biotin goat anti mertk  (Abcam)

 
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    Goat F ab 2 Anti Mouse IgG IgM IgA H L Biotin
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    AB6005
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    Structured Review

    Abcam biotin goat anti mertk
    In vivo knockdown of MAARS inhibits plaque necrosis and increases efferocytosis. a Quantification of plaque necrosis in the lesions of the aortic sinus of LDLR −/− mice fed HCD treated with control ( n = 15) or MAARS gapmers (knockdown (KD), n = 13). b Flow cytometry staining with the efferocytosis marker <t>MerTK</t> in BMDMs treated with or without 20 µM CPT for 2 h and incubated with Jurkat apoptotic cells for 1 h at a BMDM:AC ratio of 1:10. c High resolution confocal images and quantification of MerTK staining in BMDMs treated with 20 µM CPT for 2 h and incubated with Jurkat apoptotic cells (AC) for 1 h at a BMDM:AC ratio of 1:1. d Evaluation of macrophage efferocytosis in vivo by co-staining with MerTK and Mac-3, and quantification of the ratio between double-positive MerTK + /Mac3 + and Mac3 staining alone in the aortic sinus of LDLR −/− mice fed HCD treated with control ( n = 15) or MAARS gapmers (KD, n = 13). e Quantification of the ratio between free TUNEL staining (arrow heads) vs. Mac3-positive TUNEL staining (arrows), reflecting Mac3-positive efferocytotic macrophages, in the aortic sinus lesions LDLR −/− mice fed HCD treated with control ( n = 15) or MAARS gapmeRs (KD, n = 13). f In vitro efferocytosis assay: BMDMs were transfected with control or MAARS gapmeRs in the presence or absence of the indicated control or <t>HuR</t> siRNAs and subsequently incubated with calcein AM-labeled apoptotic Jurkat cells for 1 h. Efferocytosis was quantified as the ratio of calcein-positive (

    https://www.bioz.com/result/biotin goat anti mertk/product/Abcam
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin goat anti mertk - by Bioz Stars, 2021-06
    93/100 stars

    Images

    1) Product Images from "A macrophage-specific lncRNA regulates apoptosis and atherosclerosis by tethering HuR in the nucleus"

    Article Title: A macrophage-specific lncRNA regulates apoptosis and atherosclerosis by tethering HuR in the nucleus

    Journal: Nature Communications

    doi: 10.1038/s41467-020-19664-2

    In vivo knockdown of MAARS inhibits plaque necrosis and increases efferocytosis. a Quantification of plaque necrosis in the lesions of the aortic sinus of LDLR −/− mice fed HCD treated with control ( n = 15) or MAARS gapmers (knockdown (KD), n = 13). b Flow cytometry staining with the efferocytosis marker MerTK in BMDMs treated with or without 20 µM CPT for 2 h and incubated with Jurkat apoptotic cells for 1 h at a BMDM:AC ratio of 1:10. c High resolution confocal images and quantification of MerTK staining in BMDMs treated with 20 µM CPT for 2 h and incubated with Jurkat apoptotic cells (AC) for 1 h at a BMDM:AC ratio of 1:1. d Evaluation of macrophage efferocytosis in vivo by co-staining with MerTK and Mac-3, and quantification of the ratio between double-positive MerTK + /Mac3 + and Mac3 staining alone in the aortic sinus of LDLR −/− mice fed HCD treated with control ( n = 15) or MAARS gapmers (KD, n = 13). e Quantification of the ratio between free TUNEL staining (arrow heads) vs. Mac3-positive TUNEL staining (arrows), reflecting Mac3-positive efferocytotic macrophages, in the aortic sinus lesions LDLR −/− mice fed HCD treated with control ( n = 15) or MAARS gapmeRs (KD, n = 13). f In vitro efferocytosis assay: BMDMs were transfected with control or MAARS gapmeRs in the presence or absence of the indicated control or HuR siRNAs and subsequently incubated with calcein AM-labeled apoptotic Jurkat cells for 1 h. Efferocytosis was quantified as the ratio of calcein-positive (
    Figure Legend Snippet: In vivo knockdown of MAARS inhibits plaque necrosis and increases efferocytosis. a Quantification of plaque necrosis in the lesions of the aortic sinus of LDLR −/− mice fed HCD treated with control ( n = 15) or MAARS gapmers (knockdown (KD), n = 13). b Flow cytometry staining with the efferocytosis marker MerTK in BMDMs treated with or without 20 µM CPT for 2 h and incubated with Jurkat apoptotic cells for 1 h at a BMDM:AC ratio of 1:10. c High resolution confocal images and quantification of MerTK staining in BMDMs treated with 20 µM CPT for 2 h and incubated with Jurkat apoptotic cells (AC) for 1 h at a BMDM:AC ratio of 1:1. d Evaluation of macrophage efferocytosis in vivo by co-staining with MerTK and Mac-3, and quantification of the ratio between double-positive MerTK + /Mac3 + and Mac3 staining alone in the aortic sinus of LDLR −/− mice fed HCD treated with control ( n = 15) or MAARS gapmers (KD, n = 13). e Quantification of the ratio between free TUNEL staining (arrow heads) vs. Mac3-positive TUNEL staining (arrows), reflecting Mac3-positive efferocytotic macrophages, in the aortic sinus lesions LDLR −/− mice fed HCD treated with control ( n = 15) or MAARS gapmeRs (KD, n = 13). f In vitro efferocytosis assay: BMDMs were transfected with control or MAARS gapmeRs in the presence or absence of the indicated control or HuR siRNAs and subsequently incubated with calcein AM-labeled apoptotic Jurkat cells for 1 h. Efferocytosis was quantified as the ratio of calcein-positive (

    Techniques Used: In Vivo, Mouse Assay, Flow Cytometry, Staining, Marker, Incubation, TUNEL Assay, In Vitro, Transfection, Labeling

    Related Articles

    Expressing:

    Article Title: Differential control of metabolic and cardiovascular functions by melanocortin-4 receptors in proopiomelanocortin neurons
    Article Snippet: Sections were placed free-floating in 0.01 M PBS and then incubated in blocking solution (PBS, 0.3% Triton X-100, and 5% normal donkey serum) for 1 h at room temperature. .. The expression of GFP was detected by incubating with primary antibody 1:250 goat anti-GFP biotin (Abcam, Cambridge, MA) diluted in blocking solution for 24 h at 4°C. .. After rinses with PBS (5 times for 5 min), sections were incubated with rabbit anti-Pomc at a dilution of 1:100 for 1 h at room temperature.

    Blocking Assay:

    Article Title: Differential control of metabolic and cardiovascular functions by melanocortin-4 receptors in proopiomelanocortin neurons
    Article Snippet: Sections were placed free-floating in 0.01 M PBS and then incubated in blocking solution (PBS, 0.3% Triton X-100, and 5% normal donkey serum) for 1 h at room temperature. .. The expression of GFP was detected by incubating with primary antibody 1:250 goat anti-GFP biotin (Abcam, Cambridge, MA) diluted in blocking solution for 24 h at 4°C. .. After rinses with PBS (5 times for 5 min), sections were incubated with rabbit anti-Pomc at a dilution of 1:100 for 1 h at room temperature.

    Staining:

    Article Title: A macrophage-specific lncRNA regulates apoptosis and atherosclerosis by tethering HuR in the nucleus
    Article Snippet: Immunofluorescence stainingFor immunofluorescence staining, 6 µm frozen sections of aortic roots and aortic arches were fixed with cold-acetone or 4% paraformaldehyde for 5 and 15 min, respectively, and blocked in PBS containing 3% BSA for 1 h at room temperature. .. Sections were stained with rat anti-Mac2, (Cedarlane, CL8942AP, 1:100), anti-Mac3 (BD Pharmingen, 553322, 1:900), rabbit anti-cleaved caspase-3 (Cell Signaling, 9664, 1:400) rabbit anti-HuR/ELAVL1 (12582S Cell Signaling and AB242410, Abcam), biotin goat anti-MerTK (BAF591, R & D Technologies) in PBS at 4 °C overnight. .. Similarly, BMDMs were plated on microscope slides and after treatment were fixed in 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, and incubated with selected antibodies overnight as above.

    Binding Assay:

    Article Title: Rapid isolation of high affinity human antibodies against the tumor vascular marker Endosialin/TEM1, using a paired yeast display/secretory scFv library platform
    Article Snippet: Yeast-display scFv expression was detected with anti-c-myc mouse monoclonal antibody (mAb), 9E10 (Santa Cruz Biotechnology, Inc., Santa Cruz, CA) and Alexa488 F(ab')2 fragment of goat anti-mouse IgG (H+L) (488 anti-IgG) (Invitrogen, Carlsbad, CA) or PECy5 goat F(ab')2 anti-mouse IgG(H+L) (PE-Cu5 anti-IgG) (Cedarlane Laboratories Limited, Burlington, NC). .. Biotinylated antigen binding to yeast-display scFv was detected with goat anti-biotin-FITC (Abcam, Cambridge, MA) or streptavidin-PE (BD Pharmingen, San Jose, CA). .. ScFv binding to cell lines were detected with APC-conjugated anti-V5 mouse mAb (AbD Serotec, Raleigh, NC) (APC anti-V5) and scFv binding to plastic-immobilized antigen was detected by HRP-conjugated mouse anti-V5 mAb (AbD Serotec) (HRP-anti-V5).

    other:

    Article Title: Keratinocyte Growth Factor (KGF) Modulates Epidermal Progenitor Cell Kinetics through Activation of p63 in Middle Ear Cholesteatoma
    Article Snippet: The secondary antibodies used in this study were HRP-conjugated goat anti-mouse IgG F(ab)’ (Chemicon International #AP124P, 1:100); HRP-conjugated goat anti-rabbit IgG F(ab)’ (Thermo Fisher Scientific #31460, 1:200); FITC-labeled goat anti-biotin (Abcam #ab53469, 1:100); HRP-conjugated goat anti-biotin (Abcam # ab6651, 1:100); Alexa Fluor 546-goat anti-mouse IgG (Thermo Fisher Scientific #A-11030, 1:500); Alexa Fluor 546-goat anti-rabbit IgG (Thermo Fisher Scientific #A-11035, 1:500); and Alexa Fluor 647-goat anti-rabbit IgG (Thermo Fisher Scientific # A-21244, 1:500).

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  • 93
    Abcam biotin goat anti mertk
    In vivo knockdown of MAARS inhibits plaque necrosis and increases efferocytosis. a Quantification of plaque necrosis in the lesions of the aortic sinus of LDLR −/− mice fed HCD treated with control ( n = 15) or MAARS gapmers (knockdown (KD), n = 13). b Flow cytometry staining with the efferocytosis marker <t>MerTK</t> in BMDMs treated with or without 20 µM CPT for 2 h and incubated with Jurkat apoptotic cells for 1 h at a BMDM:AC ratio of 1:10. c High resolution confocal images and quantification of MerTK staining in BMDMs treated with 20 µM CPT for 2 h and incubated with Jurkat apoptotic cells (AC) for 1 h at a BMDM:AC ratio of 1:1. d Evaluation of macrophage efferocytosis in vivo by co-staining with MerTK and Mac-3, and quantification of the ratio between double-positive MerTK + /Mac3 + and Mac3 staining alone in the aortic sinus of LDLR −/− mice fed HCD treated with control ( n = 15) or MAARS gapmers (KD, n = 13). e Quantification of the ratio between free TUNEL staining (arrow heads) vs. Mac3-positive TUNEL staining (arrows), reflecting Mac3-positive efferocytotic macrophages, in the aortic sinus lesions LDLR −/− mice fed HCD treated with control ( n = 15) or MAARS gapmeRs (KD, n = 13). f In vitro efferocytosis assay: BMDMs were transfected with control or MAARS gapmeRs in the presence or absence of the indicated control or <t>HuR</t> siRNAs and subsequently incubated with calcein AM-labeled apoptotic Jurkat cells for 1 h. Efferocytosis was quantified as the ratio of calcein-positive (
    Biotin Goat Anti Mertk, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin goat anti mertk/product/Abcam
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin goat anti mertk - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

    Image Search Results


    In vivo knockdown of MAARS inhibits plaque necrosis and increases efferocytosis. a Quantification of plaque necrosis in the lesions of the aortic sinus of LDLR −/− mice fed HCD treated with control ( n = 15) or MAARS gapmers (knockdown (KD), n = 13). b Flow cytometry staining with the efferocytosis marker MerTK in BMDMs treated with or without 20 µM CPT for 2 h and incubated with Jurkat apoptotic cells for 1 h at a BMDM:AC ratio of 1:10. c High resolution confocal images and quantification of MerTK staining in BMDMs treated with 20 µM CPT for 2 h and incubated with Jurkat apoptotic cells (AC) for 1 h at a BMDM:AC ratio of 1:1. d Evaluation of macrophage efferocytosis in vivo by co-staining with MerTK and Mac-3, and quantification of the ratio between double-positive MerTK + /Mac3 + and Mac3 staining alone in the aortic sinus of LDLR −/− mice fed HCD treated with control ( n = 15) or MAARS gapmers (KD, n = 13). e Quantification of the ratio between free TUNEL staining (arrow heads) vs. Mac3-positive TUNEL staining (arrows), reflecting Mac3-positive efferocytotic macrophages, in the aortic sinus lesions LDLR −/− mice fed HCD treated with control ( n = 15) or MAARS gapmeRs (KD, n = 13). f In vitro efferocytosis assay: BMDMs were transfected with control or MAARS gapmeRs in the presence or absence of the indicated control or HuR siRNAs and subsequently incubated with calcein AM-labeled apoptotic Jurkat cells for 1 h. Efferocytosis was quantified as the ratio of calcein-positive (

    Journal: Nature Communications

    Article Title: A macrophage-specific lncRNA regulates apoptosis and atherosclerosis by tethering HuR in the nucleus

    doi: 10.1038/s41467-020-19664-2

    Figure Lengend Snippet: In vivo knockdown of MAARS inhibits plaque necrosis and increases efferocytosis. a Quantification of plaque necrosis in the lesions of the aortic sinus of LDLR −/− mice fed HCD treated with control ( n = 15) or MAARS gapmers (knockdown (KD), n = 13). b Flow cytometry staining with the efferocytosis marker MerTK in BMDMs treated with or without 20 µM CPT for 2 h and incubated with Jurkat apoptotic cells for 1 h at a BMDM:AC ratio of 1:10. c High resolution confocal images and quantification of MerTK staining in BMDMs treated with 20 µM CPT for 2 h and incubated with Jurkat apoptotic cells (AC) for 1 h at a BMDM:AC ratio of 1:1. d Evaluation of macrophage efferocytosis in vivo by co-staining with MerTK and Mac-3, and quantification of the ratio between double-positive MerTK + /Mac3 + and Mac3 staining alone in the aortic sinus of LDLR −/− mice fed HCD treated with control ( n = 15) or MAARS gapmers (KD, n = 13). e Quantification of the ratio between free TUNEL staining (arrow heads) vs. Mac3-positive TUNEL staining (arrows), reflecting Mac3-positive efferocytotic macrophages, in the aortic sinus lesions LDLR −/− mice fed HCD treated with control ( n = 15) or MAARS gapmeRs (KD, n = 13). f In vitro efferocytosis assay: BMDMs were transfected with control or MAARS gapmeRs in the presence or absence of the indicated control or HuR siRNAs and subsequently incubated with calcein AM-labeled apoptotic Jurkat cells for 1 h. Efferocytosis was quantified as the ratio of calcein-positive (

    Article Snippet: Sections were stained with rat anti-Mac2, (Cedarlane, CL8942AP, 1:100), anti-Mac3 (BD Pharmingen, 553322, 1:900), rabbit anti-cleaved caspase-3 (Cell Signaling, 9664, 1:400) rabbit anti-HuR/ELAVL1 (12582S Cell Signaling and AB242410, Abcam), biotin goat anti-MerTK (BAF591, R & D Technologies) in PBS at 4 °C overnight.

    Techniques: In Vivo, Mouse Assay, Flow Cytometry, Staining, Marker, Incubation, TUNEL Assay, In Vitro, Transfection, Labeling