biotin datp  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 89

    Structured Review

    Thermo Fisher biotin datp
    Schematic representation of DECAL. ( A ) Construction of Bb-CAL. A ZAP II B. burgdorferi genomic library was screened with radioactively labeled B. burgdorferi rRNA probes and hybridizing clones discarded. The nonribosomal clones were pooled and restriction digested with EcoR V and Sma I. The 200- to 2,000-bp fragments were gel-purified, ligated to PCR adapters, and PCR-amplified. ( B ) Positive selection and amplification. Total RNA isolated from heart and CNS of B. burgdorferi -infected NHPs was reverse transcribed in the presence of <t>biotin-dATP.</t> The <t>biotinylated</t> cDNA samples were hybridized to Bb-CAL under stringent conditions. The cDNA-Bb-CAL hybrids were bound to streptavidin-coated magnetic beads and then washed to remove unhybridized Bb-CAL. ( C ) The bound genomic Bb-CAL (representing the B. burgdorferi transcripts in the CNS/heart) was eluted by boiling and PCR-amplified. The PCR products were radioactively labeled and hybridized to a replicate genomic array.
    Biotin Datp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin datp/product/Thermo Fisher
    Average 89 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    biotin datp - by Bioz Stars, 2020-08
    89/100 stars

    Images

    1) Product Images from "Borrelia burgdorferi transcriptome in the central nervous system of non-human primates"

    Article Title: Borrelia burgdorferi transcriptome in the central nervous system of non-human primates

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.2432412100

    Schematic representation of DECAL. ( A ) Construction of Bb-CAL. A ZAP II B. burgdorferi genomic library was screened with radioactively labeled B. burgdorferi rRNA probes and hybridizing clones discarded. The nonribosomal clones were pooled and restriction digested with EcoR V and Sma I. The 200- to 2,000-bp fragments were gel-purified, ligated to PCR adapters, and PCR-amplified. ( B ) Positive selection and amplification. Total RNA isolated from heart and CNS of B. burgdorferi -infected NHPs was reverse transcribed in the presence of biotin-dATP. The biotinylated cDNA samples were hybridized to Bb-CAL under stringent conditions. The cDNA-Bb-CAL hybrids were bound to streptavidin-coated magnetic beads and then washed to remove unhybridized Bb-CAL. ( C ) The bound genomic Bb-CAL (representing the B. burgdorferi transcripts in the CNS/heart) was eluted by boiling and PCR-amplified. The PCR products were radioactively labeled and hybridized to a replicate genomic array.
    Figure Legend Snippet: Schematic representation of DECAL. ( A ) Construction of Bb-CAL. A ZAP II B. burgdorferi genomic library was screened with radioactively labeled B. burgdorferi rRNA probes and hybridizing clones discarded. The nonribosomal clones were pooled and restriction digested with EcoR V and Sma I. The 200- to 2,000-bp fragments were gel-purified, ligated to PCR adapters, and PCR-amplified. ( B ) Positive selection and amplification. Total RNA isolated from heart and CNS of B. burgdorferi -infected NHPs was reverse transcribed in the presence of biotin-dATP. The biotinylated cDNA samples were hybridized to Bb-CAL under stringent conditions. The cDNA-Bb-CAL hybrids were bound to streptavidin-coated magnetic beads and then washed to remove unhybridized Bb-CAL. ( C ) The bound genomic Bb-CAL (representing the B. burgdorferi transcripts in the CNS/heart) was eluted by boiling and PCR-amplified. The PCR products were radioactively labeled and hybridized to a replicate genomic array.

    Techniques Used: Labeling, Clone Assay, Purification, Polymerase Chain Reaction, Amplification, Selection, Isolation, Infection, Magnetic Beads

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Examination of the Borrelia burgdorferi Transcriptome in Ixodes scapularis during Feeding
    Article Snippet: .. The total RNA (∼1 μg) isolated from unfed and fed nymphs was reverse transcribed in the presence of biotin-labeled random hexamers and biotin dATP by use of the Superscript first-strand synthesis system for reverse transcription-PCR (Life Technologies, Inc., Gaithersburg, Md.) at 50°C for 1 h. Aliquots of the resultant cDNA pools were tested by PCR with B. burgdorferi flaB primers ( ). ..

    Isolation:

    Article Title: Examination of the Borrelia burgdorferi Transcriptome in Ixodes scapularis during Feeding
    Article Snippet: .. The total RNA (∼1 μg) isolated from unfed and fed nymphs was reverse transcribed in the presence of biotin-labeled random hexamers and biotin dATP by use of the Superscript first-strand synthesis system for reverse transcription-PCR (Life Technologies, Inc., Gaithersburg, Md.) at 50°C for 1 h. Aliquots of the resultant cDNA pools were tested by PCR with B. burgdorferi flaB primers ( ). ..

    Random Hexamer Labeling:

    Article Title: Borrelia burgdorferi transcriptome in the central nervous system of non-human primates
    Article Snippet: .. One microgram of total RNA was used to prepare biotinylated cDNA by using biotinylated random hexamer primers, biotin-dATP, and the SuperScript First Strand synthesis system for reverse transcription (Life Technologies, Gaithersburg, MD), essentially as described ( ). .. Positive Selection and Amplification.

    Nick Translation:

    Article Title: Cytogenetic characterization of F1, F2 and backcross hybrids of the Neotropical catfish species Pseudoplatystoma corruscans and Pseudoplatystoma reticulatum (Pimelodidae, Siluriformes)
    Article Snippet: .. The probes were labelled with biotin-dATP (18S) and digoxi-genin-dUTP (5S) by nick translation, according to the manufacturer’s instructions (Bionick labelling system, Gibco BRL). .. The slides were counterstained with 4′ 6-diamidino-2-phenylindole (DAPI) and the metaphases then observed and photographed with an Olympus BX50 epi-fluorescence photo-microscope.

    Plasmid Preparation:

    Article Title: VCP/p97 extracts sterically trapped Ku70/80 rings from DNA in double strand break repair
    Article Snippet: .. Briefly, pBluescript SK+ vector was cut and filled with Klenow (New England Biolabs), biotin-dATP and biotin-dUTP (Chemcyte) and bound to M280 dynabeads (Invitrogen). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98
    Thermo Fisher biotin 14 datp
    Schematic illustration of the principle of antarctic thermal-sensitive uracil-DNA-glycosylase-supplemented polymerase spiral reaction (ATSU-PSR) technique for eliminating carryover contamination. Two steps are needed for ATSU-PSR technique for removing carryover contamination. During the first stage, all PSR complicons labeled with dUTP in the presence of Bst 2.0 enzyme and dUTP. During the second stage, all subsequent ATSU-PSR amplifications are digested using ATSU, which specifically cleave carryover contaminants by removing uracil in amplicons from previous reactions. Importantly, ATSU is heat inactivated during the PSR amplification stage (63°C), and the digested contaminants are degraded, ensuring that only the target templates are amplified. In addition, three components, including fluorescein isothiocyanate (FITC)-labeled primer, <t>biotin-14-dCTP,</t> and <t>biotin-14-dATP,</t> are added into ATSU-PSR mixtures for forming the biotin- and FITC-attached duplex products.
    Biotin 14 Datp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin 14 datp/product/Thermo Fisher
    Average 98 stars, based on 76 article reviews
    Price from $9.99 to $1999.99
    biotin 14 datp - by Bioz Stars, 2020-08
    98/100 stars
      Buy from Supplier

    Image Search Results


    Schematic illustration of the principle of antarctic thermal-sensitive uracil-DNA-glycosylase-supplemented polymerase spiral reaction (ATSU-PSR) technique for eliminating carryover contamination. Two steps are needed for ATSU-PSR technique for removing carryover contamination. During the first stage, all PSR complicons labeled with dUTP in the presence of Bst 2.0 enzyme and dUTP. During the second stage, all subsequent ATSU-PSR amplifications are digested using ATSU, which specifically cleave carryover contaminants by removing uracil in amplicons from previous reactions. Importantly, ATSU is heat inactivated during the PSR amplification stage (63°C), and the digested contaminants are degraded, ensuring that only the target templates are amplified. In addition, three components, including fluorescein isothiocyanate (FITC)-labeled primer, biotin-14-dCTP, and biotin-14-dATP, are added into ATSU-PSR mixtures for forming the biotin- and FITC-attached duplex products.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Simultaneous Nucleic Acids Detection and Elimination of Carryover Contamination With Nanoparticles-Based Biosensor- and Antarctic Thermal Sensitive Uracil-DNA-Glycosylase-Supplemented Polymerase Spiral Reaction

    doi: 10.3389/fbioe.2019.00401

    Figure Lengend Snippet: Schematic illustration of the principle of antarctic thermal-sensitive uracil-DNA-glycosylase-supplemented polymerase spiral reaction (ATSU-PSR) technique for eliminating carryover contamination. Two steps are needed for ATSU-PSR technique for removing carryover contamination. During the first stage, all PSR complicons labeled with dUTP in the presence of Bst 2.0 enzyme and dUTP. During the second stage, all subsequent ATSU-PSR amplifications are digested using ATSU, which specifically cleave carryover contaminants by removing uracil in amplicons from previous reactions. Importantly, ATSU is heat inactivated during the PSR amplification stage (63°C), and the digested contaminants are degraded, ensuring that only the target templates are amplified. In addition, three components, including fluorescein isothiocyanate (FITC)-labeled primer, biotin-14-dCTP, and biotin-14-dATP, are added into ATSU-PSR mixtures for forming the biotin- and FITC-attached duplex products.

    Article Snippet: Biotin-14-dCTP and biotin-14-dATP were obtained from Thermo Scientific Co., Ltd. (Shanghai, China).

    Techniques: Labeling, Amplification

    Outline of nanoparticle-based biosensor-supplemented polymerase spiral reaction assay (NB-PSR). (A) Outline of PSR with Ft* primer, biotin-14-dCTP, and biotin-14-dATP. (B) The detailed structure of NB. (C) The schematic illustration of the principle of NB for visualization of PSR products. (D) Interpretation of the results: (I) positive (two red bands, including test line and control line, appeared on the visual region of NB); (II) negative (only the control line region showed a red band).

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Simultaneous Nucleic Acids Detection and Elimination of Carryover Contamination With Nanoparticles-Based Biosensor- and Antarctic Thermal Sensitive Uracil-DNA-Glycosylase-Supplemented Polymerase Spiral Reaction

    doi: 10.3389/fbioe.2019.00401

    Figure Lengend Snippet: Outline of nanoparticle-based biosensor-supplemented polymerase spiral reaction assay (NB-PSR). (A) Outline of PSR with Ft* primer, biotin-14-dCTP, and biotin-14-dATP. (B) The detailed structure of NB. (C) The schematic illustration of the principle of NB for visualization of PSR products. (D) Interpretation of the results: (I) positive (two red bands, including test line and control line, appeared on the visual region of NB); (II) negative (only the control line region showed a red band).

    Article Snippet: Biotin-14-dCTP and biotin-14-dATP were obtained from Thermo Scientific Co., Ltd. (Shanghai, China).

    Techniques: