biotin sp affinipure rabbit anti human igm fc5µ fragment specific  (Jackson Immuno)

 
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    Name:
    Biotin SP AffiniPure Rabbit Anti Human IgM Fc5µ Fragment Specific
    Description:
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with the Fc5µ portion of the human IgM heavy chain but not with human IgG IgA or the light chains of human immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with mouse serum proteins but it may cross react with IgM from other species
    Catalog Number:
    309-065-095
    Price:
    198
    Purity:
    The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
    Conjugate:
    Biotin SP long spacer
    Size:
    ml
    Category:
    Secondary Antibody
    Source:
    Rabbit
    Quantity:
    1 0
    Buy from Supplier


    Structured Review

    Jackson Immuno biotin sp affinipure rabbit anti human igm fc5µ fragment specific
    Biotin SP AffiniPure Rabbit Anti Human IgM Fc5µ Fragment Specific
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with the Fc5µ portion of the human IgM heavy chain but not with human IgG IgA or the light chains of human immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with mouse serum proteins but it may cross react with IgM from other species
    https://www.bioz.com/result/biotin sp affinipure rabbit anti human igm fc5µ fragment specific/product/Jackson Immuno
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure rabbit anti human igm fc5µ fragment specific - by Bioz Stars, 2020-07
    85/100 stars

    Images

    1) Product Images from "The Syk-binding Ubiquitin Ligase c-Cbl Mediates Signaling-dependent B Cell Receptor Ubiquitination and B Cell Receptor-mediated Antigen Processing and Presentation *"

    Article Title: The Syk-binding Ubiquitin Ligase c-Cbl Mediates Signaling-dependent B Cell Receptor Ubiquitination and B Cell Receptor-mediated Antigen Processing and Presentation *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M112.357640

    c-Cbl mediates Ag·BCR ubiquitination and processing. Upper panel , B cells were pulsed with anti-human IgM-btn for the indicated time (minutes) at 37 °C. The cells were lysed and ubiquitinated ligand-BCR complexes isolated by ubiquitin
    Figure Legend Snippet: c-Cbl mediates Ag·BCR ubiquitination and processing. Upper panel , B cells were pulsed with anti-human IgM-btn for the indicated time (minutes) at 37 °C. The cells were lysed and ubiquitinated ligand-BCR complexes isolated by ubiquitin

    Techniques Used: Isolation

    2) Product Images from "Cis and Trans Regulatory Mechanisms Control AP2-Mediated B Cell Receptor Endocytosis via Select Tyrosine-Based Motifs"

    Article Title: Cis and Trans Regulatory Mechanisms Control AP2-Mediated B Cell Receptor Endocytosis via Select Tyrosine-Based Motifs

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0054938

    Ultrastructural Colocalization of Ligand-BCR Complexes and AP2 in Clathrin-Coated Pits. A20µWT B cells or primary murine splenocytes were pulsed with anti-IgM-btn followed by anti-biotin-15 nm gold (arrow heads), incubated 2 minutes at 37° and then plasma membrane rips were prepared as previously reported [10] . The exposed cytoplasmic face of the plasma membrane was stained with anti-AP2 and Protein A-5 nm gold (arrows). The percent of BCR-containing CCP that also stained for AP2 is indicated below each image. Inset: AP2 and BCR co-localization within electron dense membrane regions lacking discernable CCP architecture. Shown are representative images from 1 of 3 experiments, with 1,000+ BCR-bound gold particles or 100+ CCP photographed cumulatively.
    Figure Legend Snippet: Ultrastructural Colocalization of Ligand-BCR Complexes and AP2 in Clathrin-Coated Pits. A20µWT B cells or primary murine splenocytes were pulsed with anti-IgM-btn followed by anti-biotin-15 nm gold (arrow heads), incubated 2 minutes at 37° and then plasma membrane rips were prepared as previously reported [10] . The exposed cytoplasmic face of the plasma membrane was stained with anti-AP2 and Protein A-5 nm gold (arrows). The percent of BCR-containing CCP that also stained for AP2 is indicated below each image. Inset: AP2 and BCR co-localization within electron dense membrane regions lacking discernable CCP architecture. Shown are representative images from 1 of 3 experiments, with 1,000+ BCR-bound gold particles or 100+ CCP photographed cumulatively.

    Techniques Used: Incubation, Staining

    Related Articles

    CtB Assay:

    Article Title: Physiological-Range Temperature Changes Modulate Cognate Antigen Processing and Presentation Mediated by Lipid Raft-Restricted Ubiquitinated B Cell Receptor Molecules
    Article Snippet: .. The following reagents were used for this study: hen egg lysozyme (HEL, catalog no. L-6876; Sigma, St Louis, MO), Aw3.18 mAb recognizing peptide residues 48–62 of HEL bound to MHC II I-Ak (murine IgG1, Catalog no. CRL-2826 ATCC, Manassas, VA), Goat anti-mouse IgG F(ab′)2 (Catalog no. .115-006-006; Jackson ImmunoResearch, West Grove, PA), FITC Rat anti-mouse IgG1 (A85-1, Catalog no. 553443; BD Pharmingen, San Jose, CA), FITC anti-mouse I-Ak (10-3.6, IgG2a; Catalog no. 553540; BD Pharmingen), FITC anti-mouse IgMa (DS-1, IgG1; Catalog no. 553516; BD Pharmingen), PE anti-CD45R/B220 (RA3–6B2, IgG2a; Catalog no. 553090; BD Pharmingen), Goat anti-humanIgM F(ab′)2 (Catalog no. 109-006-129; Jackson ImmunoResearch), Rabbit anti-human IgM-btn (Catalog no. 309-065-095; Jackson ImmunoResearch), Biotin anti-mouse IgMa (Catalog no. 553515; BD Pharmingen), Anti-Mouse IgMb -btn(AF6–78, catlog no.553519; BD Pharmingen), Cholera Toxin B (CTB, catalog no. 227039; Calbiochem), Horseradish peroxidase conjugated (HRP-CTB, catalog no. C3741; Sigma), PP1 and PP2 (Src Family Tyrosine Kinase Inhibitor, catalog no. BML-EI275 and BML-EI297 respectively; Enzo Life Sciences), anti-GAPDH, mouse monoclonal 6C5 (catalog no. AM4300; Ambion). .. Cells were cultured in bicarbonate-free RPMI 1640 media (Sigma ) supplemented with 10% FBS and 50 μM 2-mercaptoethanol.

    Electron Microscopy:

    Article Title: Cis and Trans Regulatory Mechanisms Control AP2-Mediated B Cell Receptor Endocytosis via Select Tyrosine-Based Motifs
    Article Snippet: .. In brief, A20µWT cells or B10.Br-derived splenocytes were stimulated with rabbit anti-human IgM-btn (309-065-095; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA) or mouse anti-mouse IgMb -btn (AF6-78; BD Pharmingen, San Jose, CA, USA), respectively, plus goat anti-btn-15 nm gold (25253; Electron Microscopy Services [EMS], Hatfield, PA, USA) for 5 minutes at 37o C, then cooled. .. The stained cells were then pressed between alcian blue-coated coverslips and lysine-coated nickel EM grids (FCF300-Ni-50; EMS) and quickly separated.

    Article Title: The Syk-binding Ubiquitin Ligase c-Cbl Mediates Signaling-dependent B Cell Receptor Ubiquitination and B Cell Receptor-mediated Antigen Processing and Presentation *
    Article Snippet: .. The following reagents were used for this study: hen egg lysozyme (HEL, catalog no. l -6876, Sigma), HEL46–61 -I-Ak -specific monoclonal antibody C4H3 , goat anti-mouse IgG F(ab′)2 (catalog no. 115-006-006, Jackson ImmunoResearch, West Grove, PA), FITC rat anti-mouse IgG1 (A85-1, catalog no. 553443, BD Biosciences), FITC anti-mouse I-Ak (10-3.6, IgG2a , catalog no. 55352, BD Biosciences), FITC anti-mouse IgMa (DS-1, IgG1 , catalog no. 553516, BD Biosciences), PE anti-CD45R/B220 (RA3-6B2, IgG2a , catalog no. 553090, BD Biosciences), goat anti-human IgM F(ab′)2 (catalog no. 109-006-129, Jackson ImmunoResearch), rabbit anti-human IgM-btn (catalog no. 309-065-095, Jackson ImmunoResearch), biotin anti-mouse IgMa (catalog no. 553515, BD Biosciences), c-Cbl antibody (catalog no. 2747, Cell Signaling Technology), purified mouse anti-c-Cbl (catalog no. 610442, BD Biosciences), Cbl-b (clone G1, catalog no. sc-8006), Cbl-b (clone C20, catalogue no. sc-1435), purified rat anti-mouse CD107b (LAMP-2) monoclonal antibody (BD Biosciences, catalog no. 558756), SA-Alexa Fluor 594 (Molecular Probes, catalog no. ); Alexa Fluor 647 anti-rat IgG (H+L) (Molecular Probes, catalog no. A-21247), fluoro-gel (Electron Microscopy Sciences, catalog no. 17985-10), RNeasy mini kit (Qiagen, catalog no. 74104), Transcriptor First Strand cDNA synthesis kit (Roche, catalog no. 04 379 012 001), and AccuPrime TaqDNA polymerase (Invitrogen, catalog no. 12339016). ..

    Purification:

    Article Title: The Syk-binding Ubiquitin Ligase c-Cbl Mediates Signaling-dependent B Cell Receptor Ubiquitination and B Cell Receptor-mediated Antigen Processing and Presentation *
    Article Snippet: .. The following reagents were used for this study: hen egg lysozyme (HEL, catalog no. l -6876, Sigma), HEL46–61 -I-Ak -specific monoclonal antibody C4H3 , goat anti-mouse IgG F(ab′)2 (catalog no. 115-006-006, Jackson ImmunoResearch, West Grove, PA), FITC rat anti-mouse IgG1 (A85-1, catalog no. 553443, BD Biosciences), FITC anti-mouse I-Ak (10-3.6, IgG2a , catalog no. 55352, BD Biosciences), FITC anti-mouse IgMa (DS-1, IgG1 , catalog no. 553516, BD Biosciences), PE anti-CD45R/B220 (RA3-6B2, IgG2a , catalog no. 553090, BD Biosciences), goat anti-human IgM F(ab′)2 (catalog no. 109-006-129, Jackson ImmunoResearch), rabbit anti-human IgM-btn (catalog no. 309-065-095, Jackson ImmunoResearch), biotin anti-mouse IgMa (catalog no. 553515, BD Biosciences), c-Cbl antibody (catalog no. 2747, Cell Signaling Technology), purified mouse anti-c-Cbl (catalog no. 610442, BD Biosciences), Cbl-b (clone G1, catalog no. sc-8006), Cbl-b (clone C20, catalogue no. sc-1435), purified rat anti-mouse CD107b (LAMP-2) monoclonal antibody (BD Biosciences, catalog no. 558756), SA-Alexa Fluor 594 (Molecular Probes, catalog no. ); Alexa Fluor 647 anti-rat IgG (H+L) (Molecular Probes, catalog no. A-21247), fluoro-gel (Electron Microscopy Sciences, catalog no. 17985-10), RNeasy mini kit (Qiagen, catalog no. 74104), Transcriptor First Strand cDNA synthesis kit (Roche, catalog no. 04 379 012 001), and AccuPrime TaqDNA polymerase (Invitrogen, catalog no. 12339016). ..

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  • About
  • News
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  • 85
    Jackson Immuno biotin sp affinipure rabbit anti human igm fc5µ fragment specific
    c-Cbl mediates Ag·BCR ubiquitination and processing. Upper panel , B cells were pulsed with anti-human <t>IgM-btn</t> for the indicated time (minutes) at 37 °C. The cells were lysed and ubiquitinated ligand-BCR complexes isolated by ubiquitin
    Biotin Sp Affinipure Rabbit Anti Human Igm Fc5µ Fragment Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp affinipure rabbit anti human igm fc5µ fragment specific/product/Jackson Immuno
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure rabbit anti human igm fc5µ fragment specific - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    Image Search Results


    c-Cbl mediates Ag·BCR ubiquitination and processing. Upper panel , B cells were pulsed with anti-human IgM-btn for the indicated time (minutes) at 37 °C. The cells were lysed and ubiquitinated ligand-BCR complexes isolated by ubiquitin

    Journal: The Journal of Biological Chemistry

    Article Title: The Syk-binding Ubiquitin Ligase c-Cbl Mediates Signaling-dependent B Cell Receptor Ubiquitination and B Cell Receptor-mediated Antigen Processing and Presentation *

    doi: 10.1074/jbc.M112.357640

    Figure Lengend Snippet: c-Cbl mediates Ag·BCR ubiquitination and processing. Upper panel , B cells were pulsed with anti-human IgM-btn for the indicated time (minutes) at 37 °C. The cells were lysed and ubiquitinated ligand-BCR complexes isolated by ubiquitin

    Article Snippet: The following reagents were used for this study: hen egg lysozyme (HEL, catalog no. l -6876, Sigma), HEL46–61 -I-Ak -specific monoclonal antibody C4H3 , goat anti-mouse IgG F(ab′)2 (catalog no. 115-006-006, Jackson ImmunoResearch, West Grove, PA), FITC rat anti-mouse IgG1 (A85-1, catalog no. 553443, BD Biosciences), FITC anti-mouse I-Ak (10-3.6, IgG2a , catalog no. 55352, BD Biosciences), FITC anti-mouse IgMa (DS-1, IgG1 , catalog no. 553516, BD Biosciences), PE anti-CD45R/B220 (RA3-6B2, IgG2a , catalog no. 553090, BD Biosciences), goat anti-human IgM F(ab′)2 (catalog no. 109-006-129, Jackson ImmunoResearch), rabbit anti-human IgM-btn (catalog no. 309-065-095, Jackson ImmunoResearch), biotin anti-mouse IgMa (catalog no. 553515, BD Biosciences), c-Cbl antibody (catalog no. 2747, Cell Signaling Technology), purified mouse anti-c-Cbl (catalog no. 610442, BD Biosciences), Cbl-b (clone G1, catalog no. sc-8006), Cbl-b (clone C20, catalogue no. sc-1435), purified rat anti-mouse CD107b (LAMP-2) monoclonal antibody (BD Biosciences, catalog no. 558756), SA-Alexa Fluor 594 (Molecular Probes, catalog no. ); Alexa Fluor 647 anti-rat IgG (H+L) (Molecular Probes, catalog no. A-21247), fluoro-gel (Electron Microscopy Sciences, catalog no. 17985-10), RNeasy mini kit (Qiagen, catalog no. 74104), Transcriptor First Strand cDNA synthesis kit (Roche, catalog no. 04 379 012 001), and AccuPrime TaqDNA polymerase (Invitrogen, catalog no. 12339016).

    Techniques: Isolation

    Ultrastructural Colocalization of Ligand-BCR Complexes and AP2 in Clathrin-Coated Pits. A20µWT B cells or primary murine splenocytes were pulsed with anti-IgM-btn followed by anti-biotin-15 nm gold (arrow heads), incubated 2 minutes at 37° and then plasma membrane rips were prepared as previously reported [10] . The exposed cytoplasmic face of the plasma membrane was stained with anti-AP2 and Protein A-5 nm gold (arrows). The percent of BCR-containing CCP that also stained for AP2 is indicated below each image. Inset: AP2 and BCR co-localization within electron dense membrane regions lacking discernable CCP architecture. Shown are representative images from 1 of 3 experiments, with 1,000+ BCR-bound gold particles or 100+ CCP photographed cumulatively.

    Journal: PLoS ONE

    Article Title: Cis and Trans Regulatory Mechanisms Control AP2-Mediated B Cell Receptor Endocytosis via Select Tyrosine-Based Motifs

    doi: 10.1371/journal.pone.0054938

    Figure Lengend Snippet: Ultrastructural Colocalization of Ligand-BCR Complexes and AP2 in Clathrin-Coated Pits. A20µWT B cells or primary murine splenocytes were pulsed with anti-IgM-btn followed by anti-biotin-15 nm gold (arrow heads), incubated 2 minutes at 37° and then plasma membrane rips were prepared as previously reported [10] . The exposed cytoplasmic face of the plasma membrane was stained with anti-AP2 and Protein A-5 nm gold (arrows). The percent of BCR-containing CCP that also stained for AP2 is indicated below each image. Inset: AP2 and BCR co-localization within electron dense membrane regions lacking discernable CCP architecture. Shown are representative images from 1 of 3 experiments, with 1,000+ BCR-bound gold particles or 100+ CCP photographed cumulatively.

    Article Snippet: In brief, A20µWT cells or B10.Br-derived splenocytes were stimulated with rabbit anti-human IgM-btn (309-065-095; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA) or mouse anti-mouse IgMb -btn (AF6-78; BD Pharmingen, San Jose, CA, USA), respectively, plus goat anti-btn-15 nm gold (25253; Electron Microscopy Services [EMS], Hatfield, PA, USA) for 5 minutes at 37o C, then cooled.

    Techniques: Incubation, Staining