biotin sp affinipure rabbit anti goat igg  (Jackson Immuno)

 
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  • 99
    Name:
    Biotin SP AffiniPure Rabbit Anti Goat IgG
    Description:
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule goat IgG It also reacts with the light chains of other goat immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    Catalog Number:
    305-065-003
    Price:
    116
    Purity:
    The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
    Conjugate:
    Biotin SP long spacer
    Size:
    ml
    Category:
    Secondary Antibody
    Source:
    Rabbit
    Quantity:
    1 5
    Buy from Supplier


    Structured Review

    Jackson Immuno biotin sp affinipure rabbit anti goat igg
    Biotin SP AffiniPure Rabbit Anti Goat IgG
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule goat IgG It also reacts with the light chains of other goat immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    https://www.bioz.com/result/biotin sp affinipure rabbit anti goat igg/product/Jackson Immuno
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure rabbit anti goat igg - by Bioz Stars, 2020-04
    99/100 stars

    Related Products / Commonly Used Together

    biotin-sp-affinipure rabbit

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    Related Articles

    Blocking Assay:

    Article Title: Honokiol suppresses proliferation and induces apoptosis via regulation of the miR-21/PTEN/PI3K/AKT signaling pathway in human osteosarcoma cells
    Article Snippet: After blocking with 5% non-fat milk at room temperature for 1 h, PVDF membranes were incubated with primary antibodies (dilution 1:500) for 2 h at room temperature and followed with a horseradish peroxidase conjugated secondary antibody (dilution 1:1,000) for 1 h at room temperature. .. The corresponding rabbit anti-mouse (315-065-003) and goat anti-rabbit (305-065-003) secondary antibodies were purchased from Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA, USA).

    Incubation:

    Article Title: Honokiol suppresses proliferation and induces apoptosis via regulation of the miR-21/PTEN/PI3K/AKT signaling pathway in human osteosarcoma cells
    Article Snippet: After blocking with 5% non-fat milk at room temperature for 1 h, PVDF membranes were incubated with primary antibodies (dilution 1:500) for 2 h at room temperature and followed with a horseradish peroxidase conjugated secondary antibody (dilution 1:1,000) for 1 h at room temperature. .. The corresponding rabbit anti-mouse (315-065-003) and goat anti-rabbit (305-065-003) secondary antibodies were purchased from Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA, USA).

    Western Blot:

    Article Title: Honokiol suppresses proliferation and induces apoptosis via regulation of the miR-21/PTEN/PI3K/AKT signaling pathway in human osteosarcoma cells
    Article Snippet: Paragraph title: Western blot analysis ... The corresponding rabbit anti-mouse (315-065-003) and goat anti-rabbit (305-065-003) secondary antibodies were purchased from Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA, USA).

    SDS Page:

    Article Title: Honokiol suppresses proliferation and induces apoptosis via regulation of the miR-21/PTEN/PI3K/AKT signaling pathway in human osteosarcoma cells
    Article Snippet: Total proteins (60 µ g) were separated by 10% SDS-PAGE (Sigma-Aldrich; Merck KGaA) and were then transferred onto polyvinylidene difluoride (PVDF) membranes (BD Biosciences). .. The corresponding rabbit anti-mouse (315-065-003) and goat anti-rabbit (305-065-003) secondary antibodies were purchased from Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA, USA).

    Software:

    Article Title: Honokiol suppresses proliferation and induces apoptosis via regulation of the miR-21/PTEN/PI3K/AKT signaling pathway in human osteosarcoma cells
    Article Snippet: The corresponding rabbit anti-mouse (315-065-003) and goat anti-rabbit (305-065-003) secondary antibodies were purchased from Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA, USA). .. AlphaImager software version 2000 (ProteinSimple, San Jose, CA, USA) was used to measure relative intensity of each band on the blots.

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  • 87
    Jackson Immuno cd106
    Candidate cell surface markers are differentially expressed in MB cell lines/primary cultures and patient samples A. Representative dot plots of staining for candidate biomarkers in Daoy, MED311-FH and UI226 tumorspheres (SHH variant) vs . tumorspheres from Group 3/Group 4 cell lines by flow cytometry. Insets: respective isotype controls. 7AAD: 7-aminoactinomycin D cell viability dye. B. - D. CD271 (B), CD171 (C) and <t>CD106</t> (D) expression in paraffin embedded sections of fetal cerebellum and primary medulloblastoma samples. Scale bar: 400 μm.
    Cd106, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd106/product/Jackson Immuno
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd106 - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

    88
    Jackson Immuno biotin sp conjugated affinipure goat anti rabbit igg
    Candidate cell surface markers are differentially expressed in MB cell lines/primary cultures and patient samples A. Representative dot plots of staining for candidate biomarkers in Daoy, MED311-FH and UI226 tumorspheres (SHH variant) vs . tumorspheres from Group 3/Group 4 cell lines by flow cytometry. Insets: respective isotype controls. 7AAD: 7-aminoactinomycin D cell viability dye. B. - D. CD271 (B), CD171 (C) and <t>CD106</t> (D) expression in paraffin embedded sections of fetal cerebellum and primary medulloblastoma samples. Scale bar: 400 μm.
    Biotin Sp Conjugated Affinipure Goat Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp conjugated affinipure goat anti rabbit igg/product/Jackson Immuno
    Average 88 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    biotin sp conjugated affinipure goat anti rabbit igg - by Bioz Stars, 2020-04
    88/100 stars
      Buy from Supplier

    Image Search Results


    Candidate cell surface markers are differentially expressed in MB cell lines/primary cultures and patient samples A. Representative dot plots of staining for candidate biomarkers in Daoy, MED311-FH and UI226 tumorspheres (SHH variant) vs . tumorspheres from Group 3/Group 4 cell lines by flow cytometry. Insets: respective isotype controls. 7AAD: 7-aminoactinomycin D cell viability dye. B. - D. CD271 (B), CD171 (C) and CD106 (D) expression in paraffin embedded sections of fetal cerebellum and primary medulloblastoma samples. Scale bar: 400 μm.

    Journal: Oncotarget

    Article Title: Characterization of novel biomarkers in selecting for subtype specific medulloblastoma phenotypes

    doi:

    Figure Lengend Snippet: Candidate cell surface markers are differentially expressed in MB cell lines/primary cultures and patient samples A. Representative dot plots of staining for candidate biomarkers in Daoy, MED311-FH and UI226 tumorspheres (SHH variant) vs . tumorspheres from Group 3/Group 4 cell lines by flow cytometry. Insets: respective isotype controls. 7AAD: 7-aminoactinomycin D cell viability dye. B. - D. CD271 (B), CD171 (C) and CD106 (D) expression in paraffin embedded sections of fetal cerebellum and primary medulloblastoma samples. Scale bar: 400 μm.

    Article Snippet: Slides were washed in (CD271): 1XPBS, (CD171/CD106): 1XTBS and treated with secondary antibody: for CD271 (1:500), for CD106 (1:200) (Biotin-SP-Affinipure Goat Anti-Rabbit IgG (Jackson Immunoresearch, West Grove, PA, USA) and for CD171 (1:200) (Biotin-SP-AffiniPure Sheep Anti-Mouse IgG (H+L) (Jackson Immunoresearch) diluted in (CD271): 1% lamb serum in 1XPBS, (CD171/CD106): 1% goat serum / 1% BSA diluted in TBS for 2 hours at room temperature.

    Techniques: Staining, Variant Assay, Flow Cytometry, Cytometry, Expressing

    Transcript levels of CD271/p75NTR, CD171L1CAM, EGFR and CD106/VCAM1 across the 4 MB molecular variants Note that the CD271 Toronto dataset was previously published in Neoplasia, 15, Morrison et al., Deconstruction of Medulloblastoma cellular heterogeneity reveals differences between the most highly invasion and self-renewing phenotypes , 384-398, Copyright Elsevier (2013). A. - C. Gene expression data from 3 independent transcriptome datasets representing 548 patient samples showing downregulation of CD171/NCAM-L1 and relative enrichment of CD271/p75NTR, CD106/VCAM1, and EGFR, in SHH tumors compared with the other variants. Bars denote 1.5 interquartile range within each group. All subgroups were compared using a Kruskal-Wallis test for significance. Data are presented as log2 -transformed signal intensity. P

    Journal: Oncotarget

    Article Title: Characterization of novel biomarkers in selecting for subtype specific medulloblastoma phenotypes

    doi:

    Figure Lengend Snippet: Transcript levels of CD271/p75NTR, CD171L1CAM, EGFR and CD106/VCAM1 across the 4 MB molecular variants Note that the CD271 Toronto dataset was previously published in Neoplasia, 15, Morrison et al., Deconstruction of Medulloblastoma cellular heterogeneity reveals differences between the most highly invasion and self-renewing phenotypes , 384-398, Copyright Elsevier (2013). A. - C. Gene expression data from 3 independent transcriptome datasets representing 548 patient samples showing downregulation of CD171/NCAM-L1 and relative enrichment of CD271/p75NTR, CD106/VCAM1, and EGFR, in SHH tumors compared with the other variants. Bars denote 1.5 interquartile range within each group. All subgroups were compared using a Kruskal-Wallis test for significance. Data are presented as log2 -transformed signal intensity. P

    Article Snippet: Slides were washed in (CD271): 1XPBS, (CD171/CD106): 1XTBS and treated with secondary antibody: for CD271 (1:500), for CD106 (1:200) (Biotin-SP-Affinipure Goat Anti-Rabbit IgG (Jackson Immunoresearch, West Grove, PA, USA) and for CD171 (1:200) (Biotin-SP-AffiniPure Sheep Anti-Mouse IgG (H+L) (Jackson Immunoresearch) diluted in (CD271): 1% lamb serum in 1XPBS, (CD171/CD106): 1% goat serum / 1% BSA diluted in TBS for 2 hours at room temperature.

    Techniques: Expressing, Transformation Assay