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Jackson Immuno biotin sp affinipure goat anti human igg
Biotin Sp Affinipure Goat Anti Human Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotin sp affinipure goat anti human igg/product/Jackson Immuno
Average 96 stars, based on 1 article reviews
Price from $9.99 to $1999.99
biotin sp affinipure goat anti human igg - by Bioz Stars, 2020-03
96/100 stars

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Related Articles

Transferring:

Article Title: Vaccine-draining lymph nodes of cancer patients for generating anti-cancer antibodies
Article Snippet: Briefly, the MNCs from the lymph node were stimulated for 3 days with R-848 (Mabtech, Inc., Cincinnati, OH) and IL-2 (PeproTech, Rocky Hill, NJ), before transferring them to Multiscreen IP-PVDF filter microplates (Millipore) coated with affinipure goat anti-Human IgG Fcγ Fragment Specific (Jackson Immuno Research, West Grove PA) or vaccine peptides (2 μg total, individual or a mixture of all the six), diluted in PBS, for determining the total number of IgG-secreting B cells and anti-vaccine peptide antibody-secreting B cells, respectively. .. The cells were rinsed off the filter plate and antibody spots representing the secretion of antibody were detected with biotin-SP-affiniPure goat anti-human IgG, Fcγ (Jackson Immuno Research) and streptavidin–alkaline phosphatase conjugate (Sigma, St. Louis, MO) and visualized with SIGMAFAST 5-Bromo-4-chloro-3-indolyl phosphate/Nitro blue tetrazolium (Sigma) for 3–5 min until spots developed.

Enzyme-linked Immunospot:

Article Title: Vaccine-draining lymph nodes of cancer patients for generating anti-cancer antibodies
Article Snippet: Paragraph title: B-cell ELISPOT ... The cells were rinsed off the filter plate and antibody spots representing the secretion of antibody were detected with biotin-SP-affiniPure goat anti-human IgG, Fcγ (Jackson Immuno Research) and streptavidin–alkaline phosphatase conjugate (Sigma, St. Louis, MO) and visualized with SIGMAFAST 5-Bromo-4-chloro-3-indolyl phosphate/Nitro blue tetrazolium (Sigma) for 3–5 min until spots developed.

Incubation:

Article Title: Vaccine-draining lymph nodes of cancer patients for generating anti-cancer antibodies
Article Snippet: Following stimulation, MNCs were allowed to settle to the bottom of the coated filter plate wells as a monolayer and incubated overnight. .. The cells were rinsed off the filter plate and antibody spots representing the secretion of antibody were detected with biotin-SP-affiniPure goat anti-human IgG, Fcγ (Jackson Immuno Research) and streptavidin–alkaline phosphatase conjugate (Sigma, St. Louis, MO) and visualized with SIGMAFAST 5-Bromo-4-chloro-3-indolyl phosphate/Nitro blue tetrazolium (Sigma) for 3–5 min until spots developed.

Imaging:

Article Title: Vaccine-draining lymph nodes of cancer patients for generating anti-cancer antibodies
Article Snippet: The cells were rinsed off the filter plate and antibody spots representing the secretion of antibody were detected with biotin-SP-affiniPure goat anti-human IgG, Fcγ (Jackson Immuno Research) and streptavidin–alkaline phosphatase conjugate (Sigma, St. Louis, MO) and visualized with SIGMAFAST 5-Bromo-4-chloro-3-indolyl phosphate/Nitro blue tetrazolium (Sigma) for 3–5 min until spots developed. .. Fully dried membranes were imaged using the DigiDoc-It Imaging System (UVP, LLC, Upland, CA).

Sequencing:

Article Title: Vaccine-draining lymph nodes of cancer patients for generating anti-cancer antibodies
Article Snippet: The irrelevant synthetic peptide used had the following sequence NH2 -RVQECKYLYYDNDYLCKDDG-OH. .. The cells were rinsed off the filter plate and antibody spots representing the secretion of antibody were detected with biotin-SP-affiniPure goat anti-human IgG, Fcγ (Jackson Immuno Research) and streptavidin–alkaline phosphatase conjugate (Sigma, St. Louis, MO) and visualized with SIGMAFAST 5-Bromo-4-chloro-3-indolyl phosphate/Nitro blue tetrazolium (Sigma) for 3–5 min until spots developed.

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  • 99
    Jackson Immuno igm
    Plasma cells, B cells and macrophages in cardiac allografts with CAV (A) Left panel: Representative image of a tissue section from a CAV patient stained for CD20 + B cells (pink) and CD138 + plasma cells (brown). Middle and right panels: Immunofluorescence staining of consecutive formalin-fixed paraffin-embedded (FFPE) tissue sections from a representative CAV sample showing plasma cell–rich infiltrates adjacent to the coronary artery of a heart explant with CAV. CD138 + plasma cells (red) co-stained for secreted <t>IgM</t> or <t>IgG</t> (blue). Nuclei are shown in gray. (B) Representative staining of cardiac explant sections with CAV showing CD68 + macrophages (brown) and CD20 + B cells (pink, left panel) or CD138 + plasma cells (pink, middle panel). Right panel: Venn diagram representation of the type of infiltrates observed in the 56 CAV explants: B cell; plasma cell; and macrophage. The number of specimens with evidence of infiltrate is indicated for each cell type. One unique sample had no infiltration. (C) Left and middle left panel: Representative immunofluorescence staining of consecutive FFPE tissue sections of a cardiac explant with CAV for M2 macrophage marker CD163 (left) or M1 marker iNOS (middle left) in combination with CD68. Middle right panel: Immunofluorescence staining of an FFPE tissue section of a cardiac explant with CAV for M2 macrophage markers CD206 and CD163. Right panel: Representative immunofluorescence staining of an FFPE tissue section of a cardiac explant with CAV for IL-10 and M2 macrophage marker CD206.
    Igm, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igm/product/Jackson Immuno
    Average 99 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    igm - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    99
    Jackson Immuno biotin sp affinipure goat anti human igg fcγ fragment specific
    GMR biosensor autoantigen microarrays. ( a ) Optical images of a GMR biosensor chip and a cartridge with a reaction well (left). The sensor chip measures 10 × 12 mm and consists of an array of 8 × 10 sensors (total 80 sensors). Each sensor size is 100 × 100 μm (right). ( b ) A schematic of assaying antibody reactivity to autoantigens (not to scale). (1) Autoantigens were printed on the surface of the chip’s sensors. (2) The sample was added to the reaction well, allowing antibodies to bind to their corresponding antigens. (3) After washing, species-specific, <t>biotinylated</t> <t>anti-IgG</t> antibodies were used as a secondary reagent. (4) Streptavidin-coated MNPs bind to the biotinylated detection antibodies, and the respective sensor detects stray field from the bound MNPs.
    Biotin Sp Affinipure Goat Anti Human Igg Fcγ Fragment Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp affinipure goat anti human igg fcγ fragment specific/product/Jackson Immuno
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure goat anti human igg fcγ fragment specific - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    Plasma cells, B cells and macrophages in cardiac allografts with CAV (A) Left panel: Representative image of a tissue section from a CAV patient stained for CD20 + B cells (pink) and CD138 + plasma cells (brown). Middle and right panels: Immunofluorescence staining of consecutive formalin-fixed paraffin-embedded (FFPE) tissue sections from a representative CAV sample showing plasma cell–rich infiltrates adjacent to the coronary artery of a heart explant with CAV. CD138 + plasma cells (red) co-stained for secreted IgM or IgG (blue). Nuclei are shown in gray. (B) Representative staining of cardiac explant sections with CAV showing CD68 + macrophages (brown) and CD20 + B cells (pink, left panel) or CD138 + plasma cells (pink, middle panel). Right panel: Venn diagram representation of the type of infiltrates observed in the 56 CAV explants: B cell; plasma cell; and macrophage. The number of specimens with evidence of infiltrate is indicated for each cell type. One unique sample had no infiltration. (C) Left and middle left panel: Representative immunofluorescence staining of consecutive FFPE tissue sections of a cardiac explant with CAV for M2 macrophage marker CD163 (left) or M1 marker iNOS (middle left) in combination with CD68. Middle right panel: Immunofluorescence staining of an FFPE tissue section of a cardiac explant with CAV for M2 macrophage markers CD206 and CD163. Right panel: Representative immunofluorescence staining of an FFPE tissue section of a cardiac explant with CAV for IL-10 and M2 macrophage marker CD206.

    Journal: The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation

    Article Title: Prevalence of polyreactive innate clones among graft-infiltrating B cells in human cardiac allograft vasculopathy

    doi: 10.1016/j.healun.2017.09.011

    Figure Lengend Snippet: Plasma cells, B cells and macrophages in cardiac allografts with CAV (A) Left panel: Representative image of a tissue section from a CAV patient stained for CD20 + B cells (pink) and CD138 + plasma cells (brown). Middle and right panels: Immunofluorescence staining of consecutive formalin-fixed paraffin-embedded (FFPE) tissue sections from a representative CAV sample showing plasma cell–rich infiltrates adjacent to the coronary artery of a heart explant with CAV. CD138 + plasma cells (red) co-stained for secreted IgM or IgG (blue). Nuclei are shown in gray. (B) Representative staining of cardiac explant sections with CAV showing CD68 + macrophages (brown) and CD20 + B cells (pink, left panel) or CD138 + plasma cells (pink, middle panel). Right panel: Venn diagram representation of the type of infiltrates observed in the 56 CAV explants: B cell; plasma cell; and macrophage. The number of specimens with evidence of infiltrate is indicated for each cell type. One unique sample had no infiltration. (C) Left and middle left panel: Representative immunofluorescence staining of consecutive FFPE tissue sections of a cardiac explant with CAV for M2 macrophage marker CD163 (left) or M1 marker iNOS (middle left) in combination with CD68. Middle right panel: Immunofluorescence staining of an FFPE tissue section of a cardiac explant with CAV for M2 macrophage markers CD206 and CD163. Right panel: Representative immunofluorescence staining of an FFPE tissue section of a cardiac explant with CAV for IL-10 and M2 macrophage marker CD206.

    Article Snippet: Antibody binding was revealed with HRP-conjugated goat anti-human IgG or IgM (Jackson ImmunoResearch Laboratories, West Grove, PA), and developed using 3,3′,5,5′-tetramethylbenzidine (TMB; Life Technologies).

    Techniques: Staining, Immunofluorescence, Formalin-fixed Paraffin-Embedded, Marker

    Comparison of dermal and epidermal foreskin Igs to blood Igs Foreskin and plasma samples from 17 participants were compared by calculating the ratios of each antibody concentration normalized to Human Serum Albumin (HSA) in each sample. HSA, A) IgA, B) IgG1, C) IgG2, D) IgG3, E) IgG4, F) IgM, and G) IgE were measured by multiplex bead array (MBA). Samples under the level of detection are graphed at the bottom of the axis. Only significant differences in Wilcoxon post-test after Bonferroni correction for eight comparisons are reported in the figure.

    Journal: Mucosal immunology

    Article Title: In Men at Risk of HIV Infection, IgM, IgG1, IgG3 and IgA Reach the Human Foreskin Epidermis

    doi: 10.1038/mi.2015.103

    Figure Lengend Snippet: Comparison of dermal and epidermal foreskin Igs to blood Igs Foreskin and plasma samples from 17 participants were compared by calculating the ratios of each antibody concentration normalized to Human Serum Albumin (HSA) in each sample. HSA, A) IgA, B) IgG1, C) IgG2, D) IgG3, E) IgG4, F) IgM, and G) IgE were measured by multiplex bead array (MBA). Samples under the level of detection are graphed at the bottom of the axis. Only significant differences in Wilcoxon post-test after Bonferroni correction for eight comparisons are reported in the figure.

    Article Snippet: For IgM/IgG staining, goat anti-human IgG and IgM (1:5,000; Jackson Immunoresearch) or goat isotype (3.4μg/ml) was added for 60 min followed by CSA II amplification for readout on FITC.

    Techniques: Concentration Assay, Multiplex Assay

    The foreskin contains more IgG2 and less IgA than colonic mucosa Twenty paired foreskin and colonic tissue samples were homogenized and assayed by multiplex bead array for A) IgA, B) IgG1, C) IgG2, D) IgG3, and E) IgG4. All Ig concentrations were normalized to μg of protein in the tissue biopsy. Bars indicate median with IQR for each tissue. Lysate buffer designed for homogenization of skin samples prevented detection of IgM and IgE standards, and thus their quantitation was not included in the figure. Only significant differences in Wilcoxon post-test after Bonferroni correction for three comparisons are reported in the figure.

    Journal: Mucosal immunology

    Article Title: In Men at Risk of HIV Infection, IgM, IgG1, IgG3 and IgA Reach the Human Foreskin Epidermis

    doi: 10.1038/mi.2015.103

    Figure Lengend Snippet: The foreskin contains more IgG2 and less IgA than colonic mucosa Twenty paired foreskin and colonic tissue samples were homogenized and assayed by multiplex bead array for A) IgA, B) IgG1, C) IgG2, D) IgG3, and E) IgG4. All Ig concentrations were normalized to μg of protein in the tissue biopsy. Bars indicate median with IQR for each tissue. Lysate buffer designed for homogenization of skin samples prevented detection of IgM and IgE standards, and thus their quantitation was not included in the figure. Only significant differences in Wilcoxon post-test after Bonferroni correction for three comparisons are reported in the figure.

    Article Snippet: For IgM/IgG staining, goat anti-human IgG and IgM (1:5,000; Jackson Immunoresearch) or goat isotype (3.4μg/ml) was added for 60 min followed by CSA II amplification for readout on FITC.

    Techniques: Multiplex Assay, Homogenization, Quantitation Assay

    Most Ig/HSA ratios in the foreskin dermis do not correlate with those in blood Correlation between 17 foreskin dermal antibody HSA ratios and those in paired blood were analyzed using Spearman’s Correlation. HSA, A) IgA, B) IgG1, C) IgG2, D) IgG3, E) IgG4, F) IgM, and G) IgE were measured via multiplex bead array (MBA). Inner dermis samples are labeled with filled black circles; outer dermis samples are labeled with open black squares. Spearman’s p values reported in the figure are shown after Bonferroni correction for seven comparisons.

    Journal: Mucosal immunology

    Article Title: In Men at Risk of HIV Infection, IgM, IgG1, IgG3 and IgA Reach the Human Foreskin Epidermis

    doi: 10.1038/mi.2015.103

    Figure Lengend Snippet: Most Ig/HSA ratios in the foreskin dermis do not correlate with those in blood Correlation between 17 foreskin dermal antibody HSA ratios and those in paired blood were analyzed using Spearman’s Correlation. HSA, A) IgA, B) IgG1, C) IgG2, D) IgG3, E) IgG4, F) IgM, and G) IgE were measured via multiplex bead array (MBA). Inner dermis samples are labeled with filled black circles; outer dermis samples are labeled with open black squares. Spearman’s p values reported in the figure are shown after Bonferroni correction for seven comparisons.

    Article Snippet: For IgM/IgG staining, goat anti-human IgG and IgM (1:5,000; Jackson Immunoresearch) or goat isotype (3.4μg/ml) was added for 60 min followed by CSA II amplification for readout on FITC.

    Techniques: Multiplex Assay, Labeling

    Foreskin tissue contains antibody secreting cells A–B) Real-time PCR comparing 20 paired colonic biopsies, blood, and inner and outer foreskin was used to quantitate mRNA transcription with a primer that amplifies A) IgM, IgG1, IgG3, and IgG4 (IgMG134); and B) IgA; normalized to TATA-box binding protein (TBBP). Only significant differences in Wilcoxon post-test after Bonferroni correction for six comparisons are reported beneath each graph. Individual measurements are represented by circles. Box plots show the median and IQR, with whiskers extending to the range of the data for each tissue. C) Representative 20× magnification immunofluorescence microscopy images of paired inner and outer foreskin sections stained with DAPI nuclear counterstain (pseudocolored blue), CD138 (pseudocolored green) and IgG or their isotype control (both pseudocolored red). D) Selected 20× magnification immunofluorescence microscopy images of paired inner and outer foreskin sections stained with DAPI nuclear counterstain (pseudocolored blue), CD138 (pseudocolored green) and IgA or their isotype control (pseudocolored red). In C) and D), we analyzed ~2.6mm 2 sections from 18 individuals, but photos represent only 1/200 of the area used to quantitate ASCs.

    Journal: Mucosal immunology

    Article Title: In Men at Risk of HIV Infection, IgM, IgG1, IgG3 and IgA Reach the Human Foreskin Epidermis

    doi: 10.1038/mi.2015.103

    Figure Lengend Snippet: Foreskin tissue contains antibody secreting cells A–B) Real-time PCR comparing 20 paired colonic biopsies, blood, and inner and outer foreskin was used to quantitate mRNA transcription with a primer that amplifies A) IgM, IgG1, IgG3, and IgG4 (IgMG134); and B) IgA; normalized to TATA-box binding protein (TBBP). Only significant differences in Wilcoxon post-test after Bonferroni correction for six comparisons are reported beneath each graph. Individual measurements are represented by circles. Box plots show the median and IQR, with whiskers extending to the range of the data for each tissue. C) Representative 20× magnification immunofluorescence microscopy images of paired inner and outer foreskin sections stained with DAPI nuclear counterstain (pseudocolored blue), CD138 (pseudocolored green) and IgG or their isotype control (both pseudocolored red). D) Selected 20× magnification immunofluorescence microscopy images of paired inner and outer foreskin sections stained with DAPI nuclear counterstain (pseudocolored blue), CD138 (pseudocolored green) and IgA or their isotype control (pseudocolored red). In C) and D), we analyzed ~2.6mm 2 sections from 18 individuals, but photos represent only 1/200 of the area used to quantitate ASCs.

    Article Snippet: For IgM/IgG staining, goat anti-human IgG and IgM (1:5,000; Jackson Immunoresearch) or goat isotype (3.4μg/ml) was added for 60 min followed by CSA II amplification for readout on FITC.

    Techniques: Real-time Polymerase Chain Reaction, Binding Assay, Immunofluorescence, Microscopy, Staining

    Keratin-specific autoantibody titers within tertiles of cord blood-mercury and cord blood-PCBs. Cord blood-mercury and -PCB values were separated into three groups of low, middle, and high values. Distributions of the IgM and IgG keratin-specific autoantibody

    Journal: Toxicological Sciences

    Article Title: Autoantibodies Associated with Prenatal and Childhood Exposure to Environmental Chemicals in Faroese Children

    doi: 10.1093/toxsci/kfu163

    Figure Lengend Snippet: Keratin-specific autoantibody titers within tertiles of cord blood-mercury and cord blood-PCBs. Cord blood-mercury and -PCB values were separated into three groups of low, middle, and high values. Distributions of the IgM and IgG keratin-specific autoantibody

    Article Snippet: Alkaline phosphate goat anti-human IgG or IgM (Jackson Immunoresearch, West Grover, PA), at a 1:3000 dilution, was added to each well and the plates were incubated for 1 h. Following three washes with skim milk-Tween-80 solution and two washes with 10 mM Tris, the alkaline phosphatase substrate (p-nitrophenyl-phosphate, Bio-Rad, Richmond, CA) was added.

    Techniques:

    GMR biosensor autoantigen microarrays. ( a ) Optical images of a GMR biosensor chip and a cartridge with a reaction well (left). The sensor chip measures 10 × 12 mm and consists of an array of 8 × 10 sensors (total 80 sensors). Each sensor size is 100 × 100 μm (right). ( b ) A schematic of assaying antibody reactivity to autoantigens (not to scale). (1) Autoantigens were printed on the surface of the chip’s sensors. (2) The sample was added to the reaction well, allowing antibodies to bind to their corresponding antigens. (3) After washing, species-specific, biotinylated anti-IgG antibodies were used as a secondary reagent. (4) Streptavidin-coated MNPs bind to the biotinylated detection antibodies, and the respective sensor detects stray field from the bound MNPs.

    Journal: Scientific Reports

    Article Title: Multiplex giant magnetoresistive biosensor microarrays identify interferon-associated autoantibodies in systemic lupus erythematosus

    doi: 10.1038/srep27623

    Figure Lengend Snippet: GMR biosensor autoantigen microarrays. ( a ) Optical images of a GMR biosensor chip and a cartridge with a reaction well (left). The sensor chip measures 10 × 12 mm and consists of an array of 8 × 10 sensors (total 80 sensors). Each sensor size is 100 × 100 μm (right). ( b ) A schematic of assaying antibody reactivity to autoantigens (not to scale). (1) Autoantigens were printed on the surface of the chip’s sensors. (2) The sample was added to the reaction well, allowing antibodies to bind to their corresponding antigens. (3) After washing, species-specific, biotinylated anti-IgG antibodies were used as a secondary reagent. (4) Streptavidin-coated MNPs bind to the biotinylated detection antibodies, and the respective sensor detects stray field from the bound MNPs.

    Article Snippet: The microarrays were then washed with rinsing buffer and incubated for 1 hour with biotinylated anti-human IgG (109-065-098, Jackson ImmunoResearch, PA, USA; at 100 ng/mL), anti-mouse IgG (ab98711, Abcam, MA, USA; at 50 ng/mL), or anti-rabbit IgG (ab97198, Abcam; 100 ng/mL) secondary antibodies.

    Techniques: Chromatin Immunoprecipitation