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Jackson Immuno biotin sp affinipure goat anti mouse igg
Biotin Sp Affinipure Goat Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotin sp affinipure goat anti mouse igg/product/Jackson Immuno
Average 99 stars, based on 30 article reviews
Price from $9.99 to $1999.99
biotin sp affinipure goat anti mouse igg - by Bioz Stars, 2020-03
99/100 stars

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Related Articles

Immunohistochemistry:

Article Title: Melatonin in the mammalian olfactory bulb
Article Snippet: Primary antisera used in this study were the following: rabbit anti-MT1R (1:1000 in 1% milk/TBST; Abbiotec, 250761), rabbit anti-MT1R (1:1000 in 4% milk/TBST; 1:3000 in Ni-DAB IHC; Novus, NBP1-71113), goat anti-MT2R (1:500 in 4% milk/TBST; Santa Cruz, sc-13177), rabbit anti-MT2R (1:1000 in 4% milk/TBST, 1:10,000 in Ni-DAB IHC, 1:500 in fluorescent IHC (FIHC) using streptavidin and a biotinylated secondary antibody; Novus, NLS932), mouse antityrosine hydroxylase (1:30,000 in FIHC; Chemicon, MAB318), mouse anti-glutamic acid decarboxylase (GAD)-65 (1:10,000 in FIHC; Abcam, ab26113), mouse anti-GAD67 (1:10,000 in FIHC; Chemicon, MAB5406), goat anti-calretinin (1:30,000 in FIHC; Chemicon, AB1550), goat anti-parvalbumin (1:2000 in FIHC; Swant, PVG-214), rabbit monoclonal anti-β-III-tubulin (1:2000 in 4% milk/TBST; Cell Signaling Technology, 5568), and rabbit anti-connexin43 (1:1000 in immunocytochemistry; Millipore AB1728). .. Secondary antibodies used in this study were the following: horseradish peroxidase (HRP)-conjugated goat anti-rabbit (1:10,000 in 4% milk/TBST; Bio-Rad 170-5046), HRP-conjugated goat antimouse (1:10,000 in 4% milk/TBST; Bio-Rad 170-5047), HRP-conjugated donkey anti-goat (1:40,000 in 1% milk/TBST; Jackson ImmunoResearch, 705-035-001), biotinylated horse anti-rabbit (1:1000, Vector, BA-1100), biotinylated horse anti-goat (1:1000, Vector, BA-9500), biotinylated goat anti-rabbit (1:1000, Jackson ImmunoResearch), biotinylated goat anti-mouse (1:1000, Jackson ImmunoResearch, 115-065-003), fluorescein-conjugated horse anti-mouse (1:1000, Vector, FI-2000), Cy3-conjugated goat anti-rabbit (1:1000, Jackson ImmunoResearch, 111-165-144), and Cy3-conjugated donkey anti-goat (1:1000, Jackson ImmunoResearch, 705-165-147).

Article Title: New Insights Into Marburg Virus Disease Pathogenesis in the Rhesus Macaque Model
Article Snippet: .. MARV immunohistochemistry (IHC) was performed with an antiglycoprotein (MARV glycoprotein [GP], 1:3000; rabbit polyclonal; IBT Bioservices catalog No. 0303-007) or antimatrix protein (VP40, 1:3000; mouse monoclonal; IBT Bioservices catalog No. 0203-012) primary antibody, followed by a biotinylated antirabbit secondary antibody (catalog No. 111-065-144; Jackson Immunoresearch Laboratories) or antimouse secondary antibody (catalog No. 115-065-166; Jackson Immunoresearch Laboratories) and an avidin-biotin peroxidase tertiary antibody (catalog No. PK-6100; Vector Laboratories). .. For tissues other than eye, staining was visualized with 3,3’-diaminobenzidine (brown) chromogen (catalog No. BDB2004L; Biocare Medical) and counterstained with hematoxylin.

Immunocytochemistry:

Article Title: Melatonin in the mammalian olfactory bulb
Article Snippet: Primary antisera used in this study were the following: rabbit anti-MT1R (1:1000 in 1% milk/TBST; Abbiotec, 250761), rabbit anti-MT1R (1:1000 in 4% milk/TBST; 1:3000 in Ni-DAB IHC; Novus, NBP1-71113), goat anti-MT2R (1:500 in 4% milk/TBST; Santa Cruz, sc-13177), rabbit anti-MT2R (1:1000 in 4% milk/TBST, 1:10,000 in Ni-DAB IHC, 1:500 in fluorescent IHC (FIHC) using streptavidin and a biotinylated secondary antibody; Novus, NLS932), mouse antityrosine hydroxylase (1:30,000 in FIHC; Chemicon, MAB318), mouse anti-glutamic acid decarboxylase (GAD)-65 (1:10,000 in FIHC; Abcam, ab26113), mouse anti-GAD67 (1:10,000 in FIHC; Chemicon, MAB5406), goat anti-calretinin (1:30,000 in FIHC; Chemicon, AB1550), goat anti-parvalbumin (1:2000 in FIHC; Swant, PVG-214), rabbit monoclonal anti-β-III-tubulin (1:2000 in 4% milk/TBST; Cell Signaling Technology, 5568), and rabbit anti-connexin43 (1:1000 in immunocytochemistry; Millipore AB1728). .. Secondary antibodies used in this study were the following: horseradish peroxidase (HRP)-conjugated goat anti-rabbit (1:10,000 in 4% milk/TBST; Bio-Rad 170-5046), HRP-conjugated goat antimouse (1:10,000 in 4% milk/TBST; Bio-Rad 170-5047), HRP-conjugated donkey anti-goat (1:40,000 in 1% milk/TBST; Jackson ImmunoResearch, 705-035-001), biotinylated horse anti-rabbit (1:1000, Vector, BA-1100), biotinylated horse anti-goat (1:1000, Vector, BA-9500), biotinylated goat anti-rabbit (1:1000, Jackson ImmunoResearch), biotinylated goat anti-mouse (1:1000, Jackson ImmunoResearch, 115-065-003), fluorescein-conjugated horse anti-mouse (1:1000, Vector, FI-2000), Cy3-conjugated goat anti-rabbit (1:1000, Jackson ImmunoResearch, 111-165-144), and Cy3-conjugated donkey anti-goat (1:1000, Jackson ImmunoResearch, 705-165-147).

Flow Cytometry:

Article Title: Haploidentical CD19/CD22 bispecific CAR-T cells induced MRD-negative remission in a patient with relapsed and refractory adult B-ALL after haploidentical hematopoietic stem cell transplantation
Article Snippet: .. Detection of haplo-TanCAR-T 19/22 cells Flow cytometry was used for the determination of the TanCAR-19/22 transfection efficiency and quantification of the haplo-TanCAR-T 19/22 cells in clinical specimens using a Biotin-SP-AffiniPure Goat Anti-Mouse IgG, F (ab') 2 Fragment Specific (Jackson ImmunoResearch, USA) and PE Streptavidin antibody (BD Biosciences, USA). .. Haplo-TanCAR-T 19/22 cells in clinical specimens also were measured by qPCR as described [ ].

Avidin-Biotin Assay:

Article Title: New Insights Into Marburg Virus Disease Pathogenesis in the Rhesus Macaque Model
Article Snippet: .. MARV immunohistochemistry (IHC) was performed with an antiglycoprotein (MARV glycoprotein [GP], 1:3000; rabbit polyclonal; IBT Bioservices catalog No. 0303-007) or antimatrix protein (VP40, 1:3000; mouse monoclonal; IBT Bioservices catalog No. 0203-012) primary antibody, followed by a biotinylated antirabbit secondary antibody (catalog No. 111-065-144; Jackson Immunoresearch Laboratories) or antimouse secondary antibody (catalog No. 115-065-166; Jackson Immunoresearch Laboratories) and an avidin-biotin peroxidase tertiary antibody (catalog No. PK-6100; Vector Laboratories). .. For tissues other than eye, staining was visualized with 3,3’-diaminobenzidine (brown) chromogen (catalog No. BDB2004L; Biocare Medical) and counterstained with hematoxylin.

Article Title: Neuronal network dysfunction precedes storage and neurodegeneration in a lysosomal storage disorder
Article Snippet: To detect the primary antibody, sections were incubated with a biotinylated goat antimouse secondary antibody (Jackson ImmunoResearch 115-065-062, 1:500) at room temperature for 30 minutes. .. After PBS washes, sections were incubated in avidin-biotin complex (Vectastain, Vector Laboratories) at room temperature for 1 hour.

Microscopy:

Article Title: Neuronal network dysfunction precedes storage and neurodegeneration in a lysosomal storage disorder
Article Snippet: For measurement of autofluorescence, unstained immunofluorescence images of the DG region were acquired using a Leica DMR microscope coupled with a CoolSNAP camera (Leica Microsystems). .. To detect the primary antibody, sections were incubated with a biotinylated goat antimouse secondary antibody (Jackson ImmunoResearch 115-065-062, 1:500) at room temperature for 30 minutes.

Cytometry:

Article Title: Haploidentical CD19/CD22 bispecific CAR-T cells induced MRD-negative remission in a patient with relapsed and refractory adult B-ALL after haploidentical hematopoietic stem cell transplantation
Article Snippet: .. Detection of haplo-TanCAR-T 19/22 cells Flow cytometry was used for the determination of the TanCAR-19/22 transfection efficiency and quantification of the haplo-TanCAR-T 19/22 cells in clinical specimens using a Biotin-SP-AffiniPure Goat Anti-Mouse IgG, F (ab') 2 Fragment Specific (Jackson ImmunoResearch, USA) and PE Streptavidin antibody (BD Biosciences, USA). .. Haplo-TanCAR-T 19/22 cells in clinical specimens also were measured by qPCR as described [ ].

Real-time Polymerase Chain Reaction:

Article Title: Haploidentical CD19/CD22 bispecific CAR-T cells induced MRD-negative remission in a patient with relapsed and refractory adult B-ALL after haploidentical hematopoietic stem cell transplantation
Article Snippet: Detection of haplo-TanCAR-T 19/22 cells Flow cytometry was used for the determination of the TanCAR-19/22 transfection efficiency and quantification of the haplo-TanCAR-T 19/22 cells in clinical specimens using a Biotin-SP-AffiniPure Goat Anti-Mouse IgG, F (ab') 2 Fragment Specific (Jackson ImmunoResearch, USA) and PE Streptavidin antibody (BD Biosciences, USA). .. Haplo-TanCAR-T 19/22 cells in clinical specimens also were measured by qPCR as described [ ].

Immunoprecipitation:

Article Title: Vaccinia virus egress mediated by virus protein A36 is reliant on the F12 protein
Article Snippet: Immunoprecipitated proteins were eluted by boiling in Laemmli loading buffer and analysed by SDS-PAGE and immunoblotting. .. Detection of the A36 protein, which has a similar molecular mass to the antibody heavy chain, was achieved by using a Biotin-SP AffiniPure goat anti-mouse IgG, light chain specific (115-065-174; Jackson ImmunoResearch).

Article Title: Vaccinia virus proteins A36 and F12/ E2 show strong preferences for different kinesin light chain isoforms. Vaccinia virus proteins A36 and F12/E2 show strong preferences for different kinesin light chain isoforms
Article Snippet: Immunoprecipitated proteins were eluted by boiling in Laemmli SDS‐PAGE loading buffer prior to analysis by SDS‐PAGE and immunoblotting. .. A33 and A34, which have overlapping molecular masses to the antibody light chain, were detected using a biotin‐SP AffiniPure goat anti‐mouse IgG, Fcγ fragment specific (Jackson ImmunoResearch, 115‐065‐071).

Incubation:

Article Title: Vaccinia virus egress mediated by virus protein A36 is reliant on the F12 protein
Article Snippet: Immunoprecipitations were incubated with rotation overnight and then washed four times with IP wash buffer. .. Detection of the A36 protein, which has a similar molecular mass to the antibody heavy chain, was achieved by using a Biotin-SP AffiniPure goat anti-mouse IgG, light chain specific (115-065-174; Jackson ImmunoResearch).

Article Title: Neuronal network dysfunction precedes storage and neurodegeneration in a lysosomal storage disorder
Article Snippet: .. To detect the primary antibody, sections were incubated with a biotinylated goat antimouse secondary antibody (Jackson ImmunoResearch 115-065-062, 1:500) at room temperature for 30 minutes. .. After PBS washes, sections were incubated in avidin-biotin complex (Vectastain, Vector Laboratories) at room temperature for 1 hour.

Article Title: Vaccinia virus proteins A36 and F12/ E2 show strong preferences for different kinesin light chain isoforms. Vaccinia virus proteins A36 and F12/E2 show strong preferences for different kinesin light chain isoforms
Article Snippet: Immunoprecipitations were incubated for 4 h or overnight while rotating and then washed 4 times with IP wash buffer. .. A33 and A34, which have overlapping molecular masses to the antibody light chain, were detected using a biotin‐SP AffiniPure goat anti‐mouse IgG, Fcγ fragment specific (Jackson ImmunoResearch, 115‐065‐071).

Transfection:

Article Title: Haploidentical CD19/CD22 bispecific CAR-T cells induced MRD-negative remission in a patient with relapsed and refractory adult B-ALL after haploidentical hematopoietic stem cell transplantation
Article Snippet: .. Detection of haplo-TanCAR-T 19/22 cells Flow cytometry was used for the determination of the TanCAR-19/22 transfection efficiency and quantification of the haplo-TanCAR-T 19/22 cells in clinical specimens using a Biotin-SP-AffiniPure Goat Anti-Mouse IgG, F (ab') 2 Fragment Specific (Jackson ImmunoResearch, USA) and PE Streptavidin antibody (BD Biosciences, USA). .. Haplo-TanCAR-T 19/22 cells in clinical specimens also were measured by qPCR as described [ ].

Plasmid Preparation:

Article Title: Melatonin in the mammalian olfactory bulb
Article Snippet: .. Secondary antibodies used in this study were the following: horseradish peroxidase (HRP)-conjugated goat anti-rabbit (1:10,000 in 4% milk/TBST; Bio-Rad 170-5046), HRP-conjugated goat antimouse (1:10,000 in 4% milk/TBST; Bio-Rad 170-5047), HRP-conjugated donkey anti-goat (1:40,000 in 1% milk/TBST; Jackson ImmunoResearch, 705-035-001), biotinylated horse anti-rabbit (1:1000, Vector, BA-1100), biotinylated horse anti-goat (1:1000, Vector, BA-9500), biotinylated goat anti-rabbit (1:1000, Jackson ImmunoResearch), biotinylated goat anti-mouse (1:1000, Jackson ImmunoResearch, 115-065-003), fluorescein-conjugated horse anti-mouse (1:1000, Vector, FI-2000), Cy3-conjugated goat anti-rabbit (1:1000, Jackson ImmunoResearch, 111-165-144), and Cy3-conjugated donkey anti-goat (1:1000, Jackson ImmunoResearch, 705-165-147). .. Both Cy3- (Jackson ImmunoResearch, 016-160-084) and Alexa488-conjugated (Life Technologies, S32354) streptavidin were used at 1:1000 in this study, diluted in 0.4% Triton X-100/PBS for FIHC.

Article Title: Neuronal network dysfunction precedes storage and neurodegeneration in a lysosomal storage disorder
Article Snippet: To detect the primary antibody, sections were incubated with a biotinylated goat antimouse secondary antibody (Jackson ImmunoResearch 115-065-062, 1:500) at room temperature for 30 minutes. .. After PBS washes, sections were incubated in avidin-biotin complex (Vectastain, Vector Laboratories) at room temperature for 1 hour.

Staining:

Article Title: New Insights Into Marburg Virus Disease Pathogenesis in the Rhesus Macaque Model
Article Snippet: MARV immunohistochemistry (IHC) was performed with an antiglycoprotein (MARV glycoprotein [GP], 1:3000; rabbit polyclonal; IBT Bioservices catalog No. 0303-007) or antimatrix protein (VP40, 1:3000; mouse monoclonal; IBT Bioservices catalog No. 0203-012) primary antibody, followed by a biotinylated antirabbit secondary antibody (catalog No. 111-065-144; Jackson Immunoresearch Laboratories) or antimouse secondary antibody (catalog No. 115-065-166; Jackson Immunoresearch Laboratories) and an avidin-biotin peroxidase tertiary antibody (catalog No. PK-6100; Vector Laboratories). .. For tissues other than eye, staining was visualized with 3,3’-diaminobenzidine (brown) chromogen (catalog No. BDB2004L; Biocare Medical) and counterstained with hematoxylin.

Article Title: Neuronal network dysfunction precedes storage and neurodegeneration in a lysosomal storage disorder
Article Snippet: For NeuN staining, sections were washed with PBS, permeabilized with 0.1% Triton X-100 in PBS for 7 minutes, incubated in 3% H2 O2 /10% methanol in PBS for 5 minutes, washed again with PBS 3 times, and blocked in 10% normal goat serum in PBS, all at room temperature. .. To detect the primary antibody, sections were incubated with a biotinylated goat antimouse secondary antibody (Jackson ImmunoResearch 115-065-062, 1:500) at room temperature for 30 minutes.

Centrifugation:

Article Title: Vaccinia virus egress mediated by virus protein A36 is reliant on the F12 protein
Article Snippet: Lysates were clarified by centrifugation (15 000 g , 15 min, 4 °C) and Flag-tagged proteins were immunoprecipitated using anti-FLAG M2 affinity gel (Sigma). .. Detection of the A36 protein, which has a similar molecular mass to the antibody heavy chain, was achieved by using a Biotin-SP AffiniPure goat anti-mouse IgG, light chain specific (115-065-174; Jackson ImmunoResearch).

Immunofluorescence:

Article Title: Neuronal network dysfunction precedes storage and neurodegeneration in a lysosomal storage disorder
Article Snippet: For measurement of autofluorescence, unstained immunofluorescence images of the DG region were acquired using a Leica DMR microscope coupled with a CoolSNAP camera (Leica Microsystems). .. To detect the primary antibody, sections were incubated with a biotinylated goat antimouse secondary antibody (Jackson ImmunoResearch 115-065-062, 1:500) at room temperature for 30 minutes.

SDS Page:

Article Title: Vaccinia virus egress mediated by virus protein A36 is reliant on the F12 protein
Article Snippet: Proteins separated by SDS-PAGE and transferred onto Hybond ECL nitrocellulose membrane (GE Healthcare) were probed with rabbit polyclonal αFLAG (F7425; Sigma-Aldrich, 1 : 5 000), mouse monoclonal αA36 [ ] and mouse monoclonal AB1.1 specific for the VACV protein D8 [ ]. .. Detection of the A36 protein, which has a similar molecular mass to the antibody heavy chain, was achieved by using a Biotin-SP AffiniPure goat anti-mouse IgG, light chain specific (115-065-174; Jackson ImmunoResearch).

Article Title: Vaccinia virus proteins A36 and F12/ E2 show strong preferences for different kinesin light chain isoforms. Vaccinia virus proteins A36 and F12/E2 show strong preferences for different kinesin light chain isoforms
Article Snippet: Proteins separated by SDS‐PAGE and transferred onto Hybond ECL nitrocellulose membrane (GE Healthcare) were probed with the following commercial antibodies; rabbit polyclonal α‐FLAG (Sigma‐Aldrich, F7425, 1:5000), rabbit polyclonal α‐HA (Sigma‐Aldrich, H6908, 1:1500), rat monoclonal α‐HA (Chromotek, 7C9, 1:1000), mouse monoclonal α‐HA (BioLegend, HA.11, 1:1000), rabbit polyclonal α‐14‐3‐3 (Santa Cruz, sc‐629, 1:1000), rabbit monoclonal α‐KIF5B (Abcam, ab167429, 1:1000), the following antibodies specific for VACV proteins; mouse monoclonal α‐A36 (1:1000), rat monoclonal α‐F13 (1:1000), rat monoclonal α‐B5 (1:100), mouse monoclonal α‐A33 (1:5), mouse monoclonal α‐A34, and mouse monoclonal AB1.1 specific for the VACV protein D8. .. A33 and A34, which have overlapping molecular masses to the antibody light chain, were detected using a biotin‐SP AffiniPure goat anti‐mouse IgG, Fcγ fragment specific (Jackson ImmunoResearch, 115‐065‐071).

Software:

Article Title: Vaccinia virus proteins A36 and F12/ E2 show strong preferences for different kinesin light chain isoforms. Vaccinia virus proteins A36 and F12/E2 show strong preferences for different kinesin light chain isoforms
Article Snippet: A33 and A34, which have overlapping molecular masses to the antibody light chain, were detected using a biotin‐SP AffiniPure goat anti‐mouse IgG, Fcγ fragment specific (Jackson ImmunoResearch, 115‐065‐071). .. For quantitative analysis of relative protein levels, band intensities were measured using the LI‐COR Odyssey scanner software with localized background normalization.

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    Jackson Immuno biotin sp conjugated affinipure goat anti mouse
    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated <t>AffiniPure</t> goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p
    Biotin Sp Conjugated Affinipure Goat Anti Mouse, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 89/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp conjugated affinipure goat anti mouse/product/Jackson Immuno
    Average 89 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    biotin sp conjugated affinipure goat anti mouse - by Bioz Stars, 2020-03
    89/100 stars
      Buy from Supplier

    99
    Jackson Immuno biotin sp affinipure goat anti mouse igg f ab 2 fragment specific
    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated <t>AffiniPure</t> goat anti-mouse <t>IgG,F(ab′)2</t> fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p
    Biotin Sp Affinipure Goat Anti Mouse Igg F Ab 2 Fragment Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp affinipure goat anti mouse igg f ab 2 fragment specific/product/Jackson Immuno
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure goat anti mouse igg f ab 2 fragment specific - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    99
    Jackson Immuno igm
    DRibbles derived from tumor cells induce specific antibody production in vivo. (A and B) Serum was collected from C57/BL6 mice at day 7 after first intravenous injection of PBS or Hep1-6 derived DRibbles (DRs). The total <t>IgM</t> (A) and <t>IgG</t> (B) in serum was measured by ELISA. Values are the mean ± SEM derived from three mice per group (n = 3). (C and D) Hep1-6 or B16F10 cells were incubated for 1 hour with serum derived from PBS or Hep1-6 DRibbles injected mice respectively. After washing, Hep1-6 or B16F10 cells were incubated with FITC-labeled anti-mouse IgM antibody (C) or FITC-labeled anti-mouse IgG antibody (D). After washing, the cells were analyzed by flow cytometry. (E and F) HepI-6 or B16F10 cells were treated as C and D, and then the cells were stained with DAPI and analyzed by fluorescence microscope. Green fluorescence represents FITC-labeled anti-mouse IgM antibody (E) or FITC-labeled anti-mouse IgG antibody (F). Blue fluorescence represents the cell nucleus. A representative of three independent experiments was showed.
    Igm, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igm/product/Jackson Immuno
    Average 99 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    igm - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p

    Journal: PLoS ONE

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis

    doi: 10.1371/journal.pone.0109352

    Figure Lengend Snippet: Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p

    Article Snippet: After washing, the cells were stained with biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072), followed by PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) and CD56-FITC.

    Techniques: Flow Cytometry, Cytometry, Cell Culture, Staining, Irradiation

    Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.

    Journal: PLoS ONE

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis

    doi: 10.1371/journal.pone.0109352

    Figure Lengend Snippet: Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.

    Article Snippet: After washing, the cells were stained with biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072), followed by PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) and CD56-FITC.

    Techniques: Immunofluorescence, Staining, Cell Culture

    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p

    Journal: PLoS ONE

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis

    doi: 10.1371/journal.pone.0109352

    Figure Lengend Snippet: Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p

    Article Snippet: Biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072) and PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) were used for labeling the cells.

    Techniques: Flow Cytometry, Cytometry, Cell Culture, Staining, Irradiation

    Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.

    Journal: PLoS ONE

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis

    doi: 10.1371/journal.pone.0109352

    Figure Lengend Snippet: Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.

    Article Snippet: Biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072) and PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) were used for labeling the cells.

    Techniques: Immunofluorescence, Staining, Cell Culture

    DRibbles derived from tumor cells induce specific antibody production in vivo. (A and B) Serum was collected from C57/BL6 mice at day 7 after first intravenous injection of PBS or Hep1-6 derived DRibbles (DRs). The total IgM (A) and IgG (B) in serum was measured by ELISA. Values are the mean ± SEM derived from three mice per group (n = 3). (C and D) Hep1-6 or B16F10 cells were incubated for 1 hour with serum derived from PBS or Hep1-6 DRibbles injected mice respectively. After washing, Hep1-6 or B16F10 cells were incubated with FITC-labeled anti-mouse IgM antibody (C) or FITC-labeled anti-mouse IgG antibody (D). After washing, the cells were analyzed by flow cytometry. (E and F) HepI-6 or B16F10 cells were treated as C and D, and then the cells were stained with DAPI and analyzed by fluorescence microscope. Green fluorescence represents FITC-labeled anti-mouse IgM antibody (E) or FITC-labeled anti-mouse IgG antibody (F). Blue fluorescence represents the cell nucleus. A representative of three independent experiments was showed.

    Journal: PLoS ONE

    Article Title: Tumor-Derived Autophagosomes (DRibbles) Induce B Cell Activation in a TLR2-MyD88 Dependent Manner

    doi: 10.1371/journal.pone.0053564

    Figure Lengend Snippet: DRibbles derived from tumor cells induce specific antibody production in vivo. (A and B) Serum was collected from C57/BL6 mice at day 7 after first intravenous injection of PBS or Hep1-6 derived DRibbles (DRs). The total IgM (A) and IgG (B) in serum was measured by ELISA. Values are the mean ± SEM derived from three mice per group (n = 3). (C and D) Hep1-6 or B16F10 cells were incubated for 1 hour with serum derived from PBS or Hep1-6 DRibbles injected mice respectively. After washing, Hep1-6 or B16F10 cells were incubated with FITC-labeled anti-mouse IgM antibody (C) or FITC-labeled anti-mouse IgG antibody (D). After washing, the cells were analyzed by flow cytometry. (E and F) HepI-6 or B16F10 cells were treated as C and D, and then the cells were stained with DAPI and analyzed by fluorescence microscope. Green fluorescence represents FITC-labeled anti-mouse IgM antibody (E) or FITC-labeled anti-mouse IgG antibody (F). Blue fluorescence represents the cell nucleus. A representative of three independent experiments was showed.

    Article Snippet: Briefly, The plates were coated with either goat anti-mouse IgG or IgM (Jackson Immuno Research Laboratories) at 1∶10000 overnight at 4°C and washed with ELISA washing buffer (PBS plus 0.5% Tween-20).

    Techniques: Derivative Assay, In Vivo, Mouse Assay, Injection, Enzyme-linked Immunosorbent Assay, Incubation, Labeling, Flow Cytometry, Cytometry, Staining, Fluorescence, Microscopy