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biotin x anxa5  (Thermo Fisher)


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    Thermo Fisher biotin x anxa5
    Annexin-containing EVs are shed from the repair scab after plasma membrane damage. (A) Confocal micrographs of ANXA2-mScarlet recruitment in three laser ablation experiments are shown. Image times are relative to the first image taken after ablation. White arrows in the top panes indicate the sites of ablation. Arrows in the bottom panes indicate EVs. Scale bars: 5 μm. (B) Representative confocal micrographs of ANXA2-mScarlet shedding are shown. Image times are relative to the first image taken after ablation. White arrows in panel indicate the site of ablation. Scale bars: 5 μm. (C) Representative confocal micrographs of ANXA2-mScarlet and ANXA1-mNeonGreen–expressing cells after laser ablation. Arrows indicate the ablation site. Scale bars: 10 μm. (D) Representative confocal micrographs of ANXA2-mScarlet and ANXA6-mNeonGreen–expressing cells after laser ablation. Arrows indicate the ablation site. Scale bars: 10 μm. (E) Representative widefield micrographs of cells stained with 1 μM Sytox Green after a treatment period with the indicated SLO concentration and recovery period. Scale bars: 150 μm. (F) Immunoblots show expression of annexin A2-Nluc (A2-Nluc) using a low expression promoter. (G) Immunoblots show enrichment of EV markers after capture with immobilized <t>annexin</t> <t>A5</t> from the conditioned medium 100k × g pellet fraction (FT—flow through; Elu—elution). (H) EV production index from ANXA2-Nluc cells expressing mCherry-VPS4a (dominant mutant) under control of a doxycycline-inducible promoter. Cells were pretreated with 200 ng/ml doxycycline (Dox) or DMSO for 6 h, followed by treatment with 200 ng/ml SLO. Error bars indicate three experimental replicates. Source data are available for this figure: .
    Biotin X Anxa5, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin x anxa5/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    biotin x anxa5 - by Bioz Stars, 2025-06
    86/100 stars

    Images

    1) Product Images from "Calpains orchestrate secretion of annexin-containing microvesicles during membrane repair"

    Article Title: Calpains orchestrate secretion of annexin-containing microvesicles during membrane repair

    Journal: The Journal of Cell Biology

    doi: 10.1083/jcb.202408159

    Annexin-containing EVs are shed from the repair scab after plasma membrane damage. (A) Confocal micrographs of ANXA2-mScarlet recruitment in three laser ablation experiments are shown. Image times are relative to the first image taken after ablation. White arrows in the top panes indicate the sites of ablation. Arrows in the bottom panes indicate EVs. Scale bars: 5 μm. (B) Representative confocal micrographs of ANXA2-mScarlet shedding are shown. Image times are relative to the first image taken after ablation. White arrows in panel indicate the site of ablation. Scale bars: 5 μm. (C) Representative confocal micrographs of ANXA2-mScarlet and ANXA1-mNeonGreen–expressing cells after laser ablation. Arrows indicate the ablation site. Scale bars: 10 μm. (D) Representative confocal micrographs of ANXA2-mScarlet and ANXA6-mNeonGreen–expressing cells after laser ablation. Arrows indicate the ablation site. Scale bars: 10 μm. (E) Representative widefield micrographs of cells stained with 1 μM Sytox Green after a treatment period with the indicated SLO concentration and recovery period. Scale bars: 150 μm. (F) Immunoblots show expression of annexin A2-Nluc (A2-Nluc) using a low expression promoter. (G) Immunoblots show enrichment of EV markers after capture with immobilized annexin A5 from the conditioned medium 100k × g pellet fraction (FT—flow through; Elu—elution). (H) EV production index from ANXA2-Nluc cells expressing mCherry-VPS4a (dominant mutant) under control of a doxycycline-inducible promoter. Cells were pretreated with 200 ng/ml doxycycline (Dox) or DMSO for 6 h, followed by treatment with 200 ng/ml SLO. Error bars indicate three experimental replicates. Source data are available for this figure: .
    Figure Legend Snippet: Annexin-containing EVs are shed from the repair scab after plasma membrane damage. (A) Confocal micrographs of ANXA2-mScarlet recruitment in three laser ablation experiments are shown. Image times are relative to the first image taken after ablation. White arrows in the top panes indicate the sites of ablation. Arrows in the bottom panes indicate EVs. Scale bars: 5 μm. (B) Representative confocal micrographs of ANXA2-mScarlet shedding are shown. Image times are relative to the first image taken after ablation. White arrows in panel indicate the site of ablation. Scale bars: 5 μm. (C) Representative confocal micrographs of ANXA2-mScarlet and ANXA1-mNeonGreen–expressing cells after laser ablation. Arrows indicate the ablation site. Scale bars: 10 μm. (D) Representative confocal micrographs of ANXA2-mScarlet and ANXA6-mNeonGreen–expressing cells after laser ablation. Arrows indicate the ablation site. Scale bars: 10 μm. (E) Representative widefield micrographs of cells stained with 1 μM Sytox Green after a treatment period with the indicated SLO concentration and recovery period. Scale bars: 150 μm. (F) Immunoblots show expression of annexin A2-Nluc (A2-Nluc) using a low expression promoter. (G) Immunoblots show enrichment of EV markers after capture with immobilized annexin A5 from the conditioned medium 100k × g pellet fraction (FT—flow through; Elu—elution). (H) EV production index from ANXA2-Nluc cells expressing mCherry-VPS4a (dominant mutant) under control of a doxycycline-inducible promoter. Cells were pretreated with 200 ng/ml doxycycline (Dox) or DMSO for 6 h, followed by treatment with 200 ng/ml SLO. Error bars indicate three experimental replicates. Source data are available for this figure: .

    Techniques Used: Clinical Proteomics, Membrane, Expressing, Staining, Concentration Assay, Western Blot, Mutagenesis, Control



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    biotin x anxa5  (Thermo Fisher)
    86
    Thermo Fisher biotin x anxa5
    Annexin-containing EVs are shed from the repair scab after plasma membrane damage. (A) Confocal micrographs of ANXA2-mScarlet recruitment in three laser ablation experiments are shown. Image times are relative to the first image taken after ablation. White arrows in the top panes indicate the sites of ablation. Arrows in the bottom panes indicate EVs. Scale bars: 5 μm. (B) Representative confocal micrographs of ANXA2-mScarlet shedding are shown. Image times are relative to the first image taken after ablation. White arrows in panel indicate the site of ablation. Scale bars: 5 μm. (C) Representative confocal micrographs of ANXA2-mScarlet and ANXA1-mNeonGreen–expressing cells after laser ablation. Arrows indicate the ablation site. Scale bars: 10 μm. (D) Representative confocal micrographs of ANXA2-mScarlet and ANXA6-mNeonGreen–expressing cells after laser ablation. Arrows indicate the ablation site. Scale bars: 10 μm. (E) Representative widefield micrographs of cells stained with 1 μM Sytox Green after a treatment period with the indicated SLO concentration and recovery period. Scale bars: 150 μm. (F) Immunoblots show expression of annexin A2-Nluc (A2-Nluc) using a low expression promoter. (G) Immunoblots show enrichment of EV markers after capture with immobilized <t>annexin</t> <t>A5</t> from the conditioned medium 100k × g pellet fraction (FT—flow through; Elu—elution). (H) EV production index from ANXA2-Nluc cells expressing mCherry-VPS4a (dominant mutant) under control of a doxycycline-inducible promoter. Cells were pretreated with 200 ng/ml doxycycline (Dox) or DMSO for 6 h, followed by treatment with 200 ng/ml SLO. Error bars indicate three experimental replicates. Source data are available for this figure: .
    Biotin X Anxa5, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin x anxa5/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    biotin x anxa5 - by Bioz Stars, 2025-06
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Annexin-containing EVs are shed from the repair scab after plasma membrane damage. (A) Confocal micrographs of ANXA2-mScarlet recruitment in three laser ablation experiments are shown. Image times are relative to the first image taken after ablation. White arrows in the top panes indicate the sites of ablation. Arrows in the bottom panes indicate EVs. Scale bars: 5 μm. (B) Representative confocal micrographs of ANXA2-mScarlet shedding are shown. Image times are relative to the first image taken after ablation. White arrows in panel indicate the site of ablation. Scale bars: 5 μm. (C) Representative confocal micrographs of ANXA2-mScarlet and ANXA1-mNeonGreen–expressing cells after laser ablation. Arrows indicate the ablation site. Scale bars: 10 μm. (D) Representative confocal micrographs of ANXA2-mScarlet and ANXA6-mNeonGreen–expressing cells after laser ablation. Arrows indicate the ablation site. Scale bars: 10 μm. (E) Representative widefield micrographs of cells stained with 1 μM Sytox Green after a treatment period with the indicated SLO concentration and recovery period. Scale bars: 150 μm. (F) Immunoblots show expression of annexin A2-Nluc (A2-Nluc) using a low expression promoter. (G) Immunoblots show enrichment of EV markers after capture with immobilized annexin A5 from the conditioned medium 100k × g pellet fraction (FT—flow through; Elu—elution). (H) EV production index from ANXA2-Nluc cells expressing mCherry-VPS4a (dominant mutant) under control of a doxycycline-inducible promoter. Cells were pretreated with 200 ng/ml doxycycline (Dox) or DMSO for 6 h, followed by treatment with 200 ng/ml SLO. Error bars indicate three experimental replicates. Source data are available for this figure: .

    Journal: The Journal of Cell Biology

    Article Title: Calpains orchestrate secretion of annexin-containing microvesicles during membrane repair

    doi: 10.1083/jcb.202408159

    Figure Lengend Snippet: Annexin-containing EVs are shed from the repair scab after plasma membrane damage. (A) Confocal micrographs of ANXA2-mScarlet recruitment in three laser ablation experiments are shown. Image times are relative to the first image taken after ablation. White arrows in the top panes indicate the sites of ablation. Arrows in the bottom panes indicate EVs. Scale bars: 5 μm. (B) Representative confocal micrographs of ANXA2-mScarlet shedding are shown. Image times are relative to the first image taken after ablation. White arrows in panel indicate the site of ablation. Scale bars: 5 μm. (C) Representative confocal micrographs of ANXA2-mScarlet and ANXA1-mNeonGreen–expressing cells after laser ablation. Arrows indicate the ablation site. Scale bars: 10 μm. (D) Representative confocal micrographs of ANXA2-mScarlet and ANXA6-mNeonGreen–expressing cells after laser ablation. Arrows indicate the ablation site. Scale bars: 10 μm. (E) Representative widefield micrographs of cells stained with 1 μM Sytox Green after a treatment period with the indicated SLO concentration and recovery period. Scale bars: 150 μm. (F) Immunoblots show expression of annexin A2-Nluc (A2-Nluc) using a low expression promoter. (G) Immunoblots show enrichment of EV markers after capture with immobilized annexin A5 from the conditioned medium 100k × g pellet fraction (FT—flow through; Elu—elution). (H) EV production index from ANXA2-Nluc cells expressing mCherry-VPS4a (dominant mutant) under control of a doxycycline-inducible promoter. Cells were pretreated with 200 ng/ml doxycycline (Dox) or DMSO for 6 h, followed by treatment with 200 ng/ml SLO. Error bars indicate three experimental replicates. Source data are available for this figure: .

    Article Snippet: Biotin-X ANXA5 (5 μl) (Thermo Fisher Scientific) was added, mixed, and incubated for 15 min at room temperature.

    Techniques: Clinical Proteomics, Membrane, Expressing, Staining, Concentration Assay, Western Blot, Mutagenesis, Control