biotin sp affinipure f ab 2 fragment goat anti human igg fcγ fragment specific  (Jackson Immuno)

 
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    Name:
    Biotin SP AffiniPure F ab 2 Fragment Goat Anti Human IgG Fcγ Fragment Specific
    Description:
    F ab 2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region F ab 2 fragments have two antigen binding Fab portions linked together by disulfide bonds and therefore they are divalent The average molecular weight is about 110 kDa They are used for specific applications such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G Based on immunoelectrophoresis and or ELISA the antibody reacts with the Fc portion of human IgG heavy chain but not with the Fab portion of human IgG No antibody was detected against human IgM or IgA or against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    Catalog Number:
    109-066-008
    Price:
    129
    Purity:
    The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
    Conjugate:
    Biotin SP long spacer
    Size:
    ml
    Category:
    Secondary Antibody
    Source:
    Goat
    Quantity:
    0 5
    Buy from Supplier


    Structured Review

    Jackson Immuno biotin sp affinipure f ab 2 fragment goat anti human igg fcγ fragment specific
    Biotin SP AffiniPure F ab 2 Fragment Goat Anti Human IgG Fcγ Fragment Specific
    F ab 2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region F ab 2 fragments have two antigen binding Fab portions linked together by disulfide bonds and therefore they are divalent The average molecular weight is about 110 kDa They are used for specific applications such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G Based on immunoelectrophoresis and or ELISA the antibody reacts with the Fc portion of human IgG heavy chain but not with the Fab portion of human IgG No antibody was detected against human IgM or IgA or against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    https://www.bioz.com/result/biotin sp affinipure f ab 2 fragment goat anti human igg fcγ fragment specific/product/Jackson Immuno
    Average 89 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure f ab 2 fragment goat anti human igg fcγ fragment specific - by Bioz Stars, 2020-05
    89/100 stars

    Images

    1) Product Images from "The Bacterial Enzyme IdeS Cleaves the IgG-Type of B Cell Receptor (BCR), Abolishes BCR-Mediated Cell Signaling, and Inhibits Memory B Cell Activation"

    Article Title: The Bacterial Enzyme IdeS Cleaves the IgG-Type of B Cell Receptor (BCR), Abolishes BCR-Mediated Cell Signaling, and Inhibits Memory B Cell Activation

    Journal: The Journal of Immunology Author Choice

    doi: 10.4049/jimmunol.1501929

    IdeS cleaves IgG-type, but not IgM-type, of BCR on B cells. ( A ) Flow cytometry analysis of cells stained with biotinylated anti-Fab Ab, followed by streptavidin-allophycocyanin, after treatment of Nu-DUL-1 cells (IgG-type) and Daudi cells (IgM-type) with
    Figure Legend Snippet: IdeS cleaves IgG-type, but not IgM-type, of BCR on B cells. ( A ) Flow cytometry analysis of cells stained with biotinylated anti-Fab Ab, followed by streptavidin-allophycocyanin, after treatment of Nu-DUL-1 cells (IgG-type) and Daudi cells (IgM-type) with

    Techniques Used: Flow Cytometry, Cytometry, Staining

    2) Product Images from "The Bacterial Enzyme IdeS Cleaves the IgG-Type of B Cell Receptor (BCR), Abolishes BCR-Mediated Cell Signaling, and Inhibits Memory B Cell Activation"

    Article Title: The Bacterial Enzyme IdeS Cleaves the IgG-Type of B Cell Receptor (BCR), Abolishes BCR-Mediated Cell Signaling, and Inhibits Memory B Cell Activation

    Journal: The Journal of Immunology Author Choice

    doi: 10.4049/jimmunol.1501929

    IdeS cleaves IgG-type, but not IgM-type, of BCR on B cells. ( A ) Flow cytometry analysis of cells stained with biotinylated anti-Fab Ab, followed by streptavidin-allophycocyanin, after treatment of Nu-DUL-1 cells (IgG-type) and Daudi cells (IgM-type) with
    Figure Legend Snippet: IdeS cleaves IgG-type, but not IgM-type, of BCR on B cells. ( A ) Flow cytometry analysis of cells stained with biotinylated anti-Fab Ab, followed by streptavidin-allophycocyanin, after treatment of Nu-DUL-1 cells (IgG-type) and Daudi cells (IgM-type) with

    Techniques Used: Flow Cytometry, Cytometry, Staining

    3) Product Images from "The Bacterial Enzyme IdeS Cleaves the IgG-Type of B Cell Receptor (BCR), Abolishes BCR-Mediated Cell Signaling, and Inhibits Memory B Cell Activation"

    Article Title: The Bacterial Enzyme IdeS Cleaves the IgG-Type of B Cell Receptor (BCR), Abolishes BCR-Mediated Cell Signaling, and Inhibits Memory B Cell Activation

    Journal: The Journal of Immunology Author Choice

    doi: 10.4049/jimmunol.1501929

    IdeS cleaves IgG-type, but not IgM-type, of BCR on B cells. ( A ) Flow cytometry analysis of cells stained with biotinylated anti-Fab Ab, followed by streptavidin-allophycocyanin, after treatment of Nu-DUL-1 cells (IgG-type) and Daudi cells (IgM-type) with
    Figure Legend Snippet: IdeS cleaves IgG-type, but not IgM-type, of BCR on B cells. ( A ) Flow cytometry analysis of cells stained with biotinylated anti-Fab Ab, followed by streptavidin-allophycocyanin, after treatment of Nu-DUL-1 cells (IgG-type) and Daudi cells (IgM-type) with

    Techniques Used: Flow Cytometry, Cytometry, Staining

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Complete Removal of Extracellular IgG Antibodies in a Randomized Dose-Escalation Phase I Study with the Bacterial Enzyme IdeS – A Novel Therapeutic Opportunity
    Article Snippet: .. The Fc ELISA used a goat anti-human IgG, Fcγ fragment specific, F(ab’)2 fragment (#109-006-098, Jackson ImmunoResearch Labs Inc.) as catcher antibody and a biotin conjugated goat anti-human IgG, Fcγ fragment specific, F(ab’)2 fragment (# 109-066-098, Jackson ImmunoResearch Labs Inc.) as detector. .. In the Fab ELISA an affinity purified mouse anti-human IgG, F(ab’)2 fragment specific antibody (#209-005-097, Jackson ImmunoResearch Labs Inc.) was used as catcher antibody and biotinylated CaptureSelect IgG-CH1 (#710.3120.100 BAC B.V., Naarden, Netherlands) as detector.

    Staining:

    Article Title: The Bacterial Enzyme IdeS Cleaves the IgG-Type of B Cell Receptor (BCR), Abolishes BCR-Mediated Cell Signaling, and Inhibits Memory B Cell Activation
    Article Snippet: .. Cells were stained with 10 μg/ml biotinylated anti–CH1-IgG (BAC, cat. no. 7103202100) for detection of the F(ab′)2 portion of IgGl; 0.5 μg/ml biotinylated goat anti-human Fc-specific F(ab′)2 fragment (cat. no. 109-066-098, Jackson ImmunoResearch) was used for detection of the Fc portion of IgG. .. Cells were double stained with PE-conjugated anti-CD19 (Immunotools, cat. no. 21270194) and streptavidin-allophycocyanin (Jackson ImmunoResearch, cat. no. 016-130-084).

    Article Title: The Bacterial Enzyme IdeS Cleaves the IgG-Type of B Cell Receptor (BCR), Abolishes BCR-Mediated Cell Signaling, and Inhibits Memory B Cell Activation
    Article Snippet: .. Cells were stained with biotinylated Abs specific for the F(ab′)2 portion (Jackson ImmunoResearch, cat. no. 109-066-097; cross-reacts with L chain present in all Ig subclasses) and the Fc portion of IgG (Jackson ImmunoResearch, cat. no. 109-066-098; specific for IgG H chain) or IgM (GM-80A, ICL). .. Streptavidin-allophycocyanin (Jackson ImmunoResearch, cat. no. 016-130-084) was used to monitor cells in FL4 using an Accuri C6 flow cytometer.

    BAC Assay:

    Article Title: The Bacterial Enzyme IdeS Cleaves the IgG-Type of B Cell Receptor (BCR), Abolishes BCR-Mediated Cell Signaling, and Inhibits Memory B Cell Activation
    Article Snippet: .. Biotinylated anti–CH1-IgG CaptureSelect (BAC, cat. no. 7103202100) was used for detection of the F(ab′)2 portion of IgG, and biotinylated goat anti-human Fc-specific F(ab′)2 fragment (Jackson ImmunoResearch, cat. no. 109-066-098) was used for detection of the Fc portion of IgG. .. Cells were double stained with PE-conjugated anti-CD19 (Exbio, cat. no. IP-305-T100) and streptavidin-allophycocyanin (Jackson ImmunoResearch, cat. no. 016-130-084).

    Article Title: The Bacterial Enzyme IdeS Cleaves the IgG-Type of B Cell Receptor (BCR), Abolishes BCR-Mediated Cell Signaling, and Inhibits Memory B Cell Activation
    Article Snippet: .. Cells were stained with 10 μg/ml biotinylated anti–CH1-IgG (BAC, cat. no. 7103202100) for detection of the F(ab′)2 portion of IgGl; 0.5 μg/ml biotinylated goat anti-human Fc-specific F(ab′)2 fragment (cat. no. 109-066-098, Jackson ImmunoResearch) was used for detection of the Fc portion of IgG. .. Cells were double stained with PE-conjugated anti-CD19 (Immunotools, cat. no. 21270194) and streptavidin-allophycocyanin (Jackson ImmunoResearch, cat. no. 016-130-084).

    Article Title: The Bacterial Enzyme IdeS Cleaves the IgG-Type of B Cell Receptor (BCR), Abolishes BCR-Mediated Cell Signaling, and Inhibits Memory B Cell Activation
    Article Snippet: .. A total of 10 μg/ml biotinylated anti–CH1-IgG (BAC, cat. no. 7103202100) was used for detection of the F(ab′)2 portion of IgG, and 0.5 μg/ml biotinylated goat anti-human Fc-specific F(ab′)2 fragment (Jackson ImmunoResearch, cat. no. 109-066-098) was used for detection of the Fc portion of IgG. .. Cells were double stained with PE-conjugated anti-CD19 (Exbio, cat. no. IP-305-T100) or PE-conjugated anti-CD27 (BD Pharmingen, cat. no. 555441), followed by detection of the biotin-conjugated Abs with streptavidin-allophycocyanin (Jackson ImmunoResearch, cat. no. 016-130-084).

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  • About
  • News
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  • 89
    Jackson Immuno biotin sp affinipure f ab 2 fragment goat anti human igg fcγ fragment specific
    IdeS cleaves IgG-type, but not IgM-type, of BCR on B cells. ( A ) Flow cytometry analysis of cells stained with <t>biotinylated</t> anti-Fab Ab, followed by streptavidin-allophycocyanin, after treatment of Nu-DUL-1 cells (IgG-type) and Daudi cells (IgM-type) with
    Biotin Sp Affinipure F Ab 2 Fragment Goat Anti Human Igg Fcγ Fragment Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 89/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin sp affinipure f ab 2 fragment goat anti human igg fcγ fragment specific/product/Jackson Immuno
    Average 89 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    biotin sp affinipure f ab 2 fragment goat anti human igg fcγ fragment specific - by Bioz Stars, 2020-05
    89/100 stars
      Buy from Supplier

    Image Search Results


    IdeS cleaves IgG-type, but not IgM-type, of BCR on B cells. ( A ) Flow cytometry analysis of cells stained with biotinylated anti-Fab Ab, followed by streptavidin-allophycocyanin, after treatment of Nu-DUL-1 cells (IgG-type) and Daudi cells (IgM-type) with

    Journal: The Journal of Immunology Author Choice

    Article Title: The Bacterial Enzyme IdeS Cleaves the IgG-Type of B Cell Receptor (BCR), Abolishes BCR-Mediated Cell Signaling, and Inhibits Memory B Cell Activation

    doi: 10.4049/jimmunol.1501929

    Figure Lengend Snippet: IdeS cleaves IgG-type, but not IgM-type, of BCR on B cells. ( A ) Flow cytometry analysis of cells stained with biotinylated anti-Fab Ab, followed by streptavidin-allophycocyanin, after treatment of Nu-DUL-1 cells (IgG-type) and Daudi cells (IgM-type) with

    Article Snippet: A total of 10 μg/ml biotinylated anti–CH1-IgG (BAC, cat. no. 7103202100) was used for detection of the F(ab′)2 portion of IgG, and 0.5 μg/ml biotinylated goat anti-human Fc-specific F(ab′)2 fragment (Jackson ImmunoResearch, cat. no. 109-066-098) was used for detection of the Fc portion of IgG.

    Techniques: Flow Cytometry, Cytometry, Staining