biotin conjugated rabbit anti human igg  (Thermo Fisher)


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  • 91
    Name:
    Rabbit anti Human IgG Biotin
    Description:

    Catalog Number:
    PA1-28586
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    None
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    Structured Review

    Thermo Fisher biotin conjugated rabbit anti human igg

    https://www.bioz.com/result/biotin conjugated rabbit anti human igg/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin conjugated rabbit anti human igg - by Bioz Stars, 2021-06
    91/100 stars

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    Incubation:

    Article Title: V1V2-specific complement activating serum IgG as a correlate of reduced HIV-1 infection risk in RV144
    Article Snippet: .. After 1 hour incubation at room temperature, beads were washed with Bioplex buffer and incubated with 100 μl of a 1:2,500 dilution of biotin-conjugated rabbit anti-human IgG (heavy and light chain specific, Thermo Scientific, Cat. No. OK1781367) for 30 minutes at room temperature. .. The beads were washed with Bioplex buffer and incubated at room temperature with 100 μl of a 1:500 dilution of PE-Streptavidin (BD Pharmingen, Cat. No. 554061) for 30 minutes.

    Recombinant:

    Article Title: An Adhesion Based Approach for the Detection of Esophageal Cancer
    Article Snippet: To investigate the calcium dependence of E-selectin binding, recombinant E-selectin-IgG chimeras were separately diluted in two different buffers; chimera buffer [1% bovine serum albumin (BSA) in DPBS with Ca2+ or Mg2+ ] and negative control buffer containing EDTA [1% BSA and 20 mM EDTA in DPBS without Ca2+ or Mg2+ ]. .. Suspensions of OE19 and HEsEpiC cells were treated with recombinant murine E-selectin IgG1 chimera (rmE-Sel), recombinant human E-selectin IgG1 chimera (rhE-Sel) or human IgG1 (hIgG) (Sigma-Aldrich), followed by a biotinylated rabbit anti-human IgG Fc polyclonal secondary antibody (Thermo Fisher Scientific, Waltham, MA) and finally phycoerythrin (PE) conjugated streptavidin (SA) (BD Biosciences). .. Each treatment was for 30 minutes on ice and followed by washes in chimera or negative control buffers.

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  • 93
    Thermo Fisher proliferation marker ki67 rabbit antibody
    Amiodarone enhanced liver growth and hepatocyte proliferation in the liver regeneration after PHx. Wild-type mice were intraperitoneally injected with vehicle (Veh), amiodarone (AD), or chloroquine (CQ) at 0.5 h before PHx or sham operation and then once per day until 168 h. Liver tissues were harvested at 0–168 h after surgery. Liver-to-body-weight ratios were calculated ( A ). Liver sections at 24 h after the sham operation, PHx with Veh, AD, or CQ were stained with H E; original magnification, 400X ( B ). Representative immunohistochemical staining of <t>Ki67</t> is shown ( C ). The percentage of Ki67-positive nuclei in hepatocyte was counted under low-power fields (200 ×) in 15 random sections from at least six different mice ( D ). Liver tissues were harvested at 0 h, 12 h, 24 h, or 48 h after surgery, and the tissue extracts were analyzed for PCNA, cyclin A, B, D1, E, p21, TGF-β1, and β-actin protein by Western blotting ( E,F ). The values are shown as the mean ± SD in the bar graph and compared by Student’s t test. # P
    Proliferation Marker Ki67 Rabbit Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/proliferation marker ki67 rabbit antibody/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    proliferation marker ki67 rabbit antibody - by Bioz Stars, 2021-06
    93/100 stars
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    95
    Thermo Fisher mouse anti rabbit biotin conjugated antibody
    Demonstration of expression of the goat uromodulin – GFP transgene and endogenous uromodulin in kidney sections by immunostaining. ( A ). H E kidney TAL region staining of a control non-transgenic <t>mouse.</t> The arrows show microstructures with thick walls indicative of the thick segment of the ascending limb of Henle's loop within the TAL region. Fixed kidney sections from a negative control mouse ( B ) and the 99-122-1-A5M transgenic mouse ( C ) were stained with a polyclonal <t>anti-human</t> uromodulin antibody, followed by treatment with Texas-Red <t>conjugated</t> secondary anti <t>rabbit</t> IgG antibody. Expression of endogenous uromodulin in the cells (red) appears in a punctuated pattern, whereas the GFP expression is cytoplasmic (green). Immunostaining of similar sections for uromodulin confirmed that expression is restricted to tubular epithelial cells of these structures ( B, C ) indicated by red. The same sections observed for GFP expression by confocal microscopy indicated that expression was restricted to similar structures as in B C but not co-expressed with the endogenous uromodulin staining. As expected GFP was absent in the renal sections obtained from a non-transgenic mouse. Both GFP ( D, F ) (green) and uromodulin ( E, F ) (red) were co-expressed in cells in the TAL segment (transgenic mouse, 99-122-1-A5M).
    Mouse Anti Rabbit Biotin Conjugated Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti rabbit biotin conjugated antibody/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti rabbit biotin conjugated antibody - by Bioz Stars, 2021-06
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    90
    Thermo Fisher biotin conjugated foxp3 mab
    PGN induced inhibition of <t>Foxp3</t> expression is independent of IL-6, IL-6R, STAT1, STAT3, STAT4 and STAT6
    Biotin Conjugated Foxp3 Mab, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin conjugated foxp3 mab/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    93
    Thermo Fisher biotin conjugated goat anti rabbit igg
    Proximal tubule cells secrete <t>pro-AOAH.</t> (A) Diagram of AOAH structure, showing the conversion of the precursor (pro-AOAH) into mature AOAH. (B) Production of 35 S-AOAH by porcine proximal tubule cells in vitro. Labeled AOAH was immunoprecipitated as described in Methods and studied by SDS-PAGE and autoradiography. M, medium; L, lysate. Antibody +, anti-AOAH; antibody −, preimmune <t>IgG.</t> Only pro-AOAH is seen in the media, whereas the lysate contains both pro-AOAH and mature AOAH.
    Biotin Conjugated Goat Anti Rabbit Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin conjugated goat anti rabbit igg/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin conjugated goat anti rabbit igg - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

    Image Search Results


    Amiodarone enhanced liver growth and hepatocyte proliferation in the liver regeneration after PHx. Wild-type mice were intraperitoneally injected with vehicle (Veh), amiodarone (AD), or chloroquine (CQ) at 0.5 h before PHx or sham operation and then once per day until 168 h. Liver tissues were harvested at 0–168 h after surgery. Liver-to-body-weight ratios were calculated ( A ). Liver sections at 24 h after the sham operation, PHx with Veh, AD, or CQ were stained with H E; original magnification, 400X ( B ). Representative immunohistochemical staining of Ki67 is shown ( C ). The percentage of Ki67-positive nuclei in hepatocyte was counted under low-power fields (200 ×) in 15 random sections from at least six different mice ( D ). Liver tissues were harvested at 0 h, 12 h, 24 h, or 48 h after surgery, and the tissue extracts were analyzed for PCNA, cyclin A, B, D1, E, p21, TGF-β1, and β-actin protein by Western blotting ( E,F ). The values are shown as the mean ± SD in the bar graph and compared by Student’s t test. # P

    Journal: Scientific Reports

    Article Title: Amiodarone as an autophagy promoter reduces liver injury and enhances liver regeneration and survival in mice after partial hepatectomy

    doi: 10.1038/srep15807

    Figure Lengend Snippet: Amiodarone enhanced liver growth and hepatocyte proliferation in the liver regeneration after PHx. Wild-type mice were intraperitoneally injected with vehicle (Veh), amiodarone (AD), or chloroquine (CQ) at 0.5 h before PHx or sham operation and then once per day until 168 h. Liver tissues were harvested at 0–168 h after surgery. Liver-to-body-weight ratios were calculated ( A ). Liver sections at 24 h after the sham operation, PHx with Veh, AD, or CQ were stained with H E; original magnification, 400X ( B ). Representative immunohistochemical staining of Ki67 is shown ( C ). The percentage of Ki67-positive nuclei in hepatocyte was counted under low-power fields (200 ×) in 15 random sections from at least six different mice ( D ). Liver tissues were harvested at 0 h, 12 h, 24 h, or 48 h after surgery, and the tissue extracts were analyzed for PCNA, cyclin A, B, D1, E, p21, TGF-β1, and β-actin protein by Western blotting ( E,F ). The values are shown as the mean ± SD in the bar graph and compared by Student’s t test. # P

    Article Snippet: Immunohistochemistry Liver paraffin sections from each sample were cut into 5-μm sections and stained with proliferation marker Ki67 rabbit antibody (Thermo Fisher Scientific, PA5-19462, Waltham, MA, USA) using the avidin-biotin-peroxidase complex technique (Vectastain ABC kit and DAB peroxidase substrate kit, Vector Laboratories, Burlingame, CA, USA).

    Techniques: Mouse Assay, Injection, Staining, Immunohistochemistry, Western Blot

    Inhibition of autophagy reduced liver growth and hepatocyte proliferation in the early phase of liver regeneration after PHx. Wild-type mice were given control or Atg7-specific siRNA for 48 h before treatment with PHx. Liver tissues were harvested at 24 h after surgery and tissue extracts were analyzed for Atg7, LC3-II, and β-actin by Western blotting ( A ). The liver-to-body-weight ratios were calculated ( B ). Representative immunohistochemical staining of Ki67 is shown. Scale bar, 50 μm ( C ). The percentage of Ki67-positive nuclei in hepatocytes was counted in low-power field (200X) in 15 random sections from 3 different mice ( D ). Tissue extracts were analyzed for PCNA, cyclin D1, TGF-β1, and β-actin by Western blotting ( E ). Immunohistochemical staining of senescence-associated β-galactosidase (SA-β-gal) in hepatocytes. Scale bar, 100 μm ( F ). Fold-changes in IL-6 ( G ) and IL-8 ( H ) mRNA expression at 24 h after 70% PHx. Wild-type mice were intraperitoneally injected with vehicle (Veh) or chloroquine (CQ) at 0.5 h before PHx or the sham operation and then once per day until 48 h. Liver tissues were harvested at 0–48 h after surgery and tissue extracts were analyzed for PCNA, cyclin D1, TGF-β1, and β-actin by Western blotting ( I,J ). The values are shown as the mean ± SD in the bar graph and compared using Student’s t test. #P

    Journal: Scientific Reports

    Article Title: Amiodarone as an autophagy promoter reduces liver injury and enhances liver regeneration and survival in mice after partial hepatectomy

    doi: 10.1038/srep15807

    Figure Lengend Snippet: Inhibition of autophagy reduced liver growth and hepatocyte proliferation in the early phase of liver regeneration after PHx. Wild-type mice were given control or Atg7-specific siRNA for 48 h before treatment with PHx. Liver tissues were harvested at 24 h after surgery and tissue extracts were analyzed for Atg7, LC3-II, and β-actin by Western blotting ( A ). The liver-to-body-weight ratios were calculated ( B ). Representative immunohistochemical staining of Ki67 is shown. Scale bar, 50 μm ( C ). The percentage of Ki67-positive nuclei in hepatocytes was counted in low-power field (200X) in 15 random sections from 3 different mice ( D ). Tissue extracts were analyzed for PCNA, cyclin D1, TGF-β1, and β-actin by Western blotting ( E ). Immunohistochemical staining of senescence-associated β-galactosidase (SA-β-gal) in hepatocytes. Scale bar, 100 μm ( F ). Fold-changes in IL-6 ( G ) and IL-8 ( H ) mRNA expression at 24 h after 70% PHx. Wild-type mice were intraperitoneally injected with vehicle (Veh) or chloroquine (CQ) at 0.5 h before PHx or the sham operation and then once per day until 48 h. Liver tissues were harvested at 0–48 h after surgery and tissue extracts were analyzed for PCNA, cyclin D1, TGF-β1, and β-actin by Western blotting ( I,J ). The values are shown as the mean ± SD in the bar graph and compared using Student’s t test. #P

    Article Snippet: Immunohistochemistry Liver paraffin sections from each sample were cut into 5-μm sections and stained with proliferation marker Ki67 rabbit antibody (Thermo Fisher Scientific, PA5-19462, Waltham, MA, USA) using the avidin-biotin-peroxidase complex technique (Vectastain ABC kit and DAB peroxidase substrate kit, Vector Laboratories, Burlingame, CA, USA).

    Techniques: Inhibition, Mouse Assay, Western Blot, Immunohistochemistry, Staining, Expressing, Injection

    Demonstration of expression of the goat uromodulin – GFP transgene and endogenous uromodulin in kidney sections by immunostaining. ( A ). H E kidney TAL region staining of a control non-transgenic mouse. The arrows show microstructures with thick walls indicative of the thick segment of the ascending limb of Henle's loop within the TAL region. Fixed kidney sections from a negative control mouse ( B ) and the 99-122-1-A5M transgenic mouse ( C ) were stained with a polyclonal anti-human uromodulin antibody, followed by treatment with Texas-Red conjugated secondary anti rabbit IgG antibody. Expression of endogenous uromodulin in the cells (red) appears in a punctuated pattern, whereas the GFP expression is cytoplasmic (green). Immunostaining of similar sections for uromodulin confirmed that expression is restricted to tubular epithelial cells of these structures ( B, C ) indicated by red. The same sections observed for GFP expression by confocal microscopy indicated that expression was restricted to similar structures as in B C but not co-expressed with the endogenous uromodulin staining. As expected GFP was absent in the renal sections obtained from a non-transgenic mouse. Both GFP ( D, F ) (green) and uromodulin ( E, F ) (red) were co-expressed in cells in the TAL segment (transgenic mouse, 99-122-1-A5M).

    Journal: BMC Biotechnology

    Article Title: Goat uromodulin promoter directs kidney-specific expression of GFP gene in transgenic mice

    doi: 10.1186/1472-6750-5-9

    Figure Lengend Snippet: Demonstration of expression of the goat uromodulin – GFP transgene and endogenous uromodulin in kidney sections by immunostaining. ( A ). H E kidney TAL region staining of a control non-transgenic mouse. The arrows show microstructures with thick walls indicative of the thick segment of the ascending limb of Henle's loop within the TAL region. Fixed kidney sections from a negative control mouse ( B ) and the 99-122-1-A5M transgenic mouse ( C ) were stained with a polyclonal anti-human uromodulin antibody, followed by treatment with Texas-Red conjugated secondary anti rabbit IgG antibody. Expression of endogenous uromodulin in the cells (red) appears in a punctuated pattern, whereas the GFP expression is cytoplasmic (green). Immunostaining of similar sections for uromodulin confirmed that expression is restricted to tubular epithelial cells of these structures ( B, C ) indicated by red. The same sections observed for GFP expression by confocal microscopy indicated that expression was restricted to similar structures as in B C but not co-expressed with the endogenous uromodulin staining. As expected GFP was absent in the renal sections obtained from a non-transgenic mouse. Both GFP ( D, F ) (green) and uromodulin ( E, F ) (red) were co-expressed in cells in the TAL segment (transgenic mouse, 99-122-1-A5M).

    Article Snippet: The samples were incubated with a mouse anti-rabbit biotin-conjugated antibody (0.75 % in modified PBS, Pierce) and rinsed.

    Techniques: Expressing, Immunostaining, Staining, Transgenic Assay, Negative Control, Confocal Microscopy

    PGN induced inhibition of Foxp3 expression is independent of IL-6, IL-6R, STAT1, STAT3, STAT4 and STAT6

    Journal: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

    Article Title: Distinct inflammatory signals have physiologically divergent effects on epigenetic regulation of Foxp3 expression and Treg function

    doi: 10.1111/j.1600-6143.2010.03389.x

    Figure Lengend Snippet: PGN induced inhibition of Foxp3 expression is independent of IL-6, IL-6R, STAT1, STAT3, STAT4 and STAT6

    Article Snippet: Purified CD4+ T cells were stained with APC conjugated anti-CD4 mAb, fixed with 3% paraformaldehyde, washed, permeabilized using 0.25% Triron-X-100 in PBS, and incubated with purified rabbit anti-IRF1 antibody (Santa Cruz Biotechnology) and biotin conjugated Foxp3 mAb (FKJ-16s; eBioscience).

    Techniques: Inhibition, Expressing

    IL-6 induces persistently decreased Foxp3 expression compared to TLR2

    Journal: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

    Article Title: Distinct inflammatory signals have physiologically divergent effects on epigenetic regulation of Foxp3 expression and Treg function

    doi: 10.1111/j.1600-6143.2010.03389.x

    Figure Lengend Snippet: IL-6 induces persistently decreased Foxp3 expression compared to TLR2

    Article Snippet: Purified CD4+ T cells were stained with APC conjugated anti-CD4 mAb, fixed with 3% paraformaldehyde, washed, permeabilized using 0.25% Triron-X-100 in PBS, and incubated with purified rabbit anti-IRF1 antibody (Santa Cruz Biotechnology) and biotin conjugated Foxp3 mAb (FKJ-16s; eBioscience).

    Techniques: Expressing

    TLR2 ligand inhibits Foxp3 expression

    Journal: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

    Article Title: Distinct inflammatory signals have physiologically divergent effects on epigenetic regulation of Foxp3 expression and Treg function

    doi: 10.1111/j.1600-6143.2010.03389.x

    Figure Lengend Snippet: TLR2 ligand inhibits Foxp3 expression

    Article Snippet: Purified CD4+ T cells were stained with APC conjugated anti-CD4 mAb, fixed with 3% paraformaldehyde, washed, permeabilized using 0.25% Triron-X-100 in PBS, and incubated with purified rabbit anti-IRF1 antibody (Santa Cruz Biotechnology) and biotin conjugated Foxp3 mAb (FKJ-16s; eBioscience).

    Techniques: Expressing

    PGN induced inhibition of Foxp3 expression and Treg mediated suppression is dependent on IRF-1

    Journal: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

    Article Title: Distinct inflammatory signals have physiologically divergent effects on epigenetic regulation of Foxp3 expression and Treg function

    doi: 10.1111/j.1600-6143.2010.03389.x

    Figure Lengend Snippet: PGN induced inhibition of Foxp3 expression and Treg mediated suppression is dependent on IRF-1

    Article Snippet: Purified CD4+ T cells were stained with APC conjugated anti-CD4 mAb, fixed with 3% paraformaldehyde, washed, permeabilized using 0.25% Triron-X-100 in PBS, and incubated with purified rabbit anti-IRF1 antibody (Santa Cruz Biotechnology) and biotin conjugated Foxp3 mAb (FKJ-16s; eBioscience).

    Techniques: Inhibition, Expressing

    IRF1 binds to the Foxp3 locus and negatively regulates Foxp3 expression

    Journal: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

    Article Title: Distinct inflammatory signals have physiologically divergent effects on epigenetic regulation of Foxp3 expression and Treg function

    doi: 10.1111/j.1600-6143.2010.03389.x

    Figure Lengend Snippet: IRF1 binds to the Foxp3 locus and negatively regulates Foxp3 expression

    Article Snippet: Purified CD4+ T cells were stained with APC conjugated anti-CD4 mAb, fixed with 3% paraformaldehyde, washed, permeabilized using 0.25% Triron-X-100 in PBS, and incubated with purified rabbit anti-IRF1 antibody (Santa Cruz Biotechnology) and biotin conjugated Foxp3 mAb (FKJ-16s; eBioscience).

    Techniques: Expressing

    Proximal tubule cells secrete pro-AOAH. (A) Diagram of AOAH structure, showing the conversion of the precursor (pro-AOAH) into mature AOAH. (B) Production of 35 S-AOAH by porcine proximal tubule cells in vitro. Labeled AOAH was immunoprecipitated as described in Methods and studied by SDS-PAGE and autoradiography. M, medium; L, lysate. Antibody +, anti-AOAH; antibody −, preimmune IgG. Only pro-AOAH is seen in the media, whereas the lysate contains both pro-AOAH and mature AOAH.

    Journal: Infection and Immunity

    Article Title: Identification of Acyloxyacyl Hydrolase, a Lipopolysaccharide- Detoxifying Enzyme, in the Murine Urinary Tract

    doi: 10.1128/IAI.72.6.3171-3178.2004

    Figure Lengend Snippet: Proximal tubule cells secrete pro-AOAH. (A) Diagram of AOAH structure, showing the conversion of the precursor (pro-AOAH) into mature AOAH. (B) Production of 35 S-AOAH by porcine proximal tubule cells in vitro. Labeled AOAH was immunoprecipitated as described in Methods and studied by SDS-PAGE and autoradiography. M, medium; L, lysate. Antibody +, anti-AOAH; antibody −, preimmune IgG. Only pro-AOAH is seen in the media, whereas the lysate contains both pro-AOAH and mature AOAH.

    Article Snippet: Wells were blocked for 1 h at 37°C, samples and recombinant human AOAH standard were incubated at 4°C overnight or at 37°C for 2 h, secondary antibody (E553, purified polyclonal rabbit anti-human AOAH, final concentration 10 μg/ml) was added for 1 h at 37°C, biotin-conjugated goat anti-rabbit IgG (0.075 μg/ml) (Zymed, San Francisco, Calif.) was added for 1 h at 37°C, 2 μg of alkaline phosphatase-conjugated streptavidin (Jackson Laboratories)/ml was incubated for 30 min at 37°C, and the plate was developed with alkaline phosphatase substrate (5 mM Sigma 104 and 0.1 M Sigma alkaline buffer 221 in water).

    Techniques: In Vitro, Labeling, Immunoprecipitation, SDS Page, Autoradiography