biotin sp affinipure f ab 2 fragment goat anti human igg  (Jackson Immuno)

 
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    Name:
    Biotin SP AffiniPure F ab 2 Fragment Goat Anti Human IgG
    Description:
    F ab 2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region F ab 2 fragments have two antigen binding Fab portions linked together by disulfide bonds and therefore they are divalent The average molecular weight is about 110 kDa They are used for specific applications such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule human IgG It also reacts with the light chains of other human immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    Catalog Number:
    109-066-003
    Price:
    97
    Purity:
    The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
    Conjugate:
    Biotin SP long spacer
    Size:
    ml
    Category:
    Secondary Antibody
    Source:
    Goat
    Quantity:
    0 5
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    Structured Review

    Jackson Immuno biotin sp affinipure f ab 2 fragment goat anti human igg
    Biotin SP AffiniPure F ab 2 Fragment Goat Anti Human IgG
    F ab 2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region F ab 2 fragments have two antigen binding Fab portions linked together by disulfide bonds and therefore they are divalent The average molecular weight is about 110 kDa They are used for specific applications such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule human IgG It also reacts with the light chains of other human immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    https://www.bioz.com/result/biotin sp affinipure f ab 2 fragment goat anti human igg/product/Jackson Immuno
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure f ab 2 fragment goat anti human igg - by Bioz Stars, 2020-08
    92/100 stars

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    Incubation:

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    Article Snippet: .. Plates were then washed 3 times with 200 μL PBS, 0.05% Tween 20, and incubated at 37°C for 1 hour with biotin-labeled goat anti-human heavy chain immunoglobulin antibody (Jackson Immunoresearch, West Grove, PA, #109-066-088) diluted in PBS, 0.5% BSA, 0.05% Tween 20. .. After washing, streptavidin-HRP (R & D Systems, Minneapolis, MN) was added and incubated 1 hour at 37°C.

    Article Title: Dot enzyme‐linked immunosorbent assay strip as a screening tool for detection of autoantibody to interferon gamma in sera of suspected cases of adult‐onset immunodeficiency. Dot enzyme‐linked immunosorbent assay strip as a screening tool for detection of autoantibody to interferon gamma in sera of suspected cases of adult‐onset immunodeficiency
    Article Snippet: .. The strips were washed 5 times with 0.05% Tween‐20 in PBS, and 1 mL of 1:1000 diluted Biotin‐SP‐conjugated AffiniPure F(ab′)2 fragment donkey anti‐human IgG (Jackson ImmunoResearch) was added to each tube and incubated for 60 minutes. .. After washing, the strips were incubated in 1:1000 diluted alkaline phosphatase‐conjugated streptavidin (Vector Laboratories, Burlingame, CA, USA) for 60 minutes.

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    Jackson Immuno allophycocyanin streptavidin
    IdeS cleaves IgG-type, but not IgM-type, of BCR on B cells. ( A ) Flow cytometry analysis of cells stained with biotinylated anti-Fab Ab, followed by <t>streptavidin-allophycocyanin,</t> after treatment of Nu-DUL-1 cells (IgG-type) and Daudi cells (IgM-type) with
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    allophycocyanin streptavidin - by Bioz Stars, 2020-08
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    Jackson Immuno biotin sp affinipure f ab 2 fragment goat anti human igg fcγ fragment specific
    IdeS cleaves IgG-type, but not IgM-type, of BCR on B cells. ( A ) Flow cytometry analysis of cells stained with <t>biotinylated</t> anti-Fab Ab, followed by streptavidin-allophycocyanin, after treatment of Nu-DUL-1 cells (IgG-type) and Daudi cells (IgM-type) with
    Biotin Sp Affinipure F Ab 2 Fragment Goat Anti Human Igg Fcγ Fragment Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 89/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp affinipure f ab 2 fragment goat anti human igg fcγ fragment specific/product/Jackson Immuno
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    Image Search Results


    IdeS cleaves IgG-type, but not IgM-type, of BCR on B cells. ( A ) Flow cytometry analysis of cells stained with biotinylated anti-Fab Ab, followed by streptavidin-allophycocyanin, after treatment of Nu-DUL-1 cells (IgG-type) and Daudi cells (IgM-type) with

    Journal: The Journal of Immunology Author Choice

    Article Title: The Bacterial Enzyme IdeS Cleaves the IgG-Type of B Cell Receptor (BCR), Abolishes BCR-Mediated Cell Signaling, and Inhibits Memory B Cell Activation

    doi: 10.4049/jimmunol.1501929

    Figure Lengend Snippet: IdeS cleaves IgG-type, but not IgM-type, of BCR on B cells. ( A ) Flow cytometry analysis of cells stained with biotinylated anti-Fab Ab, followed by streptavidin-allophycocyanin, after treatment of Nu-DUL-1 cells (IgG-type) and Daudi cells (IgM-type) with

    Article Snippet: Cells were stained with a biotinylated Ab specific for the F(ab′)2 portion (cat. no. 109-066-097), followed by streptavidin-allophycocyanin (cat. no. 016-130-084; both from Jackson ImmunoResearch), and the cells were monitored in FL4 using an Accuri C6 flow cytometer.

    Techniques: Flow Cytometry, Cytometry, Staining

    IdeS cleaves IgG-type, but not IgM-type, of BCR on B cells. ( A ) Flow cytometry analysis of cells stained with biotinylated anti-Fab Ab, followed by streptavidin-allophycocyanin, after treatment of Nu-DUL-1 cells (IgG-type) and Daudi cells (IgM-type) with

    Journal: The Journal of Immunology Author Choice

    Article Title: The Bacterial Enzyme IdeS Cleaves the IgG-Type of B Cell Receptor (BCR), Abolishes BCR-Mediated Cell Signaling, and Inhibits Memory B Cell Activation

    doi: 10.4049/jimmunol.1501929

    Figure Lengend Snippet: IdeS cleaves IgG-type, but not IgM-type, of BCR on B cells. ( A ) Flow cytometry analysis of cells stained with biotinylated anti-Fab Ab, followed by streptavidin-allophycocyanin, after treatment of Nu-DUL-1 cells (IgG-type) and Daudi cells (IgM-type) with

    Article Snippet: A total of 10 μg/ml biotinylated anti–CH1-IgG (BAC, cat. no. 7103202100) was used for detection of the F(ab′)2 portion of IgG, and 0.5 μg/ml biotinylated goat anti-human Fc-specific F(ab′)2 fragment (Jackson ImmunoResearch, cat. no. 109-066-098) was used for detection of the Fc portion of IgG.

    Techniques: Flow Cytometry, Cytometry, Staining