biotin sp affinipure goat anti human igg fcγ fragment specific  (Jackson Immuno)

 
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    Name:
    Biotin SP AffiniPure Goat Anti Human IgG Fcγ Fragment Specific
    Description:
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with the Fc portion of human IgG heavy chain but not with the Fab portion of human IgG No antibody was detected against human IgM or IgA or against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    Catalog Number:
    109-065-008
    Price:
    134
    Purity:
    The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
    Conjugate:
    Biotin SP long spacer
    Size:
    ml
    Category:
    Secondary Antibody
    Source:
    Goat
    Quantity:
    2 0
    Buy from Supplier


    Structured Review

    Jackson Immuno biotin sp affinipure goat anti human igg fcγ fragment specific
    Biotin SP AffiniPure Goat Anti Human IgG Fcγ Fragment Specific
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with the Fc portion of human IgG heavy chain but not with the Fab portion of human IgG No antibody was detected against human IgM or IgA or against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    https://www.bioz.com/result/biotin sp affinipure goat anti human igg fcγ fragment specific/product/Jackson Immuno
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure goat anti human igg fcγ fragment specific - by Bioz Stars, 2020-04
    99/100 stars

    Images

    1) Product Images from "Multiplex giant magnetoresistive biosensor microarrays identify interferon-associated autoantibodies in systemic lupus erythematosus"

    Article Title: Multiplex giant magnetoresistive biosensor microarrays identify interferon-associated autoantibodies in systemic lupus erythematosus

    Journal: Scientific Reports

    doi: 10.1038/srep27623

    GMR biosensor autoantigen microarrays. ( a ) Optical images of a GMR biosensor chip and a cartridge with a reaction well (left). The sensor chip measures 10 × 12 mm and consists of an array of 8 × 10 sensors (total 80 sensors). Each sensor size is 100 × 100 μm (right). ( b ) A schematic of assaying antibody reactivity to autoantigens (not to scale). (1) Autoantigens were printed on the surface of the chip’s sensors. (2) The sample was added to the reaction well, allowing antibodies to bind to their corresponding antigens. (3) After washing, species-specific, biotinylated anti-IgG antibodies were used as a secondary reagent. (4) Streptavidin-coated MNPs bind to the biotinylated detection antibodies, and the respective sensor detects stray field from the bound MNPs.
    Figure Legend Snippet: GMR biosensor autoantigen microarrays. ( a ) Optical images of a GMR biosensor chip and a cartridge with a reaction well (left). The sensor chip measures 10 × 12 mm and consists of an array of 8 × 10 sensors (total 80 sensors). Each sensor size is 100 × 100 μm (right). ( b ) A schematic of assaying antibody reactivity to autoantigens (not to scale). (1) Autoantigens were printed on the surface of the chip’s sensors. (2) The sample was added to the reaction well, allowing antibodies to bind to their corresponding antigens. (3) After washing, species-specific, biotinylated anti-IgG antibodies were used as a secondary reagent. (4) Streptavidin-coated MNPs bind to the biotinylated detection antibodies, and the respective sensor detects stray field from the bound MNPs.

    Techniques Used: Chromatin Immunoprecipitation

    Related Articles

    Microscopy:

    Article Title: nev (cyfip2) IS REQUIRED FOR RETINAL LAMINATION AND AXON GUIDANCE IN THE ZEBRAFISH RETINOTECTAL SYSTEM
    Article Snippet: For staining with EphB2-Fc or ephrin-B1-Fc fusion proteins ( ) embryos were fixed in ice-cold 100% MeOH, washed in PBST, then incubated in the affinity reagent (10ng/μl; R & D Systems catalog# 467-B2-200 and 473-EB-200) overnight at 4°C, washed again in PBS, fixed again in 4% PFA, washed in PBST, then incubated in biotin-conjugated goat anti-human Fc (1:200; Jackson Immunoresearch catalog# 109-065-098) and stained with the Vectastain ABC kit (Vector Laboratories). .. Stained larvae were genotyped for nev , prepared for sectioning or cleared in 100% glycerol, and photographed using an Olympus (BX50WI) compound microscope with an Olympus Magnafire SP camera.

    IgA Assay:

    Article Title: Lipopolysaccharide-specific Memory B Cell Responses to an Attenuated Live Cholera Vaccine are Associated with Protection Against Vibrio cholerae Infection
    Article Snippet: PBMC were cultured in ELISPOT plates for 3 hr (IgG assay) or 18–22 hr (IgA assay) at 37 °C, 5% CO2 . .. After culture, wells in the IgG plates were washed with PBS/0.05% Tween-20 (Sigma-Aldrich) and biotin-conjugated anti-human IgG antibody (Jackson ImmunoResearch # 109-065-098, 1:1000) was added and allowed to incubate overnight at 4 °C.

    Incubation:

    Article Title: Lipopolysaccharide-specific Memory B Cell Responses to an Attenuated Live Cholera Vaccine are Associated with Protection Against Vibrio cholerae Infection
    Article Snippet: After culture, wells in the IgG plates were washed with PBS/0.05% Tween-20 (Sigma-Aldrich) and biotin-conjugated anti-human IgG antibody (Jackson ImmunoResearch # 109-065-098, 1:1000) was added and allowed to incubate overnight at 4 °C. .. For IgA plates, wells were washed and biotin-conjugated anti-human IgA (BD Biosciences #555884; 1:1000) was added and incubated for 1–2 hr at room temperature.

    Article Title: nev (cyfip2) IS REQUIRED FOR RETINAL LAMINATION AND AXON GUIDANCE IN THE ZEBRAFISH RETINOTECTAL SYSTEM
    Article Snippet: .. For staining with EphB2-Fc or ephrin-B1-Fc fusion proteins ( ) embryos were fixed in ice-cold 100% MeOH, washed in PBST, then incubated in the affinity reagent (10ng/μl; R & D Systems catalog# 467-B2-200 and 473-EB-200) overnight at 4°C, washed again in PBS, fixed again in 4% PFA, washed in PBST, then incubated in biotin-conjugated goat anti-human Fc (1:200; Jackson Immunoresearch catalog# 109-065-098) and stained with the Vectastain ABC kit (Vector Laboratories). .. Stained larvae were genotyped for nev , prepared for sectioning or cleared in 100% glycerol, and photographed using an Olympus (BX50WI) compound microscope with an Olympus Magnafire SP camera.

    Article Title: Multiplex giant magnetoresistive biosensor microarrays identify interferon-associated autoantibodies in systemic lupus erythematosus
    Article Snippet: .. The microarrays were then washed with rinsing buffer and incubated for 1 hour with biotinylated anti-human IgG (109-065-098, Jackson ImmunoResearch, PA, USA; at 100 ng/mL), anti-mouse IgG (ab98711, Abcam, MA, USA; at 50 ng/mL), or anti-rabbit IgG (ab97198, Abcam; 100 ng/mL) secondary antibodies. .. For analysis of serum samples with peptides, streptavidin (5 mg/mL in PBS) was printed on the sensors of a GMR sensor chip, along with BSA and purified human IgG (Sigma) controls.

    Article Title: Mapping an Adeno-associated Virus 9-Specific Neutralizing Epitope To Develop Next-Generation Gene Delivery Vectors
    Article Snippet: We then incubated the hybridoma supernatant, purified mAb, serum, or plasma (diluted in 0.75% BSA in PBS) at 37°C for 1 h, followed by a triple wash with 300 μl of PBS plus 0.05% Tween. .. The human and nonhuman primate samples were then detected using 1:10,000 (diluted in PBS) goat anti-human IgG biotin-SP (catalog no. 109-065-098; Jackson ImmunoResearch, Inc., West Grove, PA) at room temperature for 1 h, followed by a triple wash of 300 μl of PBS plus 0.05% Tween and 1:30,000 (diluted in PBS) unconjugated streptavidin (catalog no. 016-000-084; Jackson ImmunoResearch) at room temperature for 1 h (followed by a 3× wash with 300 μl of PBS plus 0.05% Tween).

    Cell Culture:

    Article Title: Lipopolysaccharide-specific Memory B Cell Responses to an Attenuated Live Cholera Vaccine are Associated with Protection Against Vibrio cholerae Infection
    Article Snippet: PBMC were cultured in ELISPOT plates for 3 hr (IgG assay) or 18–22 hr (IgA assay) at 37 °C, 5% CO2 . .. After culture, wells in the IgG plates were washed with PBS/0.05% Tween-20 (Sigma-Aldrich) and biotin-conjugated anti-human IgG antibody (Jackson ImmunoResearch # 109-065-098, 1:1000) was added and allowed to incubate overnight at 4 °C.

    Purification:

    Article Title: Multiplex giant magnetoresistive biosensor microarrays identify interferon-associated autoantibodies in systemic lupus erythematosus
    Article Snippet: The microarrays were then washed with rinsing buffer and incubated for 1 hour with biotinylated anti-human IgG (109-065-098, Jackson ImmunoResearch, PA, USA; at 100 ng/mL), anti-mouse IgG (ab98711, Abcam, MA, USA; at 50 ng/mL), or anti-rabbit IgG (ab97198, Abcam; 100 ng/mL) secondary antibodies. .. For analysis of serum samples with peptides, streptavidin (5 mg/mL in PBS) was printed on the sensors of a GMR sensor chip, along with BSA and purified human IgG (Sigma) controls.

    Article Title: Mapping an Adeno-associated Virus 9-Specific Neutralizing Epitope To Develop Next-Generation Gene Delivery Vectors
    Article Snippet: We then incubated the hybridoma supernatant, purified mAb, serum, or plasma (diluted in 0.75% BSA in PBS) at 37°C for 1 h, followed by a triple wash with 300 μl of PBS plus 0.05% Tween. .. The human and nonhuman primate samples were then detected using 1:10,000 (diluted in PBS) goat anti-human IgG biotin-SP (catalog no. 109-065-098; Jackson ImmunoResearch, Inc., West Grove, PA) at room temperature for 1 h, followed by a triple wash of 300 μl of PBS plus 0.05% Tween and 1:30,000 (diluted in PBS) unconjugated streptavidin (catalog no. 016-000-084; Jackson ImmunoResearch) at room temperature for 1 h (followed by a 3× wash with 300 μl of PBS plus 0.05% Tween).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Mapping an Adeno-associated Virus 9-Specific Neutralizing Epitope To Develop Next-Generation Gene Delivery Vectors
    Article Snippet: Paragraph title: AAV capsid ELISA. ... The human and nonhuman primate samples were then detected using 1:10,000 (diluted in PBS) goat anti-human IgG biotin-SP (catalog no. 109-065-098; Jackson ImmunoResearch, Inc., West Grove, PA) at room temperature for 1 h, followed by a triple wash of 300 μl of PBS plus 0.05% Tween and 1:30,000 (diluted in PBS) unconjugated streptavidin (catalog no. 016-000-084; Jackson ImmunoResearch) at room temperature for 1 h (followed by a 3× wash with 300 μl of PBS plus 0.05% Tween).

    Generated:

    Article Title: nev (cyfip2) IS REQUIRED FOR RETINAL LAMINATION AND AXON GUIDANCE IN THE ZEBRAFISH RETINOTECTAL SYSTEM
    Article Snippet: In situ hybridization was carried out according to . cyfip2 antisense riboprobes were generated from a cDNA clone (GenBank accession no. ). ephrin-B2a , EphB3 , aldh1a2 , TAG-1 , and tbx5a plasmids were generous gifts from S. Wilson, H. Grandel, J. Kuwada, and D. Yelon, respectively. .. For staining with EphB2-Fc or ephrin-B1-Fc fusion proteins ( ) embryos were fixed in ice-cold 100% MeOH, washed in PBST, then incubated in the affinity reagent (10ng/μl; R & D Systems catalog# 467-B2-200 and 473-EB-200) overnight at 4°C, washed again in PBS, fixed again in 4% PFA, washed in PBST, then incubated in biotin-conjugated goat anti-human Fc (1:200; Jackson Immunoresearch catalog# 109-065-098) and stained with the Vectastain ABC kit (Vector Laboratories).

    Enzyme-linked Immunospot:

    Article Title: Lipopolysaccharide-specific Memory B Cell Responses to an Attenuated Live Cholera Vaccine are Associated with Protection Against Vibrio cholerae Infection
    Article Snippet: PBMC were cultured in ELISPOT plates for 3 hr (IgG assay) or 18–22 hr (IgA assay) at 37 °C, 5% CO2 . .. After culture, wells in the IgG plates were washed with PBS/0.05% Tween-20 (Sigma-Aldrich) and biotin-conjugated anti-human IgG antibody (Jackson ImmunoResearch # 109-065-098, 1:1000) was added and allowed to incubate overnight at 4 °C.

    Blocking Assay:

    Article Title: Multiplex giant magnetoresistive biosensor microarrays identify interferon-associated autoantibodies in systemic lupus erythematosus
    Article Snippet: After blocking, the microarrays were washed with rinsing buffer and probed with diluted serum (1/200 in rinsing buffer) or commercial antibodies for 2 hours (at specified concentrations in rinsing buffer) with agitation at room temperature. .. The microarrays were then washed with rinsing buffer and incubated for 1 hour with biotinylated anti-human IgG (109-065-098, Jackson ImmunoResearch, PA, USA; at 100 ng/mL), anti-mouse IgG (ab98711, Abcam, MA, USA; at 50 ng/mL), or anti-rabbit IgG (ab97198, Abcam; 100 ng/mL) secondary antibodies.

    Staining:

    Article Title: nev (cyfip2) IS REQUIRED FOR RETINAL LAMINATION AND AXON GUIDANCE IN THE ZEBRAFISH RETINOTECTAL SYSTEM
    Article Snippet: .. For staining with EphB2-Fc or ephrin-B1-Fc fusion proteins ( ) embryos were fixed in ice-cold 100% MeOH, washed in PBST, then incubated in the affinity reagent (10ng/μl; R & D Systems catalog# 467-B2-200 and 473-EB-200) overnight at 4°C, washed again in PBS, fixed again in 4% PFA, washed in PBST, then incubated in biotin-conjugated goat anti-human Fc (1:200; Jackson Immunoresearch catalog# 109-065-098) and stained with the Vectastain ABC kit (Vector Laboratories). .. Stained larvae were genotyped for nev , prepared for sectioning or cleared in 100% glycerol, and photographed using an Olympus (BX50WI) compound microscope with an Olympus Magnafire SP camera.

    Recombinant:

    Article Title: Improved detection of prostate cancer using a magneto-nanosensor assay for serum circulating autoantibodies
    Article Snippet: Paragraph title: Recombinant proteins & antibodies ... Detecting IgG: The anti-human IgG biotinylated antibody (109-065-098) was obtained from Jackson Immuno Research.

    Chromatin Immunoprecipitation:

    Article Title: Multiplex giant magnetoresistive biosensor microarrays identify interferon-associated autoantibodies in systemic lupus erythematosus
    Article Snippet: GMR biosensor autoantigen microarrays The autoantigens shown in were printed at 0.2 mg/mL (in PBS) on the sensors of a GMR sensor chip in replicates of 4 using a non-contact microarrayer (sciFLEXARRAYER, Scienion, NJ, USA). .. The microarrays were then washed with rinsing buffer and incubated for 1 hour with biotinylated anti-human IgG (109-065-098, Jackson ImmunoResearch, PA, USA; at 100 ng/mL), anti-mouse IgG (ab98711, Abcam, MA, USA; at 50 ng/mL), or anti-rabbit IgG (ab97198, Abcam; 100 ng/mL) secondary antibodies.

    In Situ Hybridization:

    Article Title: nev (cyfip2) IS REQUIRED FOR RETINAL LAMINATION AND AXON GUIDANCE IN THE ZEBRAFISH RETINOTECTAL SYSTEM
    Article Snippet: Paragraph title: In situ hybridization and Fc fusion staining ... For staining with EphB2-Fc or ephrin-B1-Fc fusion proteins ( ) embryos were fixed in ice-cold 100% MeOH, washed in PBST, then incubated in the affinity reagent (10ng/μl; R & D Systems catalog# 467-B2-200 and 473-EB-200) overnight at 4°C, washed again in PBS, fixed again in 4% PFA, washed in PBST, then incubated in biotin-conjugated goat anti-human Fc (1:200; Jackson Immunoresearch catalog# 109-065-098) and stained with the Vectastain ABC kit (Vector Laboratories).

    Plasmid Preparation:

    Article Title: Lipopolysaccharide-specific Memory B Cell Responses to an Attenuated Live Cholera Vaccine are Associated with Protection Against Vibrio cholerae Infection
    Article Snippet: After culture, wells in the IgG plates were washed with PBS/0.05% Tween-20 (Sigma-Aldrich) and biotin-conjugated anti-human IgG antibody (Jackson ImmunoResearch # 109-065-098, 1:1000) was added and allowed to incubate overnight at 4 °C. .. Wells were developed for detection of spot forming cells (SFC) by incubation with Vectastain-Avidin-Peroxidase-Complex solution (Vector Lab # PK6100) for 1 hr at room temperature followed by washing and incubation with TrueBlue peroxidase (KPL #50-78-02) for 5–6 min at room temperature [ ].

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  • Bioz Stars
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  • 99
    Jackson Immuno biotin sp affinipure goat anti human igg fcγ fragment specific
    GMR biosensor autoantigen microarrays. ( a ) Optical images of a GMR biosensor chip and a cartridge with a reaction well (left). The sensor chip measures 10 × 12 mm and consists of an array of 8 × 10 sensors (total 80 sensors). Each sensor size is 100 × 100 μm (right). ( b ) A schematic of assaying antibody reactivity to autoantigens (not to scale). (1) Autoantigens were printed on the surface of the chip’s sensors. (2) The sample was added to the reaction well, allowing antibodies to bind to their corresponding antigens. (3) After washing, species-specific, <t>biotinylated</t> <t>anti-IgG</t> antibodies were used as a secondary reagent. (4) Streptavidin-coated MNPs bind to the biotinylated detection antibodies, and the respective sensor detects stray field from the bound MNPs.
    Biotin Sp Affinipure Goat Anti Human Igg Fcγ Fragment Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp affinipure goat anti human igg fcγ fragment specific/product/Jackson Immuno
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure goat anti human igg fcγ fragment specific - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Jackson Immuno biotin sp affinipure f ab 2 fragment goat anti human igg fcγ fragment specific
    IdeS cleaves IgG-type, but not IgM-type, of BCR on B cells. ( A ) Flow cytometry analysis of cells stained with <t>biotinylated</t> anti-Fab Ab, followed by streptavidin-allophycocyanin, after treatment of Nu-DUL-1 cells (IgG-type) and Daudi cells (IgM-type) with
    Biotin Sp Affinipure F Ab 2 Fragment Goat Anti Human Igg Fcγ Fragment Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp affinipure f ab 2 fragment goat anti human igg fcγ fragment specific/product/Jackson Immuno
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure f ab 2 fragment goat anti human igg fcγ fragment specific - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    GMR biosensor autoantigen microarrays. ( a ) Optical images of a GMR biosensor chip and a cartridge with a reaction well (left). The sensor chip measures 10 × 12 mm and consists of an array of 8 × 10 sensors (total 80 sensors). Each sensor size is 100 × 100 μm (right). ( b ) A schematic of assaying antibody reactivity to autoantigens (not to scale). (1) Autoantigens were printed on the surface of the chip’s sensors. (2) The sample was added to the reaction well, allowing antibodies to bind to their corresponding antigens. (3) After washing, species-specific, biotinylated anti-IgG antibodies were used as a secondary reagent. (4) Streptavidin-coated MNPs bind to the biotinylated detection antibodies, and the respective sensor detects stray field from the bound MNPs.

    Journal: Scientific Reports

    Article Title: Multiplex giant magnetoresistive biosensor microarrays identify interferon-associated autoantibodies in systemic lupus erythematosus

    doi: 10.1038/srep27623

    Figure Lengend Snippet: GMR biosensor autoantigen microarrays. ( a ) Optical images of a GMR biosensor chip and a cartridge with a reaction well (left). The sensor chip measures 10 × 12 mm and consists of an array of 8 × 10 sensors (total 80 sensors). Each sensor size is 100 × 100 μm (right). ( b ) A schematic of assaying antibody reactivity to autoantigens (not to scale). (1) Autoantigens were printed on the surface of the chip’s sensors. (2) The sample was added to the reaction well, allowing antibodies to bind to their corresponding antigens. (3) After washing, species-specific, biotinylated anti-IgG antibodies were used as a secondary reagent. (4) Streptavidin-coated MNPs bind to the biotinylated detection antibodies, and the respective sensor detects stray field from the bound MNPs.

    Article Snippet: The microarrays were then washed with rinsing buffer and incubated for 1 hour with biotinylated anti-human IgG (109-065-098, Jackson ImmunoResearch, PA, USA; at 100 ng/mL), anti-mouse IgG (ab98711, Abcam, MA, USA; at 50 ng/mL), or anti-rabbit IgG (ab97198, Abcam; 100 ng/mL) secondary antibodies.

    Techniques: Chromatin Immunoprecipitation

    IdeS cleaves IgG-type, but not IgM-type, of BCR on B cells. ( A ) Flow cytometry analysis of cells stained with biotinylated anti-Fab Ab, followed by streptavidin-allophycocyanin, after treatment of Nu-DUL-1 cells (IgG-type) and Daudi cells (IgM-type) with

    Journal: The Journal of Immunology Author Choice

    Article Title: The Bacterial Enzyme IdeS Cleaves the IgG-Type of B Cell Receptor (BCR), Abolishes BCR-Mediated Cell Signaling, and Inhibits Memory B Cell Activation

    doi: 10.4049/jimmunol.1501929

    Figure Lengend Snippet: IdeS cleaves IgG-type, but not IgM-type, of BCR on B cells. ( A ) Flow cytometry analysis of cells stained with biotinylated anti-Fab Ab, followed by streptavidin-allophycocyanin, after treatment of Nu-DUL-1 cells (IgG-type) and Daudi cells (IgM-type) with

    Article Snippet: A total of 10 μg/ml biotinylated anti–CH1-IgG (BAC, cat. no. 7103202100) was used for detection of the F(ab′)2 portion of IgG, and 0.5 μg/ml biotinylated goat anti-human Fc-specific F(ab′)2 fragment (Jackson ImmunoResearch, cat. no. 109-066-098) was used for detection of the Fc portion of IgG.

    Techniques: Flow Cytometry, Cytometry, Staining