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Becton Dickinson biotin conjugated goat anti rabbit igg
Biotin Conjugated Goat Anti Rabbit Igg, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotin conjugated goat anti rabbit igg/product/Becton Dickinson
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
biotin conjugated goat anti rabbit igg - by Bioz Stars, 2020-09
91/100 stars

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Incubation:

Article Title: Hepatitis C Virus Core Protein Leads to Immune Suppression and Liver Damage in a Transgenic Murine Model
Article Snippet: .. After the tissues were washed with phosphate-buffered saline containing 0.2% Tween, biotin-conjugated goat anti-rabbit immunoglobulin G (BD PharMingen) was applied at a 1:1,200 dilution for 30 min, followed by incubation with ABC complex (VECTOR laboratories, Burlingame, Calif.). .. The reaction was developed with diaminobenzidine (DAB) staining with nickel enhancement peroxidase (Vectastain ABC kit; VECTOR Laboratories) according to the manufacturer's instructions.

Labeling:

Article Title: Global increase in replication fork speed during a p57KIP2-regulated erythroid cell fate switch
Article Snippet: .. Combed DNA was denatured in 2 M hydrochloric acid and labeled with rat anti-BrdU (Abcam ab6326) and Alexa Fluor 594 goat anti-rat immunoglobulin G (IgG; Life Technologies A11007) to identify IdU tracks, with mouse anti-BrdU (BD Biosciences 347580) and Alexa Fluor 488 goat anti-mouse IgG (Invitrogen A11029) to identify CldU tracks, and with anti–single-stranded DNA rabbit IgG (Immuno-Biological Laboratories Co. Ltd. 18731), biotin-conjugated goat anti-rabbit IgG (BD Biosciences 550338), BV421 streptavidin (BioLegend 405226), and BV421 anti-human IgG (BioLegend 409317) to identify single-stranded DNA fibers. .. Fluorescence microscopy was carried out on a Zeiss Axioskop 40 fluorescence microscope.

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    Becton Dickinson fitc conjugated goat anti rabbit igg
    Flow cytometric analysis of ANP-positive cells among gastric mucosal cells. The percentage of ANP-positive cells was determined by flow cytometry with <t>FITC-conjugated</t> goat anti-rabbit <t>IgG.</t> A: Relative fluorescence intensity of gastric mucosal cells in the control and diabetic groups. M1 indicates the proportions of ANP-positive cells that displayed positive FITC labeling. The inserted fluorescent image is a single ANP-positive cell that had a positive fluorescent signal in its cytoplasm (× 400); B: ANP-positive cells among the gastric mucosal cells (10 4 cells per sample) in control and STZ-induced diabetic mice ( n = 3, a P
    Fitc Conjugated Goat Anti Rabbit Igg, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc conjugated goat anti rabbit igg/product/Becton Dickinson
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    fitc conjugated goat anti rabbit igg - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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    Flow cytometric analysis of ANP-positive cells among gastric mucosal cells. The percentage of ANP-positive cells was determined by flow cytometry with FITC-conjugated goat anti-rabbit IgG. A: Relative fluorescence intensity of gastric mucosal cells in the control and diabetic groups. M1 indicates the proportions of ANP-positive cells that displayed positive FITC labeling. The inserted fluorescent image is a single ANP-positive cell that had a positive fluorescent signal in its cytoplasm (× 400); B: ANP-positive cells among the gastric mucosal cells (10 4 cells per sample) in control and STZ-induced diabetic mice ( n = 3, a P

    Journal: World Journal of Gastroenterology : WJG

    Article Title: Atrial natriuretic peptide signal pathway upregulated in stomach of streptozotocin-induced diabetic mice

    doi: 10.3748/wjg.v16.i1.48

    Figure Lengend Snippet: Flow cytometric analysis of ANP-positive cells among gastric mucosal cells. The percentage of ANP-positive cells was determined by flow cytometry with FITC-conjugated goat anti-rabbit IgG. A: Relative fluorescence intensity of gastric mucosal cells in the control and diabetic groups. M1 indicates the proportions of ANP-positive cells that displayed positive FITC labeling. The inserted fluorescent image is a single ANP-positive cell that had a positive fluorescent signal in its cytoplasm (× 400); B: ANP-positive cells among the gastric mucosal cells (10 4 cells per sample) in control and STZ-induced diabetic mice ( n = 3, a P

    Article Snippet: The cells were stained with FITC-conjugated goat anti-rabbit IgG and examined by flow cytometry (Becton Dickinson).

    Techniques: Flow Cytometry, Aqueous Normal-phase Chromatography, Cytometry, Fluorescence, Labeling, Mouse Assay

    AvidinOX-anchored bTrast and bPert induce pro-apoptotic and reduce anti-apoptotic molecules in SKBR3 cells ( A ) Cells, with and without AvidinOX (AvOX) conjugation, were treated 72 hours with 1 μg/mL bTrast (upper panel) or 0.5 μg/mL bPert (lower panel), or with medium (Control). Expression of apoptosis-related proteins was measured on whole cell lysates by means of the Human Apoptosis Array kit. Representative images of the array membranes are shown. Spot pixel densities were recorded and analyzed by using image analysis software. Average background signal was subtracted from each spot and the normalized mean pixel densities of selected target proteins were plotted. Data (mean of duplicates ± SD) of selected target proteins were expressed as fold change with respect to baseline values (cells with or without AvidinOX). ( B ) Cells, with or without AvOX, were cultivated in the presence of bTrast (1 μg/mL) or bPert (0.5 μg/mL) for 72 hours and then fixed and stained with rabbit anti-Bcl-XL (yellow), rabbit anti-phospho-Bcl-XL (blue), rabbit anti-Bcl-2 (red) or mouse anti-cIAP2 (violet) followed by FITC-conjugated goat anti-rabbit IgG or goat anti-mouse IgG. Draq5 dye staining of nucleus and cytoplasm (grey). Fluorescence imaging by HCS Operetta. Each image is representative of at least 5 fields of duplicate wells. Magnification 60×. One representative experiment out of three.

    Journal: Oncotarget

    Article Title: AvidinOX-anchored biotinylated trastuzumab and pertuzumab induce down-modulation of ErbB2 and tumor cell death at concentrations order of magnitude lower than not-anchored antibodies

    doi: 10.18632/oncotarget.15145

    Figure Lengend Snippet: AvidinOX-anchored bTrast and bPert induce pro-apoptotic and reduce anti-apoptotic molecules in SKBR3 cells ( A ) Cells, with and without AvidinOX (AvOX) conjugation, were treated 72 hours with 1 μg/mL bTrast (upper panel) or 0.5 μg/mL bPert (lower panel), or with medium (Control). Expression of apoptosis-related proteins was measured on whole cell lysates by means of the Human Apoptosis Array kit. Representative images of the array membranes are shown. Spot pixel densities were recorded and analyzed by using image analysis software. Average background signal was subtracted from each spot and the normalized mean pixel densities of selected target proteins were plotted. Data (mean of duplicates ± SD) of selected target proteins were expressed as fold change with respect to baseline values (cells with or without AvidinOX). ( B ) Cells, with or without AvOX, were cultivated in the presence of bTrast (1 μg/mL) or bPert (0.5 μg/mL) for 72 hours and then fixed and stained with rabbit anti-Bcl-XL (yellow), rabbit anti-phospho-Bcl-XL (blue), rabbit anti-Bcl-2 (red) or mouse anti-cIAP2 (violet) followed by FITC-conjugated goat anti-rabbit IgG or goat anti-mouse IgG. Draq5 dye staining of nucleus and cytoplasm (grey). Fluorescence imaging by HCS Operetta. Each image is representative of at least 5 fields of duplicate wells. Magnification 60×. One representative experiment out of three.

    Article Snippet: FITC-conjugated goat anti-rabbit IgG (Becton Dickinson) or goat anti-mouse IgG (Becton Dickinson) were then added, according to the primary antibody used.

    Techniques: Conjugation Assay, Expressing, Software, Staining, Fluorescence, Imaging

    AvidinOX-anchored bTrast and bPert induce apoptosis and senescence of SKBR3 cells ( A ) Cells, with or without AvidinOX (AvOX), were stained with BrdU after 72-hour exposure to bTrast (1 μg/mL) or bPert (0.5 μg/mL) and then analyzed by flow cytometry. The Sub-G1 fraction of total cell population in grey. Representative results of one out of three independent samples. ( B ) HCS fluorescence imaging of cells cultivated 48 hours with bTrast (1 μg/mL) or bPert (0.5 μg/mL) then washed, fixed and stained with rabbit anti-cleaved caspase 3, followed by phycoherythrin-conjugated goat anti-Rabbit IgG (green). Draq5 dye staining of nucleus and cytoplasm (grey). Each image is representative of at least 5 fields of duplicate wells. Magnification 60x. Data are from one representative experiment out of two. ( C ) Caspase 3/7 activity in cells treated 72 hours with Pert or bPert at indicated concentrations, measured by Caspase-Glo 3/7 Assay. Data are expressed as fold change of activity compared to control cells and are the average of four replicates (± SE). Staurosporine (0.1 μg/mL) was included as positive control. Mann-Whitney's test: *** p ≤ 0.001, ** p ≤ 0.01 and * p ≤ 0.05 vs AvOX. ( D ) Cells were cultivated 6 days with antibodies as in B, and additional 3 days without antibodies. Cells in left panel were stained with rabbit anti-β-Galactosidase antibody followed by FITC-conjugated goat anti-rabbit IgG (violet). Cells in right panel were treated 1 hour with bafilomycin A1 and additional 2 hours with C 12 FDG substrate (green). Fluorescence imaging data analysis and staining of nucleus and cytoplasm as described above. Each image is representative of at least 5 fields of duplicate wells. Magnification 60×.

    Journal: Oncotarget

    Article Title: AvidinOX-anchored biotinylated trastuzumab and pertuzumab induce down-modulation of ErbB2 and tumor cell death at concentrations order of magnitude lower than not-anchored antibodies

    doi: 10.18632/oncotarget.15145

    Figure Lengend Snippet: AvidinOX-anchored bTrast and bPert induce apoptosis and senescence of SKBR3 cells ( A ) Cells, with or without AvidinOX (AvOX), were stained with BrdU after 72-hour exposure to bTrast (1 μg/mL) or bPert (0.5 μg/mL) and then analyzed by flow cytometry. The Sub-G1 fraction of total cell population in grey. Representative results of one out of three independent samples. ( B ) HCS fluorescence imaging of cells cultivated 48 hours with bTrast (1 μg/mL) or bPert (0.5 μg/mL) then washed, fixed and stained with rabbit anti-cleaved caspase 3, followed by phycoherythrin-conjugated goat anti-Rabbit IgG (green). Draq5 dye staining of nucleus and cytoplasm (grey). Each image is representative of at least 5 fields of duplicate wells. Magnification 60x. Data are from one representative experiment out of two. ( C ) Caspase 3/7 activity in cells treated 72 hours with Pert or bPert at indicated concentrations, measured by Caspase-Glo 3/7 Assay. Data are expressed as fold change of activity compared to control cells and are the average of four replicates (± SE). Staurosporine (0.1 μg/mL) was included as positive control. Mann-Whitney's test: *** p ≤ 0.001, ** p ≤ 0.01 and * p ≤ 0.05 vs AvOX. ( D ) Cells were cultivated 6 days with antibodies as in B, and additional 3 days without antibodies. Cells in left panel were stained with rabbit anti-β-Galactosidase antibody followed by FITC-conjugated goat anti-rabbit IgG (violet). Cells in right panel were treated 1 hour with bafilomycin A1 and additional 2 hours with C 12 FDG substrate (green). Fluorescence imaging data analysis and staining of nucleus and cytoplasm as described above. Each image is representative of at least 5 fields of duplicate wells. Magnification 60×.

    Article Snippet: FITC-conjugated goat anti-rabbit IgG (Becton Dickinson) or goat anti-mouse IgG (Becton Dickinson) were then added, according to the primary antibody used.

    Techniques: Staining, Flow Cytometry, Cytometry, Fluorescence, Imaging, Activity Assay, Caspase-Glo Assay, Positive Control, MANN-WHITNEY