biotin conjugated goat anti rabbit  (Abcam)

 
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    Name:
    Goat Anti Rabbit IgG H L Biotin
    Description:

    Catalog Number:
    ab6720
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    None
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    Structured Review

    Abcam biotin conjugated goat anti rabbit

    https://www.bioz.com/result/biotin conjugated goat anti rabbit/product/Abcam
    Average 97 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    biotin conjugated goat anti rabbit - by Bioz Stars, 2020-09
    97/100 stars

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    Incubation:

    Article Title: miR-27a protects human mitral valve interstitial cell from TNF-α-induced inflammatory injury via up-regulation of NELL-1
    Article Snippet: .. Then, the PVDF membranes were incubated with goat anti-rabbit (1:5000 dilution, ab6720, Abcam) and goat anti-mouse (1:5000 dilution, ab6788, Abcam) secondary antibodies for 1 h at room temperature. ..

    Article Title: Folate Receptor-Positive Gynecological Cancer Cells: In Vitro and In Vivo Characterization
    Article Snippet: .. Slides were incubated with biotinylated secondary antibody (Abcam, ab97049, 1:200) for 30 min followed by addition of the Avidin-Biotin Complex kit (ABC Reagent kit, Elite, Vectastain, Vector Laboratories) and incubation for 30 min. DAB peroxidase substrate kit (Vector Laboratories, SK-4100) was used for the development of the signal and hematoxylin (Novolink™, Leica Biosystems) for counterstaining. .. Pictures were obtained using a light microscope (Axio Lab.A1, Zeiss, Oberkochen, Germany).

    Article Title: KLF5 promotes the tumorigenesis and metastatic potential of thyroid cancer cells through the NF-κB signaling pathway
    Article Snippet: .. The next day, sections were washed three times with PBS, incubated with goat anti-rabbit immunoglobulin G (IgG) second antibodies (dilution 1:1,000; cat. no. ab6720; Abcam, Cambridge, MA, USA) at 37°C for 30 min and visualized using the Metal Enhanced DAB Substrate kit (Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to the manufacturer's protocol. ..

    Avidin-Biotin Assay:

    Article Title: Folate Receptor-Positive Gynecological Cancer Cells: In Vitro and In Vivo Characterization
    Article Snippet: .. Slides were incubated with biotinylated secondary antibody (Abcam, ab97049, 1:200) for 30 min followed by addition of the Avidin-Biotin Complex kit (ABC Reagent kit, Elite, Vectastain, Vector Laboratories) and incubation for 30 min. DAB peroxidase substrate kit (Vector Laboratories, SK-4100) was used for the development of the signal and hematoxylin (Novolink™, Leica Biosystems) for counterstaining. .. Pictures were obtained using a light microscope (Axio Lab.A1, Zeiss, Oberkochen, Germany).

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  • 97
    Abcam biotin conjugated goat anti rabbit igg h l
    SDS-PAGE analysis of co-immunoprecipitation experiments under non-reducing condition. Panels:  a , receptor-mediated co-immunoprecipitation using hFasRECD-Fc;  b , antibody-mediated co-immunoprecipitation using biotin conjugated goat anti-rabbit IgG H  L. Lanes (in both panels): M, molecular-weight size markers; 1 and 2, Avidin-MTZ alone; 3 and 4, Avidin-hFasLECD conjugate; 5 and 6, hFasLECD-TCO alone; 7 and 8, rFab’-hFasLECD conjugate (the peak 1 fraction); 9 and 10, rFab’-hFasLECD conjugate (the combined fractions of peaks 2 and 3); 11 and 12, rFab’-MTZ alone; 1, 3, 5, 7, 9 and 11, examined samples; 2, 4, 6, 8, 10 and 12, precipitated materials (IP)
    Biotin Conjugated Goat Anti Rabbit Igg H L, supplied by Abcam, used in various techniques. Bioz Stars score: 97/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin conjugated goat anti rabbit igg h l/product/Abcam
    Average 97 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    biotin conjugated goat anti rabbit igg h l - by Bioz Stars, 2020-09
    97/100 stars
      Buy from Supplier

    97
    Abcam biotin conjugated goat anti rabbit
    SDS-PAGE analysis of co-immunoprecipitation experiments under non-reducing condition. Panels:  a , receptor-mediated co-immunoprecipitation using hFasRECD-Fc;  b , antibody-mediated co-immunoprecipitation using biotin conjugated goat anti-rabbit IgG H  L. Lanes (in both panels): M, molecular-weight size markers; 1 and 2, Avidin-MTZ alone; 3 and 4, Avidin-hFasLECD conjugate; 5 and 6, hFasLECD-TCO alone; 7 and 8, rFab’-hFasLECD conjugate (the peak 1 fraction); 9 and 10, rFab’-hFasLECD conjugate (the combined fractions of peaks 2 and 3); 11 and 12, rFab’-MTZ alone; 1, 3, 5, 7, 9 and 11, examined samples; 2, 4, 6, 8, 10 and 12, precipitated materials (IP)
    Biotin Conjugated Goat Anti Rabbit, supplied by Abcam, used in various techniques. Bioz Stars score: 97/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin conjugated goat anti rabbit/product/Abcam
    Average 97 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    biotin conjugated goat anti rabbit - by Bioz Stars, 2020-09
    97/100 stars
      Buy from Supplier

    Image Search Results


    SDS-PAGE analysis of co-immunoprecipitation experiments under non-reducing condition. Panels:  a , receptor-mediated co-immunoprecipitation using hFasRECD-Fc;  b , antibody-mediated co-immunoprecipitation using biotin conjugated goat anti-rabbit IgG H  L. Lanes (in both panels): M, molecular-weight size markers; 1 and 2, Avidin-MTZ alone; 3 and 4, Avidin-hFasLECD conjugate; 5 and 6, hFasLECD-TCO alone; 7 and 8, rFab’-hFasLECD conjugate (the peak 1 fraction); 9 and 10, rFab’-hFasLECD conjugate (the combined fractions of peaks 2 and 3); 11 and 12, rFab’-MTZ alone; 1, 3, 5, 7, 9 and 11, examined samples; 2, 4, 6, 8, 10 and 12, precipitated materials (IP)

    Journal: BMC Biotechnology

    Article Title: Site-specific chemical conjugation of human Fas ligand extracellular domain using trans-cyclooctene – methyltetrazine reactions

    doi: 10.1186/s12896-017-0381-2

    Figure Lengend Snippet: SDS-PAGE analysis of co-immunoprecipitation experiments under non-reducing condition. Panels: a , receptor-mediated co-immunoprecipitation using hFasRECD-Fc; b , antibody-mediated co-immunoprecipitation using biotin conjugated goat anti-rabbit IgG H L. Lanes (in both panels): M, molecular-weight size markers; 1 and 2, Avidin-MTZ alone; 3 and 4, Avidin-hFasLECD conjugate; 5 and 6, hFasLECD-TCO alone; 7 and 8, rFab’-hFasLECD conjugate (the peak 1 fraction); 9 and 10, rFab’-hFasLECD conjugate (the combined fractions of peaks 2 and 3); 11 and 12, rFab’-MTZ alone; 1, 3, 5, 7, 9 and 11, examined samples; 2, 4, 6, 8, 10 and 12, precipitated materials (IP)

    Article Snippet: Biotin conjugated goat anti-rabbit IgG H & L (ab207995) and ATTO495-Biotin were from Abcam Co. and ATTO-TEC GmbH, respectively.

    Techniques: SDS Page, Immunoprecipitation, Molecular Weight, Avidin-Biotin Assay

    Pilus-1 expression is regulated at the transcriptional level. A) High and low pilus expressing sub-populations of strains TIGR4 (Clade I), 19F Taiwan 14 (Clade I), OREP4 (Clade I), 6B Finland 14 (Clade II) and 35B SME 15 (Clade III) were labeled with clade specific anti-RrgB antibodies (1∶400 dilution) and FITC anti-mouse IgG secondary antibodies (1∶100 dilution). Pilus-1 expression was then analyzed by flow cytometry (FACS-Calibur). B) Schematic representation of PI-1. C) Log 2 ratio values indicating the PI-1 genes differential expression in High vs. Low pilus expressing sub-populations in the five above mentioned strains, as measured by spotted DNA microarray analysis. The data are measures of relative gene expression during in vitro growth in liquid cultures. The values reported for each gene are the mean of all the spots and their replicates within the array and of two independent experiments (bars represent standard deviations). D) Absolute gene expression levels of PI-1 genes measured for TIGR4 high and low pilus expressing sub-populations by microarray hybridization. Absolute expression levels reported for each gene are the mean of all the spots and their replicates within the array and of two independent experiments (bars represent the obtained standard deviations).

    Journal: PLoS ONE

    Article Title: The Streptococcus pneumoniae Pilus-1 Displays a Biphasic Expression Pattern

    doi: 10.1371/journal.pone.0021269

    Figure Lengend Snippet: Pilus-1 expression is regulated at the transcriptional level. A) High and low pilus expressing sub-populations of strains TIGR4 (Clade I), 19F Taiwan 14 (Clade I), OREP4 (Clade I), 6B Finland 14 (Clade II) and 35B SME 15 (Clade III) were labeled with clade specific anti-RrgB antibodies (1∶400 dilution) and FITC anti-mouse IgG secondary antibodies (1∶100 dilution). Pilus-1 expression was then analyzed by flow cytometry (FACS-Calibur). B) Schematic representation of PI-1. C) Log 2 ratio values indicating the PI-1 genes differential expression in High vs. Low pilus expressing sub-populations in the five above mentioned strains, as measured by spotted DNA microarray analysis. The data are measures of relative gene expression during in vitro growth in liquid cultures. The values reported for each gene are the mean of all the spots and their replicates within the array and of two independent experiments (bars represent standard deviations). D) Absolute gene expression levels of PI-1 genes measured for TIGR4 high and low pilus expressing sub-populations by microarray hybridization. Absolute expression levels reported for each gene are the mean of all the spots and their replicates within the array and of two independent experiments (bars represent the obtained standard deviations).

    Article Snippet: Depletion of RrgB positive bacteria TIGR4 low pilus expressing bacteria were incubated with rabbit anti-RrgB antibodies (1∶400 dilution) and then with goat anti-rabbit IgG biotin conjugated (Abcam, 1∶1000 dilution) antibodies.

    Techniques: Expressing, Labeling, Flow Cytometry, Cytometry, FACS, Microarray, In Vitro, Hybridization

    Pilus components display a biphasic expression pattern. A) TIGR4 bacteria were labeled with anti-RrgA, RrgB clade I, RrgC, BgaA (beta-galactosidase), PhtA (pneumococcal histidine triad protein A) or PspC (pneumococcal surface protein C, also known as CbpA,) primary antibodies (1∶400 dilution), and with FITC anti-mouse IgG secondary antibodies (1∶100 dilution). Bacterial staining was analyzed by flow cytometry (FACS-Calibur). Sera of mice immunized with PBS were used as negative control. B,C) TIGR4 bacteria were processed for immunofluorescence, stained with mouse anti-RrgB antibodies (1∶2000 dilution) (red) and with S. pneumoniae anti-capsular antibodies (Omniserum 1∶2000 dilution) (green). Imaging was performed with a confocal microscope. Scale bar is 4 µm in panel B and 1 µm in panel C.

    Journal: PLoS ONE

    Article Title: The Streptococcus pneumoniae Pilus-1 Displays a Biphasic Expression Pattern

    doi: 10.1371/journal.pone.0021269

    Figure Lengend Snippet: Pilus components display a biphasic expression pattern. A) TIGR4 bacteria were labeled with anti-RrgA, RrgB clade I, RrgC, BgaA (beta-galactosidase), PhtA (pneumococcal histidine triad protein A) or PspC (pneumococcal surface protein C, also known as CbpA,) primary antibodies (1∶400 dilution), and with FITC anti-mouse IgG secondary antibodies (1∶100 dilution). Bacterial staining was analyzed by flow cytometry (FACS-Calibur). Sera of mice immunized with PBS were used as negative control. B,C) TIGR4 bacteria were processed for immunofluorescence, stained with mouse anti-RrgB antibodies (1∶2000 dilution) (red) and with S. pneumoniae anti-capsular antibodies (Omniserum 1∶2000 dilution) (green). Imaging was performed with a confocal microscope. Scale bar is 4 µm in panel B and 1 µm in panel C.

    Article Snippet: Depletion of RrgB positive bacteria TIGR4 low pilus expressing bacteria were incubated with rabbit anti-RrgB antibodies (1∶400 dilution) and then with goat anti-rabbit IgG biotin conjugated (Abcam, 1∶1000 dilution) antibodies.

    Techniques: Expressing, Labeling, Staining, Flow Cytometry, Cytometry, FACS, Mouse Assay, Negative Control, Immunofluorescence, Imaging, Microscopy

    Pilus expression ratio is not correlated with serotype, genotype or clade type. Bacteria containing either PI-1 clade I (A), clade II (B) or clade III (C), were labeled with clade specific anti-RrgB antibodies (1∶400 dilution) and FITC anti-mouse IgG secondary antibodies (1∶100 dilution). Pilus-1 expression was then analyzed by flow cytometry (FACS-Calibur).

    Journal: PLoS ONE

    Article Title: The Streptococcus pneumoniae Pilus-1 Displays a Biphasic Expression Pattern

    doi: 10.1371/journal.pone.0021269

    Figure Lengend Snippet: Pilus expression ratio is not correlated with serotype, genotype or clade type. Bacteria containing either PI-1 clade I (A), clade II (B) or clade III (C), were labeled with clade specific anti-RrgB antibodies (1∶400 dilution) and FITC anti-mouse IgG secondary antibodies (1∶100 dilution). Pilus-1 expression was then analyzed by flow cytometry (FACS-Calibur).

    Article Snippet: Depletion of RrgB positive bacteria TIGR4 low pilus expressing bacteria were incubated with rabbit anti-RrgB antibodies (1∶400 dilution) and then with goat anti-rabbit IgG biotin conjugated (Abcam, 1∶1000 dilution) antibodies.

    Techniques: Expressing, Labeling, Flow Cytometry, Cytometry, FACS