biotin datp  (Thermo Fisher)


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    Thermo Fisher biotin datp
    Schematic representation of DECAL. ( A ) Construction of Bb-CAL. A ZAP II B. burgdorferi genomic library was screened with radioactively labeled B. burgdorferi rRNA probes and hybridizing clones discarded. The nonribosomal clones were pooled and restriction digested with EcoR V and Sma I. The 200- to 2,000-bp fragments were gel-purified, ligated to PCR adapters, and PCR-amplified. ( B ) Positive selection and amplification. Total RNA isolated from heart and CNS of B. burgdorferi -infected NHPs was reverse transcribed in the presence of <t>biotin-dATP.</t> The <t>biotinylated</t> cDNA samples were hybridized to Bb-CAL under stringent conditions. The cDNA-Bb-CAL hybrids were bound to streptavidin-coated magnetic beads and then washed to remove unhybridized Bb-CAL. ( C ) The bound genomic Bb-CAL (representing the B. burgdorferi transcripts in the CNS/heart) was eluted by boiling and PCR-amplified. The PCR products were radioactively labeled and hybridized to a replicate genomic array.
    Biotin Datp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin datp/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin datp - by Bioz Stars, 2020-01
    93/100 stars

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    1) Product Images from "Borrelia burgdorferi transcriptome in the central nervous system of non-human primates"

    Article Title: Borrelia burgdorferi transcriptome in the central nervous system of non-human primates

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.2432412100

    Schematic representation of DECAL. ( A ) Construction of Bb-CAL. A ZAP II B. burgdorferi genomic library was screened with radioactively labeled B. burgdorferi rRNA probes and hybridizing clones discarded. The nonribosomal clones were pooled and restriction digested with EcoR V and Sma I. The 200- to 2,000-bp fragments were gel-purified, ligated to PCR adapters, and PCR-amplified. ( B ) Positive selection and amplification. Total RNA isolated from heart and CNS of B. burgdorferi -infected NHPs was reverse transcribed in the presence of biotin-dATP. The biotinylated cDNA samples were hybridized to Bb-CAL under stringent conditions. The cDNA-Bb-CAL hybrids were bound to streptavidin-coated magnetic beads and then washed to remove unhybridized Bb-CAL. ( C ) The bound genomic Bb-CAL (representing the B. burgdorferi transcripts in the CNS/heart) was eluted by boiling and PCR-amplified. The PCR products were radioactively labeled and hybridized to a replicate genomic array.
    Figure Legend Snippet: Schematic representation of DECAL. ( A ) Construction of Bb-CAL. A ZAP II B. burgdorferi genomic library was screened with radioactively labeled B. burgdorferi rRNA probes and hybridizing clones discarded. The nonribosomal clones were pooled and restriction digested with EcoR V and Sma I. The 200- to 2,000-bp fragments were gel-purified, ligated to PCR adapters, and PCR-amplified. ( B ) Positive selection and amplification. Total RNA isolated from heart and CNS of B. burgdorferi -infected NHPs was reverse transcribed in the presence of biotin-dATP. The biotinylated cDNA samples were hybridized to Bb-CAL under stringent conditions. The cDNA-Bb-CAL hybrids were bound to streptavidin-coated magnetic beads and then washed to remove unhybridized Bb-CAL. ( C ) The bound genomic Bb-CAL (representing the B. burgdorferi transcripts in the CNS/heart) was eluted by boiling and PCR-amplified. The PCR products were radioactively labeled and hybridized to a replicate genomic array.

    Techniques Used: Labeling, Clone Assay, Purification, Polymerase Chain Reaction, Amplification, Selection, Isolation, Infection, Magnetic Beads

    Related Articles

    Clone Assay:

    Article Title: Nuclear routing networks span between nuclear pore complexes and genomic DNA to guide nucleoplasmic trafficking of biomolecules
    Article Snippet: The coding sequences were cloned into the plasmid vectors and expressed in myelomas (ATCC, Manassas, VA, USA). .. The dNTPs analogs: Biotin-dATP, Biotin-dCTP (Life Technologies, Grand Island, NY), FITC-dUTP, Digoxigenin-dUTP (Hoffmann–La Roche, Basel, Switzerland) were incorporated into the dsDNA for the trafficking studies.

    Amplification:

    Article Title: Towards the understanding of the cocoa transcriptome: Production and analysis of an exhaustive dataset of ESTs of Theobroma cacao L. generated from various tissues and under various conditions
    Article Snippet: According to Glen K Fu (2003) [ ], 3 μl Biotin-dATP (Invitrogen), 3 μl Biotin-dCTP (Invitrogen), 1 μl 5'-NVVVVV-3' primer 30 μM (50 ng), 2 μl 5× First-Strand Buffer, 1 μl BD PowerScript Reverse Transcriptase were added, and the mix was kept at 42°C for 30 min. For capture of the unfinished strand, the reaction was mixed with 600 μl of Streptavidine MagneSphere Paramagnetic Particles (Promega) and eluted as recommended by the supplier. .. A 2 μl aliquot from the first strand synthesis was used for the cDNA Amplification by LD PCR (Clontech).

    Article Title: Nuclear routing networks span between nuclear pore complexes and genomic DNA to guide nucleoplasmic trafficking of biomolecules
    Article Snippet: The coding sequence for the HIV Rev NES – LPPLERLTL was amplified from the HIV Rev plasmid (ATCC, Manassas, VA, USA). .. The dNTPs analogs: Biotin-dATP, Biotin-dCTP (Life Technologies, Grand Island, NY), FITC-dUTP, Digoxigenin-dUTP (Hoffmann–La Roche, Basel, Switzerland) were incorporated into the dsDNA for the trafficking studies.

    Article Title: Borrelia burgdorferi transcriptome in the central nervous system of non-human primates
    Article Snippet: One microgram of total RNA was used to prepare biotinylated cDNA by using biotinylated random hexamer primers, biotin-dATP, and the SuperScript First Strand synthesis system for reverse transcription (Life Technologies, Gaithersburg, MD), essentially as described ( ). .. Positive Selection and Amplification.

    Synthesized:

    Article Title: Towards the understanding of the cocoa transcriptome: Production and analysis of an exhaustive dataset of ESTs of Theobroma cacao L. generated from various tissues and under various conditions
    Article Snippet: Construction of full-length enriched cDNA library First strand cDNA were synthesized using the Clontech BD SMART PCR cDNA Synthesis KIT (cat No 634902) as recommended by the supplier. .. According to Glen K Fu (2003) [ ], 3 μl Biotin-dATP (Invitrogen), 3 μl Biotin-dCTP (Invitrogen), 1 μl 5'-NVVVVV-3' primer 30 μM (50 ng), 2 μl 5× First-Strand Buffer, 1 μl BD PowerScript Reverse Transcriptase were added, and the mix was kept at 42°C for 30 min. For capture of the unfinished strand, the reaction was mixed with 600 μl of Streptavidine MagneSphere Paramagnetic Particles (Promega) and eluted as recommended by the supplier.

    Article Title: Nuclear routing networks span between nuclear pore complexes and genomic DNA to guide nucleoplasmic trafficking of biomolecules
    Article Snippet: The coding sequences for the anti-digoxigenin, anti-biotin, anti-fluorescein Fvs were amplified by polymerase chain reaction using the mix of the plasmid DNA, synthesized primers, dNTPs, and Taq DNA polymerase (Hoffmann–La Roche, Basel, Switzerland) using the Robocycler (Stratagene, San Diego, CA, USA) from the constructs described earlier [ ]. .. The dNTPs analogs: Biotin-dATP, Biotin-dCTP (Life Technologies, Grand Island, NY), FITC-dUTP, Digoxigenin-dUTP (Hoffmann–La Roche, Basel, Switzerland) were incorporated into the dsDNA for the trafficking studies.

    Article Title: HOT1 is a mammalian direct telomere repeat-binding protein contributing to telomerase recruitment
    Article Snippet: .. Telomere pull-down Chemically synthesized oligonucleotides ( ; Metabion) were annealed and polymerized by T4 ligase (Fermentas) and biotinylated with biotin–dATP (Invitrogen) by Klenow fragment (Fermentas) following the manufacturer's instructions. .. Twenty-five microgram baits were immobilized on 750 μg paramagnetic streptavidin beads (Dynabeads MyOne C1, Invitrogen) and subsequently incubated with either 400 μg of SILAC-labelled nuclear extract or 20 μl of supernatants of crude E. coli lysates (3 μg/ml) in PBB buffer (150 mM NaCl, 50 mM Tris–HCl pH 8.0, 10 mM MgCl2 , 0.5% NP-40, Complete Protease Inhibitor without EDTA (Roche)) for 2 h at 4°C on a rotation wheel.

    Construct:

    Article Title: Nuclear routing networks span between nuclear pore complexes and genomic DNA to guide nucleoplasmic trafficking of biomolecules
    Article Snippet: The closed, circular, double stranded DNA (ccdsDNA) plasmid constructs coding for the Fvs targeting catalase, glutathione peroxidase, superoxide dismutase, DNase, and RNase were prepared and tested for their integrity during the trafficking as described earlier [ ]. .. The dNTPs analogs: Biotin-dATP, Biotin-dCTP (Life Technologies, Grand Island, NY), FITC-dUTP, Digoxigenin-dUTP (Hoffmann–La Roche, Basel, Switzerland) were incorporated into the dsDNA for the trafficking studies.

    Incubation:

    Article Title: Towards the understanding of the cocoa transcriptome: Production and analysis of an exhaustive dataset of ESTs of Theobroma cacao L. generated from various tissues and under various conditions
    Article Snippet: Then 2 μl 5× First-Strand Buffer, 1 μl DTT (20 mM); 1 μl dNTP Mix (10 mM of each dNTP), 1 μl BD PowerScript Reverse Transcriptase were added and the mix was incubated at 42°C for 1 hour in an air incubator. .. According to Glen K Fu (2003) [ ], 3 μl Biotin-dATP (Invitrogen), 3 μl Biotin-dCTP (Invitrogen), 1 μl 5'-NVVVVV-3' primer 30 μM (50 ng), 2 μl 5× First-Strand Buffer, 1 μl BD PowerScript Reverse Transcriptase were added, and the mix was kept at 42°C for 30 min. For capture of the unfinished strand, the reaction was mixed with 600 μl of Streptavidine MagneSphere Paramagnetic Particles (Promega) and eluted as recommended by the supplier.

    Article Title: Tet inactivation disrupts YY1 binding and long-range chromatin interactions during embryonic heart development
    Article Snippet: Fill-in master mix containing biotin-dATP (Thermo Fisher Scientific) was added to digest nuclei to generate enzyme-digested overhang and mark the DNA ends with biotin. .. Subsequently, T4 DNA ligase (NEB) was added to the reaction and incubated for 4 h at room temperature to achieve proximity ligation.

    Article Title: HOT1 is a mammalian direct telomere repeat-binding protein contributing to telomerase recruitment
    Article Snippet: Telomere pull-down Chemically synthesized oligonucleotides ( ; Metabion) were annealed and polymerized by T4 ligase (Fermentas) and biotinylated with biotin–dATP (Invitrogen) by Klenow fragment (Fermentas) following the manufacturer's instructions. .. Twenty-five microgram baits were immobilized on 750 μg paramagnetic streptavidin beads (Dynabeads MyOne C1, Invitrogen) and subsequently incubated with either 400 μg of SILAC-labelled nuclear extract or 20 μl of supernatants of crude E. coli lysates (3 μg/ml) in PBB buffer (150 mM NaCl, 50 mM Tris–HCl pH 8.0, 10 mM MgCl2 , 0.5% NP-40, Complete Protease Inhibitor without EDTA (Roche)) for 2 h at 4°C on a rotation wheel.

    Article Title: Histone titration against the genome sets the DNA-to-cytoplasm threshold for the Xenopus midblastula transition
    Article Snippet: pBluescript DNA was cut using EcoRI and BamHI (New England Biolabs) and end-filled using klenow (New England Biolabs) and biotin-dATP (Invitrogen). .. For factor and histone depletion assays, ∼250 ng/µL DNA on beads was incubated in interphase extract at 4 °C for 1–2 h and then removed.

    Article Title: Identification of Iron-Responsive, Differential Gene Expression in the Cyanobacterium Synechocystis sp. Strain PCC 6803 with a Customized Amplification Library
    Article Snippet: One microgram of total RNA was treated with RQ1 RNase-free DNase (Promega) and reverse transcribed with 7.7 μg of biotin-labeled random hexamers and biotin-dATP (one-seventh of total dATP) using Superscript II (GIBCO-BRL) at 45°C for 1 h. Synthesis was terminated by heating the mix at 70°C for 15 min. .. The complementary RNA was removed by the addition of RNase H with incubation for 30 min at 37°C.

    Ligation:

    Article Title: Tet inactivation disrupts YY1 binding and long-range chromatin interactions during embryonic heart development
    Article Snippet: Fill-in master mix containing biotin-dATP (Thermo Fisher Scientific) was added to digest nuclei to generate enzyme-digested overhang and mark the DNA ends with biotin. .. Subsequently, T4 DNA ligase (NEB) was added to the reaction and incubated for 4 h at room temperature to achieve proximity ligation.

    Protease Inhibitor:

    Article Title: Tet inactivation disrupts YY1 binding and long-range chromatin interactions during embryonic heart development
    Article Snippet: Briefly, 1 million crosslinked cardiac cells from mouse embryos or 2 million mouse embryonic stem cells were lysed with Hi-C Lysis Buffer (10 mM Tris–HCl pH 8.0, 10 mM NaCl, 0.2% NP-40 with 1× protease inhibitor cocktail), followed by digestion with 150 U of MboI restriction enzyme (NEB) for 2 h at 37 °C. .. Fill-in master mix containing biotin-dATP (Thermo Fisher Scientific) was added to digest nuclei to generate enzyme-digested overhang and mark the DNA ends with biotin.

    Article Title: HOT1 is a mammalian direct telomere repeat-binding protein contributing to telomerase recruitment
    Article Snippet: Telomere pull-down Chemically synthesized oligonucleotides ( ; Metabion) were annealed and polymerized by T4 ligase (Fermentas) and biotinylated with biotin–dATP (Invitrogen) by Klenow fragment (Fermentas) following the manufacturer's instructions. .. Twenty-five microgram baits were immobilized on 750 μg paramagnetic streptavidin beads (Dynabeads MyOne C1, Invitrogen) and subsequently incubated with either 400 μg of SILAC-labelled nuclear extract or 20 μl of supernatants of crude E. coli lysates (3 μg/ml) in PBB buffer (150 mM NaCl, 50 mM Tris–HCl pH 8.0, 10 mM MgCl2 , 0.5% NP-40, Complete Protease Inhibitor without EDTA (Roche)) for 2 h at 4°C on a rotation wheel.

    Infection:

    Article Title: Borrelia burgdorferi transcriptome in the central nervous system of non-human primates
    Article Snippet: RNAs were isolated from the medulla and heart tissue of B. burgdorferi -infected NHPs (≈2–5 g of each tissue) by using the RNAWIZ RNA isolation reagent (Ambion, Austin, TX) according to the manufacturer's protocol. .. One microgram of total RNA was used to prepare biotinylated cDNA by using biotinylated random hexamer primers, biotin-dATP, and the SuperScript First Strand synthesis system for reverse transcription (Life Technologies, Gaithersburg, MD), essentially as described ( ).

    Polymerase Chain Reaction:

    Article Title: Towards the understanding of the cocoa transcriptome: Production and analysis of an exhaustive dataset of ESTs of Theobroma cacao L. generated from various tissues and under various conditions
    Article Snippet: Construction of full-length enriched cDNA library First strand cDNA were synthesized using the Clontech BD SMART PCR cDNA Synthesis KIT (cat No 634902) as recommended by the supplier. .. According to Glen K Fu (2003) [ ], 3 μl Biotin-dATP (Invitrogen), 3 μl Biotin-dCTP (Invitrogen), 1 μl 5'-NVVVVV-3' primer 30 μM (50 ng), 2 μl 5× First-Strand Buffer, 1 μl BD PowerScript Reverse Transcriptase were added, and the mix was kept at 42°C for 30 min. For capture of the unfinished strand, the reaction was mixed with 600 μl of Streptavidine MagneSphere Paramagnetic Particles (Promega) and eluted as recommended by the supplier.

    Article Title: Nuclear routing networks span between nuclear pore complexes and genomic DNA to guide nucleoplasmic trafficking of biomolecules
    Article Snippet: The coding sequences for the anti-digoxigenin, anti-biotin, anti-fluorescein Fvs were amplified by polymerase chain reaction using the mix of the plasmid DNA, synthesized primers, dNTPs, and Taq DNA polymerase (Hoffmann–La Roche, Basel, Switzerland) using the Robocycler (Stratagene, San Diego, CA, USA) from the constructs described earlier [ ]. .. The dNTPs analogs: Biotin-dATP, Biotin-dCTP (Life Technologies, Grand Island, NY), FITC-dUTP, Digoxigenin-dUTP (Hoffmann–La Roche, Basel, Switzerland) were incorporated into the dsDNA for the trafficking studies.

    Article Title: Borrelia burgdorferi transcriptome in the central nervous system of non-human primates
    Article Snippet: One microgram of total RNA was used to prepare biotinylated cDNA by using biotinylated random hexamer primers, biotin-dATP, and the SuperScript First Strand synthesis system for reverse transcription (Life Technologies, Gaithersburg, MD), essentially as described ( ). .. The Bb-CAL bound to the beads was eluted by boiling and PCR-amplified by using Uniamp primers, as described , and schematically outlined in .

    Binding Assay:

    Article Title: Nuclear routing networks span between nuclear pore complexes and genomic DNA to guide nucleoplasmic trafficking of biomolecules
    Article Snippet: The dNTPs analogs: Biotin-dATP, Biotin-dCTP (Life Technologies, Grand Island, NY), FITC-dUTP, Digoxigenin-dUTP (Hoffmann–La Roche, Basel, Switzerland) were incorporated into the dsDNA for the trafficking studies. .. The Fvs were expressed to contain either directly coordinating domains for Gd, Eu, or Tb, or binding groups for DTPA or DOTA.

    Hi-C:

    Article Title: Tet inactivation disrupts YY1 binding and long-range chromatin interactions during embryonic heart development
    Article Snippet: Briefly, 1 million crosslinked cardiac cells from mouse embryos or 2 million mouse embryonic stem cells were lysed with Hi-C Lysis Buffer (10 mM Tris–HCl pH 8.0, 10 mM NaCl, 0.2% NP-40 with 1× protease inhibitor cocktail), followed by digestion with 150 U of MboI restriction enzyme (NEB) for 2 h at 37 °C. .. Fill-in master mix containing biotin-dATP (Thermo Fisher Scientific) was added to digest nuclei to generate enzyme-digested overhang and mark the DNA ends with biotin.

    Magnetic Beads:

    Article Title: Borrelia burgdorferi transcriptome in the central nervous system of non-human primates
    Article Snippet: One microgram of total RNA was used to prepare biotinylated cDNA by using biotinylated random hexamer primers, biotin-dATP, and the SuperScript First Strand synthesis system for reverse transcription (Life Technologies, Gaithersburg, MD), essentially as described ( ). .. Equal amounts of biotinylated cDNA prepared from medulla and heart (normalized based on the levels of flaB transcripts) were hybridized separately to Bb-CAL and the biotinylated cDNA-Bb-CAL hybrids bound to streptavidin-coated magnetic beads (Dynal, Lake Success, NY) as shown in .

    Isolation:

    Article Title: Nuclear routing networks span between nuclear pore complexes and genomic DNA to guide nucleoplasmic trafficking of biomolecules
    Article Snippet: The dNTPs analogs: Biotin-dATP, Biotin-dCTP (Life Technologies, Grand Island, NY), FITC-dUTP, Digoxigenin-dUTP (Hoffmann–La Roche, Basel, Switzerland) were incorporated into the dsDNA for the trafficking studies. .. The genomic DNA was isolated (Life Technologies, Foster City, CA, USA) and sheared using Branson ultrasound generator (Emerson, Danbury, CT, USA).

    Article Title: Borrelia burgdorferi transcriptome in the central nervous system of non-human primates
    Article Snippet: RNAs were isolated from the medulla and heart tissue of B. burgdorferi -infected NHPs (≈2–5 g of each tissue) by using the RNAWIZ RNA isolation reagent (Ambion, Austin, TX) according to the manufacturer's protocol. .. One microgram of total RNA was used to prepare biotinylated cDNA by using biotinylated random hexamer primers, biotin-dATP, and the SuperScript First Strand synthesis system for reverse transcription (Life Technologies, Gaithersburg, MD), essentially as described ( ).

    Size-exclusion Chromatography:

    Article Title: Towards the understanding of the cocoa transcriptome: Production and analysis of an exhaustive dataset of ESTs of Theobroma cacao L. generated from various tissues and under various conditions
    Article Snippet: According to Glen K Fu (2003) [ ], 3 μl Biotin-dATP (Invitrogen), 3 μl Biotin-dCTP (Invitrogen), 1 μl 5'-NVVVVV-3' primer 30 μM (50 ng), 2 μl 5× First-Strand Buffer, 1 μl BD PowerScript Reverse Transcriptase were added, and the mix was kept at 42°C for 30 min. For capture of the unfinished strand, the reaction was mixed with 600 μl of Streptavidine MagneSphere Paramagnetic Particles (Promega) and eluted as recommended by the supplier. .. The PCR reaction consisted of 18 to 25 PCR cycles at 95°C for 15 sec, 65°C for 30 sec, 68°C for 6 min, following with a final extension at 70°c for 10 min. After comparison of fragment sizes with those of model species (rice and Arabidopsis ), fragment sizes of some cDNA libraries were improved using cDNA size fractionation.

    Sequencing:

    Article Title: Nuclear routing networks span between nuclear pore complexes and genomic DNA to guide nucleoplasmic trafficking of biomolecules
    Article Snippet: The coding sequence for the HIV Rev NES – LPPLERLTL was amplified from the HIV Rev plasmid (ATCC, Manassas, VA, USA). .. The dNTPs analogs: Biotin-dATP, Biotin-dCTP (Life Technologies, Grand Island, NY), FITC-dUTP, Digoxigenin-dUTP (Hoffmann–La Roche, Basel, Switzerland) were incorporated into the dsDNA for the trafficking studies.

    HiChIP:

    Article Title: Tet inactivation disrupts YY1 binding and long-range chromatin interactions during embryonic heart development
    Article Snippet: Paragraph title: HiChIP library construction and data analysis ... Fill-in master mix containing biotin-dATP (Thermo Fisher Scientific) was added to digest nuclei to generate enzyme-digested overhang and mark the DNA ends with biotin.

    cDNA Library Assay:

    Article Title: Towards the understanding of the cocoa transcriptome: Production and analysis of an exhaustive dataset of ESTs of Theobroma cacao L. generated from various tissues and under various conditions
    Article Snippet: Paragraph title: Construction of full-length enriched cDNA library ... According to Glen K Fu (2003) [ ], 3 μl Biotin-dATP (Invitrogen), 3 μl Biotin-dCTP (Invitrogen), 1 μl 5'-NVVVVV-3' primer 30 μM (50 ng), 2 μl 5× First-Strand Buffer, 1 μl BD PowerScript Reverse Transcriptase were added, and the mix was kept at 42°C for 30 min. For capture of the unfinished strand, the reaction was mixed with 600 μl of Streptavidine MagneSphere Paramagnetic Particles (Promega) and eluted as recommended by the supplier.

    Plasmid Preparation:

    Article Title: Nuclear routing networks span between nuclear pore complexes and genomic DNA to guide nucleoplasmic trafficking of biomolecules
    Article Snippet: The closed, circular, double stranded DNA (ccdsDNA) plasmid constructs coding for the Fvs targeting catalase, glutathione peroxidase, superoxide dismutase, DNase, and RNase were prepared and tested for their integrity during the trafficking as described earlier [ ]. .. The dNTPs analogs: Biotin-dATP, Biotin-dCTP (Life Technologies, Grand Island, NY), FITC-dUTP, Digoxigenin-dUTP (Hoffmann–La Roche, Basel, Switzerland) were incorporated into the dsDNA for the trafficking studies.

    Selection:

    Article Title: Borrelia burgdorferi transcriptome in the central nervous system of non-human primates
    Article Snippet: One microgram of total RNA was used to prepare biotinylated cDNA by using biotinylated random hexamer primers, biotin-dATP, and the SuperScript First Strand synthesis system for reverse transcription (Life Technologies, Gaithersburg, MD), essentially as described ( ). .. Positive Selection and Amplification.

    Random Hexamer Labeling:

    Article Title: Borrelia burgdorferi transcriptome in the central nervous system of non-human primates
    Article Snippet: .. One microgram of total RNA was used to prepare biotinylated cDNA by using biotinylated random hexamer primers, biotin-dATP, and the SuperScript First Strand synthesis system for reverse transcription (Life Technologies, Gaithersburg, MD), essentially as described ( ). .. Positive Selection and Amplification.

    Fractionation:

    Article Title: Towards the understanding of the cocoa transcriptome: Production and analysis of an exhaustive dataset of ESTs of Theobroma cacao L. generated from various tissues and under various conditions
    Article Snippet: According to Glen K Fu (2003) [ ], 3 μl Biotin-dATP (Invitrogen), 3 μl Biotin-dCTP (Invitrogen), 1 μl 5'-NVVVVV-3' primer 30 μM (50 ng), 2 μl 5× First-Strand Buffer, 1 μl BD PowerScript Reverse Transcriptase were added, and the mix was kept at 42°C for 30 min. For capture of the unfinished strand, the reaction was mixed with 600 μl of Streptavidine MagneSphere Paramagnetic Particles (Promega) and eluted as recommended by the supplier. .. The PCR reaction consisted of 18 to 25 PCR cycles at 95°C for 15 sec, 65°C for 30 sec, 68°C for 6 min, following with a final extension at 70°c for 10 min. After comparison of fragment sizes with those of model species (rice and Arabidopsis ), fragment sizes of some cDNA libraries were improved using cDNA size fractionation.

    Lysis:

    Article Title: Tet inactivation disrupts YY1 binding and long-range chromatin interactions during embryonic heart development
    Article Snippet: Briefly, 1 million crosslinked cardiac cells from mouse embryos or 2 million mouse embryonic stem cells were lysed with Hi-C Lysis Buffer (10 mM Tris–HCl pH 8.0, 10 mM NaCl, 0.2% NP-40 with 1× protease inhibitor cocktail), followed by digestion with 150 U of MboI restriction enzyme (NEB) for 2 h at 37 °C. .. Fill-in master mix containing biotin-dATP (Thermo Fisher Scientific) was added to digest nuclei to generate enzyme-digested overhang and mark the DNA ends with biotin.

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    Thermo Fisher biotin 14 datp
    Biotin 14 Datp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin 14 datp/product/Thermo Fisher
    Average 90 stars, based on 46 article reviews
    Price from $9.99 to $1999.99
    biotin 14 datp - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

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