Structured Review

Jackson Immuno biotin sp affinipure goat anti rat igg
Biotin Sp Affinipure Goat Anti Rat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotin sp affinipure goat anti rat igg/product/Jackson Immuno
Average 96 stars, based on 10 article reviews
Price from $9.99 to $1999.99
biotin sp affinipure goat anti rat igg - by Bioz Stars, 2020-04
96/100 stars

Images

Related Articles

Immunohistochemistry:

Article Title: Selective Enhancement of Dopamine Release in the Ventral Pallidum of Methamphetamine-Sensitized Mice
Article Snippet: Paragraph title: Immunohistochemistry ... Sections were incubated with polyclonal rabbit anti-SV2C serum (Covance, 1:2500), rat anti-DAT (Millipore MAB369, 1:1000), mouse anti-TH (Millipore MAB318, 1:1000), or rabbit anti-VMAT2 (generated in our laboratory, 1:10000) followed by biotinylated secondary antibody (Jackson ImmunoResearch: goat anti-rabbit biotin 111-065-144, goat anti-mouse biotin 115-065-003, goat anti-rat 112-065-003).

Article Title: Acute Kahweol Treatment Attenuates Traumatic Brain Injury Neuroinflammation and Functional Deficits
Article Snippet: .. For CD45 immunohistochemical staining, sections were rehydrated and quenched with 0.3% H2 O2 for 10 min. After blocking with 10% normal goat serum (Jackson Immuno Research Inc., Bellefonte, PA, USA), sections were incubated with anti-mouse CD45 primary antibody (550539, BD pharmingen, San Diego, CA, USA) overnight at 4 °C, followed by goat anti-rat (1:1000; 112-065-003, Jackson Immuno Research Inc., Bellefonte, PA, USA) secondary antibody incubation for 2 h at room temperature. .. The signal of the secondary antibody was amplified with VECTASTAIN Elite ABC Kit (Vector Laboratories, Carlsbad, CA, USA).

Amplification:

Article Title: Acute Kahweol Treatment Attenuates Traumatic Brain Injury Neuroinflammation and Functional Deficits
Article Snippet: For CD45 immunohistochemical staining, sections were rehydrated and quenched with 0.3% H2 O2 for 10 min. After blocking with 10% normal goat serum (Jackson Immuno Research Inc., Bellefonte, PA, USA), sections were incubated with anti-mouse CD45 primary antibody (550539, BD pharmingen, San Diego, CA, USA) overnight at 4 °C, followed by goat anti-rat (1:1000; 112-065-003, Jackson Immuno Research Inc., Bellefonte, PA, USA) secondary antibody incubation for 2 h at room temperature. .. The signal of the secondary antibody was amplified with VECTASTAIN Elite ABC Kit (Vector Laboratories, Carlsbad, CA, USA).

Fluorescence:

Article Title: Acute Kahweol Treatment Attenuates Traumatic Brain Injury Neuroinflammation and Functional Deficits
Article Snippet: For CD45 immunohistochemical staining, sections were rehydrated and quenched with 0.3% H2 O2 for 10 min. After blocking with 10% normal goat serum (Jackson Immuno Research Inc., Bellefonte, PA, USA), sections were incubated with anti-mouse CD45 primary antibody (550539, BD pharmingen, San Diego, CA, USA) overnight at 4 °C, followed by goat anti-rat (1:1000; 112-065-003, Jackson Immuno Research Inc., Bellefonte, PA, USA) secondary antibody incubation for 2 h at room temperature. .. For CD45 immunohistochemical staining, sections were rehydrated and quenched with 0.3% H2 O2 for 10 min. After blocking with 10% normal goat serum (Jackson Immuno Research Inc., Bellefonte, PA, USA), sections were incubated with anti-mouse CD45 primary antibody (550539, BD pharmingen, San Diego, CA, USA) overnight at 4 °C, followed by goat anti-rat (1:1000; 112-065-003, Jackson Immuno Research Inc., Bellefonte, PA, USA) secondary antibody incubation for 2 h at room temperature.

Isolation:

Article Title: Selective Enhancement of Dopamine Release in the Ventral Pallidum of Methamphetamine-Sensitized Mice
Article Snippet: Polyclonal anti-SV2C serum was isolated from rabbits injected with an N-terminal peptide (amino acids 97–114: STNQGKDSIVSVGQPKG) conjugated to Imject maleimide activated mcKLH (Thermo Scientific), and sera were generated for our laboratory using Covance Custom Immunology Services. .. Sections were incubated with polyclonal rabbit anti-SV2C serum (Covance, 1:2500), rat anti-DAT (Millipore MAB369, 1:1000), mouse anti-TH (Millipore MAB318, 1:1000), or rabbit anti-VMAT2 (generated in our laboratory, 1:10000) followed by biotinylated secondary antibody (Jackson ImmunoResearch: goat anti-rabbit biotin 111-065-144, goat anti-mouse biotin 115-065-003, goat anti-rat 112-065-003).

Incubation:

Article Title: Selective Enhancement of Dopamine Release in the Ventral Pallidum of Methamphetamine-Sensitized Mice
Article Snippet: .. Sections were incubated with polyclonal rabbit anti-SV2C serum (Covance, 1:2500), rat anti-DAT (Millipore MAB369, 1:1000), mouse anti-TH (Millipore MAB318, 1:1000), or rabbit anti-VMAT2 (generated in our laboratory, 1:10000) followed by biotinylated secondary antibody (Jackson ImmunoResearch: goat anti-rabbit biotin 111-065-144, goat anti-mouse biotin 115-065-003, goat anti-rat 112-065-003). .. Visualization was performed with a 3,3-diaminobenzidine (DAB) reaction (Vector Laboratories) for biotinylated secondary antibodies.

Article Title: Acute Kahweol Treatment Attenuates Traumatic Brain Injury Neuroinflammation and Functional Deficits
Article Snippet: .. For CD45 immunohistochemical staining, sections were rehydrated and quenched with 0.3% H2 O2 for 10 min. After blocking with 10% normal goat serum (Jackson Immuno Research Inc., Bellefonte, PA, USA), sections were incubated with anti-mouse CD45 primary antibody (550539, BD pharmingen, San Diego, CA, USA) overnight at 4 °C, followed by goat anti-rat (1:1000; 112-065-003, Jackson Immuno Research Inc., Bellefonte, PA, USA) secondary antibody incubation for 2 h at room temperature. .. The signal of the secondary antibody was amplified with VECTASTAIN Elite ABC Kit (Vector Laboratories, Carlsbad, CA, USA).

Microscopy:

Article Title: Selective Enhancement of Dopamine Release in the Ventral Pallidum of Methamphetamine-Sensitized Mice
Article Snippet: Sections were incubated with polyclonal rabbit anti-SV2C serum (Covance, 1:2500), rat anti-DAT (Millipore MAB369, 1:1000), mouse anti-TH (Millipore MAB318, 1:1000), or rabbit anti-VMAT2 (generated in our laboratory, 1:10000) followed by biotinylated secondary antibody (Jackson ImmunoResearch: goat anti-rabbit biotin 111-065-144, goat anti-mouse biotin 115-065-003, goat anti-rat 112-065-003). .. Images were acquired with a NeuroLucida epifluorescent microscope (MicroBrightField).

Article Title: Acute Kahweol Treatment Attenuates Traumatic Brain Injury Neuroinflammation and Functional Deficits
Article Snippet: For CD45 immunohistochemical staining, sections were rehydrated and quenched with 0.3% H2 O2 for 10 min. After blocking with 10% normal goat serum (Jackson Immuno Research Inc., Bellefonte, PA, USA), sections were incubated with anti-mouse CD45 primary antibody (550539, BD pharmingen, San Diego, CA, USA) overnight at 4 °C, followed by goat anti-rat (1:1000; 112-065-003, Jackson Immuno Research Inc., Bellefonte, PA, USA) secondary antibody incubation for 2 h at room temperature. .. For CD45 immunohistochemical staining, sections were rehydrated and quenched with 0.3% H2 O2 for 10 min. After blocking with 10% normal goat serum (Jackson Immuno Research Inc., Bellefonte, PA, USA), sections were incubated with anti-mouse CD45 primary antibody (550539, BD pharmingen, San Diego, CA, USA) overnight at 4 °C, followed by goat anti-rat (1:1000; 112-065-003, Jackson Immuno Research Inc., Bellefonte, PA, USA) secondary antibody incubation for 2 h at room temperature.

Activation Assay:

Article Title: Acute Kahweol Treatment Attenuates Traumatic Brain Injury Neuroinflammation and Functional Deficits
Article Snippet: Paragraph title: 2.7. Measurements of Degenerating Neurons, Macrophage/Microglia Activation, and Neutrophil and Leukocyte Infiltration ... For CD45 immunohistochemical staining, sections were rehydrated and quenched with 0.3% H2 O2 for 10 min. After blocking with 10% normal goat serum (Jackson Immuno Research Inc., Bellefonte, PA, USA), sections were incubated with anti-mouse CD45 primary antibody (550539, BD pharmingen, San Diego, CA, USA) overnight at 4 °C, followed by goat anti-rat (1:1000; 112-065-003, Jackson Immuno Research Inc., Bellefonte, PA, USA) secondary antibody incubation for 2 h at room temperature.

Generated:

Article Title: Selective Enhancement of Dopamine Release in the Ventral Pallidum of Methamphetamine-Sensitized Mice
Article Snippet: .. Sections were incubated with polyclonal rabbit anti-SV2C serum (Covance, 1:2500), rat anti-DAT (Millipore MAB369, 1:1000), mouse anti-TH (Millipore MAB318, 1:1000), or rabbit anti-VMAT2 (generated in our laboratory, 1:10000) followed by biotinylated secondary antibody (Jackson ImmunoResearch: goat anti-rabbit biotin 111-065-144, goat anti-mouse biotin 115-065-003, goat anti-rat 112-065-003). .. Visualization was performed with a 3,3-diaminobenzidine (DAB) reaction (Vector Laboratories) for biotinylated secondary antibodies.

other:

Article Title: Galectin-7 in the Control of Epidermal Homeostasis after Injury
Article Snippet: Secondary Abs were as follows: Alexa488-conjugated goat anti-rabbit Ab (1:500, ; Invitrogen, Cergy Pontoise, France), Alexa568-conjugated goat anti-mouse Ab (1:500, ; Invitrogen); and biotinylated goat anti-rat Ab (1:500, 112-065-003; Jackson ImmunoResearch Laboratories, West Grove, PA) followed by Alexa568-conjugated streptavidin (1:500, S11226; Invitrogen).

Stripping Membranes:

Article Title: Dieldrin exposure induces oxidative damage in the mouse nigrostriatal dopamine system
Article Snippet: The source of immunochemical reagents is as follows: rat monoclonal antibody to DAT (cat #MAB369), rabbit polyclonal antibody to TH (cat #AB152), mouse monoclonal antibody to TH (cat #MAB318), rabbit polyclonal antibody to GAT-1 (cat #AB1570W), polyclonal guinea pig antibody to GLT (cat #AB783), and goat anti-guinea pig secondary antibody (cat #AP108P), Chemicon (Temecula, CA); monoclonal mouse antibody to α-tubulin (cat #T-9026), Sigma (St. Louis, MO); polyclonal mouse antibody to α-synuclein (cat #610787), BD Transduction (Bedford, MA); goat anti-rat (cat #112-065-143) and Cy3 goat anti-mouse (cat #115-165-166) secondary antibodies, Jackson ImmunoResearch (West Grove, PA); goat anti-rabbit (cat #170-6515) and goat anti-mouse (cat #170-5047) secondary antibodies, Bio-Rad (Hercules, CA). .. Super Signal West substrate and stripping buffer were obtained from Pierce (Rockford, IL).

Blocking Assay:

Article Title: Acute Kahweol Treatment Attenuates Traumatic Brain Injury Neuroinflammation and Functional Deficits
Article Snippet: .. For CD45 immunohistochemical staining, sections were rehydrated and quenched with 0.3% H2 O2 for 10 min. After blocking with 10% normal goat serum (Jackson Immuno Research Inc., Bellefonte, PA, USA), sections were incubated with anti-mouse CD45 primary antibody (550539, BD pharmingen, San Diego, CA, USA) overnight at 4 °C, followed by goat anti-rat (1:1000; 112-065-003, Jackson Immuno Research Inc., Bellefonte, PA, USA) secondary antibody incubation for 2 h at room temperature. .. The signal of the secondary antibody was amplified with VECTASTAIN Elite ABC Kit (Vector Laboratories, Carlsbad, CA, USA).

Staining:

Article Title: Acute Kahweol Treatment Attenuates Traumatic Brain Injury Neuroinflammation and Functional Deficits
Article Snippet: .. For CD45 immunohistochemical staining, sections were rehydrated and quenched with 0.3% H2 O2 for 10 min. After blocking with 10% normal goat serum (Jackson Immuno Research Inc., Bellefonte, PA, USA), sections were incubated with anti-mouse CD45 primary antibody (550539, BD pharmingen, San Diego, CA, USA) overnight at 4 °C, followed by goat anti-rat (1:1000; 112-065-003, Jackson Immuno Research Inc., Bellefonte, PA, USA) secondary antibody incubation for 2 h at room temperature. .. The signal of the secondary antibody was amplified with VECTASTAIN Elite ABC Kit (Vector Laboratories, Carlsbad, CA, USA).

Injection:

Article Title: Selective Enhancement of Dopamine Release in the Ventral Pallidum of Methamphetamine-Sensitized Mice
Article Snippet: Polyclonal anti-SV2C serum was isolated from rabbits injected with an N-terminal peptide (amino acids 97–114: STNQGKDSIVSVGQPKG) conjugated to Imject maleimide activated mcKLH (Thermo Scientific), and sera were generated for our laboratory using Covance Custom Immunology Services. .. Sections were incubated with polyclonal rabbit anti-SV2C serum (Covance, 1:2500), rat anti-DAT (Millipore MAB369, 1:1000), mouse anti-TH (Millipore MAB318, 1:1000), or rabbit anti-VMAT2 (generated in our laboratory, 1:10000) followed by biotinylated secondary antibody (Jackson ImmunoResearch: goat anti-rabbit biotin 111-065-144, goat anti-mouse biotin 115-065-003, goat anti-rat 112-065-003).

Plasmid Preparation:

Article Title: Selective Enhancement of Dopamine Release in the Ventral Pallidum of Methamphetamine-Sensitized Mice
Article Snippet: Sections were incubated with polyclonal rabbit anti-SV2C serum (Covance, 1:2500), rat anti-DAT (Millipore MAB369, 1:1000), mouse anti-TH (Millipore MAB318, 1:1000), or rabbit anti-VMAT2 (generated in our laboratory, 1:10000) followed by biotinylated secondary antibody (Jackson ImmunoResearch: goat anti-rabbit biotin 111-065-144, goat anti-mouse biotin 115-065-003, goat anti-rat 112-065-003). .. Sections were incubated with polyclonal rabbit anti-SV2C serum (Covance, 1:2500), rat anti-DAT (Millipore MAB369, 1:1000), mouse anti-TH (Millipore MAB318, 1:1000), or rabbit anti-VMAT2 (generated in our laboratory, 1:10000) followed by biotinylated secondary antibody (Jackson ImmunoResearch: goat anti-rabbit biotin 111-065-144, goat anti-mouse biotin 115-065-003, goat anti-rat 112-065-003).

Article Title: Acute Kahweol Treatment Attenuates Traumatic Brain Injury Neuroinflammation and Functional Deficits
Article Snippet: For CD45 immunohistochemical staining, sections were rehydrated and quenched with 0.3% H2 O2 for 10 min. After blocking with 10% normal goat serum (Jackson Immuno Research Inc., Bellefonte, PA, USA), sections were incubated with anti-mouse CD45 primary antibody (550539, BD pharmingen, San Diego, CA, USA) overnight at 4 °C, followed by goat anti-rat (1:1000; 112-065-003, Jackson Immuno Research Inc., Bellefonte, PA, USA) secondary antibody incubation for 2 h at room temperature. .. The signal of the secondary antibody was amplified with VECTASTAIN Elite ABC Kit (Vector Laboratories, Carlsbad, CA, USA).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Jackson Immuno messenger rna mesothelin ss1 car
    ( A ) Cytokine secretion detected at 24 hours with T cells (patients [PT], n = 9) engineered to express a <t>mesothelin-specific</t> <t>CAR</t> and stimulated with mesothelin-expressing K562 cells. ( B ) Gene set enrichment analysis of T-cell effector signaling genes in peripheral blood CD8 + T cells isolated from patients ( n = 6) compared with healthy volunteers (HV) ( n = 6). ( C ) Heat map of genes expressed by CD3 + CD8 + T cells. ( D ) Messenger <t>RNA</t> levels of genes associated with differentiation, signaling, homing, and survival detected in sorted peripheral blood T cells as indicated. GM-CSF, granulocyte-macrophage–colony-stimulating factor. ∗ P
    Messenger Rna Mesothelin Ss1 Car, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/messenger rna mesothelin ss1 car/product/Jackson Immuno
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    messenger rna mesothelin ss1 car - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    96
    Jackson Immuno biotin sp affinipure fab fragment goat anti rat igg h l
    ( A ) Cytokine secretion detected at 24 hours with T cells (patients [PT], n = 9) engineered to express a <t>mesothelin-specific</t> <t>CAR</t> and stimulated with mesothelin-expressing K562 cells. ( B ) Gene set enrichment analysis of T-cell effector signaling genes in peripheral blood CD8 + T cells isolated from patients ( n = 6) compared with healthy volunteers (HV) ( n = 6). ( C ) Heat map of genes expressed by CD3 + CD8 + T cells. ( D ) Messenger <t>RNA</t> levels of genes associated with differentiation, signaling, homing, and survival detected in sorted peripheral blood T cells as indicated. GM-CSF, granulocyte-macrophage–colony-stimulating factor. ∗ P
    Biotin Sp Affinipure Fab Fragment Goat Anti Rat Igg H L, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp affinipure fab fragment goat anti rat igg h l/product/Jackson Immuno
    Average 96 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure fab fragment goat anti rat igg h l - by Bioz Stars, 2020-04
    96/100 stars
      Buy from Supplier

    99
    Jackson Immuno biotin sp conjugated affinipure f
    ( A ) Cytokine secretion detected at 24 hours with T cells (patients [PT], n = 9) engineered to express a <t>mesothelin-specific</t> <t>CAR</t> and stimulated with mesothelin-expressing K562 cells. ( B ) Gene set enrichment analysis of T-cell effector signaling genes in peripheral blood CD8 + T cells isolated from patients ( n = 6) compared with healthy volunteers (HV) ( n = 6). ( C ) Heat map of genes expressed by CD3 + CD8 + T cells. ( D ) Messenger <t>RNA</t> levels of genes associated with differentiation, signaling, homing, and survival detected in sorted peripheral blood T cells as indicated. GM-CSF, granulocyte-macrophage–colony-stimulating factor. ∗ P
    Biotin Sp Conjugated Affinipure F, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp conjugated affinipure f/product/Jackson Immuno
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin sp conjugated affinipure f - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    86
    Jackson Immuno biotin sp conjugated affinipure goat anti rat igg
    ( A ) Cytokine secretion detected at 24 hours with T cells (patients [PT], n = 9) engineered to express a <t>mesothelin-specific</t> <t>CAR</t> and stimulated with mesothelin-expressing K562 cells. ( B ) Gene set enrichment analysis of T-cell effector signaling genes in peripheral blood CD8 + T cells isolated from patients ( n = 6) compared with healthy volunteers (HV) ( n = 6). ( C ) Heat map of genes expressed by CD3 + CD8 + T cells. ( D ) Messenger <t>RNA</t> levels of genes associated with differentiation, signaling, homing, and survival detected in sorted peripheral blood T cells as indicated. GM-CSF, granulocyte-macrophage–colony-stimulating factor. ∗ P
    Biotin Sp Conjugated Affinipure Goat Anti Rat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp conjugated affinipure goat anti rat igg/product/Jackson Immuno
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin sp conjugated affinipure goat anti rat igg - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    Image Search Results


    ( A ) Cytokine secretion detected at 24 hours with T cells (patients [PT], n = 9) engineered to express a mesothelin-specific CAR and stimulated with mesothelin-expressing K562 cells. ( B ) Gene set enrichment analysis of T-cell effector signaling genes in peripheral blood CD8 + T cells isolated from patients ( n = 6) compared with healthy volunteers (HV) ( n = 6). ( C ) Heat map of genes expressed by CD3 + CD8 + T cells. ( D ) Messenger RNA levels of genes associated with differentiation, signaling, homing, and survival detected in sorted peripheral blood T cells as indicated. GM-CSF, granulocyte-macrophage–colony-stimulating factor. ∗ P

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    Article Title: Peripheral Blood T-Cell Fitness Is Diminished in Patients With Pancreatic Carcinoma but Can Be Improved With Homeostatic Cytokines

    doi: 10.1016/j.jcmgh.2019.07.008

    Figure Lengend Snippet: ( A ) Cytokine secretion detected at 24 hours with T cells (patients [PT], n = 9) engineered to express a mesothelin-specific CAR and stimulated with mesothelin-expressing K562 cells. ( B ) Gene set enrichment analysis of T-cell effector signaling genes in peripheral blood CD8 + T cells isolated from patients ( n = 6) compared with healthy volunteers (HV) ( n = 6). ( C ) Heat map of genes expressed by CD3 + CD8 + T cells. ( D ) Messenger RNA levels of genes associated with differentiation, signaling, homing, and survival detected in sorted peripheral blood T cells as indicated. GM-CSF, granulocyte-macrophage–colony-stimulating factor. ∗ P

    Article Snippet: Mesothelin ss1 CAR expression was detected after electroporation with messenger RNA mesothelin ss1 CAR (by biotin-SP(long spacer) affiniPure goat-anti-mouse IgG; Jackson ImmunoResearch (West Grove, PA); and streptavidin-phycoerythrin (PE) as secondary antibody; BD, Franklin Lakes, NJ).

    Techniques: Expressing, Isolation