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Agilent technologies biotin conjugated goat anti rabbit igg
Biotin Conjugated Goat Anti Rabbit Igg, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotin conjugated goat anti rabbit igg/product/Agilent technologies
Average 92 stars, based on 3 article reviews
Price from $9.99 to $1999.99
biotin conjugated goat anti rabbit igg - by Bioz Stars, 2020-09
92/100 stars

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Immunohistochemistry:

Article Title: Development of dextran sulphate sodium-induced experimental colitis is suppressed in genetically mast cell-deficient Ws/Ws rats
Article Snippet: .. For the immunohistochemical detection of MMC, the sections were incubated with a polyclonal rabbit anti-rat mast cell protease II (RMCP II; Moredun, Edinburgh, UK) antibody, followed by reaction with a biotin-conjugated goat anti-rabbit IgG and a peroxidase–streptavidin complex system (Dako, Glostrup, Denmark), as described previously [ , ]. .. RMCP II is regarded as a specific marker of rat MMC [ ].

Amplification:

Article Title: The Inner Foreskin of Healthy Males at Risk of HIV Infection Harbors Epithelial CD4+ CCR5+ Cells and Has Features of an Inflamed Epidermal Barrier
Article Snippet: .. Anti-CD4 (SP35, Cell Marque) and rabbit IgG were followed by biotin-conjugated goat anti-rabbit IgG (Dako) and amplified using Dako CSA/ABC Amplification Reagent, and streptavidin-AlexaFluor350. .. Anti-CCR5 (provided by M. Mack, University Hospital Regensburg, Germany) and mouse IgG (Jackson) were developed with goat anti-mouse AlexaFluor647.

Activity Assay:

Article Title: Docosahexaenoic acid preserves visual function by maintaining correct disc morphology in retinal photoreceptor cells
Article Snippet: .. Then, they were incubated with biotin-conjugated goat anti-rabbit IgG (Dako Japan, Tokyo, Japan) for 30 min at room temperature followed by the addition of alkaline phosphatase-conjugated streptavidin (Nichirei, Tokyo, Japan) for 5 min. Peroxidase activity was visualized by diaminobenzidine. .. For rhodopsin detection, sections were incubated with anti-rhodopsin mouse (GTX23267; GenTex, Rancho Cucamonga, CA) or negative control mouse IgG1 (Dako X0931) at 4 °C overnight.

Incubation:

Article Title: Oxaliplatin Neurotoxicity Involves Peroxisome Alterations. PPARγ Agonism as Preventive Pharmacological Approach
Article Snippet: .. Immuno-reaction was revealed by biotin-conjugated goat anti-rabbit IgG to a final dilution of 1∶200 (Dako, Glostrup, Denmark) followed by incubation with Streptavidin Peroxidase Complex (Thermo Scientific, Rancom Cheshire, UK). .. The morphology, size and cellular localization allowed us to identify the stained bodies as peroxisomes.

Article Title: Docosahexaenoic acid preserves visual function by maintaining correct disc morphology in retinal photoreceptor cells
Article Snippet: .. Then, they were incubated with biotin-conjugated goat anti-rabbit IgG (Dako Japan, Tokyo, Japan) for 30 min at room temperature followed by the addition of alkaline phosphatase-conjugated streptavidin (Nichirei, Tokyo, Japan) for 5 min. Peroxidase activity was visualized by diaminobenzidine. .. For rhodopsin detection, sections were incubated with anti-rhodopsin mouse (GTX23267; GenTex, Rancho Cucamonga, CA) or negative control mouse IgG1 (Dako X0931) at 4 °C overnight.

Article Title: Septoclast Deficiency Accompanies Postnatal Growth Plate Chondrodysplasia in the Toothless (tl) Osteopetrotic, Colony-Stimulating Factor-1 (CSF-1)-Deficient Rat and Is Partially Responsive to CSF-1 Injections
Article Snippet: .. After three washes in PBS, secondary antibody, biotin-conjugated goat anti-rabbit IgG (Dako, Carpenteria, CA), was applied and incubated for 1 hour at room temperature. .. After three washes in PBS, avidin-biotin-horseradish peroxidase complex (Dako) was applied according to the manufacturer’s instructions, the slides were washed with PBS, and diaminobenzidine substrate (Dako) was incubated on the sections to develop signal.

Article Title: FT011, a Novel Cardiorenal Protective Drug, Reduces Inflammation, Gliosis and Vascular Injury in Rats with Diabetic Retinopathy
Article Snippet: .. Sections were washed with PBS, incubated for 30 min with biotin-conjugated goat anti rabbit IgG (1:200, cat# E0432, DakoCytomation, Glostrup, Denmark), washed again and then incubated with the Vectastain ABC standard kit (Vector Laboratories) for 30 to 45 min and liquid DAB+substrate chromagen system (Dakocytomation) for 15 sec. ..

Article Title: Relief from neuropathic pain by blocking of the platelet-activating factor–pain loop
Article Snippet: .. The sections were incubated with biotin-conjugated goat anti-rabbit IgG (Dako, Tokyo, Japan) for 30 min at room temperature, followed by the addition of alkaline phosphatase–conjugated streptavidin (Nichirei, Tokyo, Japan) for 10 min. Color detection was performed with nitroblue-tetrazolium/5-bromo-4-chloro-3-indolyl phosphate solution (NBT/BCIP; Sigma-Aldrich) and the microscopic examinations were performed at GenoStaff. .. For the second IHC, tissue sections were incubated with anti-ionized calcium-binding adapter molecule 1 (Iba1) antibody (Wako) at 4°C overnight.

Article Title: Development of dextran sulphate sodium-induced experimental colitis is suppressed in genetically mast cell-deficient Ws/Ws rats
Article Snippet: .. For the immunohistochemical detection of MMC, the sections were incubated with a polyclonal rabbit anti-rat mast cell protease II (RMCP II; Moredun, Edinburgh, UK) antibody, followed by reaction with a biotin-conjugated goat anti-rabbit IgG and a peroxidase–streptavidin complex system (Dako, Glostrup, Denmark), as described previously [ , ]. .. RMCP II is regarded as a specific marker of rat MMC [ ].

Size-exclusion Chromatography:

Article Title: FT011, a Novel Cardiorenal Protective Drug, Reduces Inflammation, Gliosis and Vascular Injury in Rats with Diabetic Retinopathy
Article Snippet: .. Sections were washed with PBS, incubated for 30 min with biotin-conjugated goat anti rabbit IgG (1:200, cat# E0432, DakoCytomation, Glostrup, Denmark), washed again and then incubated with the Vectastain ABC standard kit (Vector Laboratories) for 30 to 45 min and liquid DAB+substrate chromagen system (Dakocytomation) for 15 sec. ..

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    Agilent technologies hrp conjugated rabbit anti goat igg
    Ionic strength–dependent interaction of CIP, with C3d assessed by ELISA. Microtiter wells were coated with 250 ng of C3d. The wells were probed with CIP or CD21 diluted in a buffer containing increasing concentrations of NaCl (0–2.5 M). Complex formation was revealed through anti-CIP or anti-CD21 <t>IgG</t> pAb, followed by an <t>HRP-conjugated</t> secondary antibody.
    Hrp Conjugated Rabbit Anti Goat Igg, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated rabbit anti goat igg/product/Agilent technologies
    Average 91 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated rabbit anti goat igg - by Bioz Stars, 2020-09
    91/100 stars
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    Ionic strength–dependent interaction of CIP, with C3d assessed by ELISA. Microtiter wells were coated with 250 ng of C3d. The wells were probed with CIP or CD21 diluted in a buffer containing increasing concentrations of NaCl (0–2.5 M). Complex formation was revealed through anti-CIP or anti-CD21 IgG pAb, followed by an HRP-conjugated secondary antibody.

    Journal: The FASEB Journal

    Article Title: The Streptococcus agalactiae complement interfering protein combines multiple complement-inhibitory mechanisms by interacting with both C4 and C3 ligands

    doi: 10.1096/fj.201801991R

    Figure Lengend Snippet: Ionic strength–dependent interaction of CIP, with C3d assessed by ELISA. Microtiter wells were coated with 250 ng of C3d. The wells were probed with CIP or CD21 diluted in a buffer containing increasing concentrations of NaCl (0–2.5 M). Complex formation was revealed through anti-CIP or anti-CD21 IgG pAb, followed by an HRP-conjugated secondary antibody.

    Article Snippet: The wells were washed 3 times with PBST, blocked with 2% (w/v) BSA in PBST for 1 h at 22°C, and then probed with C3d (100 ng/well) preincubated with serial dilutions of CIP in PBS, followed by incubation with anti-C3 goat pAb (1:2000; Complement Technology) and then HRP-conjugated rabbit anti-goat IgG (1:1000; Agilent Technologies).

    Techniques: Enzyme-linked Immunosorbent Assay

    Competitive inhibition assessment of CIP interaction with C3b and C4b. A ) Results of an experiment with 100 ng of C3b was coated on a 96-well plate and overlaid with 0.6 µM of CIP preincubated with equimolar amounts of C3b or C4b, followed by incubation with anti-CIP IgG pAb and then an anti-mouse HRP-conjugated antibody. B ) A similar experiment in which 100 ng of C4b was immobilized and overlaid with 0.6 µM of CIP preincubated with equimolar amounts of C4b or C3b.

    Journal: The FASEB Journal

    Article Title: The Streptococcus agalactiae complement interfering protein combines multiple complement-inhibitory mechanisms by interacting with both C4 and C3 ligands

    doi: 10.1096/fj.201801991R

    Figure Lengend Snippet: Competitive inhibition assessment of CIP interaction with C3b and C4b. A ) Results of an experiment with 100 ng of C3b was coated on a 96-well plate and overlaid with 0.6 µM of CIP preincubated with equimolar amounts of C3b or C4b, followed by incubation with anti-CIP IgG pAb and then an anti-mouse HRP-conjugated antibody. B ) A similar experiment in which 100 ng of C4b was immobilized and overlaid with 0.6 µM of CIP preincubated with equimolar amounts of C4b or C3b.

    Article Snippet: The wells were washed 3 times with PBST, blocked with 2% (w/v) BSA in PBST for 1 h at 22°C, and then probed with C3d (100 ng/well) preincubated with serial dilutions of CIP in PBS, followed by incubation with anti-C3 goat pAb (1:2000; Complement Technology) and then HRP-conjugated rabbit anti-goat IgG (1:1000; Agilent Technologies).

    Techniques: Inhibition, Incubation