biotin sp affinipure mouse anti goat igg  (Jackson Immuno)

 
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    Name:
    Biotin SP AffiniPure Mouse Anti Goat IgG
    Description:
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule goat IgG It also reacts with the light chains of other goat immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody exhibits inherent minimal cross reaction to mouse serum proteins and has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with mouse human and rabbit serum proteins The antibody may cross react with immunoglobulins from other species
    Catalog Number:
    205-065-108
    Price:
    137
    Purity:
    The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
    Conjugate:
    Biotin SP long spacer
    Size:
    ml
    Category:
    Secondary Antibody
    Source:
    Mouse
    Quantity:
    1 0
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    Structured Review

    Jackson Immuno biotin sp affinipure mouse anti goat igg
    Biotin SP AffiniPure Mouse Anti Goat IgG
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule goat IgG It also reacts with the light chains of other goat immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody exhibits inherent minimal cross reaction to mouse serum proteins and has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with mouse human and rabbit serum proteins The antibody may cross react with immunoglobulins from other species
    https://www.bioz.com/result/biotin sp affinipure mouse anti goat igg/product/Jackson Immuno
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure mouse anti goat igg - by Bioz Stars, 2020-04
    92/100 stars

    Related Products / Commonly Used Together

    biotinylated goat anti-mouse immunoglobulins

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    Related Articles

    Staining:

    Article Title: Expression of growth factor receptors and targeting of EGFR in cholangiocarcinoma cell lines
    Article Snippet: Biotinylated goat anti-mouse immunoglobulins (#E0433, DakoCytomation) or biotin-SP-conjugated mouse anti-goat-IgG antibodies (#205-065-108, Jackson ImmunoResearch, Newmarket, England) were used as secondary antibodies. .. EGFR, HGFR, and IGF2R were stained by the standard ABC procedure.

    Immunohistochemistry:

    Article Title: Expression of growth factor receptors and targeting of EGFR in cholangiocarcinoma cell lines
    Article Snippet: Paragraph title: Immunohistochemistry (IHC) ... Biotinylated goat anti-mouse immunoglobulins (#E0433, DakoCytomation) or biotin-SP-conjugated mouse anti-goat-IgG antibodies (#205-065-108, Jackson ImmunoResearch, Newmarket, England) were used as secondary antibodies.

    Cell Culture:

    Article Title: Expression of growth factor receptors and targeting of EGFR in cholangiocarcinoma cell lines
    Article Snippet: Biotinylated goat anti-mouse immunoglobulins (#E0433, DakoCytomation) or biotin-SP-conjugated mouse anti-goat-IgG antibodies (#205-065-108, Jackson ImmunoResearch, Newmarket, England) were used as secondary antibodies. .. For IHC analysis, cells were cultured in 4-chamber slides for 3-4 days, and fixed with 3.7% formaldehyde for 15 min. IGF1R was studied with the APAAP method.

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  • 92
    Jackson Immuno biotin sp conjugated affinipure goat anti mouse
    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated <t>AffiniPure</t> goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p
    Biotin Sp Conjugated Affinipure Goat Anti Mouse, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp conjugated affinipure goat anti mouse/product/Jackson Immuno
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    biotin sp conjugated affinipure goat anti mouse - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    99
    Jackson Immuno biotin sp affinipure goat anti mouse igg f ab 2 fragment specific
    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated <t>AffiniPure</t> goat anti-mouse <t>IgG,F(ab′)2</t> fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p
    Biotin Sp Affinipure Goat Anti Mouse Igg F Ab 2 Fragment Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp affinipure goat anti mouse igg f ab 2 fragment specific/product/Jackson Immuno
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure goat anti mouse igg f ab 2 fragment specific - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Jackson Immuno igm
    DRibbles derived from tumor cells induce specific antibody production in vivo. (A and B) Serum was collected from C57/BL6 mice at day 7 after first intravenous injection of PBS or Hep1-6 derived DRibbles (DRs). The total <t>IgM</t> (A) and <t>IgG</t> (B) in serum was measured by ELISA. Values are the mean ± SEM derived from three mice per group (n = 3). (C and D) Hep1-6 or B16F10 cells were incubated for 1 hour with serum derived from PBS or Hep1-6 DRibbles injected mice respectively. After washing, Hep1-6 or B16F10 cells were incubated with FITC-labeled anti-mouse IgM antibody (C) or FITC-labeled anti-mouse IgG antibody (D). After washing, the cells were analyzed by flow cytometry. (E and F) HepI-6 or B16F10 cells were treated as C and D, and then the cells were stained with DAPI and analyzed by fluorescence microscope. Green fluorescence represents FITC-labeled anti-mouse IgM antibody (E) or FITC-labeled anti-mouse IgG antibody (F). Blue fluorescence represents the cell nucleus. A representative of three independent experiments was showed.
    Igm, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igm/product/Jackson Immuno
    Average 99 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    igm - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p

    Journal: PLoS ONE

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis

    doi: 10.1371/journal.pone.0109352

    Figure Lengend Snippet: Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p

    Article Snippet: After washing, the cells were stained with biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072), followed by PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) and CD56-FITC.

    Techniques: Flow Cytometry, Cytometry, Cell Culture, Staining, Irradiation

    Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.

    Journal: PLoS ONE

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis

    doi: 10.1371/journal.pone.0109352

    Figure Lengend Snippet: Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.

    Article Snippet: After washing, the cells were stained with biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072), followed by PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) and CD56-FITC.

    Techniques: Immunofluorescence, Staining, Cell Culture

    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p

    Journal: PLoS ONE

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis

    doi: 10.1371/journal.pone.0109352

    Figure Lengend Snippet: Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p

    Article Snippet: Biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072) and PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) were used for labeling the cells.

    Techniques: Flow Cytometry, Cytometry, Cell Culture, Staining, Irradiation

    Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.

    Journal: PLoS ONE

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis

    doi: 10.1371/journal.pone.0109352

    Figure Lengend Snippet: Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.

    Article Snippet: Biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072) and PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) were used for labeling the cells.

    Techniques: Immunofluorescence, Staining, Cell Culture

    DRibbles derived from tumor cells induce specific antibody production in vivo. (A and B) Serum was collected from C57/BL6 mice at day 7 after first intravenous injection of PBS or Hep1-6 derived DRibbles (DRs). The total IgM (A) and IgG (B) in serum was measured by ELISA. Values are the mean ± SEM derived from three mice per group (n = 3). (C and D) Hep1-6 or B16F10 cells were incubated for 1 hour with serum derived from PBS or Hep1-6 DRibbles injected mice respectively. After washing, Hep1-6 or B16F10 cells were incubated with FITC-labeled anti-mouse IgM antibody (C) or FITC-labeled anti-mouse IgG antibody (D). After washing, the cells were analyzed by flow cytometry. (E and F) HepI-6 or B16F10 cells were treated as C and D, and then the cells were stained with DAPI and analyzed by fluorescence microscope. Green fluorescence represents FITC-labeled anti-mouse IgM antibody (E) or FITC-labeled anti-mouse IgG antibody (F). Blue fluorescence represents the cell nucleus. A representative of three independent experiments was showed.

    Journal: PLoS ONE

    Article Title: Tumor-Derived Autophagosomes (DRibbles) Induce B Cell Activation in a TLR2-MyD88 Dependent Manner

    doi: 10.1371/journal.pone.0053564

    Figure Lengend Snippet: DRibbles derived from tumor cells induce specific antibody production in vivo. (A and B) Serum was collected from C57/BL6 mice at day 7 after first intravenous injection of PBS or Hep1-6 derived DRibbles (DRs). The total IgM (A) and IgG (B) in serum was measured by ELISA. Values are the mean ± SEM derived from three mice per group (n = 3). (C and D) Hep1-6 or B16F10 cells were incubated for 1 hour with serum derived from PBS or Hep1-6 DRibbles injected mice respectively. After washing, Hep1-6 or B16F10 cells were incubated with FITC-labeled anti-mouse IgM antibody (C) or FITC-labeled anti-mouse IgG antibody (D). After washing, the cells were analyzed by flow cytometry. (E and F) HepI-6 or B16F10 cells were treated as C and D, and then the cells were stained with DAPI and analyzed by fluorescence microscope. Green fluorescence represents FITC-labeled anti-mouse IgM antibody (E) or FITC-labeled anti-mouse IgG antibody (F). Blue fluorescence represents the cell nucleus. A representative of three independent experiments was showed.

    Article Snippet: Briefly, The plates were coated with either goat anti-mouse IgG or IgM (Jackson Immuno Research Laboratories) at 1∶10000 overnight at 4°C and washed with ELISA washing buffer (PBS plus 0.5% Tween-20).

    Techniques: Derivative Assay, In Vivo, Mouse Assay, Injection, Enzyme-linked Immunosorbent Assay, Incubation, Labeling, Flow Cytometry, Cytometry, Staining, Fluorescence, Microscopy