biotin 14 datp  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Biotin 14 dATP
    Description:
    Biotin 14 dATP is a patented dATP analog with biotin attached at the 6 position of the purine base by a 14 atom linker The long 14 atom spacer arm allows accessibility to streptavidin for sensitive detection of probe target hybrids in in situ and filter based hybridizations Biotin 14 dATP can be efficiently incorporated into DNA by nick translation in the presence of dCTP dGTP and dTTP 1 Biotin 14 dATP can be added to the 3 end of DNA using terminal deoxynucleotidyl transferase 2 The amount supplied is sufficient for labeling up to 50 µg of DNA by nick translation Performance and Quality Testing Purity is evaluated by reverse phase HPLC analysis
    Catalog Number:
    19524016
    Price:
    None
    Applications:
    DNA & RNA Purification & Analysis|DNA Labeling|Nucleic Acid Labeling & Oligo Synthesis
    Category:
    Oligos Primers Probes Nucleotides
    Buy from Supplier


    Structured Review

    Thermo Fisher biotin 14 datp
    Schematic illustration of the principle of antarctic thermal-sensitive uracil-DNA-glycosylase-supplemented polymerase spiral reaction (ATSU-PSR) technique for eliminating carryover contamination. Two steps are needed for ATSU-PSR technique for removing carryover contamination. During the first stage, all PSR complicons labeled with dUTP in the presence of Bst 2.0 enzyme and dUTP. During the second stage, all subsequent ATSU-PSR amplifications are digested using ATSU, which specifically cleave carryover contaminants by removing uracil in amplicons from previous reactions. Importantly, ATSU is heat inactivated during the PSR amplification stage (63°C), and the digested contaminants are degraded, ensuring that only the target templates are amplified. In addition, three components, including fluorescein isothiocyanate (FITC)-labeled primer, <t>biotin-14-dCTP,</t> and <t>biotin-14-dATP,</t> are added into ATSU-PSR mixtures for forming the biotin- and FITC-attached duplex products.
    Biotin 14 dATP is a patented dATP analog with biotin attached at the 6 position of the purine base by a 14 atom linker The long 14 atom spacer arm allows accessibility to streptavidin for sensitive detection of probe target hybrids in in situ and filter based hybridizations Biotin 14 dATP can be efficiently incorporated into DNA by nick translation in the presence of dCTP dGTP and dTTP 1 Biotin 14 dATP can be added to the 3 end of DNA using terminal deoxynucleotidyl transferase 2 The amount supplied is sufficient for labeling up to 50 µg of DNA by nick translation Performance and Quality Testing Purity is evaluated by reverse phase HPLC analysis
    https://www.bioz.com/result/biotin 14 datp/product/Thermo Fisher
    Average 99 stars, based on 76 article reviews
    Price from $9.99 to $1999.99
    biotin 14 datp - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Simultaneous Nucleic Acids Detection and Elimination of Carryover Contamination With Nanoparticles-Based Biosensor- and Antarctic Thermal Sensitive Uracil-DNA-Glycosylase-Supplemented Polymerase Spiral Reaction"

    Article Title: Simultaneous Nucleic Acids Detection and Elimination of Carryover Contamination With Nanoparticles-Based Biosensor- and Antarctic Thermal Sensitive Uracil-DNA-Glycosylase-Supplemented Polymerase Spiral Reaction

    Journal: Frontiers in Bioengineering and Biotechnology

    doi: 10.3389/fbioe.2019.00401

    Schematic illustration of the principle of antarctic thermal-sensitive uracil-DNA-glycosylase-supplemented polymerase spiral reaction (ATSU-PSR) technique for eliminating carryover contamination. Two steps are needed for ATSU-PSR technique for removing carryover contamination. During the first stage, all PSR complicons labeled with dUTP in the presence of Bst 2.0 enzyme and dUTP. During the second stage, all subsequent ATSU-PSR amplifications are digested using ATSU, which specifically cleave carryover contaminants by removing uracil in amplicons from previous reactions. Importantly, ATSU is heat inactivated during the PSR amplification stage (63°C), and the digested contaminants are degraded, ensuring that only the target templates are amplified. In addition, three components, including fluorescein isothiocyanate (FITC)-labeled primer, biotin-14-dCTP, and biotin-14-dATP, are added into ATSU-PSR mixtures for forming the biotin- and FITC-attached duplex products.
    Figure Legend Snippet: Schematic illustration of the principle of antarctic thermal-sensitive uracil-DNA-glycosylase-supplemented polymerase spiral reaction (ATSU-PSR) technique for eliminating carryover contamination. Two steps are needed for ATSU-PSR technique for removing carryover contamination. During the first stage, all PSR complicons labeled with dUTP in the presence of Bst 2.0 enzyme and dUTP. During the second stage, all subsequent ATSU-PSR amplifications are digested using ATSU, which specifically cleave carryover contaminants by removing uracil in amplicons from previous reactions. Importantly, ATSU is heat inactivated during the PSR amplification stage (63°C), and the digested contaminants are degraded, ensuring that only the target templates are amplified. In addition, three components, including fluorescein isothiocyanate (FITC)-labeled primer, biotin-14-dCTP, and biotin-14-dATP, are added into ATSU-PSR mixtures for forming the biotin- and FITC-attached duplex products.

    Techniques Used: Labeling, Amplification

    Outline of nanoparticle-based biosensor-supplemented polymerase spiral reaction assay (NB-PSR). (A) Outline of PSR with Ft* primer, biotin-14-dCTP, and biotin-14-dATP. (B) The detailed structure of NB. (C) The schematic illustration of the principle of NB for visualization of PSR products. (D) Interpretation of the results: (I) positive (two red bands, including test line and control line, appeared on the visual region of NB); (II) negative (only the control line region showed a red band).
    Figure Legend Snippet: Outline of nanoparticle-based biosensor-supplemented polymerase spiral reaction assay (NB-PSR). (A) Outline of PSR with Ft* primer, biotin-14-dCTP, and biotin-14-dATP. (B) The detailed structure of NB. (C) The schematic illustration of the principle of NB for visualization of PSR products. (D) Interpretation of the results: (I) positive (two red bands, including test line and control line, appeared on the visual region of NB); (II) negative (only the control line region showed a red band).

    Techniques Used:

    2) Product Images from "Cytogenetic characterization and genome size of the medicinal plant Catharanthus roseus (L.) G. Don"

    Article Title: Cytogenetic characterization and genome size of the medicinal plant Catharanthus roseus (L.) G. Don

    Journal: AoB Plants

    doi: 10.1093/aobpla/pls002

    Cytogenetic analyses of C. roseus on mitotic spreads prepared from root tip cells . (A–C) Mitotic spreads of C. roseus stained with silver nitrate. (A) Prophase revealing a single nucleolus. (B) Prometaphase revealing a single pair of homologous chromosomes associated to the nucleolus. (C) Prometaphase with an already disorganized nucleolus and with the NORs restricted to a single chromosome pair. (D) Fluorescent staining with AD and DAPI of a prometaphase. (E) C-banding and staining with PI and DAPI of a prometaphase, highlighting the NORs in red. (F) Fluorescence in situ hybridization of rDNA of a metaphase. The probe (pTa71) was labelled with biotin-14-dATP and detected with fluorescein-avidin D, the chromosomes were counterstained with DAPI and the NORs are highlighted in green. Arrow indicates chromosome 8. (G) Catharanthus roseus karyotype. Chromosomes are aligned by the centromeric region and are ordered from 1 to 8, from the larger to the smaller, according to the morphometric analysis. (H) Ideogram of C. roseus chromosomes. Centromeres, constrictions, bands and NORs are depicted in the picture; the small arm of chromosome 8 is represented by a dashed line since it is usually not visible. Scale bars = 5 μm.
    Figure Legend Snippet: Cytogenetic analyses of C. roseus on mitotic spreads prepared from root tip cells . (A–C) Mitotic spreads of C. roseus stained with silver nitrate. (A) Prophase revealing a single nucleolus. (B) Prometaphase revealing a single pair of homologous chromosomes associated to the nucleolus. (C) Prometaphase with an already disorganized nucleolus and with the NORs restricted to a single chromosome pair. (D) Fluorescent staining with AD and DAPI of a prometaphase. (E) C-banding and staining with PI and DAPI of a prometaphase, highlighting the NORs in red. (F) Fluorescence in situ hybridization of rDNA of a metaphase. The probe (pTa71) was labelled with biotin-14-dATP and detected with fluorescein-avidin D, the chromosomes were counterstained with DAPI and the NORs are highlighted in green. Arrow indicates chromosome 8. (G) Catharanthus roseus karyotype. Chromosomes are aligned by the centromeric region and are ordered from 1 to 8, from the larger to the smaller, according to the morphometric analysis. (H) Ideogram of C. roseus chromosomes. Centromeres, constrictions, bands and NORs are depicted in the picture; the small arm of chromosome 8 is represented by a dashed line since it is usually not visible. Scale bars = 5 μm.

    Techniques Used: Staining, Fluorescence, In Situ Hybridization, Avidin-Biotin Assay

    Related Articles

    Magnetic Beads:

    Article Title: Disruption of Histone Modification and CARM1 Recruitment by Arsenic Represses Transcription at Glucocorticoid Receptor-Regulated Promoters
    Article Snippet: .. Magnetic Bead DNA A 1.8 kb Sph1/Nco1 fragment of the MMTV LTR from the pGEM3ZFM-LTRCAT plasmid that includes Nucs A-F of the MMTV promoter, was biotinylated (20 ug DNA fragment, 1x Klenow Buffer, 1 mM MgCl2 , 50 µM each dTTP aS, dGTP aS, dCTP aS (Axxora, San Diego, CA), 18 µM Biotin-14-dATP (Invitrogen Carlsbad, California), 10 U Klenow (Invitrogen, Carlsbad, California) at 25°C for 15 min and attached to streptavidin-coated magnetic beads using the Dynabead Kilobase Binder Kit (Dynal Biotech, Lake Success, NY) in 200 µl Kit binding buffer as described by Fletcher et al . ..

    Nick Translation:

    Article Title: Cytogenetic characterization and genome size of the medicinal plant Catharanthus roseus (L.) G. Don
    Article Snippet: .. Plasmid DNA was labelled by nick translation using biotin-14-dATP of the Bionick™ Labeling System (Invitrogen Life Technologies, Paisley, UK). .. Plasmid DNA (0.5–1 μg) was labelled using 50 μL of labelling reaction (using 5 μL of 10× enzyme mix) for 2 h at 16 °C, and the reaction was stopped with 5 μL of 500 mM EDTA (pH 8.0).

    Article Title: Chromosome Mapping of Repetitive Sequences in Rachycentron canadum (Perciformes: Rachycentridae): Implications for Karyotypic Evolution and Perspectives for Biotechnological Uses
    Article Snippet: .. The 18S rDNA probe was labeled by nick translation with DIG-11-dUTP, according to the manufacturer's specifications (Roche) and the 5S rDNA probe was labeled with biotin-14-dATP by nick translation, also according to the manufacturer's specifications (Bionick Labelling System, Invitrogen). .. The telomeric DNA sequence (TTAGGG)n was also used as a probe.

    Article Title: Karyotypic variation in the long-whiskered catfish Pimelodusblochii Valenciennes, 1840 ( Siluriformes, Pimelodidae) from the lower Tapajós, Amazonas and Trombetas Rivers
    Article Snippet: .. The PCR products were labeled by nick translation with biotin-14-dATP (BioNick Labeling System kit, Invitrogen/ThermoScientific, Waltham, Massachusetts, USA) and digoxigenin-11-dUTP (DIG-nick translation mix, Roche, Basel, Switzerland). ..

    Labeling:

    Article Title: Cytogenetic characterization and genome size of the medicinal plant Catharanthus roseus (L.) G. Don
    Article Snippet: .. Plasmid DNA was labelled by nick translation using biotin-14-dATP of the Bionick™ Labeling System (Invitrogen Life Technologies, Paisley, UK). .. Plasmid DNA (0.5–1 μg) was labelled using 50 μL of labelling reaction (using 5 μL of 10× enzyme mix) for 2 h at 16 °C, and the reaction was stopped with 5 μL of 500 mM EDTA (pH 8.0).

    Article Title: Chromosome Mapping of Repetitive Sequences in Rachycentron canadum (Perciformes: Rachycentridae): Implications for Karyotypic Evolution and Perspectives for Biotechnological Uses
    Article Snippet: .. The 18S rDNA probe was labeled by nick translation with DIG-11-dUTP, according to the manufacturer's specifications (Roche) and the 5S rDNA probe was labeled with biotin-14-dATP by nick translation, also according to the manufacturer's specifications (Bionick Labelling System, Invitrogen). .. The telomeric DNA sequence (TTAGGG)n was also used as a probe.

    Article Title: Karyotypic variation in the long-whiskered catfish Pimelodusblochii Valenciennes, 1840 ( Siluriformes, Pimelodidae) from the lower Tapajós, Amazonas and Trombetas Rivers
    Article Snippet: .. The PCR products were labeled by nick translation with biotin-14-dATP (BioNick Labeling System kit, Invitrogen/ThermoScientific, Waltham, Massachusetts, USA) and digoxigenin-11-dUTP (DIG-nick translation mix, Roche, Basel, Switzerland). ..

    Article Title: CDK inhibitor p21 is prosurvival in adriamycin-induced podocyte injury, in vitro and in vivo
    Article Snippet: .. Following incubation in One-Phor-All buffer (GE Healthcare, Piscataway, NJ), fragmented DNA was labeled by exposure of sections to diluted TdT (GE Healthcare) and biotin-14-dATP (Invitrogen, Grand Island, NY) for 60 min. ..

    Concentration Assay:

    Article Title: ISWI Remodeling Complexes in Xenopus Egg Extracts: Identification as Major Chromosomal Components that Are Regulated by INCENP-aurora B
    Article Snippet: .. To visualize the efficiency of DNA replication, biotin-14-dATP (GIBCO-BRL) was added at a final concentration of 4 μM. .. To convert the cell cycle state of the extract into mitosis, half a volume of mitotic LSS or recombinant cyclin B Δ90 was added ( ).

    Incubation:

    Article Title: CDK inhibitor p21 is prosurvival in adriamycin-induced podocyte injury, in vitro and in vivo
    Article Snippet: .. Following incubation in One-Phor-All buffer (GE Healthcare, Piscataway, NJ), fragmented DNA was labeled by exposure of sections to diluted TdT (GE Healthcare) and biotin-14-dATP (Invitrogen, Grand Island, NY) for 60 min. ..

    Polymerase Chain Reaction:

    Article Title: Karyotypic variation in the long-whiskered catfish Pimelodusblochii Valenciennes, 1840 ( Siluriformes, Pimelodidae) from the lower Tapajós, Amazonas and Trombetas Rivers
    Article Snippet: .. The PCR products were labeled by nick translation with biotin-14-dATP (BioNick Labeling System kit, Invitrogen/ThermoScientific, Waltham, Massachusetts, USA) and digoxigenin-11-dUTP (DIG-nick translation mix, Roche, Basel, Switzerland). ..

    Binding Assay:

    Article Title: Disruption of Histone Modification and CARM1 Recruitment by Arsenic Represses Transcription at Glucocorticoid Receptor-Regulated Promoters
    Article Snippet: .. Magnetic Bead DNA A 1.8 kb Sph1/Nco1 fragment of the MMTV LTR from the pGEM3ZFM-LTRCAT plasmid that includes Nucs A-F of the MMTV promoter, was biotinylated (20 ug DNA fragment, 1x Klenow Buffer, 1 mM MgCl2 , 50 µM each dTTP aS, dGTP aS, dCTP aS (Axxora, San Diego, CA), 18 µM Biotin-14-dATP (Invitrogen Carlsbad, California), 10 U Klenow (Invitrogen, Carlsbad, California) at 25°C for 15 min and attached to streptavidin-coated magnetic beads using the Dynabead Kilobase Binder Kit (Dynal Biotech, Lake Success, NY) in 200 µl Kit binding buffer as described by Fletcher et al . ..

    Plasmid Preparation:

    Article Title: Cytogenetic characterization and genome size of the medicinal plant Catharanthus roseus (L.) G. Don
    Article Snippet: .. Plasmid DNA was labelled by nick translation using biotin-14-dATP of the Bionick™ Labeling System (Invitrogen Life Technologies, Paisley, UK). .. Plasmid DNA (0.5–1 μg) was labelled using 50 μL of labelling reaction (using 5 μL of 10× enzyme mix) for 2 h at 16 °C, and the reaction was stopped with 5 μL of 500 mM EDTA (pH 8.0).

    Article Title: Disruption of Histone Modification and CARM1 Recruitment by Arsenic Represses Transcription at Glucocorticoid Receptor-Regulated Promoters
    Article Snippet: .. Magnetic Bead DNA A 1.8 kb Sph1/Nco1 fragment of the MMTV LTR from the pGEM3ZFM-LTRCAT plasmid that includes Nucs A-F of the MMTV promoter, was biotinylated (20 ug DNA fragment, 1x Klenow Buffer, 1 mM MgCl2 , 50 µM each dTTP aS, dGTP aS, dCTP aS (Axxora, San Diego, CA), 18 µM Biotin-14-dATP (Invitrogen Carlsbad, California), 10 U Klenow (Invitrogen, Carlsbad, California) at 25°C for 15 min and attached to streptavidin-coated magnetic beads using the Dynabead Kilobase Binder Kit (Dynal Biotech, Lake Success, NY) in 200 µl Kit binding buffer as described by Fletcher et al . ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher biotin 14 datp
    Schematic illustration of the principle of antarctic thermal-sensitive uracil-DNA-glycosylase-supplemented polymerase spiral reaction (ATSU-PSR) technique for eliminating carryover contamination. Two steps are needed for ATSU-PSR technique for removing carryover contamination. During the first stage, all PSR complicons labeled with dUTP in the presence of Bst 2.0 enzyme and dUTP. During the second stage, all subsequent ATSU-PSR amplifications are digested using ATSU, which specifically cleave carryover contaminants by removing uracil in amplicons from previous reactions. Importantly, ATSU is heat inactivated during the PSR amplification stage (63°C), and the digested contaminants are degraded, ensuring that only the target templates are amplified. In addition, three components, including fluorescein isothiocyanate (FITC)-labeled primer, <t>biotin-14-dCTP,</t> and <t>biotin-14-dATP,</t> are added into ATSU-PSR mixtures for forming the biotin- and FITC-attached duplex products.
    Biotin 14 Datp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin 14 datp/product/Thermo Fisher
    Average 99 stars, based on 83 article reviews
    Price from $9.99 to $1999.99
    biotin 14 datp - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Schematic illustration of the principle of antarctic thermal-sensitive uracil-DNA-glycosylase-supplemented polymerase spiral reaction (ATSU-PSR) technique for eliminating carryover contamination. Two steps are needed for ATSU-PSR technique for removing carryover contamination. During the first stage, all PSR complicons labeled with dUTP in the presence of Bst 2.0 enzyme and dUTP. During the second stage, all subsequent ATSU-PSR amplifications are digested using ATSU, which specifically cleave carryover contaminants by removing uracil in amplicons from previous reactions. Importantly, ATSU is heat inactivated during the PSR amplification stage (63°C), and the digested contaminants are degraded, ensuring that only the target templates are amplified. In addition, three components, including fluorescein isothiocyanate (FITC)-labeled primer, biotin-14-dCTP, and biotin-14-dATP, are added into ATSU-PSR mixtures for forming the biotin- and FITC-attached duplex products.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Simultaneous Nucleic Acids Detection and Elimination of Carryover Contamination With Nanoparticles-Based Biosensor- and Antarctic Thermal Sensitive Uracil-DNA-Glycosylase-Supplemented Polymerase Spiral Reaction

    doi: 10.3389/fbioe.2019.00401

    Figure Lengend Snippet: Schematic illustration of the principle of antarctic thermal-sensitive uracil-DNA-glycosylase-supplemented polymerase spiral reaction (ATSU-PSR) technique for eliminating carryover contamination. Two steps are needed for ATSU-PSR technique for removing carryover contamination. During the first stage, all PSR complicons labeled with dUTP in the presence of Bst 2.0 enzyme and dUTP. During the second stage, all subsequent ATSU-PSR amplifications are digested using ATSU, which specifically cleave carryover contaminants by removing uracil in amplicons from previous reactions. Importantly, ATSU is heat inactivated during the PSR amplification stage (63°C), and the digested contaminants are degraded, ensuring that only the target templates are amplified. In addition, three components, including fluorescein isothiocyanate (FITC)-labeled primer, biotin-14-dCTP, and biotin-14-dATP, are added into ATSU-PSR mixtures for forming the biotin- and FITC-attached duplex products.

    Article Snippet: Biotin-14-dCTP and biotin-14-dATP were obtained from Thermo Scientific Co., Ltd. (Shanghai, China).

    Techniques: Labeling, Amplification

    Outline of nanoparticle-based biosensor-supplemented polymerase spiral reaction assay (NB-PSR). (A) Outline of PSR with Ft* primer, biotin-14-dCTP, and biotin-14-dATP. (B) The detailed structure of NB. (C) The schematic illustration of the principle of NB for visualization of PSR products. (D) Interpretation of the results: (I) positive (two red bands, including test line and control line, appeared on the visual region of NB); (II) negative (only the control line region showed a red band).

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Simultaneous Nucleic Acids Detection and Elimination of Carryover Contamination With Nanoparticles-Based Biosensor- and Antarctic Thermal Sensitive Uracil-DNA-Glycosylase-Supplemented Polymerase Spiral Reaction

    doi: 10.3389/fbioe.2019.00401

    Figure Lengend Snippet: Outline of nanoparticle-based biosensor-supplemented polymerase spiral reaction assay (NB-PSR). (A) Outline of PSR with Ft* primer, biotin-14-dCTP, and biotin-14-dATP. (B) The detailed structure of NB. (C) The schematic illustration of the principle of NB for visualization of PSR products. (D) Interpretation of the results: (I) positive (two red bands, including test line and control line, appeared on the visual region of NB); (II) negative (only the control line region showed a red band).

    Article Snippet: Biotin-14-dCTP and biotin-14-dATP were obtained from Thermo Scientific Co., Ltd. (Shanghai, China).

    Techniques: