biotin sp affinipure goat anti mouse igg igm  (Jackson Immuno)

 
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  • 99
    Name:
    Biotin SP AffiniPure Goat Anti Mouse IgG IgM
    Description:
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with both mouse IgG and IgM It also reacts with the light chains of other mouse immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    Catalog Number:
    115-065-044
    Price:
    134
    Purity:
    The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
    Conjugate:
    Biotin SP long spacer
    Size:
    ml
    Category:
    Secondary Antibody
    Source:
    Goat
    Quantity:
    2 0
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    Structured Review

    Jackson Immuno biotin sp affinipure goat anti mouse igg igm
    Biotin SP AffiniPure Goat Anti Mouse IgG IgM
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with both mouse IgG and IgM It also reacts with the light chains of other mouse immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    https://www.bioz.com/result/biotin sp affinipure goat anti mouse igg igm/product/Jackson Immuno
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure goat anti mouse igg igm - by Bioz Stars, 2020-04
    99/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Differential Expression of IGF Components and Insulin Receptor Isoforms in Human Seminoma Versus Normal Testicular Tissue 1
    Article Snippet: Immunoreactive sites were detected using incubation with a 1:1000 dilution of biotin SP-conjugated goat antimouse IgG/IgM (Jackson Immuno Research, West Grove, PA) for 30 minutes at room temperature (21°C) and subsequent incubation with streptavidin-conjugated alkaline phosphatase (Jackson Immuno Research) diluted to 1:250 at room temperature (21°C) for 30 minutes. .. Stained slides were examined to identify the cellular localization of IGF-2 immunoreactivity.

    Incubation:

    Article Title: Differential Expression of IGF Components and Insulin Receptor Isoforms in Human Seminoma Versus Normal Testicular Tissue 1
    Article Snippet: .. Immunoreactive sites were detected using incubation with a 1:1000 dilution of biotin SP-conjugated goat antimouse IgG/IgM (Jackson Immuno Research, West Grove, PA) for 30 minutes at room temperature (21°C) and subsequent incubation with streptavidin-conjugated alkaline phosphatase (Jackson Immuno Research) diluted to 1:250 at room temperature (21°C) for 30 minutes. .. Immunoreactive sites were developed using Fuchsine substrate chromogen (Dako, Glostrup, Denmark).

    Article Title: Reduced Expression of GDF-15 Is Associated With Atrophic Inflammatory Lesions of the Prostate
    Article Snippet: .. Biotinylated secondary antibodies from Jackson ImmunoResearch raised against mouse (goat anti-mouse; cat# 115-065-068; 1:400), rabbit (goat anti-rabbit; cat# 111-065-144; 1:400) and goat (rabbit anti-goat; cat# 305-065-045; 1:400) were all sourced from Jackson ImmunoRe-search, diluted in TBST and incubated for 30 min. .. Secondary antibodies were detected with streptavidin–horseradish peroxidase (Vector Laboratories; cat# SA-5004; 1:200) diluted in TBST and incubated for 30 min. Visualization of antibody complexes was accomplished with 3,3′ diaminobenzidine (Biocare; cat# DBC859L10) for 5 min. All slides were rinsed thoroughly in deionized water, counterstained in Mayer’s hematoxylin for 2 min, nuclei blued in 1% ammonium hydroxide (aq.), dehydrated in graded alcohols, cleared in xylene and cover glass was mounted with synthetic resin.

    Immunohistochemistry:

    Article Title: Differential Expression of IGF Components and Insulin Receptor Isoforms in Human Seminoma Versus Normal Testicular Tissue 1
    Article Snippet: Paragraph title: Immunohistochemistry ... Immunoreactive sites were detected using incubation with a 1:1000 dilution of biotin SP-conjugated goat antimouse IgG/IgM (Jackson Immuno Research, West Grove, PA) for 30 minutes at room temperature (21°C) and subsequent incubation with streptavidin-conjugated alkaline phosphatase (Jackson Immuno Research) diluted to 1:250 at room temperature (21°C) for 30 minutes.

    Article Title: Reduced Expression of GDF-15 Is Associated With Atrophic Inflammatory Lesions of the Prostate
    Article Snippet: Paragraph title: Immunohistochemistry ... Biotinylated secondary antibodies from Jackson ImmunoResearch raised against mouse (goat anti-mouse; cat# 115-065-068; 1:400), rabbit (goat anti-rabbit; cat# 111-065-144; 1:400) and goat (rabbit anti-goat; cat# 305-065-045; 1:400) were all sourced from Jackson ImmunoRe-search, diluted in TBST and incubated for 30 min.

    Plasmid Preparation:

    Article Title: Reduced Expression of GDF-15 Is Associated With Atrophic Inflammatory Lesions of the Prostate
    Article Snippet: Biotinylated secondary antibodies from Jackson ImmunoResearch raised against mouse (goat anti-mouse; cat# 115-065-068; 1:400), rabbit (goat anti-rabbit; cat# 111-065-144; 1:400) and goat (rabbit anti-goat; cat# 305-065-045; 1:400) were all sourced from Jackson ImmunoRe-search, diluted in TBST and incubated for 30 min. .. Secondary antibodies were detected with streptavidin–horseradish peroxidase (Vector Laboratories; cat# SA-5004; 1:200) diluted in TBST and incubated for 30 min. Visualization of antibody complexes was accomplished with 3,3′ diaminobenzidine (Biocare; cat# DBC859L10) for 5 min. All slides were rinsed thoroughly in deionized water, counterstained in Mayer’s hematoxylin for 2 min, nuclei blued in 1% ammonium hydroxide (aq.), dehydrated in graded alcohols, cleared in xylene and cover glass was mounted with synthetic resin.

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  • 99
    Jackson Immuno igm
    DRibbles derived from tumor cells induce specific antibody production in vivo. (A and B) Serum was collected from C57/BL6 mice at day 7 after first intravenous injection of PBS or Hep1-6 derived DRibbles (DRs). The total <t>IgM</t> (A) and <t>IgG</t> (B) in serum was measured by ELISA. Values are the mean ± SEM derived from three mice per group (n = 3). (C and D) Hep1-6 or B16F10 cells were incubated for 1 hour with serum derived from PBS or Hep1-6 DRibbles injected mice respectively. After washing, Hep1-6 or B16F10 cells were incubated with FITC-labeled anti-mouse IgM antibody (C) or FITC-labeled anti-mouse IgG antibody (D). After washing, the cells were analyzed by flow cytometry. (E and F) HepI-6 or B16F10 cells were treated as C and D, and then the cells were stained with DAPI and analyzed by fluorescence microscope. Green fluorescence represents FITC-labeled anti-mouse IgM antibody (E) or FITC-labeled anti-mouse IgG antibody (F). Blue fluorescence represents the cell nucleus. A representative of three independent experiments was showed.
    Igm, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igm/product/Jackson Immuno
    Average 99 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    igm - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    DRibbles derived from tumor cells induce specific antibody production in vivo. (A and B) Serum was collected from C57/BL6 mice at day 7 after first intravenous injection of PBS or Hep1-6 derived DRibbles (DRs). The total IgM (A) and IgG (B) in serum was measured by ELISA. Values are the mean ± SEM derived from three mice per group (n = 3). (C and D) Hep1-6 or B16F10 cells were incubated for 1 hour with serum derived from PBS or Hep1-6 DRibbles injected mice respectively. After washing, Hep1-6 or B16F10 cells were incubated with FITC-labeled anti-mouse IgM antibody (C) or FITC-labeled anti-mouse IgG antibody (D). After washing, the cells were analyzed by flow cytometry. (E and F) HepI-6 or B16F10 cells were treated as C and D, and then the cells were stained with DAPI and analyzed by fluorescence microscope. Green fluorescence represents FITC-labeled anti-mouse IgM antibody (E) or FITC-labeled anti-mouse IgG antibody (F). Blue fluorescence represents the cell nucleus. A representative of three independent experiments was showed.

    Journal: PLoS ONE

    Article Title: Tumor-Derived Autophagosomes (DRibbles) Induce B Cell Activation in a TLR2-MyD88 Dependent Manner

    doi: 10.1371/journal.pone.0053564

    Figure Lengend Snippet: DRibbles derived from tumor cells induce specific antibody production in vivo. (A and B) Serum was collected from C57/BL6 mice at day 7 after first intravenous injection of PBS or Hep1-6 derived DRibbles (DRs). The total IgM (A) and IgG (B) in serum was measured by ELISA. Values are the mean ± SEM derived from three mice per group (n = 3). (C and D) Hep1-6 or B16F10 cells were incubated for 1 hour with serum derived from PBS or Hep1-6 DRibbles injected mice respectively. After washing, Hep1-6 or B16F10 cells were incubated with FITC-labeled anti-mouse IgM antibody (C) or FITC-labeled anti-mouse IgG antibody (D). After washing, the cells were analyzed by flow cytometry. (E and F) HepI-6 or B16F10 cells were treated as C and D, and then the cells were stained with DAPI and analyzed by fluorescence microscope. Green fluorescence represents FITC-labeled anti-mouse IgM antibody (E) or FITC-labeled anti-mouse IgG antibody (F). Blue fluorescence represents the cell nucleus. A representative of three independent experiments was showed.

    Article Snippet: Briefly, The plates were coated with either goat anti-mouse IgG or IgM (Jackson Immuno Research Laboratories) at 1∶10000 overnight at 4°C and washed with ELISA washing buffer (PBS plus 0.5% Tween-20).

    Techniques: Derivative Assay, In Vivo, Mouse Assay, Injection, Enzyme-linked Immunosorbent Assay, Incubation, Labeling, Flow Cytometry, Cytometry, Staining, Fluorescence, Microscopy