biotin conjugated goat anti rabbit igg  (Cell Signaling Technology Inc)

 
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    Name:
    Anti rabbit IgG H L Biotinylated Antibody
    Description:
    Affinity purified goat anti rabbit IgG H L antibody is conjugated to biotin This product has been optimized for use as a secondary antibody in western blotting applications
    Catalog Number:
    14708
    Price:
    None
    Category:
    Secondary Antibodies
    Applications:
    Western Blot
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    Structured Review

    Cell Signaling Technology Inc biotin conjugated goat anti rabbit igg
    Affinity purified goat anti rabbit IgG H L antibody is conjugated to biotin This product has been optimized for use as a secondary antibody in western blotting applications
    https://www.bioz.com/result/biotin conjugated goat anti rabbit igg/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin conjugated goat anti rabbit igg - by Bioz Stars, 2021-06
    99/100 stars

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    Related Articles

    Immunoprecipitation:

    Article Title: Novel Regulatory Roles of Wnt1 in Infection-Associated Colorectal Cancer
    Article Snippet: .. Anti-Wnt1 was not immunoprecipitated by anti-IgG ( A , right panel ). ..

    Article Title: Novel Regulatory Roles of Wnt1 in Infection-Associated Colorectal Cancer
    Article Snippet: .. To evaluate ubiquitination of Wnt1, we did immunoprecipitation using anti-ubiquitin, anti-Wnt1, or anti-IgG (negative control) antibodies, respectively. ..

    Article Title: MicroRNAs Overcome Cell Fate Barrier by Reducing EZH2-Controlled REST Stability during Neuronal Conversion of Human Adult Fibroblasts
    Article Snippet: Crude nuclei were isolated in SONIC buffer (50mM HEPES (pH7.6), 140mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% Na-deoxyxhilate and 0.1% SDS) and were sonicated with probe-based sonicator. .. The sheared chromatin samples were immunoprecipitated overnight at 4 °C under gentle rotation with anti-IgG (CST, #2729), or anti-REST (Bethyl, A300-539) antibody, and then add ChIP-grade protein G magnetic beads to each IP reaction and incubate for 2h at 4 °C with rotati on. ..

    other:

    Article Title: Neuregulin-1 attenuates experimental cerebral malaria (ECM) pathogenesis by regulating ErbB4/AKT/STAT3 signaling
    Article Snippet: Rabbit antibody against STAT3, phospho-STAT3, rabbit antibody against AKT, phospho-AKT, anti-NeuN, anti-Iba1, and anti-glial fibrillary acidic protein (GFAP) were purchased from Cell Signaling Technology Inc. (Danvers, MA, USA).

    Chromatin Immunoprecipitation:

    Article Title: Toxoplasma gondii excreted‐secreted antigens suppress Foxp3 promoter activity via a SP1‐dependent mechanism. Toxoplasma gondii excreted‐secreted antigens suppress Foxp3 promoter activity via a SP1‐dependent mechanism
    Article Snippet: The chromatin was harvested and fragmented using enzymatic digestion and sonication. .. Chromatin immunoprecipitation was performed with anti‐histone H3 (CST), anti‐SP1 (Santa Cruz Biotechnology) or anti‐IgG (CST). .. Anti‐histone H3 antibody and mouse IgG were utilized as positive and negative control, respectively.

    Article Title: Ferroptosis is governed by differential regulation of transcription in liver cancer
    Article Snippet: The antibodies used for co-IP were anti-HIC1 (Sigma, #SAB1412231, and #SAB2103167) and anti-HNF4A (R & D Systems, PP-H6939-00), and the conventional co-IP protocols are available elsewhere. .. The antibodies used for ChIP were anti-H3K9Ac (Abcam, #ab4441), anti-KAT2B (Abcam, #ab12188), anti-HNF4A (R & D Systems, PP-H6939-00), anti-IgG (CST, #3900) and anti-HIC1 (Sigma, #SAB1412231). ..

    Article Title: MicroRNAs Overcome Cell Fate Barrier by Reducing EZH2-Controlled REST Stability during Neuronal Conversion of Human Adult Fibroblasts
    Article Snippet: Crude nuclei were isolated in SONIC buffer (50mM HEPES (pH7.6), 140mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% Na-deoxyxhilate and 0.1% SDS) and were sonicated with probe-based sonicator. .. The sheared chromatin samples were immunoprecipitated overnight at 4 °C under gentle rotation with anti-IgG (CST, #2729), or anti-REST (Bethyl, A300-539) antibody, and then add ChIP-grade protein G magnetic beads to each IP reaction and incubate for 2h at 4 °C with rotati on. ..

    Incubation:

    Article Title: Progranulin Gene Therapy Improves Lysosomal Dysfunction and Microglial Pathology Associated with Frontotemporal Dementia and Neuronal Ceroid Lipofuscinosis
    Article Snippet: The following day, the membranes were incubated in an IR-Dye-conjugated anti-mouse antibody (1:20 000; LI-COR Biotechnology). .. With each round of testing, one membrane was incubated with a sheep anti-mouse progranulin antibody (#AF2557; R & D Systems) or rabbit anti-GFP antibody (#2956; Cell Signaling Technology) as a positive control. .. The progranulin antibody was detected by incubation with a biotinylated anti-sheep antibody (Vector Laboratories), followed by IR-Dye 800-conjugated streptavidin (LI-COR Biotechnology), and the GFP antibody was detected with an IR-Dye 680LT-conjugated anti-rabbit secondary antibody (LI-COR Biotechnology).

    Positive Control:

    Article Title: Progranulin Gene Therapy Improves Lysosomal Dysfunction and Microglial Pathology Associated with Frontotemporal Dementia and Neuronal Ceroid Lipofuscinosis
    Article Snippet: The following day, the membranes were incubated in an IR-Dye-conjugated anti-mouse antibody (1:20 000; LI-COR Biotechnology). .. With each round of testing, one membrane was incubated with a sheep anti-mouse progranulin antibody (#AF2557; R & D Systems) or rabbit anti-GFP antibody (#2956; Cell Signaling Technology) as a positive control. .. The progranulin antibody was detected by incubation with a biotinylated anti-sheep antibody (Vector Laboratories), followed by IR-Dye 800-conjugated streptavidin (LI-COR Biotechnology), and the GFP antibody was detected with an IR-Dye 680LT-conjugated anti-rabbit secondary antibody (LI-COR Biotechnology).

    Negative Control:

    Article Title: Novel Regulatory Roles of Wnt1 in Infection-Associated Colorectal Cancer
    Article Snippet: .. To evaluate ubiquitination of Wnt1, we did immunoprecipitation using anti-ubiquitin, anti-Wnt1, or anti-IgG (negative control) antibodies, respectively. ..

    Magnetic Beads:

    Article Title: MicroRNAs Overcome Cell Fate Barrier by Reducing EZH2-Controlled REST Stability during Neuronal Conversion of Human Adult Fibroblasts
    Article Snippet: Crude nuclei were isolated in SONIC buffer (50mM HEPES (pH7.6), 140mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% Na-deoxyxhilate and 0.1% SDS) and were sonicated with probe-based sonicator. .. The sheared chromatin samples were immunoprecipitated overnight at 4 °C under gentle rotation with anti-IgG (CST, #2729), or anti-REST (Bethyl, A300-539) antibody, and then add ChIP-grade protein G magnetic beads to each IP reaction and incubate for 2h at 4 °C with rotati on. ..

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