biotin sp affinipure f  (Jackson Immuno)

 
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    Name:
    Biotin SP AffiniPure F ab 2 Fragment Donkey Anti Chicken IgY IgG
    Description:
    F ab 2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region F ab 2 fragments have two antigen binding Fab portions linked together by disulfide bonds and therefore they are divalent The average molecular weight is about 110 kDa They are used for specific applications such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule chicken IgY It also reacts with the light chains of other chicken immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with bovine goat guinea pig syrian hamster horse human mouse rabbit rat and sheep serum proteins but it may cross react with immunoglobulins from other species
    Catalog Number:
    703-066-155
    Price:
    137
    Purity:
    The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
    Conjugate:
    Biotin SP long spacer
    Size:
    ml
    Category:
    Secondary Antibody
    Source:
    Donkey
    Quantity:
    0 3
    Buy from Supplier


    Structured Review

    Jackson Immuno biotin sp affinipure f
    Biotin SP AffiniPure F ab 2 Fragment Donkey Anti Chicken IgY IgG
    F ab 2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region F ab 2 fragments have two antigen binding Fab portions linked together by disulfide bonds and therefore they are divalent The average molecular weight is about 110 kDa They are used for specific applications such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule chicken IgY It also reacts with the light chains of other chicken immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with bovine goat guinea pig syrian hamster horse human mouse rabbit rat and sheep serum proteins but it may cross react with immunoglobulins from other species
    https://www.bioz.com/result/biotin sp affinipure f/product/Jackson Immuno
    Average 94 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure f - by Bioz Stars, 2020-07
    94/100 stars

    Images

    Related Articles

    Transduction:

    Article Title: Fluorine-19 nuclear magnetic resonance of chimeric antigen receptor T cell biodistribution in murine cancer model
    Article Snippet: .. The T cell transduction efficacy was determined by flow cytometry at 5 and 14 days after virus addition using a primary biotin-SP-AffiniPure F(ab’)2 fragment-specific goat anti-mouse antibody (Jackson Immuno Research Laboratories, West Grove, PA) and streptavidin-PE served as the secondary antibody (BD Pharmingen, San Diego, CA). .. To label T cells for NMR cytometry, CAR T cells and untransduced T cells (control) were plated at a density of 10 million cells in 5 ml of RPMI in 6-well plates and incubated for 12 h with 10 mg/ml of PFC nanoemulsion (CS-1000 or CS-ATM DM Red, Celsense, Inc., Pittsburgh, PA).

    Article Title: Lentiviral Vector Production Titer Is Not Limited in HEK293T by Induced Intracellular Innate Immunity
    Article Snippet: .. LV-CAR transduction efficiency was assessed by flow cytometry staining cells with biotin AffiniPure F(ab’) fragment goat anti-mouse immunoglobulin IgG (Jackson Immunoresearch, West Grove, PA, USA) followed by streptavidin-APC (Biolegend, San Diego, CA). .. CXCL10 ELISA Cell culture supernatants from HEK293T cells grown in 6-well plates were assayed at 48 h post transfection for CXCL10 protein using Duoset enzyme-linked immunosorbent assay (ELISA) reagents (R & D Systems) according to the manufacturer’s instructions.

    Staining:

    Article Title: The Rate of CD4 T Cell Entry into the Lungs during Mycobacterium tuberculosis Infection Is Determined by Partial and Opposing Effects of Multiple Chemokine Receptors
    Article Snippet: .. The cells treated with human anti-KLRG1 IgG1 (kindly provided by Abcuro, Newton MA) were stained with a biotin-SP AffiniPure F(ab′)2 fragment donkey anti-human IgG, Fcγ fragment-specific antibody purchased from Jackson ImmunoResearch Laboratory (West Grove, PA) and secondarily stained with fluorochrome-labeled streptavidin. .. Samples were acquired on an LSR II Fortessa flow cytometer and analyzed using FlowJo software (BD Biosciences, San Jose, CA).

    Article Title: Lentiviral Vector Production Titer Is Not Limited in HEK293T by Induced Intracellular Innate Immunity
    Article Snippet: .. LV-CAR transduction efficiency was assessed by flow cytometry staining cells with biotin AffiniPure F(ab’) fragment goat anti-mouse immunoglobulin IgG (Jackson Immunoresearch, West Grove, PA, USA) followed by streptavidin-APC (Biolegend, San Diego, CA). .. CXCL10 ELISA Cell culture supernatants from HEK293T cells grown in 6-well plates were assayed at 48 h post transfection for CXCL10 protein using Duoset enzyme-linked immunosorbent assay (ELISA) reagents (R & D Systems) according to the manufacturer’s instructions.

    Flow Cytometry:

    Article Title: Fluorine-19 nuclear magnetic resonance of chimeric antigen receptor T cell biodistribution in murine cancer model
    Article Snippet: .. The T cell transduction efficacy was determined by flow cytometry at 5 and 14 days after virus addition using a primary biotin-SP-AffiniPure F(ab’)2 fragment-specific goat anti-mouse antibody (Jackson Immuno Research Laboratories, West Grove, PA) and streptavidin-PE served as the secondary antibody (BD Pharmingen, San Diego, CA). .. To label T cells for NMR cytometry, CAR T cells and untransduced T cells (control) were plated at a density of 10 million cells in 5 ml of RPMI in 6-well plates and incubated for 12 h with 10 mg/ml of PFC nanoemulsion (CS-1000 or CS-ATM DM Red, Celsense, Inc., Pittsburgh, PA).

    Article Title: Lentiviral Vector Production Titer Is Not Limited in HEK293T by Induced Intracellular Innate Immunity
    Article Snippet: .. LV-CAR transduction efficiency was assessed by flow cytometry staining cells with biotin AffiniPure F(ab’) fragment goat anti-mouse immunoglobulin IgG (Jackson Immunoresearch, West Grove, PA, USA) followed by streptavidin-APC (Biolegend, San Diego, CA). .. CXCL10 ELISA Cell culture supernatants from HEK293T cells grown in 6-well plates were assayed at 48 h post transfection for CXCL10 protein using Duoset enzyme-linked immunosorbent assay (ELISA) reagents (R & D Systems) according to the manufacturer’s instructions.

    Cytometry:

    Article Title: Fluorine-19 nuclear magnetic resonance of chimeric antigen receptor T cell biodistribution in murine cancer model
    Article Snippet: .. The T cell transduction efficacy was determined by flow cytometry at 5 and 14 days after virus addition using a primary biotin-SP-AffiniPure F(ab’)2 fragment-specific goat anti-mouse antibody (Jackson Immuno Research Laboratories, West Grove, PA) and streptavidin-PE served as the secondary antibody (BD Pharmingen, San Diego, CA). .. To label T cells for NMR cytometry, CAR T cells and untransduced T cells (control) were plated at a density of 10 million cells in 5 ml of RPMI in 6-well plates and incubated for 12 h with 10 mg/ml of PFC nanoemulsion (CS-1000 or CS-ATM DM Red, Celsense, Inc., Pittsburgh, PA).

    Article Title: Lentiviral Vector Production Titer Is Not Limited in HEK293T by Induced Intracellular Innate Immunity
    Article Snippet: .. LV-CAR transduction efficiency was assessed by flow cytometry staining cells with biotin AffiniPure F(ab’) fragment goat anti-mouse immunoglobulin IgG (Jackson Immunoresearch, West Grove, PA, USA) followed by streptavidin-APC (Biolegend, San Diego, CA). .. CXCL10 ELISA Cell culture supernatants from HEK293T cells grown in 6-well plates were assayed at 48 h post transfection for CXCL10 protein using Duoset enzyme-linked immunosorbent assay (ELISA) reagents (R & D Systems) according to the manufacturer’s instructions.

    Incubation:

    Article Title: Metabolic Phenotypes Of Response to Vaccination in Humans
    Article Snippet: .. The next day, the plates were washed four times with PBS, four times with PBS-T and incubated for 1.5 hr at room temperature with Biotin-SP-Affinipure F(ab′)2 donkey anti-human IgG (Jackson Immunoresearch) at 100 ng/well in PBS + 10% FCS + 0.05% Tween 20 (PBS-T-FBS). .. Plates were washed four times with PBS-T and incubated for 1.5 hr with HRP-avidin D (Vector laboratories) at 1:1000 in PBS-T-FBS.

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  • 93
    Jackson Immuno biotin sp affinipure goat anti mouse igg f ab 2 fragment specific
    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated <t>AffiniPure</t> goat anti-mouse <t>IgG,F(ab′)2</t> fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p
    Biotin Sp Affinipure Goat Anti Mouse Igg F Ab 2 Fragment Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp affinipure goat anti mouse igg f ab 2 fragment specific/product/Jackson Immuno
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure goat anti mouse igg f ab 2 fragment specific - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    92
    Jackson Immuno allophycocyanin streptavidin
    IdeS cleaves IgG-type, but not IgM-type, of BCR on B cells. ( A ) Flow cytometry analysis of cells stained with biotinylated anti-Fab Ab, followed by <t>streptavidin-allophycocyanin,</t> after treatment of Nu-DUL-1 cells (IgG-type) and Daudi cells (IgM-type) with
    Allophycocyanin Streptavidin, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/allophycocyanin streptavidin/product/Jackson Immuno
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    allophycocyanin streptavidin - by Bioz Stars, 2020-07
    92/100 stars
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    90
    Jackson Immuno hamster immunoglobulin g
    Th differentiation and B cell responses in mice deprived of functional RLTPR molecules. (A) Sorted naive CD4 + T cells (2 × 10 5 ) from mice of the specified genotype were stimulated for 5 d with anti-CD3 and -CD28 under Th1, Th2, or Th17 differentiating conditions. After 5 d of culture, the absolute number of IFN-γ + (Th1 condition), IL-4 + (Th2 condition), and IL-17 + (Th17 condition) CD4 + T cells was determined. Each dot corresponds to a mouse and the mean (horizontal bar) is indicated. (B) WT and Rltpr bas/bas mice were immunized intraperitoneally at day 0 and 14 with the T cell–dependent antigen TNP-KLH. The concentration of TNP-specific immunoglobulins of the indicated isotypes <t>(IgG2a,</t> <t>IgG2b,</t> and <t>IgG1)</t> were assessed in individual mice before and 21 d after immunization. (C) WT and Rltpr bas/bas mice were immunized with the T cell–independent antigen TNP-LPS, and the concentration of TNP-specific IgM was assessed in individual mice before and 7 d after immunization. (D) Splenic B cells from mice of the specified genotype were stimulated with F(ab)’ 2 goat anti–mouse IgM antibody in the presence or absence of anti-CD40 antibody, or LPS. After 4 d of culture, B cell proliferation was evaluated. Mean and SEM are shown. Data are representative of two independent experiments. In A–C, each dot corresponds to a mouse and the mean (horizontal bar) is indicated. **, P ≤ 0.01; ****, P ≤ 0.001; ns, nonsignificant. In D, two animals were used per genotype.
    Hamster Immunoglobulin G, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hamster immunoglobulin g/product/Jackson Immuno
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hamster immunoglobulin g - by Bioz Stars, 2020-07
    90/100 stars
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    Image Search Results


    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p

    Journal: PLoS ONE

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis

    doi: 10.1371/journal.pone.0109352

    Figure Lengend Snippet: Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p

    Article Snippet: Biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072) and PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) were used for labeling the cells.

    Techniques: Flow Cytometry, Cytometry, Cell Culture, Staining, Irradiation

    Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.

    Journal: PLoS ONE

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis

    doi: 10.1371/journal.pone.0109352

    Figure Lengend Snippet: Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.

    Article Snippet: Biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072) and PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) were used for labeling the cells.

    Techniques: Immunofluorescence, Staining, Cell Culture

    IdeS cleaves IgG-type, but not IgM-type, of BCR on B cells. ( A ) Flow cytometry analysis of cells stained with biotinylated anti-Fab Ab, followed by streptavidin-allophycocyanin, after treatment of Nu-DUL-1 cells (IgG-type) and Daudi cells (IgM-type) with

    Journal: The Journal of Immunology Author Choice

    Article Title: The Bacterial Enzyme IdeS Cleaves the IgG-Type of B Cell Receptor (BCR), Abolishes BCR-Mediated Cell Signaling, and Inhibits Memory B Cell Activation

    doi: 10.4049/jimmunol.1501929

    Figure Lengend Snippet: IdeS cleaves IgG-type, but not IgM-type, of BCR on B cells. ( A ) Flow cytometry analysis of cells stained with biotinylated anti-Fab Ab, followed by streptavidin-allophycocyanin, after treatment of Nu-DUL-1 cells (IgG-type) and Daudi cells (IgM-type) with

    Article Snippet: Cells were stained with a biotinylated Ab specific for the F(ab′)2 portion (cat. no. 109-066-097), followed by streptavidin-allophycocyanin (cat. no. 016-130-084; both from Jackson ImmunoResearch), and the cells were monitored in FL4 using an Accuri C6 flow cytometer.

    Techniques: Flow Cytometry, Cytometry, Staining

    Th differentiation and B cell responses in mice deprived of functional RLTPR molecules. (A) Sorted naive CD4 + T cells (2 × 10 5 ) from mice of the specified genotype were stimulated for 5 d with anti-CD3 and -CD28 under Th1, Th2, or Th17 differentiating conditions. After 5 d of culture, the absolute number of IFN-γ + (Th1 condition), IL-4 + (Th2 condition), and IL-17 + (Th17 condition) CD4 + T cells was determined. Each dot corresponds to a mouse and the mean (horizontal bar) is indicated. (B) WT and Rltpr bas/bas mice were immunized intraperitoneally at day 0 and 14 with the T cell–dependent antigen TNP-KLH. The concentration of TNP-specific immunoglobulins of the indicated isotypes (IgG2a, IgG2b, and IgG1) were assessed in individual mice before and 21 d after immunization. (C) WT and Rltpr bas/bas mice were immunized with the T cell–independent antigen TNP-LPS, and the concentration of TNP-specific IgM was assessed in individual mice before and 7 d after immunization. (D) Splenic B cells from mice of the specified genotype were stimulated with F(ab)’ 2 goat anti–mouse IgM antibody in the presence or absence of anti-CD40 antibody, or LPS. After 4 d of culture, B cell proliferation was evaluated. Mean and SEM are shown. Data are representative of two independent experiments. In A–C, each dot corresponds to a mouse and the mean (horizontal bar) is indicated. **, P ≤ 0.01; ****, P ≤ 0.001; ns, nonsignificant. In D, two animals were used per genotype.

    Journal: The Journal of Experimental Medicine

    Article Title: The scaffolding function of the RLTPR protein explains its essential role for CD28 co-stimulation in mouse and human T cells

    doi: 10.1084/jem.20160579

    Figure Lengend Snippet: Th differentiation and B cell responses in mice deprived of functional RLTPR molecules. (A) Sorted naive CD4 + T cells (2 × 10 5 ) from mice of the specified genotype were stimulated for 5 d with anti-CD3 and -CD28 under Th1, Th2, or Th17 differentiating conditions. After 5 d of culture, the absolute number of IFN-γ + (Th1 condition), IL-4 + (Th2 condition), and IL-17 + (Th17 condition) CD4 + T cells was determined. Each dot corresponds to a mouse and the mean (horizontal bar) is indicated. (B) WT and Rltpr bas/bas mice were immunized intraperitoneally at day 0 and 14 with the T cell–dependent antigen TNP-KLH. The concentration of TNP-specific immunoglobulins of the indicated isotypes (IgG2a, IgG2b, and IgG1) were assessed in individual mice before and 21 d after immunization. (C) WT and Rltpr bas/bas mice were immunized with the T cell–independent antigen TNP-LPS, and the concentration of TNP-specific IgM was assessed in individual mice before and 7 d after immunization. (D) Splenic B cells from mice of the specified genotype were stimulated with F(ab)’ 2 goat anti–mouse IgM antibody in the presence or absence of anti-CD40 antibody, or LPS. After 4 d of culture, B cell proliferation was evaluated. Mean and SEM are shown. Data are representative of two independent experiments. In A–C, each dot corresponds to a mouse and the mean (horizontal bar) is indicated. **, P ≤ 0.01; ****, P ≤ 0.001; ns, nonsignificant. In D, two animals were used per genotype.

    Article Snippet: CD28 internalization assay Cells were incubated for 30 min on ice with anti-CD28 (1 µg/ml; 553294; BD), followed by incubation for another 30 min on ice with biotinylated goat antibody to hamster immunoglobulin G (2 µg/ml; 107–066-142; Jackson ImmunoResearch Laboratories).

    Techniques: Mouse Assay, Functional Assay, Concentration Assay