabbit, #4370S, Cell Signaling Technology) and a neuronal marker, NeuN (1/400, raised in guinea pig, #266004, Synaptic Systems). pERK was visualized with a biotinylated donkey anti‐rabbit IgG antibody (1/250, #711‐065‐152, Jackson Immunoresearch) and streptavidin‐conjugated Alexa Fluor 488 (1/200, S11223, Invitrogen). .. After cryoprotection with 30% sucrose in PBS, 16‐μ m‐thick serial longitudinal frozen sections were cut and every fourth section (10 slices/animal) was immunohistochemically stained for pERK (1/400, raised in rabbit, #4370S, Cell Signaling Technology) and a neuronal marker, NeuN (1/400, raised in guinea pig, #266004, Synaptic Systems). pERK was visualized with a biotinylated donkey anti‐rabbit IgG antibody (1/250, #711‐065‐152, Jackson Immunoresearch) and streptavidin‐conjugated Alexa Fluor 488 (1/200, S11223, Invitrogen).

    Mouse Assay:

    Article Title: Neurokinin B Is Critical for Normal Timing of Sexual Maturation but Dispensable for Adult Reproductive Function in Female Mice
    Article Snippet: Because ARH kisspeptin is thought to mediate sex steroid negative feedback in rodents , adult WT and Tac2 −/− female mice aged between 4 and 5 months of age underwent bilateral ovariectomy under isoflurane sedation to allow for maximum ARH kisspeptin expression. .. Tissue was then incubated in donkey antirabbit biotinylated secondary antibody (711–065-152; Jackson ImmunoResearch), followed by incubation in A/B solution (PK-6100; Vector Laboratories), and finally nickel-diaminobenzidine (Sk-4100; Vector Laboratories).

    Blocking Assay:

    Article Title: Gene delivery to the spinal cord using MRI-guided focused ultrasound
    Article Snippet: .. Sections were washed 2 times with TBS for 15 min before a 2 h incubation with donkey anti-rabbit IgG biotin (1:500, Jackson ImmunoResearch, 711-065-152), diluted in blocking solution. .. After two 15 min washes in TBS, the sections were incubated with Cy3-conjugated Streptavidin (1:500, Jackson ImmunoResearch, 016-160-084) and Topro3 iodide (1:500, Invitrogen, T3605, Eugene, OR, USA) for 2 h at room temperature.

    Article Title: Gene delivery to the spinal cord using MRI-guided focused ultrasound
    Article Snippet: Immunohistochemistry Free-floating spinal cord sections were washed 3 times for 10 min in PBS, and incubated for 1 h in blocking solution containing PBS with 10% donkey serum and 0.4% Triton X100 (PBS++). .. This was followed by incubation at room temperature for 1 h in the following secondary antibodies in PBS++: Biotin-conjugated donkey anti-rabbit IgG (1:80 Jackson ImmunoResearch, 711-065-152, West Grove, PA, USA), Cy3-conjugated donkey anti-goat IgG (1:200, Jackson ImmunoResearch, 705-165-147), and Cy5-conjugated donkey anti-mouse IgG (1:200, Jackson ImmunoResearch, 715-175-150).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: A Novel Locus Harbouring a Functional CD164 Nonsense Mutation Identified in a Large Danish Family with Nonsyndromic Hearing Impairment
    Article Snippet: The DABMap Kit (Ventana; cat.no 760–124) with hematoxylin counterstain (cat.no 760–2021) and bluing reagent (cat.no 760–2037) were used. .. The secondary antibody used was Jackson ImmunoResearch biotin-conjugated donkey anti-rabbit (711-065-152, 1:100).

    Purification:

    Article Title: Axonal Injury Induces ATF3 in Specific Populations of Sacral Preganglionic Neurons in Male Rats
    Article Snippet: Whole antiserum is purified to provide an IgG fraction of antiserum. .. Anti-rabbit biotin-SP (Jackson Immunoresearch; 711-065-152; batch 88458) 3.

    Injection:

    Article Title: OREXIN 2 RECEPTOR REGULATION OF THE HYPOTHALAMIC–PITUITARY–ADRENAL (HPA) RESPONSE TO ACUTE AND REPEATED STRESS
    Article Snippet: Two additional groups of rats were injected with the DREADDs’ construct and exposed to either 1 or 5 days of restraint with injections of vehicle or CNO administered as previously described. .. Sections were incubated with c-Fos antibody (1:1250, sc-52; Santa Cruz Biotechnology, Santa Cruz, CA, USA) followed by Biotin-SP-conjugated AffiniPure Donkey-Antirabbit IgG (1:200, Jackson Immunoresearch, West Grove, PA, USA), which was visualized with a 3′3′-diaminobenzidine reaction (Sigma– Aldrich, St Louis, MO, USA).

    Plasmid Preparation:

    Article Title: From the Cover: Alterations in Optineurin Expression and Localization in Pre-clinical Parkinson’s Disease Models
    Article Snippet: Secondary antibodies Biotin-SP anti-rabbit (711-065-152), Alexa Fluor 647 anti-sheep (713-605-147), Alexa Fluor 488 anti-rabbit (711-545-152), Cy3 anti-mouse (715-165-151) were purchased from Jackson ImmunoResearch Laboratories, Inc (West Grove, Pennsylvania); IR 800CW anti-mouse (926-32212) was purchased from Licor (Lincoln, Nebraska). .. Secondary antibodies Biotin-SP anti-rabbit (711-065-152), Alexa Fluor 647 anti-sheep (713-605-147), Alexa Fluor 488 anti-rabbit (711-545-152), Cy3 anti-mouse (715-165-151) were purchased from Jackson ImmunoResearch Laboratories, Inc (West Grove, Pennsylvania); IR 800CW anti-mouse (926-32212) was purchased from Licor (Lincoln, Nebraska).

    Article Title: Palatable food avoidance and acceptance learning with different stressors in female rats
    Article Snippet: Briefly for the immunostaining, sections were incubated for 20 h with c-Fos primary antibody (1:10,000 dilution; PC38, Calbiochem) and 2 h with secondary donkey anti-rabbit (1:500 dilution; 711-065-152, Jackson Immuno Research). .. Briefly for the immunostaining, sections were incubated for 20 h with c-Fos primary antibody (1:10,000 dilution; PC38, Calbiochem) and 2 h with secondary donkey anti-rabbit (1:500 dilution; 711-065-152, Jackson Immuno Research).

    Article Title: Phospho-regulation of ATOH1 Is Required for Plasticity of Secretory Progenitors and Tissue Regeneration
    Article Snippet: Slides were incubated with biotinylated donkey anti-rabbit secondary antibody (1:500, Jackson ImmunoResearch 711-065-152). .. Streptavidin-HRP conjugate (Vector Laboratories) was added onto the slides and incubated for 30 min. DAB Chromogen substrate (Dako) was added for dye development.

    Article Title: Lipopolysacharide Rapidly and Completely Suppresses AgRP Neuron-Mediated Food Intake in Male Mice
    Article Snippet: After several PBS washes, sections were incubated for 1 hour at room temperature in a biotinylated antirabbit (1:1000, 711-065-152; Jackson ImmunoResearch), a goat antichicken Alexa Fluor 488 (1:1000, ; Life Technologies), antirabbit Alexa Fluor 488 (1:1000, ; Life Technologies), or antigoat Alexa Fluor 594 (1:1000, A-11058; Life Technologies) secondary antibodies. .. After several PBS washes, sections were incubated for 1 hour at room temperature in a biotinylated antirabbit (1:1000, 711-065-152; Jackson ImmunoResearch), a goat antichicken Alexa Fluor 488 (1:1000, ; Life Technologies), antirabbit Alexa Fluor 488 (1:1000, ; Life Technologies), or antigoat Alexa Fluor 594 (1:1000, A-11058; Life Technologies) secondary antibodies.

    Article Title: OREXIN 2 RECEPTOR REGULATION OF THE HYPOTHALAMIC–PITUITARY–ADRENAL (HPA) RESPONSE TO ACUTE AND REPEATED STRESS
    Article Snippet: Sections were incubated with c-Fos antibody (1:1250, sc-52; Santa Cruz Biotechnology, Santa Cruz, CA, USA) followed by Biotin-SP-conjugated AffiniPure Donkey-Antirabbit IgG (1:200, Jackson Immunoresearch, West Grove, PA, USA), which was visualized with a 3′3′-diaminobenzidine reaction (Sigma– Aldrich, St Louis, MO, USA). .. Sections were then stained for orexin A (1:50, sc-8070; Santa Cruz Biotechnology, Santa Cruz, CA, USA) followed by biotinylated Horse Anti-Goat antibody (1:500, BA-9500; Vector Laboratories, Burlingame, CA, USA), which was visualized with a Nova Red reaction (SK-4800, Vector Laboratories, Burlingame, CA, USA).

    Article Title: Fibroblast Growth Factor 9 Imparts Hierarchy and Vasoreactivity to the Microcirculation of Renal Tumors and Suppresses Metastases *
    Article Snippet: Unconjugated primary antibodies were reacted with biotinylated donkey anti-rabbit IgG (711-065-152, Jackson ImmunoResearch Laboratories) or horseradish peroxidase-conjugated donkey anti-goat IgG (sc-2020, Santa Cruz Biotechnology). .. Unconjugated primary antibodies were reacted with biotinylated donkey anti-rabbit IgG (711-065-152, Jackson ImmunoResearch Laboratories) or horseradish peroxidase-conjugated donkey anti-goat IgG (sc-2020, Santa Cruz Biotechnology).

    Software:

    Article Title: Palatable food avoidance and acceptance learning with different stressors in female rats
    Article Snippet: Briefly for the immunostaining, sections were incubated for 20 h with c-Fos primary antibody (1:10,000 dilution; PC38, Calbiochem) and 2 h with secondary donkey anti-rabbit (1:500 dilution; 711-065-152, Jackson Immuno Research). .. Quantitative analysis of c-Fos immunoreactivity was done using the IP Laboratory Imaging System (Scanalytics, Vienna, VA) image analysis software.

    Article Title: Placental Underperfusion in a Rat Model of Intrauterine Growth Restriction Induced by a Reduced Plasma Volume Expansion
    Article Snippet: Biotin conjugated anti-rabbit secondary antibody from Jackson ImmunoResearch Labs (711-065-152; West Grove, PA, USA) was applied for 32min at RT and detected using the ChromoMap DAB detection kit (Ventana Medical Systems). .. Quantitative image analysis was performed with the Visiomorph DP software (Visiopharm, Broomfield, CO, USA).

    In Situ:

    Article Title: Fibroblast Growth Factor 9 Imparts Hierarchy and Vasoreactivity to the Microcirculation of Renal Tumors and Suppresses Metastases *
    Article Snippet: Unconjugated primary antibodies were reacted with biotinylated donkey anti-rabbit IgG (711-065-152, Jackson ImmunoResearch Laboratories) or horseradish peroxidase-conjugated donkey anti-goat IgG (sc-2020, Santa Cruz Biotechnology). .. Apoptosis was detected using the In Situ Cell Death Detection Kit, Fluorescein (Roche Applied Science).

    Produced:

    Article Title: Palatable food avoidance and acceptance learning with different stressors in female rats
    Article Snippet: To assess the neural activation produced by the four different US's, brains were frozen, and sectioned at 40 μm on a cryostat from the brainstem (for 5 series) through the forebrain (for 4 series). .. Briefly for the immunostaining, sections were incubated for 20 h with c-Fos primary antibody (1:10,000 dilution; PC38, Calbiochem) and 2 h with secondary donkey anti-rabbit (1:500 dilution; 711-065-152, Jackson Immuno Research).

    Concentration Assay:

    Article Title: Neurokinin B Is Critical for Normal Timing of Sexual Maturation but Dispensable for Adult Reproductive Function in Female Mice
    Article Snippet: Tissue was then incubated in donkey antirabbit biotinylated secondary antibody (711–065-152; Jackson ImmunoResearch), followed by incubation in A/B solution (PK-6100; Vector Laboratories), and finally nickel-diaminobenzidine (Sk-4100; Vector Laboratories). .. The kisspeptin and GnRH antibodies have been previously characterized ( , ), and the NKB antibody showed no immunoreactivity when used at a concentration of 1:1000 in the brain of Tac2 −/− -deficient mice (Supplemental Figure 1).

    Cell Counting:

    Article Title: Neurokinin B Is Critical for Normal Timing of Sexual Maturation but Dispensable for Adult Reproductive Function in Female Mice
    Article Snippet: Tissue was then incubated in donkey antirabbit biotinylated secondary antibody (711–065-152; Jackson ImmunoResearch), followed by incubation in A/B solution (PK-6100; Vector Laboratories), and finally nickel-diaminobenzidine (Sk-4100; Vector Laboratories). .. For cell count and fiber density analysis, images were taken at a constant exposure with a total of 4 ARH and 2 anteroventral periventricular nucleus (AVPV) and 6 preoptic area sections analyzed per animal.

    Marker:

    Article Title: Nasal TRPA1 mediates irritant‐induced bradypnea in mice. Nasal TRPA1 mediates irritant‐induced bradypnea in mice
    Article Snippet: .. After cryoprotection with 30% sucrose in PBS, 16‐μ m‐thick serial longitudinal frozen sections were cut and every fourth section (10 slices/animal) was immunohistochemically stained for pERK (1/400, raised in rabbit, #4370S, Cell Signaling Technology) and a neuronal marker, NeuN (1/400, raised in guinea pig, #266004, Synaptic Systems). pERK was visualized with a biotinylated donkey anti‐rabbit IgG antibody (1/250, #711‐065‐152, Jackson Immunoresearch) and streptavidin‐conjugated Alexa Fluor 488 (1/200, S11223, Invitrogen). .. NeuN was visualized with a CF568‐conjugated anti‐guinea pig IgG antibody (1/200, raised in donkey, #20377, Biotium).

    Staining:

    Article Title: A Novel Locus Harbouring a Functional CD164 Nonsense Mutation Identified in a Large Danish Family with Nonsyndromic Hearing Impairment
    Article Snippet: All antibodies were diluted in ‘Antibody staining solution’: 10% fetal calf serum, 0.1% Triton, 2% BSA and 0.5% sodium azide in PBS. .. The secondary antibody used was Jackson ImmunoResearch biotin-conjugated donkey anti-rabbit (711-065-152, 1:100).

    Article Title: Specific expression of Kcna10, Pxn and Odf2 in the organ of Corti
    Article Snippet: For each gene, slides covering the entire inner ear for three different mouse samples at each age were stained, and the observed expression patterns were only considered reliable if present in all three samples. .. Secondary antibodies used were Jackson ImmunoResearch (West Grove, PA, USA) biotin-conjugated donkey anti-rabbit (711-065-152) and Jackson ImmunoResearch biotin-conjugated donkey anti-goat (705-065-147).

    Article Title: Palatable food avoidance and acceptance learning with different stressors in female rats
    Article Snippet: Briefly for the immunostaining, sections were incubated for 20 h with c-Fos primary antibody (1:10,000 dilution; PC38, Calbiochem) and 2 h with secondary donkey anti-rabbit (1:500 dilution; 711-065-152, Jackson Immuno Research). .. To control for staining variability, each immunohistochemistry run contained matched sections from all experimental groups and controls.

    Article Title: Sepsis otopathy: experimental sepsis leads to significant hearing impairment due to apoptosis and glutamate excitotoxicity in murine cochlea
    Article Snippet: After incubating the sections with primary antibodies [cleaved caspase-3 1:400 (Asp175), Cell Signaling Technology, Danvers, MA; Bcl-2 (N-19), sc-492 1:200 Santa Cruz Biotechnology, Santa Cruz, CA; and Bax (P-19) rabbit polyclonal IgG492 1:50 Santa Cruz Biotechnology] for 1 hour at 37°C, a biotinylated donkey anti-rabbit IgG secondary antibody (1:400, Jackson ImmunoResearch 711-065-152) was applied for 30 minutes at room temperature. .. The immunohistochemical staining reaction was referred to positive controls (tonsil) that were added to each experiment.

    Article Title: Lipopolysacharide Rapidly and Completely Suppresses AgRP Neuron-Mediated Food Intake in Male Mice
    Article Snippet: Moreover, staining in our samples displayed an intracellular distribution pattern consistent with aforementioned previous reports. .. After several PBS washes, sections were incubated for 1 hour at room temperature in a biotinylated antirabbit (1:1000, 711-065-152; Jackson ImmunoResearch), a goat antichicken Alexa Fluor 488 (1:1000, ; Life Technologies), antirabbit Alexa Fluor 488 (1:1000, ; Life Technologies), or antigoat Alexa Fluor 594 (1:1000, A-11058; Life Technologies) secondary antibodies.

    Article Title: OREXIN 2 RECEPTOR REGULATION OF THE HYPOTHALAMIC–PITUITARY–ADRENAL (HPA) RESPONSE TO ACUTE AND REPEATED STRESS
    Article Snippet: Specifically, sections of the lateral hypothalamus were cut and fixed as described above in DREADDs’ immunofluorescent staining. .. Sections were incubated with c-Fos antibody (1:1250, sc-52; Santa Cruz Biotechnology, Santa Cruz, CA, USA) followed by Biotin-SP-conjugated AffiniPure Donkey-Antirabbit IgG (1:200, Jackson Immunoresearch, West Grove, PA, USA), which was visualized with a 3′3′-diaminobenzidine reaction (Sigma– Aldrich, St Louis, MO, USA).

    Article Title: Nasal TRPA1 mediates irritant‐induced bradypnea in mice. Nasal TRPA1 mediates irritant‐induced bradypnea in mice
    Article Snippet: .. After cryoprotection with 30% sucrose in PBS, 16‐μ m‐thick serial longitudinal frozen sections were cut and every fourth section (10 slices/animal) was immunohistochemically stained for pERK (1/400, raised in rabbit, #4370S, Cell Signaling Technology) and a neuronal marker, NeuN (1/400, raised in guinea pig, #266004, Synaptic Systems). pERK was visualized with a biotinylated donkey anti‐rabbit IgG antibody (1/250, #711‐065‐152, Jackson Immunoresearch) and streptavidin‐conjugated Alexa Fluor 488 (1/200, S11223, Invitrogen). .. NeuN was visualized with a CF568‐conjugated anti‐guinea pig IgG antibody (1/200, raised in donkey, #20377, Biotium).

    Article Title: Placental Underperfusion in a Rat Model of Intrauterine Growth Restriction Induced by a Reduced Plasma Volume Expansion
    Article Snippet: Immunohistochemistry (IHC) IHC analyses on formalin-fixed paraffin-embedded tissue sections (4μm) were carried out using the automated DiscoveryXT staining platform from Ventana Medical Systems (Tucson, AZ, USA). .. Biotin conjugated anti-rabbit secondary antibody from Jackson ImmunoResearch Labs (711-065-152; West Grove, PA, USA) was applied for 32min at RT and detected using the ChromoMap DAB detection kit (Ventana Medical Systems).

    Article Title: Fibroblast Growth Factor 9 Imparts Hierarchy and Vasoreactivity to the Microcirculation of Renal Tumors and Suppresses Metastases *
    Article Snippet: Five-μm thick sections were stained with hematoxylin and eosin or were immunostained using biotinylated rat anti-mouse CD31 antibody (Clone MEC13.3, BD Biosciences), goat anti-VE-cadherin polyclonal antibody (AF1002, R & D Systems), mouse anti-smooth muscle α-actin antibody conjugated with alkaline phosphatase-conjugated (Clone 1A4, Sigma), rabbit anti-NG2 polyclonal antibody (AB5320, EMD Millipore), or rabbit anti-Ki-67 monoclonal antibody (ab16667, Abcam). .. Unconjugated primary antibodies were reacted with biotinylated donkey anti-rabbit IgG (711-065-152, Jackson ImmunoResearch Laboratories) or horseradish peroxidase-conjugated donkey anti-goat IgG (sc-2020, Santa Cruz Biotechnology).

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    Jackson Immuno cd106
    Candidate cell surface markers are differentially expressed in MB cell lines/primary cultures and patient samples A. Representative dot plots of staining for candidate biomarkers in Daoy, MED311-FH and UI226 tumorspheres (SHH variant) vs . tumorspheres from Group 3/Group 4 cell lines by flow cytometry. Insets: respective isotype controls. 7AAD: 7-aminoactinomycin D cell viability dye. B. - D. CD271 (B), CD171 (C) and <t>CD106</t> (D) expression in paraffin embedded sections of fetal cerebellum and primary medulloblastoma samples. Scale bar: 400 μm.
    Cd106, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd106/product/Jackson Immuno
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd106 - by Bioz Stars, 2020-02
    88/100 stars
      Buy from Supplier

    85
    Jackson Immuno biotin sp conjugated affinipure goat anti rabbit igg
    Candidate cell surface markers are differentially expressed in MB cell lines/primary cultures and patient samples A. Representative dot plots of staining for candidate biomarkers in Daoy, MED311-FH and UI226 tumorspheres (SHH variant) vs . tumorspheres from Group 3/Group 4 cell lines by flow cytometry. Insets: respective isotype controls. 7AAD: 7-aminoactinomycin D cell viability dye. B. - D. CD271 (B), CD171 (C) and <t>CD106</t> (D) expression in paraffin embedded sections of fetal cerebellum and primary medulloblastoma samples. Scale bar: 400 μm.
    Biotin Sp Conjugated Affinipure Goat Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp conjugated affinipure goat anti rabbit igg/product/Jackson Immuno
    Average 85 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    biotin sp conjugated affinipure goat anti rabbit igg - by Bioz Stars, 2020-02
    85/100 stars
      Buy from Supplier

    Image Search Results


    Candidate cell surface markers are differentially expressed in MB cell lines/primary cultures and patient samples A. Representative dot plots of staining for candidate biomarkers in Daoy, MED311-FH and UI226 tumorspheres (SHH variant) vs . tumorspheres from Group 3/Group 4 cell lines by flow cytometry. Insets: respective isotype controls. 7AAD: 7-aminoactinomycin D cell viability dye. B. - D. CD271 (B), CD171 (C) and CD106 (D) expression in paraffin embedded sections of fetal cerebellum and primary medulloblastoma samples. Scale bar: 400 μm.

    Journal: Oncotarget

    Article Title: Characterization of novel biomarkers in selecting for subtype specific medulloblastoma phenotypes

    doi:

    Figure Lengend Snippet: Candidate cell surface markers are differentially expressed in MB cell lines/primary cultures and patient samples A. Representative dot plots of staining for candidate biomarkers in Daoy, MED311-FH and UI226 tumorspheres (SHH variant) vs . tumorspheres from Group 3/Group 4 cell lines by flow cytometry. Insets: respective isotype controls. 7AAD: 7-aminoactinomycin D cell viability dye. B. - D. CD271 (B), CD171 (C) and CD106 (D) expression in paraffin embedded sections of fetal cerebellum and primary medulloblastoma samples. Scale bar: 400 μm.

    Article Snippet: Slides were washed in (CD271): 1XPBS, (CD171/CD106): 1XTBS and treated with secondary antibody: for CD271 (1:500), for CD106 (1:200) (Biotin-SP-Affinipure Goat Anti-Rabbit IgG (Jackson Immunoresearch, West Grove, PA, USA) and for CD171 (1:200) (Biotin-SP-AffiniPure Sheep Anti-Mouse IgG (H+L) (Jackson Immunoresearch) diluted in (CD271): 1% lamb serum in 1XPBS, (CD171/CD106): 1% goat serum / 1% BSA diluted in TBS for 2 hours at room temperature.

    Techniques: Staining, Variant Assay, Flow Cytometry, Cytometry, Expressing

    Transcript levels of CD271/p75NTR, CD171L1CAM, EGFR and CD106/VCAM1 across the 4 MB molecular variants Note that the CD271 Toronto dataset was previously published in Neoplasia, 15, Morrison et al., Deconstruction of Medulloblastoma cellular heterogeneity reveals differences between the most highly invasion and self-renewing phenotypes , 384-398, Copyright Elsevier (2013). A. - C. Gene expression data from 3 independent transcriptome datasets representing 548 patient samples showing downregulation of CD171/NCAM-L1 and relative enrichment of CD271/p75NTR, CD106/VCAM1, and EGFR, in SHH tumors compared with the other variants. Bars denote 1.5 interquartile range within each group. All subgroups were compared using a Kruskal-Wallis test for significance. Data are presented as log2 -transformed signal intensity. P

    Journal: Oncotarget

    Article Title: Characterization of novel biomarkers in selecting for subtype specific medulloblastoma phenotypes

    doi:

    Figure Lengend Snippet: Transcript levels of CD271/p75NTR, CD171L1CAM, EGFR and CD106/VCAM1 across the 4 MB molecular variants Note that the CD271 Toronto dataset was previously published in Neoplasia, 15, Morrison et al., Deconstruction of Medulloblastoma cellular heterogeneity reveals differences between the most highly invasion and self-renewing phenotypes , 384-398, Copyright Elsevier (2013). A. - C. Gene expression data from 3 independent transcriptome datasets representing 548 patient samples showing downregulation of CD171/NCAM-L1 and relative enrichment of CD271/p75NTR, CD106/VCAM1, and EGFR, in SHH tumors compared with the other variants. Bars denote 1.5 interquartile range within each group. All subgroups were compared using a Kruskal-Wallis test for significance. Data are presented as log2 -transformed signal intensity. P

    Article Snippet: Slides were washed in (CD271): 1XPBS, (CD171/CD106): 1XTBS and treated with secondary antibody: for CD271 (1:500), for CD106 (1:200) (Biotin-SP-Affinipure Goat Anti-Rabbit IgG (Jackson Immunoresearch, West Grove, PA, USA) and for CD171 (1:200) (Biotin-SP-AffiniPure Sheep Anti-Mouse IgG (H+L) (Jackson Immunoresearch) diluted in (CD271): 1% lamb serum in 1XPBS, (CD171/CD106): 1% goat serum / 1% BSA diluted in TBS for 2 hours at room temperature.

    Techniques: Expressing, Transformation Assay