anti mouse c3 ab  (Cedarlane)

Journal: Blood Advances

Article Title: Antibody-mediated immune suppression by antigen modulation is antigen-specific

doi: 10.1182/bloodadvances.2018018408

Figure Lengend Snippet: Antibody and complement deposition is detectable on HOD × KEL RBC posttransfusion, with clearance of HOD × KEL RBC occurring in anti-KEL immunized recipients. (A) HOD × KEL RBC were labeled before transfusion into PBS (no antibody), anti-KEL–, or anti-HEL–treated WT mice. (B) IgG deposition was measured by direct antiglobulin test at 1, 2, and 24 hours posttransfusion. Total C3 (C) and active complement (C3b/iC3b) (D) was measured at 1, 2, and 24 hours posttransfusion. (E) RBC survival of HOD × KEL+ compared with HOD × KEL− RBC at various times posttransfusion. Means ± standard deviation (SD) shown. (A-D) ****P < .0001, ***P < .001, **P < .01, and not significant (ns) by 1-way ANOVA with Tukey multiple comparison test. (E) ****P < .0001 and ns by 2-way ANOVA with Dunnett multiple comparisons test. Data shown include 5 mice per group and are representative of 3 independent experiments. DiI, 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate; DiO, 3,3′-dioctadecyloxacarbocyanine perchlorate; FSC, forward scatter; MFI, mean fluorescence intensity; SSC, side scatter.

Article Snippet: 24 , 38 - 40 Briefly, RBC were stained with APC anti-mouse IgG (Jackson Immunoresearch, West Grove, PA) for assessment of bound antibody, or with biotin anti-mouse complement component 3 (C3; Cedarlane, Burlington, NC) for assessment of total complement or biotin anti-mouse C3b (Cedarlane) for detection of active complement, followed by streptavidin APC (BD, Franklin Lakes, NJ).

Techniques: Labeling, Direct Antiglobulin Test, Standard Deviation, Fluorescence

Journal: Kidney International

Article Title: Complement receptor 3 mediates renal protection in experimental C3 glomerulopathy

doi: 10.1016/j.kint.2015.11.024

Figure Lengend Snippet: Plasma and glomerular C3 in mice with combined deficiency of factor h (FH) and complement receptor 3. ( a,b ) Plasma C3 levels ( a ) and glomerular C3 staining intensity ( b ) in 8-month-old Cfh –/– .Itgam –/– , Cfh –/– , and Itgam –/– mice housed in specific-pathogen free conditions. Horizontal bars denote median values. AFU, arbitrary fluorescent units. ( c ) Representative images of glomerular C3 immunostaining in the 3 genotypes. Bar = 80 μm. Each symbol represents a mouse. P values derived from Dunn’s multiple comparison test.

Article Snippet: For both iC3b-coated targets the level of iC3b opsonization was checked by flow cytometry using a biotinylated polyclonal antibody that recognizes both human and mouse C3 fragments (clone: 6C9, product no. CL7631B, Cedarlane Laboratories, Burlington, ON, Canada) followed by streptavidin-phycoerythrin (BD Biosciences Pharmingen).

Techniques: Staining, Immunostaining, Derivative Assay