biotin conjugated cona  (Millipore)

Journal: Frontiers in Chemistry

Article Title: Fluorescence Imaging of Extracellular Potassium Ion Using Potassium Sensing Oligonucleotide

doi: 10.3389/fchem.2022.922094

Figure Lengend Snippet: (A) Extracell imaging by 1 -StAv-ConA, (B) fluorescence ratio of F red/ F green (F red : F.I. of 550–640 nm,/F green : F.I. of 495–540 nm) after adding KCl, λ ex = 488 nm. (C) Extracell imaging by 1 -StAv-sulfo-NHS-biotin, (D) fluorescence ratio of F red/ F green after adding KCl, λ ex = 488 nm. (↑) indicates the change of DMEM medium.

Article Snippet: Two-hundred microliter of 0.5 μM ConA biotin conjugate (Type IV, SIGMA-ALDRICH, 4–8 mol biotin per mol protein) in DMEM (+10% FBS) was added in this dish and incubated for 10 min at 37°C under 5% CO 2 .

Techniques: Imaging, Fluorescence

Journal: Frontiers in Chemistry

Article Title: Fluorescence Imaging of Extracellular Potassium Ion Using Potassium Sensing Oligonucleotide

doi: 10.3389/fchem.2022.922094

Figure Lengend Snippet: (A) Cell surface imaging by 1 -StAv-ConA after adding 10 μM Amphotericin B, 10 μM Bumetanide, and 10 μM Ouabain/DMEM at 11 min (↑), (B) fluorescence ratio of F red/ F green (F red : F.I. of 575–595 nm,/F green : F.I. of 508–528 nm), λ ex = 488 nm in all cells. (C) Extracell imaging by 1 -StAv-sulfo-NHS-biotin and (D) fluorescence ratio of F red/ F green (F red : F.I. of 554–620 nm, F green : F.I. of 500–554 nm), λ ex = 488 nm after adding 26 μM Amphotericin B at 11 min (↑). The three cells shown in (C) were surrounded by ROI (region of interests), and their fluorescence ratios were shown in (D) .

Article Snippet: Two-hundred microliter of 0.5 μM ConA biotin conjugate (Type IV, SIGMA-ALDRICH, 4–8 mol biotin per mol protein) in DMEM (+10% FBS) was added in this dish and incubated for 10 min at 37°C under 5% CO 2 .

Techniques: Imaging, Fluorescence