biotin sp conjugated affinipure goat anti mouse igg  (Jackson Immuno)

 
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    Name:
    Biotin SP AffiniPure Goat Anti Mouse IgG Fcγ Subclass 1 Specific
    Description:
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on antigen binding assay and or ELISA the antibody reacts with the Fc portion of mouse IgG1 but not with other mouse IgG subclasses mouse IgM or the Fab portion of mouse immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with human bovine and rabbit serum proteins but it may cross react with immunoglobulins from other species
    Catalog Number:
    115-065-205
    Price:
    207
    Purity:
    The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
    Conjugate:
    Biotin SP long spacer
    Size:
    ml
    Category:
    Secondary Antibody
    Source:
    Goat
    Quantity:
    0 5
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    Structured Review

    Jackson Immuno biotin sp conjugated affinipure goat anti mouse igg
    Biotin SP AffiniPure Goat Anti Mouse IgG Fcγ Subclass 1 Specific
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on antigen binding assay and or ELISA the antibody reacts with the Fc portion of mouse IgG1 but not with other mouse IgG subclasses mouse IgM or the Fab portion of mouse immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with human bovine and rabbit serum proteins but it may cross react with immunoglobulins from other species
    https://www.bioz.com/result/biotin sp conjugated affinipure goat anti mouse igg/product/Jackson Immuno
    Average 90 stars, based on 497 article reviews
    Price from $9.99 to $1999.99
    biotin sp conjugated affinipure goat anti mouse igg - by Bioz Stars, 2020-04
    90/100 stars

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    Related Articles

    TUNEL Assay:

    Article Title: Estrogen Regulates the Satellite Cell Compartment in Females
    Article Snippet: Following TUNEL, Pax7 staining was completed as previously reported ( ; ). .. Slides were incubated with goat anti-mouse biotin-conjugated secondary antibody (115-065-205, Jackson Immuno Research Laboratories Inc, West Grove, PA, 1:1000 in 3% BSA) for 1 h at room temperature.

    Immunohistochemistry:

    Article Title: Fiber Type-Specific Satellite Cell Content in Cyclists Following Heavy Training with Carbohydrate and Carbohydrate-Protein Supplementation
    Article Snippet: Paragraph title: Immunohistochemistry: Pax7, laminin, MyHC I ... Day 3: Slides were then incubated for 1 h at room temperature in goat anti-mouse biotinylated secondary antibody (Cat# 115-065-205, 1:1000 in 2.5% NHS, Jackson Immuno Research, West Grove, PA).

    Article Title: Blood Flow Deficits and Cerebrovascular Changes in a Dietary Model of Hyperhomocysteinemia
    Article Snippet: .. BBB Permeability Measurement by Immunoglobulin G Immunohistochemistry For immunoglobulin G (IgG) detection, samples underwent endogenous peroxidase quench for 30 min (0.3% hydrogen peroxide in methanol) followed by incubation for 1 hr at 4°C with a 1:250 concentration of biotinylated ant-imouse IgG antibody (Jackson ImmunoResearch, West Grove, PA, USA, #115-065-205) in PBS with 0.2% TX-100. .. Subsequently, tissue was incubated for 1 hr in ABC solution (Vector Laboratories, Burlingame, CA, USA, #PK-4000) and developed with 3,3′-diaminobenzidine tetrahydrochloride hydrate (Millipore Sigma #D5637).

    Article Title: Satellite cell activation and apoptosis in skeletal muscle from severely burned children
    Article Snippet: Paragraph title: Immunohistochemistry ... The following day, slides were incubated in goat anti‐rabbit AF488 and goat anti‐mouse biotin secondary antibody (no. 115‐065‐205; Jackson Immuno Research, West Grove, PA, USA) for 1 h, and reacted with streptavidin–horseradish peroxidase and AF555 tyramide included with the tyramide signal amplification (TSA) kit (no. , Life Technologies).

    Article Title: Life-long reduction in myomiR expression does not adversely affect skeletal muscle morphology
    Article Snippet: Paragraph title: Immunohistochemistry ... After washing, incubation with anti-dystrophin antibody (1:50, vector # VP D505) overnight (4 °C) was followed by incubation for 75 min with goat anti-mouse biotinylated secondary antibody (1:1000 in 1% TSA blocking, Jackson Immuno Research # 115-065-205).

    Article Title: Parity predisposes breasts to the oncogenic action of PAPP-A and activation of the collagen receptor DDR2
    Article Snippet: Paragraph title: Histology and immunohistochemistry ... Biotinylated mouse antibody (Jackson ImmunoResearch #115-065-205) diluted 1:200 in TBS for 1 h at room temperature was used for the secondary antibody incubation.

    Article Title: Chronic doxorubicin administration impacts satellite cell and capillary abundance in a muscle‐specific manner. Chronic doxorubicin administration impacts satellite cell and capillary abundance in a muscle‐specific manner
    Article Snippet: Paragraph title: Immunohistochemistry ... The following morning, slides were incubated in AF647 conjugated‐wheat germ agglutinin (WGA, #W32466; Invitrogen), goat anti‐rabbit AF555 (#A21424; Invitrogen) and goat anti‐mouse biotin secondary antibody (no. 115‐065‐205; Jackson ImmunoResearch) for 1 h, and reacted with streptavidin–horseradish peroxidase and AF488 tyramide included with the TSA kit (#T20932, Invitrogen).

    Amplification:

    Article Title: Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise. Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise
    Article Snippet: On the second day of staining a 3 × 3 min wash in PBS proceeded a 80 min incubation in goat anti‐rabbit IgG AF555 (1:250) (Invitrogen, Cat # A‐21429) and goat anti‐mouse IgG biotin –SP‐conjugated (1:1000) (Jackson Immuno Research, Cat #115‐065‐205). .. Sections were washed 3 × 3 min in PBS and then exposed to a 1 h incubation of Streptavidin‐horseradish peroxidase conjugate (1:100) in PBS, washed 3 × 3 min in PBS, and incubated for 20 min in Alexa Fluor 488 (1:200, (Invitrogen Cat# T20932)) in amplification diluents.

    Article Title: Fiber Type-Specific Satellite Cell Content in Cyclists Following Heavy Training with Carbohydrate and Carbohydrate-Protein Supplementation
    Article Snippet: Day 3: Slides were then incubated for 1 h at room temperature in goat anti-mouse biotinylated secondary antibody (Cat# 115-065-205, 1:1000 in 2.5% NHS, Jackson Immuno Research, West Grove, PA). .. Utilizing a tyramide signal amplification (TSA) kit (T20932, Life Technologies), slides were incubated for 1 h in streptavidin-horseradish peroxidase (1:100), and then for 20 min in AF488 tyramide (1:200).

    Article Title: Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise. Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise
    Article Snippet: .. On day 3 sections were incubated with goat anti‐mouse IgG biotin –SP‐conjugated (1:1000) (Jackson Immuno Research, Cat #115‐065‐205), streptavidin‐horseradish peroxidase conjugate and then reacted with the tyramide signal amplification kit (ThermoFisher, Cat #T20932) followed by mounting in DAPI containing mounting media (Vector) and allowed to air dry. .. The staining protocol resulted in DAPI‐positive nuclei staining blue, Pax7 + cells (stained yellow), MHC I (stained purple), MHC II (Black‐negative staining) and laminin basement membrane (stained red) of a muscle fiber.

    Article Title: Walking performance is positively correlated to calf muscle fiber size in peripheral artery disease subjects, but fibers show aberrant mitophagy: an observational study
    Article Snippet: .. Following TBST washes, slides were incubated in biotin goat anti-mouse IgG1 (#115-065-205; Jackson Immuno Research, West Grove, PA, USA) and anti-mouse IgG2a AF488 (#A21121; Invitrogen) secondary antibodies in TBST with 1 % normal goat serum for 1 h. Slides were reacted with streptavidin-horseradish peroxidase included with the TSA kit (#T20935; Invitrogen), incubated in TSA AF594 (#T20950; Invitrogen) in amplification diluents for 15 min, then mounted (#H-1000; Vector Laboratories). .. For staining of mitochondrial COX-1 and microtubule-associated protein light chain 3 (LC3), slides were fixed in 4 % paraformaldehyde for 10 min, washed with TBST (1 % Tween-20), then blocked for 1 h in TBST with 5 % normal goat serum.

    Article Title: Estrogen Regulates the Satellite Cell Compartment in Females
    Article Snippet: Slides were incubated with goat anti-mouse biotin-conjugated secondary antibody (115-065-205, Jackson Immuno Research Laboratories Inc, West Grove, PA, 1:1000 in 3% BSA) for 1 h at room temperature. .. Visualization of the primary antibody was performed using the Vectastain ABC kit (PK-6100, Vector Laboratories, Burlingame, CA) and Tyramide Signal Amplification (TSA) Plus Cyanine 3 kit (NEL744, PerkinElmer, Waltham, MA, 1:50 in diluent buffer).

    Article Title: Satellite cell activation and apoptosis in skeletal muscle from severely burned children
    Article Snippet: .. The following day, slides were incubated in goat anti‐rabbit AF488 and goat anti‐mouse biotin secondary antibody (no. 115‐065‐205; Jackson Immuno Research, West Grove, PA, USA) for 1 h, and reacted with streptavidin–horseradish peroxidase and AF555 tyramide included with the tyramide signal amplification (TSA) kit (no. , Life Technologies). ..

    Article Title: Life-long reduction in myomiR expression does not adversely affect skeletal muscle morphology
    Article Snippet: Incubation with anti-Pax7 antibody (1:100, Developmental Studies Hybridoma Bank, Iowa City, IA) was followed by incubation with the biotin-conjugated secondary antibody and streptavidin-HRP included within the Tyramide Signal Amplification kit (#T20935, Invitrogen, Carlsbad, CA). .. After washing, incubation with anti-dystrophin antibody (1:50, vector # VP D505) overnight (4 °C) was followed by incubation for 75 min with goat anti-mouse biotinylated secondary antibody (1:1000 in 1% TSA blocking, Jackson Immuno Research # 115-065-205).

    Whole Genome Amplification:

    Article Title: Life-long reduction in myomiR expression does not adversely affect skeletal muscle morphology
    Article Snippet: After washing, incubation with anti-dystrophin antibody (1:50, vector # VP D505) overnight (4 °C) was followed by incubation for 75 min with goat anti-mouse biotinylated secondary antibody (1:1000 in 1% TSA blocking, Jackson Immuno Research # 115-065-205). .. For N-acetyl-d-glucosamine staining, muscle sections were fixed with 4% PFA and then incubated with Texas red directly conjugated wheat germ agglutinin (WGA).

    Article Title: Chronic doxorubicin administration impacts satellite cell and capillary abundance in a muscle‐specific manner. Chronic doxorubicin administration impacts satellite cell and capillary abundance in a muscle‐specific manner
    Article Snippet: .. The following morning, slides were incubated in AF647 conjugated‐wheat germ agglutinin (WGA, #W32466; Invitrogen), goat anti‐rabbit AF555 (#A21424; Invitrogen) and goat anti‐mouse biotin secondary antibody (no. 115‐065‐205; Jackson ImmunoResearch) for 1 h, and reacted with streptavidin–horseradish peroxidase and AF488 tyramide included with the TSA kit (#T20932, Invitrogen). .. Slides were co‐stained with DAPI prior to being mounted with Vectashield fluorescent mounting media (Vector Laboratories, Burlingame, CA).

    In Situ:

    Article Title: Estrogen Regulates the Satellite Cell Compartment in Females
    Article Snippet: TUNEL was detected using In Situ Cell Death, Fluorescein Detection Kit according to manufacturer’s instructions (11684795910, Roche Diagnostics, Mannheim, Germany). .. Slides were incubated with goat anti-mouse biotin-conjugated secondary antibody (115-065-205, Jackson Immuno Research Laboratories Inc, West Grove, PA, 1:1000 in 3% BSA) for 1 h at room temperature.

    Article Title: Satellite cell activation and apoptosis in skeletal muscle from severely burned children
    Article Snippet: The following day, slides were incubated in goat anti‐rabbit AF488 and goat anti‐mouse biotin secondary antibody (no. 115‐065‐205; Jackson Immuno Research, West Grove, PA, USA) for 1 h, and reacted with streptavidin–horseradish peroxidase and AF555 tyramide included with the tyramide signal amplification (TSA) kit (no. , Life Technologies). .. For Pax7/TUNEL/laminin staining, slides were fixed in 4% PFA for 10 min and then incubated in the In Situ Cell Death Detection Kit per manufacturer's instructions.

    Software:

    Article Title: Blood Flow Deficits and Cerebrovascular Changes in a Dietary Model of Hyperhomocysteinemia
    Article Snippet: BBB Permeability Measurement by Immunoglobulin G Immunohistochemistry For immunoglobulin G (IgG) detection, samples underwent endogenous peroxidase quench for 30 min (0.3% hydrogen peroxide in methanol) followed by incubation for 1 hr at 4°C with a 1:250 concentration of biotinylated ant-imouse IgG antibody (Jackson ImmunoResearch, West Grove, PA, USA, #115-065-205) in PBS with 0.2% TX-100. .. Slides were imaged at 20× using an Aperio ScanScope XT digital slide scanner (Leica Biosystems), and cortex was outlined using the Aperio ImageScope software (Leica Biosystems) as previously reported ( ).

    Article Title: Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise. Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise
    Article Snippet: On day 3 sections were incubated with goat anti‐mouse IgG biotin –SP‐conjugated (1:1000) (Jackson Immuno Research, Cat #115‐065‐205), streptavidin‐horseradish peroxidase conjugate and then reacted with the tyramide signal amplification kit (ThermoFisher, Cat #T20932) followed by mounting in DAPI containing mounting media (Vector) and allowed to air dry. .. Myonuclei were manually counted in images captured with a 100–400× total magnification using AxioVision 4.9.1 software to determine the number of myonuclei per fiber.

    Microscopy:

    Article Title: Estrogen Regulates the Satellite Cell Compartment in Females
    Article Snippet: Paragraph title: Immunofluorescence Microscopy in Mouse Samples ... Slides were incubated with goat anti-mouse biotin-conjugated secondary antibody (115-065-205, Jackson Immuno Research Laboratories Inc, West Grove, PA, 1:1000 in 3% BSA) for 1 h at room temperature.

    Plasmid Preparation:

    Article Title: Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise. Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise
    Article Snippet: On the second day of staining a 3 × 3 min wash in PBS proceeded a 80 min incubation in goat anti‐rabbit IgG AF555 (1:250) (Invitrogen, Cat # A‐21429) and goat anti‐mouse IgG biotin –SP‐conjugated (1:1000) (Jackson Immuno Research, Cat #115‐065‐205). .. After a quick wash slides were mounted in medium mounting media (Vector) and allowed to air dry prior to imaging.

    Article Title: Fiber Type-Specific Satellite Cell Content in Cyclists Following Heavy Training with Carbohydrate and Carbohydrate-Protein Supplementation
    Article Snippet: Next, slides were washed in PBS and subsequently blocked in 2.5% normal horse serum (NHS) (Vector, S-2012) for 1 h at room temperature, followed by an overnight incubation (4°C) with Pax7 primary antibody (Pax7-C, 1:100, Developmental Studies Hybridoma Bank, Iowa City, Iowa). .. Day 3: Slides were then incubated for 1 h at room temperature in goat anti-mouse biotinylated secondary antibody (Cat# 115-065-205, 1:1000 in 2.5% NHS, Jackson Immuno Research, West Grove, PA).

    Article Title: Blood Flow Deficits and Cerebrovascular Changes in a Dietary Model of Hyperhomocysteinemia
    Article Snippet: BBB Permeability Measurement by Immunoglobulin G Immunohistochemistry For immunoglobulin G (IgG) detection, samples underwent endogenous peroxidase quench for 30 min (0.3% hydrogen peroxide in methanol) followed by incubation for 1 hr at 4°C with a 1:250 concentration of biotinylated ant-imouse IgG antibody (Jackson ImmunoResearch, West Grove, PA, USA, #115-065-205) in PBS with 0.2% TX-100. .. Subsequently, tissue was incubated for 1 hr in ABC solution (Vector Laboratories, Burlingame, CA, USA, #PK-4000) and developed with 3,3′-diaminobenzidine tetrahydrochloride hydrate (Millipore Sigma #D5637).

    Article Title: Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise. Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise
    Article Snippet: .. On day 3 sections were incubated with goat anti‐mouse IgG biotin –SP‐conjugated (1:1000) (Jackson Immuno Research, Cat #115‐065‐205), streptavidin‐horseradish peroxidase conjugate and then reacted with the tyramide signal amplification kit (ThermoFisher, Cat #T20932) followed by mounting in DAPI containing mounting media (Vector) and allowed to air dry. .. The staining protocol resulted in DAPI‐positive nuclei staining blue, Pax7 + cells (stained yellow), MHC I (stained purple), MHC II (Black‐negative staining) and laminin basement membrane (stained red) of a muscle fiber.

    Article Title: Aging-Related effects of Bed Rest followed by Eccentric Exercise Rehabilitation on Skeletal Muscle Macrophages and Insulin Sensitivity
    Article Snippet: Ms IgG blocking buffer (Vector #MKB-2231; 1 drop/ml PBS) at RT and then blocked again in 2.5% NHS for 1 h at RT. .. On day 3 of staining, sections were rinsed with PBS for 3×3 min before and after 1 h incubation with goat anti-mouse IgG biotin-SP-conjugated (Jackson Immuno Research #115-065-205, 1:1000) in 2.5% NHS at RT.

    Article Title: Estrogen Regulates the Satellite Cell Compartment in Females
    Article Snippet: Slides were incubated with goat anti-mouse biotin-conjugated secondary antibody (115-065-205, Jackson Immuno Research Laboratories Inc, West Grove, PA, 1:1000 in 3% BSA) for 1 h at room temperature. .. Visualization of the primary antibody was performed using the Vectastain ABC kit (PK-6100, Vector Laboratories, Burlingame, CA) and Tyramide Signal Amplification (TSA) Plus Cyanine 3 kit (NEL744, PerkinElmer, Waltham, MA, 1:50 in diluent buffer).

    Article Title: Satellite cell activation and apoptosis in skeletal muscle from severely burned children
    Article Snippet: For cleaved caspase‐3/dystrophin staining, slides were fixed in acetone for 10 min, blocked with 5% normal goat serum (no. S‐1000, Vector) and then incubated overnight in anti‐cleaved caspase‐3 (no. 9579, Cell Signaling, Danvers, MA, USA) and anti‐dystrophin. .. The following day, slides were incubated in goat anti‐rabbit AF488 and goat anti‐mouse biotin secondary antibody (no. 115‐065‐205; Jackson Immuno Research, West Grove, PA, USA) for 1 h, and reacted with streptavidin–horseradish peroxidase and AF555 tyramide included with the tyramide signal amplification (TSA) kit (no. , Life Technologies).

    Article Title: Life-long reduction in myomiR expression does not adversely affect skeletal muscle morphology
    Article Snippet: .. After washing, incubation with anti-dystrophin antibody (1:50, vector # VP D505) overnight (4 °C) was followed by incubation for 75 min with goat anti-mouse biotinylated secondary antibody (1:1000 in 1% TSA blocking, Jackson Immuno Research # 115-065-205). .. Sections were washed again, incubated 30 min in SA-FITC (1:150, Vector # SA-5001), and post-fixed in 4% paraformaldehyde before mounting using Vectashield fluorescent mounting media with DAPI.

    Article Title: Aging-Related effects of Bed Rest followed by Eccentric Exercise Rehabilitation on Skeletal Muscle Macrophages and Insulin Sensitivity
    Article Snippet: Following three 3 min washes in PBS, sections were blocked for 1 h in 2.5% normal horse serum (NHS, Vector, S-2012) at RT and subsequently incubated overnight at 4C with primary antibodies diluted in 2.5% NHS against rabbit dystrophin (Santa Cruz #sc-15376, 1:100) and mouse CD68 (Dako #M0814, 1:100). .. On day 2 sections were incubated for 1 h with secondary antibodies: Alexa Fluor 647 conjugated goat anti-rabbit IgG (Invitrogen #21245, 1:500, for dystrophin) and biotin-SP-conjugated goat anti-mouse IgG (Jackson Immuno Research #115-065-205, 1:1000, for CD68) diluted in 2.5% NHS at RT in the dark.

    Article Title: Parity predisposes breasts to the oncogenic action of PAPP-A and activation of the collagen receptor DDR2
    Article Snippet: Biotinylated mouse antibody (Jackson ImmunoResearch #115-065-205) diluted 1:200 in TBS for 1 h at room temperature was used for the secondary antibody incubation. .. Biotinylated mouse antibody (Jackson ImmunoResearch #115-065-205) diluted 1:200 in TBS for 1 h at room temperature was used for the secondary antibody incubation.

    Article Title: Chronic doxorubicin administration impacts satellite cell and capillary abundance in a muscle‐specific manner. Chronic doxorubicin administration impacts satellite cell and capillary abundance in a muscle‐specific manner
    Article Snippet: The following morning, slides were incubated in AF647 conjugated‐wheat germ agglutinin (WGA, #W32466; Invitrogen), goat anti‐rabbit AF555 (#A21424; Invitrogen) and goat anti‐mouse biotin secondary antibody (no. 115‐065‐205; Jackson ImmunoResearch) for 1 h, and reacted with streptavidin–horseradish peroxidase and AF488 tyramide included with the TSA kit (#T20932, Invitrogen). .. The following morning, slides were incubated in AF647 conjugated‐wheat germ agglutinin (WGA, #W32466; Invitrogen), goat anti‐rabbit AF555 (#A21424; Invitrogen) and goat anti‐mouse biotin secondary antibody (no. 115‐065‐205; Jackson ImmunoResearch) for 1 h, and reacted with streptavidin–horseradish peroxidase and AF488 tyramide included with the TSA kit (#T20932, Invitrogen).

    Concentration Assay:

    Article Title: Blood Flow Deficits and Cerebrovascular Changes in a Dietary Model of Hyperhomocysteinemia
    Article Snippet: .. BBB Permeability Measurement by Immunoglobulin G Immunohistochemistry For immunoglobulin G (IgG) detection, samples underwent endogenous peroxidase quench for 30 min (0.3% hydrogen peroxide in methanol) followed by incubation for 1 hr at 4°C with a 1:250 concentration of biotinylated ant-imouse IgG antibody (Jackson ImmunoResearch, West Grove, PA, USA, #115-065-205) in PBS with 0.2% TX-100. .. Subsequently, tissue was incubated for 1 hr in ABC solution (Vector Laboratories, Burlingame, CA, USA, #PK-4000) and developed with 3,3′-diaminobenzidine tetrahydrochloride hydrate (Millipore Sigma #D5637).

    Incubation:

    Article Title: Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise. Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise
    Article Snippet: .. On the second day of staining a 3 × 3 min wash in PBS proceeded a 80 min incubation in goat anti‐rabbit IgG AF555 (1:250) (Invitrogen, Cat # A‐21429) and goat anti‐mouse IgG biotin –SP‐conjugated (1:1000) (Jackson Immuno Research, Cat #115‐065‐205). .. Sections were washed 3 × 3 min in PBS and then exposed to a 1 h incubation of Streptavidin‐horseradish peroxidase conjugate (1:100) in PBS, washed 3 × 3 min in PBS, and incubated for 20 min in Alexa Fluor 488 (1:200, (Invitrogen Cat# T20932)) in amplification diluents.

    Article Title: Fiber Type-Specific Satellite Cell Content in Cyclists Following Heavy Training with Carbohydrate and Carbohydrate-Protein Supplementation
    Article Snippet: .. Day 3: Slides were then incubated for 1 h at room temperature in goat anti-mouse biotinylated secondary antibody (Cat# 115-065-205, 1:1000 in 2.5% NHS, Jackson Immuno Research, West Grove, PA). .. Utilizing a tyramide signal amplification (TSA) kit (T20932, Life Technologies), slides were incubated for 1 h in streptavidin-horseradish peroxidase (1:100), and then for 20 min in AF488 tyramide (1:200).

    Article Title: Blood Flow Deficits and Cerebrovascular Changes in a Dietary Model of Hyperhomocysteinemia
    Article Snippet: .. BBB Permeability Measurement by Immunoglobulin G Immunohistochemistry For immunoglobulin G (IgG) detection, samples underwent endogenous peroxidase quench for 30 min (0.3% hydrogen peroxide in methanol) followed by incubation for 1 hr at 4°C with a 1:250 concentration of biotinylated ant-imouse IgG antibody (Jackson ImmunoResearch, West Grove, PA, USA, #115-065-205) in PBS with 0.2% TX-100. .. Subsequently, tissue was incubated for 1 hr in ABC solution (Vector Laboratories, Burlingame, CA, USA, #PK-4000) and developed with 3,3′-diaminobenzidine tetrahydrochloride hydrate (Millipore Sigma #D5637).

    Article Title: Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise. Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise
    Article Snippet: .. On day 3 sections were incubated with goat anti‐mouse IgG biotin –SP‐conjugated (1:1000) (Jackson Immuno Research, Cat #115‐065‐205), streptavidin‐horseradish peroxidase conjugate and then reacted with the tyramide signal amplification kit (ThermoFisher, Cat #T20932) followed by mounting in DAPI containing mounting media (Vector) and allowed to air dry. .. The staining protocol resulted in DAPI‐positive nuclei staining blue, Pax7 + cells (stained yellow), MHC I (stained purple), MHC II (Black‐negative staining) and laminin basement membrane (stained red) of a muscle fiber.

    Article Title: Walking performance is positively correlated to calf muscle fiber size in peripheral artery disease subjects, but fibers show aberrant mitophagy: an observational study
    Article Snippet: .. Following TBST washes, slides were incubated in biotin goat anti-mouse IgG1 (#115-065-205; Jackson Immuno Research, West Grove, PA, USA) and anti-mouse IgG2a AF488 (#A21121; Invitrogen) secondary antibodies in TBST with 1 % normal goat serum for 1 h. Slides were reacted with streptavidin-horseradish peroxidase included with the TSA kit (#T20935; Invitrogen), incubated in TSA AF594 (#T20950; Invitrogen) in amplification diluents for 15 min, then mounted (#H-1000; Vector Laboratories). .. For staining of mitochondrial COX-1 and microtubule-associated protein light chain 3 (LC3), slides were fixed in 4 % paraformaldehyde for 10 min, washed with TBST (1 % Tween-20), then blocked for 1 h in TBST with 5 % normal goat serum.

    Article Title: Aging-Related effects of Bed Rest followed by Eccentric Exercise Rehabilitation on Skeletal Muscle Macrophages and Insulin Sensitivity
    Article Snippet: .. On day 3 of staining, sections were rinsed with PBS for 3×3 min before and after 1 h incubation with goat anti-mouse IgG biotin-SP-conjugated (Jackson Immuno Research #115-065-205, 1:1000) in 2.5% NHS at RT. .. Sections were exposed to a 1 h incubation of SA-HRP (1:500 in PBS), washed in 3×3 min intervals in PBS, and incubated for 15 min in Alexa Fluor 568 (Invitrogen # ) in amplification diluents.

    Article Title: Estrogen Regulates the Satellite Cell Compartment in Females
    Article Snippet: .. Slides were incubated with goat anti-mouse biotin-conjugated secondary antibody (115-065-205, Jackson Immuno Research Laboratories Inc, West Grove, PA, 1:1000 in 3% BSA) for 1 h at room temperature. .. Visualization of the primary antibody was performed using the Vectastain ABC kit (PK-6100, Vector Laboratories, Burlingame, CA) and Tyramide Signal Amplification (TSA) Plus Cyanine 3 kit (NEL744, PerkinElmer, Waltham, MA, 1:50 in diluent buffer).

    Article Title: Satellite cell activation and apoptosis in skeletal muscle from severely burned children
    Article Snippet: .. The following day, slides were incubated in goat anti‐rabbit AF488 and goat anti‐mouse biotin secondary antibody (no. 115‐065‐205; Jackson Immuno Research, West Grove, PA, USA) for 1 h, and reacted with streptavidin–horseradish peroxidase and AF555 tyramide included with the tyramide signal amplification (TSA) kit (no. , Life Technologies). ..

    Article Title: Life-long reduction in myomiR expression does not adversely affect skeletal muscle morphology
    Article Snippet: .. After washing, incubation with anti-dystrophin antibody (1:50, vector # VP D505) overnight (4 °C) was followed by incubation for 75 min with goat anti-mouse biotinylated secondary antibody (1:1000 in 1% TSA blocking, Jackson Immuno Research # 115-065-205). .. Sections were washed again, incubated 30 min in SA-FITC (1:150, Vector # SA-5001), and post-fixed in 4% paraformaldehyde before mounting using Vectashield fluorescent mounting media with DAPI.

    Article Title: Aging-Related effects of Bed Rest followed by Eccentric Exercise Rehabilitation on Skeletal Muscle Macrophages and Insulin Sensitivity
    Article Snippet: .. On day 2 sections were incubated for 1 h with secondary antibodies: Alexa Fluor 647 conjugated goat anti-rabbit IgG (Invitrogen #21245, 1:500, for dystrophin) and biotin-SP-conjugated goat anti-mouse IgG (Jackson Immuno Research #115-065-205, 1:1000, for CD68) diluted in 2.5% NHS at RT in the dark. .. After 3×3 min rinses with PBS, sections were incubated for 1 h with streptavidin-horseradish peroxidase conjugate (SA-HRP, Invitrogen, S-911, 1:500 in PBS) and rinsed again with PBS were repeated following this step.

    Article Title: Parity predisposes breasts to the oncogenic action of PAPP-A and activation of the collagen receptor DDR2
    Article Snippet: .. Biotinylated mouse antibody (Jackson ImmunoResearch #115-065-205) diluted 1:200 in TBS for 1 h at room temperature was used for the secondary antibody incubation. ..

    Article Title: Chronic doxorubicin administration impacts satellite cell and capillary abundance in a muscle‐specific manner. Chronic doxorubicin administration impacts satellite cell and capillary abundance in a muscle‐specific manner
    Article Snippet: .. The following morning, slides were incubated in AF647 conjugated‐wheat germ agglutinin (WGA, #W32466; Invitrogen), goat anti‐rabbit AF555 (#A21424; Invitrogen) and goat anti‐mouse biotin secondary antibody (no. 115‐065‐205; Jackson ImmunoResearch) for 1 h, and reacted with streptavidin–horseradish peroxidase and AF488 tyramide included with the TSA kit (#T20932, Invitrogen). .. Slides were co‐stained with DAPI prior to being mounted with Vectashield fluorescent mounting media (Vector Laboratories, Burlingame, CA).

    Activity Assay:

    Article Title: Fiber Type-Specific Satellite Cell Content in Cyclists Following Heavy Training with Carbohydrate and Carbohydrate-Protein Supplementation
    Article Snippet: Day 2: Following a wash in PBS, endogenous peroxidase activity was blocked with a 7-min wash in 3% H2 O2 . .. Day 3: Slides were then incubated for 1 h at room temperature in goat anti-mouse biotinylated secondary antibody (Cat# 115-065-205, 1:1000 in 2.5% NHS, Jackson Immuno Research, West Grove, PA).

    Article Title: Life-long reduction in myomiR expression does not adversely affect skeletal muscle morphology
    Article Snippet: Following the overnight incubation, muscle sections were subject to epitope retrieval using sodium citrate (10 mM, pH 6.5) at 92 °C for 11 min. Endogenous peroxidase activity was blocked with 3% hydrogen peroxide in PBS followed by an additional blocking step with Mouse-on-Mouse Blocking Reagent (Vector Laboratories, Burlingame, CA). .. After washing, incubation with anti-dystrophin antibody (1:50, vector # VP D505) overnight (4 °C) was followed by incubation for 75 min with goat anti-mouse biotinylated secondary antibody (1:1000 in 1% TSA blocking, Jackson Immuno Research # 115-065-205).

    Immunostaining:

    Article Title: Parity predisposes breasts to the oncogenic action of PAPP-A and activation of the collagen receptor DDR2
    Article Snippet: For immunostaining of paraffin-embedded sections, the samples were deparaffinized in xylene and rehydrated in water. .. Biotinylated mouse antibody (Jackson ImmunoResearch #115-065-205) diluted 1:200 in TBS for 1 h at room temperature was used for the secondary antibody incubation.

    Mass Spectrometry:

    Article Title: Aging-Related effects of Bed Rest followed by Eccentric Exercise Rehabilitation on Skeletal Muscle Macrophages and Insulin Sensitivity
    Article Snippet: Ms IgG blocking buffer (Vector #MKB-2231; 1 drop/ml PBS) at RT and then blocked again in 2.5% NHS for 1 h at RT. .. On day 3 of staining, sections were rinsed with PBS for 3×3 min before and after 1 h incubation with goat anti-mouse IgG biotin-SP-conjugated (Jackson Immuno Research #115-065-205, 1:1000) in 2.5% NHS at RT.

    Permeability:

    Article Title: Blood Flow Deficits and Cerebrovascular Changes in a Dietary Model of Hyperhomocysteinemia
    Article Snippet: .. BBB Permeability Measurement by Immunoglobulin G Immunohistochemistry For immunoglobulin G (IgG) detection, samples underwent endogenous peroxidase quench for 30 min (0.3% hydrogen peroxide in methanol) followed by incubation for 1 hr at 4°C with a 1:250 concentration of biotinylated ant-imouse IgG antibody (Jackson ImmunoResearch, West Grove, PA, USA, #115-065-205) in PBS with 0.2% TX-100. .. Subsequently, tissue was incubated for 1 hr in ABC solution (Vector Laboratories, Burlingame, CA, USA, #PK-4000) and developed with 3,3′-diaminobenzidine tetrahydrochloride hydrate (Millipore Sigma #D5637).

    IA:

    Article Title: Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise. Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise
    Article Snippet: On day 2 sections were incubated in Alexa Fluor 647‐conjugated goat anti‐mouse IgG2b (for MHC I: 1:250, Invitrogen, Carlsbad, CA) and Alexa Fluor 594 goat anti‐rat IgG1 (for laminin: 1:500, Invitrogen, Carlsbad, CA), washed and then incubated with a primary antibody against Pax7 (1:100, Developmental Studies Hybridoma Bank, Iowa City, IA). .. On day 3 sections were incubated with goat anti‐mouse IgG biotin –SP‐conjugated (1:1000) (Jackson Immuno Research, Cat #115‐065‐205), streptavidin‐horseradish peroxidase conjugate and then reacted with the tyramide signal amplification kit (ThermoFisher, Cat #T20932) followed by mounting in DAPI containing mounting media (Vector) and allowed to air dry.

    Article Title: Life-long reduction in myomiR expression does not adversely affect skeletal muscle morphology
    Article Snippet: Incubation with anti-Pax7 antibody (1:100, Developmental Studies Hybridoma Bank, Iowa City, IA) was followed by incubation with the biotin-conjugated secondary antibody and streptavidin-HRP included within the Tyramide Signal Amplification kit (#T20935, Invitrogen, Carlsbad, CA). .. After washing, incubation with anti-dystrophin antibody (1:50, vector # VP D505) overnight (4 °C) was followed by incubation for 75 min with goat anti-mouse biotinylated secondary antibody (1:1000 in 1% TSA blocking, Jackson Immuno Research # 115-065-205).

    Staining:

    Article Title: Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise. Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise
    Article Snippet: .. On the second day of staining a 3 × 3 min wash in PBS proceeded a 80 min incubation in goat anti‐rabbit IgG AF555 (1:250) (Invitrogen, Cat # A‐21429) and goat anti‐mouse IgG biotin –SP‐conjugated (1:1000) (Jackson Immuno Research, Cat #115‐065‐205). .. Sections were washed 3 × 3 min in PBS and then exposed to a 1 h incubation of Streptavidin‐horseradish peroxidase conjugate (1:100) in PBS, washed 3 × 3 min in PBS, and incubated for 20 min in Alexa Fluor 488 (1:200, (Invitrogen Cat# T20932)) in amplification diluents.

    Article Title: Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise. Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise
    Article Snippet: On day 3 sections were incubated with goat anti‐mouse IgG biotin –SP‐conjugated (1:1000) (Jackson Immuno Research, Cat #115‐065‐205), streptavidin‐horseradish peroxidase conjugate and then reacted with the tyramide signal amplification kit (ThermoFisher, Cat #T20932) followed by mounting in DAPI containing mounting media (Vector) and allowed to air dry. .. The staining protocol resulted in DAPI‐positive nuclei staining blue, Pax7 + cells (stained yellow), MHC I (stained purple), MHC II (Black‐negative staining) and laminin basement membrane (stained red) of a muscle fiber.

    Article Title: Walking performance is positively correlated to calf muscle fiber size in peripheral artery disease subjects, but fibers show aberrant mitophagy: an observational study
    Article Snippet: For staining of mitochondrial complex I, subunit 20 and complex IV, subunit 1 (COX-1), slides were fixed in 4 % paraformaldehyde for 10 min, washed with TBST (1 % Tween-20), then blocked for 1 h in TBST with 1 % normal goat serum. .. Following TBST washes, slides were incubated in biotin goat anti-mouse IgG1 (#115-065-205; Jackson Immuno Research, West Grove, PA, USA) and anti-mouse IgG2a AF488 (#A21121; Invitrogen) secondary antibodies in TBST with 1 % normal goat serum for 1 h. Slides were reacted with streptavidin-horseradish peroxidase included with the TSA kit (#T20935; Invitrogen), incubated in TSA AF594 (#T20950; Invitrogen) in amplification diluents for 15 min, then mounted (#H-1000; Vector Laboratories).

    Article Title: Aging-Related effects of Bed Rest followed by Eccentric Exercise Rehabilitation on Skeletal Muscle Macrophages and Insulin Sensitivity
    Article Snippet: .. On day 3 of staining, sections were rinsed with PBS for 3×3 min before and after 1 h incubation with goat anti-mouse IgG biotin-SP-conjugated (Jackson Immuno Research #115-065-205, 1:1000) in 2.5% NHS at RT. .. Sections were exposed to a 1 h incubation of SA-HRP (1:500 in PBS), washed in 3×3 min intervals in PBS, and incubated for 15 min in Alexa Fluor 568 (Invitrogen # ) in amplification diluents.

    Article Title: Estrogen Regulates the Satellite Cell Compartment in Females
    Article Snippet: Following TUNEL, Pax7 staining was completed as previously reported ( ; ). .. Slides were incubated with goat anti-mouse biotin-conjugated secondary antibody (115-065-205, Jackson Immuno Research Laboratories Inc, West Grove, PA, 1:1000 in 3% BSA) for 1 h at room temperature.

    Article Title: Satellite cell activation and apoptosis in skeletal muscle from severely burned children
    Article Snippet: For Pax7/laminin staining, slides were fixed for 10 min in ice‐cold acetone, endogenous peroxidases were blocked with 3% H2 O2 , and then slides were blocked for 1 h in 2.5% normal horse serum. .. The following day, slides were incubated in goat anti‐rabbit AF488 and goat anti‐mouse biotin secondary antibody (no. 115‐065‐205; Jackson Immuno Research, West Grove, PA, USA) for 1 h, and reacted with streptavidin–horseradish peroxidase and AF555 tyramide included with the tyramide signal amplification (TSA) kit (no. , Life Technologies).

    Article Title: Life-long reduction in myomiR expression does not adversely affect skeletal muscle morphology
    Article Snippet: For dystrophin staining, plantaris muscle sections were rehydrated with PBS and blocked in Mouse-on-Mouse Blocking Reagent. .. After washing, incubation with anti-dystrophin antibody (1:50, vector # VP D505) overnight (4 °C) was followed by incubation for 75 min with goat anti-mouse biotinylated secondary antibody (1:1000 in 1% TSA blocking, Jackson Immuno Research # 115-065-205).

    Article Title: Parity predisposes breasts to the oncogenic action of PAPP-A and activation of the collagen receptor DDR2
    Article Snippet: Histology and immunohistochemistry Histological slides were prepared as 4 μm formalin-fixed sections embedded in paraffin and stained with H & E by the Oncological Sciences Department Histology core facility at Icahn School of Medicine at Mount Sinai. .. Biotinylated mouse antibody (Jackson ImmunoResearch #115-065-205) diluted 1:200 in TBS for 1 h at room temperature was used for the secondary antibody incubation.

    Article Title: Chronic doxorubicin administration impacts satellite cell and capillary abundance in a muscle‐specific manner. Chronic doxorubicin administration impacts satellite cell and capillary abundance in a muscle‐specific manner
    Article Snippet: To examine whether DOX administration impacted satellite cell proliferation, we performed Pax7/Ki67 staining. .. The following morning, slides were incubated in AF647 conjugated‐wheat germ agglutinin (WGA, #W32466; Invitrogen), goat anti‐rabbit AF555 (#A21424; Invitrogen) and goat anti‐mouse biotin secondary antibody (no. 115‐065‐205; Jackson ImmunoResearch) for 1 h, and reacted with streptavidin–horseradish peroxidase and AF488 tyramide included with the TSA kit (#T20932, Invitrogen).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise. Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise
    Article Snippet: Sections were incubated for 1 h at RT and then overnight at 4°C with a primary antibody against rabbit anti‐Ki67 (1:100) (Biocare Medical, catCRM325B) and mouse anti‐Pax7 (Concentrate 1:100) from DHSB in 1% TSA at 4°C. .. On the second day of staining a 3 × 3 min wash in PBS proceeded a 80 min incubation in goat anti‐rabbit IgG AF555 (1:250) (Invitrogen, Cat # A‐21429) and goat anti‐mouse IgG biotin –SP‐conjugated (1:1000) (Jackson Immuno Research, Cat #115‐065‐205).

    Binding Assay:

    Article Title: Chronic doxorubicin administration impacts satellite cell and capillary abundance in a muscle‐specific manner. Chronic doxorubicin administration impacts satellite cell and capillary abundance in a muscle‐specific manner
    Article Snippet: TSA‐AF555 was used to visualize Pax7 antibody binding. .. The following morning, slides were incubated in AF647 conjugated‐wheat germ agglutinin (WGA, #W32466; Invitrogen), goat anti‐rabbit AF555 (#A21424; Invitrogen) and goat anti‐mouse biotin secondary antibody (no. 115‐065‐205; Jackson ImmunoResearch) for 1 h, and reacted with streptavidin–horseradish peroxidase and AF488 tyramide included with the TSA kit (#T20932, Invitrogen).

    Immunofluorescence:

    Article Title: Estrogen Regulates the Satellite Cell Compartment in Females
    Article Snippet: Paragraph title: Immunofluorescence Microscopy in Mouse Samples ... Slides were incubated with goat anti-mouse biotin-conjugated secondary antibody (115-065-205, Jackson Immuno Research Laboratories Inc, West Grove, PA, 1:1000 in 3% BSA) for 1 h at room temperature.

    Blocking Assay:

    Article Title: Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise. Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise
    Article Snippet: After a 3 × 3 min rinse in PBS, sections were blocked for 1 h in 1% TSA blocking solution (Invitrogen) at RT. .. On the second day of staining a 3 × 3 min wash in PBS proceeded a 80 min incubation in goat anti‐rabbit IgG AF555 (1:250) (Invitrogen, Cat # A‐21429) and goat anti‐mouse IgG biotin –SP‐conjugated (1:1000) (Jackson Immuno Research, Cat #115‐065‐205).

    Article Title: Aging-Related effects of Bed Rest followed by Eccentric Exercise Rehabilitation on Skeletal Muscle Macrophages and Insulin Sensitivity
    Article Snippet: Ms IgG blocking buffer (Vector #MKB-2231; 1 drop/ml PBS) at RT and then blocked again in 2.5% NHS for 1 h at RT. .. On day 3 of staining, sections were rinsed with PBS for 3×3 min before and after 1 h incubation with goat anti-mouse IgG biotin-SP-conjugated (Jackson Immuno Research #115-065-205, 1:1000) in 2.5% NHS at RT.

    Article Title: Life-long reduction in myomiR expression does not adversely affect skeletal muscle morphology
    Article Snippet: .. After washing, incubation with anti-dystrophin antibody (1:50, vector # VP D505) overnight (4 °C) was followed by incubation for 75 min with goat anti-mouse biotinylated secondary antibody (1:1000 in 1% TSA blocking, Jackson Immuno Research # 115-065-205). .. Sections were washed again, incubated 30 min in SA-FITC (1:150, Vector # SA-5001), and post-fixed in 4% paraformaldehyde before mounting using Vectashield fluorescent mounting media with DAPI.

    Article Title: Aging-Related effects of Bed Rest followed by Eccentric Exercise Rehabilitation on Skeletal Muscle Macrophages and Insulin Sensitivity
    Article Snippet: Sections were incubated for 7 min 3% H₂O₂ in PBS treatment to block endogenous peroxidases. .. On day 2 sections were incubated for 1 h with secondary antibodies: Alexa Fluor 647 conjugated goat anti-rabbit IgG (Invitrogen #21245, 1:500, for dystrophin) and biotin-SP-conjugated goat anti-mouse IgG (Jackson Immuno Research #115-065-205, 1:1000, for CD68) diluted in 2.5% NHS at RT in the dark.

    Article Title: Parity predisposes breasts to the oncogenic action of PAPP-A and activation of the collagen receptor DDR2
    Article Snippet: Peroxidase blocking was completed using endogenous peroxidase solution (Dako Dual Endogenous Enzyme Block #S20003) at room temperature for 15 min. Antibody Diluent (MP Biomedicals Normal Antibody Diluent #980631) was used for serum blocking for 30 min at room temperature. .. Biotinylated mouse antibody (Jackson ImmunoResearch #115-065-205) diluted 1:200 in TBS for 1 h at room temperature was used for the secondary antibody incubation.

    Imaging:

    Article Title: Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise. Postexercise essential amino acid supplementation amplifies skeletal muscle satellite cell proliferation in older men 24 hours postexercise
    Article Snippet: On the second day of staining a 3 × 3 min wash in PBS proceeded a 80 min incubation in goat anti‐rabbit IgG AF555 (1:250) (Invitrogen, Cat # A‐21429) and goat anti‐mouse IgG biotin –SP‐conjugated (1:1000) (Jackson Immuno Research, Cat #115‐065‐205). .. After a quick wash slides were mounted in medium mounting media (Vector) and allowed to air dry prior to imaging.

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    Jackson Immuno biotin sp conjugated affinipure goat anti mouse
    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated <t>AffiniPure</t> goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p
    Biotin Sp Conjugated Affinipure Goat Anti Mouse, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p

    Journal: PLoS ONE

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis

    doi: 10.1371/journal.pone.0109352

    Figure Lengend Snippet: Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p

    Article Snippet: After washing, the cells were stained with biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072), followed by PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) and CD56-FITC.

    Techniques: Flow Cytometry, Cytometry, Cell Culture, Staining, Irradiation

    Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.

    Journal: PLoS ONE

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis

    doi: 10.1371/journal.pone.0109352

    Figure Lengend Snippet: Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.

    Article Snippet: After washing, the cells were stained with biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072), followed by PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) and CD56-FITC.

    Techniques: Immunofluorescence, Staining, Cell Culture